LOCUS       NZ_AP023457             9294 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain M941 plasmid pM941-c, complete
            sequence.
ACCESSION   NZ_AP023457
VERSION     NZ_AP023457.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00237915
            Sequence Read Archive: DRR238844, DRR238850
            Assembly: GCF_014905815.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sugawara,Y., Akeda,Y., Hagiya,H., Zin,K.N., Aye,M.M., Takeuchi,D.,
            Matsumoto,Y., Motooka,D., Nishi,I., Tomono,K. and Hamada,S.
  TITLE     Occurrence and characterisation of Klebsiella pneumoniae sequence
            type 11 international high-risk clone harbouring blaNDM-5 in a
            clinical setting in Myanmar
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Sugawara,Y., Akeda,Y. and Hamada,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-SEP-2020) Contact:Yo Sugawara Osaka University,
            Research Institute for Microbial Diseases; 3-1 Yamadaoka, Suita,
            Osaka 565-0871, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.5
            Genome Coverage                   :: 327x
            Sequencing Technology             :: GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 10:03:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,569
            Genes (coding)                    :: 5,362
            CDSs (with protein)               :: 5,362
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 125 of 207
            Pseudo Genes (incomplete)         :: 82 of 207
            Pseudo Genes (internal stop)      :: 15 of 207
            Pseudo Genes (multiple problems)  :: 15 of 207
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023457.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="M941"
                     /isolation_source="human sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pM941-c"
                     /geo_loc_name="Myanmar:Yangon"
                     /lat_lon="16.7788 N 96.1490 E"
                     /collection_date="2018-12"
     gene            complement(1481..1630)
                     /locus_tag="JLF44_RS28245"
     CDS             complement(1481..1630)
                     /locus_tag="JLF44_RS28245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(1715..1972)
                     /locus_tag="JLF44_RS28250"
     CDS             complement(1715..1972)
                     /locus_tag="JLF44_RS28250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(1982..3667)
                     /locus_tag="JLF44_RS28255"
     CDS             complement(1982..3667)
                     /locus_tag="JLF44_RS28255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            4070..4489
                     /locus_tag="JLF44_RS28260"
     CDS             4070..4489
                     /locus_tag="JLF44_RS28260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            4795..5058
                     /locus_tag="JLF44_RS28265"
     CDS             4795..5058
                     /locus_tag="JLF44_RS28265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            5048..5347
                     /locus_tag="JLF44_RS28270"
     CDS             5048..5347
                     /locus_tag="JLF44_RS28270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5409..5786)
