LOCUS       NZ_AP023404             8117 bp    DNA     circular CON 08-FEB-2024
DEFINITION  Aeromonas hydrophila strain KAM330 plasmid pKAM330_6, complete
            sequence.
ACCESSION   NZ_AP023404
VERSION     NZ_AP023404.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00242552
            Assembly: GCF_014679585.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas hydrophila
  ORGANISM  Aeromonas hydrophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Suzuki,M., Maehana,S. and Kitasato,H.
  TITLE     Whole genome sequencing of carbapenem-resistant Aeromonas spp.
            isolated in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8117)
  AUTHORS   Suzuki,M., Maehana,S. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2020) Contact:Masato Suzuki National Institute of
            Infectious Diseases, AMR Research Center; 4-2-1 Aobacho,
            Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.4.8.0; Canu v. 1.8
            Genome Coverage                   :: 537x
            Sequencing Technology             :: Illumina MiniSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/08/2024 03:17:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,147
            CDSs (total)                      :: 4,982
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 165
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 126
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 44 of 114
            Pseudo Genes (incomplete)         :: 73 of 114
            Pseudo Genes (internal stop)      :: 12 of 114
            Pseudo Genes (multiple problems)  :: 12 of 114
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023404.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8117
                     /organism="Aeromonas hydrophila"
                     /mol_type="genomic DNA"
                     /strain="KAM330"
                     /db_xref="taxon:644"
                     /plasmid="pKAM330_6"
                     /geo_loc_name="Japan:Kanagawa"
                     /collection_date="2018"
     gene            complement(join(7822..8117,1..250))
                     /locus_tag="IFA22_RS25480"
     CDS             complement(join(7822..8117,1..250))
                     /locus_tag="IFA22_RS25480"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_104453315.1"
                     /translation="MEEMKKRGRPSTGIAKTSAERSADYRARKAAELAEREAKWRELEA
                     EREALDKAWCAERGMDWNEFQEVRREASRNAAQYARDLMNKLLEREADEEREQEAFCVR
                     EGIDWREVKKYLNRQKGDIKKLTKEIEAMSADYHRMEALKKREAVVMDREAVLAKREAL
                     VAERESALAIREQLPVTK"
                     /name="hypothetical protein"
     gene            complement(653..829)
                     /locus_tag="IFA22_RS25485"
                     /old_locus_tag="KAM330_50060"
     CDS             complement(653..829)
                     /locus_tag="IFA22_RS25485"
                     /old_locus_tag="KAM330_50060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_190284536.1"
                     /translation="MLWHTVAVLAAVFAAKALLYWAADWLVLGPLYSVLAGPSWDVAAW
                     IAALALLLRWMRV"
                     /name="hypothetical protein"
     gene            complement(875..1081)
                     /locus_tag="IFA22_RS25490"
                     /old_locus_tag="KAM330_50070"
     CDS             complement(875..1081)
                     /locus_tag="IFA22_RS25490"
                     /old_locus_tag="KAM330_50070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017780732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042650714.1"
