LOCUS       NZ_AP023203             8136 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain TUM18780 plasmid pMTY18780-6, complete
            sequence.
ACCESSION   NZ_AP023203
VERSION     NZ_AP023203.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00172787
            Assembly: GCF_013340805.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Ito,Y., Aoki,K., Nakayama,N., Ohtsuka,M., Ota,M., Kaneko,N.,
            Yoshida,M., Ishii,Y., Tateda,K. and Matsuse,H.
  TITLE     Whole-genome sequencing of blaNDM-5 positive Escherichia coli
            isolated from a Japanese patient with no history of travel abroad
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8136)
  AUTHORS   Ito,Y., Aoki,K., Nakayama,N., Ohtsuka,M., Ota,M., Kaneko,N.,
            Yoshida,M., Ishii,Y., Tateda,K. and Matsuse,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2020) Contact:Yukitaka Ito Toho University,
            Clinical Microbiology Laboratory; 2-22-36 ohashi, Meguro-ku
            153-8515, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler Assembly v. 0.4.4
            Genome Coverage                   :: 173x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 02:30:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,360
            CDSs (total)                      :: 5,240
            Genes (coding)                    :: 5,050
            CDSs (with protein)               :: 5,050
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 63 of 190
            Pseudo Genes (incomplete)         :: 131 of 190
            Pseudo Genes (internal stop)      :: 32 of 190
            Pseudo Genes (multiple problems)  :: 31 of 190
            Pseudo Genes (short protein)      :: 2 of 190
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023203.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM18780"
                     /isolation_source="human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMTY18780-6"
                     /geo_loc_name="Japan:Tokyo, Meguro-ku"
                     /collection_date="2018-05-01"
     gene            join(8014..8136,1..69)
                     /locus_tag="HUV22_RS26475"
     CDS             join(8014..8136,1..69)
                     /locus_tag="HUV22_RS26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            926..1841
                     /locus_tag="HUV22_RS26485"
                     /old_locus_tag="TUM18780_51350"
                     /pseudo=""
     CDS             926..1841
                     /locus_tag="HUV22_RS26485"
                     /old_locus_tag="TUM18780_51350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004088970.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1854..2792
                     /locus_tag="HUV22_RS26490"
                     /old_locus_tag="TUM18780_51360"
     CDS             1854..2792
                     /locus_tag="HUV22_RS26490"
                     /old_locus_tag="TUM18780_51360"
                     /inference="COORDINATES: protein motif:HMM:NF017515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="WP_174402719.1"
                     /translation="MRYISGLKTLAKDFYHYNFSGMNELFSYDDTHEDNIEIFMKDNSF
                     LEIPKVIWMYWDDASIPPYISLMIDKIRNDNKDHDLVVLNKNNLVSYIEDLKFSSDKYI
                     PIANKSDIIRLELLKRYGGIWIDSSVILTKPISWVHDINKDKKVDLIGYYRDVSTKDRM
                     YPIVESWFLCSQKNNLFINEWLDVLSPLKEMGAKEYFNMIKSRPDYEVILQGIHKPDYL
