LOCUS       NZ_AP023451            22328 bp    DNA     circular CON 17-JUN-2024
DEFINITION  Enterobacter roggenkampii strain OIPH-N260 plasmid pN260-4, complete
            sequence.
ACCESSION   NZ_AP023451
VERSION     NZ_AP023451.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00235761
            Assembly: GCF_016756655.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Umeda,K., Nakamura,H., Fukuda,A., Matsumoto,Y., Motooka,D.,
            Nakamura,S., Yasui,Y., Yoshida,H. and Kawahara,R.
  TITLE     Genomic characterization of clinical Enterobacter roggenkampii
            co-harbouring blaIMP-1- and blaGES-5-encoding IncP6 and
            mcr-9-encoding IncHI2 plasmids isolated in Japan
  JOURNAL   J Glob Antimicrob Resist 24, 220-227 (2021)
   PUBMED   33385587
  REMARK    DOI:10.1016/j.jgar.2020.11.028
REFERENCE   2  (bases 1 to 22328)
  AUTHORS   Umeda,K., Nakamura,H., Fukuda,A., Matsumoto,Y., Motooka,D.,
            Nakamura,S., Yasui,Y. and Kawahara,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2020) Contact:Kaoru Umeda Osaka Institute of
            Public Health, Microbiology; Tojo-cho 8-34, Tennoji-ku, Osaka, Osaka
            543-0026, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.6
            Genome Coverage                   :: 1117X
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore
            Technologies MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016756655.1-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 02:47:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,413
            CDSs (total)                      :: 5,297
            Genes (coding)                    :: 5,045
            CDSs (with protein)               :: 5,045
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 252
            CDSs (without protein)            :: 252
            Pseudo Genes (ambiguous residues) :: 0 of 252
            Pseudo Genes (frameshifted)       :: 108 of 252
            Pseudo Genes (incomplete)         :: 134 of 252
            Pseudo Genes (internal stop)      :: 37 of 252
            Pseudo Genes (multiple problems)  :: 23 of 252
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023451.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..22328
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="OIPH-N260"
                     /isolation_source="human bile"
                     /host="Homo sapiens"
                     /db_xref="taxon:1812935"
                     /plasmid="pN260-4"
                     /geo_loc_name="Japan:Osaka"
                     /collection_date="2019"
     gene            join(22218..22328,1..645)
                     /gene="repE"
                     /locus_tag="OIPHN260_RS26600"
                     /old_locus_tag="OIPHN260_53520"
     CDS             join(22218..22328,1..645)
                     /gene="repE"
                     /locus_tag="OIPHN260_RS26600"
                     /old_locus_tag="OIPHN260_53520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="WP_000807690.1"
                     /translation="MAEIAVINHKKRKNSPRIVQSNELTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGSLQEHDGICEIHVAKYAETFGLTSAEASKDIRQALKGFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGVVSLKIDWIMERYQLPQSYQRMPDFRRRFLQASVDEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTIE"
                     /name="repE"
     gene            982..1687
                     /locus_tag="OIPHN260_RS26605"
                     /old_locus_tag="OIPHN260_53540"
                     /pseudo=""
     CDS             982..1687
                     /locus_tag="OIPHN260_RS26605"
                     /old_locus_tag="OIPHN260_53540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /name="IS6-like element IS26 family transposase"
     gene            1825..2109
                     /locus_tag="OIPHN260_RS26610"
     CDS             1825..2109
                     /locus_tag="OIPHN260_RS26610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_159186868.1"
                     /translation="MSRLSHLSEHSAAAVCKGPSSLNARCLPGGEDDVSSSLSPHRERS
                     LLLLAHIGGSPVTLSSVAKSADVIPCVNEKVLMVNPAANEESVVRLAAR"
                     /name="hypothetical protein"
     gene            2392..3807
                     /locus_tag="OIPHN260_RS26615"
                     /old_locus_tag="OIPHN260_53550"
     CDS             2392..3807
                     /locus_tag="OIPHN260_RS26615"
                     /old_locus_tag="OIPHN260_53550"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725197.1"
                     /GO_function="GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="WP_004180506.1"
                     /translation="MLLPVIMAGGTGSRLWPMSRELYPKQFLRLFGQNSMLQETITRLS
                     GLEIHEPMVICNEEHRFLVAEQLRQLNKLSNNIILEPVGRNTAPAIALAALQATRHGDD
                     PLMLVLAADHIINNQPVFHDAIRVAEQYADEGHLVTFGIVPNAPETGYGYIQRGVALTD
                     SAHTPYQVARFVEKPDRERAEAYLASGEYYWNSGMFMFRAKKYLSELAKFRPDILEACQ
                     AAVNAADNGSDFISIPHDIFCECPDESVDYAVMEKTADAVVVGLDADWSDVGSWSALWE
                     VSPKDEQGNVLSGDAWVHNSENCYINSDEKLVAAIGVENLVIVSTKDAVLVMNRERSQD
                     VKKAVEFLKQNQRSEYKRHREIYRPWGRCDVVVQTPRFNVNRITVKPGGAFSMQMHHHR
                     AEHWVILAGTGQVTVNGKQFLLTENQSTFIPIGAEHSLENPGRIPLEVLEIQSGSYLGE
                     DDIIRIKDQYGRC"
                     /name="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
     gene            3829..5211
                     /gene="rfbK"
                     /locus_tag="OIPHN260_RS26620"
                     /old_locus_tag="OIPHN260_53560"
     CDS             3829..5211
                     /gene="rfbK"
                     /locus_tag="OIPHN260_RS26620"
                     /old_locus_tag="OIPHN260_53560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_166649022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O9 family phosphomannomutase RfbK"
                     /protein_id="WP_040079938.1"
