LOCUS       NZ_AP023302             7257 bp    DNA     circular CON 23-SEP-2023
DEFINITION  Salmonella enterica subsp. enterica serovar 4,[5],12:i:- strain
            L-4445 plasmid pSAL4445-2, complete sequence.
ACCESSION   NZ_AP023302
VERSION     NZ_AP023302.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00234603
            Sequence Read Archive: DRR236424
            Assembly: GCF_019670285.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar 4,[5],12:i:-
  ORGANISM  Salmonella enterica subsp. enterica serovar 4,[5],12:i:-
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Arai,N., Akiba,M. and Kuroda,M.
  TITLE     complete genome sequences of Salmonella enterica subsp. enterica
            serovar 4,[5],12:i:- str. L-4445
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7257)
  AUTHORS   Sekizuka,T., Arai,N., Akiba,M. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Canu v. 1.4; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 164x
            Sequencing Technology             :: Sequel; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/23/2023 04:00:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,977
            CDSs (total)                      :: 4,854
            Genes (coding)                    :: 4,713
            CDSs (with protein)               :: 4,713
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 42 of 141
            Pseudo Genes (incomplete)         :: 110 of 141
            Pseudo Genes (internal stop)      :: 19 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023302.1.
            Annotated by DFAST v.1.0.8 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Salmonella enterica subsp. enterica serovar
                     4,[5],12:i:-"
                     /mol_type="genomic DNA"
                     /strain="L-4445"
                     /serovar="4,[5],12:i:-"
                     /isolation_source="Bos taurus"
                     /sub_species="enterica"
                     /db_xref="taxon:440524"
                     /plasmid="pSAL4445-2"
                     /geo_loc_name="Japan"
                     /collection_date="2014"
     gene            206..>739
                     /locus_tag="K5630_RS24945"
                     /pseudo=""
     CDS             206..>739
                     /locus_tag="K5630_RS24945"
                     /inference="COORDINATES: protein motif:HMM:NF015354.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            849..1262
                     /locus_tag="K5630_RS24950"
     CDS             849..1262
                     /locus_tag="K5630_RS24950"
                     /inference="COORDINATES: protein motif:HMM:NF036929.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            1307..2230
                     /locus_tag="K5630_RS24955"
     CDS             1307..2230
                     /locus_tag="K5630_RS24955"
                     /inference="COORDINATES: protein motif:HMM:NF028103.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_014344452.1"
                     /translation="MGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDV
                     YIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELR
                     GQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAG
