LOCUS       NZ_AP024129             6668 bp    DNA     circular CON 20-SEP-2023
DEFINITION  Escherichia coli strain NS-NP030 plasmid NS-NP030_P3, complete
            sequence.
ACCESSION   NZ_AP024129
VERSION     NZ_AP024129.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00077547
            Sequence Read Archive: DRR103139, DRR252637
            Assembly: GCF_005393605.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Frederic,A., Alexandre,P., Arimizu,Y., Camille,V.C., Nadege,G.,
            Clemence,M., Hubert,B., Jean-Philippe,N., Hayashi,T., Priscilla,B.,
            Ogura,Y. and Eric,O.
  TITLE     Insights into the acquisition of the pks island and production of
            colibactin in the Escherichia coli population
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Hayashi,T., Ogura,Y. and Katsura,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) Contact:Yoshitoshi Ogura Kurume University
            School of Medicine, Department of Bacteriology; 67 asahi-machi,
            Kurume, Fukuoka 830-0011, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Coverage                   :: 54x
            Sequencing Technology             :: Illumina Miseq, MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/20/2023 05:26:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,403
            CDSs (total)                      :: 5,276
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 97 of 223
            Pseudo Genes (incomplete)         :: 132 of 223
            Pseudo Genes (internal stop)      :: 62 of 223
            Pseudo Genes (multiple problems)  :: 60 of 223
            Pseudo Genes (short protein)      :: 4 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024129.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="NS-NP030"
                     /isolation_source="feces"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /plasmid="NS-NP030_P3"
                     /geo_loc_name="Japan"
                     /collection_date="2014"
     gene            complement(331..522)
                     /locus_tag="FFJ14_RS26765"
                     /old_locus_tag="BvCmsNSNP030_5274"
     CDS             complement(331..522)
                     /locus_tag="FFJ14_RS26765"
                     /old_locus_tag="BvCmsNSNP030_5274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            586..909
                     /locus_tag="FFJ14_RS26770"
                     /old_locus_tag="BvCmsNSNP030_5275"
     CDS             586..909
                     /locus_tag="FFJ14_RS26770"
                     /old_locus_tag="BvCmsNSNP030_5275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1118..>1549
                     /locus_tag="FFJ14_RS27150"
                     /pseudo=""
     CDS             1118..>1549
                     /locus_tag="FFJ14_RS27150"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1587..2105
                     /locus_tag="FFJ14_RS26780"
                     /old_locus_tag="BvCmsNSNP030_5276"
     CDS             1587..2105
                     /locus_tag="FFJ14_RS26780"
                     /old_locus_tag="BvCmsNSNP030_5276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            2112..2366
                     /locus_tag="FFJ14_RS26785"
                     /old_locus_tag="BvCmsNSNP030_5277"
     CDS             2112..2366
                     /locus_tag="FFJ14_RS26785"
                     /old_locus_tag="BvCmsNSNP030_5277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015059032.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2684..3103)
