LOCUS       NZ_AP023405             5879 bp    DNA     circular CON 08-FEB-2024
DEFINITION  Aeromonas hydrophila strain KAM330 plasmid pKAM330_7, complete
            sequence.
ACCESSION   NZ_AP023405
VERSION     NZ_AP023405.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00242552
            Assembly: GCF_014679585.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas hydrophila
  ORGANISM  Aeromonas hydrophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Suzuki,M., Maehana,S. and Kitasato,H.
  TITLE     Whole genome sequencing of carbapenem-resistant Aeromonas spp.
            isolated in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Suzuki,M., Maehana,S. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2020) Contact:Masato Suzuki National Institute of
            Infectious Diseases, AMR Research Center; 4-2-1 Aobacho,
            Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.4.8.0; Canu v. 1.8
            Genome Coverage                   :: 537x
            Sequencing Technology             :: Illumina MiniSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/08/2024 03:17:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,147
            CDSs (total)                      :: 4,982
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 165
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 126
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 44 of 114
            Pseudo Genes (incomplete)         :: 73 of 114
            Pseudo Genes (internal stop)      :: 12 of 114
            Pseudo Genes (multiple problems)  :: 12 of 114
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023405.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Aeromonas hydrophila"
                     /mol_type="genomic DNA"
                     /strain="KAM330"
                     /db_xref="taxon:644"
                     /plasmid="pKAM330_7"
                     /geo_loc_name="Japan:Kanagawa"
                     /collection_date="2018"
     gene            1..861
                     /locus_tag="IFA22_RS25545"
                     /old_locus_tag="KAM330_50200"
     CDS             1..861
                     /locus_tag="IFA22_RS25545"
                     /old_locus_tag="KAM330_50200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014003865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_190284537.1"
                     /translation="MAINIMEAFQQEPPQLDFIWPGFLAGTVGALVAPGATGKSFWALE
                     AAMSIACSVAGGDVVNLKPQHTGRVVYLAGEDPEPALIRRVHAIGQHLNQSAREAIAEN
                     LTLEPIMGKRLNVMDEAHLRRVIEYSAGARLIVLDTLSRIHSLDENSNGDMARLVATLE
                     HVAASTGASVLYLHHVSKGSAREGQTDQQQAARGASALIDNARWCGFVAKMTEDEAKRL
                     SDRAYDRQPIGNDRRGFFVRFGVSKQNYDATPLDQWYERRDGGVLVPVELVEVPNGKRG
                     SRDEL"
                     /name="helicase RepA family protein"
     gene            851..1744
                     /gene="repC"
                     /locus_tag="IFA22_RS25550"