                     PALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDD
                     LSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQ
                     LARAELARAPAPRQRGMDRGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            2291..2503
                     /locus_tag="K5630_RS24645"
                     /old_locus_tag="SEL4445_P20011"
     CDS             2291..2503
                     /locus_tag="K5630_RS24645"
                     /old_locus_tag="SEL4445_P20011"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            2505..2711
                     /gene="urfF"
                     /locus_tag="K5630_RS24650"
                     /old_locus_tag="SEL4445_P20012"
     CDS             2505..2711
                     /gene="urfF"
                     /locus_tag="K5630_RS24650"
                     /old_locus_tag="SEL4445_P20012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     gene            2741..3580
                     /locus_tag="K5630_RS24655"
                     /old_locus_tag="SEL4445_P20013"
     CDS             2741..3580
                     /locus_tag="K5630_RS24655"
                     /old_locus_tag="SEL4445_P20013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            3567..4418
                     /gene="repC"
                     /locus_tag="K5630_RS24660"
                     /old_locus_tag="SEL4445_P20014"
     CDS             3567..4418
                     /gene="repC"
                     /locus_tag="K5630_RS24660"
                     /old_locus_tag="SEL4445_P20014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            4643..5026
                     /locus_tag="K5630_RS24665"
                     /old_locus_tag="SEL4445_P20015"
     CDS             4643..5026
                     /locus_tag="K5630_RS24665"
                     /old_locus_tag="SEL4445_P20015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_221056297.1"
                     /translation="MKMNIYKSIKYISILLLFVLFVGVGQLGQFFNSNYDVRKYALESW
                     AGEDKERQDQVSVFIQACLIGQKANKESFKKEMELKQEGILHEPTIAICAKKYGYTELY
                     EVVNNADTVLQSAAWPLSMVADL"
                     /name="hypothetical protein"
     gene            5350..6204
                     /gene="estX"
                     /locus_tag="K5630_RS24670"
                     /old_locus_tag="SEL4445_P20016"
     CDS             5350..6204
                     /gene="estX"
                     /locus_tag="K5630_RS24670"
                     /old_locus_tag="SEL4445_P20016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold putative hydrolase EstX"
                     /protein_id="WP_130045120.1"
                     /translation="MKEKVVVDKAISLHTESFGDPAHEPIILIMGAMASAVWWPDEFCS
                     QLAKMGRYVIRYDHRDTGKSTSYEPGQAPYSVEELADDVVRVIDGYGLEAAHLVGMSLG
                     GFLSQLVALKYPKRVKSLTLIASERLADADPDMPAFDPAIIEYHQRAESLDWSDRDAVV
                     AYQVGAWRIISGTAHAFDAEKIQNIAELDFDRTPNILTTFNHTTSGGGERWLERWLGRL
                     NEIAVPTLIIHGTEDPVLPYVHGLALKDAIRGSKMLTLEGTGHELHHEDWPRIIQAIKG
                     QTS"
                     /name="estX"
     gene            complement(6874..7158)
                     /locus_tag="K5630_RS24675"
                     /old_locus_tag="SEL4445_P20017"
