LOCUS       NZ_AP023080            13096 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Acinetobacter baumannii strain OCU_Ac16a plasmid pOCU_Ac16a_3,
            complete sequence.
ACCESSION   NZ_AP023080
VERSION     NZ_AP023080.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00059694
            Assembly: GCF_001895125.2
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1
  AUTHORS   Oinuma,K., Suzuki,M., Sakiyama,A., Tsubouchi,T., Saeki,K., Sato,K.,
            Niki,M., Yamada,K., Shibayama,K., Kakeya,H. and Kaneko,Y.
  TITLE     Genomic analysis of Acinetobacter baumannii OCU_Ac16a, a strain
            carrying three acquired carbapenemase genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13096)
  AUTHORS   Oinuma,K., Kaneko,Y. and Suzuki,M.
  CONSRTM   Acinetobacter spp. genome sequencing consortium
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2020) Contact:Ken-Ichi Oinuma Osaka City
            University Graduate School of Medicine, Bacteriology; 1-4-3
            Asahimachi, Abeno-ku, Osaka, Osaka 545-8585, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: RS HGAP Assembly v. 3.0
            Genome Coverage                   :: 325.8x
            Sequencing Technology :: Illumina MiSeq; Pacific Biosciences PacBio
            RS II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 09:00:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,992
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,809
            CDSs (with protein)               :: 3,809
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 35 of 88
            Pseudo Genes (incomplete)         :: 50 of 88
            Pseudo Genes (internal stop)      :: 17 of 88
            Pseudo Genes (multiple problems)  :: 11 of 88
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023080.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13096
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="OCU_Ac16a"
                     /isolation_source="Tracheal aspirate from a hospitalized
                     patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pOCU_Ac16a_3"
                     /geo_loc_name="Japan:Osaka"
                     /collection_date="2015-12-19"
     gene            600..1529
                     /gene="repM"
                     /locus_tag="OCUAC_RS19765"
     CDS             600..1529
                     /gene="repM"
                     /locus_tag="OCUAC_RS19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005021644.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_057073160.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARGSGRGINANDP
                     LEVHAESYINQFNVARQTAYQALKDACKDLFARQFSYQEINKRGNVENVLSRWVSEIRY
                     IDDEATVKLIFAPAIVPLITRLEEQFTKYELQQISNLSSAYAVRLYELLIAWRSTGQTP
                     VIELTEFRNKIGVLDDEYTRMGNFKDRVLNLAIAQINEHTDITVKYEQHKKGRNISGFS
                     FSFKQKKVVTANANSKSSNTLDLFSKMTDAQRHLFANKLSELADMSKYSQGTESYAQFA
                     IRIAEMLQNQEKFEELLPYLKKVGFNAK"
                     /name="repM"
     gene            1590..2141
                     /locus_tag="OCUAC_RS19770"
     CDS             1590..2141
                     /locus_tag="OCUAC_RS19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005402002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_227552489.1"
                     /translation="MNTTKFSVMDASKAFKKSRTTIYEAIKNGELSRDNDGLIDLSELI
                     RVYGNPIGVQSSTRTEQVQRDAPVHVQSEVESVLKDQISLLKNQLDLANQREKSLMQHI
                     EDLTHRIEFKGVLEPSKQEDQNQSAMDTTINTNQTVRQNSSEVLATPEQTENKRIPVPE
                     RIEQEPEKRGFWSRFFLPNG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(2330..2959)
                     /locus_tag="OCUAC_RS19775"
     CDS             complement(2330..2959)