                     /translation="MEFDKHDLEIDGDRVWLLDADGQRLCDLNDMRLLDFEWRISVEGG
                     LLNFDLEASEWRQRLLDAGLQLD"
                     /name="hypothetical protein"
     gene            complement(1183..1470)
                     /locus_tag="IFA22_RS25800"
                     /old_locus_tag="KAM330_50080"
     CDS             complement(1183..1470)
                     /locus_tag="IFA22_RS25800"
                     /old_locus_tag="KAM330_50080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_232105323.1"
                     /translation="MIDRLERMEAAHQRQTAALEQRLQQQALSLNELQTACTSALVSCG
                     TLCSELQRSFEALQSGVERSNRATTTAFGSLNNSVNELSKALDALHKAQS"
                     /name="mobilization protein"
     gene            complement(1492..2025)
                     /locus_tag="IFA22_RS25805"
                     /old_locus_tag="KAM330_50090"
     CDS             complement(1492..2025)
                     /locus_tag="IFA22_RS25805"
                     /old_locus_tag="KAM330_50090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042650716.1"
                     /translation="MEHLGPQKAQLSERLKLQHEQALGEVEKLAKAVLDELKKDLKKRV
                     NDELNTTVGAIRDGIEGLPQQITDSADPLREVLEQARSEANTLKLISSVWLKALLVGVC
                     TSVGIGLGSWGVLSWMGSEVQRLNSEISSAKATLAELPTGVTFVHQGGKSYILAPSMGE
                     PFETKSGKMAVEVN"
                     /name="hypothetical protein"
     gene            complement(<2075..2734)
                     /locus_tag="IFA22_RS25810"
                     /pseudo=""
     CDS             complement(<2075..2734)
                     /locus_tag="IFA22_RS25810"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2724..3137)
                     /gene="mobC"
                     /locus_tag="IFA22_RS25500"
                     /old_locus_tag="KAM330_50110"
     CDS             complement(2724..3137)
                     /gene="mobC"
                     /locus_tag="IFA22_RS25500"
                     /old_locus_tag="KAM330_50110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_042650709.1"
                     /translation="MSRVPKTTTISLRIDKPTAAEWRERAAASGLSVSDWIRGAVDAGQ
                     QTNLPTPQRRPVRVRDTSNDADPALMRQLAALGSNLNQIARSLNECRLVGAPVRLVEVL
                     ALLRSIEAEAAHLLPQLPPPPKPTTKESSSDAR"
                     /name="mobC"
     gene            3345..3635
                     /locus_tag="IFA22_RS25505"
                     /old_locus_tag="KAM330_50120"
     CDS             3345..3635
                     /locus_tag="IFA22_RS25505"
                     /old_locus_tag="KAM330_50120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042650710.1"
                     /translation="MKDTKDQGTGDMFGIKRGRGRPKTGSAKTGAERQAAYRAKQQDNN
                     VTVTINRALVEGLDAQMQAIRDGGSAVVLTPEQAGEILRALRTAENKQLRG"
                     /name="hypothetical protein"
     gene            complement(3790..4704)
                     /locus_tag="IFA22_RS25510"
                     /old_locus_tag="KAM330_50130"
     CDS             complement(3790..4704)
                     /locus_tag="IFA22_RS25510"
                     /old_locus_tag="KAM330_50130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_223936815.1"
                     /translation="MIRPKATALAYRHIQHNPPPHVACLVFDIDRPDGYAAWESAGLPA
                     PHWIAINLDEGLDENGVCHRGRAHIGYMLATPVARTSAAKAKPLRYLAAIEHVMAHRLG
                     ADMRFTGLITKNPTHSDWWTVWHPIEPYSLDYLAEFCPDKDLAAYSRRSRKEASGLGRN
                     VTLFDNVREWAYSAVREYWRPNGYDAWAEAVRAACDSANAFGLEQGGPLPVSETKATAK
                     SIARWVWRHFTPSEFRAIQATRGAKGGKVSKGGGRPMGTSRTNWELWEAIQSMKAAGYP
