LOCUS       NZ_AP024118             6715 bp    DNA     circular CON 20-SEP-2023
DEFINITION  Escherichia coli strain JML285 plasmid JML285_P4, complete sequence.
ACCESSION   NZ_AP024118
VERSION     NZ_AP024118.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00077348
            Sequence Read Archive: DRR102940, DRR252635
            Assembly: GCF_005389605.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Frederic,A., Alexandre,P., Arimizu,Y., Camille,V.C., Nadege,G.,
            Clemence,M., Hubert,B., Jean-Philippe,N., Hayashi,T., Priscilla,B.,
            Ogura,Y. and Eric,O.
  TITLE     Insights into the acquisition of the pks island and production of
            colibactin in the Escherichia coli population
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   Hayashi,T., Ogura,Y. and Katsura,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) Contact:Yoshitoshi Ogura Kurume University
            School of Medicine, Department of Bacteriology; 67 asahi-machi,
            Kurume, Fukuoka 830-0011, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Coverage                   :: 40x
            Sequencing Technology             :: Illumina Miseq, MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/20/2023 05:13:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 4,867
            CDSs (with protein)               :: 4,867
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 217
            CDSs (without protein)            :: 217
            Pseudo Genes (ambiguous residues) :: 0 of 217
            Pseudo Genes (frameshifted)       :: 91 of 217
            Pseudo Genes (incomplete)         :: 131 of 217
            Pseudo Genes (internal stop)      :: 49 of 217
            Pseudo Genes (multiple problems)  :: 48 of 217
            Pseudo Genes (short protein)      :: 3 of 217
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024118.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="JML285"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="JML285_P4"
                     /geo_loc_name="Japan"
                     /collection_date="2015"
     gene            join(6506..6715,1..24)
                     /locus_tag="FFF71_RS25675"
     CDS             join(6506..6715,1..24)
                     /locus_tag="FFF71_RS25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001448503.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(399..605)
                     /locus_tag="FFF71_RS25680"
                     /old_locus_tag="HmCmsJML285_5061"
     CDS             complement(399..605)
                     /locus_tag="FFF71_RS25680"
                     /old_locus_tag="HmCmsJML285_5061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096539.1"
                     /translation="MAKLPQEKPKEVIQAGFDDVKKAYKKGAKTVNDTYAEQAGRDLQS
                     GDGKLMATYRRNKKAIDKLDAEG"
                     /name="hypothetical protein"
     gene            complement(598..981)
                     /locus_tag="FFF71_RS25685"
                     /old_locus_tag="HmCmsJML285_5062"
     CDS             complement(598..981)
                     /locus_tag="FFF71_RS25685"
                     /old_locus_tag="HmCmsJML285_5062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000065853.1"
                     /translation="MSNIVHLSDKKQKNKPVDISFDDYTDNLSKGFVKNLSNKQKKELI
                     SKIKAILKSRNVAFRSTPPSDWKEKSRTFAIFFPEKKIVFTLGVVNKLKIEDGWINHKI
                     SDTEDAVLSVGKIINLIEDSQHG"
                     /name="hypothetical protein"
     gene            complement(1372..2433)
                     /locus_tag="FFF71_RS25690"
                     /old_locus_tag="HmCmsJML285_5063"
     CDS             complement(1372..2433)
                     /locus_tag="FFF71_RS25690"
                     /old_locus_tag="HmCmsJML285_5063"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034299.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HindVP family restriction endonuclease"
                     /protein_id="WP_000034299.1"
                     /translation="MSKIESLYGLKNTNRKESDLWGKNQFNSSFPTSLACYMRDNKMNP
                     IYLYLDKDLKVHAKEVSFDFVFNTTLKNSDLEFQFEKKYEPYQQYAYDDIKGIDLVIKG