                     /locus_tag="JLF44_RS28275"
     CDS             complement(5409..5786)
                     /locus_tag="JLF44_RS28275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            5855..6034
                     /locus_tag="JLF44_RS28280"
     CDS             5855..6034
                     /locus_tag="JLF44_RS28280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6062..6592)
                     /locus_tag="JLF44_RS28285"
     CDS             complement(6062..6592)
                     /locus_tag="JLF44_RS28285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6599..7330)
                     /locus_tag="JLF44_RS28290"
     CDS             complement(6599..7330)
                     /locus_tag="JLF44_RS28290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(7330..9294)
                     /locus_tag="JLF44_RS28295"
     CDS             complement(7330..9294)
                     /locus_tag="JLF44_RS28295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..8667
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          800..1378
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6603..7330)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 gttctctctc cttcagacga tggtttgccg gcgtgatgtg cagcccccgc tgcacaagcc
       61 gcggacatgc cccaaggcat ggcggcggcg gtcacgtccg tggtgattgc gttcagcttt
      121 caccacggag gccagcctct ttccgcccca tgcggaaaag gcgcagcccc ggaggaacaa
      181 cgagcacgcc ccagcgtgtg agaggaaaag gcggcacccc agtgcggtct tttccggtgt
      241 tccggccccc gtcagagttc gccatagcga gcggctgacc acccgcaaaa tcaggagcag
      301 ctttgctgct tcgccaggtg cgtccagcac cgtggatttt tgtgggtggt caggcgtgag
      361 cctgacgggg gccgggtgaa tttctggcgt ggccggcgtc cagccggacg gagccagaaa
      421 gagggggaga gaccgtgcgt cggtggcgtc cagccagcgt aagcagggga tatccccctg
      481 aggtcagcca cgccctcgcg gggatgacct gtatttctcc gtaaggagaa aaaagcaaca
      541 gagataggtt aacgtaaaat gttcagcgaa aaaccgatat gattcaccta tcctggtaat
      601 ttattatcag ggaatataaa gacttaatca ggggacaata tgccacatca gcgcgtccgg
      661 tgtccgtctt gtaggtgccg gggaaattgc gcctcctcgc tcactcggtc gctacgctcc
      721 ggccgtgaga ctgcggcggg cgctgcggac acatgcaaag ttacccacag attccgtgaa
      781 taagcagggg actaacatgt gaggcaaaac ggcaaggcca cgcaggtggc gtttttccat
      841 aggctccgcc ctcctgactg agttcacata aacagacgct ttttcggtgc atctgtggga
      901 gccggtggct caaccatgaa tcagtcagga gtggcgaaac ccgacaggac ttaaagatca
      961 tcaccgtttc cgatgaatcg ctccctcttg cgctctcctg ttccaaccct gccgtttccc
     1021 ggatacctgt tccgcctttc tcccttgcgg gaagtgtggc gctttctcat agctcacact
     1081 ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg ggctgtaagc aagaaccccc
     1141 cgttcagccc gactgctgcg ccttatccgg taactgttca cttgagtcca acccggaaaa
     1201 gcacgcaaaa cgccactggc agcagccatt ggtaactggg agttcgcaga ggatttgttt
     1261 agctaaacac gcggttgctc ttgaagtgtg cgccaaagtc cggctacact ggaaggacag
     1321 atttggttgc tgtgctctgc gaaagccagt taccacggtt aagcagttcc ccaactgact
     1381 taaccttcga tcaaaccacc tccccaggtg gttttttcgt ttacagggca aaagattacg
     1441 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctactgaacc gcgatccccg
     1501 tcagttcaga ggaggacgat ggtgccaccg ttccaccctg aacatcccgg atatagtttg
