LOCUS       NZ_AP024140             6786 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Aeromonas caviae strain KAM329 plasmid pKAM329_4, complete sequence.
ACCESSION   NZ_AP024140
VERSION     NZ_AP024140.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00254948
            Assembly: GCF_015508545.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas caviae
  ORGANISM  Aeromonas caviae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Suzuki,M., Maehana,S. and Kitasato,H.
  TITLE     Whole genome sequencing of carbapenem-resistant Aeromonas spp.
            isolated in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   Suzuki,M., Maehana,S. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2020) Contact:Masato Suzuki National Institute of
            Infectious Diseases; 4-2-1 Aobacho, Higashimurayama, Tokyo 189-0002,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.4.8.0; Canu v. 1.8
            Genome Coverage                   :: 192x
            Sequencing Technology             :: Illumina MiniSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015508545.1-RS_2024_03_23
            Annotation Date                   :: 03/23/2024 01:47:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,922
            CDSs (total)                      :: 4,764
            Genes (coding)                    :: 4,613
            CDSs (with protein)               :: 4,613
            Genes (RNA)                       :: 158
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 122
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 38 of 151
            Pseudo Genes (incomplete)         :: 115 of 151
            Pseudo Genes (internal stop)      :: 22 of 151
            Pseudo Genes (multiple problems)  :: 20 of 151
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024140.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Aeromonas caviae"
                     /mol_type="genomic DNA"
                     /strain="KAM329"
                     /db_xref="taxon:648"
                     /plasmid="pKAM329_4"
                     /geo_loc_name="Japan:Kanagawa"
                     /collection_date="2018"
     gene            complement(join(6589..6786,1..1428))
                     /locus_tag="JN439_RS24385"
     CDS             complement(join(6589..6786,1..1428))
                     /locus_tag="JN439_RS24385"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="WP_202212053.1"
                     /translation="MLKDLSISKKLVLSFSLFFILLLSLGGMSFSLLNDAEEKFHIIST
                     QIIPDMLDSATLKQNLADVRRLELRFMLLGNADNLPQEEQLVAEVMTRAQKSLADYESN
                     LRTPEQRALFEEFLNKWQAYLPLHQEIMSLKREGQTEMAANKAVASIALYNGLIPLAGK
                     LMELNHQQASAMDAQFDRANKQAKRLIVGSLLAAALLVFGLAYWLSGQIRKPLAHLVEQ
                     ANRLSRGDLSGTLDPRCFARDELGQLASAFGSMHTHLRTLIADVAAAVHQLRTAVEEVN
                     AIATQSSQGVAQQLQEIDQLAAAINELQSTVQEVSRNCNEAANSANTAAGDSSAGLHAV
                     QLAIKDTGVVAQKLEQVGQVVDHLQQDSQSIGVVLEVIRSIADQTNLLALNAAIEAARA
                     GEHGRGFAVVADEVRTLAKRTQDSTSEINRIIDLLQSRARETVESMDHSRQLMDTTVNS
                     TSDVGSMIHKVTESVALISDMNSQIATATEEQNSVTEDLNRNIAGIHEAANEVASGAEQ
                     TASACLSLSQLAEQLQGRVAQFQL"
                     /name="methyl-accepting chemotaxis protein"