                     /old_locus_tag="KAM330_50210"
     CDS             851..1744
                     /gene="repC"
                     /locus_tag="IFA22_RS25550"
                     /old_locus_tag="KAM330_50210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_190284538.1"
                     /translation="MNFELTHVRHDPAHCLAPGLFRSLKRGERKRLKLDVTYTHGDDSI
                     RFWGPEPLGVDDLRVLQGLVAMAAVSGEGGRGIVLSPEPKTGEGQQLRLWLDLKWDAIE
                     KDSMVAKGSFRQLARELGYADDGGSQFKTIRESIERLWAVSVIVERGGRRQGFRILSDY
                     ASDEHEGKLFVALNPRLAEAVMGERPHTRIDMGEVRALQTDPARLMHQRLCGWIDPGKS
                     GRVELDTLCGYAWPEQANPEAMKKRRQTARKALAELAAVGWTVSEYAKGKWEVKRPAAP
                     TVTLPNPRRNSPQPPA"
                     /name="repC"
     gene            complement(2020..2487)
                     /locus_tag="IFA22_RS25555"
                     /old_locus_tag="KAM330_50220"
     CDS             complement(2020..2487)
                     /locus_tag="IFA22_RS25555"
                     /old_locus_tag="KAM330_50220"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_190284539.1"
                     /translation="MQFKRQGRRVQVLAYRGYDKEKRRAIVKMMGSIDVYSYEPSDGLI
                     ENLTDEEKTELQSYIETERQAAEKRSRQYSAKSAASRIKEVADTIQGGDFEPSEAWAAD
                     TWAAIEALTKVMRKAGYPKPRKAPQKAADAPMPGQAGLPLGDAPEAPGNAS"
                     /name="hypothetical protein"
     gene            2953..3384
                     /locus_tag="IFA22_RS25560"
                     /old_locus_tag="KAM330_50230"
     CDS             2953..3384
                     /locus_tag="IFA22_RS25560"
                     /old_locus_tag="KAM330_50230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_013250886.1"
                     /translation="MNTDESKRRRRGPAPLDATDKRGHTVSVRLNEAELARLDSQRDAV
                     QMQRGEYLRAAALHRLPPTIPAANREQWAELARTAANLNQIARHLNEGQRGDGERIGKA
                     LGLQLSAELANCLRLLVLVRGDLIGVKHDSEDVEDESEG"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            complement(3575..3808)
                     /locus_tag="IFA22_RS25815"
                     /old_locus_tag="KAM330_50240"
     CDS             complement(3575..3808)
                     /locus_tag="IFA22_RS25815"
                     /old_locus_tag="KAM330_50240"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228283734.1"
                     /translation="MLKLLGRLDGAAGFPLAQPNFPVQPYPACHDVDVILVRVLVAHCH
                     PRRIGLVKAHALHEVSRHGFPLLGAQALTGRQ"
                     /name="hypothetical protein"
     gene            4140..4823
                     /locus_tag="IFA22_RS25820"
                     /old_locus_tag="KAM330_50250"
     CDS             4140..4823
                     /locus_tag="IFA22_RS25820"
                     /old_locus_tag="KAM330_50250"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223928366.1"
                     /translation="MTKLETVRALNDSGPQLQIVESVRTLQQSLSLMHEDLKRLPVAVS
