LOCUS       NZ_AP023003             8319 bp    DNA     circular CON 14-MAR-2024
DEFINITION  Bacillus cereus strain 30077 plasmid p30077_3, complete sequence.
ACCESSION   NZ_AP023003
VERSION     NZ_AP023003.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203343
            Sequence Read Archive: DRR206383, DRR206407
            Assembly: GCF_021655355.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8319)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 425x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655355.1-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 04:00:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,939
            CDSs (total)                      :: 5,787
            Genes (coding)                    :: 5,503
            CDSs (with protein)               :: 5,503
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 153 of 284
            Pseudo Genes (incomplete)         :: 140 of 284
            Pseudo Genes (internal stop)      :: 45 of 284
            Pseudo Genes (multiple problems)  :: 49 of 284
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023003.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8319
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="30077"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="p30077_3"
                     /geo_loc_name="Japan:Tottori"
                     /collection_date="2016"
     gene            join(8089..8319,1..42)
                     /locus_tag="L6491_RS29450"
     CDS             join(8089..8319,1..42)
                     /locus_tag="L6491_RS29450"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788729.1"
                     /translation="MSEVSMETVIKGKQQSELLKHLEKVGIELLGRRDELLEQWEKEGL
                     KEDSIFEDDLKFVEELMNRNDELMFDVKVELITTMDEIHHQKMGY"
                     /name="hypothetical protein"
     gene            47..298
                     /locus_tag="L6491_RS29455"
     CDS             47..298
                     /locus_tag="L6491_RS29455"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788731.1"
                     /translation="MLGKYDGLLEKERLLKRIDELSFALEASGDPMLEAMVKNSGNTDI
                     AVAKELITKGSELGMNEHVFKKALLAKVNELREEVTKL"
                     /name="hypothetical protein"
     gene            295..708
                     /locus_tag="L6491_RS29460"
                     /old_locus_tag="BC30077_p301"
     CDS             295..708
                     /locus_tag="L6491_RS29460"
                     /old_locus_tag="BC30077_p301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788733.1"
                     /translation="MKKDKQKDLPFNHKVVLSVLPLGRSKAITTKEIMARSGIKDKRAV
                     SAILSELVTVYKQPIATTSKKEFKGVFIVQDVIDYQIGKEGLVSRKRHIDKRDSAYTEA
                     CLEIFKGTVTLDESKNKVEGSVRACKQSASSSM"
                     /name="hypothetical protein"
     gene            678..1385
                     /locus_tag="L6491_RS29465"
                     /old_locus_tag="BC30077_p302"
     CDS             678..1385
                     /locus_tag="L6491_RS29465"
                     /old_locus_tag="BC30077_p302"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788735.1"
                     /translation="MQTISKFFNVDNESKLNELTIAKGELNSKESELQSKLSRVERALE
                     LAQTDLMIDDSTANKKVVAKYEEAQEKFQSELIATQEELNKVSDEIAAIVKEELHAELV
                     DAAKQDVMLIELPEKCRKAKKVINRDLYRATSFEDEGRFSRKYLHVSATVIDDARTFAD
                     IHRFADKPNLTRIGYQRGELIRWGGDNNLFKLNDEYETIYEEEAQKIKDKTDKEYEQFM
                     KAFNKYFAVSDSH"
                     /name="hypothetical protein"
     gene            1439..1864
                     /locus_tag="L6491_RS29470"
                     /old_locus_tag="BC30077_p303"
     CDS             1439..1864
                     /locus_tag="L6491_RS29470"
                     /old_locus_tag="BC30077_p303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788712.1"
                     /translation="MSKDTLKNIFHVYCFYTVRLQGDTEPRMRRNKLNYDNPIQNYYES
                     FIDCLRGFCEVRSDVSLYSFYQFVVDAVNSLNKQERKLIYERYLYKDHYKSDRQHYLAM
                     DITPQNYKKQMDPVRRKLIKNLGIEDLQLNIPDWMKR"
                     /name="hypothetical protein"
     gene            1995..2243
                     /locus_tag="L6491_RS29475"
                     /old_locus_tag="BC30077_p304"
     CDS             1995..2243
                     /locus_tag="L6491_RS29475"
                     /old_locus_tag="BC30077_p304"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_236788716.1"
                     /translation="MKSLYRILELDKDEVSSSEKLILLTIAIYSDNKFIPFSITELENY
                     TNLSRATVTRLVKQLEEKGFVEVQRNGTATNNYKVTL"
                     /name="helix-turn-helix domain-containing protein"
     gene            2320..2496
                     /locus_tag="L6491_RS29480"