     CDS             complement(6874..7158)
                     /locus_tag="K5630_RS24675"
                     /old_locus_tag="SEL4445_P20017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     Typing          7113..7257
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="92.0"
                     /evalue="2.99e-70"
     Typing          2759..3580
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          102..2227
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtttctcttc gagaaactgg cctaacggcc acccttcggg cggtgcgctc tccgagggcc
       61 attgcatgga gccgaaaagc aaaagcaaca gcgaggcagc atggcgattt atcaccttac
      121 ggcgaaaacc ggcagcaggt cgggcggcca atcggccagg gccaaggccg actacatcca
      181 gcgcgaaggc aagtatgccc gcgacatgga tgaagtcttg cacgccgaat ccgggcacat
      241 gccggagttc gtcgagcggc ccgccgacta ctgggatgct gccgacctgt atgaacgcgc
      301 caatgggcgg ctgttcaagg aggtcgaatt tgccctgccg gtcgagctga ccctcgacca
      361 gcagaaggcg ctggcgtccg agttcgccca gcacctgact ggtgccgagc gcctgccgta
      421 tacgctggcc atccatgccg gtggcggcga gaacccgcac tgccacctga tgatctccga
      481 gcggatcaat gacggcatcg agcggcccgc cgctcagtgg ttcaagcggt acaacggcaa
      541 gaccccggag aagggcgggg cacagaagac cgaagcgctc aagcccaagg catggcttga
      601 gcagacccgc gaggcatggg ccgaccatgc caaccgggca ttagagcggg ctggccacga
      661 cgcccgcatt gaccacagaa cacttgaggc gcagggcatc gagcgcctgc ccggtgttca
      721 cctggggccg aacgtggtgg agatggaagg ccggggcatc cgcaccgacc gggcagacgt
      781 ggccctgaac atcgacaccg ccaacgccca gatcatcgac ttacaggaat accgggaggc
      841 aatagaccat gaacgcaatc gacagagtga agaaatccag aggcatcaac gagttagcgg
      901 agcagatcga accgctggcc cagagcatgg cgacactggc cgacgaagcc cggcaggtca
      961 tgagccagac ccagcaggcc agcgaggcgc aggcggcgga gtggctgaaa gcccagcgcc
     1021 agacaggggc ggcatgggtg gagctggcca aagagttgcg ggaggtagcc gccgaggtga
     1081 gcagcgccgc gcagagcgcc cggagcgcgt cgcgggggtg gcactggaag ctatggctaa
     1141 ccgtgatgct ggcttccatg atgcctacgg tggtgctgct gatcgcatcg ttgctcttgc
     1201 tcgacctgac gccactgaca accgaggacg gctcgatctg gctgcgcttg gtggcccgat
     1261 gaagaacgac aggactttgc aggccatagg ccgacagctc aaggccatgg gctgtgagcg
     1321 cttcgatatc ggcgtcaggg acgccaccac cggccagatg atgaaccggg aatggtcagc
     1381 cgccgaagtg ctccagaaca cgccatggct caagcggatg aatgcccagg gcaatgacgt
     1441 gtatatcagg cccgccgagc aggagcggca tggtctggtg ctggtggacg acctcagcga
     1501 gtttgacctg gatgacatga aagccgaggg ccgggagcct gccctggtag tggaaaccag
     1561 cccgaagaac tatcaggcat gggtcaaggt ggccgacgcc gcaggcggtg aacttcgggg
     1621 gcagattgcc cggacgctgg ccagcgagta cgacgccgac ccggccagcg ccgacagccg
     1681 ccactatggc cgcttggcgg gcttcaccaa ccgcaaggac aagcacacca cccgcgccgg
     1741 ttatcagccg tgggtgctgc tgcgtgaatc caagggcaag accgccaccg ctggcccggc
     1801 gctggtgcag caggctggcc agcagatcga gcaggcccag cggcagcagg agaaggcccg
     1861 caggctggcc agcctcgaac tgcccgagcg gcagcttagc cgccaccggc gcacggcgct
     1921 ggacgagtac cgcagcgaga tggccgggct ggtcaagcgc ttcggtgatg acctcagcaa
     1981 gtgcgacttt atcgccgcgc agaagctggc cagccggggc cgcagtgccg aggaaatcgg
     2041 caaggccatg gccgaggcca gcccagcgct ggcagagcgc aagcccggcc acgaagcgga
     2101 ttacatcgag cgcaccgtca gcaaggtcat gggtctgccc agcgtccagc ttgcgcgggc
     2161 cgagctggca cgggcaccgg caccccgcca gcgaggcatg gacaggggcg ggccagattt
