LOCUS       NZ_AP023403            12311 bp    DNA     circular CON 08-FEB-2024
DEFINITION  Aeromonas hydrophila strain KAM330 plasmid pKAM330_5, complete
            sequence.
ACCESSION   NZ_AP023403
VERSION     NZ_AP023403.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00242552
            Assembly: GCF_014679585.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas hydrophila
  ORGANISM  Aeromonas hydrophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Suzuki,M., Maehana,S. and Kitasato,H.
  TITLE     Whole genome sequencing of carbapenem-resistant Aeromonas spp.
            isolated in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12311)
  AUTHORS   Suzuki,M., Maehana,S. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2020) Contact:Masato Suzuki National Institute of
            Infectious Diseases, AMR Research Center; 4-2-1 Aobacho,
            Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.4.8.0; Canu v. 1.8
            Genome Coverage                   :: 537x
            Sequencing Technology             :: Illumina MiniSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/08/2024 03:17:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,147
            CDSs (total)                      :: 4,982
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 165
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 126
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 44 of 114
            Pseudo Genes (incomplete)         :: 73 of 114
            Pseudo Genes (internal stop)      :: 12 of 114
            Pseudo Genes (multiple problems)  :: 12 of 114
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023403.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12311
                     /organism="Aeromonas hydrophila"
                     /mol_type="genomic DNA"
                     /strain="KAM330"
                     /db_xref="taxon:644"
                     /plasmid="pKAM330_5"
                     /geo_loc_name="Japan:Kanagawa"
                     /collection_date="2018"
     gene            283..1176
                     /locus_tag="IFA22_RS25410"
                     /old_locus_tag="KAM330_49870"
     CDS             283..1176
                     /locus_tag="IFA22_RS25410"
                     /old_locus_tag="KAM330_49870"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139399115.1"
                     /translation="MKKTISLLEATKELMRDVHKKPVITTYDLYCYLLRLYQDKTFDGV
                     KIRGVSLDAPEQRTFDNIISSLQKSGFIHKVEGLDAYYLSGVEKPTTQQILCTVLPFSY
                     ISHLSAMEWHGITDRIPSVVHVTICSNKEFKSLERNKISSELGGASVGKSFSFQRKLSV
                     NDVNGKLINFHEMKCFTPQKEIYGTGGVRVSSIGHTFLSMLQHPEWCGGFSHVYDVFCE
                     NAEKHKAVIIRELDKSGNKIDRSRAGYIMDEVLGIKHPAIDKWKLEVMRGGSRKLSPEN
                     DFSPNYSEVWCLSLNL"
                     /name="hypothetical protein"
     gene            1190..2050
                     /locus_tag="IFA22_RS25415"
                     /old_locus_tag="KAM330_49880"
     CDS             1190..2050
                     /locus_tag="IFA22_RS25415"
                     /old_locus_tag="KAM330_49880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008110254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="WP_139399113.1"
                     /translation="MEVDINAWVNEATPERVSFRQAVHMVLHAVASSEYLKTSMVMKGG
                     MLLGIRYHSSRFTEDIDFSSKMKISDVSTDEFIGSMNDALSLAEDDLGYGIRCRVQSLK
                     VQPKNHKEAKFPSFNITIGYARTTVQRELTRLDAGQCPNTIKIDYSFNEDTCKVDDVLI
                     GDEAISVYSIYDVVAEKYRSILQQVVRNRNRRQDIYDLYYLISQVDFSEEEKIKIFDAF