                     NNSFLKALEIKLTVLPDSTTYKENESEWSSEIVIRPASTSYCALGIMHSCSDKFHEIRK
                     IFESTCESIQHWGNETEVLSKKENILNCLNTFQENFLDYQKPFLMQPLWKTKGKSPILD
                     QNAFDIFIWSDFALCRTFIDRSSYNNSSASRLMRSSARLARMVYEIASKNKTNINRIYT
                     EMNFGYQSDKEFSLNGKITKAYMDHPRRTKPILPPSVLKEIILNGGQNKLSPERRFDQS
                     VYYTASHLFDEIK"
                     /name="HindVP family restriction endonuclease"
     gene            2521..3678
                     /locus_tag="FFF71_RS25695"
                     /old_locus_tag="HmCmsJML285_5064"
     CDS             2521..3678
                     /locus_tag="FFF71_RS25695"
                     /old_locus_tag="HmCmsJML285_5064"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:1904047 -
                     S-adenosyl-L-methionine binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001219937.1"
                     /translation="MRIIDLFSGCGGLSLGFLKGGFDVVGAYDFWDPAIECYRDNFSHP
                     IKKLDLSNVDDVVRELKDIDFDMIIGGPPCQDFSHAGLRIEGARANLTRSFSEIIKRIK
                     PKWFVMENVDRALRSGAYLEARGIFKESGYGLTEIVLDASKCGVPQKRKRLFVIGKLDV
                     RDGFILNEVMCGISKDSMTVRNYLGDSLGIEYYYRHPRNYNRRAIFSIDEPAPTVRGVN
                     RPIPDGYLGHAGDPVSISENVRPLTTFERARLQTFPEDFKFKGAKTNLEQMIGNAVPVE
                     LAKYVAVTIMEYEKKQVKGIYDKEGFRAWLLNEKKLTKRTSSDIISRCCRGVSFFDSEG
                     VDFYNCEIDEIIMKLERLESFVRLGVSLKSQLRRAFKLYYEYCRR"
                     /name="DNA cytosine methyltransferase"
     gene            complement(3906..4064)
                     /locus_tag="FFF71_RS26270"
     CDS             complement(3906..4064)
                     /locus_tag="FFF71_RS26270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001557254.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHDLTPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="hypothetical protein"
     gene            complement(4724..4915)
                     /locus_tag="FFF71_RS25700"
                     /old_locus_tag="HmCmsJML285_5065"
     CDS             complement(4724..4915)
                     /locus_tag="FFF71_RS25700"
                     /old_locus_tag="HmCmsJML285_5065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165984.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGEDL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4980..5303
                     /locus_tag="FFF71_RS25705"
                     /old_locus_tag="HmCmsJML285_5066"
     CDS             4980..5303
                     /locus_tag="FFF71_RS25705"
                     /old_locus_tag="HmCmsJML285_5066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955994.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIEGELRSLRQVVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5512..>5943
                     /locus_tag="FFF71_RS26065"
                     /pseudo=""
     CDS             5512..>5943
                     /locus_tag="FFF71_RS26065"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5981..6499
                     /locus_tag="FFF71_RS25715"
                     /old_locus_tag="HmCmsJML285_5067"
     CDS             5981..6499
                     /locus_tag="FFF71_RS25715"
                     /old_locus_tag="HmCmsJML285_5067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001447511.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGMSAEVTHYQ
                     GKDWIQLTE"
                     /name="MbeB family mobilization protein"
     Typing          4562..4646
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="100.0"
                     /evalue="6.569999999999999e-34"
     Typing          complement(3993..4542)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.298"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4948..5297
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.162"
                     /coverage="99.0"
                     /evalue="1.96e-64"
     Typing          5294..6711
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.658"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 ttatacagcg agaactggag ataagagagg aacggctgat acaggagcgc gaacatgcgt
