LOCUS       NZ_AP023406             4897 bp    DNA     circular CON 08-FEB-2024
DEFINITION  Aeromonas hydrophila strain KAM330 plasmid pKAM330_8, complete
            sequence.
ACCESSION   NZ_AP023406
VERSION     NZ_AP023406.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00242552
            Assembly: GCF_014679585.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas hydrophila
  ORGANISM  Aeromonas hydrophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Suzuki,M., Maehana,S. and Kitasato,H.
  TITLE     Whole genome sequencing of carbapenem-resistant Aeromonas spp.
            isolated in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Suzuki,M., Maehana,S. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2020) Contact:Masato Suzuki National Institute of
            Infectious Diseases, AMR Research Center; 4-2-1 Aobacho,
            Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.4.8.0; Canu v. 1.8
            Genome Coverage                   :: 537x
            Sequencing Technology             :: Illumina MiniSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/08/2024 03:17:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,147
            CDSs (total)                      :: 4,982
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 165
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 126
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 44 of 114
            Pseudo Genes (incomplete)         :: 73 of 114
            Pseudo Genes (internal stop)      :: 12 of 114
            Pseudo Genes (multiple problems)  :: 12 of 114
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023406.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Aeromonas hydrophila"
                     /mol_type="genomic DNA"
                     /strain="KAM330"
                     /db_xref="taxon:644"
                     /plasmid="pKAM330_8"
                     /geo_loc_name="Japan:Kanagawa"
                     /collection_date="2018"
     gene            join(4737..4897,1..148)
                     /locus_tag="IFA22_RS25570"
     CDS             join(4737..4897,1..148)
                     /locus_tag="IFA22_RS25570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="WP_094965908.1"
                     /translation="MQFTIYRNKGNARAYPYLLDVQSDIIGELATRIVIPLFPLAAFTG
                     RPAQRLNPVIIVEGDKYLVMTQDMAAVRQTQLGEEVTGVQESRQLIKDAIDFLMDGV"
                     /name="CcdB family protein"
     gene            263..694
                     /locus_tag="IFA22_RS25575"
                     /old_locus_tag="KAM330_50270"
     CDS             263..694
                     /locus_tag="IFA22_RS25575"
                     /old_locus_tag="KAM330_50270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_094965909.1"
                     /translation="MNIPTHLKHKPVLEVINYDQIDGPYANDSDAMGLSIGIAQWNSPG
                     WTDLSAKVWRHTGEKWSRQSEELPLHRVIDLTTLICIAMEYCRNGRLSSSDDFPVSRST
                     DNADLGHHVELMKKELKSGSKYLDESLKRLSLELKRLGY"
                     /name="DUF6530 family protein"
     gene            856..1842
                     /locus_tag="IFA22_RS25580"
                     /old_locus_tag="KAM330_50280"
     CDS             856..1842
                     /locus_tag="IFA22_RS25580"
                     /old_locus_tag="KAM330_50280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_094965910.1"