                     LVYLAQQIAMRKINFNLFSKRSESSAFLYQENESWNSSAIARDLCIKKSPITLPPVIKL
                     TNDNRRGIEKLIKLKLVKKDSIIGKLMSGAL"
                     /name="capsular polysaccharide synthesis protein"
     gene            2857..3554
                     /locus_tag="HUV22_RS26495"
     CDS             join(2857..3106,3106..3554)
                     /locus_tag="HUV22_RS26495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_250908635.1"
                     /translation="MASVSISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTILRHFKKLRPQSVNSRIQPGSNVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVVHVFGERTMATLERLPGLLSAFEVVVW
                     MRDGWPLYEPRLKGELHVISKRYTQRIERHNLNLRQHLSRLGRKSLSFSKSVELHDKVI
                     GHYLNIKHDL"
                     /name="IS1 family transposase"
     gene            4288..4519
                     /locus_tag="HUV22_RS26940"
                     /pseudo=""
     CDS             4288..4519
                     /locus_tag="HUV22_RS26940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332449.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            5078..6301
                     /locus_tag="HUV22_RS26500"
                     /old_locus_tag="TUM18780_51390"
     CDS             5078..6301
                     /locus_tag="HUV22_RS26500"
                     /old_locus_tag="TUM18780_51390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013512451.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="WP_250908633.1"
                     /translation="MMAEKLVSIIGLVFVNSYMAKYIGPTDFGKITFATTIFTFVQAMA
                     WFGGQNILFKRMSEKRKSGIALALHTQKQRRVIFALSSLIALFYLFFFTDTLTFVFGVA
                     NCIATYYIVMDIFAVYNNTQLISRINALTNGAGLCIAYIFRFLISYLELPVYTFSIPII
                     LIPAIPYVIRCIYFYNTVEDTSVLKSKTKKYNKYLISAGGSLLLSTLSIVVYTQVSNIF
                     LAKVTSYSNLGIYNVALTLGSAYSFISVALITSFFSKIYQEKDFEKMSRLLTSINRIVI
                     VITIFILFGFYFLGPYIISMLYGSAYKMAVELVPIIILATMFSSLGAICYRFIIKYSGY
                     KYLSYKMMLTGLLSIPFSYVMINRYDVFGAAYCFLVVEVLSCTLLNYFFRSGMILRIHL
                     NVIGLMRR"
                     /name="oligosaccharide flippase family protein"
     gene            6394..7091
                     /locus_tag="HUV22_RS26505"
     CDS             join(6394..6643,6643..7091)
                     /locus_tag="HUV22_RS26505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_250908635.1"
                     /translation="MASVSISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTILRHFKKLRPQSVNSRIQPGSNVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVVHVFGERTMATLERLPGLLSAFEVVVW
                     MRDGWPLYEPRLKGELHVISKRYTQRIERHNLNLRQHLSRLGRKSLSFSKSVELHDKVI
                     GHYLNIKHDL"
                     /name="IS1 family transposase"
     Typing          complement(3949..4430)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.775"
                     /coverage="93.0"
                     /evalue="4.5400000000000006e-173"
     Typing          complement(7252..7777)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.43"
                     /coverage="99.0"
                     /evalue="1.3e-168"
ORIGIN
        1 catgaggatg cggaacgcct ttaccgaagc ctgtcagcgc gcctgaatga gcagcaggac
       61 ggtgcgtaat cgctccgttg tgggtattac cggagtaata ccggcatcgc cttaattttc