     gene            1831..2070
                     /locus_tag="JN439_RS24390"
                     /old_locus_tag="KAM329_47900"
     CDS             1831..2070
                     /locus_tag="JN439_RS24390"
                     /old_locus_tag="KAM329_47900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005740683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_016946643.1"
                     /translation="MSVMSVRLPDEVEQQLGQLAQSTGRTKSWLANQAIQDYLAREAWQ
                     ITEVEAALREADAGDFVPEKEMMAKFNRWGINAG"
                     /name="CopG family ribbon-helix-helix protein"
     gene            2060..2344
                     /locus_tag="JN439_RS24395"
                     /old_locus_tag="KAM329_47910"
     CDS             2060..2344
                     /locus_tag="JN439_RS24395"
                     /old_locus_tag="KAM329_47910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017411299.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_148726339.1"
                     /translation="MQVKWLRKAALNLEDAHDYLAKENPRVAQEFVFEVYRLVNLLVAQ
                     PAMGRPGRVPGTRELVLQPYPFLIPYRVNGEEIHILRVFHTRLRLPSHW"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2555..3499
                     /locus_tag="JN439_RS24400"
                     /old_locus_tag="KAM329_47930"
     CDS             2555..3499
                     /locus_tag="JN439_RS24400"
                     /old_locus_tag="KAM329_47930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_202212054.1"
                     /translation="MNRAQLDLFRERLPRKPYHTDEFTTGLSIADVSRALGARYIQPNG
                     PTHRHWLVFDVDHAAATLSWDDVGAPAPNITVTNRANGHAHLIYGLETPIRTAPDGKVA
                     PLRYAAAIEAALREKLGADLGYSGLICKNPLHTHWLVQVWEPASYDLSWLADYLDLSPY
                     NGRKQLPAYGLGRNCTLFEKTRQWAYKAIRQGWPGYEAWLSAVIDRAIGYNVQFEPPLP
                     ANEVRHIAKSIAKWTHRNLTPAGFSAVQTARGAKGGRLSKGGGRPSNSGKALADLLPEV
                     LRLKAQGYSNRDIAEDLQISAGSVSNYLRRDRE"
                     /name="replication initiation protein"
     gene            complement(3787..3936)
                     /locus_tag="JN439_RS24695"
     CDS             complement(3787..3936)
                     /locus_tag="JN439_RS24695"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223917703.1"
                     /translation="MTINLALVEGLDAQMQAIRDGGSAVVLTPEQAGEILRALRKAENK
                     QLRG"
                     /name="hypothetical protein"
     gene            4490..4690
                     /gene="mobC"
                     /locus_tag="JN439_RS24700"
     CDS             4490..4690
                     /gene="mobC"
                     /locus_tag="JN439_RS24700"
                     /inference="COORDINATES: protein motif:HMM:NF017524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_223917660.1"
                     /translation="MRQLAALGSNLNQVARALNECRSKGDRMPVLEALAYLRSIEAEAA
                     KLLPRLPPPPKPKAKGDDDAH"
                     /name="mobC"
     gene            4740..>5354
                     /locus_tag="JN439_RS24705"
                     /pseudo=""
     CDS             4740..>5354
                     /locus_tag="JN439_RS24705"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5392..5868
                     /locus_tag="JN439_RS24710"