                     /locus_tag="FFJ14_RS26790"
                     /old_locus_tag="BvCmsNSNP030_5278"
     CDS             complement(2684..3103)
                     /locus_tag="FFJ14_RS26790"
                     /old_locus_tag="BvCmsNSNP030_5278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(3184..3621)
                     /locus_tag="FFJ14_RS26795"
                     /old_locus_tag="BvCmsNSNP030_5279"
     CDS             complement(3184..3621)
                     /locus_tag="FFJ14_RS26795"
                     /old_locus_tag="BvCmsNSNP030_5279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            3881..5449
                     /gene="cea"
                     /locus_tag="FFJ14_RS26800"
                     /old_locus_tag="BvCmsNSNP030_5280"
     CDS             3881..5449
                     /gene="cea"
                     /locus_tag="FFJ14_RS26800"
                     /old_locus_tag="BvCmsNSNP030_5280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(5446..5787)
                     /locus_tag="FFJ14_RS26805"
                     /old_locus_tag="BvCmsNSNP030_5281"
     CDS             complement(5446..5787)
                     /locus_tag="FFJ14_RS26805"
                     /old_locus_tag="BvCmsNSNP030_5281"
                     /inference="COORDINATES: protein motif:HMM:NF015489.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_000058760.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            5835..5972
                     /locus_tag="FFJ14_RS26810"
     CDS             5835..5972
                     /locus_tag="FFJ14_RS26810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(6171..6329)
                     /locus_tag="FFJ14_RS27300"
     CDS             complement(6171..6329)
                     /locus_tag="FFJ14_RS27300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     Typing          159..246
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(6258..6667)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.594"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          900..2470
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.809"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgttaccgt ttttgtgtga gtcagtaccg ctcgccgcag tcgaacgacc gagcgtagcg
       61 agtcagtgag cgaggaagcg gaaaagcgcc tggacgtgca ttttctcctt acgcatctgt
      121 gcggcatttc acacccggca tggcgtactt ttcatacaat ccgcactgat gccgcatggt
      181 taagccagta tacactccgc tatcgctacg tgactgggtc agggctgcgc cccgacaccc
      241 gctaaaacct gctgacgcgc cctgacgggc ttgtcagctc ccggcatccg ctcacagaca
      301 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat ccccgaaacg
      361 tgcgaggcag ctgcggtaaa gctcatcggc gtggtcgtga agcgattcac aaatatcggc
      421 ctgttcatct gcgtccagtt cgttgagctt ctccagcagc gttaatgtct ggcttctgat
      481 aaagcgggcc atgttaaggg cggttttttc ctgtttagtc actgatgcct ccgtgtaagg
      541 gggatttctg ttcatggggt aatgataccg atgaaacgcg agaggatgct cacaatacgg
      601 gttactgatg atgaacatgc ccggttactt gaacgctgtg agggtaaaca actggcggta
      661 tggatgcggc gggtctgcct gggggagccg gttgcccgtt ccggaaaact gccgacactg
      721 gcaccgccgt tactgcgtca gctggccgcc atcggaaata acctgaatca gacagcccgt
      781 aaggtgaaca gcgggcagtg gtcttccggt gaccgggttc aggtggtggc cgcactgatg
      841 gccatcgggg atgagctgcg ccggctgcgt ctggctgtca gggaacaggg ggcgcgggat
      901 gatagttaaa tttcatgcca ggggaaaagg tggtggcagt ggtccggttg attacctgct
      961 ggggagggag cgtaaccgcg aaggcgcaac ggtgcttcag ggtaatccgg aagaagtccg
