LOCUS       NZ_AP024180             3359 bp    DNA     circular CON 30-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KSB1_9A plasmid pKSB1_9A_7, complete
            sequence.
ACCESSION   NZ_AP024180
VERSION     NZ_AP024180.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07211281
            Sequence Read Archive: SRR5665581, SRR5665600
            Assembly: GCF_016629585.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Gomi,R.
  TITLE     Klebsiella pneumoniae isolate KSB1_9A genome sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Gomi,R. and Wyres,K.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2020) Contact:Ryota Gomi Kyoto University;
            KyotoDaigakuKatsura, Nishikyo-ku, Kyoto 615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore
            Technologies MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/30/2023 02:55:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,782
            CDSs (total)                      :: 5,660
            Genes (coding)                    :: 5,477
            CDSs (with protein)               :: 5,477
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 78 of 183
            Pseudo Genes (incomplete)         :: 120 of 183
            Pseudo Genes (internal stop)      :: 19 of 183
            Pseudo Genes (multiple problems)  :: 31 of 183
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024180.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB1_9A"
                     /db_xref="taxon:573"
                     /plasmid="pKSB1_9A_7"
                     /geo_loc_name="Australia"
     gene            complement(652..1053)
                     /locus_tag="JGR95_RS28720"
     CDS             complement(652..1053)
                     /locus_tag="JGR95_RS28720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_048270303.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKTGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNILLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1053..1244)
                     /locus_tag="JGR95_RS28725"
     CDS             complement(1053..1244)
                     /locus_tag="JGR95_RS28725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_049139654.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEELHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1678..>1758
                     /locus_tag="JGR95_RS29060"
                     /pseudo=""
     CDS             1678..>1758
                     /locus_tag="JGR95_RS29060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(2032..2790)
                     /locus_tag="JGR95_RS28730"
     CDS             complement(2032..2790)
                     /locus_tag="JGR95_RS28730"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181013984.1"
                     /translation="MALMVTRRYLLGGEYPSEEFVQASLEKYFFRQGFDIDTGSYIDLI
                     CRDKESRDVVWHIEVKGKTSQPGLDFRTCLGQLVQRMTKDNINYAIAVPRIKQYERLIE
                     ETSILAVSKLNLHWIFVGPDGSVTIKEPDSSTMKNGNGNGNGNGNGNGNGNGREVSLVN
                     GGFKRFITGDFMPTCSYGKAPVVFKSSRREMWDWLDDFVISNGVIPSIADIKKEAESRS
                     GKWTLSNVKIEYQFWKIYHLYKGHFFYQSE"
                     /name="hypothetical protein"
     gene            complement(2990..3160)
                     /locus_tag="JGR95_RS28735"
     CDS             complement(2990..3160)
                     /locus_tag="JGR95_RS28735"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157771094.1"
                     /translation="MATATNFDAWLDDVDGDYEEVMALYDSVQNVSDMGLYQCVEGGRG
                     DAWVVSSNHHP"
                     /name="hypothetical protein"
     Typing          1421..1805
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.487"
                     /coverage="89.0"
                     /evalue="2.97e-154"
ORIGIN
        1 cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg gcatgcatga gtatgcacgc
       61 aagtgcatag cgcgaaaatt cacgccattc agccagggta agtcctgttt ccggcgtact
      121 tcccatccga aaacggtgaa cggggacgca atttattgcg tggccgtgaa gcgttgccgg