                     /locus_tag="OCUAC_RS19775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017399705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072689799.1"
                     /translation="MNKTTKAILVMMILIIGYYLFMVGVWKGWMDSWCQKKYCLDLLSF
                     DEYLSISIAIIGLVLVIKSLDGWKEQDKFINARNICNQLINFQTLCELDLLILIGQKQK
                     EMDLLASLEEQKKFLQKAFFELELFKVDRELGEKLEQSNFLCKNELKDAYKILNKCLHR
                     MYSDIDNQANNFKNIQSFLHIAIRDDIREVSKKLNKINEQLNHQAN"
                     /name="hypothetical protein"
     gene            3171..3539
                     /locus_tag="OCUAC_RS19780"
     CDS             3171..3539
                     /locus_tag="OCUAC_RS19780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_017399704.1"
                     /translation="MKKTILISTLIFTGSIHAADYKTALTGKTLYMKGGNCAGLSLSKN
                     AGLMGDQFPCTVDLPTRVRWINNDTFMMVEKDQTSSDKPPRVFLYKVKSLQGNKVVLSD
                     IWTGWNSFPDSDTTYTIK"
                     /name="hypothetical protein"
     gene            complement(3599..4111)
                     /locus_tag="OCUAC_RS19785"
     CDS             complement(3599..4111)
                     /locus_tag="OCUAC_RS19785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017399703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="WP_072689801.1"
                     /translation="MSNLENLTDTERLILANQYEILSKFEDSDYGVEYYKKLSNNLRLG
                     YKWLYQDHFQCLSDVLEDNDTELVVKTLSLYRTLKNNYEDLDDKSVIDEIKITFKGFDG
                     NNERRLMSFTQALKEDDRFTDIIESGQMNSHSPKKNQYQKMLSKWKELDEKEKLTLEEM
                     ISIIETY"
                     /name="YfbU family protein"
     gene            complement(4264..4875)
                     /locus_tag="OCUAC_RS19790"
     CDS             complement(4264..4875)
                     /locus_tag="OCUAC_RS19790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889724.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000571899.1"
                     /translation="MIEMNTRIYLRASTKDQDAKRALQILQDLNQNLNLGETIVYVENY
                     SGTKLDRPELNKLLSEANQGDTLLVESIDRLSRLTQRDFQELKRRIQEKGLRLVVADLP
                     TTYQMIQASDSITHSILELINNMLIDLLATMARLDNEKRIERIKQGLARSGYKPTGKKA
                     NEAKHKRIKELLVAGNMTKEEIAKAVNCGVATVYRVAKVI"
                     /name="recombinase family protein"
     gene            complement(5056..5583)
                     /locus_tag="OCUAC_RS19795"
     CDS             complement(5056..5583)
                     /locus_tag="OCUAC_RS19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="WP_000537572.1"
                     /translation="MGTVKLKSSDEVFQLKISLLGSDPEIWRTIQISSAATLSRLHKAI
                     QSAFDWEDSHLHEFTTIKHEKINKRQKLKEVLRVGKKIIYTYDFGDCWEHLITVESRQS
                     PDLNKKYPCCIDGQNHAPFEDIGGIFGYLDILDALQDPKHPRYDDYMQIIEDEIGEIEF
                     DPTYFNSHDIKF"
                     /name="plasmid pRiA4b ORF-3 family protein"
     gene            complement(5699..5989)
                     /locus_tag="OCUAC_RS19800"
     CDS             complement(5699..5989)
                     /locus_tag="OCUAC_RS19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647750.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="WP_005093398.1"
                     /translation="MVKVADLPSFDMAESLKTEEDIVMYLNMVLEENDPAELAHALGVI
                     AKARGMTQIAKEAGIGREALYKALRHDSAPRFDTINRVVNALGLKLTVQHA"
                     /name="addiction module antidote protein"
     gene            complement(5991..6287)
                     /locus_tag="OCUAC_RS19805"
     CDS             complement(5991..6287)
                     /locus_tag="OCUAC_RS19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004647749.1"
                     /translation="MIEIKRLPEFDEWLDGIKDNMTRIRLNRRLDKVQRGNWGDIKPLQ
