LOCUS       NZ_AP023437             2287 bp    DNA     circular CON 07-JUN-2024
DEFINITION  Escherichia coli strain M719 plasmid pM71904, complete sequence.
ACCESSION   NZ_AP023437
VERSION     NZ_AP023437.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00231967
            Sequence Read Archive: DRR233591, DRR233592
            Assembly: GCF_023167605.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Sakamoto,N., Akeda,Y., Sugawara,Y., Takeuchi,D., Hagiya,H.,
            Matsumoto,Y., Motooka,D., Zin,K.N., Aye,M.M., Tomono,K. and
            Hamada,S.
  TITLE     Expression of carbapenem resistance in enterobacteriaceae with
            chromosome/plasmid located blaNDM
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Sakamoto,N., Akeda,Y., Sugawara,Y., Takeuchi,D. and Hamada,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Contact:Noriko Sakamoto Osaka University,
            research Institute for Microbial diseases; 3-1 Yamadaoka, Suita,
            Osaka 565-0871, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.7
            Sequencing Technology             :: minION; Illumina HiSeq 3000
            Genome Coverage                   :: 349x
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023167605.1-RS_2024_06_07
            Annotation Date                   :: 06/07/2024 02:48:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,134
            CDSs (total)                      :: 5,009
            Genes (coding)                    :: 4,706
            CDSs (with protein)               :: 4,706
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 137 of 303
            Pseudo Genes (incomplete)         :: 183 of 303
            Pseudo Genes (internal stop)      :: 59 of 303
            Pseudo Genes (multiple problems)  :: 67 of 303
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023437.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="M719"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pM71904"
                     /geo_loc_name="Myanmar:Yangon"
                     /lat_lon="16.7788 N 96.1490 E"
                     /collection_date="2017-02-06"
     gene            1..966
                     /locus_tag="M2043_RS25505"
                     /old_locus_tag="MYEC719_p40001"
     CDS             1..966
                     /locus_tag="M2043_RS25505"
                     /old_locus_tag="MYEC719_p40001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            970..1236
                     /locus_tag="M2043_RS25510"
                     /old_locus_tag="MYEC719_p40002"
     CDS             970..1236
                     /locus_tag="M2043_RS25510"
                     /old_locus_tag="MYEC719_p40002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          395..626
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 atgtcagaag ataaattcct ttcggactac agcccccgtg atgcagtttg ggatacccag
       61 cgcacgctta ccgattctgt cgggggtatc taccagactg ctgctgaatt cgagcgctat
      121 gcactccgta tggcctcctg tagcggtttg ttacgttttg gttggtctac catcatggaa
      181 accggagaaa cgcgcctacg gcttcgtagt gcgcaatttt gccgtgtccg tcattgccct
      241 gtctgccagt ggagaagaac cctcatgtgg caagcccgtt tttatcaggc tctaccgaaa
      301 atcgttgtgg attacccgtc ttcccgatgg ttgtttctga cgttaactgt caggaactgc
      361 gagataggtg aacttggaac agtccttaca gcaatgaatg cggcgtttaa gcgaatggaa
      421 aagcgaaagg agctatcacc tgttcagggg tggatcaggg ctacggaggt gacgcgaggt
      481 aaggatggca gcgcacatcc gcattttcac tgtctgctga tggtgcaacc ttcttggttt
      541 aaagggaaga actacgttaa gcacgaacgt tgggtagaac tctggcgcga ttgcttgcgg
      601 gtgaactatg agccgaatat cgatattcgg gcagtaaaaa ctaagacagg tgaggttgtg
      661 gccaacgttg ccgagcaact gcaaagcgcg gttgctgaaa cgctgaaata ctccgttaaa
      721 ccggaagata tggcaaacga tcctgagtgg tttcttgagc tgacgcggca gcttcacaag
      781 cgccgtttta tctcgaccgg tggggcgcta aaaaacgtcc tccagttgga tcgagaaacc
      841 aatgaggatc ttgtcattgc cgacgatgta ggggatggca ctgatgacgg gaagcggacg
      901 gcgtttgtct gggattcagg taaacggcgt tacaaacgcg cccctgagaa ggataaatcg
      961 gattaacgta tgaatattaa tattgaatac ctgaatggaa ataagactat tggtttattt
     1021 tttttaagaa gtgaagcggt gattcctgac aggtttaaaa accttatttt gcttattgat
     1081 ggattaagtt ttggcacatt tggttttcat ccgcacgaag gttttgagga tgaattaatt
     1141 ttatatattc agaaaacaaa cgagagggta aaaactcttt ttgtgaaaat agatttaaac
     1201 ggtatcaagt ttggattttt aagaacgcat tcttagttct ggaaaagagc cagcggcagg
     1261 ctgaggtgat aggtacgaga ttgcatgcaa tctctagtgc tctgtctatc ctgcattatc
     1321 ctcagcatta tcctcagcat tatcctcagc cttgccaact cgacaccaat gcaggataga
     1381 caatccgatg tcaaatgtta acactctgcg agtggtacat tttccccgga ttatcgtcct
     1441 gagcctgccg ctggctctct ttctaccgcc tcgctttgct cgttgctcaa cgcctcacag
     1501 acacggatta aaatccgcat ccgttcaccg ttttttaaag tccgttaaaa gcatgatgcc
     1561 atctccgaga gttagtcaaa tgaaatgcaa atcggattaa ccttagcaat acgtaactga
     1621 acgaagtagg cccaacgaag tgggttttga caggtcaaag aaaatggagc agaattgagg
     1681 cgttttaatc ggcgttgggg agtgcgtgct caccgttctg ttaaagtccg ttaaaagcat
     1741 gatgccatct ccgagagtta atctcgtcaa atgctaaatc gtgggggtcc cctttggggt
     1801 tccgatttag tgattgacga caccaccgat taaaaaactt atgcggggtg gatggtttca
     1861 cgaagtgagg ccatccacct gtaagacagg gttttgtttt tattccctgt tttggtgatc
     1921 gggtgtgtgg aaaaggttgg ggtaagccgt tcgggggtgc ttgttttggg gggttaaaat
     1981 tgtggttatt ttttgcgcaa ttctcgcgcg tgatccttgt atttatactt aagggataaa
     2041 tggcggatat gaaatagtgg tttagcccag taatgacgag gctttgagtg ggttttgaca
     2101 ggtcaaagaa aatggagcag aattgaggcg tttttaatcg gcgttgggga gtgcgtcaac
     2161 actccccaac atttcgaatg tgtcacctca gcggcaaact ctggtgacat gtactggctc
     2221 gcaatgcaca ggtacgtgat gaatatacca catcaaatca cagcctgccc agatcggagc
     2281 aggctta
//
