LOCUS       NZ_AP023004             8230 bp    DNA     circular CON 14-MAR-2024
DEFINITION  Bacillus cereus strain 30077 plasmid p30077_4, complete sequence.
ACCESSION   NZ_AP023004
VERSION     NZ_AP023004.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203343
            Sequence Read Archive: DRR206383, DRR206407
            Assembly: GCF_021655355.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8230)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 425x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655355.1-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 04:00:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,939
            CDSs (total)                      :: 5,787
            Genes (coding)                    :: 5,503
            CDSs (with protein)               :: 5,503
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 153 of 284
            Pseudo Genes (incomplete)         :: 140 of 284
            Pseudo Genes (internal stop)      :: 45 of 284
            Pseudo Genes (multiple problems)  :: 49 of 284
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023004.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="30077"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="p30077_4"
                     /geo_loc_name="Japan:Tottori"
                     /collection_date="2016"
     gene            complement(303..1742)
                     /locus_tag="L6491_RS29520"
                     /old_locus_tag="BC30077_p41"
     CDS             complement(303..1742)
                     /locus_tag="L6491_RS29520"
                     /old_locus_tag="BC30077_p41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053955.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="WP_236791731.1"
                     /translation="MNLSIQDELQLFSEELYRHLTPSLLEELAKELGFVKRKRKFSGNE
                     LATICIWVSQRTASDSLVRLCSQLHAATGTLMSPEGLNKRFDKKAVEFLKYIFSALWKS
                     KICETSAISSAALTYFQRIRILDATIFQVPKHLAHVYPGSGGCAQTAGIKIQLEYDLHS
                     GQFLNFQIGPGKNNDKTFGTECLDTLRPSDLCIRDLGYFSLEDLDQMDQRGAYYISRLK
                     LNHTVYVKNPFPEYFRNGTVKKQSQYIQVDLEHIMNNLKPGQTYEIKEAYIGKNQKLFT
                     RVIIYRLTDKQIQERRRKKQSYTESKKGITFSEKSKRLTGINIYVSNTPEGVVPMEQIH
                     DFYSLRWQIEIIFKTWKSLFQIHHWQTIKKERLECHVYGRLIAIFICSSTMFKMRKLLL
                     QKNKRELSEYKAIGMIQDHVSLLYQAIQRNTQNLTKILIRLFDLLQKNGRKSHRYEKKT
                     IFDIMGVVYEYNGFGKQKKAA"
                     /name="IS4 family transposase"
     gene            complement(2072..2449)
                     /gene="qacH"
                     /locus_tag="L6491_RS29525"
                     /old_locus_tag="BC30077_p42"
     ARG             complement(2076..2449)
                     /name="qacH"
                     /gene="qacH"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacH"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(2449..3000)
                     /locus_tag="L6491_RS29530"
                     /old_locus_tag="BC30077_p43"
     CDS             complement(2449..3000)
                     /locus_tag="L6491_RS29530"
                     /old_locus_tag="BC30077_p43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_000028030.1"
                     /translation="MSISKHANKKKNILIAASEIVKEEGVVKLTLEAVAQRAGVSKGGL
                     LYHFPSKEALIKGMVEDWTNNYFECINTLVNNDDDNAIGKWNRAYLKSTFSDLENNNLN
