LOCUS       NZ_AP024179             4941 bp    DNA     circular CON 30-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KSB1_9A plasmid pKSB1_9A_6, complete
            sequence.
ACCESSION   NZ_AP024179
VERSION     NZ_AP024179.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07211281
            Sequence Read Archive: SRR5665581, SRR5665600
            Assembly: GCF_016629585.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Gomi,R.
  TITLE     Klebsiella pneumoniae isolate KSB1_9A genome sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Gomi,R. and Wyres,K.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2020) Contact:Ryota Gomi Kyoto University;
            KyotoDaigakuKatsura, Nishikyo-ku, Kyoto 615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore
            Technologies MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/30/2023 02:55:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,782
            CDSs (total)                      :: 5,660
            Genes (coding)                    :: 5,477
            CDSs (with protein)               :: 5,477
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 78 of 183
            Pseudo Genes (incomplete)         :: 120 of 183
            Pseudo Genes (internal stop)      :: 19 of 183
            Pseudo Genes (multiple problems)  :: 31 of 183
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024179.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB1_9A"
                     /db_xref="taxon:573"
                     /plasmid="pKSB1_9A_6"
                     /geo_loc_name="Australia"
     gene            join(4664..4941,1..124)
                     /locus_tag="JGR95_RS28695"
     CDS             join(4664..4941,1..124)
                     /locus_tag="JGR95_RS28695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_024623230.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            853..1116
                     /locus_tag="JGR95_RS28700"
     CDS             853..1116
                     /locus_tag="JGR95_RS28700"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032434074.1"
                     /translation="MVNLNDICRSKKAAWIGGFFIFLAWQNGIFDTNSGWLLLRVQFLS
                     RTVLRAVLLYLQLLRYGMGMYEADRGFQTFFITPFFYQQTWI"
                     /name="hypothetical protein"
     gene            1964..2944
                     /locus_tag="JGR95_RS28705"
     CDS             1964..2944
                     /locus_tag="JGR95_RS28705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="WP_198562850.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNNEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGRVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRI
                     IKRQFGFVKARYRGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
                     /name="IS5-like element ISKpn26 family transposase"
     gene            3216..3635
                     /locus_tag="JGR95_RS28710"
     CDS             3216..3635
                     /locus_tag="JGR95_RS28710"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128336926.1"
                     /translation="MKLSLFFISVITFLLAISPIAYSDNTIIPDRVYDPYRNSAVTPDG
                     TVYDRLTGGVLKASEHPQPNFGFPVQSSSDSCDTTYVKARTEDGRLLVTSDGVTLHVVD
                     NIVDVAHWQPNDELRVCGDRVINIDDNTSSSFTYN"
                     /name="hypothetical protein"