                     /translation="MTQLTCFKAYDIRGELGEELNEDIAYRIGRAYGEFLKPGKIVVGG
                     DVRLTSESLKLALARGLMDAGTDVLDIGLSGTEEIYFATFHLGVDGGIEVTASHNPMNY
                     NGMKLVRENAKPISGDTGLRDIQRLAEENQFPPVDPARRGTLRQISVLKEYVDHLMGYV
                     DLANFTRPLKLVVNSGNGAAGHVIDEVEKRFAAAGAPVTFIKVHHQPDGHFPNGIPNPL
                     LPECRQDTADAVRAHQADMGIAFDGDFDRCFLFDDEASFIEGYYIVGLLAEAFLQKQPG
                     AKIIHDPRLTWNTVDIVTRSGGQPVMSKTGHAFIKERMRQEDAIYGGEMSAHHYFRDFA
                     YCDSGMIPWLLVAELLCLKNSSLKSLVADRQVAFPASGEINRKLGNAAEAIARIRAQYE
                     PAAAHIDTTDGISIEYPEWRFNLRTSNTEPVVRLNVESRADTALMNEKTAELLNLLKEE
                     SL"
                     /name="rfbK"
     gene            5217..6002
                     /gene="wzm"
                     /locus_tag="OIPHN260_RS26625"
                     /old_locus_tag="OIPHN260_53570"
     CDS             5217..6002
                     /gene="wzm"
                     /locus_tag="OIPHN260_RS26625"
                     /old_locus_tag="OIPHN260_53570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O9 family O-antigen export ABC transporter
                     permease subunit"
                     /protein_id="WP_040079937.1"
                     /translation="MFSAIYRYRGFIIDSVKRDFQSRYQTSFLGAAWLILQPIAMISVY
                     TLIFSELMRARLAGMDGPFAYSIYLCSGVLTWGLFTETLNSLVNVFLTNANILKKLSFP
                     RICLPIIVTASAFINFLIIFGLFVLFLIVTGNFPGMIFFEIIPVLIVQMLFTLGLGIIL
                     GVLNVFVRDVGQFVNILLQFWFWFTPIVYVSKTLPEWVSGLLAYNPMATIIGSYQNVML
                     YHQSPNWLALFPVTVLSVILFLFAWRLFKKHAADIVDEI"
                     /name="wzm"
     gene            6005..7298
                     /gene="wzt"
                     /locus_tag="OIPHN260_RS26630"
                     /pseudo=""
     CDS             6005..7298
                     /gene="wzt"
                     /locus_tag="OIPHN260_RS26630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="O9 family O-antigen export ABC transporter
                     ATP-binding subunit"
                     /name="wzt"
     gene            7318..9750
                     /locus_tag="OIPHN260_RS27190"
                     /old_locus_tag="OIPHN260_53600"
     CDS             7318..9750
                     /locus_tag="OIPHN260_RS27190"
                     /old_locus_tag="OIPHN260_53600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="WP_244857422.1"
                     /translation="MTKNLKTLVSELPEIYQTIFGHPEWDGDAARDCNQRLDLITEQYD
                     NLSRVLGRQLKVLDLGCAQGFFSLSLASKGATIVGIDFQQENINVCRALAEENPGFAAD
                     FRVGRIEEVIDALEEGEFDLAIGLSVFHHIVHLHGIDEVKRLLSRLADVTQAVILELAV
                     KEEPLYWGVSQPDDPRELIEQCAFYRLIGEFDTHLSQVPRPMYLVSNHRVLLNDFNQPF
                     QHWQNQPYAGAGLAHKRSRRYFFGEDYVCKFFYYDMPHNTLMAEESQRNKNELHNEMCF
                     LAQPPTGFDTPALLAHGENAQSGWLVMEKLPGSLLSDMLVAGEEIDREKIIGTLLRSLA
                     VLEKQGLWHDDVRPWNVMVDARQHARLIDFGSIVTTPQDCSWPANLVQSFFVFVNELFA
                     ENKNWTGFWRSAPVHPFNLPQPWSNWLYAVWQEPVGSWNFALLLALFDKKEKLPSAEQQ
                     RGATEQWIIAQETVLLELQSRVRNESASSEAMRGEIHALEQQIVQLQAEQQRGATEQWI
                     IAQETVLLELQSRVRNESASSEAMRGEIHALEQQIVQLQAEQQRGATEQWIIAQETVLL
                     ELQSRVRNESASSEAMRGEIHALEQQIVQLQAEQDALVEKAVLPVSISHELSWLTENME
                     QLTALLGSAKAQSQAEIEPELPLETAELLQRLETANREIHHLNNENQQLRQEIEKIHRS
                     RSWRMTKGYRYLGLQIHLLRQYGFVQRCKHFIKRVLRFVFSFMRKHPKVKHTAVNGLYK
                     LGLYQSAYRLYRRMNPQPYSQYQADAQILSQTELQVMHPELLPPVVYEIYLKLTKNK"
                     /name="methyltransferase domain-containing protein"
     gene            9761..>10450
                     /gene="wbdA"
                     /locus_tag="OIPHN260_RS26645"
                     /old_locus_tag="OIPHN260_53610"
                     /pseudo=""
     CDS             9761..>10450
                     /gene="wbdA"
                     /locus_tag="OIPHN260_RS26645"
                     /old_locus_tag="OIPHN260_53610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105458066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="polymannosyl
                     GlcNAc-diphospho-ditrans,octacis-undecaprenol
                     2,3-alpha-mannosylpolymerase WbdA"
                     /name="wbdA"
     gene            <10562..12397
                     /gene="wbdA"
                     /locus_tag="OIPHN260_RS26650"
                     /old_locus_tag="OIPHN260_53620"
                     /pseudo=""
     CDS             <10562..12397
                     /gene="wbdA"
                     /locus_tag="OIPHN260_RS26650"
                     /old_locus_tag="OIPHN260_53620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105458066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="polymannosyl
                     GlcNAc-diphospho-ditrans,octacis-undecaprenol
                     2,3-alpha-mannosylpolymerase WbdA"
                     /name="wbdA"
     gene            12444..>12974
                     /locus_tag="OIPHN260_RS26655"
                     /old_locus_tag="OIPHN260_53630"
                     /pseudo=""
     CDS             12444..>12974
                     /locus_tag="OIPHN260_RS26655"
                     /old_locus_tag="OIPHN260_53630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809192.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /name="glycosyltransferase family 1 protein"
     gene            complement(13128..14108)
                     /locus_tag="OIPHN260_RS26660"
                     /old_locus_tag="OIPHN260_53640"
     CDS             complement(13128..14108)
                     /locus_tag="OIPHN260_RS26660"
                     /old_locus_tag="OIPHN260_53640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.2"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="WP_000019402.1"
                     /translation="MSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINIEYMKASIRAKVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH"