                     IFKSKERLEGFEYLMDRGALDLAEVIAASEKDYRLLADEIPDGLPDFNDAYSVVRDFFK
                     NLPWG"
                     /name="nucleotidyl transferase AbiEii/AbiGii toxin family
                     protein"
     gene            2054..2707
                     /locus_tag="IFA22_RS25420"
                     /old_locus_tag="KAM330_49890"
     CDS             2054..2707
                     /locus_tag="IFA22_RS25420"
                     /old_locus_tag="KAM330_49890"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139399110.1"
                     /translation="MFTIDGKGSYKCTRAENNDVIVCFIWDEAFFSCVIDGVGCGKNEI
                     CSHIEFELNRFFNADSQPVSTSDFVRFFIDMVGEFKGGGSACCAFVYQCGEEMVYASVG
                     DCRIYWVADRERTVDDTVAQDLIDRGRLKANLYNSHPYRRYVTKTIPRVSIEVRQRKPL
                     MNGDVVVLCSDGAWSILSDDDFYANSAGELVASLERVIGDRGVDNASFVVITKS"
                     /name="hypothetical protein"
     gene            2767..3396
                     /gene="parA"
                     /locus_tag="IFA22_RS25425"
                     /old_locus_tag="KAM330_49900"
     CDS             2767..3396
                     /gene="parA"
                     /locus_tag="IFA22_RS25425"
                     /old_locus_tag="KAM330_49900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017154806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="WP_139399108.1"
                     /translation="MITISVLNQKGGSGKTTIATHLVRAFQIMGGDVLLIDSDPQGSAR
                     DWSAVSDDNTIPVVGIDRPTIERDIKVVSHKDWVVIDGAPQASDLAISAIKASDFVLIP
                     VQPSPYDIWAAADLVDLVKQRIEMTDGHLKAAFVISRAIKNTKIGAEIRGALEGYGLPV
                     LDARVTQSVAYPSSAAIGSTVLDVEPNGNAAKEIMALASELKCKLK"
                     /name="parA"
     gene            3494..3646
                     /locus_tag="IFA22_RS25430"
                     /old_locus_tag="KAM330_49910"
     CDS             3494..3646
                     /locus_tag="IFA22_RS25430"
                     /old_locus_tag="KAM330_49910"
                     /inference="COORDINATES: protein motif:HMM:NF020835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partition protein ParG"
                     /protein_id="WP_232105322.1"
                     /translation="MSDMASSDKVKRVNFDLPEELHKKLKTFAASQGKSIKDVLTEFVE
                     GLAGR"
                     /name="plasmid partition protein ParG"
     gene            complement(3754..4958)
                     /locus_tag="IFA22_RS25435"
     CDS             complement(join(3754..4640,4640..4958))
                     /locus_tag="IFA22_RS25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_100654772.1"
                     /translation="MVEVLTGAERRRRRTPQEKIAIVQQTFEPGMTVSHVARLHDVNAN
                     QLFKWRKQFQEGSLTAITAGEDVVPASELAAAIKQIRELQRLLGKKTMENEILKEAVEY
                     GRGKKMDCACALVAGGRRLSAVSQALNVSRAQLSVRVHRKPGWQDGRHHRQRDDAALLG
                     RIMEAMAELPSYGYRRVWALLRRQSEATEQPVVNAKRVYRVMREHGLLLERKPAAPLAQ
                     QAHKGRVAVKESNRRWCSDGFEFRCDNGEKLRVTFAMDCCDREALDWAASTGGYDSDTV
                     QDVMLRSVERRFGDSLPASPVEWLTDNGSAYRAHETRAFAREIGLEPRTTAVRSPQSNG
                     IAESFVKTMKRDYIEMMPKPDSRTAVGNLAIAFEHYNEHHPHSALGYRSPREFLRSRVS
                     QP"
                     /name="IS3 family transposase"
     gene            5859..6065
                     /locus_tag="IFA22_RS25440"
                     /old_locus_tag="KAM330_49950"
     CDS             5859..6065
                     /locus_tag="IFA22_RS25440"