                     HSTIADDLNISASTVSKYAKISK"
                     /name="replication initiation protein"
     gene            complement(4971..5258)
                     /locus_tag="IFA22_RS25515"
                     /old_locus_tag="KAM330_50140"
     CDS             complement(4971..5258)
                     /locus_tag="IFA22_RS25515"
                     /old_locus_tag="KAM330_50140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321942.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_068979439.1"
                     /translation="MTFELEFDPRAWKEWKKLGDTVRQQFKKKLSEVIERPRIEANRLN
                     GMSDCYKIKLRGAGYRLVYQVQDDRVVVFVVAVGRREREEVYLDAMSRLD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5248..5490)
                     /locus_tag="IFA22_RS25520"
                     /old_locus_tag="KAM330_50150"
     CDS             complement(5248..5490)
                     /locus_tag="IFA22_RS25520"
                     /old_locus_tag="KAM330_50150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_068979438.1"
                     /translation="MGTINLRIDDELKARSFAVLDKLGVTPSDIIRQTLEYVASQGRSP
                     FRTTVVTEDEEALLAIVRERLASPQKGIKVSLDDL"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            5669..6298
                     /gene="parA"
                     /locus_tag="IFA22_RS25525"
                     /old_locus_tag="KAM330_50160"
     CDS             5669..6298
                     /gene="parA"
                     /locus_tag="IFA22_RS25525"
                     /old_locus_tag="KAM330_50160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017158739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="WP_068979437.1"
                     /translation="MIIGLLNQKGGVGKTTLSVNLAASLARRGFRVLLIDADPQGSALD
                     WAAARDGEPLFSVVGLPRATVHKEIGQLGQGYDHIVIDGPPRVTDLARSAIMAADVVLI
                     PVQPSPYDIWAADEVVKLIEEARIYKEGLKAAFVVNRKIANTAIGRDVGAALAAYPIPV
                     LTASITQRVVFAEAAAQGRAVHETDETGPAAAEIDAVMAELMEIAR"
                     /name="parA"
     gene            6295..6528
                     /locus_tag="IFA22_RS25530"
                     /old_locus_tag="KAM330_50170"
     CDS             6295..6528
                     /locus_tag="IFA22_RS25530"
                     /old_locus_tag="KAM330_50170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006924210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_068979436.1"
                     /translation="MSKKVTIGAKPTSKSAQASADEWVENRSTGNAPEPMKRLTIDIPE
                     SLHRAIKMQCASRGAKIADEVRELLLKKYGNL"
                     /name="hypothetical protein"
     gene            complement(6643..7227)
                     /locus_tag="IFA22_RS25535"
                     /old_locus_tag="KAM330_50180"
     CDS             complement(6643..7227)
                     /locus_tag="IFA22_RS25535"
                     /old_locus_tag="KAM330_50180"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_104453313.1"
                     /translation="MKKELSNYGMAAKLKLSMDKDDPDDLELTTECIKEQLLKRSHNHS
                     LSDMDYRFYIKILWFGFIDEGYGFGSIVAFDRTTRSDQKDKYVELFHFERNEIILYDKS
                     IVRPRLRRLTHQEKTRLPEVISVFIQVNALLEIERLAKEYSWSENELSSDEFKAREEVR
                     KEIFDEASDYSESCFRSENEGWFYSDTDDEY"
                     /name="hypothetical protein"
     gene            complement(7508..7765)
                     /locus_tag="IFA22_RS25540"
                     /old_locus_tag="KAM330_50190"
     CDS             complement(7508..7765)
                     /locus_tag="IFA22_RS25540"