     1561 cctgacatgc gaccagaaga aagcctgaaa aaatcaatat aaaaagaaaa acaaccttta
     1621 cctttttcat ataccttccc tgtatttaaa aaccccggta ttcatccctg aacaccggaa
     1681 gtctgtattt caaatcatcc ttgataactt ttgcttacca gttaccatcc cggtaaacaa
     1741 aggatacaaa gtaatcaaat ttactaatat cgattacatt tttaaagtgt ggctgtaata
     1801 tcgaaaccca tgatttttca acatcaaacc aaccattatt aatattatcc tttaaaggca
     1861 tccccagact ttcaatgaca gaaccatcat caccgaagtc ttttgagtat tcaccacctt
     1921 taaactcttc ggttttctta tcaaaccaat gaatatgtaa tttaagcccc atatttacct
     1981 cttaaagata ttttttaatg tttcgttttg gatccggccc tttaacctgc ttacccgttt
     2041 ttggatcgaa tgccccgagg tgttcgccat cactggcgcg gtacccttca agctcaccgt
     2101 gctgggaatc ccattcataa attttacgct ttttatcacc ataccatcgg gcccgcttac
     2161 caccaccacc ttgtttaggg gttttaggtt taccctcttt caactctccc aaccccttaa
     2221 tatcttcagt cttgggatca ggaaaataat catggccgta atctttagtt cccttacgag
     2281 gttttttctt ttcctcattt aatttatttt ctgcatcctt cgcttttttc tccttttgtt
     2341 tccggctctc catcgccccg gaaagcgcag ccttatccct ggataaggat tcattcattt
     2401 cgtttaaaga tgtctgggca gcttttaatt tctcattctg attctgaata tccttatcaa
     2461 gatagcgaac ctgatcctgc cacccggagc catattcatg aggcgggtta taagtcacaa
     2521 aatcatcttt atttgcctct gcctctttta cctttttatc ggcttcctga acagcagccc
     2581 ttgcaccggg gatcgccttc tctgtcgatg caatgcgact gtttaaggtc gcaatatttt
     2641 tatcttcagc atccagctca gctttcgctt tatcatactc tcttttcagc ccctcttccg
     2701 ggtgagcagc gtcccatgcc tgctggcgac gcttttcttc ctccagacgc tgttttacct
     2761 gtgccggagt aagaacatcc gtcaccgaca catagaccgg tttctgtccg ctctctttcg
     2821 ggaaacgaat aacggcttca cgggagttac caccggccgt aaaaccagcc ggacgtgaat
     2881 cacctttttc agcaataatg ccttttggcg gggctttcgc ggccggaaca cctttaggta
     2941 cgctcacctg cagagacggg agacccggaa tcgacacact gaataccccc ggacgttttg
     3001 tcggtttcgc atccaccaca ggaacactca ttggccggcc ggtgacaacc gcaatatgct
     3061 gccgctcgtc cttcacaata tccgccacac gtttagtgac gctgaccgtc gcctggtcca
     3121 gcggcaggga gctgaccggc gtctccgtta ccgtgtctgc cggcaatgac gtcacgattt
     3181 ttgacatcat tttcggatca tcttttgcta tctcagaagg cagcactccg taaatcgcaa
     3241 tcccccacag accaaactta aacggacctt tcagggcagc cagcacatca gcaacagcgg
     3301 ccgacaacgc atcgccggaa acggataaag ccagtccttc agcccccgga gtggccaagg
     3361 caggcagacc gaaggccatc gcggtggccg aagactgtcc gccaccagag ctcccgctat
     3421 gattgccgct gttaccgcca ttaccaccgg gtttactccc gccgttacca aactcccccg
     3481 ggttatagtt atgaatatcc ccgttaggtg tatgcgtcgt accccagccc ggcccgctgt
     3541 ttggatccgt accaccacct gatgaagggc cacctttacc tgatgtaccg ttcacattcc
     3601 cactggcctg accaccattg tgtcccagac ctgaattacc cggacctcgt ccgtctccgc
     3661 cactcatata cttttcctct ttgactttta aaacaataaa ctaaaataaa atactgtaca
     3721 tataaccact ggttttatat acagtataaa aattacgctg ccttgttttt catgtcaagt
     3781 ctgtaaattt gcgtttttgt gaaaatgatc aaaaaaacag ccactcacac catgcgcaat
     3841 cccctccaga tgcaacaaaa accagcacaa acggcgccgt tctccggcct cagtatgttt
     3901 tactgaaatc attatatgag ataccagaaa gccccctccg ggacaggaaa agaaacgggg
     3961 ggtctggttc gggttgttcg gtcactgaac acggtcagaa actgatgata agatagcctg
     4021 cggatatata actcaatgag aaaaaagaat taaaggtaag gagacgctca tgagacgttc
     4081 cggcagcaaa ctgtgtctta tcgccatgat aacagcgaca gcaactctca ctgcctgtgc
