LOCUS       NZ_AP024141             5012 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Aeromonas caviae strain KAM329 plasmid pKAM329_5, complete sequence.
ACCESSION   NZ_AP024141
VERSION     NZ_AP024141.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00254948
            Assembly: GCF_015508545.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas caviae
  ORGANISM  Aeromonas caviae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Suzuki,M., Maehana,S. and Kitasato,H.
  TITLE     Whole genome sequencing of carbapenem-resistant Aeromonas spp.
            isolated in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Suzuki,M., Maehana,S. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2020) Contact:Masato Suzuki National Institute of
            Infectious Diseases; 4-2-1 Aobacho, Higashimurayama, Tokyo 189-0002,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.4.8.0; Canu v. 1.8
            Genome Coverage                   :: 192x
            Sequencing Technology             :: Illumina MiniSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015508545.1-RS_2024_03_23
            Annotation Date                   :: 03/23/2024 01:47:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,922
            CDSs (total)                      :: 4,764
            Genes (coding)                    :: 4,613
            CDSs (with protein)               :: 4,613
            Genes (RNA)                       :: 158
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 122
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 38 of 151
            Pseudo Genes (incomplete)         :: 115 of 151
            Pseudo Genes (internal stop)      :: 22 of 151
            Pseudo Genes (multiple problems)  :: 20 of 151
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024141.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Aeromonas caviae"
                     /mol_type="genomic DNA"
                     /strain="KAM329"
                     /db_xref="taxon:648"
                     /plasmid="pKAM329_5"
                     /geo_loc_name="Japan:Kanagawa"
                     /collection_date="2018"
     gene            complement(join(4988..5012,1..344))
                     /locus_tag="JN439_RS24720"
     CDS             complement(join(4988..5012,1..344))
                     /locus_tag="JN439_RS24720"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_234927011.1"
                     /translation="MIGDLFFDGGLVVVALVRLEIWPQQPRPERVIWPRPALFVVVFVA
                     HGVEVFALTWWGWIERLPCAQVNAGDHDVEMYPAIRFFMLNGCQVDVLPIQSGKGQRFE
                     VVQYRADFLRPRRFFNSP"
                     /name="hypothetical protein"
     gene            505..1041
                     /locus_tag="JN439_RS24725"
                     /old_locus_tag="KAM329_47990"
     CDS             505..1041
                     /locus_tag="JN439_RS24725"
                     /old_locus_tag="KAM329_47990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="WP_223938704.1"
                     /translation="MEHLGPQKTRLSERLQQQQEQALGEVETLAKAVLEALKNDLQQRV
                     NGELDTTVTAIRKGIEGLPKQITDSSQPLQAALEQAQSEANTLKLVSSVWLKSLLIGLC
                     TAVGIGLGSWGVLSWMGSEVQRLNSEISSAKATLAQLPKGVSYAKGEDGKGYILAPSMG
                     TPFETKSGKMAVEVN"
                     /name="DUF1640 domain-containing protein"
     gene            1042..1350
                     /locus_tag="JN439_RS24730"
                     /old_locus_tag="KAM329_48000"
     CDS             1042..1350
                     /locus_tag="JN439_RS24730"