                     /name="IS5-like element IS5 family transposase"
     gene            complement(14451..15098)
                     /locus_tag="OIPHN260_RS26665"
                     /old_locus_tag="OIPHN260_53650"
     CDS             complement(14451..15098)
                     /locus_tag="OIPHN260_RS26665"
                     /old_locus_tag="OIPHN260_53650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241263.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_024262784.1"
                     /translation="MTHTVPQSFVRAYLRASTSEQDASRALAAIDSFADERGLNICNYY
                     IENESGSRLDRPELFRLLNDCRQNDILLIEDVDRLSRLTGEDWNSLKKLIRQKDIRVMA
                     INVPTTWIASASHDFDSRMFAAINDMLLDMLAAVARRDYEQRRERQRQGINKAQKEGKY
                     KGRQVNQARYDAINRLLDSGSSWSQVQKIMACSRGTISSAVKSREDKRALSK"
                     /name="recombinase family protein"
     gene            15435..16097
                     /locus_tag="OIPHN260_RS26670"
                     /old_locus_tag="OIPHN260_53660"
     CDS             15435..16097
                     /locus_tag="OIPHN260_RS26670"
                     /old_locus_tag="OIPHN260_53660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_032435805.1"
                     /translation="MGKILLVVSDKGGVGKSTYVANTGAMLVNKGKSVIILKTDKNPDL
                     LSWNEKRIDNGLLTIPVHESYGNVSNEIKRLSKLCEVLIVDCPGHDSQEFRSALTVSDI
                     VVTLVKPSSDFESETLTSVTEKVRTAQKTNSALEPWVLFTRINSAKPRHRKAAIELDKL
                     LRSENIWIQPLKTRISELDVYESACNEGAGVHDVSRASSLSTAKAQIELVAQEIGIL"
                     /name="division plane positioning ATPase MipZ"
     gene            16197..16481
                     /locus_tag="OIPHN260_RS26675"
                     /old_locus_tag="OIPHN260_53670"
     CDS             16197..16481
                     /locus_tag="OIPHN260_RS26675"
                     /old_locus_tag="OIPHN260_53670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032435806.1"
                     /translation="MALKLNRPNVEESQQPATSAETARFISGATRTPLVGKPKLMNFRL
                     PGHFEEILESESQRTGQSKTTVLKAALAAFDNLDENQKNHWLLESAKLG"
                     /name="hypothetical protein"
     gene            16653..>17345
                     /locus_tag="OIPHN260_RS26680"
                     /pseudo=""
     CDS             16653..>17345
                     /locus_tag="OIPHN260_RS26680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            18108..18443
                     /gene="stbA"
                     /locus_tag="OIPHN260_RS26685"
                     /old_locus_tag="OIPHN260_53700"
     CDS             18108..18443
                     /gene="stbA"
                     /locus_tag="OIPHN260_RS26685"
                     /old_locus_tag="OIPHN260_53700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006796897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein StbA"
                     /protein_id="WP_053259092.1"
                     /translation="MNKPADLKPRNLSAAVRLRLNEIENWLDRGLTRHEIAEILDSEYS
                     FSVTAKGLEMALYRTRKNRKNVLYNTQPPEPEAQESEKAESPGIIDKEFFNKIGEDFNP
                     KKFNKKF"
                     /name="stbA"
     gene            18452..>18637
                     /locus_tag="OIPHN260_RS26690"
                     /old_locus_tag="OIPHN260_53710"
                     /pseudo=""
     CDS             18452..>18637
                     /locus_tag="OIPHN260_RS26690"
                     /old_locus_tag="OIPHN260_53710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011279206.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /name="plasmid stabilization protein"
     gene            complement(18654..>18989)
                     /locus_tag="OIPHN260_RS26695"
                     /old_locus_tag="OIPHN260_53720"
                     /pseudo=""
     CDS             complement(18654..>18989)
                     /locus_tag="OIPHN260_RS26695"
                     /old_locus_tag="OIPHN260_53720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            19078..19455
                     /locus_tag="OIPHN260_RS26700"
                     /old_locus_tag="OIPHN260_53730"
     CDS             19078..19455
                     /locus_tag="OIPHN260_RS26700"
                     /old_locus_tag="OIPHN260_53730"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_244857424.1"
                     /translation="MQKNMTPGRRKGCPNYSPEFKQQLIAASCEPGISISKLALENGIN
                     ANLLFKWRQQWREGKLLLPSSESPQLLPVTLDATAVQPEPPAEDSELSISCEVTFRHGT
                     LRLNGTVSEKLLTLLIQELKR"
                     /name="transposase"
     gene            19452..19799
                     /gene="tnpB"
                     /locus_tag="OIPHN260_RS26705"
                     /old_locus_tag="OIPHN260_53740"
     CDS             19452..19799
                     /gene="tnpB"
                     /locus_tag="OIPHN260_RS26705"
                     /old_locus_tag="OIPHN260_53740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="WP_004114612.1"
                     /translation="MIPLPTGTKIWLVAGITDMRNGFNGLAAKVQTALKDEPMSGHVFI
                     FRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLLEGIDWRQ
                     PKRLLTSLTML"
                     /name="tnpB"
     gene            19849..21387
                     /locus_tag="OIPHN260_RS26710"
                     /old_locus_tag="OIPHN260_53750"
     CDS             19849..21387
                     /locus_tag="OIPHN260_RS26710"
                     /old_locus_tag="OIPHN260_53750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="WP_202324631.1"
                     /translation="MNDISSDDILLLKQRLAEQEALNRALLEKLADRERKIDHLQAQLD
                     KLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVDDPAVQRTLRQTRTRKPLPE
                     SLPRDEKRLRPAGSCCPECGGALSYLGEDAAEQLELMHSAFRVIRTVREKHACTKCDAI
                     VQAPAPSRPIELGIAGPGLMARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSGWVDA
                     CCRLLSPLEEALQDYVLTDGKLHADDTPVQVLLPGNKKTKTGRLWTYVRDDRNAGSALA
                     PAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHARRKIHD
                     VHVRTPSALTEEALKRIGELYAVEAEIRGMLAEQRLAERQRKTKPLLSTLESWLREKMK
                     TLSRHSELAKAFTYTLNQWPALTYYAENGWAEADNNIAENALRMVSLGRKNWLFFGSDH
                     GGERGALLYSLIGTCKLNGVDPESYLRHVLDVIADWPVNRVSELLPWRITLPTE"
                     /name="IS66 family transposase"
     gene            complement(<21430..21684)
                     /locus_tag="OIPHN260_RS26715"
                     /old_locus_tag="OIPHN260_53760"
                     /pseudo=""
     CDS             complement(<21430..21684)
                     /locus_tag="OIPHN260_RS26715"
                     /old_locus_tag="OIPHN260_53760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558270.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /name="IS1-like element transposase"
     Typing          complement(17649..17905)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="80.755"
                     /coverage="96.0"
                     /evalue="3.41e-47"
     Typing          3..136
                     /name="IncFIA"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.259"
                     /coverage="99.0"
                     /evalue="5.03e-62"
     Typing          22078..22328
                     /name="IncFIA"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="9.86e-129"
ORIGIN
        1 atgctgtatc tgttcgttga ccagatcaga aaatccgacg gctccctgca ggaacatgac
       61 ggcatctgcg aaattcacgt tgctaaatac gctgaaacat tcgggttgac ctccgctgaa
      121 gccagtaagg atatacgaca ggctttaaaa ggttttgcgg gtaaggaagt ggttttctat
      181 cgccctgaag aggatgccgg cgatgaaaaa gggtatgaat cctttccctg gtttattaaa
      241 cgtgcgcaca gcccatccag agggctttac agcgtacata tcaacccata tctgattccc
      301 ttcttcatcg ggttacagaa ccggtttacg cagttccggc tcagtgaaac aaaagagatt
      361 accaatccgt acgccatgcg tttatacgaa tctctgtgcc agtaccgtaa acctgatggc
      421 tcaggtgtcg tgtccctgaa aatcgactgg atcatggaac gctaccagct acctcaaagt
      481 taccagcgta tgccggactt tcgccgccgt ttcctgcagg caagtgttga cgagatcaac
      541 agccggacac caatgcgcct ttcttacatc gagaaaaaga aaggccgcca gacgacgcat
      601 atcgtatttt ccttccgtga tataacctcc atgacgattg aatagttgag ggttatctgt
      661 cacagattta agggtggttc gtcacatttg atctggaagg ttgagagtga tttgtcacag
      721 ttttgctgtt ccctccttgg catcctgcgc ttgctcatag tttttttggc tgtaatcggt
      781 agtggagcca gaattgaggg caatttgtca ccctatcctg agggtagttt gttacactag
      841 attttccctc tcttcccggg taatggtgcc aacttactga tttagtgtac gatggtgttt
      901 ttgaggtgct cccgtggcgg cactgttgca aatagtcggt ggtgataaac ttatcatccc
      961 cttttgctga tggagctgca catgaaccca ttcaaaggcc ggcattttca gcgtgacatc
     1021 attctgtggg ccgtacgctg gtactgcaaa tacggcatca gttaccgtga gctgcaggag
     1081 atgctggctg aacgcggagt gaatgtcgat cactccacga tttaccgctg ggttcagcgt
     1141 tatgcgcctg aaatggaaaa aacggctgcg ctggtactgg cgtaaccctt ccgatctttg
     1201 cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc cgctgggcgt atctgtaccg
     1261 ggccgtcgac agccggggcc gcactgtcga tttttatctc tcctcccgtc gtaacagcaa
     1321 agctgcatac cggtttctgg gtaaaatcct caacaacgtg aagaagtggc agatcccgcg
     1381 attcatcaac acggataaag cgcccgccta tggtcgcgcg cttgctctgc tcaaacgcga
     1441 aggccggtgc ccgtctgacg ttgaacaccg acagattaag taccggaaca acgtgattga
     1501 atgcgatcat ggcaaactga aacggataat cgacgccacg ctgggattta aatccatgaa
     1561 gacggcttac gccaccatca aaggtattga ggtgatgcgt gcactacgca aaggccaggc
     1621 ctcagcattt tattatggtg atcccctggg cgaaatgcgc ctggtaagca gagtttttga
     1681 aatgtaaggc ctttgaataa gacaaaaggc tgcctcatcg ctaactttgc aacagtgcta
     1741 tcagtaggct tttttatttt tattcccggc gtccagcgct ataatctgcg gacgattttc
     1801 tttgtggaat gtgcattgta gagcatgagt cgtttatccc acctgtcgga acatagcgcc
     1861 gccgccgtgt gcaaagggcc atcaagcctg aacgcgcgct gtctccctgg cggcgaagac
     1921 gatgtctctt cttctctttc cccgcatcgt gaacgctccc ttttgctgct ggctcacatc
     1981 ggcgggtcgc ccgttactct ttcatcggtg gcgaaaagcg cagacgttat accctgcgta
     2041 aatgagaaag tcctgatggt taatccggcg gcaaatgaag aaagtgtggt gcgtttggcg
     2101 gccaggtgag gaacagatgc atattaaaag cagagatatg tctcaaggca tatctctgct
     2161 tttagttttt caggcccccc gttctgtcag actggctttt caagccgctg agtgaacgat
     2221 ctgccgatag cctgaagcga aagcgggtgg cgtttagcct ggagcacggg agcagtacgc
     2281 attttggttg cgacagccaa tggcggtagc gtgcctgaaa aaggccgttc cacccagacg
     2341 gtcgattgat tgatagtaaa ataaagcaat tatttattta aggatttcat tatgttactt
     2401 cctgtaatta tggctggcgg taccggcagt cgtctctggc cgatgtctcg cgagctttat
     2461 ccgaaacagt tcctccgcct gttcgggcag aactccatgc tgcaggaaac catcacccga
     2521 ctctcgggcc ttgaaatcca tgaaccgatg gtcatctgta acgaagagca ccgcttcctg
     2581 gtggccgaac agctgcgcca gctcaacaag ctgtcgaaca acattattct tgagccggtt
     2641 gggcgcaaca ccgccccggc cattgctctc gcggccctcc aggccacccg ccacggcgac
     2701 gacccgctga tgctggtcct cgccgccgac catatcatca ataaccagcc ggtcttccac
     2761 gacgccatcc gcgtcgccga gcagtatgcc gatgaaggtc atctggtcac cttcggtatc
     2821 gtgccgaacg ccccggaaac cggctacggc tacatccagc gtggcgtggc cctcaccgac
     2881 agcgcccaca ccccgtacca ggtggcccgt ttcgtggaga agccggaccg cgagcgcgcc
     2941 gaggcctacc tcgcctccgg ggagtactac tggaacagcg gcatgtttat gttccgcgcc
     3001 aaaaaatacc tctccgagct ggccaaattc cgcccggata tcctcgaagc ctgccaggcc
     3061 gcggtcaatg ccgccgacaa cggcagcgac ttcatcagca ttccgcatga cattttctgc
     3121 gagtgcccgg acgagtccgt ggactacgcg gtgatggaga aaaccgccga cgcggtggtg
     3181 gtcggtctcg atgccgactg gagcgacgtc ggctcctggt ccgccctgtg ggaggtcagc
     3241 ccgaaagatg agcagggtaa cgtcctcagc ggcgacgcgt gggtgcacaa cagcgaaaac
     3301 tgctatatca acagcgacga gaagctggtg gcggccatcg gcgtggagaa cctggtgatt
     3361 gtcagcacca aggacgccgt gctggtgatg aatcgcgagc gttcccagga cgtgaagaag
     3421 gcggtcgagt tcctcaagca gaaccagcgc agcgagtaca agcgccaccg cgagatttac
     3481 cgtccctggg gccgctgcga cgtggtggtc cagaccccgc gcttcaacgt caaccgcatc
