LOCUS       NZ_AP023211             1962 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain TUM18781 plasmid pMTY18781-6, complete
            sequence.
ACCESSION   NZ_AP023211
VERSION     NZ_AP023211.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00172788
            Assembly: GCF_013340825.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Ito,Y., Aoki,K., Nakayama,N., Ohtsuka,M., Ota,M., Kaneko,N.,
            Yoshida,M., Ishii,Y., Tateda,K. and Matsuse,H.
  TITLE     Whole-genome sequencing of blaNDM-5 positive Escherichia coli
            isolated from a Japanese patient with no history of travel abroad
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Ito,Y., Aoki,K., Nakayama,N., Ohtsuka,M., Ota,M., Kaneko,N.,
            Yoshida,M., Ishii,Y., Tateda,K. and Matsuse,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2020) Contact:Yukitaka Ito Toho University,
            Clinical Microbiology Laboratory; 2-22-36 ohashi, Meguro-ku
            153-8515, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler Assembly v. 0.4.4
            Genome Coverage                   :: 186x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 02:42:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,639
            CDSs (total)                      :: 4,523
            Genes (coding)                    :: 4,414
            CDSs (with protein)               :: 4,414
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 46 of 109
            Pseudo Genes (incomplete)         :: 58 of 109
            Pseudo Genes (internal stop)      :: 24 of 109
            Pseudo Genes (multiple problems)  :: 21 of 109
            Pseudo Genes (short protein)      :: 2 of 109
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023211.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM18781"
                     /isolation_source="human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMTY18781-6"
                     /geo_loc_name="Japan:Tokyo, Meguro-ku"
                     /collection_date="2018-05-01"
     gene            complement(375..536)
                     /locus_tag="HUV20_RS23000"
                     /old_locus_tag="TUM18781_44190"
     CDS             complement(375..536)
                     /locus_tag="HUV20_RS23000"
                     /old_locus_tag="TUM18781_44190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045343414.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSLE
                     VMRLLRLN"
                     /name="hypothetical protein"
     Typing          complement(420..917)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.589"
                     /coverage="82.0"
                     /evalue="0.0"
ORIGIN
        1 gagccggtat tcctttctat caatatgaag gaataaacgt atggccgaat tgcttacggc
       61 gtttattaaa gcaccactta taagccaggt gctgaccatc attctgtgtc tgatctttgt
      121 agtacacagt cttttaaatt gacgtaccac ccataaaaaa agccacttat gtggcttttt
      181 ttatgaatcg agagataggt atctgtcgaa tatcacagat gatcttcttg agatcctgtc
      241 cgttgcgtcg taatctcttg ctctggaaac gaaaaaaccg ccctgcaagg cggtttttcg
      301 aaggttctca gagctaccaa ctctttgaac cgcggtaact ggcttggagg agcgcagtaa
      361 ccaaatctgt cctttcagtt tagccttaac aggcgcataa cttcaagact aactcctcta
      421 aatccgttac caatggctac cgccactggc gatttgtcgt gtctttccgg gttggactca
      481 agttgatagt taccggaacg ggcgtggtag tcggactgaa cggggggttc gtgcatacag
      541 tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagataaacg
      601 cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag
      661 ggagccacca gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccaccactga
      721 tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttc
      781 gccgtggcct tcttgcttcc ctgttaagct tcttcctgga agatgccagg aaatttccgc
      841 cccgccagta agccggtacc gctcgccgca gccgaacgac cgagcggagc gagtcagtga
      901 gcgaggaagc ggaatatctc cgggcttatc tttggcaccg tacgcgccat agctgcattt
      961 tttaatacga tgcaggatag ggctaacgcc gcaaagtgac gtcacctgac gttcctggat
     1021 tacaaaggtt aaagcagcgg acaggaaatg ttttgtgccc acagctatgc tggcttattc
     1081 acaaaacagc gggacaagat gtgtttttgg agtaccgccc atactccaaa acgctatgac
     1141 catgctctgc ccgataccct tagcaatcag tgtttgcaat ctgtcccgaa cctgtttagg
     1201 aagtgtttgc aatctgtccc gaacctgttt aggaagtgtt tgcaatctgt cccgaacctg
     1261 tttaggaagt gtttgcaacc tgtcccgaat ctgtttgctc agtgtttata gcctgttttc
     1321 tcactgattt actctgcatg tccaaggcac gttagcgatg tatacatcgc cgcgccgcaa
     1381 gggtgcccct tccgaatccc gttcatcact gcgtgctgat taatccacgt taaatccgac
     1441 ccccttagca ataaatccag ccatgcctga cggcctggcc aacgcctcac ggccgcacag
     1501 taaactgcgc acccgtgagg cgttattcgt gaggttaagt ggtgcttttg gacgtcctgc
     1561 cggattttag ccgctaaaat cccggccatg catgtgtgtg catagccatg cagactcgta
     1621 gaaatcatca ggcgcgtttt ttcacgatat aggcacaggg aagtgccgga aacagtcatc
     1681 ccgccgcaga aatgcgctga gggcgtctga gggggtgcgg gcaacggatg ttatggtaaa
     1741 tctagctgct agggcgattc gcacgccaca ttttcagatg tgatatcatt acgtcctagg
     1801 ggtgaaaagg gcaaaaaaaa aagaacccgg aatggttctt ttttttgcct caatcagcga
     1861 gtgagaatcg ctgattgctg cccgaaaaca tgtaccgaag ctcacgtcgt cgttaacttt
     1921 tgacaaagta atgttacccc ttgggtgaat ctacgtcaac gt
//