                     TETAQTLEPLARLQQDVTQVLEAYDKVTAIQRKTLDELTEQMSVSAAKAFERKAGELDK
                     TISDLSNSLSGLETSISSMKETAEQVVALPGNLVSAQQNMAQAAIKLTEAANKTRPRLW
                     RQALGLILAGAVGAMLVATGQVALNRLVPPSDVQQTADWANAIWNKATPKERELLEQIV
                     NRPAN"
                     /name="hypothetical protein"
     gene            4859..5809
                     /locus_tag="IFA22_RS25825"
                     /old_locus_tag="KAM330_50260"
     CDS             4859..5809
                     /locus_tag="IFA22_RS25825"
                     /old_locus_tag="KAM330_50260"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064483931.1"
                     /translation="MKREGRHLSVKAGGDEAYRVTRQQDGRWLWCDRYGNDGGDNIDLV
                     REIEPGTGYAEAVYRLSGAPTVRQQPRPSEPKRQPPQLPAQGLAAREHGRDYLKGRGIS
                     PDTIEHAEKAGMVRYADGGVLFVGYDHTGTAQNATRRAIAPADPVQKRDLRGSDKIYPP
                     ILPGDPAKVWIVEGGPDALALHDIAKRSGQQPPTVIVSGGANVRSFLERDDVQAILKRA
                     ERVTVAGENEKNPEAQAKADAGHQKQAQRVAEITGREVRQWTPKPEQGKDLADINARQV
                     AEIERKRQAEIEAERARNRELSRKSRGYDGPSFGR"
                     /name="hypothetical protein"
     Typing          2..843
                     /name="IncQ2"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.986"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          3369..5806
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.754"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctatca acattatgga ggcgttccag caggaaccgc cacaactgga cttcatctgg
       61 ccgggctttc tggctggcac cgtgggcgca ctggtggccc ccggcgcgac cggcaagagc
      121 ttctgggcgc tggaagcggc tatgtccatc gcttgcagcg tggcaggcgg tgacgtggtg
      181 aacctcaagc cgcagcacac cgggcgcgtg gtctatctgg ccggtgaaga tcccgaaccc
      241 gccctgatcc ggcgcgttca cgccatcggc cagcacctca accagtcagc ccgcgaagcc
      301 atcgccgaga acctgaccct tgagccgatc atgggcaagc gcctgaacgt catggacgag
      361 gcgcacttgc gccgcgtcat cgagtacagc gccggggcgc ggctgatcgt gctggacacc
      421 ctgagccgca ttcacagcct cgacgagaac agcaacggcg acatggcgcg gcttgtcgcc
      481 acgctggaac acgtcgcggc cagcactggc gcgtctgtcc tgtacctgca ccacgtcagc
      541 aagggcagcg cccgcgaggg acaaaccgac cagcagcagg cagcgcgggg cgcatcggcc
      601 ctgatcgaca acgcccgctg gtgcggcttc gtcgccaaga tgaccgagga cgaggccaag
      661 cgcctgagcg accgcgccta tgaccgtcag cccatcggca acgaccggcg cggcttcttc
      721 gtgcgcttcg gcgtgagcaa acagaactac gacgccaccc cgcttgacca gtggtatgag
      781 cgccgagacg gcggcgtgct ggtgccggtg gaactggtgg aagtgccgaa cggcaagagg
      841 gggagccgcg atgaactttg aacttaccca cgtccggcac gatccggcgc attgtctggc
      901 ccccggcctg ttccgcagcc tcaagcgagg cgagcgcaag cggctcaagc tggacgtgac
      961 ctacacccac ggcgacgact caatccgttt ctgggggcct gagccgctgg gcgttgatga
     1021 cctccgcgtg ttgcaaggat tggtggcaat ggccgctgta tcaggcgaag gcggacgcgg
     1081 cattgtgcta tcgcctgaac ccaaaaccgg ggaagggcag caattacgcc tatggctcga
     1141 tctgaaatgg gatgccatcg agaaagactc gatggtagcc aagggcagct ttcgccagtt