                     SALMATMFINPDLLDEFRQRYDILHTKLITDGIDPVKITITRLSIDGLWFSEIFGMAPL
                     NEELKTQVFDELINMIQEDE"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            4195..5307
                     /locus_tag="L6491_RS29535"
                     /old_locus_tag="BC30077_p44"
     CDS             4195..5307
                     /locus_tag="L6491_RS29535"
                     /old_locus_tag="BC30077_p44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002019875.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_000036645.1"
                     /translation="MSKITNDIDLKIDEVYEKVKDMKITFDENNTTKKQLTDSTINTYC
                     GIVKSYNNWLIQTHGISVDRAKPRHAMEYLETKIQAFEKGNASAFSMRQMAHAFHSFRV
                     ASAETGTFKHTCKVADKRDMLNTLTERGIFRKSADSTVLKANHSDYEKVQKELNVATTS
                     KHLREDIKTVHELQRYAGMRVHEAVKLKVSDIDFKSGTVYIKGKGGHERTVKIESKELL
                     EKLKNLCTGKSERSQVIIARKRDKNQLTNEEFGKRAKEEIRGAAVNAGVDRNGKRYTTH
                     SGRKAYAQAQMDKYKSYSKNQLKKEVAKRCQDKKTKQKYDAVIKNIRKKITEGSKNEGR
                     KLTNKEMCQFLVSLDLGHYRLDVVRYYADY"
                     /name="tyrosine-type recombinase/integrase"
     gene            6120..6527
                     /locus_tag="L6491_RS29540"
                     /old_locus_tag="BC30077_p45"
     CDS             6120..6527
                     /locus_tag="L6491_RS29540"
                     /old_locus_tag="BC30077_p45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000037919.1"
                     /translation="MSKKISIEEMTQEMKEKFTISLEDAHKILLDHGVTKSTNTQVTMR
                     WIRDGKIDALLIGKGRVETRKWLINKESLMEFVAFERLTLGDFKAMKEELEMLRAFKFE
                     VEKKPKRATGTRQKKDAATNTSKEKKEVPTK"
                     /name="hypothetical protein"
     gene            6541..6978
                     /locus_tag="L6491_RS29545"
                     /old_locus_tag="BC30077_p46"
     CDS             6541..6978
                     /locus_tag="L6491_RS29545"
                     /old_locus_tag="BC30077_p46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000094076.1"
                     /translation="MSSFRWYEENEVIGKTIIEEDDLTLLEAGKIYIDAIFCERIRFPV
                     QKNEQPIGFNFVYEYNVKKYFGLYDRSFMKIIKQEDLEEYHMRMIDFHVHRLSLGEELD
                     DCPIIGICYGSGPLITPRLEQVYDVSKTKAYRKYRLEKLFD"
                     /name="hypothetical protein"
     gene            7229..7849
                     /locus_tag="L6491_RS29550"
                     /old_locus_tag="BC30077_p47"
     CDS             7229..7849
                     /locus_tag="L6491_RS29550"
                     /old_locus_tag="BC30077_p47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000963189.1"
                     /translation="MAIDFSQADRNARKRDLEKEKFFSDEEWELANDLQSRANARGMKL
                     VPERKIKNRAKFAQMLQDNVDYLEEIDYLDDKEIIFLWKISRKVGFLSNCLVDDIHKKS
                     PVPLTQTDIATLLKRTKNNVNPIVKSLVAKGILAVSKTGVQGNNSRANVYFMNPNILYA
                     GSKDDVNLTLQAMFSKVPKALKDMPVKLFELSCHSEEEEEKTN"
                     /name="hypothetical protein"
     ARG             complement(2073..2449)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="60.75"
                     /coverage="116.82"
ORIGIN
        1 gcttagtgcg tacatttccg ttctatcaat attttgtgta ataaaaaccc ttggggctag
       61 ccccaaaccc cagccagctc aacccaaaaa agcatatgaa aaccgtatca aatttaatac
      121 ggtaaaagaa taaaagactt aaaaacattt ttgcttagga aaggggtagc gtcacatgcc