                     /old_locus_tag="KAM330_49950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017780732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139399121.1"
                     /translation="MRFDKHLIELDGDRVWLLDAVGKRLCDMTDMQLLDLGSRISVEGG
                     LLNFDLEAQKWRECLIALGLELD"
                     /name="hypothetical protein"
     gene            6174..6413
                     /locus_tag="IFA22_RS25445"
                     /old_locus_tag="KAM330_49960"
     CDS             6174..6413
                     /locus_tag="IFA22_RS25445"
                     /old_locus_tag="KAM330_49960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein RepB"
                     /protein_id="WP_139399123.1"
                     /translation="MQEKEAKTMFDMGGFSKAMIVPTPMGSGFHLHLVRFGKAGTEVVE
                     RQRGGWRVFSSIDAAANTANGIGFRRIEIDLSSR"
                     /name="plasmid replication protein RepB"
     gene            complement(6612..7478)
                     /locus_tag="IFA22_RS25450"
                     /old_locus_tag="KAM330_49980"
     CDS             complement(6612..7478)
                     /locus_tag="IFA22_RS25450"
                     /old_locus_tag="KAM330_49980"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_162519627.1"
                     /translation="MHTKVEWDQGKLDAASALAKRKDAVVAIEFIIQVGNQTDWREAPT
                     PECPEGLPIERDLAQFFAPFGEQVKAWADQEFGPENVVSIQLHMDESTPHFHVIATPIK
                     DGKLQAKAWLDGGRKLAGIRRRCHATINEKIPCVYTPNGLGGNPHRPDLRAGTAPVPTL
                     IEKMSGHAKAQKLERENAALQEENAQLKQLLFSRQKGRYSADNVEQAKQATAEAQKAQS
                     ELQKAKGEVKRLTMALDSAQNVIGRQGAEIDKLKGYNRTLSEQNNELEEKLQDLQPKRS
                     RGKGMGL"
                     /name="plasmid recombination protein"
     gene            complement(8046..8258)
                     /locus_tag="IFA22_RS25455"
                     /old_locus_tag="KAM330_50000"
     CDS             complement(8046..8258)
                     /locus_tag="IFA22_RS25455"
                     /old_locus_tag="KAM330_50000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004170235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139399117.1"
                     /translation="MGISDHEYVNFSEDYELNYHLKKVGKAQTEKNRTTLRTMGKELKT
                     LLNKERVTHTEFHSYILKNLSRLDS"
                     /name="hypothetical protein"
     gene            9026..9973
                     /locus_tag="IFA22_RS25460"
                     /old_locus_tag="KAM330_50020"
     CDS             9026..9973
                     /locus_tag="IFA22_RS25460"
                     /old_locus_tag="KAM330_50020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564931.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISAeca1 family transposase"
                     /protein_id="WP_012564931.1"
                     /translation="MKYQQLTEGLRYQIACLRDHGLSQARIAKQLGVHPSTISRELRRN
                     CAGQAYQPQQAHQLATARRLTSQKRTVPSDTIDFVCMALVADWSPEQISGVGDMINCPV
                     SHEWIYRYVARDKANGGTLYRHLRQGHKRYRKGKNSKRSVIPNPVSIDERPAIVNERSR
                     VGDWEVDTVLGKQGSGAIVSLLERKSRLYLVQYVPSKTASAVADTIISMLKPYQAHVHT
                     ITADNGSEFVEHERVAAELEAQVYFAHPYSAWERGQNENSNGLLRQYVPKGSDLSVVTA
                     EALSAVEKRLNFRPRKCLGFKQPQVVFDELLKAA"
                     /name="IS30-like element ISAeca1 family transposase"
     gene            complement(10119..10397)
                     /locus_tag="IFA22_RS25465"
                     /old_locus_tag="KAM330_50030"
     CDS             complement(10119..10397)
                     /locus_tag="IFA22_RS25465"
