LOCUS       NZ_AP023293             7618 bp    DNA     circular CON 23-SEP-2023
DEFINITION  Salmonella enterica subsp. enterica serovar 4,[5],12:i:- strain
            L-4233 plasmid pSAL4233-1, complete sequence.
ACCESSION   NZ_AP023293
VERSION     NZ_AP023293.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00097500
            Sequence Read Archive: DRR107043, DRR107044
            Assembly: GCF_019670225.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar 4,[5],12:i:-
  ORGANISM  Salmonella enterica subsp. enterica serovar 4,[5],12:i:-
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Arai,N., Akiba,M. and Kuroda,M.
  TITLE     complete genome sequences of Salmonella enterica subsp. enterica
            serovar 4,[5],12:i:- str. L-4233
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7618)
  AUTHORS   Sekizuka,T., Arai,N., Akiba,M. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Canu v. 1.4; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 210x
            Sequencing Technology             :: Sequel; NextSeq500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/23/2023 03:31:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,815
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 32 of 114
            Pseudo Genes (incomplete)         :: 92 of 114
            Pseudo Genes (internal stop)      :: 19 of 114
            Pseudo Genes (multiple problems)  :: 27 of 114
            Pseudo Genes (short protein)      :: 1 of 114
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023293.1.
            Annotated by DFAST v.1.0.8 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Salmonella enterica subsp. enterica serovar
                     4,[5],12:i:-"
                     /mol_type="genomic DNA"
                     /strain="L-4233"
                     /serovar="4,[5],12:i:-"
                     /isolation_source="Bos taurus"
                     /sub_species="enterica"
                     /db_xref="taxon:440524"
                     /plasmid="pSAL4233-1"
                     /geo_loc_name="Japan"
                     /collection_date="2014"
     gene            206..>739
                     /locus_tag="K5617_RS24130"
                     /pseudo=""
     CDS             206..>739
                     /locus_tag="K5617_RS24130"
                     /inference="COORDINATES: protein motif:HMM:NF015354.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            849..1262
                     /locus_tag="K5617_RS24135"
     CDS             849..1262
                     /locus_tag="K5617_RS24135"
                     /inference="COORDINATES: protein motif:HMM:NF036929.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            1307..2230
                     /locus_tag="K5617_RS24140"
     CDS             1307..2230
                     /locus_tag="K5617_RS24140"
                     /inference="COORDINATES: protein motif:HMM:NF028103.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_014344452.1"
                     /translation="MGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDV
                     YIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELR
                     GQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAG
                     PALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDD
                     LSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQ
                     LARAELARAPAPRQRGMDRGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            2291..2503
                     /locus_tag="K5617_RS23895"
                     /old_locus_tag="SEL4233_P0020"
     CDS             2291..2503
                     /locus_tag="K5617_RS23895"
                     /old_locus_tag="SEL4233_P0020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            2505..2711
                     /gene="urfF"
                     /locus_tag="K5617_RS23900"
                     /old_locus_tag="SEL4233_P0030"
     CDS             2505..2711
                     /gene="urfF"
                     /locus_tag="K5617_RS23900"
                     /old_locus_tag="SEL4233_P0030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     gene            2741..3580
                     /locus_tag="K5617_RS23905"
                     /old_locus_tag="SEL4233_P0040"
     CDS             2741..3580
                     /locus_tag="K5617_RS23905"
                     /old_locus_tag="SEL4233_P0040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            3567..4418
                     /gene="repC"
                     /locus_tag="K5617_RS23910"
                     /old_locus_tag="SEL4233_P0050"
     CDS             3567..4418
                     /gene="repC"
                     /locus_tag="K5617_RS23910"
                     /old_locus_tag="SEL4233_P0050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            4800..6140
                     /locus_tag="K5617_RS23915"
                     /old_locus_tag="SEL4233_P0060"
     CDS             4800..6140
                     /locus_tag="K5617_RS23915"
                     /old_locus_tag="SEL4233_P0060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="WP_038989331.1"
                     /translation="MSILQFLIAVDVTVVNVALPSIGNDLGVDARGLTWVVVGYTITGG
                     GLLMLGGRLGDVFGRRRLLLIGTAIFGAASLMAGLAETFPLLVGARLLQGVGEALALPA
                     AMATIVLLFPEGRARTRALSVWAAVASCGLVLGFVLSGIITEYLGWRWIFLIAVPFVLF
                     VLIASVALLPRDRSQERAPLDILGSILLTSAPLLFAFGVVEAGDSNGDVPWSSIAALVG
                     AAAAAALFVKVERRAANPLVPLTFFRNRTRVRANLATALLSAALSTSFLLFTFYLQDGL
                     GLSPLESGLLLLPLAVALVVAVTVVPRLLGRWGARACIVVGLSSAGAGMIVIAAAAQLE
                     APAWLLTPAMLFIAAGMGFGLVGLQYAAVTEVTDDDAGVASGVQRAADQLGGSAGVTVY
                     VGLGFASALHGVSPYVLSSGLAIIGLCLATAIVQRIVIVTQEQQTRT"
                     /name="MFS transporter"
     gene            6186..>6530
                     /locus_tag="K5617_RS23920"
                     /old_locus_tag="SEL4233_P0070"
                     /pseudo=""
     CDS             6186..>6530
                     /locus_tag="K5617_RS23920"
                     /old_locus_tag="SEL4233_P0070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019310991.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            <6543..>6698
                     /locus_tag="K5617_RS23925"
                     /old_locus_tag="SEL4233_P0080"
                     /pseudo=""
     CDS             <6543..>6698
                     /locus_tag="K5617_RS23925"
                     /old_locus_tag="SEL4233_P0080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017245253.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]; GO:0020037 - heme binding [Evidence IEA]"
                     /GO_process="GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /name="cbb3-type cytochrome c oxidase subunit I"
     gene            complement(7236..7520)