     3541 accgtgaaac cgggcggcgc cttctcgatg cagatgcacc accaccgtgc cgagcactgg
     3601 gtcattctcg ccggcaccgg ccaggtgacg gtcaacggca agcagttcct gctgactgag
     3661 aaccagtcca cctttattcc gattggcgcc gagcacagcc tggaaaaccc gggccgcatt
     3721 ccgctggaag tgctggagat ccagtcgggg tcgtacctcg gcgaggacga cattattcgt
     3781 attaaagacc agtatggtcg ttgctaattt ttcgggacaa aacgcagaat gacacagtta
     3841 acatgcttta aggcttatga catccgtggt gaactgggcg aggagctgaa cgaggacatc
     3901 gcctaccgta tcggccgcgc ctatggcgaa tttctgaaac ccgggaagat agtggtgggg
     3961 ggcgatgtgc gcctcaccag cgagtcgctg aagctggcgc tggcccgcgg gctgatggac
     4021 gccggcaccg acgtgctgga tatcggcctg agcggcacgg aagagattta cttcgccact
     4081 ttccacctcg gggtggacgg cggcatcgag gtgacggcga gccataaccc gatgaactac
     4141 aacggcatga agctggtgcg cgagaacgcg aagcccatca gcggcgacac cggcctgcgg
     4201 gatatccagc gcctggcgga ggagaaccag ttcccgccgg tggacccggc gcgtcgcggg
     4261 accctgcgcc agatatcggt gctgaaggag tacgtcgacc acctgatggg gtacgtggac
     4321 ctggcgaact tcacccgtcc gctgaagctg gtggtgaact ccggcaacgg ggcggcgggg
     4381 cacgtgattg atgaagtgga gaaacgcttc gcggcggccg gggcgccggt gacctttatc
     4441 aaggtgcatc accagccgga cggccatttc ccgaacggta tcccgaaccc gctgctgccg
     4501 gagtgccgcc aggacaccgc cgacgcggtg cgtgcgcatc aggcggacat gggtatcgcc
     4561 tttgacggcg acttcgaccg ctgcttcctg ttcgatgacg aggcgtcgtt tatcgagggg
     4621 tactacattg tcggcctgct ggcggaggcg ttcctgcaga aacagccggg ggcgaaaatc
     4681 attcacgacc cgcgtctgac atggaacacg gtggacatcg tgacccgcag cggcggccag
     4741 ccggtgatgt cgaagacggg gcatgcgttc atcaaggagc ggatgcgcca ggaagacgcc
     4801 atctacggcg gggagatgag cgcgcaccat tacttccgcg acttcgccta ctgcgacagc
     4861 gggatgatcc cgtggctgct ggtggcggag ctgctgtgcc tgaagaacag ttcgctgaaa
     4921 tcgctggtgg cggaccgcca ggtggcgttc ccggcgtcgg gagagatcaa ccgcaagctg
     4981 ggaaatgcgg cggaggcgat agcgcgcatc cgggcgcagt atgagcccgc ggccgcacac
     5041 atcgacacaa cggacggtat cagtattgaa taccctgagt ggcgctttaa cctgcgcacg
     5101 tccaacacgg agccggtggt gcgtctgaac gttgagtcca gagcggatac tgcgttaatg
     5161 aatgagaaaa ccgccgagct gctcaacctg ttaaaagagg aatcgctttg agtcctatgt
     5221 tttcagcgat ctatcgctac cgtggcttta ttattgacag cgtcaaacgg gactttcagt
     5281 cacgttacca gactagcttc ttaggcgcgg catggctgat cttacagccg atcgccatga
     5341 tttccgtata tacattaatc ttttctgagt taatgcgtgc ccgcctggcg ggcatggacg
     5401 gcccttttgc ctacagtatc tacctctgtt ccggggtgtt aacctggggg ctgtttacgg
     5461 aaacgctcaa tagtctggtc aacgtttttc tgaccaacgc caacattctt aaaaagctta
     5521 gctttccgcg gatctgttta ccaatcatcg tcactgcttc ggcgttcatt aacttcctga
     5581 tcatttttgg tctgttcgtg ctgtttctga tcgttacggg caatttcccg ggcatgattt
     5641 tctttgaaat cattccggtg ctgatcgttc agatgctgtt caccctcggc ctcgggatca
     5701 tcctcggggt gctgaacgtt tttgtccgcg acgtcgggca gttcgtgaat atcctgctgc
     5761 agttttggtt ctggtttacg cccattgtct acgtgtccaa aacgctgccg gagtgggtct
     5821 ctggtctgct ggcgtataac ccgatggcga ccattatcgg ttcataccag aacgtgatgc
     5881 tctatcacca gagccctaac tggctggcgc tgtttccggt cacggtgctg tccgtcattc
     5941 tgtttttatt tgcctggcgt ttatttaaaa aacatgccgc tgatattgtg gacgagattt
     6001 aatcatgagt atcaaagttc agcacgtcgg caaggcgtat aaatattatc catcaaaatg
     6061 gaaccgggtc attgagaaac ttctgccggg cgataagccg cggcacagca agaaatgggt
     6121 attgaaagat atcaatttca gtattgaacc cggtgaagcg gtcggcattg ttggggtgaa
     6181 cggcgcaggt aaaagtacgt tactgaagct gctgactggc accactcagc cgaccaaagg
     6241 cagcattgag atccaggggc gtgtcgctgc gctgctggag ctgggcatgg gattccatcc
     6301 tgactttacc ggtcggcaga acgtgtatat gtccgggctg atgatgggcc tgagccggga
     6361 agagattgag cgcttaatgc cggagatcga agcctttgcg gatatcggtg actacattga
     6421 agagcccgtg cgcatctact ccagcgggat gcaaatgcgc ctggcgtttg ccgtggccac
     6481 ggcttcccgc ccggatattc tgatcgtcga tgaggcgctc tcagtcggtg actcccgttt
     6541 tcaggcgaag tgctacgctc gaattgcgga ttttaaaaag cagggcacca cgctgctgct
     6601 ggtctcccat agcgccggtg atatagtcaa acactgcgac cgcgccattt tcctcaaaaa
     6661 tggtgatatc tgcatggacg gcaccgcccg cgatgtgact aaccgttatc tggatgaact
     6721 gtttggcaaa accgacaaaa acagcacgcc caaaagaaac ggcaaccttg tcagccagcg
     6781 gcgaaagcca gatgtcgctc gatgagattg aggatgtgta ccatacgcgg ccaggctatc
     6841 gtccggaaga atatcgttgg gggcagggag gggcgaaaat cattgattat cacatccaaa
     6901 gcgccggtat agattttcct ccatcgctga cgggcaatca gtataccgat ttcctgatga
     6961 aagtcgtgtt tgattatgac tttgattgcg tggtgccggg cattctgatt aagactctcg
     7021 atggtctatt cctctacgga actaactcct tcctcgcttc tgaagggcgg gagaatatct
     7081 cggtttcccg tggcgatgtt cgggtcttta aatttagcct tccggtggat ttaaacagtg
     7141 gcgattactt gctgtcgttt ggtatctctg ctggcaaccc gcagacggat atgacgccgc
     7201 tggacagacg ttacgactcc attattttac atgtggccaa gagcatggat ttctggggta
     7261 tcatcgatct taagtcgtcc tttactagct accaatgacg ttgagaaaaa caattccatg
     7321 actaaaaact taaaaacgct ggttagcgaa ttacctgaga tttatcaaac aatttttggc
     7381 catcccgagt gggatggcga cgctgctcgc gattgtaatc aacgtctcga tttaattacc
     7441 gaacagtacg acaacttatc ccgtgtgctg ggccgccagc ttaaagttct cgatctaggc
     7501 tgcgctcagg gttttttcag cctgagtctg gcaagcaaag gcgcgactat cgtcggtatt
     7561 gatttccagc aggaaaatat taacgtgtgc cgagcgctgg ctgaagaaaa ccccggcttt
     7621 gccgctgatt tccgggtagg cagaattgaa gaggtgatcg atgcgctgga ggagggcgag
     7681 tttgatctgg ccattggctt aagcgtgttt caccatattg ttcatcttca tggtatcgat
     7741 gaagtaaaac gcctgctctc gcgcttggcc gatgtcaccc aggcggtgat cctcgagctg
     7801 gcggtaaaag aagagccgct ctactggggc gtttctcagc cggacgatcc gcgtgaactg
     7861 attgagcagt gcgcgtttta tagactaatt ggtgagtttg atactcattt atcacaagtg
     7921 ccgcgtccga tgtacctggt cagcaaccat cgggtgctgc tcaacgattt taatcagccg
     7981 tttcagcact ggcaaaatca gccctatgcc ggggccggcc tggcgcataa gcgcagccgt
     8041 cgctacttct ttggcgaaga ttatgtgtgt aaatttttct attacgacat gccccacaac
     8101 accctgatgg cggaagagag tcagcgtaat aaaaacgaac tgcataatga aatgtgtttt
     8161 ctggcacaac cccctacggg ctttgacacg ccggcattac tagcccacgg cgaaaatgcc