     1021 ggaactcatc gatgccacgc catttgcgaa gaaatacacg tccggtgttc tgtcgttcgc
     1081 ggagaaggag ctgccgccgg gaggacgtga aaaagtgatg gcgagctttg agcgtgtact
     1141 gatgcccggt ctcgaaaaaa atcagtacag catcctgtgg gtggagcacc aggacaaggg
     1201 acggcttgag ctgaattttg tcattccgaa catggagcta cagaccggaa aacgcctcca
     1261 gccgtactac gaccgcgcag acaggcctag aattgatgcc tggcagacgc tggtgaatca
     1321 ccattacggg ctgcatgacc cgaacgcccc ggagaaccgc aggacgttga cactccctga
     1381 taacctgcct gaaacgaaac aggcgcttgc tgagggcgtc acgcgaggta tagatgcact
     1441 ttaccatgcc ggagagataa aaggccgtca ggatgtgatt caggcgctca ctgaggcggg
     1501 gctggaagtg gtcagggtga cgcgaagcag tatcagcatt gcagatccga acggcgggaa
     1561 gaatatcagg ctgaaaggag cattttatga gcaatctttt gcagacgggc gcggagttcg
     1621 agaaaaagct gaaagagaga gccgaatcta cagagaaaat gctgaacaac gagttcagga
     1681 ggctcggcga atctgtaagc gaggctgtga catcaaacga gacgaaaatc agagacgcta
     1741 tagccctgtt cacagcctcg acagaggaat cgctggaaaa acaccgggaa ggggtgaaag
     1801 aggcgatgat gcagcacagg agggacgtgt taaagctggc agggaatacg ggcatgatgt
     1861 tactggggat agtctttctc ctgtttaccg cgagtggcgg gacgctctgg tatcttggag
     1921 ggaggataca ggcgaacctg gaagaaatca ggaagcagga agagacattg cagaaactga
     1981 acgcgaagac atggggcgtg gagtttgtgc aggacgggaa caggaaattc cttgtccttc
     2041 cgtacgggaa atcagcggag gtgattccct ttcaggggaa agagtgggta catctgaagg
     2101 agtgacacag agtgacagag ctggaaacac atttgctgaa cgccttagag cagctgcaac
     2161 aggactatat gcagcggctg agcgaatggg agagcgcctt cgtggaattg cagaagatgt
     2221 tttcgcttac gcaacgggac aacgcgatgc tgaacgagcg ggtcatgcag ttgagtcagc
     2281 aggtgcagca cttgagcgag cagacagaac gcttgagcga gcagacagaa cgcttgagcc
     2341 agttatacag cgagaactgg agataagaga ggaacggctg atacaggagc gcgaacatgt
     2401 gttatccctg gaacgggagc gtcagccgga aatacaggaa cgcacgctgg atggcccttc
     2461 gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg attaagtggg ggtaatgtgg
     2521 cctgtaccct ctggttgcat aggtattcat acggttaaaa tttatcaggc gcgatcgcgg
     2581 cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc gcgctgaacg
     2641 cgttttagcg gtgcgtacaa ttaagggatt atggtaaatc cacttactgt ctgccctcgt
     2701 agccatcgag ataaaccgca gaaatcgtgt cagccagcag cctggatttt ttcggggtaa
     2761 ggtctttgct gcggtaatca gtacccgcaa attgagttgt gccccggagt gagtttaaaa
     2821 attcctggct gtgaaactgc tgaatgcgtt gttgcacatc actccgtgat tttcctgtcg
     2881 ctttatcttt agctcccata tcccagaact gccgaaaaaa aggcaccatc attcgtgttg
     2941 tgtcggcttt ttgggtggaa aagttgggat caaaaccgtc atcagaagca tatacgtaat
     3001 gccgggtatg ttgttccacg ctgccctttg gggtacaggc tgtgagagct actgttgctg
     3061 ttatcatggc gataagacaa agtttgtttc cggtacgtct catgagtgtc tccctacctt
     3121 aaagtattta ttctcggtga gttattatcc gaagactatt ttatcatcag tctctgacag
     3181 cgttcagcga ccgaacaacc tggaccagaa tccccgtttc ttttccagtt ccagtgcctg
     3241 cctgagctgg gctatctcat tttgtagctg ttcccgttct gcttcctggc gtctgcgatc
     3301 catatcctgt gcctgtttat cctcaagcat cagcgtcagg cattgtttca gctcattcag
     3361 ctcccggaga atgcgttctg tctgctgatc atgtggattt tctgcatgtc cctcactgtg
     3421 cctttctggt gtctcattct gctttaattc gccgtatgcc cggatcagtt cactggtttc
     3481 aaactcccgt cgtccgtccc tgccggtacg gtaactgaca aggcctgcgc gcatatcacg
     3541 gtaaaactgt gaacgcgatc tgcctgtcat ttttagtgcg tcccggagtg tgtgccatgc
     3601 cataaagtga cagtgtccca tagatgtctc atctcatagt ttcagtaaaa cataatgagg
     3661 tctgagaacg gtaatgtttg tgctggtttt tgtggcatcg ggcgagaata gcgcgtggtg
     3721 tgaaagactg tttttttgat cgttttcaca aaaatggaag tccacagtct tgacagggaa
     3781 aatgcagcgg cgtagctttt atgctgtata taaaaccagt ggttatatgt acagtattta