                     /old_locus_tag="KAM329_47970"
     CDS             5392..5868
                     /locus_tag="JN439_RS24710"
                     /old_locus_tag="KAM329_47970"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223930722.1"
                     /translation="MEHLGQSKTLLSERIAQQKLAEQVEAERWTRELHDSVRSELIKLV
                     TGELDTTVRDMKAATAPLRSAMRQSRREVLLLRRFSGIWWKACLIGTCLSLGLGFGSWG
                     LLSWLGSEIQQRITTLEAIKGVEVIESNGKVYLAAPQLGTLINMKDGRKAVEVQ"
                     /name="hypothetical protein"
     gene            5869..6180
                     /locus_tag="JN439_RS24715"
                     /old_locus_tag="KAM329_47980"
     CDS             5869..6180
                     /locus_tag="JN439_RS24715"
                     /old_locus_tag="KAM329_47980"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_223930721.1"
                     /translation="MTELERILVGRLEQIETTHREQTAALTLQLQEQGRSLAEYQEYLN
                     GVLSDFETLCGGLQHDFGELLNAVETSEATTKITLGRLNTSVGSLMDALNTLLNARSK"
                     /name="mobilization protein"
     Typing          2563..3447
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.036"
                     /coverage="93.0"
                     /evalue="0.0"
ORIGIN
        1 gatcagcgcc accgattcgg tgaccttgtg gatcatggag cccacatccg aggtactgtt
       61 caccgtggtg tccatcaact gacggctatg atccatactc tccaccgtct cgcgggcccg
      121 gctctgcagc agatctatga tgcggttgat ctcgctggta gagtcctggg ttcgcttggc
      181 cagggtgcgc acctcgtcgg ccaccacggc aaaaccccga ccatgctcac cggcccgtgc
      241 cgcctcgatg gcggcattga gcgccagcag gttggtctga tcggcgatgg agcggatcac
      301 ctccagcacc acaccgatgc tctggctatc ctgctgcaga tgatccacca cctgcccgac
      361 ctgctccagc ttctgcgcca ccacaccggt atccttgatg gccaactgca cggcatgcaa
      421 ccccgccgag gagtcccccg ccgccgtatt ggccgagttg gccgcctcgt tacagttgcg
      481 cgacacctcc tgcaccgtgg attgcaactc gttgatggcg gcggccaact gatcgatctc
      541 ttggagctgc tgggcaaccc cctgagagga ttgggtagcg atggcattga cctcctccac
      601 cgccgttctc aattggtgaa cggcagcggc cacatcggca atcagggtcc gcagatgggt
      661 atgcatgctg ccaaaggccg aggccagctg acccagttcg tcgcgagcaa agcagcgcgg
      721 atccagcgtg ccgctcaaat cgcctctgga gagccgattg gcctgctcca ccaggtgcgc
      781 cagcggcttt ctgatctggc cggagagcca gtaggccagc ccaaacacca gcaacgccgc
      841 cgccagtagc gaaccgacga tcaagcgctt ggcctgttta ttggcacggt caaactgggc
      901 atccatggca ctagcctgct gatggttcag ttccatcagc ttgccagcca gggggataag
      961 cccattgtag agcgcaatgg atgccaccgc cttattggcc gccatctcgg tctgcccttc
     1021 tcgcttcagg ctcatgatct cctggtgcag gggcagataa gcctgccact tgttgaggaa
     1081 ctcctcgaac agcgcccgct gctccggggt gcgcaaatta ctctcataat ccgccaggct
     1141 cttctgcgcc cgggtcatca cctcggccac caactgctcc tcctgaggta gattgtccgc
     1201 attgcccagc aacatgaagc gcagctccaa gcgccggaca tccgccaggt tttgcttgag
     1261 tgtcgcacta tcgagcatat ccggtatgat ctgggtactg atgatatgaa acttctcttc
     1321 ggcatcgttt agcaggctga acgacatgcc ccccagcgac agcaacagga taaaaaagag
     1381 agaaaacgac aacaccagtt ttttactgat tgatagatct tttaacattc caacgctcca
     1441 tcattgttta taaaaaatcc tgattaccat catgattcag cccctcatga ctatacttag
     1501 ggagggtgcg aataagcacc ttccacaacg aaactgaaaa aattagttta ttaaattcaa
     1561 atacttacaa aggcgcactt ttatgtttgg tgacttattc gcaccttcct tagagtattc