      121 attgtcttgg cgcttaatat tattaccccg gtaataccca gaatccctgt attttttctt
      181 ttttatttat ttttcatgaa gttgcgatgt acatcgcagc tgcgttaggt gtatgactat
      241 ttaagggtta aaaaatcatc atttttggta gggcgctctc cgtaggttaa gggttacttg
      301 gctaaaaaac gtcctgttct ttgatggtca tgcttgcatg accatctgag caaccaaaaa
      361 ctacagataa actacaaagt aactaccgga ttacctcaga tccgttccct gaaccattcg
      421 tacatccgcc ccctcttcct gggcctcctg cacgtgctga aaatggtttt tccggctgtg
      481 ccggcaacgc ctcacggcca cgcaataaat tgcgtccccg ttcaccgttt tcggatggga
      541 agaacgccgg aaacaggact taccccggct gaatggcgtg aatttccgcg ctatgcactt
      601 gcgcgcatac tcatgcatgc cgtaaaaaca gagcctgcgc gtttctggcg ggttttcggg
      661 tggtttgttg cctgttttgc cggtttcccg tcagaaacgc gctgagggcg cctgaggcgg
      721 tgcgggcaac ggatgttatg gtaaatctcg gcgggatcga cagatgtggt cagagggtgt
      781 aactttaagg tcagaaaaaa caaagccccg gaaatcatgt gctatcttgg cggatctcat
      841 gataaccagg gccaggtcga aaccttgaaa ggatattagc acatgaaccg tgccagacaa
      901 cagcccgcca taagcgcagg gcacattgcg taacgccgca ctcggacttt ttaacgaccg
      961 cttaccgcac aagccgtact tatcggacga tctgcacttc ggtgtacgca ttgcagggaa
     1021 agagcgcgct atcctggcga aatacatcca gttcaaccag ccttacgcca tgttctggct
     1081 gggatttgac gtcgatcgcg tcggcgcggc tatcgactgg agcgaccgta acgcccccgc
     1141 acctacgttg accattacca atcccgaaaa tggtcacgcg cacctgctgt acgcgctgga
     1201 aacctcgatc cgcaccgccc cggacggaaa aatgaagccg ctgaagtacg ctgctgtcgt
     1261 agaaaacgcg ctgcgtaaaa aattaggcgc tgacacaggc tattctggcc tgatttgcaa
     1321 gaacccgaac cacagtcact ggaaaatcgc cgtgtggcag ccgcagcttt acacgctgga
     1381 ctggttagcc gacttactcg acctgaccgc cgcgaacgac aaagaaatcg ttgctgacta
     1441 cggattagtc cgtaactgca ccctgtttga taaaacccgt aagtgggcat atcgcgctat
     1501 ccgccagggc tggcctgaat atgaccagtg gctgcaagcc tgctatgaac gtgccagtgc
     1561 ctataatctg caattttcag ctcctctgga tgaaaatgag gtgaatggga tcgctaaaag
     1621 cattgctaag tggactcacg ggaaatttac ggcagaatca ttttatgaat ttgtaaaaag
     1681 tacacatatg tcaaaaattc aatctgaaag aggaaggaag gggggggatt ggtggtgtaa
     1741 taagccgtgg aggagggagg cctaaaagaa aaagtagtcc atggattgat ttgggtatta
     1801 gtcgtagcac ttggtatcga aagtatttta aaattaattg aaatagggtg tgtatgagat
     1861 acattagtgg gttaaaaaca cttgcaaaag atttttatca ttataatttt tccggaatga
     1921 atgagttgtt tagctatgat gatacgcatg aagataacat agaaatattt atgaaagata
     1981 attcttttct ggaaataccg aaggtaatct ggatgtactg ggatgacgct agtattccgc
     2041 cttacataag tctgatgata gataaaataa ggaatgacaa caaagatcat gatttagttg
     2101 ttttgaataa aaataattta gtcagttaca ttgaagattt gaaattttct tctgataaat
     2161 acattcctat agcaaacaag tcagacataa taagacttga attattaaag cgttacggag
     2221 gaatatggat tgactcttct gttatcctga caaagcccat atcatgggtt catgatatta
     2281 ataaagataa aaaagtcgat cttattggtt attataggga tgtctcaacg aaagatagaa
     2341 tgtatcctat tgttgagagc tggtttttat gctcacagaa aaataatttg ttcattaatg
     2401 aatggcttga tgttctttca ccattaaaag aaatgggtgc aaaggaatac tttaatatga
     2461 taaaatccag gcccgactat gaagttattc tccaaggcat tcacaagcct gactatttac
     2521 ttgtttacct cgcacagcaa attgcaatga gaaaaattaa ctttaattta ttttctaaaa
     2581 gaagcgagtc cagtgcattc ttatatcaag agaatgagag ttggaactct tcagctatag
     2641 caagggatct ttgcattaaa aaatctccga taacattacc accagtaata aaactaacca
     2701 atgataatag gcgcggaatt gaaaagctca taaaattaaa attagtaaag aaagatagca
     2761 tcattggaaa attaatgtca ggagcactct aacgagaagg cggtaatggt gccaacttac