                     /old_locus_tag="KAM330_50190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="WP_104453314.1"
                     /translation="MECTHHFKQRMTQRGISKRMVDLALDYGKLESNDKYVLRQKDAVK
                     LLSELQDSLRHLKKIIDKGGVTVVVNGESLITTYNCDNQN"
                     /name="DUF4258 domain-containing protein"
     Typing          complement(1125..2734)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.52"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttaagtcacg agcgtactgc gctgcgttgc ggctggcctc gcgtctgacc tcttgaaact
       61 cattccaatc catacctcgt tcagcacacc acgccttatc aagagcttct cgctctgcct
      121 ccaattcacg ccatttagct tcacgctcgg ccaattcggc tgccttgcga gcacgatagt
      181 cggcggagcg ctctgcggag gttttggcga tccctgtact ggggcggccg cgctttttca
      241 tctcttccat tccatgctct cttttgtact gcgtgtcatc ttgattcaaa gaataatcgc
      301 tccgtcacta taatgcaatt agtgacgaca caataaagta gataattttt aaccgcaaaa
      361 gatcccgttt gaggagtaaa cgaggtgcgc ctaacgcctt ttatgttcaa tagccgattt
      421 cgcataccaa aacggcaact ccagtcaaaa ccagcctcct gtatcgcgtt ttggttcatt
      481 gcaacaccca aggcagtgca tttgctgcct gaaagtttgc aacgcttctg aggcggtttt
      541 agggccggat cctgcagggt gtttctggat ctggccacac tcccgaacgg ggacgactag
      601 cgcagtctgt cgcgccccct tcgggagtcg ccaagcgcag cgcggcaggc agttagaccc
      661 gcatccagcg cagcagcagg gccagcgccg caatccatgc cgccacatcc cacgaaggcc
      721 cggctaagac gctgtaaagt ggccccagca ccaaccagtc cgcagcccag tacagcaggg
      781 ctttcgccgc gaacacggcg gccagtaccg ccaccgtatg ccacaacatc aaccccggaa
      841 atctatcgct caaccacatc ggcaccacct cccttcaatc caactgaagg cccgcatcga
      901 ggagccgctg ccgccactcg ctggcctcca aatcgaagtt gagcaagccg ccttccacgc
      961 tgatccgcca ttcaaaatcg agcaaccgca tgtcgttcag gtcgcacagc cgctgaccgt
     1021 ctgcgtccaa tagccatacc cgatcaccat caatctcaag atcgtgctta tcgaattcca
     1081 ttgccccgct ccctatattt atcgtcacag tgacaaaata tcacatactc aacccgctac
     1141 tctggctctg ctcttcacgc agcgctttag ccctgttggc gatcagctct gcgccttgtg
     1201 taaggcgtcc agcgccttgc tcaactcgtt gacactgttg ttcaagctgc caaacgctgt
     1261 cgtggtcgct ctgttcgacc gttctacccc gctctgcaac gcctcgaacg aacgctgtag
     1321 ctcgctgcat aacgtcccgc agctcaccag cgcgctggtg caagcggtct gcaactcgtt
     1381 caaagagagc gcctgctgct gcaatcgctg ctccaaagcc gccgtctgcc gttggtgcgc
     1441 cgcttccatc cgctccaacc tgtcgatcaa tatccgctcc agttcggtca ttcagttcac
     1501 ctccacggcc attttgccgc tcttcgtttc gaacggttcc cccatgctgg gggcgaggat
     1561 gtagctcttg cccccctgat ggacgaaggt cactccggtc ggaagctccg ccaacgtggc
     1621 tttcgcgctg ctgatctcgc tgttcaaccg ctgaacctcg ctgcccatcc agctcaacac
     1681 cccccagctc cccagcccga tccccacgga ggtgcatacc ccgaccagca aggctttgag
     1741 ccagacgctg gagatcagct tgagggtgtt ggcctcgctc cgtgcctgct ccaagacctc
     1801 cctcaatggg tcggccgagt cggtgatctg ttggggcaat ccctcgatcc cgtcccggat
     1861 agcgcccaca gttgtattca gctcgtcgtt cacacgcttt tttaagtctt tcttgagctc
     1921 gtcgagcacc gctttcgcta acttctccac ctcgcccaat gcctgctcgt gctgcaactt
     1981 gagcctctcg ctcaattgcg ccttttgcgg tccaagatgc tccatataac tctcccctta
     2041 accggacggc tttggggtag ccctccggct tgatgctgat gtaatccatg cccgttcgcg
     2101 tgatttcgca cccgagcgat tcttgcaggt aggcccgcag ctcggcggcg tttgtgatca
     2161 ccccctgcgc ggtggccccg attaggtggt cggtgatttg ttttttgatg gccgggtggt
     2221 cgcccttgta tgcctcgaaa tccggccgca gcagccgtgc ccggagcggg tcgtctggcc
     2281 gcgcccagcc cttggcatga ttgtgtttat ccctgaccgc atagaagtcg tcgcgttgac
     2341 ctggtggggc cgggttgaac gattttccgc tgcccaggtg aacacggggg atcaggatgt
     2401 gcacatgcgc cccgccatcc ggttcgtcgt gctggacgac ggtcatgtgg tagtcctccg