     1201 ggcccgagaa ctgggttatg ccgacgacgg cggcagccag tttaagacca tccgggaaag
     1261 catcgagcgt ctatgggctg tatcagtcat cgtggagcgc ggcgggcggc ggcaaggctt
     1321 ccgcatcctg tcggactacg cgagcgacga gcatgaaggc aagctgttcg tcgccctgaa
     1381 tccacggctg gccgaggccg tcatgggcga gcgcccccac actcgcatcg acatgggcga
     1441 agtgcgggcg ctacagaccg acccggcgcg gctgatgcat cagcgcctat gcggctggat
     1501 cgaccccggc aagtccgggc gggtagagct ggacacgctt tgcggctacg catggcccga
     1561 gcaagccaac ccggaagcca tgaagaagcg gcgccagacg gcccgcaagg cactggccga
     1621 gcttgccgca gtgggctgga cggtgagcga gtacgccaag ggcaaatggg aggtcaagcg
     1681 gcccgccgcc cccacggtaa ctctccccaa cccccggcgt aactctcccc aacccccggc
     1741 gtaactctcc ccaacccccg gcgtaactct ccccgccccg atctggcaaa cccagacggg
     1801 gcgcggcttt gcgggcgatt cgcaaaatcc atccaagatt ctccaagaat ctccactagg
     1861 cgcagcacag cccgcgcccc ttcggcgcgg tctggcattg cctcaaatgc ccggccaagg
     1921 ccggaaggct gaacacctct ccccccgcct tcggcgaccc cctccggggg taaagggcca
     1981 gcggggagag gggtggggcc ggtttcccgg cctaacccct caggaagcgt ttcccggcgc
     2041 ttctggcgca tcccccaagg gcaaccctgc ctgacccggc atcggcgcgt ctgcggcctt
     2101 ctggggcgct ttgcggggct tcggatagcc cgccttgcgc atgaccttgg tcagtgcctc
     2161 gattgccgcc caggtatccg ccgcccacgc ctcggacggc tcgaaatcgc ccccttggat
     2221 cgtgtcggca acctctttaa tccgactggc ggcagactta gcggaatact ggcggcttcg
     2281 tttttctgcc gcctgtcgct ctgtttcaat gtatgactgt agttctgttt tttcttcgtc
     2341 tgtcagattc tcaattagac cgtctgacgg ctcgtaagaa tatacgtcaa tagaccccat
     2401 catcttgacg atggcgcggc gcttttcctt gtcatacccc cggtaggcga gcacctgaac
     2461 ccggcgacct tggcgcttaa actgcatggc ggtttctccg tttgactgtg tgattgtatt
     2521 ttagtctgac agtcgcacgc tgtcaagtgt ttattgtcgg tttcagtttt aatacgactg
     2581 tcgcacttaa ataagtttgc actgtcgtat tgaattccgc ttgacagtct gccgcgcctc
     2641 cgataccata gcggtatggt gtcagttcgc cccgccgctt tgcccacccc ccacccccct
     2701 ctccccgctg gcccattacc gcccttgggc ggtcgggccg agagcgcagc gagcgggccg
     2761 ggtgggtggc tgttcggtgc ttgcatggag atgtgctatt atggtattga ggctgtggtt
     2821 tggcatggag caaggtgtaa cctgcgcttc gcttggttct caccctccgg ccttcggcct
     2881 acgcccccac tcccttcggt cggggggttg ctgcgcgttt cgcttcgctt cacttactgg
     2941 agggcttcgc ccatgaacac tgacgaatcc aagcgccgcc gccgtgggcc tgcgccgctc
     3001 gacgccacgg acaagcgcgg gcataccgtt tctgtgcgcc tcaacgaggc cgagcttgcg
     3061 cggctggatt cgcagcgtga cgcggtgcag atgcagcggg gcgagtacct acgcgccgcc
     3121 gctctgcatc gcctgccgcc caccatcccg gcagcgaacc gggaacagtg ggcagagttg
     3181 gcccgcacag cggccaacct gaaccagatc gcccggcacc tgaacgaagg gcagcggggc
     3241 gacggggagc gcatcggcaa ggcgctgggc ttgcagttga gcgccgagct tgccaactgc
     3301 ctgcgattgc tggtgctggt gcgcggcgac ttgataggcg tcaagcacga ctcggaggac
     3361 gtggaagatg aaagcgaagg ttagccgtgg tggcggtttt cgcggtgcgc tgaactacgt
     3421 ttttgacgtt ggcaaggaag ccacgcacac gaaaaacgcg gaacgtgtcg gcggcaacat
     3481 ggccgggaat gacccccgcg aactgtcgcg ggagttctca gccgtgcgcc agttgcgccc
