LOCUS       NZ_AP023407             2624 bp    DNA     circular CON 08-FEB-2024
DEFINITION  Aeromonas hydrophila strain KAM330 plasmid pKAM330_9, complete
            sequence.
ACCESSION   NZ_AP023407
VERSION     NZ_AP023407.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00242552
            Assembly: GCF_014679585.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas hydrophila
  ORGANISM  Aeromonas hydrophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Suzuki,M., Maehana,S. and Kitasato,H.
  TITLE     Whole genome sequencing of carbapenem-resistant Aeromonas spp.
            isolated in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Suzuki,M., Maehana,S. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2020) Contact:Masato Suzuki National Institute of
            Infectious Diseases, AMR Research Center; 4-2-1 Aobacho,
            Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.4.8.0; Canu v. 1.8
            Genome Coverage                   :: 537x
            Sequencing Technology             :: Illumina MiniSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/08/2024 03:17:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,147
            CDSs (total)                      :: 4,982
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 165
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 126
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 44 of 114
            Pseudo Genes (incomplete)         :: 73 of 114
            Pseudo Genes (internal stop)      :: 12 of 114
            Pseudo Genes (multiple problems)  :: 12 of 114
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023407.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Aeromonas hydrophila"
                     /mol_type="genomic DNA"
                     /strain="KAM330"
                     /db_xref="taxon:644"
                     /plasmid="pKAM330_9"
                     /geo_loc_name="Japan:Kanagawa"
                     /collection_date="2018"
     gene            join(2558..2624,1..293)
                     /locus_tag="IFA22_RS25605"
     CDS             join(2558..2624,1..293)
                     /locus_tag="IFA22_RS25605"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_232105325.1"
                     /translation="MTFLSAQPELAFTVTVTHIIYRYSDKKGVAMKDQHDTQTADLLPI
                     KRPRGRPRTGKAMTQAERQAKYRAKLAENNVTVTVNRRLLERLDAQMQALRDGSSAQIL
                     TPDEAGQVLEALRRA"
                     /name="hypothetical protein"
     gene            941..1795
                     /locus_tag="IFA22_RS25610"
                     /old_locus_tag="KAM330_50350"
     CDS             941..1795
                     /locus_tag="IFA22_RS25610"
                     /old_locus_tag="KAM330_50350"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_190284541.1"
                     /translation="MRWLKGKYGDNLVSVIEHQDEAHPHLHFYAVPAPGQTFDDIHQGR
                     AAAATAKRAGERVCIQRAAHGKAMKVWQDELYQGVSRELGLMRLGPKRLRIPNRREWLS
                     HQTTMREMLNEERRFRDELLERESKLQGKEQEHSTRERSLAGREAALDTRSAKIAADIR
                     GLNDLKGEIDDKFSKLSTQVELERERALAERGRLGVLEAELNEREAKLNEREAKLNERE
                     AEIEAKTPASIKEQNIAFKGLIHAIVARLPEAQLQKLGQTMNRDHRQHLMMVMTSQGRD
                     YNR"
                     /name="hypothetical protein"
     gene            complement(1792..2316)
                     /locus_tag="IFA22_RS25615"
                     /old_locus_tag="KAM330_50360"
     CDS             complement(1792..2316)
                     /locus_tag="IFA22_RS25615"
                     /old_locus_tag="KAM330_50360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271729.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_190284540.1"
                     /translation="MSSDSSRNETPQSHQNHNSNFVQLSRAYLAQMRNLARKNPVAHEI