     2821 tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc tatcagttgt
     2881 ccctcctgtt cagctactga aggcgtggtg cgtaacggta aaagtactgc cggacatcag
     2941 cgctatctct gctctcactg ccgtaaaaca tggcagctac agttcactta caccgcctct
     3001 cagcccggta cacaccagaa aattattgat atggccatga atggcgtcgg atgtcgcgcc
     3061 agtgcacgca ttatgggcgt tggcctcaac acgattttac gacacttaaa aaactcaggc
     3121 cgcagtcggt aaactcacgc atacaaccgg gcagtaacgt cattgtttgc gcggaaatgg
     3181 acgaacagtg gggttacgtc ggcgctaaat cacgccagcg ctggttgttt tacgcgtatg
     3241 acaggatacg gaggacagtt gtggtgcacg tattcggtga acgcacgatg gccacgctgg
     3301 agcgtcttcc gggcctgctg tcggcctttg aggtcgtggt atggatgagg gatggctggc
     3361 cgctttatga accacgcctg aagggagaac tgcacgttat cagcaagcga tatacgcagc
     3421 gtattgagcg gcataacctg aatctgaggc agcatctgtc aaggctgggc aggaagtcac
     3481 tgtcgttctc aaaatcggtg gagctgcatg acaaagtcat cgggcattat ctgaacataa
     3541 aacacgatct gtaagttgga gtcattacct acagtttcat atgtgggcgt ttatgtaaca
     3601 tatgtcgctg ggccaactct tctggtttct gggtttttat cgtggattac aaggccgtaa
     3661 tctgataatg cccctaatcg gtcagatctc taggatagta gcaatacagc aaggaacttc
     3721 ttgagatcct ttctgttgcg tcgtaatctc atgtcctgca aacaaaaaaa ccaccgctac
     3781 caacggtggt tcgtttgccg gaatcaagag ctaccaactc ttttttccga aggtaactgg
     3841 ctttcacgga gcgcagatac caaaacagtc cttccagtgt agccgcagtc aggccaccac
     3901 ttcaagaact ctgtagaagc actctactac tctgctccgt aaatcctgtt accagtggct
     3961 gctgccagtg gcgttttgtc gtgcctctcc gggttggact caagacgata gttaccggat
     4021 aaggcgcagc ggtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact
     4081 gcctacccgg aactgagtgt caggcgtgga atgagataaa cgcggccata acagcggaat
     4141 gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccat cagggggaaa
     4201 cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc
     4261 gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc aacgccgcat cctttctgag
     4321 acctctttat tcctgtgtca tagctttatg ctcttatccg tgagcttcgt ccgctcgccg
     4381 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaatatc acctgaacca
     4441 gcatcttctc ctgacgccgg aaagcctcat ttcactcctg ccacatgaag cactttggca
     4501 aatcaccact tcggtttaaa atattaagaa aacgttacgc catcataagc aagggtgaaa
     4561 tcggtccttc ctgaagttat cgtgtttatt gttaagttac ttaatgattt gacctcaacc
     4621 caaatgttaa agatagtcgt tagcgttagt gcagtggata ggtagctgaa gagcccaggg
     4681 cggtagcgtg gctaagttgt tgtttttatg ggtttttatt caattcataa gatgcagtag
     4741 atatgtcaaa agagagcttt taggtggatt ctttacagta gttagaccac gacaaaaatc
     4801 agcttttgaa tcgccacgga taatctagac acttccgaga cgttgataat actggttttc
     4861 atattctgtc ggtgacatct gatcgctgga accatgccga cgctggtaat gataccaaat
     4921 tgttgatttc gtgttttcac tgacaccagg attatcctga tgttacaagg attgaatgac
     4981 tacagattaa aatagtcatc aacaggttga cagtattacc aaaatttcta tgggattaat
     5041 tttttgcgag tgagtaatgt attctaatgc cttttggatg atggctgaaa aattagtcag
     5101 catcatcggt cttgtgtttg taaactccta tatggcaaaa tatataggac ctactgattt
     5161 tggtaagatt acttttgcta cgacaatatt tacgtttgta caggccatgg cttggtttgg
     5221 tgggcaaaac atccttttta aaaggatgag tgagaaaaga aaatcaggta ttgctcttgc
     5281 attacatact caaaagcagc gcagggttat ttttgcactc tcttcattga ttgccttgtt
     5341 ctatttgttt ttttttacag acaccctgac atttgttttt ggggtggcta actgcattgc
     5401 cacttattat atagtgatgg atattttcgc tgtatataat aatacgcagt taatatctag