     gene            4473..4664
                     /locus_tag="JGR95_RS28715"
     CDS             4473..4664
                     /locus_tag="JGR95_RS28715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032434073.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLTARLDALLDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(3730..4239)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.66"
                     /coverage="99.0"
                     /evalue="3.71e-149"
ORIGIN
        1 cttacttttg tccaaggatt cgcacattga cagcctgaaa caggccatgc tccttcttga
       61 aaatcaccag gcaaaattaa ctgaatcccc ggcgccatgg tggaaattct ggaaaaaatc
      121 ctgaccccgt catgcttcag ttttcctctc cggccatccc ggggagttcc tgaaccttcc
      181 caaaaatcgt cagatctctg aacgctcctc accactaaaa atacccacac cccacgcaaa
      241 attaacattt tgcagatttg tatttaatat cagtgggtta ctttcctgaa atttcgtttt
      301 ttaaatttta tttatataga tccgactcta tgacgagtcg tcatagagtc cccggcgagt
      361 cgtcatagag tcggtaacga ctcaggacta taaaaacaat aagttaataa cgacccgact
      421 ctagacacga ctccccgttt ggttgttttt tagacgcggc gactctaggt acgactctag
      481 tgaagagtcg ccggtgaatc gtgtggtgag tcgtccggcg acttgccata acgtgattgc
      541 cggccgtcga cgaacccgat tcctgaacgt gctgaaaacc ggttttccgg ctgcgccggc
      601 aacgcttcac ggccacgcaa taaattgcgt ccccgttcac cgttttcgga tgggaagtac
      661 gccggaaaca ggacttaccc tggctgaatg gcgtgaattt tcgcgctatg cacttgcgtg
      721 catactcatg catgccgtaa aaacagagcc tgcgcgtttc tggcgggttt tcgggtggtt
      781 tgttgcctgt tttaccggtt tcccctcaga aacgccctga gaccgtttta gcggtgcgta
      841 caagaagacg ttatggtaaa tctaaatgat atctgcagaa gcaaaaaagc cgcctggata
      901 ggcggctttt tcattttttt ggcgtggcag aacggcattt tcgacaccaa ctctggttgg
      961 ttgctcctga gggtacagtt cttaagccga acagtgctga gagctgtcct gctgtatttg
     1021 cagttgttac gttacggcat gggcatgtat gaggcagacc gaggtttcca gacattcttc
     1081 atcactccat ttttttacca acaaacgtgg atatgactgc acccaaggcg atcatgccta
     1141 atgtgccgac tccgccgcca atacctgatg tacatgacgg acacaactca tcgtcataca
     1201 aacgaccacc cttagcgtaa ttctcacacc ccggagcgcg acatatgtaa attgcttctt
     1261 tgcactcagg gcattcgcac ttatctcgtc gtacatggtt ttcttgaatt acacgaggta
     1321 taacttcagc tttacaaaaa ggacataacc ctttgtgttt tgatgtcatt ttgacctact
     1381 taattgcact gcaactaatt aattttgaac gacatttagg gcagtagttc ggtgttccaa
     1441 acccactcct actagtccat tcatgccgac aagccatgca tttagcccga tagataacaa
     1501 caaccgccat gaaaaaccac cattaatatc agtaactaat attcatttat aataactgac
     1561 aaactaaaca agtaaagtaa attgtgtatt tttttaagtg attgattttt aatttatttt
     1621 tctattatct aaaggctgta aattttaatt aatgcattaa atacaatgac ttaatctgat
     1681 ttgttttttt tgtttaactt tttggagggc gagaaagact catgcatctc gttcgtgcgc
     1741 tcatgcagcg taacgattgt tagcatacaa gaagtgcggg gctgggatgt tatgtttaat
     1801 ctgataagaa cggaatgttt tatggaaagg gtattaggaa tttatgttgt cggtattatt
     1861 gtgagtgccg gtttcctgtt tatccaagaa gttagggaag gtgtgaacaa gttcctgata
     1921 tgagatcatc atattcatcc ggagcgcatc ccagagggac atcatgagcc atcaactcac
     1981 cttcgccgat agtgaattca gcactaagcg ccgtcagacc cgaaaagaga ttttcctctc
     2041 ccgcatggag cagattctgc catggcagaa tatgaccgct gtcatcgagc cgttttatcc
     2101 caaggcgggc aatggccgac ggccctatcc gctggagacc atgctgcgta ttcactgcat
     2161 gcagcattgg tacaacctga gcgacggtgc catggaagat gccctgtacg aaatcgcctc
     2221 catgcgcctg tttgcccgat tatccctgga tagcgccctg ccggatcgca ccaccatcat