      181 catcaactta agaacgaaaa caaaatgaac tttcgttcaa acaaactaaa aaacacgtaa
      241 tgtacttttg gtattgaaaa gtacacacac caaataaact ctaacgagtt tcattttaaa
      301 aattatgcag ctttcttttg ttttccaaac ccattatact catagacaac acccataata
      361 tcaaagatag ttttcttctc atatcgatga gatttccgtc cattcttctg taggaggtcg
      421 aacaaacgga ttaaaatctt tgttagattt tgggtgtttc tctgtatcgc ttgatataac
      481 agcgatacat gatcttgaat catcccaatg gctttatatt cacttaattc tcttttgttc
      541 ttttgcaaaa gaagtttacg catcttaaac atcgtagaag aacagataaa gatggcaata
      601 agtcttccat acacatgaca ttctaatcgc tcttttttga tagtttgcca atgatgaatt
      661 tgaaataagg atttccacgt tttaaaaata atttctattt gccagcgaag ggagtaaaag
      721 tcatgtattt gttccatcgg aacaactcct tcaggcgtat ttgagacata tatgttgata
      781 cccgttaatc gtttactctt ttctgaaaat gtaataccct ttttactttc agtgtaactt
      841 tgtttttttc ttctacgttc ctgtatctgt ttgtctgtta aacgatagat aattacacgt
      901 gtgaatagtt tttgattctt tccaatgtaa gcttctttta tttcgtatgt ttgaccgggt
      961 tttaaattgt tcataatatg ttctaaatca acttggatat actgtgattg cttttttacg
     1021 gttccgtttc gaaagtattc tggaaacgga ttttttacat acacggtatg gtttaatttt
     1081 aatctggata tataataagc acctcgttga tccatttgat ctaagtcttc taatgaaaaa
     1141 taacctaaat cacggataca taaatcgctt ggacgtaatg tgtctaagca ttctgttcca
     1201 aaggtcttat cattattttt tcctggacct atctgaaagt ttagaaactg tccgctatgt
     1261 aagtcatatt ctaattgaat cttgataccc gctgtttgtg cacaaccacc tgatccagga
     1321 tatacatggg ctaaatgttt tggtacttgg aaaatcgtcg catctaaaat acgaattcgt
     1381 tgaaaatacg tgagcgccgc gcttgaaatg gccgatgttt cacagatttt acttttccat
     1441 aatgccgaaa aaatatattt caaaaattca accgcttttt tatcaaagcg tttattgagt
     1501 ccttctggac tcataagagt gcctgtggcc gcatgtaatt gactgcatag tcgaacaagc
     1561 gagtcactcg ctgtacgttg acttacccaa atacatatgg tagctaattc atttccagaa
     1621 aactttcgtt ttctttttac aaaacctaat tcttttgcga gttcttccaa aagagaaggg
     1681 gttaaatgac gatacagttc ttcagaaaat agttgtaatt catcttgaat cgaaagattc
     1741 ataaaaaatg tcaccctttc tagctagctt tttagaaaga ataacgtttt ttgtcacttg
     1801 ggggtagtga aaattcttaa gttgatgggc atggggtagc gtcagggaaa tgcggattta
     1861 caacggtaag catttgatac aagtcttatg agacagtcag aagtcattta aaacgctaat
     1921 ttcattatgt ctcaataaac tctactctat tggaattgtg aagtgataca cttaaactaa
     1981 cgtacgcagg tttgttgaat aaggacttca aaaaataccc ttaattaagc taaaaagtcc
     2041 ttctctttat taagaaggac ttttttatgt gttattccat tgtctcattc attgtaatag
     2101 tttcgcttgc ttcatgacca gattctccat gtcctggtcc aaacaaattc aatattacaa
     2161 ctcctacaag aatcagtgtt attcctgcaa tgctagccat gttaagtttt tccttccaaa
     2221 ctaaaactga aattactgta gtcaaaacaa gtcctaaacc tgcccataat gcatatgcag
     2281 tgtttaaggg gattgttttt agcgacaagg ataaaaagaa aaaacaacca acgaaagcta
     2341 ttattacccc tattgtagga tagagttttg aaaagccttc agatgctttt aatagggaag
     2401 agccaataat ctcccccact attgcaagtg ctaaatatag atatgacatt attcgtcctc
     2461 ctgaatcatg tttattagtt catcaaaaac ttgtgttttt agttcttcat ttaatggtgc
     2521 cattccaaat atttctgaaa accaaagacc atctatggat aatcttgtaa ttgttatttt
     2581 gacaggatca attccatcag ttattaactt tgtatgcaaa atatcgtatc tctgccggaa
     2641 ttcatctaat aaatctggat taataaacat agtagccatt agtgcggaat ttagattatt
     2701 gttttctaaa tcggaaaatg tagatttaag ataagcccta ttccattttc ctattgcgtt
     2761 atcatcgtcg ttattaacta acgtattaat gcattcgaaa taattatttg tccaatcttc
     2821 caccattcct ttaattaatg cttccttgct aggaaaatgg tataataatc cacctttact