     5461 aattaatgca ttaacaaatg gcgcagggtt gtgtattgct tatatattcc gctttttaat
     5521 ttcatacttg gagttgccag tatatacatt tagcattcct attatattaa ttccagcaat
     5581 accttatgtt attaggtgta tatattttta caataccgtt gaagacactt cggtattaaa
     5641 atcgaaaacg aagaaatata ataagtattt aatttcagcg ggagggtcgt tgttactatc
     5701 aaccctgtcc attgtggttt acacacaggt atcaaatatt tttttggcta aagtaacctc
     5761 atattctaat ttgggtattt ataatgtggc gctgactctt ggcagtgcgt attcatttat
     5821 ttcagttgcg ttaattacta gtttcttttc taaaatctat caagagaaag attttgaaaa
     5881 aatgtctaga cttctaacct caataaatag aattgtgatc gtgataacaa tttttatctt
     5941 atttggtttt tattttcttg ggccgtacat tatcagtatg ctctatggtt cggcctataa
     6001 aatggctgtt gagttggtgc caatcattat tctcgcgaca atgttttcat ccttgggagc
     6061 tatttgttat agatttataa taaaatactc tggctacaaa tacctttcat ataaaatgat
     6121 gttaactggc ttgctcagca tcccattctc atatgttatg attaaccgtt atgatgtttt
     6181 cggtgctgcg tattgttttc ttgtggtcga ggttctgtca tgcacattat taaattattt
     6241 ctttaggagt ggaatgattc taaggattca tttaaatgtt attgggttga tgaggcgttg
     6301 atggtggggg gggggcaggt ttatttatag gttgtgttgg taatggtgcc aacttactga
     6361 tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat cagttgtccc
     6421 tcctgttcag ctactgaagg cgtggtgcgt aacggtaaaa gtactgccgg acatcagcgc
     6481 tatctctgct ctcactgccg taaaacatgg cagctacagt tcacttacac cgcctctcag
     6541 cccggtacac accagaaaat tattgatatg gccatgaatg gcgtcggatg tcgcgccagt
     6601 gcacgcatta tgggcgttgg cctcaacacg attttacgac acttaaaaaa ctcaggccgc
     6661 agtcggtaaa ctcacgcata caaccgggca gtaacgtcat tgtttgcgcg gaaatggacg
     6721 aacagtgggg ttacgtcggc gctaaatcac gccagcgctg gttgttttac gcgtatgaca
     6781 ggatacggag gacagttgtg gtgcacgtat tcggtgaacg cacgatggcc acgctggagc
     6841 gtcttccggg cctgctgtcg gcctttgagg tcgtggtatg gatgagggat ggctggccgc
     6901 tttatgaacc acgcctgaag ggagaactgc acgttatcag caagcgatat acgcagcgta
     6961 ttgagcggca taacctgaat ctgaggcagc atctgtcaag gctgggcagg aagtcactgt
     7021 cgttctcaaa atcggtggag ctgcatgaca aagtcatcgg gcattatctg aacataaaac
     7081 acgatctgta agttggagtc attacctaca gtttcatatg tgggcgttta tgtaacatat
     7141 gtcgctgggc caactcttct ggtttctggg tttttatcgt ggattacaag gccgtaatct
     7201 gataatgccc ctaatcggtc agatctctag gatagtagca atacagcaag gaacttcttg
     7261 agatcctttc tgttgcgtcg taatctcatg tcctgcaaac aaaaaaacca ccgctaccaa
     7321 cggtggttcg tttgccggaa tcaagagcta ccaactcttt tttccgaagg taactggctt
     7381 tcacggagcg cagataccaa aacagtcctt ccagtgtagc cgcagtcagg ccaccacttc
     7441 aagaactctg tagaagcact ctactactct gctccgtgaa tcctgttacc agtggctgct
     7501 gccagtggcg ttttgtcgtg cctctccggg ttggactcaa gacgatagtt accggataag
     7561 gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc
     7621 tacccggaac tgagtgtcag gcgtggaatg agataaacgc ggccataaca gcggaatgac
     7681 accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccatcag ggggaaacgc
     7741 ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agattttgtg
     7801 atgcttgtca ggggggcgga gcctatggaa aaacgccccg gcggggcgtg gccttttcgc
     7861 ccttgtcctt agctttcgtt atgcttaccg ttaggttatc tgcttatctt ccctccgctc
     7921 gccgcagact catgaccgaa cggagtgagc gaatgagcga ggaagcggaa aacaatcaga
     7981 acgtacagta agcactttcg cgccggggcc ttcatgaaca aacaacaaaa gacagccgtt
     8041 aacatggcta aattcatcca ggatcagtcc ctgcttcttc tggacaggct taatgagctg
     8101 gatctggatc atgaggctga tttctgcgag aaactc
//