                     /translation="MTEKAFLRNLPHRPYCTDDKSSGLLIRPQATALGYRHIQYNPPPH
                     VASLVFDLDKPDSYHAWQDAGLPAPHWISVNRTNGHAHIGYMLATPVARTSAARQKPLR
                     YLAAIEHVLAKRLGADMGYAGLITKNPTHSDWLTIWHQIEPYSLDYLAEFCPDADLAAY
                     SRRSRKEATGLGRNVTVFDNVREWAYTAIRAHWRPNGYDAWLCAVQAACECTNVFGLEQ
                     GGPLPHSEIKATAKSIARWTWRNLTPSTFADYVDRTHTPEIQAVRGAKGGRIGGKVSKG
                     GGRPSKAASLLPVVLQLKAQGYSNRDIAEDLQISAGSVSNYLRRDRE"
                     /name="replication initiation protein"
     gene            complement(1995..2285)
                     /locus_tag="IFA22_RS25585"
                     /old_locus_tag="KAM330_50290"
     CDS             complement(1995..2285)
                     /locus_tag="IFA22_RS25585"
                     /old_locus_tag="KAM330_50290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094965911.1"
                     /translation="MKDNADKGTGDMFGIKRGRGRPKTGSAKTGAERQAAYRTKQQENN
                     VTVTINRGLLEGLDAQMQAIRDGGSAVVLTSEQAGEILRALRTAEKKQLRG"
                     /name="hypothetical protein"
     gene            2484..2822
                     /locus_tag="IFA22_RS25590"
                     /old_locus_tag="KAM330_50300"
     CDS             2484..2822
                     /locus_tag="IFA22_RS25590"
                     /old_locus_tag="KAM330_50300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019820367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_094965905.1"
                     /translation="MPTEQPKRLKTIKVRVTETEWASLRAACPKAQLAEWMREYCLGVV
                     PQQRRTPPPTIDPALLRQVAGIGNNLNQIARRINASPLTTLDKVEYLAALAAVARELEG
                     LRRDRQNP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3022..>3456
                     /locus_tag="IFA22_RS25830"
                     /pseudo=""
     CDS             3022..>3456
                     /locus_tag="IFA22_RS25830"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3509..3925
                     /locus_tag="IFA22_RS25835"
                     /old_locus_tag="KAM330_50310"
     CDS             3509..3925
                     /locus_tag="IFA22_RS25835"
                     /old_locus_tag="KAM330_50310"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_232105324.1"
                     /translation="MAKTFEQTSKQQADDIESSLKPEFERHERAIKEALDSSRQSINDA
                     IQDQQKLIGWLLLKSWGVMLISGLLLLAATSGTLWYQGNLIAERQATLEALGKKGGKLI
                     FNKCEDENGKIRLCIRMNMDEGKFGDDYMIPKGY"
                     /name="MbeB family mobilization protein"
     gene            3929..4162
                     /locus_tag="IFA22_RS25840"
                     /old_locus_tag="KAM330_50320"
     CDS             3929..4162
                     /locus_tag="IFA22_RS25840"
                     /old_locus_tag="KAM330_50320"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD/MobD family mobilization/exclusion protein"
                     /protein_id="WP_153913308.1"
                     /translation="MTELERHLLNALETLQSEQDRQLSGLQTACESLLRQFETTKKENA
                     SLLGEVKVLRQQCEHLNEQVSSLATQVASLKA"
                     /name="MbeD/MobD family mobilization/exclusion protein"
     gene            4498..4737
                     /locus_tag="IFA22_RS25600"
                     /old_locus_tag="KAM330_50340"
     CDS             4498..4737