                     DGVWEMREFFGSGWRMYYIQHGDVVIVMLGGGDKSTQQQDIDRAVKLSKTLED"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6441..6578
                     /locus_tag="OCUAC_RS20030"
     CDS             6441..6578
                     /locus_tag="OCUAC_RS20030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227552490.1"
                     /translation="MTLKGLDIQEDCIKAISNESLIFDIKNKEFLEDIENLLLQYFSKF
                     "
                     /name="hypothetical protein"
     gene            7240..7527
                     /locus_tag="OCUAC_RS19815"
     CDS             7240..7527
                     /locus_tag="OCUAC_RS19815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438825.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            7514..7828
                     /locus_tag="OCUAC_RS19820"
     CDS             7514..7828
                     /locus_tag="OCUAC_RS19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_004728120.1"
                     /translation="MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(7878..8114)
                     /locus_tag="OCUAC_RS20035"
     CDS             complement(7878..8114)
                     /locus_tag="OCUAC_RS20035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227552486.1"
                     /translation="MAIAVITVYTAQLANYYQSLILFSFIFMLISLPCLFIWAYLGKIT
                     KKILSTAHHMVLFNRVLGTVLLISVWYPILHAI"
                     /name="hypothetical protein"
     gene            complement(8125..8484)
                     /locus_tag="OCUAC_RS20040"
     CDS             complement(8125..8484)
                     /locus_tag="OCUAC_RS20040"
                     /inference="COORDINATES: protein motif:HMM:NF013933.3"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="WP_227552487.1"
                     /translation="METILAFCAFSFVASITPGPTNFLILSTSHQFSIKKTLGLIFGGS
                     IGAASLVLITGLGIGTTLNEYPKIQLILSFTGGIWLSYIGWKIFNYRPDLESKIQNQNK
                     KNGFFYRLLFTVYQP"
                     /name="LysE family transporter"
     gene            complement(8534..9362)
                     /locus_tag="OCUAC_RS19830"
                     /pseudo=""
     CDS             complement(8534..9362)
                     /locus_tag="OCUAC_RS19830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073659.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]; GO:0043565 - sequence-specific DNA
                     binding [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /name="AraC family transcriptional regulator"
     gene            complement(9536..10242)
                     /locus_tag="OCUAC_RS19835"
                     /pseudo=""
     CDS             complement(9536..10242)
                     /locus_tag="OCUAC_RS19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586042.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            complement(10333..11175)
                     /locus_tag="OCUAC_RS19840"
     CDS             complement(10333..11175)
                     /locus_tag="OCUAC_RS19840"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002002480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class D beta-lactamase
                     OXA-58"
                     /protein_id="WP_002002480.1"
                     /translation="MKLLKILSLVCLSISIGACAEHSMSRAKTSTIPQVNNSIIDQNVQ
                     ALFNEISADAVFVTYDGQNIKKYGTHLDRAKTAYIPASTFKIANALIGLENHKATSTEI
                     FKWDGKPRFFKAWDKDFTLGEAMQASTVPVYQELARRIGPSLMQSELQRIGYGNMQIGT
                     EVDQFWLKGPLTITPIQEVKFVYDLAQGQLPFKPEVQQQVKEMLYVERRGENRLYAKSG
                     WGMAVDPQVGWYVGFVEKADGQVVAFALNMQMKAGDDIALRKQLSLDVLDKLGVFHYL"
                     /name="carbapenem-hydrolyzing class D beta-lactamase
                     OXA-58"