                     /old_locus_tag="KAM329_48000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_223918476.1"
                     /translation="MTELERMLLDRLERIETAHRQQTAALEQQLQQQARSLNELQTACT
                     SALASCGTLCSELQHEFETLRNGVDRSNRATTTALGSLSRSVNDLSKALDALHRAQR"
                     /name="mobilization protein"
     gene            1531..2013
                     /locus_tag="JN439_RS24425"
                     /old_locus_tag="KAM329_48010"
     CDS             1531..2013
                     /locus_tag="JN439_RS24425"
                     /old_locus_tag="KAM329_48010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020411068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202212059.1"
                     /translation="MRMKLTGLLCCVALTGCATAYQPYSYFGGGGYSDVQLAENVFQVT
                     VEANGFTTSTEAADLALLRSADLTLERGFKYFIIGSTADHSYSAGYTTPSTTTLNATSY
                     GNTIYGTAQTTGGQTYVFNFPTPSMTITCFSDQPALNGTVYDAAITSRSLRAKLGI"
                     /name="hypothetical protein"
     gene            2105..2344
                     /locus_tag="JN439_RS24430"
                     /old_locus_tag="KAM329_48020"
     CDS             2105..2344
                     /locus_tag="JN439_RS24430"
                     /old_locus_tag="KAM329_48020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="WP_202212060.1"
                     /translation="MVAAAMRSKRTVSVTLEPSLLERARAAGINLSATLATALQHEING
                     SEAERWKRENRAAIQTLNRMVEERGLFSDDHRTF"
                     /name="type II toxin-antitoxin system CcdA family
                     antitoxin"
     gene            2344..2652
                     /locus_tag="JN439_RS24435"
                     /old_locus_tag="KAM329_48030"
     CDS             2344..2652
                     /locus_tag="JN439_RS24435"
                     /old_locus_tag="KAM329_48030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="WP_202212061.1"
                     /translation="MQFTIYRNKGNARDYPYLLNVQSDIIGELHTRMVIPLVSLADFPG
                     RPAQRLNPVITVEGGKYLVLTHEMAAVRLAQLGEEVTDVQESRQLIKGAIDFLLDGV"
                     /name="CcdB family protein"
     gene            2803..3750
                     /locus_tag="JN439_RS24440"
                     /old_locus_tag="KAM329_48040"
     CDS             2803..3750
                     /locus_tag="JN439_RS24440"
                     /old_locus_tag="KAM329_48040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_202212055.1"
                     /translation="MTNLAQLDLFRERLPRKPYYTDELTTGLHIADIARALGSRYIQPN
                     GPTHKHWLVFDVDHSAATLSWDDVGAPAPNITATNRDNGHAHLIYGLEIPVRTAPDGKV
                     APLRYAAAIEAALRELLGADLGYSGLICKNPLHSHWLVQVWEPQTYTLDWLADYLDLSP
                     YNGRKHLPAYGLGRNCTLFEKTRLWAYKAIRQGWPGFDTWQAAVIERASGYNVQFEQPL
                     PANEVRHTAKSIAKWTHRNLTPAGFSAVQAARGAKGGRIGGKVSKGGGRPSKAAELLPE
                     VLRLKEMGYSNRDIAEDLQISAGSVSNYLRRDHE"
                     /name="replication initiation protein"
     gene            complement(3902..4192)
                     /locus_tag="JN439_RS24445"
                     /old_locus_tag="KAM329_48050"
     CDS             complement(3902..4192)
                     /locus_tag="JN439_RS24445"
                     /old_locus_tag="KAM329_48050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202212056.1"
                     /translation="MKDTKDQGTGDMFGTKRGRGRPKTGAAKSGAERQAAYRAKQQDNN
                     VTVTINRALMEGLAAQMQAIRDGGSAVVLTQEQAGEILRALRTAETKQLRG"
                     /name="hypothetical protein"