       61 tatccctgga acgggagcgt cagccggaaa tacaggaacg catactggat ggtccttcgt
      121 tgtcgctgtg atggcgaaac tataaaaaat ggcaggttca gtggattttg acggactaat
      181 gtagtctgca ccatctggtt gcataggtat tcatgcggtt aaaatttatc aggcgcgatc
      241 gcggcagttt ttcgggtggt ttgttgccat ttttacctgt ctgctgccgt gatcgcaccg
      301 aacgcgtttt agcggtgtgc gtaacggatg ttatggtaaa tacaatatgc agccagtctt
      361 agagacgaaa agtccgccca gaagactgac ttttcagatc aaccttcagc atctaacttg
      421 tctatcgctt ttttattgcg ccgataggta gccattagtt taccatcgcc tgattgtaag
      481 tcacgacctg cttgctcagc ataggtatcg tttacagtct tagcgccttt tttataggcc
      541 tttttaacat catcaaatcc ggcttggatg acttctttcg gcttttcttg aggtaattta
      601 gccatgctgt gaatcctcta taaggttgat tatttttcca acagataaga cggcatcttc
      661 tgtgtcgctg attttatgat ttatccaacc gtcctctatc tttaatttgt tcactactcc
      721 taatgtaaaa acaatctttt tttcaggaaa gaaaattgca aaggttctac ttttctcttt
      781 ccaatcagag ggcggcgtcg aacggaacgc tacattcctc gatttaagta tcgctttgat
      841 tttactaatt aattcttttt tttgtttatt gctgagattt ttgacgaacc ctttgctgag
      901 attgtctgtg taatcatcaa aagaaatatc aactggtttg tttttttgtt ttttatcaga
      961 tagatgaaca atgtttgaca ctggatatcc tgctttttga tgaatgaata ctcagaagag
     1021 tagcggaact gtcttgttag tcacagaggt agagtatcaa ttctacgata gatgggttcg
     1081 aagatccggc tgagaccgtt cagtacgcgg tcgagatgtt atgttcaatg taatgaccgt
     1141 cgaaattgcc tttacgggca tgaagcaacc aggagcaatc attggcaaag gtaactagag
     1201 ttcaatgcat ggtaatcatt tttgagcttg tgatgcatag agttggttgc tgttccattg
     1261 cgtctcaaac tccgtcaaga tgctcgccgt aaaagcgagc atgtctagta tgcctgttcc
     1321 gctactgatt tttgatgggc gaattcaggg ctggcgagcc accaagaaaa attactttat
     1381 ttcatcaaac aggtgagatg cagtataata aacgctttga tcaaagcgtc tctctgggct
     1441 taatttattt tgcccgccat ttaaaattat ttcttttaat actgatggtg ggaggatggg
     1501 ttttgttctt cttgggtgat ccatataagc ttttgttatt tttccgttta gcgagaactc
     1561 tttatcactt tgatatccaa agttcatctc agtataaatt ctattgatgt ttgtcttgtt
     1621 tttgctcgca atttcataca ccattctggc gagtctggct gatgatctca ttagtctgct
     1681 ggcagatgaa ttgttatatg aggatctatc aataaaggtt cgacacaaag caaaatcact
     1741 ccagatgaat atgtcaaagg cattttgatc taatattggg ctcttgcctt ttgttttcca
     1801 taatggttgc attaaaaaag gtttctggta atcaagaaaa ttttcttgga aagtgtttag
     1861 gcaatttaat atattttctt tctttgaaag aacttcagtt tcattccccc agtgctgtat
     1921 gctttcgcat gtggattcaa atatttttct tatttcatgg aatttatcgc tgcaagagtg
     1981 cattatgcca agggcacaat aagaagtgct tgcaggtctt attacaatct ctgaactcca
     2041 ttcgctctcg ttttctttat atgtcgtgct atctggaagt acggttaatt ttatttctag
     2101 tgcttttaaa aaactattgt ttccttttat aactagatct attcctttta tgtcatcata
     2161 agcatactgt tggtatggtt catatttttt ctcaaattga aattcaagat cactattttt
     2221 taatgttgtg ttaaaaacga agtcaaaaga tacttctttg gcatgcactt ttaaatcctt
     2281 atctaaataa agataaatcg gattcatttt gttgtctctc atataacatg ctagagaggt
     2341 gggaaaagaa ctgttaaatt ggttctttcc ccacagatcg ctctctttcc tgttggtgtt
     2401 ttttaaacca taaagagatt ctattttgct cactttttgc tcctgacgtc aatttattga
     2461 cgtctatggt gtgtttctat atcatgatgt ggagtgatat gataatgagt tttttatgtt
     2521 atgagaataa ttgatctttt tagtgggtgt ggggggttgt ccctaggctt tttgaagggg
     2581 gggtttgatg ttgtaggtgc ttatgatttc tgggatcctg ctattgaatg ttacagagat
     2641 aattttagcc atccgataaa aaaactagat ttgagcaatg ttgatgatgt ggtgagggag
     2701 cttaaagata tagacttcga tatgattatt ggagggcctc cttgccaaga cttcagtcat
     2761 gcaggtttaa gaattgaagg tgctagagct aacttaactc gctcattttc agaaattata
     2821 aaaagaataa agccaaagtg gtttgtgatg gaaaatgtag atcgggcatt aagaagtggt
     2881 gcctatttag aagctcgagg aatctttaaa gaatcagggt atgggttaac agaaattgtt
     2941 ttggatgcca gtaagtgtgg tgttccgcaa aaaagaaaaa gattatttgt aattggaaag
     3001 cttgacgtga gggatggttt tatccttaac gaagtgatgt gcgggatatc gaaagatagt
     3061 atgacagtta gaaattatct tggtgattca ttggggattg agtattatta cagacatccg
     3121 agaaattata acagaagggc tattttctct attgatgaac ctgcgccaac agtaagaggg
     3181 gttaatagac cgatacctga tggatattta gggcatgcag gcgatcctgt cagtatttct
     3241 gagaatgtaa ggccattaac aacctttgaa agagcaagat tgcaaacatt tcctgaagac
     3301 tttaaattta aaggtgcaaa aacaaatttg gagcaaatga taggtaatgc tgttccggtt