     gene            complement(11368..12072)
                     /locus_tag="OCUAC_RS19845"
     CDS             complement(11368..12072)
                     /locus_tag="OCUAC_RS19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067790.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1008 family transposase"
                     /protein_id="WP_001067790.1"
                     /translation="MNPFHGRHFQGEIILWAVRWYCKYGISYRELQEMLAERGVNVDHT
                     TIYRWVQRYAPEMEKRLRWYWRNPTDLHSWHMDETYIKVKGRWTYLYRAVDQRGHTIDF
                     YLSARRNSKSAYCFLGKIFNTVKKWQIPRVINTDKAATYGHALSRLKREGKCPVDIEHR
                     QIKYKNNVIECDHGKLKRIIRATLGFKSMKTAYATIKGIEVMRALRKGQASSFYYGQPQ
                     GEVCLINRVFGL"
                     /name="IS6-like element IS1008 family transposase"
     gene            12131..12619
                     /locus_tag="OCUAC_RS19850"
     CDS             12131..12619
                     /locus_tag="OCUAC_RS19850"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227552488.1"
                     /translation="MPPKLGVGKQNETEEYLADIQKIRLHNAKEREMEQKQRQQTQKHE
                     HFYSCLNEPYTSLEVMYYLNYLAKKAKNQKELKQDFNEIIKTQKPMLQNEHWNLNRMDY
                     QLKNSLKYPDPQIEFNPVTALKPEAQLSKVIQTNQHLEPTRPSIQPKPKNDDYDFGGP"
                     /name="hypothetical protein"
     gene            complement(12639..12947)
                     /locus_tag="OCUAC_RS19855"
     CDS             complement(12639..12947)
                     /locus_tag="OCUAC_RS19855"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_057073233.1"
                     /translation="MNFFKENEEHILLYSKISYFDKTAYLHLLFLNGELTLKSTDLISV
                     SYEQIYLLKENKNMAIRIDPRSEEKEINKLQRLFKEALSYESTCLSCVRRQHSLYTI"
                     /name="hypothetical protein"
     ARG             complement(10337..11175)
                     /name="blaOXA-58"
                     /gene="blaOXA-58"
                     /gene_name="carbapenem-hydrolyzing class D beta-lactamase
                     OXA-58"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          601..1290
                     /name="rep_cluster_2314"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.451"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttcatgtcct atggatatgg gatatgcaaa aagcgttatt acttaagtag cgacactttt
       61 tggaatatca cctaacttca tagagcgagt gtctacgagc gaactgacat cattaaaatt
      121 tcgcctgctt ttttccctga ttacaggctc ccctttactt acgtgattta gaatcagagc
      181 gtcacgaagt gcgcgaacga tagagctttt gctttttatt caagctacaa cgaaattaag
      241 taaaaaattg ccccctagaa tcgagcgaaa gcgagattca atagagtttg agcgtagcga
      301 aaacataggg caatttttca tgacctgggc ttttaattat ttttaaagtt tttaaatgct
      361 tttagacata ctgaaagcct ttattcacaa tggttacaga cgctatatga ctacgtttac
      421 ctaccaatat gactacgttt acctaccaat atgactacgt ttacctacca atatgactac
      481 gtttacctac cataaatcta caaaatattt ttagtagatt taactacgtt taccttgcat
      541 aaatctacat aattattaat atagacttat attcataaat aataatgtag ataaataaga
      601 tgcgggattt agttgttaaa gataatgcat taatcaatgc aagctataac ttagatttag
      661 tagaacagcg tttaatccta ttggctattg tcgaagcaag aggtagtggt agagggatca
      721 atgcaaatga tcctttagaa gtccatgcag agagctatat caaccagttt aatgttgcaa
      781 gacaaacagc ctaccaggcc ttgaaagatg cttgtaaaga cttattcgcc cgacaattta
      841 gctatcaaga gatcaacaaa agagggaatg ttgagaatgt tctaagtcgt tgggttagcg
      901 aaattagata tatcgatgat gaagcaacag taaagctgat ctttgcacca gctattgttc
      961 cccttattac acgtttggaa gaacagttca caaagtatga gttacagcaa attagtaatc
     1021 tcagtagtgc ttatgctgta cgtttatacg agttattgat tgcttggcgc agtacaggtc
     1081 aaacaccagt tattgaacta acagaattta gaaacaagat aggtgttctc gatgatgaat
     1141 acacaagaat gggcaatttt aaagatcggg tactaaatct agctattgct caaattaatg