     gene            4402..4812
                     /locus_tag="JN439_RS24450"
                     /old_locus_tag="KAM329_48060"
     CDS             4402..4812
                     /locus_tag="JN439_RS24450"
                     /old_locus_tag="KAM329_48060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_202212057.1"
                     /translation="MSKAPRTTTVSLRIDPATAAEWRERAAAAGLSVSDWIRGGVDAGQ
                     QTNLPTPRQRPVRVRDTSNDADPVLMRQLAGMSNNLNQIARALNECRLAGSPVQLVEVL
                     ALLRSIEAEAGRLLPQLPPPPKPTAKEASDAH"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          10..1409
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="80.514"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ggccggagga aatcagcgcg gtactggacg acttcgaagc gctggccttt gccggactgg
       61 atcgggagga cgtccacctg acagccgttc agcatgaaga accggatggc ggggtacatc
      121 tccacatcat gatcccccgc gttcacctgg gcacagggaa gtctttcaat ccagccccac
      181 caggtcaacg caaagacttc gacgccgtgc gcgacaaaca caaccacgaa aagggctggg
      241 cgcggccaga tgacccgctc agggcgcggt tgctgcggcc aaatttcgag gcgtacaagg
      301 gcaacaacga caagaccgcc atcaaaaaac agatcaccga tcacctgatc ggtgctgccg
      361 tgcagggtgt aatcaccaat gccgccgagc tgcgggaata cctacagaca tcgctcgggt
      421 gcgagatcac gcgcacggga gcggacttca tcagcatcaa gccggagggc tacccgaagg
      481 ccgtccggtt gaaaggggaa ctctatggag catcttggac cgcaaaaaac acgcttgagc
      541 gagaggctac agcagcagca agagcaggca ttgggcgagg tggagacgtt agcgaaagcg
      601 gtgctcgaag cgctcaagaa cgacttgcag cagcgtgtga acggcgagct ggatacaacc
      661 gtgaccgcta tcaggaaagg gatcgagggc ttgcccaagc agatcaccga ctcgtcccag
      721 ccgttgcagg cggcattgga gcaggcccag agcgaagcga acacgctcaa gctggtctcc
      781 agcgtctggc tcaaatccct gttgatcggg ctatgtaccg ccgtggggat cgggctgggg
      841 agttgggggg tgttgagctg gatgggcagc gaagtgcagc ggctgaacag cgagatcagc
      901 agcgcgaaag ccacgctggc gcagcttccc aagggggtga gctacgccaa gggcgaggat
      961 ggcaagggct acatcctcgc ccccagcatg gggacaccat tcgaaacgaa gagcggcaag
     1021 atggccgtgg aggtgaactg aatgaccgaa ctggagcgga tgctgctcga caggctggag
     1081 cggatagaga cggcgcaccg gcagcaaaca gcggctttgg agcagcaatt gcagcagcag
     1141 gcgcgctctt tgaacgagtt gcagaccgct tgcaccagcg cgctggcgag ctgcgggacg
     1201 ctatgcagcg agctacagca cgagttcgag acactgcgga acggcgtcga tcggtcgaac
     1261 agagcgacca cgacagcgct tggcagcttg agccgcagtg tcaacgactt gagcaaggcg
     1321 ctggacgcct tgcacagggc gcagcgttga ttgccgacaa ggctgtggag ctgcgaaaag
     1381 agcagcggct agatcatgga ttgagcatgt gatatgtcgt cactgtgaca ctaaatttgt
     1441 agattgaagt gcgattgaat gatcagtttc agggttaatg ctttggcaca ctggtcgctg
     1501 aaaattagcc ttcaacgtaa ggaacattaa atgagaatga aattgacggg gttgttatgt
     1561 tgcgtggcgt tgactggttg cgcaacagcc tatcagccct acagctattt cggcggcggt
     1621 gggtactctg acgtgcagct tgcagaaaat gtattccagg taaccgtaga agcaaatgga
     1681 tttacgacca gcaccgaggc cgctgacctt gctttgttgc gatcagctga cctaacgctt
     1741 gaaagaggat tcaaatattt cattatcggt tctacagctg atcattcgta ctcggcagga
     1801 tatacaacac cgtcaacgac cacattaaat gccactagct acggcaatac tatttacgga
     1861 acagcgcaaa caactggtgg gcaaacctat gtatttaatt tcccaacacc gtctatgaca
     1921 atcacctgtt tttctgatca accagcatta aatggaaccg tttatgatgc tgcaatcaca
     1981 tccagatccc tgcgagcaaa gctaggaata taaggtcggc cgtgctgcgc tagtcgcacc
     2041 cgttgcaggg gcgtgatacc atttaacacg tgctatagca cgtgttaatt tattggaggt
     2101 catcatggtc gcagcagcaa tgcgcagtaa gagaacggtc agcgtcacac tggagcccag
     2161 cttgctggaa cgagccagag cggcggggat caatctgtcc gccactctgg ctaccgcgct