     2221 cagcatgtag tgcttgcgtt ggtactcacg cctgttatac tatgagtact cacgcacaga
     2281 agggggtttt atggaatacg aaaaaagcgc ttcagggtcg gtctacctga tcaaaagtga
     2341 caagggctat tggttgcccg gtggctttgg ttatacgtca aacaaggccg aggctggccg
     2401 cttttcagtc gctgatatgg ccagccttaa ccttgacggc tgcaccttgt ccttgttccg
     2461 cgaagacaag cctttcggcc ccggcaagtt tctcggtgac tgatatgaaa gaccaaaagg
     2521 acaagcagac cggcgacctg ctggccagcc ctgacgctgt acgccaagcg cgatatgccg
     2581 agcgcatgaa ggccaaaggg atgcgtcagc gcaagttctg gctgaccgac gacgaatacg
     2641 aggcgctgcg cgagtgcctg gaagaactca gagcggcgca gggcgggggt agtgaccccg
     2701 ccagcgccta accaccaact gcctgcaaag gaggcaatca atggctaccc ataagcctat
     2761 caatattctg gaggcgttcg cagcagcgcc gccaccgctg gactacgttt tgcccaacat
     2821 ggtggccggt acggtcgggg cgctggtgtc gcccggtggt gccggtaaat ccatgctggc
     2881 cctgcaactg gccgcacaga ttgcaggcgg gccggatctg ctggaggtgg gcgaactgcc
     2941 caccggcccg gtgatctacc tgcccgccga agacccgccc accgccattc atcaccgcct
     3001 gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa gccgtggctg acggcctgct
     3061 gatccagccg ctgatcggca gcctgcccaa catcatggcc ccggagtggt tcgacggcct
     3121 caagcgcgcc gccgagggcc gccgcctgat ggtgctggac acgctgcgcc ggttccacat
     3181 cgaggaagaa aacgccagcg gccccatggc ccaggtcatc ggtcgcatgg aggccatcgc
     3241 cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc agcaagggcg cggccatgat
     3301 gggcgcaggc gaccagcagc aggccagccg gggcagctcg gtactggtcg ataacatccg
     3361 ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc gaggaatggg gtgtggacga
     3421 cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag gccaactatg gcgcaccgtt
     3481 cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc aagcccgccg tgctggagag
     3541 gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa gaacaagcac agcctcagcc
     3601 acgtccggca cgacccggcg cactgtctgg cccccggcct gttccgtgcc ctcaagcggg
     3661 gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta cggcgacggc aagcggatcg
     3721 agttcagcgg cccggagccg ctgggcgctg atgatctgcg catcctgcaa gggctggtgg
     3781 ccatggctgg gcctaatggc ctagtgcttg gcccggaacc caagaccgaa ggcggacggc
     3841 agctccggct gttcctggaa cccaagtggg aggccgtcac cgctgatgcc atggtggtca
     3901 aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga ggtcgatagt ggtggggcgc
     3961 tcaagcacat acaggactgc atcgagcgcc tttggaaggt atccatcatc gcccagaatg
     4021 gccgcaagcg gcaggggttt cggctgctgt cggagtacgc cagcgacgag gcggacgggc
     4081 gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt catgggtggc ggccagcatg
     4141 tgcgcatcag catggacgag gtgcgggcgc tggacagcga aaccgcccgc ctgctgcacc
     4201 agcggctgtg tggctggatc gaccccggca aaaccggcaa ggcttccata gataccttgt
     4261 gcggctatgt ctggccgtca gaggccagtg gttcgaccat gcgcaagcgc cgccagcggg
     4321 tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac ggtaaccgag ttcgcggcgg
     4381 gcaagtacga catcacccgg cccaaggcgg caggctgacc ccccccactc tattgtaaac
     4441 aagacatttt tatctttata aaacaattgg ttggtgtaga agtttaataa agatggtaac
     4501 taaacgcgcc tgcaagccgc attgtgttgc ttagtataat tcgcacaaaa aacaaagttg
     4561 gtaactttta ctgattttca aaaaccatga cgttagaagt gtactaataa agttggtaac
     4621 tttcaaagag agcaatttga gcatgaagat gaatatatat aaatcaatta aatacatatc