     1201 aacacactga cattactgtt aaatacgaac agcataaaaa gggacgtaac atttcaggtt
     1261 tttcattttc tttcaaacaa aagaaagtag ttacagctaa tgcaaatagt aagagttcaa
     1321 atacccttga tcttttctcg aaaatgaccg atgcacaacg ccatttgttt gctaataaac
     1381 tgtcagaact tgctgacatg agtaagtact ctcaaggtac cgaaagctat gcacaatttg
     1441 ctattcgaat tgctgaaatg ctacagaatc aagagaaatt tgaagaacta ctaccgtatc
     1501 ttaaaaaagt aggatttaac gcaaaataaa cttcatgttc aggtaggatg ttcagatgaa
     1561 catccgtgta catgcactga gggactacat tgaacacaac aaaatttagc gttatggacg
     1621 ctagtaaagc ctttaaaaaa tcacgaacaa caatctatga agctataaaa aatggtgaat
     1681 tatcaaggga taatgatgga cttatagatc tatcagaatt aataagagtt tatggaaatc
     1741 caattggtgt tcagtccagt acacgtactg aacaagttca aagggatgca cctgtacacg
     1801 tccagtctga ggttgaaagt gtcctaaaag atcagatttc cttacttaaa aatcagttgg
     1861 atttagcaaa tcaaagagaa aagtctttga tgcaacatat cgaagatctt actcatagaa
     1921 ttgagtttaa aggtgtccta gagccatcta aacaagaaga tcaaaaccag tctgcaatgg
     1981 atactacaat caatacaaat cagactgttc gacagaatag ctctgaggta ttagctactc
     2041 cagagcagac agaaaacaag cggattccag taccggagcg cattgaacag gaaccggaaa
     2101 aaagaggatt ctggagccga ttttttctgc cgaatggctg aggggattgt tcacctgatc
     2161 ttattaaaat cgtggaacat tgctcaaaag ttaagcgtgg aacatgaaga atcaacaaaa
     2221 aagcccaaat cagctgagtt gggcttttca catcgacata agattatgaa ttataagaaa
     2281 taaaaaattg catttcgtat aaggtgtatt atgttaaatt tagatattct taattagctt
     2341 ggtgatttaa ttgctcattt attttattta atttcttact gacttctcta atatcatcac
     2401 gaatagctat gtgtaaaaag ctttggatat tcttaaaatt atttgcttga ttgtctatgt
     2461 ctgagtacat tcgatgcaag cacttattta atattttata agcatctttt aactcgtttt
     2521 tacataagaa attagattgc tcaagctttt cccctaactc tctatctact ttaaataatt
     2581 caagttcaaa aaaagctttt tgtagaaact tcttctgttc ttctaaagaa gctaataagt
     2641 ccatttcttt ttgcttttga ccaattaaaa ttaagagatc taactcgcat agcgtttgaa
     2701 aatttataag ctgattacat atatttctcg catttataaa tttatcttgt tccttccaac
     2761 catctaatga tttgattaca aggactaaac caatgattgc tatcgaaatt gataaatact
     2821 catcgaacga caataaatct aagcaatatt ttttttgaca ccagctatcc atccatcctt
     2881 tccacacgcc taccatgaat aaatagtaac caatgattag tatcatcata actaagatag
     2941 ctttagtggt tttattcata gttaaatact aattatccta atgtgggaac tgtgataagc
     3001 ataaaagtag ttttttctac tatagcggta tactgctttt gtaactcaac ttttttagaa
     3061 ctattatcca taagaaaatt attaaaattt ttcaaaaata taattaatta tattagcatg
     3121 agctagcatt attacactgc aattttaata tattgaaatg agggagaaaa atgaaaaaaa
     3181 ctattctaat tagtacctta atatttacag gttctattca tgctgcggac tataaaactg
     3241 ctctgactgg aaaaactctt tacatgaaag gcggaaattg tgcaggccta tctctttcaa
     3301 aaaacgctgg tctaatggga gatcaatttc cctgtacggt agacttacca actagagtac
     3361 gatggataaa taatgatact ttcatgatgg ttgaaaaaga tcaaacttca tcagacaagc
     3421 ctccacgagt attcctatat aaagtaaagt ctctacaagg aaacaaggtt gttctcagtg
     3481 atatatggac aggatggaat agctttcctg atagcgatac aacttacaca atcaagtaga
     3541 aatagaactt taatggtgtg atacaagtgg gattattaat cccacttttc tgaatttttt
     3601 aataggtttc tataattgaa atcatttcct caagtgtaag tttttctttt tcatctaatt
     3661 ctttccactt actcagcatc ttttgatatt ggtttttctt tggactatga gaattcattt
     3721 gaccactttc aataatatct gtaaatctat catcttcttt caatgcttgc gtaaagctca
     3781 ttaaacgtct ttcgttattg ccatcaaatc ctttaaaagt aatcttgatt tcatcaataa
     3841 cacttttatc atccagatct tcataattat tttttaaagt tctataaagg cttaatgtct
     3901 ttacaactaa ttcggtatca ttgtcctcaa gaacatcact tagacattgg aaatgatctt
     3961 ggtacaacca tttatagcca agtcttaagt tatttgataa ttttttataa tattcaacgc
     4021 cataatcact atcttcgaac ttactcaata tttcatattg gttcgccaga attaatcgtt
     4081 ctgtatcagt taaattttct aaattagaca tagatcatgt tcatataaat tatttaagat
     4141 tctgatattg gtgcctattt ttaaaattca ataccctatt taacataaaa tctcttatgt