     3541 ggacatcggc aagcccgtct ggcattgctc gctgtcactg cctcccggtg agcgcctgag
     3601 cgccgagaag tgggaagccg tgacggctga cttcatgcag cgcatgggct ttgaccagac
     3661 caatacgccg tgggtggcag tgcgccacca ggacacggac aaggatcaca tccacatcgt
     3721 ggcaagccgg gtagggctgg acgggaaagt ttggctgggc cagtgggaag cccgccgcgc
     3781 catcgaggcg acccaagagc ttgagcatac ccacggcctg accctgacgc cggggctggg
     3841 ggatgcgcgg gccgagcgcc ggaagctgac cgacaaggag atcaacatgg ccgtgagaac
     3901 gggcgacgaa ccgccgcgcc agcgtctgca acggctgctg gatgaggcag tgaaggacaa
     3961 gccgaccgcg ccagaactgg ccgaccggct atatgccgca ggcgtaggcg tccgggcaaa
     4021 cctcgccagc accgggcgca tgaacggctt ttccttcgag gtggccggag tgccgttcaa
     4081 gggcagcgac ttgggcaagg gctacacatg ggcggggcta cagaaatcag gggtgactta
     4141 tgacgaagct agagaccgtg cgggccttga acgattcagg cccacagttg cagatcgtgg
     4201 agagcgtcag gacgttgcag cagtccctga gcctgatgca cgaggacttg aagcgcctac
     4261 cggtcgcagt ctcgaccgag acggcgcaga ccttggaacc gctggcccga ctccagcagg
     4321 acgtgacgca ggttctggaa gcctacgaca aggtgacggc cattcagcgc aagacgctgg
     4381 acgagctgac ggagcagatg agcgtgagcg cggcgaaggc gttcgagcgg aaggccgggg
     4441 agctggacaa gaccatctcc gacctgtcca acagcctgtc agggctggaa acgagcatca
     4501 gcagcatgaa ggagaccgcg gagcaggtgg tggccttgcc gggcaatctg gtgagcgcac
     4561 agcagaatat ggcgcaagcc gccatcaaac tgaccgaggc agcgaacaag acgcgcccgc
     4621 gcctttggcg gcaggcgctg gggctgattc tggccggggc cgtgggcgcg atgctggtag
     4681 cgactgggca agtcgcttta aacaggctag tgccgccaag cgacgtgcag cagacggcgg
     4741 attgggccaa cgcgatttgg aacaaggcca cgcccaagga gcgcgagttg ctggaacaga
     4801 tcgtcaatcg gcccgcgaac tagatccgac cgcctacctt gaggcgagcg gctacaccgt
     4861 gaagcgcgaa gggcgacacc tgtccgtcaa ggctggcggt gatgaggcgt accgcgtgac
     4921 ccggcagcag gacgggcgct ggctctggtg cgaccgctac ggcaacgacg gcggggacaa
     4981 tatcgacctt gtgcgcgaga tcgaacccgg caccggctac gccgaggccg tctatcggct
     5041 ttcaggtgcg ccgacagtcc ggcagcaacc gcgcccgagc gagccgaagc gccaaccgcc
     5101 gcagctaccg gcgcaagggc ttgcagcccg cgagcatggc cgcgactacc tcaagggccg
     5161 gggcatcagc ccggacacca tcgagcacgc cgagaaggcg ggcatggtgc gctatgcaga
     5221 cggtggcgtg ctgttcgtcg gctacgacca tacaggcacc gcgcagaacg ccacacgccg
     5281 cgccattgcc cccgctgatc cggtgcagaa gcgcgaccta cgcggcagcg acaagatcta
     5341 tccgccgatc ctgccgggcg acccggcaaa ggtctggatc gtggaaggtg gcccggatgc
     5401 gctagccctg cacgacatcg ccaagcgcag cggccagcag ccgcccaccg tcatcgtgtc
     5461 aggcggcgcg aacgtgcgca gcttcttgga gcgtgacgac gtgcaagcga tcctgaagcg
     5521 ggccgagcgc gtcaccgtgg ccggggaaaa cgaaaagaac cccgaggcgc aggccaaggc
     5581 cgacgccggg caccagaaac aggcgcagcg ggtggccgaa atcaccgggc gcgaggtgcg
     5641 ccaatggacg cccaagcctg agcagggcaa ggacttggcc gacataaacg cccgtcaggt
     5701 ggccgagatc gagcgcaagc gacaggcaga gatcgaggcc gagagggcac gaaaccgcga
     5761 gctttcacgg aagagccggg ggtatgatgg ccccagcttc ggcagataag agcgcctgtc
     5821 agtcggtgtg atgaaccgac cgacaggcta accatcatca acctgagtag gaggccgat
//