     2461 gctccagtcc ggcaaatgcc agcgcctcga aatcggccag cacctcgtcg atctcggcag
     2521 tggtcggtgc ctcctcagcg gcccacgaaa tcaccgcgct ggagtaccgc tgctggaacg
     2581 gcagtgagtc ggccaccgcc gcgaacagcg ccggatcccc ccgcagtacc gtcaccccgg
     2641 cccgctccgc tcctgtgtgg tcgtgcgccc caagcaggta ggccgaggcg cgggcgccgc
     2701 tgcccgtacc gtgggctagg aatttaacgt gcatcgctgc tgctctcctt ggtggtcggc
     2761 ttggggggcg gaggcaactg gggcagcagg tgggccgcct ccgcctcgat gctgcgcagc
     2821 agggccagca cctccaccag tcggaccggt gcaccgacta gccggcactc attcaggcta
     2881 cgggcgatct ggttgaggtt cgatcccaac gcggccagct ggcgcatcag cgccggatcg
     2941 gcatcgttgc tggtgtcacg gacacggacg gggcggcgct gtggcgtcgg caagttcgtc
     3001 tgctggccag catccaccgc ccccctgatc cagtccgaca cgctgagccc agaggctgct
     3061 gctcgctccc gccattcggc ggcagtgggc ttgtcgatcc gtaggctgat ggtggtcgtt
     3121 ttcgggactc tggacacggt gcctccggct gttttgtgtg ggggattgaa ggggctaggc
     3181 ccctcaccag ccccgtatta ctggaggccg cgaagcgggc gaaaagtaat acgggtacgc
     3241 tggcttaatg ccagtacagc aaaaaaacag ggggtcggca acttgccaat tatcgtcaca
     3301 gtgacacata atcaattacc gttacagtga caggaaataa caccatgaag gacaccaagg
     3361 atcaggggac tggggacatg tttggcatca agcgcggacg gggtaggcca aaaaccggat
     3421 cagccaaaac aggggcagaa cgacaagcag cctaccgtgc aaagcagcag gacaataacg
     3481 ttactgtgac gataaatcgg gcattggtag aggggctgga tgcccagatg caggctatcc
     3541 gggatggagg gtcggcggtg gtactgaccc cggagcaggc cggggagatt ctgcgggcac
     3601 tgcgaacggc agagaacaaa cagctccggg gataagacct actgcccaca ggccaaacac
     3661 atggcctgat ggacagccct tcgggatcgg ccttaccccc ctcgcaacga gcctctctct
     3721 cttaaatgac tttcgagaag gggccgccct acgggcgctg ttatctgata tggcttgttt
     3781 tcgcgatcgc tattttgaaa ttttggcgta cttgcttacc gtactggcgg aaatattgag
     3841 atcgtcggcg atggtgctat gcgggtatcc ggcagccttc atgctctgaa tggcttccca
     3901 caactcccag ttagtccggc ttgttcccat gggcctcccg ccccccttgg aaaccttgcc
     3961 ccccttggcc cctctggttg cctgaatggc gcggaactcg gacggggtga aatgtcgcca
     4021 tacccaacgg gcgatgctct tggccgtggc cttcgtctcg gacacgggca acggaccgcc
     4081 ctgctccagc ccaaacgcat tggcactgtc acaggcagct ctgacagcct cagcccatgc
     4141 gtcgtaccca ttgggtcgcc aatactctct gacggcgctg taagcccact cccgcacgtt
     4201 gtcgaacagg gtgacgttac gccctagccc gctcgcctcc ttgcggctgc ggcgactgta
     4261 ggcggcgaga tctttatccg ggcagaactc ggccagatag tcgagagaat agggctcaat
     4321 ggggtgccag accgtccacc aatcgctgtg tgtggggttc ttggtgatga ggcctgtgaa
     4381 ccgcatgtca gcccctaacc gatgcgccat aacgtgttct atggcggcca gatagcgcaa
     4441 tggtttcgcc ttggctgcgc tggtgcgggc cacaggggta gccagcatgt aaccgatgtg
     4501 ggcacggcct cgatgacaga ccccgttctc gtccagccct tcatccaggt tgatggcgat
     4561 ccagtgtgga gctggcagtc cagcggactc ccatgcggcg tatccatctg gtcggtcgat
     4621 atcgaatacc aggcaggcga cgtgcggcgg cgggttgtgc tggatgtggc gataggccag
     4681 cgcggtagcc ttcgggcgga tcaacaggcc ctgtgacggg tcatcagtac agtaggggcg
     4741 atgaggcaga cgttcgacaa acgaggcgcg agaggctgtt ttcgtcaatg atgcccgcgc
     4801 ttattggcgg ggcagggttg tttagcgtga tccatgtgtt attattttcc tgttcttagt
     4861 gaacgacccc gaactctctt accgactccg ccaagatgaa gataagagat tcggggtttg
     4921 tcttttctgg ccagtacgtt accgcaggcg gtcacacctt acaacgtccg ctaatcaaga
     4981 cgtgacatgg catccagata tacctcttcc cgttcgcgcc tccccactgc gaccacgaat
     5041 accaccaccc gatcatcctg tacctggtaa accaaccgat accccgcgcc acgcaatttg
     5101 atcttgtagc aatcagacat cccattcagc ctgtttgcct caatcctggg gcgctcgatc
     5161 acctctgaaa gtttcttttt gaactgctgg cgaacggtat cgcccaactt tttccactct
     5221 ttccacgctc gcggatcaaa ctcgagttca aaggtcatcc agcgatacct tgatgccttt