     4141 tccaaagggc agcgtggaac agcatacccg gcattacgta tatgcttcag atgacggttt
     4201 tgatcccaat ttttccaccc agaaagctga cacaacacga atgatggtgc cttttttccg
     4261 gcagttctgg gatatgggcg caaaggataa agcgaccgga aaatcacgga gtgatgtgca
     4321 gcaacgcatt cagcagtttc acagtcagga atttttaaac tcactccaag gcacaacaca
     4381 atttgcgggt accgattacc gcagcaaaga cctcacaccg aaaaaatcaa ggctactggc
     4441 tgacaccatt tcagctgttt atctcgatgg ctatgaaggc agacagtaag tggatttacc
     4501 ataacgcgtc attacgcgca ccgctcagac gggctcaggg cgtttccggc ggctacggga
     4561 taaaacaggc aacaaccccc ccggaaaact gccggaaacg cgcccgataa attttatcct
     4621 aatgcatgcc tatgcacgca actgcatggc atatgttttg acgtaaaatg gatgccgtaa
     4681 gccctttttt accgactttc tgcttcatca gcctgactga cacgaccata ttctttcaga
     4741 ccgccccctg ctaaacttcc ttcatattgc cgcattaacc cgggaggctc cgcaatgagc
     4801 aacactacac actacgaaaa cgccaacttt cttcgggaac tggctgaaag cctgccccgc
     4861 atactgccgg aaggtggccc ggacaaagct gcgctgttgc agcgactggc taacgaagaa
     4921 ctggcccagg cagagtatga agatcaggtt cgcgccaaag tgaccgccgc ccgtgctgac
     4981 acgcgtccgg gtatgaccac ggaacaactc cgccagcgcc tgcatggccg ttatcaggaa
     5041 ctgcgcgatg ccgtatgacg tggaatggaa gcagggagcc atcgaggatg taacgacgct
     5101 gtttgactat atcgcggaaa attcatccct gtgggatgcg cagaacgtta ccgaccgggt
     5161 tctcgctgcg gcggacaaac tggccgactt cccgcgactc tatgaagtgg actcacgcta
     5221 tggcgaggac gttcgccgga tcagtctgat gggacagcat gtcctctatg atgttgatga
     5281 ccagaccaga aaagtccggg ttctggccgt ggtcggccag cgccagaatc cccatattgt
     5341 tctctaataa ccggtgacgg ggacgcaatt cattgcgtgg ccgtcgccgg ttggacaagc
     5401 acagcgcgtc agcgttttcc ccacggccac caccggcgcg gtgttccggc cggggtctct
     5461 gctgtctccg gtttgtgttc cagcagtctc atggactgca tttcctgccg tagcccggcc
     5521 acctcctccc tgagtgcttt tatctccgcg atcaacgcgt cggaggccat tttttcagtc
     5581 tcacccgaca gtctcaactc gtcccgctcg tgagtctcat cctgtcttaa ctcaccgtac
     5641 acccggataa gctcgctggt atccaccacc cggccgccat cagcttccgt acggtatgat
     5701 acgcggcctt tcgccatatc ccgatacagg gaacttcggg ctttacccgt cagttccgtc
     5761 gcctgtctca ccgtcaccca cgccattgtc tcaccccgct atttttgaga cagtatacct
     5821 gacaggcgca caccatgccc ggaaggaaag aatcatgaca aaacaggaaa aaacagccct
     5881 caatatggct aaatttatta aggagcagtc gctgctattg ctcgataaac tgaatgagct
     5941 ggatatggat accccggccg acatgtgcga gcggctacac gaggatgcgg aaacactctg
     6001 gcaggtgatg cgggataagc tcggggagga ataacagcac cggcatggcc ggtgctggca
     6061 tttatttttt gccgaacggc caccaccggc gggcaggttt gtcctccggc agttcaggta
     6121 ccggcacgtt aaaatagcgg ctgttatatt cccgcatcag ctccgctttc agcttctcgt
     6181 tcaccgcgct ttccccgtgc cgctcactca ccacgtcaat catcgcgtta atctgctccg
     6241 gtgagcactt cccggcatca accacctgca caccgttctc gatggcgaac cgctccacct
     6301 tgcgatagag gtaatcacac agcttcattc ctgtgggttt gttccgttcc cattgcggat
     6361 ttttaccagt gaaaaagtac gtggcttttt cgtcgctgtt gagccgctcg tcatacatcc
     6421 cgatggattt catcatcgcc agatgctggg cccggtcgac aaaacagaaa acatacgccc
     6481 cggtctgcca ggcgagcacc accatatctt ccagatgctc gctggtcggc atgatgcagc
     6541 gctcgccgtt ccggtcctcc gcgtcttcaa taaataccat gtacagcgcc ataatccctc
     6601 actccgttgt cataaattta aagcacgcaa acaaccgttc acgatccagc tctgccgttt
     6661 tgcgggcctc gccggtctgt gggttcacga tttcatccac cgacaggatt gcccgctgca