                     /locus_tag="K5617_RS23930"
                     /old_locus_tag="SEL4233_P0090"
     CDS             complement(7236..7520)
                     /locus_tag="K5617_RS23930"
                     /old_locus_tag="SEL4233_P0090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     Typing          7475..7618
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="92.0"
                     /evalue="1.07e-69"
     Typing          2759..3580
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          102..2227
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtttctcttc gagaaactgg cctaacggcc acccttcggg cggtgcgctc tccgagggcc
       61 attgcatgga gccgaaaagc aaaagcaaca gcgaggcagc atggcgattt atcaccttac
      121 ggcgaaaacc ggcagcaggt cgggcggcca atcggccagg gccaaggccg actacatcca
      181 gcgcgaaggc aagtatgccc gcgacatgga tgaagtcttg cacgccgaat ccgggcacat
      241 gccggagttc gtcgagcggc ccgccgacta ctgggatgct gccgacctgt atgaacgcgc
      301 caatgggcgg ctgttcaagg aggtcgaatt tgccctgccg gtcgagctga ccctcgacca
      361 gcagaaggcg ctggcgtccg agttcgccca gcacctgacc ggtgccgagc gcctgccgta
      421 tacgctggcc atccatgccg gtggcggcga gaacccgcac tgccacctga tgatctccga
      481 gcggatcaat gacggcatcg agcggcccgc cgctcagtgg ttcaagcggt acaacggcaa
      541 gaccccggag aagggcgggg cacagaagac cgaagcgctc aagcccaagg catggcttga
      601 gcagacccgc gaggcatggg ccgaccatgc caaccgggca ttagagcggg ctggccacga
      661 cgcccgcatt gaccacagaa cacttgaggc gcagggcatc gagcgcctgc ccggtgttca
      721 cctggggccg aacgtggtgg agatggaagg ccggggcatc cgcaccgacc gggcagacgt
      781 ggccctgaac atcgacaccg ccaacgccca gatcatcgac ttacaggaat accgggaggc
      841 aatagaccat gaacgcaatc gacagagtga agaaatccag aggcatcaac gagttagcgg
      901 agcagatcga accgctggcc cagagcatgg cgacactggc cgacgaagcc cggcaggtca
      961 tgagccagac ccagcaggcc agcgaggcgc aggcggcgga gtggctgaaa gcccagcgcc
     1021 agacaggggc ggcatgggtg gagctggcca aagagttgcg ggaggtagcc gccgaggtga
     1081 gcagcgccgc gcagagcgcc cggagcgcgt cgcgggggtg gcactggaag ctatggctaa
     1141 ccgtgatgct ggcttccatg atgcctacgg tggtgctgct gatcgcatcg ttgctcttgc
     1201 tcgacctgac gccactgaca accgaggacg gctcgatctg gctgcgcttg gtggcccgat
     1261 gaagaacgac aggactttgc aggccatagg ccgacagctc aaggccatgg gctgtgagcg
     1321 cttcgatatc ggcgtcaggg acgccaccac cggccagatg atgaaccggg aatggtcagc
     1381 cgccgaagtg ctccagaaca cgccatggct caagcggatg aatgcccagg gcaatgacgt
     1441 gtatatcagg cccgccgagc aggagcggca tggtctggtg ctggtggacg acctcagcga
     1501 gtttgacctg gatgacatga aagccgaggg ccgggagcct gccctggtag tggaaaccag
     1561 cccgaagaac tatcaggcat gggtcaaggt ggccgacgcc gcaggcggtg aacttcgggg
     1621 gcagattgcc cggacgctgg ccagcgagta cgacgccgac ccggccagcg ccgacagccg
     1681 ccactatggc cgcttggcgg gcttcaccaa ccgcaaggac aagcacacca cccgcgccgg
     1741 ttatcagccg tgggtgctgc tgcgtgaatc caagggcaag accgccaccg ctggcccggc
     1801 gctggtgcag caggctggcc agcagatcga gcaggcccag cggcagcagg agaaggcccg
     1861 caggctggcc agcctcgaac tgcccgagcg gcagcttagc cgccaccggc gcacggcgct
     1921 ggacgagtac cgcagcgaga tggccgggct ggtcaagcgc ttcggtgatg acctcagcaa
     1981 gtgcgacttt atcgccgcgc agaagctggc cagccggggc cgcagtgccg aggaaatcgg
     2041 caaggccatg gccgaggcca gcccagcgct ggcagagcgc aagcccggcc acgaagcgga
     2101 ttacatcgag cgcaccgtca gcaaggtcat gggtctgccc agcgtccagc ttgcgcgggc
     2161 cgagctggca cgggcaccgg caccccgcca gcgaggcatg gacaggggcg ggccagattt
     2221 cagcatgtag tgcttgcgtt ggtactcacg cctgttatac tatgagtact cacgcacaga