     4681 aattttgttg ttgtttgtat tgtttgttgg tgtaggacag cttggtcagt tttttaattc
     4741 aaattatgac gttagaaaat atgcgcttga gagctgggct ggtgaagata aagagcgaca
     4801 ggatcaggtg tcagtattta tccaagcttg cctaattgga cagaaagcta ataaagaaag
     4861 cttcaaaaaa gaaatggaac tgaaacagga aggcattttg catgagccaa ctattgcaat
     4921 ctgtgcaaag aaatatggtt atacggagct atatgaggtt gttaataatg cggatacggt
     4981 tttacagtct gccgcgtggc ctttgtctat ggtggccgat ttatgactgc cgtttcttga
     5041 gcggcgattg cagcgaagct taaagactaa tgcggtagtt accaacttta ttagtaagtt
     5101 accaatttta ttatgttgtt accgacttta ttgtcatgtc acagttgggg atgtttgagc
     5161 gcaattaaca ggcagccaaa actggcaaaa tgagcatgct cgttttgcca gatttgaatt
     5221 gattgcggca atggaaaaag taaggtagaa tcaggcactt aaataataaa tagagtaggg
     5281 atgcccaaag acaaaatgag catgcctatg aataaaatgt tgtacgtaaa actcgaaggc
     5341 aattttagta tgaaagaaaa ggtagttgtt gataaagcga tttcactcca taccgaatca
     5401 ttcggcgacc cggcccatga acccattatc ctgatcatgg gggcaatggc gtctgcggtg
     5461 tggtggcctg atgagttttg ctcccaacta gccaaaatgg gtcgctatgt gatccggtac
     5521 gaccaccgtg ataccgggaa atcaacaagc tatgagccag gtcaagctcc atattccgtt
     5581 gaagaattag cagatgatgt ggttcgcgtc attgatggtt atggtctgga agctgctcat
     5641 ttagtcggca tgtctttggg gggatttctt tcccagcttg tagctctcaa gtatccgaaa
     5701 cgtgtgaaga gcttgacgct gattgcttca gaacggcttg cagatgcaga tcctgatatg
     5761 cccgcttttg atcctgccat cattgagtat caccaacggg cggaatcgct ggattggtct
     5821 gatagagatg ctgtcgtcgc gtatcaggtc ggagcgtggc gaatcatctc aggtactgcg
     5881 catgcttttg acgctgagaa gatacaaaat atcgctgagt tagattttga tcgcactccg
     5941 aatatcctga caacattcaa ccacactact tcaggtggtg gcgagagatg gctcgagaga
     6001 tggctcggga gattaaatga gatagctgta ccaactttga tcattcacgg cacggaggat
     6061 cctgtacttc cttatgtgca tgggttggca ctgaaagatg cgattcgtgg ttcaaaaatg
     6121 ctgacactcg aaggcacggg acatgagttg catcatgaag actggccgag gattatccag
     6181 gcgattaagg ggcaaacgtc atagctatcg gttgcaccgt acaacaagtt gctgcaccgt
     6241 aaaaattact cgctgcgctc gcaattttcc ggtgagcaag gcgttaaaca tcatgaggga
     6301 agcggtgatc gctgaggcat gttccgcgtc cttgcaatac tgtgtttaca tacagtctat
     6361 cgcttagcgg aaagttcttt taccctcagc cgaaatgcct gccgttgcta gacattgcca
     6421 gccagtgccc gtcactcccg tactaactgt cacgaacccc tgcaataact gtcacgcccc
     6481 cctgcaataa ctgtcacgaa cccctgcaat aactgtcacg cccccaaacc tgcaaaccca
     6541 gcaggggcgg gggctggcgg ggtgttggaa aaatccatcc atgattatct aagaataatc
     6601 cactaggcgc ggttatcagc gcccttgtgg ggcgctgctg cccttgccca atatgcccgg
     6661 ccagaggccg gatagctggt ctattcgctg cgctaggcta cacaccgccc caccgctgcg
     6721 cggcaggggg aaaggcgggc aaagcccgct aaaccccaca ccaaaccccg cagaaatacg
     6781 ctggagcgct tttagccgct ttagcggcct ttccccctac ccgaagggtg ggggcgcgtg
     6841 tgcagccccg cagggcctgt ctcggtcgat cattcagccc ggctcatcct tctggcgtgg
     6901 cggcagaccg aacaaggcgc ggtcgtggtc gcgttcaagg tacgcatcca ttgccgccat
     6961 gagccgatcc tccggccact cgctgctgtt caccttggcc aaaatcatgg cccccaccag
     7021 caccttgcgc cttgtttcgt tcttgcgctc ttgctgctgt tcccttgccc gcacccgctg
     7081 aatttcggca ttgattcgcg ctcgttgttc ttcgagcttg gccagccgat ccgccgcctt
     7141 gttgctcccc ttaaccatct tgacacccca ttgttaatgt gctgtctcgt aggctatcat
     7201 ggaggcacag cggcggcaat cccgacccta ctttgtaggg ggagggcgca cttaccg
//