     4201 gaagcaatct ttgtaactcc aaaatagaaa tgtccggttt ctccaaagta aaaatgtccg
     4261 cttttagata actttagcaa ctcgatagac agttgcaact ccacaattca ctgcttttgc
     4321 aatttcttcc ttagtcatat tgccagccac cagcagttct tttattcgtt tatgtttagc
     4381 ctcattagcc ttcttacctg ttggcttgta acctgaacgc gctaggccct gcttaatacg
     4441 ttctatacgt ttctcattgt ccaggcgagc cattgttgcc aggagatcaa tcaacatatt
     4501 attgatgagt tccagaattg aatgagtaat gctgtcactg gcttgaatca tttggtaggt
     4561 agtaggaaga tctgctacga ctaaacgaag tcccttttcc tggatcctgc gcttcagctc
     4621 ttgaaaatct cgttgggtta ggcgtgataa gcggtcaata ctttcaacta acaaggtgtc
     4681 accctgattt gcttctgaca gtagcttatt caattcaggt cggtctaact tcgtaccact
     4741 atagttttcc acgtacacaa tggtttcgcc caaattcaag ttttggttta gatcctgaag
     4801 aatctgcaaa gccctttttg catcttgatc tttagttgaa gctcgtagat aaatacgtgt
     4861 attcatttct atcatttagt cttaattcga ttaggttaat gataatacta tcatttaatt
     4921 attttactaa gtttaatgac aggtattata tgcgatttgg cttgctatca ttcagctata
     4981 ctcttttgat aaaaaaagca tgactaaaag ccatgccttg ggaattcgta taacagccct
     5041 tatgttaaat caggtttaaa acttaatatc atgtgaatta aagtaggtcg gatcaaactc
     5101 tatctctcct atttcatcct cgataatttg catgtaatcg tcatatcttg gatgtttggg
     5161 gtcttgtaat gcatctaaaa tatcaagata tccaaaaatt cctccaatat cttcaaatgg
     5221 agcatgattt tgaccatcta tacaacatgg atatttttta tttaaatcag gagattgtct
     5281 gctttctaca gtaatcaaat gttcccaaca atcgccaaaa tcatatgtat atataatttt
     5341 tttacctaca cgtagaactt ctttcaattt ttgtctttta ttgatctttt catgcttaat
     5401 agtcgtgaat tcatgtaaat ggctatcttc ccaatcaaaa gcagattgaa ttgccttatg
     5461 gagcctagat aaagtagctg ccgatgaaat ttgaatagtt ctccagattt caggatcact
     5521 acctagcaga ctaattttta attgaaaaac ttcatcagaa gatttgagct ttacagttcc
     5581 cataacagcc tagttctcaa aaaatagttt tagtaaatct aaaattaaca taatacacct
     5641 tatacgaaac taaaaatggg cgcctttagc tcccattttt attatatttt ttaaagtctt
     5701 aggcgtgttg tactgtgagt tttaagccta aagcattcac tacacgatta atcgtatcaa
     5761 aacgtggtgc tgaatcatga cgtagggctt tgtagagtgc ctcacgccca attcctgctt
     5821 ctttagcgat ttgagtcatg ccacgagctt tggcaataac accaagtgca tgagctaatt
     5881 cagctggatc attctcttca agaaccatat tgagatacat cacaatgtct tcttcagttt
     5941 tgagtgactc agccatatca aaacttggta aatcagctac tttaaccatt ttaatcctcc
     6001 aacgttttcg atagtttgac tgcacgatcg atgtcttgtt gttgagttga tttatctcca
     6061 ccaccaagca ttacaatcac aacgtcacca tgttggatgt aatacatcct ccatccagaa
     6121 ccgaagaatt ctctcatttc ccaaacgcca tcttggagag gcttaatgtc tccccagttt
     6181 ccacgttgaa ccttatctaa tcgacgattc aggcggatac gggtcatatt gtctttaatg
     6241 ccatccaacc attcatcaaa ttctggaaga cgtttaattt caatcatgag gattccttat
     6301 cttctcacta tattgtattc gatcgaatac aatatgcaag taatctatac gaacatccat
     6361 taatttcgta taaccgccat tatgttaagt taatcaaaat tcttaatgca ccaaaaccta
     6421 aaaatttaaa tggagtaaaa atgactttaa aggggttaga tattcaagaa gattgtatca
     6481 aagctatttc taatgaatct ttaatatttg atattaagaa taaagagttt ttagaggata
     6541 tagaaaatct tttattacag tatttttcaa aattttagta atgatttaaa tggaattgag
     6601 tttgataatt tttctgtgga gttttggttc gatgcaggac aattgattat ttatcctgaa
     6661 aaagatttat tagatcgtaa accctttgaa tctgaatatg atttagatag attagacctt
     6721 atttttatct agtttgtgaa gaatatagga tatattttga tgacttaata tctcgaaaag
     6781 taagcgatca agtatctgaa aaagaagcta tttctaaaac taatgacgta attgattgtg
     6841 taagcaaagc tataaaaaat ataaatgatg aaaacaatct tttaaagatg ttaggcaggc
     6901 ctaaattaga aataagatat ttcggtgtaa cgaaagaaga acttttagca aaagaaattc
     6961 tcgtgaaatg aagtagatat ttttgtcgta ttaaggaaaa ctaaaattaa cataatgggc
     7021 gttatacgaa atgcacaaat tgaaaaatat aaaaatcaaa aacttatgat ataaattatt
     7081 tgctcgatga tgttcaaaag ccgacttgga aacaagtcgg cttttttatg agcagttttg