                     LYYLVENMGRTTNAIVCSYKTLCEVTGVSRPTVGRAIKTLKDDNWVDVVKIGNASAYCV
                     NERVFWQAGRNQRQYAVFSATVIAAASDQDSDFHNKAKHKLTYIPVIRDNDIPVTGTDQ
                     LPPPDQQDLDL"
                     /name="replication/maintenance protein RepL"
     Typing          25..304
                     /name="rep_cluster_2003"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.833"
                     /coverage="95.0"
                     /evalue="2.82e-83"
ORIGIN
        1 acagtgacaa aaaaggggtt gccatgaaag accagcacga cacccaaacc gccgatctgc
       61 tccccatcaa gcgcccacgg ggcagacccc gcaccggcaa ggccatgacg caggccgaac
      121 gccaagccaa gtaccgcgcc aagttggcag agaataacgt cactgttacg gttaatcgca
      181 ggcttcttga gcggttggat gcccagatgc aggcgctcag ggatggcagt agcgcccaga
      241 tcctcacgcc agacgaagcg gggcaggttc tggaggctct ccgccgtgca tagatggttc
      301 agctcaaagg acaggggcgg tagcccctac aagccgccca agggggtttc tggtggtctc
      361 tgggggtagg cggcgggaca tgcccatgcc tacctgtggc agtgcccccc ccttccctaa
      421 aaacaggctt ttgaggggtg cagcgagtga gggaggggaa cgccgcagcg cccccagcac
      481 tgtcagttgt cacatgttta gccccgcagg gccgacactt atcacctccc cagcgaagcg
      541 gggaaggtga taagtaggag tcggttactg ttacttatca acgacctcaa ggaatgtgca
      601 ataattgggc tctgattcag caggtaaggg ggggggtatg taccagttcg ggcacgtcga
      661 aagctacgcg atcaagccgt caaagggaca tggcatagag gggatctgga aggaggcaga
      721 acgagtggat ggctactgcg gccatgtgga aaatccacag ccacctgtgc aggtctacgg
      781 gtgcagccca agggaggcag ctaatagggc cgcgaattgg gcagggcagg ccaaagacgc
      841 gaaggggcgc aaacttcgct gtgatgcccc ctgtttgctt gctggtgtgc tttcctaccc
      901 tcgggcaggg gatgactggg atagcttcaa ggaggcgtct gtgcgctggc tgaagggaaa
      961 atacggcgac aacctcgtat cggtcatcga gcaccaagac gaagcgcacc cacatctgca
     1021 tttctacgcc gtgcctgcac cagggcagac gtttgacgat atccaccagg gcagggccgc
     1081 agccgccacg gccaagcgcg caggcgagcg ggtatgcatc cagagagcag ctcacggtaa
     1141 agcaatgaaa gtgtggcaag acgagctgta tcagggggtt tcgcgggagc tgggacttat
     1201 gcgacttggg ccgaagcgcc tccgtatccc caataggcga gagtggttgt cgcatcagac
     1261 cactatgcgg gagatgctta acgaagagcg gcggtttcgg gatgagctgc ttgaacgcga
     1321 gagcaagcta caggggaaag agcaggagca ctcgacacgg gaaaggagcc ttgcagggag
     1381 agaggccgcg cttgatacaa gatccgcgaa gatcgctgcc gatatacgcg ggctcaatga
     1441 tctaaaaggc gaaatagacg acaagttctc aaagctttcc acccaagtag agctggagcg
     1501 agagcgagcc cttgcagaga gagggcgctt aggagtattg gaagccgagc tgaatgaacg
     1561 ggaggccaag ctgaatgaac gggaggccaa gctgaatgaa cgggaggccg agatcgaggc
     1621 gaaaacccct gccagcatca aagagcaaaa catagcgttc aaggggctga tccatgcaat
     1681 agtcgcccgc ctcccagagg ctcagctaca aaaactcggg cagaccatga accgagacca
     1741 tcgacagcat ttgatgatgg tcatgacatc acaggggcgg gactataacc gctagaggtc
     1801 tagatcctgc tggtctggcg gaggtagctg atcagttccg gtgacgggta tatcgttatc
     1861 tctgatcacc ggaatgtagg tcagcttgtg cttggcttta ttgtgaaaat cgctgtcctg
     1921 atctgatgct gctgcaatga ccgtggcaga gaacaccgca tactgacgct ggtttcgccc
     1981 tgcctgccag aacactcgtt cgttgacaca gtaggcggaa gcattgccga tcttcacgac
     2041 atcaacccag ttgtcatcct tgagagtctt gatcgctctg ccgactgtcg gcctactcac
     2101 tccagtaacc tcgcataggg tcttgtaact gcatacgatg gcattcgtgg ttcgccccat
     2161 gttctccact aggtagtaca ggatctcgtg cgctacgggg tttttacggg ccaaatttcg
     2221 catctgcgcg agataggccc gcgagagctg cacgaaattg gagttgtggt tttggtgaga
     2281 ctgaggggtc tcattacgtg agctatccga actcatatta tgatgcaacc taactcatga
     2341 gatgatgcaa cactattgca cgggtgagac tggttgaaaa ccatagtctc acccggtgag
     2401 actgggtagt ctcaccgggt gagactaccc ccccaagtaa ctcgttgttt ttaaagtaaa
     2461 tcacaaattc ccctttctat ctctgattag gccccctccg tgccccacaa ctgccgcgct
     2521 atcgcttgcc tgctggcagg gtcgtcaggt cgaactgatg accttcctct ctgcccagcc
     2581 tgagcttgca tttaccgtca cagtgacgca tattatttac cgtt
//