     2281 agggggtttt atggaatacg aaaaaagcgc ttcagggtcg gtctacctga tcaaaagtga
     2341 caagggctat tggttgcccg gtggctttgg ttatacgtca aacaaggccg aggctggccg
     2401 cttttcagtc gctgatatgg ccagccttaa ccttgacggc tgcaccttgt ccttgttccg
     2461 cgaagacaag cctttcggcc ccggcaagtt tctcggtgac tgatatgaaa gaccaaaagg
     2521 acaagcagac cggcgacctg ctggccagcc ctgacgctgt acgccaagcg cgatatgccg
     2581 agcgcatgaa ggccaaaggg atgcgtcagc gcaagttctg gctgaccgac gacgaatacg
     2641 aggcgctgcg cgagtgcctg gaagaactca gagcggcgca gggcgggggt agtgaccccg
     2701 ccagcgccta accaccaact gcctgcaaag gaggcaatca atggctaccc ataagcctat
     2761 caatattctg gaggcgttcg cagcagcgcc gccaccgctg gactacgttt tgcccaacat
     2821 ggtggccggt acggtcgggg cgctggtgtc gcccggtggt gccggtaaat ccatgctggc
     2881 cctgcaactg gccgcacaga ttgcaggcgg gccggatctg ctggaggtgg gcgaactgcc
     2941 caccggcccg gtgatctacc tgcccgccga agacccgccc accgccattc atcaccgcct
     3001 gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa gccgtggctg acggcctgct
     3061 gatccagccg ctgatcggca gcctgcccaa catcatggcc ccggagtggt tcgacggcct
     3121 caagcgcgcc gccgagggcc gccgcctgat ggtgctggac acgctgcgcc ggttccacat
     3181 cgaggaagaa aacgccagcg gccccatggc ccaggtcatc ggtcgcatgg aggccatcgc
     3241 cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc agcaagggcg cggccatgat
     3301 gggcgcaggc gaccagcagc aggccagccg gggcagctcg gtactggtcg ataacatccg
     3361 ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc gaggaatggg gtgtggacga
     3421 cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag gccaactatg gcgcaccgtt
     3481 cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc aagcccgccg tgctggagag
     3541 gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa gaacaagcac agcctcagcc
     3601 acgtccggca cgacccggcg cactgtctgg cccccggcct gttccgtgcc ctcaagcggg
     3661 gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta cggcgacggc aagcggatcg
     3721 agttcagcgg cccggagccg ctgggcgctg atgatctgcg catcctgcaa gggctggtgg
     3781 ccatggctgg gcctaatggc ctagtgcttg gcccggaacc caagaccgaa ggcggacggc
     3841 agctccggct gttcctggaa cccaagtggg aggccgtcac cgctgatgcc atggtggtca
     3901 aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga ggtcgatagt ggtggggcgc
     3961 tcaagcacat acaggactgc atcgagcgcc tttggaaggt atccatcatc gcccagaatg
     4021 gccgcaagcg gcaggggttt cggctgctgt cggagtacgc cagcgacgag gcggacgggc
     4081 gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt catgggtggc ggccagcatg
     4141 tgcgcatcag catggacgag gtgcgggcgc tggacagcga aaccgcccgc ctgctgcacc
     4201 agcggctgtg tggctggatc gaccccggca aaaccggcaa ggcttccata gataccttgt
     4261 gcggctatgt ctggccgtca gaggccagtg gttcgaccat gcgcaagcgc cgccagcggg
     4321 tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac ggtaaccgag ttcgcggcgg
     4381 gcaagtacga catcacccgg cccaaggcgg caggctgacc cccccactct attgtaaaca
     4441 agacattttt atcttttata ttcaatggct tattttcctg ctaattggta ataccatgaa
     4501 aaataccatg ctcagaaaag gcttaacaat attttgaaaa attgcctact gagcgctgcc
     4561 gcacagctcc ataggccgct ttcctggctt tgcttccaga tgtatgctct tctgctcctg
     4621 cagctaatgg atcaccgcaa acaggttact cgcctgggga ttccctttcg acccgagcat
     4681 ccgtataata ctcatgcccg atcttcattc gagcaacgga gttctctgtg tcttttagca
     4741 cctcttcatc gcggcctgcg caagtatcgg tcgctcgttc ctgggcagtc cttgtttgtc
     4801 tgtccatcct gcaattcctc atcgccgtcg acgtcaccgt ggtcaacgtg gcgctgccct
     4861 ctatcggcaa cgatctgggc gttgatgcgc gcggcctgac atgggtcgtc gttggttaca