     6721 ccctgacctg cgtcgcccgg tccgggcaga tgtaccagat ttcatcgatg ccgttagctt
     6781 tgcggttgaa cagatacccg gacaccactt cctgataccg gcgacggctc ttgacggtgc
     6841 gctccacctc aaacgccacc gttttaccgt ccagctccgc cagggcgtcc ggcactttca
     6901 tgcctttaag actcagacgg tgcagaatgc gccagccggt ggcccctctg gcttccatat
     6961 caaggcgcag ggattgcacg gccagccggt gcggcagatg tgctgccgac acccttgacg
     7021 gctgaaacgg aataacatcc gccagcggtt cgtcctcgtc gaacgagaac gccacgccat
     7081 gcggcgtcag cccccacacg ctgacctgac ggccgtccac cggatatttg gtccggacca
     7141 gcagcccttt cgccaccagg ctgtcgagga agcggtagac cggctgcacc gccgcaatcc
     7201 ccagccgccg cgcaaccagc tccgccgtgg aacacagctc ttcccgcaac cagcgcaggg
     7261 tcagccgggt tttctccgcg ttccgcgcct gtcgtttcgc gtggctgacg ttgtacagct
     7321 ccagcgccat cagtaaatcc ccgcaaaatc atcatccgct ttcggcccgc cgccgtcggc
     7381 cgggggccgg aactccgcca ggggatcggt tttcgccagc accgggaaac gctgcaacgg
     7441 gatttcccgg cgctcaaccc gcagcggact gctgaacgcc atccgggcca cacccagccc
     7501 ggtcatgacc gcgaacccgt ccggcaggtg ttgcagggtg ttcacgtcaa acagcggccg
     7561 cgcctccttc tggatgaagc tgtccccgct gatatgctcc acgttccccg cctcccggct
     7621 ggcacggcgg cgctccacgt ccaccctgat ttccccggtc tgccccgccg cccactgcgc
     7681 ggactccagg ctggccgccc ggtaaaacca gcggatcggc gtgttatcca gtaccgtggt
     7741 tttcacaaat tcagcgggca gatcctgccc gcactgcccg aaatcgccca gcgactggtg
     7801 cgcgagcaac agattgcagt cccggctgcg cagcgtcccc agtgcgttca gtacatactt
     7861 cgacagcaga tatttaattt catcgagcat cacgctgacg tgtggccact cccggaactc
     7921 gtcccgcgca atgatgattt gcgcgcagcg ggcaaacagc attttttgca cacggatcac
     7981 cgcctcgtcg tccatcgagc cgataacgta caggcagccg ccgccgttga taatccccgc
     8041 cacatcgatg ccactgtccg tctgcaggac gctcaggtca gcgacgtttt ccagcctagt
     8101 gatcaacccc ttcacgtcct ttttcagggc ctccggcagc gcgtacgccg ccgccagcac
     8161 ctgctgaatg tttgccccct gcggaaactg ctccgcaatc agcttcgccg ctttctgctc
     8221 gctgatgcgg tagtggtccg ccacgtcgcc ggtctcgccc aggttaagcc cggcaatcag
     8281 caggtttttc agcgcgtact ggtcgagatc gcggaacaga ttcagctgcg ccggtttccc
     8341 cggccgcaga tccagcaggg tgaagtccgg acagtgcgcc cggaagacgt gcggcgccca
     8401 tgcgtcccct ttggggtcga agtacaccac cgcgtccccg aaatcgcgta cgcagcgaat
     8461 gagcgcgttg gtggccatca cgctcttgcc gctcccgggc aggccgagga tctgaatgtt
     8521 ggtcttgcgc caggtatcac gcggaaccag caccggcttg ccctgctcat ccttacccag
     8581 aaacaccgcc tgctcctgcc gggcctgctc cgaggaaaaa tcggccagcg cattaaccgg
     8641 taccggctcc ccgaaatgca ggtggcgcac gtcggtcatc tctcctgtcc gctggctgct
     8701 gcgccggctc tcctgattca gtttcgcgtt caccgcctcg tcagcagccg caaaatgacc
     8761 gtcaagcagc aggaacgcca gcagccgcgc caacacaatc ccgacagcca gcccgcccgc
     8821 gcaccaggca atcccgaaca ggttagccgg ctgcagcagg taataatctg cgtttgcgta
     8881 gaggtccgga cgcaggacgg tttctcccca gctctcgata aacacggcga caaccagccc
     8941 ccccagaagg gccagaccgg ccgcgccggt cagccagtac gcgtagttcc agacatgtcg
     9001 acgtgccctg accatccgcc cgggcgaatc cagcgaggcc agcaccgaaa atggcgctgg
     9061 ccgtttatta tcgcgcttat agcgatcggc atactcccgt cgctccttat gactcagccc
     9121 cacaaaacgc atccgtccgg cccaaagttc agccagaccg aaaaacaggc cgataactag
     9181 gaaagccccg gccagaccgt aatactcctg gtacagccag ctgacccacg gataaatctg
     9241 ccgggccagc tccggaatgt gccacttcca ggcaagcgaa tccgtattaa acat
//