                     /old_locus_tag="KAM330_50030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_187593338.1"
                     /translation="MNTKASMIIFISILLTACSNNSGVFRTGENQYEVSTRATWELGGR
                     AGANKMAFEEATAHCEKEGKKLKVLDKSEGYGHFEGGTVDLKFSCES"
                     /name="hypothetical protein"
     gene            10883..11866
                     /locus_tag="IFA22_RS25470"
                     /old_locus_tag="KAM330_50040"
     CDS             10883..11866
                     /locus_tag="IFA22_RS25470"
                     /old_locus_tag="KAM330_50040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009157784.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_187593340.1"
                     /translation="MKKSILVLLLAGACWGAPLQVQAKDWENVPLAQVQQAASQGDVDA
                     QFSLGMRYEDDQGGGQDEHKAVTWYRAAAEQGFAAAQFHLGVMYTKGQGVAQDNSQAMA
                     WYRKAAEQGHAQAQSALGGCYFMGNGVAQNTHQAVVWWSKAGAQGDVDAQLSLGSIYLA
                     KKDDRQAAAWYRKAAEQGQVFAQFNLGMMYFNGQGVAKDYSQAVAWSRLAAEQGHVQAQ
                     YFLGWMYTNGLGVVQDDWQALSWIRKAAEQGHVKAQVSLSAMYAQGRGTAPDVKLAYIW
                     SSVAAVNGDADAAHNRDLLAQDLSYAELSDAQAMAGQYAEAYRPQQ"
                     /name="tetratricopeptide repeat protein"
     gene            12009..12278
                     /locus_tag="IFA22_RS25475"
                     /old_locus_tag="KAM330_50050"
     CDS             12009..12278
                     /locus_tag="IFA22_RS25475"
                     /old_locus_tag="KAM330_50050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017411319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039062313.1"
                     /translation="METITHPTLVQLVAAGAVRVVIAVGQPGGWTLLVRYGLAERALAA
                     QRSKQLRVFRKLDTLVLYLQQIGVCRFEVDATGFMGAPRQIRSS"
                     /name="hypothetical protein"
     Typing          6175..6413
                     /name="rep_cluster_2020"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.0"
                     /coverage="100.0"
                     /evalue="2.8e-102"
     Typing          complement(6619..7604)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.264"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gggttttgat agttgttagc atcaaaaaag cgggcttgct ggaagggcat gtggggggct
       61 ctacgagtct ttttttgttt tgtctttttc gattgcaaaa aaagccatga actattggta
      121 gtctagcgtc ctaaggacgc tagactacca atagataact gatttgtcaa attttatttt
      181 aaatacaggg gattagccat gcgatttttt aaaaaaaatt ttgacttcca aagctatccg
      241 atgtaaaagt cctaaggact tttacatcgg atagtgtcac tcatgaaaaa aacaatttcc
      301 ttgcttgagg ccacaaagga gctcatgagg gatgtgcaca aaaagcccgt cataaccact
      361 tacgatttgt actgttatct gctcaggctt tatcaggaca aaacctttga tggcgttaag
      421 atcagggggg tgtctctaga tgctccagag caaagaacct tcgataacat aatatcttcg
      481 ttacagaaat ctggatttat ccacaaagtg gaaggtcttg atgcatatta tctatcaggt
      541 gtagaaaagc ccacgactca gcaaatatta tgcacagttc ttccgttctc atacatatcc
      601 catttaagcg ccatggagtg gcatggaatt acagatagaa taccgtcagt agttcatgtg
      661 actatctgta gcaataaaga attcaaatct ttagaaagaa ataaaatttc aagtgaactc
      721 ggcggcgcaa gtgtcggcaa gtcattttca tttcagcgta aactctctgt aaatgatgtg
      781 aatggaaagc tgattaactt ccatgagatg aaatgcttca cccctcaaaa agaaatatac
      841 ggaacaggag gggttagggt ttcgtctatt gggcatacat tcttgagcat gctgcagcac
      901 ccagaatggt gcggtggatt tagtcatgtt tatgatgttt tttgcgaaaa cgccgaaaaa
      961 cacaaagcgg tgataatcag ggagctggat aaatcaggga ataaaataga tagatctagg
     1021 gctggatata taatggatga ggttctaggc ataaagcatc cagctataga taagtggaag