      181 cgcagccgga aaaccggttt tcagcacgtt caggaatccg gttcgtcgac ggccggcaat
      241 cacgttatgg caagtcaccg aacgactcac cacacgattc accggcgact cttcactaga
      301 gtcgtaccca gagtcgccac gcctaaaaaa caaccaaccg gggagtcggg tctagagtcg
      361 ggtcgttatt aactcattgt ttttatagtc ctgagtcgtt accgactcta tgacgactcg
      421 ccggggactc tatgacgagt cgtcatagag tcggatctat ataaatcaaa tttaaaaaac
      481 gaaattttac agacttaaac tactgatatt aaatacaaat ccgcaaaatt ttaattttgc
      541 gtagtgtgtg gatattttta gtggtgagga gcgttcagaa atccggcgat tttttggaag
      601 gttcaggaac tccccgggat ggccggagag gaaaacggaa gcatgacggg gtcaggattt
      661 tttccagaat ttccaccatg gcgccgggga ttcagttaat tttgcctggt gattttcaag
      721 aaggagcatg gcctgtttca ggctgtcaat gtgcgaatcc ttggacaaaa gtaagatatt
      781 caggtgttca atctcctgtt tcagatgacg aatcacctgt tcactctggt cagcagaggc
      841 tgtatcacgc tgttcggaga cctgtccaga atggtgttca cccttcggtt gctcaaaggg
      901 accaaagaca cgtaaaagct ctgaagtatc taccattttt gcattgcgct caccactaca
      961 ggtggacagg tcacctgttt taattaacct gtgcagtgtt gttctactct tacccgtcag
     1021 tcgggcagcc tcactaatag acactctggc catcagttac catcctgtaa tgcatccagt
     1081 ctcgaggaca gtgtacggaa aagatgctcc gcatcatcgt ggagttcctc acaaaggtcg
     1141 gcctcgacat cgagatcaag ctcgttaagc ttctccagca ggagcagaga ctgattctgg
     1201 atgaatttag ccatgttcag cgctgttttc tgttgtttcg tcataagagg cctcagagcg
     1261 tcagtgctca atgtagggat gttctctttt ccgcttcctc gctcactcag tcgctacgct
     1321 cctgccgttc ggctgcggcg agcggcagga gcatgcgtat aaaagctcga gaaaaccgag
     1381 cgatcgcgtg atggggagaa ggagaggccg cacagcggcc gtttttccat aggctccgcc
     1441 ccccctgaca agcatcacag aaactgacgc tcaaatcagt ggtggcgaaa cccgacagga
     1501 ctataaagat atcaggcgtt tccccctgat ggctccctcg tgcgctctcc tgttccgacc
     1561 ctgccgctta ccagatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1621 agctcacgct gttggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1681 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1741 aacccggtaa gacacgacta atcgccattg acaacggcca ctggtagaca ggatgtgcga
     1801 gagatgtatc gagagttctt gaagtggtgg cctgactgcg gctacactga aaggacagat
     1861 ttggtgacat gtctcgcaca agactgttac cacggttcag aagttcccca acttactgaa
     1921 ccttcgaaaa accacctccc caggtggttt tttcgttttc cgagaaagag attacgcttt
     1981 gagcgaatga atctcaagaa gatcatggca tttgatgaac aaatgagtaa gctactcaga
     2041 ttgatagaaa aaatgcccct tatacaagtg atatattttc caaaactgat actctatttt
     2101 aacattagaa agcgtccatt tcccactccg cgattcagcc tcttttttaa tatcagcaat
     2161 acttgggatc accccatttg aaataacaaa atcgtccaac caatcccaca tttctcgccg
     2221 agatgattta aatacaacag gagctttccc atatgagcaa gttggcataa aatcccccgt
     2281 aataaatctt ttaaaccccc cattcacaag agaaacttct cttccattac cattaccatt
     2341 accattacca ttaccattac cattaccatt accatttttc atcgtagagc tatcaggttc
     2401 cttaattgtc acactgccat caggcccaac aaatatccag tgtaaattca atttactaac
     2461 agcaagaatg ctcgtttctt ctattaatct ttcatactgt tttattcttg gaacagcaat
     2521 agcataatta atattatctt ttgtcattct ctgcactaat tgtccaagac aagttcgaaa
     2581 atccaacccc ggctgtgatg tttttccttt tacttcaata tgccacacaa catccctaga
     2641 ttctttatca cgacaaataa ggtcaatata agaacccgta tctatatcga acccttgtcg
     2701 aaaaaaatat ttttctaaag aagcttgaac aaattcctcg gagggatatt caccacctag
     2761 taagtacctt ctcgtcacca taagagccac aacccctacg catcctattt actataacgt
     2821 catattttaa gagttcccac aaggggaact ctctgtacga ttacgagtga tcattcgata
     2881 tatttcgttg aaatccatac catgaatcaa catcttcgcc gccacatagc ctttctctaa
     2941 tcaatttgag gaaagtatcc ctagccacag ctgatgctag aaaaagagct tatggatgat
     3001 gattagagct cacgacccaa gcatcacccc gaccaccctc aacacactga tacaacccca
     3061 tatcacttac attctgaacg gaatcataaa gagccataac ctcctcataa tcgccatcaa
     3121 catcatctaa ccacgcatca aaattagtag cagtcgccat gccaatcctc cattttttag
     3181 ttttcaggag ttaatttaca tagtgatagg ggctgttaag caaataagtt tttacctttt
     3241 ttgcctcatg ctctaaaagt gagcaagaca ggcatttacc ataacgtccc attacgcgca
     3301 ccgccagaac ggcctcaggg cgtttctgac gggaaaccgg taaaacaggc aacaacccc
//