                     /locus_tag="IFA22_RS25600"
                     /old_locus_tag="KAM330_50340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="WP_094965907.1"
                     /translation="MVAAAMRSKRTVSVTLEPSLLERARAAGINLSATLAAALQHEISG
                     SEAERWKRENSAAIQELNRITEEHGLLSDDYRAF"
                     /name="type II toxin-antitoxin system CcdA family
                     antitoxin"
     Typing          3344..4371
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.535"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aattatcgtt gagggggata agtatctggt gatgacgcag gacatggcag cggtgaggca
       61 gacgcagctt ggggaagaag tcacaggagt gcaggagtct cgtcagctca tcaaggacgc
      121 aatagatttc ctgatggatg gtgtttgagg ctttaaaggg aggcagatat ttcggcggtt
      181 ccggcgtaca accccataga cattaccaac taaactcagt aatgtttgtg aaattagtaa
      241 acttgataat ctggggctcg ctatgaacat tccaactcat ttgaagcaca agccggtact
      301 ggaagttatt aactatgacc agattgatgg tccatatgct aatgactctg atgcaatggg
      361 actgtcaatt ggcatcgcac aatggaactc acctggatgg actgacctct cagccaaagt
      421 atggcgtcat acaggcgaga aatggtcaag acagtcagaa gaactaccac tgcatcgtgt
      481 tatcgactta acgacattga tatgtatcgc gatggaatac tgtcgcaatg gtcgcttgtc
      541 atctagcgac gatttcccgg ttagcaggtc tactgataac gctgatttgg ggcatcatgt
      601 tgagctaatg aaaaaagagc tcaagagcgg gagtaaatat ctcgatgaaa gtctaaaacg
      661 actgtcttta gagctaaaaa ggctgggtta ctaacggggc tatgcggtaa cttattgctc
      721 ataaaaagac aaacccccgt tatcgctgga atcttggcgg atctgcgaaa aacgggggct
      781 gtgacgaatc acaagagaag tctaccacat ggatcacacc aaacaaccct gcaccgccaa
      841 taagcgcggt gatcgatgac agaaaaagcc tttctcagga atctccccca tcggccctat
      901 tgcactgacg ataaatcgtc agggctgctc attcgcccgc aggctaccgc actgggctat
      961 cgccatatcc agtacaaccc accgccgcat gtggcgtctt tggtgttcga cttggataaa
     1021 ccggacagtt accacgcttg gcaggatgca ggcttgccag ccccgcactg gatcagcgtt
     1081 aaccgcacga acggccacgc ccatattggg tacatgctgg cgaccccagt agcccgtacc
     1141 agcgccgccc ggcaaaagcc gctgcgatac ctagccgcca tagaacacgt tctagcgaag
     1201 cggctggggg ccgacatggg gtatgcgggg ctgatcacca aaaaccccac ccatagcgat
     1261 tggctcacca tctggcacca aatcgagccc tacagcctcg actatctcgc agagttctgc
     1321 ccggatgccg atttggcggc ctacagccgc cgcagtcgca aagaggcgac tgggctaggc
     1381 cgtaacgtca ctgtgttcga caacgtgcgg gaatgggcct acacggcgat tagagcgcat
     1441 tggcggccta atgggtacga tgcatggctc tgtgccgtgc aagctgcctg tgagtgcacc
     1501 aacgtgtttg gcttggagca gggtggcccg ttgccccata gcgagatcaa ggccacggcc
     1561 aagagcatcg cccgttggac gtggcggaat ctaaccccgt ccacgttcgc ggattatgtt
     1621 gatcggacgc atacaccaga gattcaggcc gtcagagggg ctaagggcgg gcgtatcggt
     1681 ggcaaggtat ccaagggcgg aggcaggccg agcaaagctg cttctctcct acctgtggtg
     1741 ctacaactca aggcgcaggg ttacagcaat cgcgatattg cggaggatct gcaaatttcg
     1801 gctggcagtg tgtccaacta cctccgccgt gatcgtgagt gatcaaaaac aaagcctata
     1861 tcagataaca gcgcccgtag ggcgttcctt gagccctaaa agcattcaag agagaggatc
     1921 ttggcgaggg ggggtaacgg cgatcccgta gggctgtcca tcaggccatg tgttcggcct
     1981 gtgggcagta gctgttatcc ccggagctgt tttttctctg ctgttcgtag cgcccgcaag
     2041 atctccccgg cctgttccga ggtcagcacc accgcagaac ccccgtctct gatcgcctgc
     2101 atctgggcat ccagtccttc caatagccct ctatttatcg tcacagtaac gttattctcc
     2161 tgctgttttg tccggtaggc cgcttgtcgt tctgcccctg ttttggctga tccggttttg