     1081 ttagaagtaa tgcgaggtgg ctcaagaaag ctgtctccag aaaatgattt ttcaccaaac
     1141 tattctgagg tttggtgttt gtcattaaat ctttaataag gtcacacata tggaagttga
     1201 tataaatgcg tgggttaatg aggcaacacc agagcgggtt agttttaggc aggcggtgca
     1261 tatggttcta catgcagtgg catcgagtga atacctaaaa accagcatgg tcatgaaagg
     1321 agggatgctt cttgggattc gttaccacag ctctaggttt actgaagata ttgatttttc
     1381 ttcaaaaatg aagatttcag atgtgagcac agatgagttc atcggtagca tgaatgacgc
     1441 tttatccctc gctgaggatg atctgggata cggtattcgc tgcagagttc agtcgcttaa
     1501 agttcaacct aaaaatcata aagaggctaa gttcccatca tttaatataa caataggtta
     1561 tgcaagaaca acagtccaac gtgaattaac aaggctggat gctggccaat gtccaaacac
     1621 cattaaaata gactatagct tcaatgaaga tacttgcaaa gtagatgatg ttcttattgg
     1681 tgatgaggct atttcggtat atagcattta tgatgttgtc gctgagaaat acaggtcgat
     1741 attgcagcag gtagttagga atagaaatag gcgtcaggat atttatgatc tttactattt
     1801 gatttcacaa gttgatttta gtgaagagga aaagataaaa atatttgatg cctttatttt
     1861 taaatcaaag gagaggttgg agggttttga atatctgatg gatagaggcg cgcttgatct
     1921 ggcagaagtg attgctgcgt cagagaaaga ttatcgcttg ttagctgatg agatccctga
     1981 tggattgcct gattttaatg atgcttatag cgttgtaaga gattttttta aaaatctccc
     2041 ttggggataa tctatgttta ctatagatgg taaaggtagc tataaatgca ctagagcgga
     2101 aaacaatgat gtcattgttt gttttatttg ggatgaggca ttcttctcat gtgttattga
     2161 tggtgtagga tgtggtaaaa acgaaatttg tagtcatatt gaatttgagt tgaatcgttt
     2221 ttttaatgct gactctcagc cagtgtctac aagtgatttt gttcgttttt ttatcgatat
     2281 ggttggtgag tttaaagggg gtggatccgc ctgttgtgca tttgtgtatc agtgcgggga
     2341 agaaatggtc tatgcgagtg ttggtgactg caggatttac tgggtcgcag atcgtgaacg
     2401 gactgttgat gatacggttg ctcaagactt gatcgataga ggtaggttga aggctaattt
     2461 gtataactct cacccatata ggcgttatgt caccaagacg attccgcgag tcagcattga
     2521 ggtaaggcag aggaagccat tgatgaatgg tgatgtggtg gttttgtgct ctgatggagc
     2581 ttggagcatt ttgagtgatg atgatttcta cgctaattcg gctggtgaac tggttgcgtc
     2641 tttagagaga gtcatagggg ataggggggt tgataatgct tcctttgtgg ttattacaaa
     2701 gtcttgattc tgcgcttgat aggttgagtt tttgatgtaa aactcaaatg agtttttggg
     2761 ggcactatga taacaatatc tgttttgaat cagaagggag ggtctggaaa gaccacaatc
     2821 gcaactcatt tggtcagagc cttccagatc atgggggggg atgtgttgtt gattgactcg
     2881 gatccgcaag gtagcgctag agattggtct gccgttagcg atgacaatac aattcctgtt
     2941 gttggtattg ataggccaac tattgagcgt gatataaagg tcgtgtctca taaggattgg
     3001 gttgtcattg atggtgctcc gcaggcatct gatcttgcta tatccgctat aaaagctagt
     3061 gattttgtct taatcccagt tcagccatcc ccttatgata tttgggccgc tgccgacttg
     3121 gttgatctgg taaagcagag aatagaaatg acggacggtc atttgaaagc tgcttttgtt
     3181 attagtcgag cgataaaaaa caccaaaata ggtgccgaga taagaggggc gctagagggg
     3241 tatgggctcc ccgttttaga tgcaagggtt actcaaagcg ttgcttatcc tagctctgct
     3301 gctatcggat caactgtttt agatgtggaa cctaatggta atgctgcaaa agagattatg
     3361 gctttagcta gtgagttaaa atgcaaactc aaatgagttt atgagtttat gagtttatga
     3421 gtttatgtgg gtagggggtc aagtgagtgc actaaaggct ggtcggccga gtagagaaaa
     3481 aagatcaatg acaatgagtg atatggcttc ttcagataag gtgaaaagag tgaattttga
     3541 ccttcccgaa gagctccata aaaagttaaa aacctttgct gcttcacagg ggaagtcgat
     3601 taaggatgtg ttgactgagt ttgtagaggg attggcaggg cgctgagcgt ggtgttcctt
     3661 cttggctagc aaagttgttc cttgcagaag ttgctgatga tattgaacaa atttggtgga
     3721 tttgacccca taggtccaga cactttttcc cgcttagggt tgtgataccc gactgcgcag
     3781 aaattcccgt ggtgaacggt atcccaaggc gctgtgcggg tggtgctcgt tataatgctc
     3841 aaacgcgata gccaaattgc ctactgctgt tcggctatcc ggttttggca tcatctcgat