     7141 atagttcttg ccaataaatt tgactaaaaa ttacgcttct caacttgagt tatacaattt
     7201 tgtgatacat tattgtatat acaaatttga atgtttttta tggaacagta ttttgaatgg
     7261 gatgaggcta agaatcgaaa gaatcagaaa aagcatgata tctctttcga aactgcaagc
     7321 cttgtttttg aagatccatt acggatctca atccaagaca gacataccga tggtgaagaa
     7381 cgttggcaaa ccattggacg agttaaaggc gtactaatgt tattggtggc tcacaccatt
     7441 tttgatgaag atgactgtga aatcatacga atcattagtg caaggcaagt gactaaagcg
     7501 gagcgaaaca aatatgagca tggttagata ctcacgcaaa gagctgaatg agaaatttgg
     7561 tgagaagcaa gatgctgaaa ttcaacgctt gcttgctaaa ggcacggttc ctgatgatca
     7621 gctagatcta tcagatattc ctgaaattac cgattggagc agtgctgtac gccaaaatca
     7681 attttaccgc ccagtgaagc aacaaacttc aattcggtta gatgctgatg tacttgcttg
     7741 gttcaaagca caaggcaaag gttatcaaac acgaatgaat gaaattttac gggatgctat
     7801 gctgaaagaa ttaaaaaatc atcaataaaa atgggagctt aagctcccat ttttgacttc
     7861 gcataacgcc cattatgtta aatagcatgc aatattggat accatactga aataagtaaa
     7921 acagtaccta atactcgatt aaagagcacc atatgatgag ctgtagaaag tatttttttt
     7981 gtgatttttc ctaaataagc ccaaataaat aaacaaggta gagaaatcag cataaagatg
     8041 aatgaaaata gaatgagtga ctgatagtag ttagctagtt gtgctgtata aacagtaata
     8101 acagcaatag ccatcaacca gcttttaggg ttgataaact gtaaaaagaa gcctgtaaaa
     8161 aaaaccattt ttcttatttt gattttggat tttagattct agatcaggtc tataattaaa
     8221 aattttccat ccaatatagc ttaaccaaat gccgccagtg aaacttaata ttaattgaat
     8281 cttggggtat tcattgagtg ttgtaccaat tccaagtcct gtaattaaga ctaatgatgc
     8341 agcaccaata cttccaccga agattaagcc taatgttttt ttaatactaa attgatgact
     8401 tgtactaaga ataaggaaat tggttggtcc aggagtaatt gatgcaacaa aagaaaaggc
     8461 acaaaaagct aaaatcgttt ccaaaataaa atccaaaaat taataaacta tttggctagt
     8521 ttgataaata tcttcaagat atatctagaa catttgtgca cgctttttga tattgattca
     8581 atgttatgcc ataacaacgt ttaaaccatc gacctagatg actttgatca gaaaaacaaa
     8641 gatatgaagc aacagttgtt gctgataatc cttttcttaa caattctttt gccttattta
     8701 aacggagttg gatcagatat tgatgtggag aacagttaaa gtttttttta aatacacggt
     8761 taatataaaa acgatctgtt ttcaccaatg ttgagagtgt ttgtaagtct aaatcatgaa
     8821 agagactttc atgtaaaatt tcttttagct ttaatgcaat attaggtaat gtctggatag
     8881 acttttcacg tttatataaa atttttttat ttacctaagt tttctaataa aagatctaaa
     8941 tacgtatctt tcatcaattg agattcattg ttattcaata tattgtaggt tgaaagtatc
     9001 aaatgtgaaa gttgaggatc agaacgtagg gttgattcga tagcaagttc gataggctca
     9061 ttaaatattc cttcatagct tttttttaac catgctggat ctaaatgcat cattttataa
     9121 gtgaagccga gaggatctgg tgcattacca tcatgaactt cttcaggttc aagcatgaaa
     9181 gtttgacctt gatagctatc aataattttt tttctacaat tgaattgttg atgtcctaac
     9241 tctgttaccc ctataagata acttgagtgt agatgaggat cgtatgcaaa gcctttgaag
     9301 tgggcatgaa ttagttctat acctgtagac ttatcctgtg tgacattgac ccaattgctc
     9361 atatttactt aattagtcct aatggattta gcttatcaaa aattatctaa tgaagaaatt
     9421 ctaaaattaa cataatacac cttatacaaa atgcccttgt aagcccttaa tcaagggcat
     9481 ttttagtctc tacttaacat aaaattttgg tggtgcttca aaaagtatgc tgcctttaaa
     9541 tgaagccatt attgatgtaa aagaaaagaa ggctaaatca aaagctttaa aatggttttc
     9601 aagctttttt gaaaaattac agcttcttct gaagctacgc ctaattctat gccttatttt
     9661 gcaattattg ccttcaatac ctacagtaaa aaatttacca atactttgct taccattttt
     9721 aaaagcagtg atgaaactgt cccaatgatc acttgcaatt cgggtgtagt gaatatctaa
     9781 ttgtttaagc tttgtcttca attgttagac tgtagctaaa tctcgtttac cccaaacata
     9841 agcaacaatt tcacctgttt ctcgatgata ggcgtaaata agccattgtt tattcttttt
     9901 atttccaaca aaattccaga actcatctac ttcgagagat tcataatgac tttgctgagg
     9961 ctgaatttca taggttgatt cagttaaagt acgtaaaact ttaccgatac tgatgcgctc
    10021 aacttcagca atatctcgta taccgctgcc tctgaccatc aactgtaata ttttacgagt
    10081 aatacctgac ttacatccta gatagctcag tgcatgatca ccaataaact gacgtttaca