     1621 tgttctggag ggtttaatca tgaccaagaa cagggttcag tttcaaaaag catgaattac
     1681 aactcaacgc ttacaaagtc ggtgcgcata aggggcgtta cggtaaatcc gccgcccctt
     1741 tttgctgcct ggcgtcagcg atcggggact gtcgccatgc cgtagtcttg gtgtagtatt
     1801 gtgtaaacat tgtagtttaa gggggtgggt atgagtgtta tgtctgtccg tttgccggat
     1861 gaggtagagc agcagttggg gcaattggcc cagtccacag gcagaaccaa atcatggttg
     1921 gcaaaccaag ctatccagga ttatttggcg cgagaagcct ggcagatcac ggaagttgaa
     1981 gccgcattgc gtgaagcgga tgcgggcgac tttgtgcctg aaaaggagat gatggcgaag
     2041 ttcaatcggt ggggtatcaa tgcaggttaa atggctgcgc aaggctgcac tgaatctgga
     2101 ggatgcccac gattacctgg cgaaagagaa ccctagggtt gcccaagagt ttgtgttcga
     2161 ggtgtatcgc ttggtcaatc tgttggtagc tcagcccgct atgggtcgtc ctggccgcgt
     2221 accaggaaca agagagcttg tgctgcaacc ctacccgttc ctcattccat accgagtgaa
     2281 tggcgaggag atccacattc tgagggtgtt tcatacccgt ttgcggctac caagccactg
     2341 gtaaaggata cttttaggtg gggtggtatg cgtttgcgta gggaggggat cgtgcggtaa
     2401 cgtactgcct agaaaagaca aaccccgaat ctctgttcat cgacttggcg gaagaacaac
     2461 agaggattcg gggccgtact tatgcaatag gtgaaatata tcacatggat cacaccagac
     2521 aaccctttag cgccgccaat atgcgcggtg gtcattgaat cgagcacagc ttgacctgtt
     2581 ccgggagcgc ctgccccgga agccctacca caccgacgaa tttaccacgg gtctgtccat
     2641 tgcggatgtc agccgtgccc ttggggctcg ttacatccag ccgaacgggc caacgcatcg
     2701 gcattggctc gtgtttgacg tagaccatgc tgccgctacg ttatcctggg atgacgtagg
     2761 cgccccggca ccgaacatca cggtgacgaa cagggccaac ggccacgccc acctgatcta
     2821 cggactggag accccgatcc gcactgcccc ggacggcaag gtggccccac tgcggtatgc
     2881 cgccgccatc gaggccgccc tccgggagaa actgggggcg gatctgggct actccgggct
     2941 gatctgcaag aaccccctgc atacccactg gcttgtccag gtatgggagc ccgcctccta
     3001 cgatctgagc tggctggccg actacctcga tttaagcccc tacaatggcc gcaaacagct
     3061 tccagcttac gggttggggc gtaactgcac cttgttcgag aaaacgcgcc agtgggctta
     3121 caaggcgatt aggcaggggt ggccggggta tgaggcttgg ctctctgccg ttatagatcg
     3181 ggcgatcggg tacaacgtcc agttcgagcc gccgctcccg gccaacgagg tcaggcacat
     3241 cgctaagtcc attgcgaagt ggacgcaccg caacctaacc cccgcagggt tcagcgccgt
     3301 acagaccgcc agaggggcca agggaggcag gctatctaag gggggcggta ggcccagcaa
     3361 tagcggtaaa gccctcgcag acctgctgcc ggaggtgcta cggctcaagg ctcagggtta
     3421 cagcaaccgt gacattgccg aggatctgca aatttcggcg ggcagtgtgt ccaattacct
     3481 tcgccgtgat cgtgagtgat caaaacaagc cttatcagat agtagccccc gcagggggtt
     3541 ccttgagccc ttaagcgaat ctgagtgatc aaaacaagcc ttatcagata gtagcccccg
     3601 cagggggttc cttgagccct taagcgaatc tgagtgatca aaacaagcct tatcagatag
     3661 tagcccccga agggggttct ttgagccctt aaaatcattc aagagagagg attggtgcga
     3721 ggggggtaag gccgatcccg aagggctgtc catcaggcca tgtgtttggc ctgtgggcag
     3781 taggtcttat ccccggagct gtttgttctc tgcctttcgc agtgcccgca ggatctcccc
     3841 ggcctgctcc ggggtcagta ccaccgccga ccctccatcc cggatagcct gcatctgggc
     3901 atccagcccc tctaccaatg ccagatttat cgtcacagta acgttattgt cctgctgctt
     3961 tgcacggtag gctgcttgtc gttctgcccc tgttttggct gatccggttt ttggcctacc
     4021 ccgtccgcgc ttgatgccaa acatgtcccc agtcacctga tccttggtgt ccttcatggt
     4081 gttatttcct gtcactgtaa cggtaattga ttatgtgtca ctgtgacgat aattggcaag
     4141 ttgccgaccc cctgtttttt tgctgtactg gcattaagcc agcctacccg tattacgttg