     5281 ctgcgggctg gccaagcgct ctcgcacgat ggctaacagt gcctcttcgt cctccgtcac
     5341 cacggtagtg cgaaacgggc tccgcccctg agaggccaca tactccaggg tttgccggat
     5401 aatatccgag ggggtcaccc ccaatttgtc cagcaccgca aatgaacgtg ccttcagttc
     5461 atcatcaatg cgcaggttaa tcgtgcccat aaccgcctca ctttgtaatt tcaaacgcca
     5521 ttacattgta atactggcaa taccgttgca caaccagaac tagggagtgc aggccggacg
     5581 aactcatgga gtctggaact tattttgatt gctcattgca tgtgcttttc tgtattttta
     5641 caaaaccaca aataatgaga attcaaccat gatcatcgga ctgctcaacc agaagggcgg
     5701 cgttggcaag accaccctca gcgtgaacct ggcggctagc ttggcccgcc gcggctttcg
     5761 ggttcttctg atcgatgccg atccacaagg ctccgccctg gactgggccg cagctcgtga
     5821 tggcgagccc ctgttctcgg tcgtcggcct ccccagagcc accgtgcata aggagatcgg
     5881 acaactcggc cagggctatg accacattgt catcgacgga ccaccacggg taaccgacct
     5941 cgctcgctcc gcgatcatgg ccgctgatgt ggtgctgatc ccggtacaac ccagccccta
     6001 cgacatctgg gcagcagacg aggtggtgaa gctgattgaa gaggctcgca tctacaagga
     6061 aggactgaaa gccgcttttg tggttaatcg taaaatcgca aatacagcca tagggcggga
     6121 tgttggtgca gctctggccg cctaccccat acctgtccta accgccagta tcacccagcg
     6181 cgtagtgttc gcagaggccg cagcccaagg ccgcgctgtt catgaaactg atgagaccgg
     6241 tccagccgcc gcagagatcg atgccgtcat ggcagaattg atggagattg cacgatgagc
     6301 aagaaggtca ccatcggcgc caagccgacc agcaagagtg cgcaggcctc cgccgatgag
     6361 tgggtagaga accgcagcac cggcaatgca cctgagccga tgaagcggct caccatcgac
     6421 atccccgaaa gcctgcaccg cgcaatcaag atgcaatgtg catcgcgcgg agccaagatc
     6481 gcggacgagg tgcgcgaact gcttttgaag aaatacggaa atctgtaatt gcgattttga
     6541 taggcagcaa aaaggggcgg cggatttacc gtaacgcccc ttatgcgcaa ttttttgggg
     6601 gcgacaattc aataataaag ataattccct tcatccatat gtttaatatt catcatctgt
     6661 atcgctgtaa aaccaaccct cgttttcact acggaaacaa ctttcagaat aatcactcgc
     6721 ctcatcaaaa atttcctttc tcacctcctc cctggcctta aattcatcag agcttaattc
     6781 attctcactc caactatatt ccttggcgag gcgttcaatt tctagcaagg catttacctg
     6841 aatgaatact gaaatgactt ctggcaatct tgttttttct tgatgagtta aacgacgaag
     6901 cctaggtcta acgatacttt tatcatacaa aattatttca tttctctcga aatgaaataa
     6961 ttctacatac ttatcttttt gatctgaacg agttgttcta tcgaaggcaa caatactacc
     7021 aaacccatat ccttcatcga taaaaccaaa ccacaaaatt tttatataaa atctataatc
     7081 catatcacta agtgagtgat tatgggagcg cttcagcaat tgctccttaa tacattcagt
     7141 agtcaattcc aaatcatcag gatcatcttt atccatagat aacttaagct tagctgccat
     7201 accataatta ctgagttcct ttttcataaa acctccatga ctacaacgga tgcaacatat
     7261 cacaagcaga acatcaagag aaaaatgaac tcatgccata tgtgacaaga tatgaaaaat
     7321 gcgttctaaa acgatagttt gagttacgct atttgtgttc aagagcctat ggcgaccacc
     7381 aggcggcgcc gatcattacc agttgcacta gagtggaaca gagtgatggt ttacatgggg
     7441 tatgctcaga cacaaggaat agtgcagagc agcattagat ataaggttcc actaaaacat
     7501 agatcaatta attttggtta tcacaattat atgttgtgat taatgactca ccatttacca
     7561 ccacggtcac tccacccttg tcgatgattt ttttaagatg gcgtaatgaa tcctgtaact
     7621 cagacaaaag cttaactgca tccttttgcc gaagaacata tttgtcattg ctttctaatt
     7681 ttccgtagtc tagggccaga tcaaccatgc gcttactgat tccacgttgt gtcattcgct
     7741 gcttgaagtg atgcgtacat tccatgttct aaccctccag taatacactc tcaccatttt
     7801 tgaccaaccc tctagttact gttatttcgt aactggaagt tgctctcgaa ttgccaatgc
     7861 actctcccgt tcggccacta aggcttcgcg tttagccaac accgcttcgc gatccatcac
     7921 tacagcctca cgttttttca acgcttccat acggtgatag tcagcactca ttgcctcaat
     7981 ctccttagtt agtttcttga tgtccccctt ctgcctgttc agatactttt taacttccct
     8041 ccaatcaatg ccttcccgaa cgcaaaacgc ctcctgttct cgttcctcat ccgcttccct
     8101 ttccagaagc ttattca
//