     4921 ccatcacagg cggtggtctg ttgatgcttg gtggacgtct gggagacgtc ttcggccgcc
     4981 gccgccttct gctcatcgga accgcgatct tcggagcggc ctcgctgatg gcgggcctcg
     5041 cggagacctt tccgctactc gtcggtgcaa ggttgttgca gggtgtaggg gaagccctcg
     5101 ccctgcccgc tgcgatggcg accatcgtcc tgttgtttcc cgaagggcgg gcgcgcaccc
     5161 gcgccctgag cgtctgggct gcggttgcga gctgcggtct tgtgctgggg ttcgtgctct
     5221 cgggaatcat caccgagtat ctcggttggc gatggatctt cctcatcgcc gttccgttcg
     5281 tcctgttcgt gctgatcgct tctgtcgccc tgctacctcg agaccgatca caggagcgcg
     5341 caccgttgga tattctcgga tcgattctgc tgacgagcgc accgctgctg ttcgctttcg
     5401 gagtggtgga ggccggggac agcaatgggg atgtgccctg gtcatcaatc gctgccctgg
     5461 tgggagcggc agccgccgct gcattgttcg tcaaggtgga acgccgagcc gcaaacccac
     5521 tggttccgct gaccttcttc cgcaaccgga cacgagtgcg agcaaatctt gcgactgcac
     5581 tgttgagcgc ggcgctctca acgtcctttc tgctgttcac gttctatcta caggatggac
     5641 tcggtctctc tccgctggaa tccggattgc tgctcctgcc acttgcggtg gcactagtcg
     5701 tcgccgtgac cgtcgtacct cgcctcttgg ggcgctgggg agcgcgtgct tgcatcgtcg
     5761 tgggactgag ttccgcaggg gcaggcatga tcgtgatcgc ggcagccgcg cagctcgaag
     5821 cccccgcttg gctactaacc cccgcgatgc tcttcatcgc cgcaggaatg ggtttcggcc
     5881 tcgttggatt gcagtacgcg gccgtgaccg aggtcaccga cgatgatgcc ggagtcgcat
     5941 ccggcgtaca gcgagccgca gaccagctcg gtggatccgc aggtgtcacg gtctacgtgg
     6001 gcctgggctt cgcgtcggcc cttcacggtg tatcacccta tgtgctaagt agcggcctcg
     6061 cgatcatcgg cctctgcctt gcgaccgcca ttgtccaacg aatcgtaatc gtgacgcagg
     6121 aacaacagac gaggacatga gcgtgccgtc gtaacccgat cccgaagttc ccgatgaggt
     6181 gctccgtggc gacgcccggc gactcgtcag tcagccccga ccggtggtcg cggggcgcgc
     6241 cctttgttgc gcgcaccgcg accattcacc gccctctcgg tccggtggcg tatgactgcg
     6301 tcaaggtgat cgtggtgcgt gacggctctg cgttcttgtt cagcgagttt ggacagcaga
     6361 cggtgaaacc cggagacgtg atcttgctcg gtgcgaacgt actggcagga tcggagccag
     6421 agggccacat caccgtcacc acgatctatc tcgacaccga ctacatgctc gaccaggtgc
     6481 gctggcagta cgccgcctac ctcgaagatc gactcgacgc gcaagagttc taatataccc
     6541 ccttgccggg acaggacatt ctccgtgccg atgtgtacaa ccaggtcttt accctgcacg
     6601 gcacggtgat gatgttttta tttgcgattc cggtgctcga aggtctggcg atgtatctca
     6661 tacccaaaat gctcggcacc cgggatctgg cgtttcccgc cactgcacga ggcgtaactg
     6721 ccgcgacgct gaactgtcgg taacgatctg ctcaacgcca gccagtgccc gtcactcccg
     6781 tactaactgt cacgcccccc tgcaataact gtcacgcccc cctgcaataa ctgtcacgcc
     6841 cccctgcaat aactgtcacg aacccctgca ataactgtca cgcccccaaa cctgcaaacc
     6901 cagcaggggc gggggctggc ggggtgttgg aaaaatccat ccatgattat ctaagaataa
     6961 tccactaggc gcggttatca gcgcccttgt ggggcgctgc tgcccttgcc caatatgccc
     7021 ggccagaggc cggatagctg gtctattcgc tgcgctaggc tacacaccgc cccaccgctg
     7081 cgcggcaggg ggaaaggcgg gcaaagcccg ctaaacccca caccaaaccc cgcagaaata
     7141 cgctggagcg cttttagccg ctttagcggc ctttccccct acccgaaggg tgggggcgcg
     7201 tgtgcagccc cgcagggcct gtctcggtcg atcattcagc ccggctcatc cttctggcgt
     7261 ggcggcagac cgaacaaggc gcggtcgtgg tcgcgttcaa ggtacgcatc cattgccgcc
     7321 atgagccgat cctccggcca ctcgctgctg ttcaccttgg ccaaaatcat ggcccccacc
     7381 agcaccttgc gccttgtttc gttcttgcgc tcttgctgct gttcccttgc ccgcacccgc
     7441 tgaatttcgg cattgattcg cgctcgttgt tcttcgagct tggccagccg atccgccgcc
     7501 ttgttgctcc ccttaaccat cttgacaccc cattgttaat gtgctgtctc gtaggctatc
     7561 atggaggcac agcggcggca atcccgaccc tactttgtag gggagggcgc acttaccg
//