     4201 ctgcgcaact acatacgggg ctgttgaggg gcgctgcccc ttcaatcccc caccccaaaa
     4261 ccacaccgga ggcaccgtgg ccaaagtccc gagaagcacg accgtgagcg tccgaataga
     4321 ccaggccacg gcggaggtat ggcgccagca ggcgacctct gctggcctca gtgtatcgga
     4381 ctggatcagg ggggcggtcg atgctgggca gcagacgcac atccccacgc cccgcaagcg
     4441 cccctcacgc cccccacggg ataccagcaa cgatgctgac cccgctttga tgcggcaact
     4501 ggccgcgctt ggctcgaacc tgaatcaggt cgctcgggca ctcaacgagt gccggagtaa
     4561 gggtgatcgg atgccggtgt tagaggcgct ggcctatctg cggagcatcg aggccgaggc
     4621 ggccaagttg ctcccccggc taccgccccc acccaaacca aaggcaaagg gggacgacga
     4681 tgcacattaa gtttatggcc cacggcacag gctctggcgc tgctgccagt gcctacctga
     4741 tgggggatct agaccacaag ggggttgctc gggcaggcgt tgaggtgctg cggggagacc
     4801 cggtgttgtt cggtgccttg gccgactctc tgccgttcca gcagcggtat accagcgccg
     4861 ttatctcttg ggctaccgaa gaggcaccga cagaggatga aatcaacgcc gtgctggacg
     4921 acttcgagaa gttggctttt gccggactgg acaggcagga cgtacacctg accgccgtcc
     4981 tgcaccgcga ggaagatggc ggtgtgcatg tccacatcct gatcccccgc gttcacctgg
     5041 gcacgggaaa atccctgaac gtagccccgc caggtcaacg gagccacttc gattcgctgc
     5101 ggaacaaatg gaactacgga aagggctggg cacggccaga cgacccgctc aggcggcggc
     5161 ctgtgcagcc cagctttgac gcctacaggg acgccaacga ccgcaaggca atcaagaaac
     5221 aggtctcgga gtacctgctg gggttcatcg cccaaggggt gatcaccaac gccgctgatc
     5281 tgcggcgaaa ggtcgtggaa atgggatgcg aaatcacgcg atccggtgcc gatcatctca
     5341 gtatcaagcc agagggatac ccgaaggcaa tcagactcaa aggggagatt tatggagcat
     5401 cttggacagt cgaaaacact cttgagcgag aggatcgcgc agcagaagct cgccgaacag
     5461 gtagaggcgg aacggtggac aagggagctg cacgattctg tcaggagcga attgataaag
     5521 cttgtgacag gcgagctgga tacaaccgtc agagatatga aggccgcgac cgcaccgctg
     5581 agatcggcca tgaggcagtc caggagggag gtcctgttgc tgaggcgatt ttccggcatt
     5641 tggtggaaag cgtgtctgat cggcacgtgc ctgtcattgg ggctcgggtt cgggagctgg
     5701 gggttgttga gctggctcgg cagcgagatc cagcagcgga tcacgacgct cgaagccatc
     5761 aagggggtgg aggtcatcga gagcaacggc aaggtgtacc tagcagcccc ccaactcggg
     5821 actttgatca acatgaaaga cggtcgcaaa gccgtggagg tgcagtgaat gacggaactg
     5881 gaacggatct tggtcggcag gctggagcaa atcgagacga cacacagaga gcagaccgcg
     5941 gccttgacgc tgcaattaca ggagcagggg cgctctttgg ccgagtatca ggagtactta
     6001 aacggggtac tcagcgactt cgagacgctg tgcgggggtc tacagcacga cttcggggag
     6061 ctgttgaacg ccgtggagac atcagaggcc acgaccaaga taacgctagg tcgattgaac
     6121 accagtgtcg ggagcttgat ggacgcgctg aataccttgc tcaatgcgcg gagcaagtag
     6181 cggcactggc gaaagtggag aaggcgaagg ggctaaggat tggggggctt ggcatgtaaa
     6241 tctggtcgaa accgaacccc gcaagatccg gccctcaaac ggccctcaat gcgtcgcaaa
     6301 ctttcaggcc ataaacgggt caggcagggg gaagaaacgc gccagaatgc ggctcaggcg
     6361 gtcgattttc ggcagggtgt cgattttgcc aggtgaagtc ggctattaaa cataatgggc
     6421 attaggcgca ttatgtttct ggttggtgtg gcgcaataat tctcatggtg agaggtcaat
     6481 acagagcgtg aatctctgtt ctccaatggt ttcagttaca aggtcacata acctcggcaa
     6541 gtttcctgcc gccgaatggc ggcaggcgct ggatacaata aaccacagtt acagctggaa
     6601 ctgggcaacc cgtccctgca gctgttcggc caactggctc agggagaggc aggcggaggc
     6661 cgtctgctcg gcgccactgg ccacctcgtt ggccgcctca tggatccccg ctatgttgcg
     6721 gttcagatcc tcggtcaccg agttctgctc ctcggtggcg gtggcaatct ggctgttcat
     6781 gtccga
//