    10141 gtctttgcac tgatagtttt gtttcccatc tactttgata ccatttttct ttatactatc
    10201 actgaggcag gttggacatt tgattgctag agttatttgc atttctctat tttatcaaaa
    10261 tccaatcggc tttttcttca gcatactttt tgaaacacta ccaaaaatag attaacctca
    10321 aacttctaat tcttataaat aatgaaaaac acccaactta tctagcacat ctaaagacaa
    10381 ttgtttacgt agagcaatat catcaccagc tttcatttgc atatttaaag caaatgccac
    10441 cacttgccca tctgcctttt caacaaaacc cacataccaa cccacttgcg ggtctacagc
    10501 cattccccag ccacttttag catatagacg attctcccct ctgcgctcta catacaacat
    10561 ctctttcact tgttgctgaa cttcaggttt aaaaggcaat tgcccttggg ctaaatcata
    10621 cacaaacttt acttcttgta taggtgtaat tgtcaaaggc cctttcaacc aaaattgatc
    10681 aacttccgtg cctatttgca tattgccata accaatacgt tgcaattcac tttgcattaa
    10741 gcttggacca atacgacgtg ccaattcttg atatacaggc actgtagatg cttgcatggc
    10801 ttcgcccaaa gtaaaatctt tgtcccatgc tttaaaaaaa cgtggctttc catcccactt
    10861 aaatatttct gtagatgttg ctttatgatt ttctaaacca attagtgcat tggcaatttt
    10921 aaatgtagat gcaggaatat aagctgtttt tgctcggtct aaatgcgtgc catatttttt
    10981 aatattttga ccatcatatg tgacaaacac agcatcagct gagatttcat taaaaagcgc
    11041 ttgaacattc tgatcgatga ttgagttatt cacttgtgga attgtacttg tttttgctcg
    11101 actcatacta tgctcagcac aagccccaat acttatgctt aagcaaacta aactcaatat
    11161 ttttaataat ttcatgatat acaactttaa aatttggtag tgtttcaaaa agtatgctga
    11221 agaaaaagcc gattggattt tgataaaata gagaaatgca aataactcta gcaatcaaat
    11281 gtccaacctg cctcagtgat agtataaaga aaaatggcac tgttgcaaat agagatttga
    11341 gtcgatgaag ttccttttta aaagtgctta gagaccgaaa accctgttga ttagacacac
    11401 ttcaccttga ggctgaccat aataaaatga cgatgcttgt cctttacgta gtgcacgcat
    11461 gacttcaata cctttaattg tggcataagc cgtcttcata gatttgaatc ctaatgtggc
    11521 cctgatgatc cgctttaact taccatgatc acattcaatg acattatttt tatacttaat
    11581 ctgcctgtgc tcaatatcta ctggacattt tccttctcgc tttaaccgtg ataaagcatg
    11641 gccataggtc gctgctttat ctgtattgat gacccgtgga atttgccatt ttttcaccgt
    11701 attgaagatc tttcctaaaa aacaataggc tgatttactg ttccgtctag cggaaaggta
    11761 aaagtcaatc gtatgaccac gttgatcaac tgcacgatac aggtaagtcc atcgcccctt
    11821 cactttgata taagtctcat ccatatgcca tgaatgtaaa tctgtaggat tacgccaata
    11881 ccagcgtaag cgtttttcca tttctggagc ataacgctga acccaacgat aaattgtggt
    11941 gtggtcaaca ttcacacctc gttcagcgag catttcttga agttcacgat agctgatgcc
    12001 atatttacaa taccaacgaa cagcccaaag aatgatttca ccttgaaagt gccgaccatg
    12061 gaagggattc atctgctgta tctcgaaata aataagatat ttagcttatc atgtcagcct
    12121 atttgcaaca gtgccaccca aattaggtgt gggaaaacag aatgaaacag aggaatacct
    12181 ggctgatatc cagaaaatcc ggctgcacaa tgccaaagaa cgagaaatgg aacaaaagca
    12241 gcggcaacag acgcagaagc atgaacattt ctattcatgc ctgaatgagc cgtatacctc
    12301 acttgaagtc atgtattact tgaattattt agccaagaaa gccaaaaacc aaaaagaact
    12361 caagcaagac ttcaacgaaa tcatcaaaac gcagaagcca atgcttcaaa acgagcactg
    12421 gaacctgaac aggatggatt accagctgaa aaattcactc aaatatccag atcctcaaat
    12481 cgagttcaac cccgttacag ccttaaaacc agaggctcaa ttatcaaaag tgattcaaac
    12541 gaaccaacat cttgaaccta ctcgcccctc catacagcca aaacctaaaa atgatgacta
    12601 tgattttggt ggtccttaaa tttgatgttc ttaataccct agatggtata gagagagtgt
    12661 tgtctacgaa cgcaggataa gcaagtcgac tcataactca aagcttcttt aaaaagacgc
    12721 tgcagtttat taatttcttt ttcttcagag cgaggatcta ttcgtatagc catgttttta
    12781 ttctctttga gcaaatagat ctgttcataa ctaacactta tcagatcagt actttttagc
    12841 gtcagttcac catttaaaaa tagtaaatgt aaatatgccg tcttatcgaa ataactaatt
    12901 tttgaataca ataaaatatg ttcttcattt tctttaaaaa agttcatgtg aactctctct
    12961 tttgggcaga aaatggagca taacaatttt aaatttttca tgatttcata cgttgcattt
    13021 ttgaaaaaaa agttactttt ggttgaataa tgttcaattt ataaaaatta aaactaccat
    13081 aatatgtgta aaacat
//