     3901 atagtctcgc ttcatcgtct tcacgaagct ttccgctata ccgttactct gtgggcttct
     3961 cactgctgtc gttctgggct ccaacccgat ttctcgtgca aacgcgcggg tctcatgggc
     4021 tcgatacgcc gagccattat cagtcagcca ctccaccggt gatgccggca acgaatcccc
     4081 aaagcgacgt tcgacagacc tcagcatgac atcttgcaca gtgtcactat cgtaaccccc
     4141 agtactggcc gcccagtcca acgcttccct atcgcagcaa tccatggcga acgtgacacg
     4201 tagtttttca ccgttatcgc agcgaaactc gaagccatct gagcaccacc gtcgattgct
     4261 ctccttgacg gcaacacgcc ccttatgtgc ctgctgggcc aatggtgcag ctggttttct
     4321 ttccagcagc aagccatgct cgcgcatgac gcgatatacc cgcttagcgt ttaccacagg
     4381 ttgttctgtt gcctcagact gacgccgtaa cagcgcccag acccggcgat agccatagga
     4441 gggaagctct gccatcgcct ccatgatacg gccaagtaag gctgcatcat cacgctggcg
     4501 atgatgacga ccatcttgcc aacccggttt tcgatgaaca cgaacggata gctgcgcacg
     4561 cgatacatta agagcctggc tcacagcact taatcgtcgt cccccggcaa caagggcgca
     4621 tgcgcaatcc atttttttgc tcgaccatac tccacggctt ctttgaggat ctcgttctcc
     4681 atggtcttct tcccgagcag acgctgaagc tctcggatct gcttgatggc agcagccaac
     4741 tcggaagcag gaacaacatc ctcaccggcg gtgatcgccg tgagagaccc ttcttggaat
     4801 tgcttacgcc atttgaaaag ctggtttgcg ttgacgtcgt gcaagcgagc aacatgagag
     4861 acggtcatcc cgggctcaaa ggtctgctgc acgatggcga tcttttcttg tggagtccgg
     4921 cgtcgacggc gctcggcccc tgttaacact tcgaccatct tctcgttgtg actagtactg
     4981 aacatagctc caagactacc tcttaagtta agaggggcga agtgtctgga tcttcaaggg
     5041 gccaatctat ttggtgttgt tttttcgtat gatcaaagcg gtgctcttgc gctgatcttg
     5101 tcctagcgcg tatgctttca cgcagaaacg aaaaaaccgc cttggcgggc ggtttttcga
     5161 tggtactcag agttggcgct ctttgtacca ggtaacagct tcgagcagga agcggatacc
     5221 ctaatacctt tccagtttag cgccctcggc gcccatcaat caagttttcc tgctcaagcc
     5281 acgttaccta aggccaccac ctttggcgct ttgtcgtacc cgcacggatt ggaagcatga
     5341 gtttgttgcc gtgtgtgggt gtggttggtc gggcagaacg ggggtacctc acatactgcc
     5401 cagcgtgcga cgcgcaccat agccacccat gattcgcaac catggggcac cagcaccgag
     5461 cgcaacgggg aaccaaccac ggccagctcg ataaaaacgg cgcggcagtc cggcccacag
     5521 gtggggacta aagcaggtgc agagtggtga ggtgtgtctc aggagcgcgt gagggggagc
     5581 tgccgcaagc agcctcgata gtcctgagca aggcgagcta tccagcgtaa ctacgtcatt
     5641 tttaacgtag ccatgcagga gggcgagcct atcttgaagc gcagcgaaag ataggctcgc
     5701 ccgtgttttc ggctctcctc ttgaaacaaa aaaaaatcga gatgatcttg gcgaggtggg
     5761 taactgcgcc cgtccgtcca catgccatcg gttcggcttg tggacggcag tagttatcca
     5821 cggaagcggg ttaggtttgg ctctggccgg aggtacgtat gaggttcgac aagcatctga
     5881 tcgagcttga tggtgatcgg gtatggctac tggatgccgt cggcaagcgg ttgtgcgata
     5941 tgacggacat gcagctgctc gatttgggta gccggattag cgtggaaggc ggcctgctca
     6001 acttcgattt ggaggcccag aagtggcggg aatgcctaat tgccttgggc cttgagctgg
     6061 attaaaaatc attttccaga ttcgatgcgt caaatttgga aaatgattgc tttcggctcc
     6121 cgaagatgta caatcaaatt gtttatttga ttcttcaaat tggatatttg acgatgcagg
     6181 agaaagaggc taagacgatg ttcgacatgg gggggttctc caaggcgatg atcgtcccca
     6241 cccccatggg ttctgggttc cacctccact tggtgcgctt tggcaaggcg ggtaccgaag
     6301 tcgtggaacg gcagcgtgga ggctggcggg tgttctcctc catcgatgcg gctgctaaca
     6361 cagccaacgg cattggtttt cgccgcatcg agatagacct tagcagccga taggtccgat
     6421 atatcagact tttgcccagc gcagcaggag ggctagtgcc cccatgcata tcgccccttc
     6481 ccaccatgct ggggcggccc agctgaacac gtcatgcagg ccaagcagcc tcatgaacca
     6541 gccacttgat gagctgcttg cccccttcac tgccagcact gcggccagta gccctacaac
     6601 ctgccatacc ttcataggcc catcccctta ccgcggctcc gctttggctg gagatcctgc
     6661 agtttctcct ccagttcgtt gttctgctcc gaaagcgttc ggttatagcc tttgagcttg