     3841 tttttaactt attgttttaa aagtcaaaga ggattttata atggaaaccg cggtagcgta
     3901 ctataaagat ggtgttcctt atgatgataa gggacaggta attattactc ttttgaatgg
     3961 tactcctgac gggagtggct ctggcggcgg aggtggaaaa ggaggcagta aaagtgaaag
     4021 ttctgcagct attcatgcaa ctgctaaatg gtctactgct caattaaaga aaacacaggc
     4081 agagcaggct gcccgggcaa aagctgcagc ggaagcacag gcgaaagcaa aggcaaacag
     4141 ggatgcgctg actcagcgcc tgaaggatat cgtgaatgag gctcttcgtc acaatgcctc
     4201 acgtacgcct tcagcaacag agcttgctca tgctaataat gcagctatgc aggcggaagc
     4261 agagcgtttg cgccttgcga aagcagaaga aaaagcccgt aaagaagcgg aagcagcaga
     4321 aaaggctttt caggaagcag aacaacgacg taaagagatt gaacgggaga aggctgaaac
     4381 agaacgccag ttgaaactgg ctgaagctga agagaaacga ctggctgcat tgagtgaaga
     4441 agctaaagct gttgagatcg cccaaaaaaa actttctgct gcacaatctg aagtggtgaa
     4501 aatggatgga gagattaaga ctctcaattc tcgtttaagc tccagtatcc atgcccgtga
     4561 tgcagaaatg aaaacgctcg ctggaaaacg aaatgaactg gctcaggcat ccgctaaata
     4621 taaagaactg gatgagctgg tcaaaaaact atcaccaaga gccaatgatc cgcttcagaa
     4681 ccgtcctttt tttgaagcaa ccagacgacg ggttggggcc ggtaagatta gagaagaaaa
     4741 acaaaaacag gtaacagcat cagaaacacg tattaaccgg ataaatgctg atataactca
     4801 gatccagaag gctatttctc aggtcagtaa taatcgtaat gccggtatcg ctcgtgttca
     4861 tgaagctgaa gaaaatttga aaaaagcaca gaataatctc cttaattcac agattaagga
     4921 tgctgttgat gcaacagtta gcttttatca aacgctgact gaaaaatatg gtgaaaaata
     4981 ttcgaaaatg gcacaggaac ttgctgataa gtctaaaggt aagaaaatcg gcaatgtgaa
     5041 tgaagctctc gctgcttttg aaaaatacaa ggatgtttta aataagaaat tcagcaaagc
     5101 cgatcgtgat gctattttta atgcgttggc atcggtgaag tatgatgact gggctaaaca
     5161 tttagatcag tttgccaagt acttgaagat tacggggcat gtttcttttg gatatgatgt
     5221 ggtatctgat atcctaaaaa ttaaggatac aggtgactgg aagccactat ttcttacatt
     5281 agagaagaaa gctgcagatg caggggtgag ttatgttgtt gctttacttt ttagcttgct
     5341 tgctggaact acattaggta tttggggtat tgctattgtt acaggaattc tatgctccta
     5401 tattgataag aataaactta atactataaa tgaggtgtta gggatttaat tattctttat
     5461 tgatataaaa agtcctagca atccaaatgg gattgctagg accaaacaaa gtagattata
     5521 tagcataaat aggtttaatt ttgctacggg ggcgttattt aggttttttc ttctttcgaa
     5581 aaaatctttc tttatgaagt taaaagctat gtattcaata gcatattttg aatatggaca
     5641 tagaatagtg cttatcacta ttgcatatag catcttatct gacacaagga aataataccc
     5701 ttcgctgttt tttgttataa ggtatatata tataagtgtg cagtacaggc caaataaaat
     5761 attttttatg tagtatctta agctcataaa ttaaacctcg ccatatattc ttttcatttt
     5821 ataaggatcg agttatgagg aaaagatttt ttgtgggaat attcgcgata aacctccttg
     5881 ttggatgtca ggctaactat atacgtgatg ttcagggagg gaccatcgca ccatcctcct
     5941 cttctaaact gacggggatc gcggttcagt agaaaagatc aaaggatctt cttgagatcc
     6001 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac caacggtggt
     6061 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6121 gcagatacca aatactgtcc ttctagtgta gccgtagtcg ggccactact tcaagaactc
     6181 tgtagcaccg tttgtgccat catcgctctg ctaatccggt taccagtggc tgctgccagt
     6241 ggcgttaagg cgtgccttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6301 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6361 gaactgagat accaacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6421 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6481 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6541 ctatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgcctg ctacgtggcc
     6601 ttcttcctgt tcctggtctt ttgctcacat gttctttccg gccttatccc ctgattctgt
     6661 ggataact
//
