LOCUS       NZ_AP024209             2444 bp    DNA     circular CON 07-JUL-2023
DEFINITION  Escherichia coli strain TA8571 plasmid pTMTA85714, complete
            sequence.
ACCESSION   NZ_AP024209
VERSION     NZ_AP024209.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00260480
            Sequence Read Archive: DRR258151, DRR258152
            Assembly: GCF_016629485.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Ariyoshi,T., Kubota,H. and Sadamasu,K.
  TITLE     Complete genome sequences of Escherichia coli strain TA8571
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Ariyoshi,T., Aoki,K., Kubota,H., Yokoyama,K., Ishii,Y., Tateda,K.
            and Sadamasu,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2020) Contact:Tsukasa Ariyoshi Tokyo Metropolitan
            Institute of Public Health, Department of Microbiology; Hyakunincho
            3-24-1, Shinjuku-ku, Tokyo 169-0073, Japan URL
            :http://www.tokyo-eiken.go.jp/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 289x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/07/2023 04:45:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,739
            CDSs (total)                      :: 4,620
            Genes (coding)                    :: 4,394
            CDSs (with protein)               :: 4,394
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 65 of 226
            Pseudo Genes (incomplete)         :: 163 of 226
            Pseudo Genes (internal stop)      :: 35 of 226
            Pseudo Genes (multiple problems)  :: 38 of 226
            Pseudo Genes (short protein)      :: 5 of 226
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024209.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TA8571"
                     /db_xref="taxon:562"
                     /plasmid="pTMTA85714"
                     /geo_loc_name="Japan"
                     /collection_date="2018"
     gene            complement(join(2112..2444,1..618))
                     /gene="mobV"
                     /locus_tag="JG732_RS23325"
     CDS             complement(join(2112..2444,1..618))
                     /gene="mobV"
                     /locus_tag="JG732_RS23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_159181776.1"
                     /translation="MRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCSVSKSTD
                     QAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLEKKYGKD
                     RVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVKKLGLER
                     GIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAAIAKRLS
                     HAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQMTEILMM
                     AQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            1385..1825
                     /locus_tag="JG732_RS23330"
                     /old_locus_tag="ECTA8571_45490"
     CDS             1385..1825
                     /locus_tag="JG732_RS23330"
                     /old_locus_tag="ECTA8571_45490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000344119.1"
                     /translation="MDENGEVKQDSKIETFSIPAEPPYVKLYIADIAMIHGLSKSQNDV
                     LLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVDKQIFFRAGRGELEA
                     NPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVMDDEC"
                     /name="hypothetical protein"
     gene            1812..2042
                     /locus_tag="JG732_RS23335"
                     /old_locus_tag="ECTA8571_45500"
     CDS             1812..2042
                     /locus_tag="JG732_RS23335"