     6721 tcgatctctg ccccttgtcg gcctatcacg ttctgggcgc tatcgagcgc catggtgagc
     6781 cgcttaacct cccctttcgc cttctggagc tcgctctggg ccttctgtgc ctctgcggta
     6841 gcctgcttgg cctgctccac gttgtccgcg ctatagcgac ccttctggcg gctaaacagg
     6901 agctgcttga gctgggcatt ctcctcctgc agtgcggcgt tctcccgctc cagcttctgg
     6961 gctttggcgt ggccgctcat tttctcgatc agggtcggga caggggcggt gccggcgcgc
     7021 aggtctggcc ggtgcgggtt tccccccaga ccgttggggg tgtacacgca ggggatcttt
     7081 tcgttgatcg tggcgtggca tcggcgccgg atccccgcca gcttccggcc gccatctagc
     7141 cacgctttcg cctgcagctt gccgtctttg atcggggtgg caatgacatg gaagtgcggg
     7201 gtgctctcgt ccatatgcag ctggatagat accacgttct cggggccgaa ctcctgatcg
     7261 gcccatgcct tgacctgctc cccgaacggg gcaaagaact gcgccagatc gcgctcgata
     7321 ggcagccctt cagggcactc tggtgtgggg gcttctcgcc agtcggtttg gttgccgacc
     7381 tggatgatga actcgatcgc caccacggca tctttgcgct tggccagtgc cgaggccgca
     7441 tcgagtttgc cctgatccca ctcgaccttg gtgtgcatcg ggctctctgt aaaccatgcc
     7501 cgttttggaa gctggctgct ggtttcgtgg aggcggaggt tgtgtccctg agccctgcca
     7561 atgtcggctt tgctcatctt ggctacggcg aacgagaact tcatcggggc tccgagggtg
     7621 gcagctttgg atttcaaagt gtactcctta cactttgcta cgcaaagtag gagcccttcg
     7681 gggcgaaaaa gacctgccgc gctacgcttg ccgtctcccg atctggagga ggcagactgc
     7741 gctagcctcc acctgttcgg gaggtttccg cgaaaagcgt cattacaggt cgagtatgtc
     7801 gtgatgccac catgcaggca tcaaactgca gaggcaaaga ggtgcttgta tgagggactg
     7861 ggtgttggca gtgctgctcc tgataggagg gaatagcatg gaacataact tgggggtggc
     7921 ggcggtcatc gttgctgtgg cgctcatgct taaggaatcc aatcgaatcg atccctgatc
     7981 cacatgaaag tggggccact ggccccacag agtttcgcat gcgaaatttt gaggctaaga
     8041 gaccattagg aatccaagcg actgaggttt ttcagaatgt aggaatgaaa ctcggtgtga
     8101 gtaactcgct ccttgttgag cagtgttttc agctctttgc ccatcgtgcg caacgtggtt
     8161 ctgtttttct ctgtttgagc cttacccacc tttttcaggt gatagttcag ctcgtagtct
     8221 tcgctgaagt taacgtactc gtgatcgctg atacccatga catccttccc ttggtcatcg
     8281 agccttaagg attaaggttc gttcagacaa gctctgggcg acagcactga ttttcaagtg
     8341 gctctttctt gattttgtga gtagggccac tggccccacc ttttgtaaat ttttcaggta
     8401 ccaaagaaaa ctacgagaat cacctgttct tggtagttat cggatgaaac cggatccgct
     8461 gctctggccg gtaaagatcc cgtccaaacc gttactaatt ttcagcacca aaaggcgcgg
     8521 tgttgggctt caacactgcg ctttttcggc tgtttgggga gtcttctgat tgggtggcaa
     8581 gttttcgcat gcgaaaactt caattgaaca taaaaggcgt tatgcgctac ccgatcggct
     8641 cgtgcacata aagggaggtt acgttaaacg ggcgctcctt tttgcgttat tacagggttt
     8701 attttttatg tgagccgtac ttattgcctg ttctaaaatt tttgtaagac ccgcctttat
     8761 gtgacgatcc ctttccgcca gagtaatgtc cgttatggct aattgtatga tggcttcctg
     8821 tgtaataaac cctcgacccc tttgcaaaag cagcctgcgc accagcgaag agaaatacaa
     8881 atataagcgc cgcaattttg aaatgtttgt tcattagccg tgaattcaga taataagtgc
     8941 aatgcccata tggtgcatag gagagaggcg gtcaactacc agttagggta cgcttcttct
     9001 cgccaaagaa acaaagcgat acaccatgaa ataccagcag ttgaccgagg ggctacgata
     9061 ccagattgcc tgcctccgtg accatggcct gtcacaggcc cgcattgcca aacagctcgg
     9121 ggttcatcca tcgactatca gtcgtgagct tcgtcgtaat tgtgctgggc aggcctatca
     9181 gccgcagcag gctcaccaac ttgccacggc acgacggctg acgagtcaga agcgtactgt
     9241 cccgtctgat accatcgact ttgtttgcat ggcgctggtc gcggactgga gtcccgagca
     9301 aatcagtggc gttggagaca tgattaactg cccggttagc cacgagtgga tataccgcta
     9361 tgtggcacgc gacaaggcca acggtggcac gctataccgg catctgcgtc agggtcacaa
     9421 acgctatcgc aagggtaaga acagcaaacg cagtgtcatt cccaatccgg tatcgattga
     9481 tgaacgccct gcgatagtca atgagcggag ccgtgtggga gattgggaag tcgataccgt