     2221 acagcatgaa atcaatggct cagaggccga gcgctggaag cgtgagaaca gagccgcaat
     2281 acagactctt aaccggatgg tcgaagaacg cggcctgttt tctgacgatc acaggacgtt
     2341 ttgatgcagt tcacgatcta ccgcaacaag ggcaatgccc gcgactaccc ctatttgctg
     2401 aatgtgcaga gcgacatcat cggcgaactg cacacccgga tggtgatccc cttggtctcg
     2461 ctggcggact tccccggacg gcctgcacag cgacttaatc cggtgatcac cgtcgagggt
     2521 ggcaagtatc tggtgttaac ccacgaaatg gcggcggtgc gactggcgca gctgggggag
     2581 gaagtgaccg atgttcagga gtcgcgtcag ctcatcaagg gggcaataga tttcctgctg
     2641 gatggcgtgt aacgtactgt ccagaaaaga caaaccccga agctctgctg ttcaacttgg
     2701 cggaagactc agagcattcg gggctaggcg cgaacctatg gaaaaatgat accacatgga
     2761 tcacttgaga caaccccaag ccgccagtaa gcgcggtggt cattgacgaa tctagcccag
     2821 cttgatctgt tccgggagcg cctgccccgg aaaccctatt acaccgatga gctcaccacg
     2881 ggcctgcaca ttgcggatat tgcccgtgcc cttggctcac gctacatcca gccgaacggg
     2941 ccaacgcaca agcactggct cgtgtttgac gtagaccatt ccgccgcgac tctatcctgg
     3001 gacgacgtag gtgccccggc gcccaacatc acggccacga acagggataa cggccatgcc
     3061 catctgatct acgggctgga aatccctgtc cgcacagccc cggatggcaa ggtggccccg
     3121 ttgcgttatg ccgctgccat cgaggcggca cttcgggaac tgctgggggc ggatctgggc
     3181 tactccggtt tgatctgcaa gaaccccctg cacagccatt ggctggtgca agtctgggaa
     3241 cctcaaacct atacgctaga ttggttggct gattacctcg atttgagccc ctacaatggc
     3301 cgcaaacatc ttccggccta tggacttggg cgtaactgca ccctgttcga gaaaacgcgc
     3361 ttgtgggctt acaaggccat tcggcagggg tggccggggt tcgatacttg gcaggccgcc
     3421 gtgatcgagc gggcatccgg gtacaacgtc cagttcgagc agccgctccc ggccaacgag
     3481 gttaggcata cagccaagag catcgccaag tggacgcacc ggaacctaac tcccgcagga
     3541 ttcagcgccg tgcaggctgc cagaggggct aagggcgggc gcattggcgg caaggtatcg
     3601 aagggagggg gcaggcccag caaagcggca gagctgctgc cggaggttct acgactcaaa
     3661 gagatgggct acagcaaccg cgacattgcc gaggatctgc aaatatcggc aggtagcgtg
     3721 tccaattacc tccgccgtga tcatgagtga tcaaaaacaa agccttatca gataatagcc
     3781 cccgcagggg gttggttaag ccttaaaaga gtccctatag agagcggctt ggcgaggggg
     3841 gtaactgcga tcccgtaggg ctgtccatca ggccgtgtgt ttggcctgtg ggcagtagct
     3901 gttatccccg gagctgtttg gtctctgctg ttcgcagtgc ccgcaggatc tccccggctt
     3961 gctcctgtgt cagcaccacc gccgatcccc catccctgat cgcctgcatc tgggcagcca
     4021 gcccctccat cagcgcccta tttatcgtca cggtaacgtt attgtcttgc tgttttgccc
     4081 ggtatgccgc ttgtcgttct gccccggatt tggctgctcc ggtcttgggt ctaccccgcc
     4141 cccgcttggt gccgaacata tccccagtac cctgatcctt ggtgtccttc atggtgctat
     4201 ttcctgtcac tgtaacggta attgattatg tgtcactgtg acgataatta gcaagttgcc
     4261 gcccccctct ttttttgctg tactggtatt tagccagcct acccgtatta ctgtttcgcc
     4321 cgcttcgcgg actcccagta acacggggct ggtgaggggc gttgcccctt caatccccca
     4381 cacaccaccg ccggaggcat cgtgtccaaa gctccaagaa cgaccaccgt cagcctacgg
     4441 atcgatccgg ccactgccgc cgaatggcgc gagcgggctg ctgctgccgg gctcagtgtg
     4501 tcggactgga tcaggggggg ggtggatgca ggccagcaga cgaatttgcc cacgccccgt
     4561 cagcgccctg tccgtgtccg tgacaccagc aacgacgccg atccggtgtt gatgcgccaa
     4621 ttggccggga tgagcaacaa cctcaaccag attgcccgcg ccctgaacga gtgccggctg
     4681 gccggctcgc cggtgcaatt ggtggaggtg ctggccctgc tgcgcagcat cgaggcagag
     4741 gcaggccgct tgctgcccca gttgccgccg ccccccaaac cgaccgccaa ggaggccagc
     4801 gatgcacatt aaattcttgg ctcacggcac gggcagtgct gcccgtgcct cggcctacct
     4861 gatggggccg ctcgaccaca ccggggcaga acgggccggt gtgacggtcc tgcgggggga
     4921 tccggtgctg ttcgcggcgg tagctgactc gttgccgttc cagcatcgct attccagtgc
     4981 ggcgatctca tgggctgttg aagaagcgcc gc
//