     8221 cagtctggtt ggctggtgat ggaaaaactc ccgggtagct tgctgagcga tatgctggtg
     8281 gccggggaag agatcgatcg tgaaaaaatc attggcacct tgctccgttc cttagcggtg
     8341 ctggagaagc aagggttatg gcatgacgat gtgcgcccgt ggaacgtgat ggtcgatgca
     8401 cggcagcatg cccgcttaat cgactttggt tctatcgtca ccacgccaca ggactgcagc
     8461 tggccggcaa accttgtcca gtcgttcttc gtgtttgtga atgaactgtt tgctgaaaac
     8521 aaaaactgga ctggtttctg gcgctcggcg cccgtgcatc ctttcaacct gcctcagccg
     8581 tggtcaaact ggctgtatgc ggtctggcag gagccggttg gcagctggaa ctttgccctt
     8641 ctgttggcgc tgttcgacaa aaaagagaaa ctgccgtcgg ctgaacagca gcgaggggcg
     8701 acggagcagt ggattatcgc tcaggagacg gtgctgctgg agctccagtc tcgagtgcgt
     8761 aatgaaagtg ccagctcgga ggctatgcgt ggagaaatcc atgctcttga gcagcagatc
     8821 gttcagctgc aggctgaaca gcagcgaggg gcgacggagc agtggattat cgctcaggag
     8881 acggtgctgc tggagctcca gtctcgagtg cgtaatgaaa gtgccagctc ggaggctatg
     8941 cgtggagaaa tccatgctct tgagcagcag atcgttcagc tgcaggctga acagcagcga
     9001 ggggcgacgg agcagtggat tatcgctcag gagacggtgc tgctggagct ccagtctcga
     9061 gtgcgtaatg aaagtgccag ctcggaggct atgcgtggag aaatccatgc tcttgagcag
     9121 cagatcgttc agctgcaggc tgaacaggat gcgttagtgg aaaaggctgt gctgccagtg
     9181 tccatcagcc atgagctgag ctggctgact gagaatatgg agcagctgac cgcgctgctg
     9241 gggtcggcaa aagcgcaatc acaggctgaa attgagcccg agctgcctct ggaaaccgct
     9301 gagctactcc agcgactgga aaccgctaac cgggagattc accatttaaa taacgaaaac
     9361 cagcagcttc gccaggaaat tgaaaaaatc catcgcagcc gctcctggag aatgaccaag
     9421 ggttatcgtt atctgggatt gcagatccat ttgctaagac agtacggttt cgtgcagcgt
     9481 tgtaaacatt ttattaagcg tgtcctgcgg tttgttttct cgtttatgag aaaacatccg
     9541 aaggtaaaac atactgcggt aaatgggtta tataagctag gcctatatca gtcggcctac
     9601 cgtttgtatc gtcgtatgaa tccgcagccg tacagccagt atcaggctga tgcgcaaatt
     9661 ttatcgcaga ctgagctgca agtgatgcat ccggagcttt tacctcccgt ggtttatgaa
     9721 atttatctca aactaacgaa aaataaataa ggagattagc ttgcacattt tgattgatgt
     9781 ccagggttac cagtcggaaa gtaaattccg cggtatcggt cgcagtaccc tggccatgag
     9841 ccgcgcaatt attgaaaatg ctggtgagca tcgcgtcagt attctgatca atggtatgta
     9901 ctccattgag aatattaatg atgtcaaaat ggcctatcgg gacctgctta cggatgagga
     9961 tatgttcatc ttctctgccg tcgcgccaac ggcctactgc aatatcgata accacggccg
    10021 cagcaaagcc gcccaggcgg cccgcgatat cgctatcgcc aatatcgcgc ctgatattgt
    10081 ctatgttatt agcttcttcg aaggtcatgg agatagttat accgtgtcta tcccagcgga
    10141 cgacgtgccg tggaagacgg tatgcgtgtg ccacgatctg atcccgctgt taaataaaga
    10201 gcgttatctc ggcgacccga atttccgcga gttttatatg aacaagctgg cggagtttga
    10261 gcgggcggac gcgattttcg ctatttcgca atcggcggcc caggaagtga tcgaatatac
    10321 cgacatcccc agcgatcgag tgctgaacat ctcctcggcg gtgggagaag actttgcggt
    10381 gattgattat tccgctgagc atattcagtc tttaaaagac aaatacagac tgccggatga
    10441 atttatccta aggaaggtgc gaataagtca ccaaacataa aaatgcgcct ttgtaagtat
    10501 ttgaatttaa taaactaatt tttttcagtt tcgttgtgga gggtgcttat tcgcaccctc
    10561 cctaacgctg gcgatgattg agccacggaa aaacatcgaa gcgcttattc atgcttacag
    10621 catgctgcct gctgaattgc agcagcgcta tccgatggtg ctggcgtata aggtgcatcc
    10681 ggaggatttg gagcgtattc tgcgtctagc ggaaagctat ggcctatccc gcagccagct
    10741 tatctttact ggcttcctga ccgacgacga tctaattgcg ctgtacaacc tatgcaaact
    10801 gtttgtgttc ccgtcgctgc atgaaggttt cggcctgccg ccgctggaag cgatgcgctg
    10861 cggggctgcg accctgggtt caaatattac cagcttgccg gaagtcattg gctgggaaga
    10921 tgccatgttc aatccgcatg acgtgcagga tattcgccgg gtcatggaga aggcgctgac
    10981 cgatgaggcg ttttaccgtg agctgaaggc gcatgctctc actcagtcgg ccaaattctc
    11041 ctgggccaac accgcccatc tggcgatcga tggtttcacc cgcctgcttc agtcgtccaa
    11101 agaggtggat gccgggcaga cggaaggcgt gactacctcg cgcatccaga cgatgcagaa
    11161 aattgatgcg ctgagcgaag tcgaccgtct aggtctggcg tgggctgttg cgcgcaatgg
    11221 ttataaacag catacccgca agctgttggt ggacatttcg gttctggcta agcatgacgc
    11281 taagaccggg atccagcgcg tttcgcgcag tattcttagc gaattactga aatccggagt
    11341 accaggttac gaggtttccg ctgtctacta cacccctggc gagtgttatc gctacgcgaa
    11401 ccaatatctg tccagtaatt tccctggtga atatggtgcc gacgagccgg tcctgttcag
    11461 caaagacgat attctgatcg ccaccgacct gaccgcgcat ctcttcccgg aggtggtgac
    11521 acaaattgat tgcatgcgcg ccgcaggcgc attcgcctgc tttgtggtgc atgatattct
    11581 gccattacgc cgcccggagt ggagtatcga aggtattcag cgtgaattcc cgatctggct
    11641 gtcttgcctc gcagagcatg ccgaccgact gatctgcgtc tcagccagcg tcgccgagga
    11701 tgtgaaagcg tggattgcgg aaaacagcca ttgggtgaaa cctaatccgc tgctgactgt
    11761 cagcaacttc catctggggg ccgaccttga tgccagcgta ccgtccaccg gcatgccgga
    11821 taatgcacag gcgcttttag ccacgatggc cgcggcgcca tcatttatca tggtcggtac
    11881 catggagccg cgcaaaggtc atgcccagac gctggccgcc tttgaggaac tatggtgcga
    11941 gggcaaaaac tacaacttgt ttattgtcgg taaacagggc tggaacgttg acagtttgtg
    12001 cgaaaaatta cgccatcatc cccagctgaa caaaaagctg ttttggctgc aaaatatcag
    12061 cgacgagttt ttggtcgaac tatatgcgcg ttcacgtgcg ctgatctttg cctcgcaggg
    12121 ggagggcttt ggcctgccat tgattgaagc agcgcagaaa aagctaccgg tgattatccg
    12181 cgatatcccg gtgtttaaag agattgctca ggaacatgcg tggtatttct ccggggaagc
    12241 gccggccgac atagctaagg ccgtcgaaga ctggttagcc ttgtatgagc aaaacgctca
    12301 tcctcgttcc gaaaatatca actggttaac ttggaagcag agtgcggaac ttctcctgaa
    12361 aaatctgccg attatcgcgc cagccgcgaa gcaataatcg ctattaatag ggctaccccg
    12421 gaggcccttg tgatgagttc gttatgaaaa ttatttttgc tactgagcca attaaatacc
    12481 cattaacggg catcggtcgg tattccctgg agctggttaa gcggctggcg gtcgcccgcg
    12541 aaattgaaga attaaagcta tttcacggtg cgtcgtttat cgaacagatc cctttggtgg
    12601 agaataaaag cgataccaaa gccagcaatc atggtcgtct gtcggcgttt ctacgccgac
    12661 agacgctgtt gattgaggct tatcgcttgc tgcatccgcg gcgccaggcg tgggcattgc
    12721 gcgactataa ggattatatc taccatggcc ccaattttta tctgccgcat aaactggaac
    12781 gcgccgtgac cacgtttcat gacatatcca tttttacctg cccggaatat catccaaaag
    12841 atcgggttcg ctatatggag aagtccctgc atgagagtct ggattcggca aagctgatcc
    12901 tgaccgtttc tgatttctcg cgcagtgaaa ttatccgctt gttcaactat ccggaggagc