     9541 gttgggtaag caaggcagcg gtgcaatagt gagtctgttg gagcgtaaga gccggctcta
     9601 cctggttcaa tacgttccca gcaaaacagc cagcgcggtg gcggacacca ttatcagcat
     9661 gctgaaaccc taccaggcac atgtccatac catcacagct gacaatggta gcgagtttgt
     9721 cgaacatgaa cgtgttgcag cagagctgga agcgcaggtg tactttgcgc atccatacag
     9781 tgcatgggaa cggggacaga atgagaatag caatggtctg ctgcggcaat atgtgcccaa
     9841 aggcagtgat ttgagtgtgg tgacagcaga ggcactgtca gccgtcgaaa agcggctgaa
     9901 cttcaggccg cgcaaatgct tgggatttaa acagccacag gtggtttttg acgagcttct
     9961 gaaagcagcc tgatttttaa tcgggaattg cacttcgaag ttgaattcgc ggctacctca
    10021 agaaacacat aagctattta gcctctgcaa gtccgcaatg ggcactgccc agtgcgtaag
    10081 atcccatata gcattgctct ggttcaatca ctttaaaatc atgattcgca gctaaatttt
    10141 aaatcgactg taccaccctc gaaatggcca tacccttcac ttttatcaag tactttcaat
    10201 tttttacctt ctttttcaca gtgagctgtt gcttcttcga atgccatttt gttagctcct
    10261 gctctgccac ctagctccca tgtcgctcgc gttgacactt catattgatt ttctccagtg
    10321 cgaaaaactc ctgaattatt tgagcaagca gttaataaaa ttgaaatgaa aataatcatt
    10381 gatgctttgg tgttcatgat cactccgtga gttctgaatg atattcgttt tggtcgtact
    10441 gccaacaagt tgaagtactg tacagtgatc gtgttaagcc tgccatctcc ccattgcttt
    10501 tgcgcgaatg tgtgactcct gcgtattccg gtgaagttga acagtgattc cggaatctgt
    10561 gaacaccatt tccggaaagt atgcggacag ggattcttcg agcagaagct gggctttgag
    10621 gggaggcatc ttttcgactc actgaacaag ccagcagcca agcgcagcgc ggcagttgtg
    10681 gatccaagac gttggaacag gacggttcat ctgacatcag agtacgtgct ctgaggtgtc
    10741 cggaatcggt gtacacgcaa gccggaatca gcgttcattt taaaccggaa taggtgttca
    10801 ttttcgaccg gaatatgcaa ctccagcaag ttattgatac ggacaccgat tacaatgcga
    10861 aaaattcaat ggagtgatag atatgaaaaa atcgatatta gtgctgctgt tagccggtgc
    10921 ttgttggggg gcaccgctac aggttcaggc gaaagattgg gaaaatgtac cacttgccca
    10981 agtgcaacaa gcggcctcgc aaggggatgt ggacgctcag ttcagcctcg gcatgagata
    11041 cgaagacgac caaggggggg gacaagatga gcacaaggct gtgacatggt accgcgcagc
    11101 ggccgaacaa ggttttgccg ctgctcagtt ccacctagga gtgatgtaca ccaaaggcca
    11161 aggggtggca caagataata gccaagccat ggcctggtac cgcaaggcgg ccgagcaagg
    11221 gcatgcccag gcacagtcag ctctcggcgg gtgttatttt atgggcaatg gtgtggcaca
    11281 aaatacccac caagctgtgg tctggtggag caaggcgggc gcgcaagggg atgtggacgc
    11341 gcagctcagc ctcggctcga tatatttggc aaaaaaagat gaccgccaag ccgcagcctg
    11401 gtaccgcaag gctgccgagc aagggcaggt gtttgcgcaa ttcaaccttg gaatgatgta
    11461 cttcaatggt caaggggtgg ctaaagatta tagccaagca gtggcctgga gccgtctagc
    11521 ggctgagcaa gggcatgtcc aggcacagta cttccttggc tggatgtaca ccaatggttt
    11581 aggggtggtg caagatgact ggcaagcgtt gtcctggatc cgcaaggcgg cagaacaagg
    11641 gcatgtcaag gcacaggtca gcctcagtgc gatgtacgcc cagggccgag ggacggcacc
    11701 ggatgtcaag cttgcttaca tctggagttc ggtggcggcg gtcaatgggg atgccgatgc
    11761 ggctcataac cgtgacctct tagctcaaga cttgtcgtat gcggaattga gcgatgcaca
    11821 agcgatggca ggccagtatg ctgaggcgta ccgcccgcag cagtaaccac ctctgtcagt
    11881 gcgcataagg gggcgttacg ttaaatcgga cgcccttttt gctgctggtc atcctgcaag
    11941 atgacttgtt ttgcgaggcg ttatctccca tcctgcgccg cactatccct tgtctcggaa
    12001 tctcccccat ggaaaccatt acccatccca ccctggtaca actggtggcg gccggtgctg
    12061 ttcgtgtcgt gatcgcagtt ggccaacctg gcggctggac gctgctggtg cgctacggtc
    12121 tagccgaacg tgccctggcc gcccagcgca gcaagcagct cagagttttt cgcaagctcg
    12181 ataccctggt tctctacctg cagcagatcg gggtctgccg gttcgaggtg gatgccacgg
    12241 gcttcatggg tgccccgaga cagatcagga gctcttgaac ataaaaggcg ttatgcgcac
    12301 cgatttaacg t
//