     2881 cacgccagct ctttgggcga ctgcctctaa tgtgagtttg acaactcctt cttcttttac
     2941 aatttcagat gcagcaatca atatattttt tttcttatta gcatgctttg aaatagacat
     3001 aataacctcc ataacttaga tacatataat ataccgtctg gacggttttt ttgtcaatcg
     3061 aattaaagct cttatttaat atcactcttt cacataaaaa taatccaata aattaatcta
     3121 aagattgtga agaaatgtac ttaaagtaaa gggagcttta gttcaacagt cgatttctta
     3181 aaatcggctt tatttatggg tgaaaattac attaccgttg taaatccgca ttttcaggac
     3241 tctaccccgg aaagaaaaac atttaaggtc gtaagacctt ttcaaaagca taaaaaaata
     3301 aggttttttc atcaaaatca gttgattaat ggtaaaattt gtatatattc atagtaattt
     3361 taaaaccaat tacttatttg tttaaacgct tgcggttaag caaatatgaa atacgaaatt
     3421 ttatactatg aatcttggga cgtgattcaa gtaatcgtgt attttacaaa actaaaaaat
     3481 tcacgtcata aaaatgaaag gggatcatta caatagcaaa gcaaaatgtt tttaagacgt
     3541 gggaaaaaat aatctaagaa aggagcaata taaagctttc ccaagtagtt ttataaatag
     3601 ctttaaaaga aaatagaatt cttgcttgca agaatggaaa aaaatattat aaaaaataaa
     3661 aatttaagga gatgacaaat caaaaaatac gaagggaaca agttttacaa ttaaatattt
     3721 ttacaactga ataaatgtaa atctgtaaca aaggttttta accacaaaac aaatcgcaag
     3781 aacagtaatt tctgggttac tcaaaaattc cagaccgcaa taccgttgta cgctcatttg
     3841 actatacatt ttggtgcaaa atgtatggga gagccttgga gtctatttat agacgccaat
     3901 cgcaacaaga tggcgtagct atcttgagcc tttgtctttt aatcttttga ccttgatttt
     3961 tgcttttggt cttttgattt tgactttgac tctagtgagc ttaaatcgcc cgaagggaga
     4021 tttcgcgatt gcttttgtgt gtttaatttg ggtggttcgg tctatttttt ttggtcttta
     4081 aaaaaaatag atcgaaccca cccaaatgag acctcgtttt tgttgtgcct tgatattgta
     4141 aggttcacgg gggttgggtt cttgctcttg attttgtata tagggggtga cgaaatgagc
     4201 aaaattacaa atgatattga tttaaaaatt gatgaagtct atgaaaaagt taaagatatg
     4261 aaaataactt ttgatgaaaa taatacaacc aaaaaacaat taactgattc aacaataaac
     4321 acatattgcg gaatcgtgaa atcatataac aattggttaa tacaaacgca tggaattagc
     4381 gtggataggg cgaaacctcg ccacgcaatg gaatatctcg aaacaaaaat tcaagccttt
     4441 gaaaaaggaa atgcttccgc attttctatg cgccaaatgg cgcatgcgtt tcattctttt
     4501 cgtgttgcga gtgctgaaac aggaacgttt aaacatacat gtaaggtcgc tgataaacga
     4561 gatatgttaa atacactcac agagcgtgga atcttccgta aatcggctga ttcgacggta
     4621 ttaaaggcaa atcattcaga ctatgaaaaa gttcaaaaag aactaaatgt agcaacgaca
     4681 agtaaacatt tgagagaaga tataaaaaca gtacacgaat tacagcggta cgctggaatg
     4741 cgtgttcacg aagccgtaaa actaaaagtg agtgatatag actttaagag tggcactgtc
     4801 tatataaaag gcaaaggcgg gcacgagaga acggttaaaa ttgaaagtaa agaattacta
     4861 gagaagttga aaaatttatg cactggaaaa agtgaaagaa gccaggtaat tattgctcga
     4921 aaacgtgata aaaatcaatt aacaaatgag gaatttggaa aacgtgcaaa agaagaaata
     4981 cgaggtgcag ccgtcaatgc tggggtggat cgtaacggaa aaagatacac aacacattca
     5041 ggtcgcaaag catatgcaca agcgcaaatg gataaataca aaagttacag taagaatcaa
     5101 ttaaaaaaag aagttgcaaa acgttgtcag gacaagaaaa caaagcaaaa atatgatgca
     5161 gttataaaaa atatccgtaa aaaaataacc gaaggttcga agaacgaagg gcgtaaactt
     5221 acaaataaag agatgtgcca gtttctagtt agcttggatt tgggtcacta tcgtttagat
     5281 gttgtacgat attatgctga ttattgatag gttttgacgg aaaatcggga agacgagtgc
     5341 ggagcacagt tggtactggc gcactggctt agaaacacaa gggctagagc ctcgttgtca
     5401 agccatcttt cccggcttta caatgagaaa gccttgtgtt agcctctcat ggcaatgcca
     5461 gtaccttgtg cggattcgag gaaatcgcca aaaaacgggt cgatgaacta agaagataca
     5521 cgagagtttt ccaaatcctc gccatttctc gctttttttc gaaaatcaaa aaaaaatgaa