                     /old_locus_tag="ECTA8571_45500"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_124788667.1"
                     /translation="MMNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRT
                     SGNKYSTKGLSPKQKNAAFSILKKHNYHLND"
                     /name="hypothetical protein"
     Typing          complement(2116..2444)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.364"
                     /coverage="96.0"
                     /evalue="5.31e-42"
ORIGIN
        1 aatgcccttc cgcaatacac gcggctccag cgcctccggc gagatcgata cctggctacg
       61 ggtgccacgg tttatcgatt cgtagtagtg ctgaactgtc tgatgcgtgg cgcggcttcc
      121 ctcgatgccg cgctcaagac ccagtttttt tactgattca gcgtaggtgc tctgatcttc
      181 acgcatcttt gaccgcccgc caataaactc tttcgcggac agccgtccat cctgcgtcaa
      241 tggcaccacg aacgcagaca ggtgcggggt ggcctcgtcc cggtgaacca ccgccgccac
      301 aacacgatcc ttcccgtatt tcttctccag ccattgctca gagcgggcga aaaactccgc
      361 ctgctgccgt ggtgtggcct ctttccacca ctcagggctt gctgtcataa cgtattcaac
      421 ggccagtacc gcatctttac ggcgtttttc cggcagcagc tcccgcacac gccccattgc
      481 ctgatccgtt gatttggaca cagagcagta gttttctggt gtccgttcag cgttagcgtt
      541 cggcgtctca cgctctcggt agcagtgctg caacgcgctg gccacgcttc ccattcccgt
      601 cagctttttg cagcgcatta tcgcgtagtt cgccatgttc ccccctgatt ccggcattca
      661 ttcagccagg cgggggagcg cattgcgata cctccggcgg gccactatca agatgatagt
      721 gtaaactcac tagactttgc tacgcaaaat cgttcgggca gcaagctgcc agaaggtcaa
      781 aatcaacggc ggtctggcag gtggaaggcg tcgcttcgcg ccacccccca ccactgccaa
      841 gcctgtttta tccggagtgt cgtcaagggt aaatggcact cgctacgctc gcccttgacg
      901 aaatcaggca atgaaatcgt ctttgaaaga gcaggaaccg ctcgccgcag ctttggctga
      961 ggcgtaccgc aggagcaacg cgacgaggaa cgaacagcca aagcgaggaa gcggaatttc
     1021 cactccccgt acatagaaat gcgtacactc cttgctaaaa aaaggcagtt tttcagtgtc
     1081 taaaaatgcc gtttttcagc ccttattttc ccccttaata tatagatata gatatgggaa
     1141 cccgcttgta ccaagggcta gagcgaagaa tcgtactcac ctcaagtacc aatcgtactc
     1201 acctcaagta ctaatcgtac tcacctcaag gactaatcgt actcacctca agtactaatc
     1261 gtacttgata caaaggactt gactgtatgt ctctttagta cttaaatgcg agtactaaat
     1321 catggcgaaa cgtggggctg gagtgaatac gaaaaagatt tctaggcttg aacggcatac
     1381 atacatggat gaaaacggtg aggtaaaaca agactctaag atcgagacct tttccatccc
     1441 agccgaacct ccctacgtca agctatacat tgcagacatt gcgatgattc atggtttatc
     1501 aaaatctcaa aatgatgtgc ttttacaatt ggcaatgctg attggatggg atgggtttgt
     1561 gtctgtatca aaaactagat ttgaaaaaac catacagccc agggttcata ttgaatatca
     1621 gacctttaaa aacataattg cggctcttgt agataaacaa atattcttcc gggcagggcg
     1681 tggggaactt gaagctaacc cctttctttt tgcacgggga gactggccat caatttacga
     1741 aagaagaaag aaaattgagt taaagatcag ttatgacacc aatggaagaa cggtgcagac
     1801 gaaggtaatg gatgatgaat gctgaagata aactttttaa aaatgtagac aggctaatgc
     1861 tgcatgattc actaaaaaag aatgaggttc tgactgtttg ggagcgtact tttttaagca
     1921 gtgtttacag catatatcgt agaactagcg gtaataaata ctcaactaag ggtttgtcac
     1981 caaagcagaa aaacgcagct ttttctattt taaaaaaaca taattaccat ctgaatgatt
     2041 aattttttcg tggtgaatgc cgcaaatgac gtcactttgc ggcgttagcc ctatcctgca
     2101 tcgcatcaaa actaacgctc cataccaccc atgccacgcg atcgcgtctg tcgctgtttt
     2161 tcacgttcaa tacgctgctg tttttccttt tcctggtttt gctgcttaaa ccgctgagcc
     2221 atcatcagga tttcggtcat ctgctctctg gaaagcccct taaaaggctc tgtgacgctc
     2281 tgtaggcgtt tctgctgcgc atccatagtc ttctgtagct cacgcgctct ttttgcgttc
     2341 tgggcgctct gagaggccat agcgatggtt cctgcaaagc catcgttcac tgcgtggctt
     2401 agacgtttgg ctatggctgc ctgatcttcc acgtctttgg tgaa
//