    12961 ggatcgtaac caccaagcta gggaaggtgc gaataagcgg ggaaattctt ctcggctgac
    13021 tcagtcattt catttcttca tgtttgagcc gattttttct cccgtaaatg ccttgaatca
    13081 gcctatttag accgtttctt cgccatttaa ggcgttatcc ccagttttta gtgggatctc
    13141 tcccactgac gtatcatttg gtccgcccga aacaggttgg ccagcgtgaa taacatcgcc
    13201 agttggttat cgtttttcag caaccccttg tatctggctt tcacgaagcc gaactgtcgc
    13261 ttgatgatgc gaaatgggtg ctccaccttg gcccggatgc tggctttcat gtattcgatg
    13321 ttgatggccg ttttgttctt gcgtggatgc tgtttcaagg ttcttacctt gccggggcgc
    13381 tcggcgatca gccagtccac atccacctcg gccagctcct cgcgctgtgg cgccccttgg
    13441 tagccggcat cggctgagac aaattgctcc tctccatgca gcagattacc cagctgattg
    13501 aggtcatgct cgttggccgc ggtggtgact aggctgtggg tcaggccact cttggcatcg
    13561 acaccaatgt gggccttcat gccaaagtgc cactgattgc ctttcttggt ctgatgcatc
    13621 tccggatcgc gttgctgctc tttgttcttg gtcgagctgg gtgcctcaat gatggtggca
    13681 tcgaccaaag tgccttgagt catcatgacg cctgcttcgg ccagccagcg attgatggtc
    13741 ttgaacaatt ggcgggccag ttgatgctgc tccagcaggt ggcggaaatt catgatggtg
    13801 gtgcggtccg gcaaggcgct atccagggat aaccgggcaa acagacgcat ggaggcgatt
    13861 tcgtacagag catcttccat cgcgccatcg ctcaggttgt accaatgctg catgcagtga
    13921 atgcgtagca tggtttccag cggataaggt cgccggccat taccagcctt ggggtaaaac
    13981 ggctcgatga cttccaccat gttttgccat ggcagaatct gctccatgcg ggacaagaaa
    14041 atctcttttc tggtctgacg gcgcttactg ctgaattcac tgtcggcgaa ggtaagttga
    14101 tgactcatga tgaaccctgt tccatggctc cagatgacaa acatgatctc atatcaggga
    14161 cttgttcgca ccttcccttg gtgttgcgtt ccgttgtccc tcagccccac catgacgact
    14221 gaagtggctg taaacgcagt acctatcaat ttcaatataa acaatgaact aacgctgtta
    14281 gcgtggtttt ttgcatcatt ggccctcaaa ccccagtaac ggttaaaaga aactttagtt
    14341 acaacacaca aggaatcggt tttacccata gcctttagcc ttagtgtcgt attgaacaat
    14401 cacacaagcc aaccagctag gacgagtaga cattttcttg tgtgttgtaa ctacttactc
    14461 aaagcacgtt tatcttccct cgattttaca gcactactaa ttgtccctcg actacaggcc
    14521 ataattttct gaacctggct ccaagaacta ccgctatcga gcaaccgatt aattgcatcg
    14581 tatcgagcct gattgacctg acgcccctta tattttccct ccttttgagc tttattaatc
    14641 ccctgccttt gacgttcccg gcgttgttca taatcacgac gagccacagc agccagcata
    14701 tcaagcaaca tatcgttgat tgcagcaaac atgcgactat caaaatcatg gctcgctgaa
    14761 gcaatccagg ttgtcggtac gttgatggcc atcactcgaa tatccttctg gcgaatcaac
    14821 ttcttcaggc tgttccagtc ttcaccagtt aagcgtgaaa gccgatcaac atcttcaatg
    14881 aggagaatat cgttctgtcg gcagtcgttc agcagtctaa ataattccgg tcggtcgagc
    14941 cgcgaaccag attcattctc gatgtagtaa ttgcagatgt taaggccacg ctcatcagca
    15001 aagctatcga tcgcagcgag tgcgcgagaa gcgtcttgtt cagatgttga ggcgcgtaaa
    15061 taagcccgga caaagctttg cggtacggta tgagtcacaa tgtcgaacca gttcaattta
    15121 ggttattaaa gcataaccat atagtacctg tttcgcatgg tattttaaag gcatactcta
    15181 aaagaaccat taagaactta atgtgacaac gtaacatgga tactttcaca acataaagac
    15241 aatgtaaaga caaaataaag acaaactaat ctttataagt gctttacaaa gtaaagttaa
    15301 tgcatggtaa agacagtttt atctttacac atggtatacg atgtaaagac aatgttatct
    15361 ttacacaagg tagacattgt aaagaaacag ccgtctttac aatggcttta caatataaag
    15421 agaggaagtc tgaaatgggt aaaattttgc ttgttgtatc agataagggc ggggtaggga
    15481 aaagcaccta tgtagcgaat actggcgcaa tgctagttaa caaaggaaag tcggtcatta
    15541 tcttgaagac cgataagaat cccgacctgt tgagctggaa tgagaagcgg atcgataacg
    15601 gattgctcac gattccggtt cacgagtcat atggcaacgt cagcaacgaa attaagcgcc
    15661 ttagcaagct ttgtgaggtt ctcattgtcg attgtccagg ccatgacagc caagagtttc
    15721 gtagcgctct aacagtgtcc gacattgtgg taacgcttgt taagccgtcc tctgatttcg
    15781 aaagtgaaac cctgaccagt gttacggaga aagttcgtac tgctcagaaa actaactcag
    15841 ccctagaacc gtgggtgtta ttcaccagga tcaactcagc gaaaccacgt cacagaaaag
    15901 cggctattga gctagataaa ttactgcgtt cggaaaacat ctggattcaa ccactgaaaa
    15961 cccgcatatc cgaactggac gtttatgaaa gcgcctgtaa tgaaggggca ggggtgcatg
    16021 atgtaagtcg ggcatcgagc ctttcaacag ctaaagcaca gattgaactt gtcgcacagg
    16081 aaatagggat tttgtaaata tatcaatgat atgaattcac atctttacat tgtaaagata
    16141 ctataaagac agtatgatat ttacagattt tgcatcccgt acaagaggaa attttaatgg
    16201 ctttaaaact gaaccgcccg aacgtcgaag aatctcagca gccagcaacc agcgcggaaa
    16261 ccgcgcgctt tatatccggc gcaaccagaa cgccattagt ggggaaacca aagctgatga
    16321 acttcagact accagggcac tttgaggaga ttctggagtc agagtcgcag agaacagggc
    16381 agagcaagac aaccgttcta aaggcagcgc tggccgcttt tgataatctg gatgagaacc
    16441 agaaaaacca ctggttactt gagtcagcaa aattaggtta aaaagccgtt gcctatgaca
    16501 gcggagaatg gattgtcaaa ccctcgtatt tgttgagaat tgaaacttca aaatattaag
    16561 aaaaaaaagg tttgagatat gttcacatgc agatataggt gatgctgcca acttactgat
    16621 ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct
    16681 cctgttcagc tactgacggg gtggtgcgta acggcaaaag cactgccgga catcagcgct
    16741 atctctgctc tcactgccgt aaaacatggc aactgcagtt cacttacacc gcttctcaac
    16801 ccggtacgca ccagaaaatc attgatatgg ccatgaatgg cgttggatgc cgggcaactg
    16861 cccgcattat gggcgttggc ctcaacacga ttttacgtca cttaaaaaac tcaggccgca
    16921 gtcggtaacc tcgcgcatac agccgggcag tgacgtcatc gtctgcgcgg aaatggacga
    16981 acagtggggc tatgtcgggg ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag
    17041 gctccggaag acggtttgcg cacgtattcg gtgaacgcac tatggcgacg ctggggcgtc
    17101 ttatgagcct gctgtcaccc tttgacgtgg tgatatggat gacggatggc tggccgctgt
    17161 atgaatcccg cctgaaggga aagctgcacg taatcagcaa gcgatatacg cagcgaattg
    17221 agcggcataa cctgaatctg aggcagcacc tggcacggct gggacggaag tcgctgtcgt
    17281 tctcaaaatc ggtggagctg catgacaaag tcatcgggca ttatctgaac ataaaacact
    17341 atcaacaagt tggagtcatt atccatcgcg cagtgagcca aaatccgcat ctgaagcacg
    17401 gtcaaaaagg taatccagtt tgcgatttac gtcgctcagc tggttggtaa ctattttcaa
    17461 cccggactcc agatcacctg ggccagcttc aaggctgcgc cacagatact ctgaccgatt
    17521 accacctgaa accaggtcta tataggccaa aagttcatct gatacttttg cggttattgt
    17581 tggcattcag tcctcacttt gtgcattttt taaacgaaaa aagggtgtct aatacggtga