     3361 gagttagcaa aatatgttgc ggtgacaatc atggagtatg aaaaaaaaca agtaaaaggt
     3421 atttatgata aagaaggctt tagggcgtgg ttgctgaatg aaaagaaact aacaaaaaga
     3481 acctctagcg atattatatc tagatgttgc agaggtgttt cattttttga ttcagagggg
     3541 gtggattttt ataactgtga aatagatgaa attattatga aactggaaag gcttgaatcg
     3601 tttgtcaggc ttggggttag cctgaagtct cagttaagaa gggcttttaa attatattac
     3661 gaatattgtc gaaggtaata aatacgaatt tatgtctctc aggggagggg gaggggataa
     3721 aaagatcaaa ggatcctctt gtgatccttt ttttctgcgc gtaatctgct gcctgtaaac
     3781 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
     3841 cttccgaagg taactggctt ggtggagcgc agataccaaa tactgtcctt ctagtgtagc
     3901 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
     3961 gtggctgctg ccagtggcgt taagtcgtgt ctttccgggt tggactcaag acgatagtta
     4021 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4081 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
     4141 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4201 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4261 ctctgacttg agcgtcgatt tctgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4321 gccagcggcg cggccttttg ttgtcctttg cctttttgct cacatgttct ttccggcctt
     4381 atcccctgat tctgtggata accgtattac cgcctttgag tgagccgact ccgctcgccg
     4441 cagccgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     4501 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgct cttttcttat
     4561 aatccgcact gatgccacat agttaagcca gtatacactc cgctatcgct acgtgactgg
     4621 ttcagggctt cgccccgaaa ccccctgacg cgccctgacg ggcttgtctg ctcccggcat
     4681 ccgctcacag gcaagctgtg accgtctccg ggagctgcat gtgtcagagg tcttcaccgt
     4741 catcaccgaa acgcgcgagg cagctgcggt aaagctcatc ggcgtggtcg tgaagagatt
     4801 cacaaatatc ggcctgttca tctgcgtcca gttcgttgag tttctccagc agcgttaatg
     4861 tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg
     4921 cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgcgagagga
     4981 tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgc tgtgagggta
     5041 aacaactggc ggtatggatg cggcgggtct gcctgggtga gccggttgcc cgttccggga
     5101 agctgccgac actggcaccg ccgttactgc gtcagctggc cgccatcggg aataacctga
     5161 atcagacagc gaggaaggta aacagcgggc agtggtcttc cggtgaccgg gttcaggtgg
     5221 tggccgcact gatggccatc gagggagagt tgcgcagctt acggcaggtc gtcagggagc
     5281 agggagcgcg ggatgattgt taaatttcat gccaggggaa aaggcggtgg cagtggtccg
     5341 gttgattacc tgctgggaag ggagcgtaac cgggaagggg caacggtgct tcagggtaat
     5401 ccggaagaag tcagggaact catcgatgcc acgccatttg cgaagaaata cacgtccggt
     5461 gttctgtcgt tcgcggagaa ggaactgccg ccgggaggac gtgaaaaagt gatggagagc
     5521 tttgagcgcg tactgatgcc cggtctcgaa aaaaatcagt acagcattct gtgggtggag
     5581 caccaggaca aggggcgtct tgagctgaat tttgtcattc cgaacatgga gctacagacc
     5641 ggaaaacgcc tccagccgta ctacgaccga gcagacaggc cgagaattga tgcctggcag
     5701 acactggtga atcaccatta cgggctgcat gaccccaacg ccccggagaa ccgccggata
     5761 ctgactctct ctgataacct gccggaaacg aaacaggcgc ttgctgagag cgtcacacgc
     5821 gggatagacg ctctttatca tgtaggggag ataaaaggcc gtcaggatgt gattcaggcg
     5881 ctcacagagg cggggcttga agtggtcagg gtgacgcgaa gcagcatcag cattgcagac
     5941 ccgaacggcg ggaagaatat caggctgaaa ggagcgtttt atgagcaatc ttttacagac
     6001 gggcgcggag ttcgagaaaa agctgaaaga gagagccgaa tctacagaga aaatgctgaa
     6061 cgacgagttc aggaagctcg gcgagtctgt cagcgaggct gtgacctcaa acgagacgaa
     6121 aatcagagac gctatagccc tgttcacagc ctcgacagag aaatcgctgg aaaaacacag
     6181 ggaaggggtg aaagaggcga tgatgcagca caggaaggac gtgttaaagc tggcagggaa
     6241 tacgggcatg atgttactgg ggatagtctt tctcctgttt accgcgagtg gcgggacgct
     6301 ctggtatctt ggcgggagga tacaggcgaa cctggaagaa atcaggaagc aggaagagac
     6361 attgcagaaa ctgaacgcga agacatgggg cgtggagttt gtgcaggacg ggagaaggaa
     6421 atttctggtc attccgcaag ggatgtcagc agaggtgacg cactatcagg ggaaggactg
     6481 gatacaactg acggagtgat acggaatgac agagctggaa acacatttgc tgaacgcctt
     6541 agagcagctg caacaggact atatgcagcg gctgaacgaa tgggagagcg ccttcgtgga
     6601 attgcagaag atgttttcgc ttacgcaacg ggacaacgcg atgctgaacg agcgggtcat
     6661 gcagttgagt cagcaggtgc agcacttgag cgagcggaca gaacgcttga gccag
//