     2281 gaatttccgc cacctgctcg agcagcatca actggcccgt caattgttca agaccatcaa
     2341 tcgctggctg gccgaagcag gcgtcatgat gacccaaggc actttggtgg atgccaccat
     2401 cattgaggca cccagctcta ccaagaacaa tgagcagcaa cgcgatccgg agatgcatca
     2461 gaccaagaaa ggcaatcagt ggcactttgg catgaaggcc cacattggtg tcgatgccaa
     2521 gagtggcctg acccacagcc tggtcaccac cgcggccaac gagcatgacc tcaatcagct
     2581 gggtaatctg cttcatggag aggagcaatt tgtctcagcc gatgccggct accaaggagc
     2641 gccacagcgc gaggagctgg ccgaggtgga tgtggactgg ctgatcgccg agcgtcccgg
     2701 cagggtaaaa accttgaagc agcatccgcg caagaacaaa acggccatca acatcgaata
     2761 catgaaagcc agcatccgtg ccagggtgga gcacccgttt cgcatcatca agcggcagtt
     2821 cggcttcgtg aaagccagat acagggggct gctgaaaaac gataaccaac tggcgatgtt
     2881 attcaccctg gccaacctgt ttcgggtgga ccaaatgata cgtcagtggg agagatctca
     2941 gtaaaaaccg gaaataacgc cagaaatggt ggaaaaaata gcctaaatag gctgattcga
     3001 tgtgtttgcg ggaaaaaaat cggcccagat ccgcgaaatt ttaatcagcg agtcagcttg
     3061 ggaagaaatg acctgcttat tcgcaccttc cttaggaacg ggaggcgcag aagttacacc
     3121 gaagaaggtg cgtcaatgct tgtcggcata atttgtgcgt ttttgtggcc tttagtttta
     3181 attgcatatt tttttgataa aaaaaatggg aaattatgaa gttaagtctg tttttcatct
     3241 cggtcattac ttttttattg gctatatcac cgatagcata ctcagataac acgattattc
     3301 ctgacagagt atatgatcct tatagaaatt cggctgtaac tcctgatggc accgtttatg
     3361 accgtctgac aggaggagtt ttgaaggcat cagagcaccc tcaacctaat tttgggtttc
     3421 ctgttcaatc ctcatcggat agttgtgata caacgtatgt taaagctagg acagaagacg
     3481 gtaggctttt agtcacgagc gacggagtaa ctctgcatgt agttgacaat attgttgatg
     3541 ttgctcactg gcaaccaaat gatgagctta gagtctgtgg cgatagagta atcaatattg
     3601 atgacaatac ttcctcctcg ttcacataca attaacctct tttattcaaa gaatatctat
     3661 ggtgatgata gaaagattga cgagcatgta tggggggata gccgaagaaa tgaaccaaag
     3721 ttcaaaagga tcttcttgag atcctttcag gagcgtcgta atctcttgct ctgaaaacga
     3781 aaaaaccacc tggggaggtg gttttccgaa ggttcagtaa gttggggaac ttctgaaccg
     3841 tggtaacagg gtgtacaaga ccgctgccac caaattcgtc ctttcagttt agccgcagtc
     3901 tggctctaac ttcaagaact ctgcatcagt aatctcttgt acaccctcta ccaatggcta
     3961 ccgccaatgg cgatttgtcg tgccttttcg ggttggactc aagacgatag ttaccgaaaa
     4021 gagcgtggta gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
     4081 ccttcccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg
     4141 acaccggcaa accgaaaggc aggaacagga gagcgcacga gggagccatc agggggaaac
     4201 gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagattccg
     4261 tgatgctcgt caggggggcg gagcctatgg aaaaacggcc gcgcggcggc cttcccttct
     4321 ttcctccatg cgattactcg gttttctcga gcgtttctac gcatgctcct gccgctcgcc
     4381 gcagccgaac ggcaggagcg tagcgactga gtgagcgagg aagcggaaaa gaggacatcc
     4441 ctacactgag cactgacgct ctgaggcctc ttatgacgaa acaacagaaa acagcgttga
     4501 acatggctaa attcatccag aatcagtctc tgctcctgct ggagaagctt aacgagcttg
     4561 atctcgatgt cgaagccgac ctttgtgaga aactccacga tgatgcggag catcttttcc
     4621 gtacactgac cgcgagactg gatgcattac tggatggtaa ctgatggcca gagtgtctat
     4681 tagtgaggct gcccgactga cgggtaagag tagaacaaca ctgcacaggt taattaaagc
     4741 aggtgacctg tccacctgta gcggtgaacg taatgcaaaa atggtagata cttcagagct
     4801 tttacgtgtc tttggcccct ttgaacaact gaagggtgaa caccattctg gacaggtctc
     4861 tgaacagcgt gatacagcct ctgctgacca gagtgaacag gtgattcgtc atctgaaaca
     4921 ggagattgaa cacctgaaca c
//