     5581 acgcctatta aatttactat acagtaaaag tattttaaaa tacatttatt gtatagtaaa
     5641 tttatttttg agtaaaagta ttttaaagta attttaatag ttttacacaa tagtaaattt
     5701 atatacctaa attagattta cacaaaagta aaaatataga taaaaataca tgtattgtct
     5761 ccgcaattta tttttagtaa tcgcaaaaaa tcgttgattt aacaacgttt aagagatata
     5821 aaatacatgt acaaaatagt aaagtcatgg caataaatag atttacacaa aagtaaaagt
     5881 aatgacctaa aatggattta cataaaaata aatttaatgt ataataaatt taaagaagga
     5941 tatatataat tttgagtaaa tataattaat agtaaatgta aaatagatta aatatatttt
     6001 ttaataaatt tatttaaaat tatatttact tttcaataca tttactgtat attaaaaata
     6061 tagatggata catttaatta aaaatacatg tacttaattg taattaggag gaaaaaacaa
     6121 tgtcaaaaaa aatctcaatt gaagaaatga cgcaagaaat gaaggaaaaa ttcacgattt
     6181 cactggaaga tgcacataaa atccttttgg atcatggcgt aacgaaaagt acgaatacac
     6241 aagttacaat gcgttggatt cgtgatggga aaattgatgc attattgatt ggaaaaggtc
     6301 gtgtggagac tcgaaaatgg cttattaaca aggaaagtct aatggaattc gttgcattcg
     6361 agcgtttaac gttaggtgat ttcaaagcaa tgaaagaaga attagaaatg ttacgtgctt
     6421 ttaaattcga agtagagaaa aagccaaagc gtgctactgg tacacgtcaa aagaaagatg
     6481 cagcaacaaa tacttcaaaa gaaaagaaag aagtgccaac aaaataaaga ggtgaattgc
     6541 atgtcatctt ttagatggta tgaagaaaat gaagtgattg gaaaaactat aatagaagaa
     6601 gatgatttga ctttgctaga agctgggaaa atatatattg atgcaatatt ttgtgagaga
     6661 ataaggtttc cagttcaaaa aaacgaacag ccaattggat ttaattttgt ttatgaatat
     6721 aatgtaaaaa aatatttcgg attatatgac agaagtttca tgaagataat taaacaagaa
     6781 gatttagaag aatatcacat gagaatgata gattttcatg tgcacagatt atctttaggg
     6841 gaagaattag atgattgtcc aatcattggt atttgttatg gaagtggacc tttgataaca
     6901 ccgaggttgg aacaagttta tgatgtttcg aaaacaaaag catacagaaa gtatcgatta
     6961 gaaaaattat tcgattaggt aaagcatctt acaaaatttg agtttttaag ctagaattga
     7021 atagacaagc ataaaaaaaa gccgagtgtt ggagcactcg actttgggtg attcatcgta
     7081 atttatcacc aaaacgttga tttctagttt taattttaat gaattttcat ttaaaaatca
     7141 agggtaaaac cctttacgga taataaaaaa ataacggtga atctcctaga atgcttgttg
     7201 cattttcttc aggagaggag caaaatcaat ggcaattgat tttagtcaag cggatcgaaa
     7261 cgctagaaaa agagacttag aaaaagaaaa gtttttttca gatgaagaat gggaattagc
     7321 aaatgatttg caatcaagag ctaacgcgcg aggaatgaaa cttgttccag agcgtaaaat
     7381 caagaatcga gcaaaatttg ctcaaatgct tcaggataac gttgattatc tcgaagagat
     7441 tgattattta gatgataaag agattatatt tttatggaaa atcagtcgta aagttggttt
     7501 tttatcaaat tgtcttgtgg atgatattca taaaaaaagt ccagttcctt taacacaaac
     7561 agatatcgca actttgttga aaagaactaa aaataatgtc aatcctattg ttaaatcttt
     7621 agtagcaaaa ggcatactgg ctgtttcaaa aacaggtgtt caaggtaata attcgagagc
     7681 aaatgtgtat tttatgaatc cgaacatttt gtacgccggt agcaaagatg atgtcaattt
     7741 aacattacaa gcgatgtttt caaaagttcc taaagcatta aaggatatgc ctgtaaaatt
     7801 attcgaacta tcatgtcatt cagaagaaga agaagagaaa acaaattaat aataattaat
     7861 acatgtattt ttaatacatg tattttttta tctttcaatt atgtaaaata atgtaaaaaa
     7921 tgtaaaaaat ccggtgttct taaattcaag aactatggtt cttaaattca agaactacga
     7981 aaagtgcaac aaaccattga tattattgac tttttaatac attaacaatg ttgttctatc
     8041 tcactctcta ttaacgctaa ttttgagtaa agaaacgagt agtatttttt gcaaaaaccg
     8101 caaaaaatct ccactcttta tcttgactca aaattctccg caattggcat ggcttgtgcc
     8161 aagcgataaa ccaaaaaagt tatcactttt tcggattatc acttgaacaa tccattttaa
     8221 ttgctccgtc
//
