LOCUS       NZ_AP023223             2101 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli strain M505 plasmid pM505-c, complete sequence.
ACCESSION   NZ_AP023223
VERSION     NZ_AP023223.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00099774
            Sequence Read Archive: DRR111587, DRR232346
            Assembly: GCF_013374235.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Sugawara,Y., Akeda,Y., Hagiya,H., Sakamoto,N., Takeuchi,D.,
            Shanmugakani,R.K., Motooka,D., Nishi,I., Zin,K.N., Aye,M.M.,
            Myint,T., Tomono,K. and Hamada,S.
  TITLE     Spreading Patterns of NDM-Producing Enterobacteriaceae in Clinical
            and Environmental Settings in Yangon, Myanmar
  JOURNAL   Antimicrob Agents Chemother 63 (3), e01924-18 (2019)
   PUBMED   30530602
  REMARK    DOI:10.1128/AAC.01924-18 Publication Status: Online-Only
REFERENCE   2
  AUTHORS   Sugawara,Y., Hagiya,H., Akeda,Y., Takeuchi,D., Sakamoto,N.,
            Matsumoto,Y., Motooka,D., Nishi,I., Tomono,K. and Hamada,S.
  TITLE     Community spread and acquisition of clinically relevant
            carbapenemase-producing Escherichia coli in healthy Japanese
            residents of Yangon, Myanmar, 2018
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 2101)
  AUTHORS   Sugawara,Y., Akeda,Y., Sakamoto,N., Takeuchi,D. and Hamada,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2020) Contact:Yo Sugawara Osaka University,
            Research Institute for Microbial Diseases; 3-1 Yamadaoka, Suita,
            Osaka 565-0871, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.5
            Genome Coverage                   :: 169x
            Sequencing Technology             :: MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 03:43:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,950
            Genes (coding)                    :: 4,693
            CDSs (with protein)               :: 4,693
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 92 of 257
            Pseudo Genes (incomplete)         :: 175 of 257
            Pseudo Genes (internal stop)      :: 51 of 257
            Pseudo Genes (multiple problems)  :: 58 of 257
            Pseudo Genes (short protein)      :: 4 of 257
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023223.2.
            On Apr 28, 2021 this sequence version replaced NZ_AP023223.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="M505"
                     /isolation_source="human blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pM505-c"
                     /geo_loc_name="Myanmar:Yangon"
                     /lat_lon="16.7788 N 96.1490 E"
                     /collection_date="2016-06-01"
     gene            join(1831..2101,1..695)
                     /locus_tag="EPG46_RS24970"
     CDS             join(1831..2101,1..695)
                     /locus_tag="EPG46_RS24970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            699..965
                     /locus_tag="EPG46_RS24975"
     CDS             699..965
                     /locus_tag="EPG46_RS24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          124..355
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 aagcccgttt ttatcaggct ctaccgaaaa tcgttgtgga ttacccgtct tcccgatggt
       61 tgtttctgac gttaactgtc aggaactgcg agataggtga acttggaaca gtccttacag
      121 caatgaatgc ggcgtttaag cgaatggaaa agcgaaagga gctatcacct gttcaggggt
      181 ggatcagggc tacggaggtg acgcgaggta aggatggcag cgcacatccg cattttcact
      241 gtctgctgat ggtgcaacct tcttggttta aagggaagaa ctacgttaag cacgaacgtt
      301 gggtagaact ctggcgcgat tgcttgcggg tgaactatga gccgaatatc gatattcggg
      361 cagtaaaaac taagacaggt gaggttgtgg ccaacgttgc cgagcaactg caaagcgcgg
      421 ttgctgaaac gctgaaatac tccgttaaac cggaagatat ggcaaacgat cctgagtggt
      481 ttcttgagct gacgcggcag cttcacaagc gccgttttat ctcgaccggt ggggcgctaa
      541 aaaacgtcct ccagttggat cgagaaacca atgaggatct tgtcattgcc gacgatgtag
      601 gggatggcac tgatgacggg aagcggacgg cgtttgtctg ggattcaggt aaacggcgtt
      661 acaaacgcgc ccctgagaag gataaatcgg attaacgtat gaatattaat attgaatacc
      721 tgaatggaaa taagactatt ggtttatttt ttttaagaag tgaagcggtg attcctgaca
      781 ggtttaaaaa ccttattttg cttattgatg gattaagttt tggcacattt ggttttcatc
      841 cgcacgaagg ttttgaggat gaattaattt tatatattca gaaaacaaac gagagggtaa
      901 aaactctttt tgtgaaaata gatttaaacg gtatcaagtt tggattttta agaacgcatt
      961 cttagttctg gaaaagagcc agcggcaggc tgaggtgata ggtacgagat tgcatgcaat
     1021 ctctagtgct ctgtctatcc tgcattatcc tcagcattat cctcagcatt atcctcagcc
     1081 ttgccaactc gacaccaatg caggatagac aatccgatgt caaatgttaa cactctgcga
     1141 gtggtacatt ttccccggat tatcgtcctg agcctgccgc tggctctctt tctaccgcct
     1201 cgctttgctc gttgctcaac gcctcacaga cacggattaa aatccgcatc cgttcaccgt
     1261 tttttaaagt ccgttaaaag catgatgcca tctccgagag ttaatctcgt caaatgctaa
     1321 atcgtggggg tcccctttgg ggttccgatt tagtgattga cgacaccacc gattaaaaaa
     1381 cttatgcggg gtggatggtt tcacgaagtg aggccatcca cctgtaagac agggttttgt
     1441 ttttattccc tgttttggtg atcgggtgtg tggaaaaggt tggggtaagc cgttcggggg
     1501 tgcttgtttt ggggggttaa aattgtggtt attttttgcg caattctcgc gcgtgatcct
     1561 tgtatttata cttaagggat aaatggcgga tatgaaatag tggtttagcc cagtaatgac
     1621 gaggctttga gtgggttttg acaggtcaaa gaaaatggag cagaattgag gcgtttttaa
     1681 tcggcgttgg ggagtgcgtc aacactcccc aacatttcga atgtgtcacc tcagcggcaa
     1741 actctggtga catgtactgg ctcgcaatgc acaggtacgt gatgaatata ccacatcaaa
     1801 tcacagcctg cccagatcgg agcaggctta atgtcagaag ataaattcct ttcggactac
     1861 agcccccgtg atgcagtttg ggatacccag cgcacgctta ccgattctgt cgggggtatc
     1921 taccagactg ctgctgaatt cgagcgctat gcactccgta tggcctcctg tagcggtttg
     1981 ttacgttttg gttggtctac catcatggaa accggagaaa cgcgcctacg gcttcgtagt
     2041 gcgcaatttt gccgtgtccg tcattgccct gtctgccagt ggagaagaac cctcatgtgg
     2101 c
//