    17641 aatcatagta ttaccaaagt aataaataaa aaatgtttta aatcagtaag ttagattaga
    17701 tttgtaatac ctgggtaata ccgcagcatg gctaaatttt atttttttct tttaaataag
    17761 tgagatagcg tgatttatca cgctgcgtta ggtgtatagc aggttaaggg ataaaaaatc
    17821 atcttttttt ggtagggcga tctccgtagg ttaagggtca tttggctaaa aagcgtccta
    17881 ttctttgatg gtcatgcttg catgaccatc tgagcaacca aaactacaga taaactacaa
    17941 aaaacgtttt gccttagtgt ttgcagacta caaaaagact acaaagaaac tacaaaaaca
    18001 gtgacagact acaaagagac tacaagaaaa ctacagataa actacaaaac tcgattgacc
    18061 ccttcttacg agtgttgtag agtcattttc atacaacgga gggggttatg aataaaccag
    18121 cagatctgaa accccgcaac ttatcggctg ctgtcagatt gcgcctaaat gaaatcgaga
    18181 actggctgga cagagggcta acgcggcatg aaattgctga aatcctcgac agcgaataca
    18241 gcttttcggt aacagccaaa gggcttgaga tggcactgta tagaacgcgg aaaaaccgaa
    18301 aaaatgtatt gtacaataca caaccgcctg agcctgaagc gcaggaaagt gaaaaagcag
    18361 agagtcccgg cattattgat aaagaattct tcaataaaat cggtgaggat ttcaacccta
    18421 agaagttcaa caaaaaattc tgaggtgatt tatgaaagta gcggtaatta attacagtgg
    18481 cagtgttggt aaaaccttaa tttcatctta cctgttagcc ccgcgcctga ctggtgcaaa
    18541 gttctatgcg gtagagacta tcaaccagtc tgcttccgat ctgggtattg aaaatgtgac
    18601 cagttttaaa ggtgacgact tctcacgttt gattgagggg taatgactcc aacttactgg
    18661 tagtgtttta tgttcagata atgtccaatg actttgtcat gcagttctcc cttcaggcgt
    18721 gattcataaa gcggccagtc atccatcatc cataccacga cctcaaaggc cgacagcagg
    18781 cccggaagac gctccagcgt ggccaacgtg cgttcaccga atacgtgcgc cacaaccgtc
    18841 ctccgtatcc tgtcatacgc gtaaaacaac cagcgctggc gtgatttagc gccgacgtaa
    18901 ccccactgtt cgtccatttc cgcgcaaaca atgacgtcac tgcccggttg tatacgtgag
    18961 tttaccgact gcggcctgag tttttgtaag cgtacagcgt gaaccgtctg gtcataatct
    19021 gaagcatccg acaaagtggt gtccaccaaa taagtagtgg gaaccaaagt gtcagatatg
    19081 cagaaaaata tgactcccgg caggcgtaaa ggctgcccta attattctcc tgagtttaaa
    19141 cagcaactca ttgctgcctc ctgcgaaccc gggatatcca tctcaaaact ggcgcttgaa
    19201 aatggcatta atgccaatct gctctttaaa tggcgccagc agtggcgcga gggaaagctg
    19261 ctattacctt cctctgagag tcctcagtta cttcctgtga ctctcgatgc caccgccgta
    19321 caaccagaac cgcccgctga ggactcagag ctcagcatca gctgtgaggt aacgttccgg
    19381 cacgggacac tccgcctcaa cggcactgtc agcgaaaagc ttctgactct gctgatacag
    19441 gaactgaagc gatgatcccg ctaccaacag gtaccaaaat ctggctggtt gccggtatta
    19501 ccgatatgag aaacggcttc aacgggctgg ctgcaaaagt gcagacggcg ctgaaagacg
    19561 aaccgatgtc cggccatgtc ttcatcttcc ggggccgcag cggcagtcag gttaaacttc
    19621 tgtggtccac cggcgacggg ctgtgcctgc tgaccaaacg gctggagcgc gggcgcttcg
    19681 cctggccgtc agctcgcgat ggcaaagtgt tcctgacccc ggcgcagctg gcaatgctgc
    19741 tggaaggcat cgactggcga cagccgaagc ggttgctgac ctccctgacc atgctgtagg
    19801 cttctttatc ctggttgtcg cagaataagc ctggtaaaat gctggcttat gaatgacatc
    19861 tcttctgacg acatccttct gctgaaacag cgccttgccg aacaggaagc gctgaaccgt
    19921 gccctgctgg aaaagctggc agaccgggag cgcaaaatag accatctgca ggcgcagctg
    19981 gataaacttc gccggatgaa cttcggcagc cgttccgaaa aagtctcccg ccgtatcgca
    20041 caaatggaag ccgatctgaa ccggcttcag aaagagagcg atacgctgac cggtcgggtg
    20101 gatgacccgg cagtacagcg cactctgcgt cagacccgca cccgcaaacc gctccccgaa
    20161 tcactccccc gcgatgaaaa acgcctgcgg ccagcgggtt cctgctgccc ggagtgtggt
    20221 ggtgcgctga gttacctggg tgaggatgcc gctgaacagc tggagctgat gcacagcgcc
    20281 ttccgggtga tccggaccgt acgggaaaaa catgcctgca caaaatgtga tgccatcgtg
    20341 caggcccccg cgccttcgcg ccccattgag ctgggtatcg ccggaccggg gctgatggcc
    20401 cgcgtgctga cctcaaagta tgcagagcac acaccgctgt atcgtcagtc tgaaatatac
    20461 ggtcgccagg gcgtggaact gagccgttca ctgctgtcgg gctgggtgga tgcatgctgc
    20521 cggctgctgt ccccgctgga ggaggcgctt caggactatg tcctgactga cggtaaactc
    20581 catgccgatg ataccccggt ccaggtgctg ttgccgggca ataagaagac gaagaccggg
    20641 cggttgtgga cgtatgttcg tgatgaccgc aacgccgggt cagcgctggc accggcggtg
    20701 tggttcgcct acagccccga cagaaaaggc atccatccgc agacccatct tgcggggttc
    20761 agcggtgtgc tacaggcgga tgcgtacgcc gggttcaacg agctgtaccg cgatggccgg
    20821 ataacggaag ccgcctgctg ggctcacgcc cgccgtaaaa tccacgatgt gcacgttcgc
    20881 acgccgtcag ccctgacgga ggaagccctg aaacgtatcg gcgaactgta tgccgtggag
    20941 gcggaaataa gaggaatgct ggcggaacaa cggcttgctg aacgtcagcg gaaaacgaaa
    21001 ccgctgctta gtaccctgga aagctggttg cgtgaaaaaa tgaaaacgct gtcgcgacac
    21061 tcggagctgg caaaggcgtt cacgtacacc ctgaaccagt ggccggccct gacgtactat
    21121 gcagaaaacg gctgggccga agccgataat aacatcgctg aaaatgcgct gcggatggtc
    21181 agtctgggtc gtaaaaactg gttgttcttc ggctcagacc acggtggtga gcggggagcg
    21241 ttgctgtaca gtctgatcgg gacgtgcaaa ctgaacggcg tggatccaga aagctacctt
    21301 cgccatgtcc ttgacgtcat agctgactgg ccggtcaacc gggtcagcga gctgctaccc
    21361 tggcgcatca cactgccaac tgaataacac atccccgtca atacggttct cgctgtacgc
    21421 ttacgagttt tttaagtgtc gtaaaatcgt gttgaggcca atgcccataa tgcgtgcact
    21481 ggcgcgacat ccgacgccat tcatggccat atcaataatt ttctggtgtg taccgggctg
    21541 agaggcggtg taagtgaact gtagctgcca tgttttacgg cagtgagagc agagatagcg
    21601 ctgatgtccg gcagtacttt taccgttacg caccacgcct tcagtagctg agcaggaggg
    21661 acaactgatg gagatggaag ccacgggagc acctcaaaaa caccatcgta cactaaatca
    21721 gtaagttggt agcattaccc agaactggac agaataggct gtatgtctaa cttctggcca
    21781 cagatataat ctagccagca gtgctcgccg cagccgagcg acagggcgaa gccccgagtg
    21841 agcgaggaag caccatggaa cagtacttat aaattctgca tacgctcaat gcctgcaaaa
    21901 tcatctcccc taggggttat ccacttatcc acggggatat ttttataaga tctttttaaa
    21961 tagttgttag atctttattt ttagaatgcc cacaggcctt tatccatgcg ggttctagag
    22021 agggatctgt gacaaaacgc cctttcagtg tgacaaatca ccctcaaata gccgcccttt
    22081 ctgtgacaag ttgcccttaa ccctgtgaca aattgccctc aggaagcgtt gcttttcaca
    22141 aagttatcca tccctgttga ctctgtttta tgaagtgtga caatcttaaa gtctgtcaca
    22201 cttcacatgg acctgtcatg gcggaaatag cggttataaa ccataaaaaa cgtaaaaata
    22261 gcccgcggat tgtccagtca aatgagctga ctgaggcggc atatagtctc tccagggatc
    22321 aaaagcgt
//