     2221 ggtctacccc gtccgcgctt gatgccaaac atgtctccgg ttcccttatc agcgttgtct
     2281 ttcatcgtcc taattcccgt cacagtgacc ataaatatta tctgtcactg tgacacaaaa
     2341 accattaatc cgcaccctca aaatccaacc catgttaaag tactaagcaa ggacacacag
     2401 ggggcccgct tcgcggtcta tcccctgtgg ccttgttcag ggggcaagcc ccccgaaacc
     2461 cccgaaaaaa acggaggcca tcgatgccga cagagcagcc aaaacggctc aaaacgatca
     2521 aggtcagagt cactgaaacc gagtgggcca gcttgcgggc ggcctgcccc aaagcccagt
     2581 tggcggagtg gatgcgcgag tactgcttgg gggttgttcc ccagcagcgg cgcaccccac
     2641 cgccaaccat cgatccggcc ttgctccggc aggtggccgg gatcgggaac aacctcaacc
     2701 agatcgcccg gcgcatcaat gccagcccgc tgaccacgtt ggacaaggtg gaataccttg
     2761 cggcgttagc cgccgtggcc cgagaactgg aggggctacg ccgtgatcgt caaaatccat
     2821 agccggggag tcggtcgggg atctggcccg gtggaatacc tgcttggcaa agacgggaac
     2881 cggagcgggg ccacgctgga tcgcggcgac cccgagatgg tcagggagtt gatagacggc
     2941 agccgttacg cccagcggta caccagcggc gtgctgtcgt tccatgaacc ggacttgccg
     3001 agggagacca aggatcggtt gatggacgag ttcgagcaag ccctgttgcc cggactggac
     3061 gccgatcagt atgcggtgct gtgggtcgag caccgagaca aagaccgcct agagctgaat
     3121 ttcgtggtgc cgaacgtcga actgttgtcc ggcaaacgcc ttcagccgta tttccacggg
     3181 gcggacggcc accggatcaa cgcatggcga acggccatca atggcgactt gggattgcac
     3241 gacccagacg acccaatcaa tcggcaggcc gtgactacgg ccaaagacct accccggaac
     3301 aagctggaag ccgaacaagc cattacaggc ggcttgctgg gcatggctgc ggcggggctg
     3361 gttaaaaacc gggaggacgt gctcaagacc ctgaccgggg cggggttcga cgttaccagg
     3421 acaaccaaga acagtatcag cattgccgac cctgaagggg gccggaatat ccgcctaaag
     3481 ggggcgatct atgagcaaga ttttagacat ggcaaagacc ttcgagcaga catcgaagca
     3541 gcaagccgac gatatagaga gcagtctgaa gccagagttc gagaggcacg agcgagctat
     3601 caaagaagcg ttggactcaa gcaggcagag catcaacgac gccatccaag accagcagaa
     3661 gctgatcggc tggctcctgc tcaagagctg gggagtgatg ttgatctcgg gcttgctgtt
     3721 gctggccgcg acgagcggga cgctgtggta tcaggggaac ctgatcgccg agcggcaggc
     3781 caccttggag gcgttgggca aaaagggcgg gaaattgatc ttcaacaagt gcgaggacga
     3841 gaacgggaag atccggcttt gcatcaggat gaacatggac gaagggaagt tcggggacga
     3901 ttacatgata ccgaagggct actaaacgat gaccgagcta gaacggcact tattgaacgc
     3961 gctagagacc ttgcagagcg agcaagatcg gcaattgagc ggcttgcaaa cagcgtgcga
     4021 gagcttgcta cggcagttcg agacgacgaa aaaagaaaac gcgagcttgc tgggagaagt
     4081 gaaagtcttg cgccagcagt gcgagcactt gaacgagcag gtcagcagct tggcaacgca
     4141 ggtcgccagc ttgaaggcat gatcgcgcag cagacggcgc agacgcagga ggggttattg
     4201 gacgaatacc agcaggccca cgcagaacgg gatctctatt tcaatcggaa gcacgagcag
     4261 aaagagatct ccggcttgcc gatagacaag gccagagagt acctacagga aggactggag
     4321 gggcggatag agcggcagca ggtccgcgaa cgggggcgtg gctttgagat gtagctgccg
     4381 cgctgcgctt ggcggcccct gccccgagcg cgacagactt cgctagtcgc acccgtcgca
     4441 agggcgtgat attatcatac acgtgctatc gcacgtgtta atttattgga ggtcatcatg
     4501 gtcgcagcag caatgcgcag caagagaacg gtcagcgtca cgctggagcc cagcttgctg
     4561 gaacgagcca gagcggcggg gatcaacctg tccgccaccc ttgcagccgc actacagcat
     4621 gaaatcagcg gatcagaagc agaacgctgg aagcgtgaga atagtgcagc catacaggag
     4681 cttaaccgca tcaccgagga gcacggtttg ctttccgacg attacagggc gttctgatgc
     4741 agttcacgat ctaccgcaac aagggcaatg cccgcgctta cccctatctg ctggatgtgc
     4801 aaagcgacat catcggcgag ctggccacca gaatagtgat cccactgttc ccgctggcgg
     4861 ccttcaccgg gcggccagcg caacggctga atccggt
//