                     /old_locus_tag="BC30077_p305"
     CDS             2320..2496
                     /locus_tag="L6491_RS29480"
                     /old_locus_tag="BC30077_p305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176543923.1"
                     /translation="MLPQKEQLGHLAEKTELVKDLAWIAHDLLTEEDYTKENAAEALIK
                     VINRELSYVSKVR"
                     /name="hypothetical protein"
     gene            2538..2744
                     /locus_tag="L6491_RS29485"
                     /old_locus_tag="BC30077_p306"
     CDS             2538..2744
                     /locus_tag="L6491_RS29485"
                     /old_locus_tag="BC30077_p306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788718.1"
                     /translation="MANTVAKDKFEIVEKFVNACMLKGVSEDKSFKLVEELCTLIQQKR
                     HDEWFGLVDEALKEAGHEEGVTL"
                     /name="hypothetical protein"
     gene            2741..2872
                     /locus_tag="L6491_RS29705"
                     /old_locus_tag="BC30077_p307"
     CDS             2741..2872
                     /locus_tag="L6491_RS29705"
                     /old_locus_tag="BC30077_p307"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272880692.1"
                     /translation="MSEVEMVLHEFEELLHKIISAAQHGDEQAMNHLDIVKAWLKDN"
                     /name="hypothetical protein"
     gene            2949..3188
                     /locus_tag="L6491_RS29490"
                     /old_locus_tag="BC30077_p308"
     CDS             2949..3188
                     /locus_tag="L6491_RS29490"
                     /old_locus_tag="BC30077_p308"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788720.1"
                     /translation="MAEEHRYHVTFYFSNGKEFDGKVTSKYNKEDYLEGLEDLFLKEKT
                     LLIDHLGMLIQTKYITHVKVIEVGSKIGADKKDT"
                     /name="hypothetical protein"
     gene            3249..3785
                     /locus_tag="L6491_RS29495"
                     /old_locus_tag="BC30077_p309"
     CDS             3249..3785
                     /locus_tag="L6491_RS29495"
                     /old_locus_tag="BC30077_p309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3967 domain-containing protein"
                     /protein_id="WP_236788722.1"
                     /translation="MTDKTDSSQSIYISKDVAMMLKIQESTLRKYCIMLEEHGYKFHKN
                     EHGHRGFLDNDVITLRKLIEIKSHPDMTLKQACNAIMTWVKEKDMSEVDTNVITENEQH
                     DERYSELKEMIQQQNELLKKMAKKLDDQQRYIDEKLEKRDQQLMGAIREMQEEKRVLLE
                     TASASQEKKWYKFWK"
                     /name="DUF3967 domain-containing protein"
     gene            4409..5008
                     /locus_tag="L6491_RS29500"
                     /old_locus_tag="BC30077_p310"
     CDS             4409..5008
                     /locus_tag="L6491_RS29500"
                     /old_locus_tag="BC30077_p310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562260.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_236788723.1"
                     /translation="MNKRFAYIRVSDKTQNENRQLDEMLQQEIEERDIFIDKASGKDFN
                     RPKYQALKQMLRAGDILYIKSIDRFGRNKDDILKEWNEITKEIGADIVVLDMPLLDTRQ
                     YKDVLGSFVSDLVLQILSFFAEEERRKIKERQREGIDAAQRRGAKFGRPAATFPPGFEE
                     VYKRWKDGEIKAIEAMEELELKRTTFYKLVNSFENK"
                     /name="recombinase family protein"
     gene            5764..5961
                     /locus_tag="L6491_RS29505"
                     /old_locus_tag="BC30077_p311"
     CDS             5764..5961
                     /locus_tag="L6491_RS29505"
                     /old_locus_tag="BC30077_p311"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788725.1"
                     /translation="MIGKDMITLDNPKKEYFQQYAQNNKEQINANARRRRQQQKEKVEK
                     YDRLVAALRSRGISVEELLK"
                     /name="hypothetical protein"
     gene            6384..7484
                     /locus_tag="L6491_RS29510"
                     /old_locus_tag="BC30077_p312"
     CDS             6384..7484
                     /locus_tag="L6491_RS29510"
                     /old_locus_tag="BC30077_p312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205124.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_098492605.1"
                     /translation="MNHEEFINSTEIDDKFLKQIIDNREFNDVIVSYYERLHKESRDDG
                     MKKKQENVKGCNDFWIIDKYHEHRLKEFKRTNLCKDKFCNNCKKVKQASRMGKFIPLIQ
                     PYAENMYQLTLTIPNVKGDELKDTLSKMQKAFARLIEYFKGKKKINGIDFEVLQYEGAI
                     RSLEITFNGNEYHPHYHALIVLKVNKFHDCMIRKHKNVYSIDFTGKREARLFSDIEVLI
                     QKVWYLIINGKRITETAIQSLKKGYSCQLDKFSENDFVEIFKYMTKATTEDGKTLSYKQ
                     FKTLYYALFKVRQIQGYGIFYNLKDDEEINFDEVNEAYAEFIEELQTKEKPVTVSEAPE
                     QVRKESDYTVFSRKTFQKYWKQVMSE"
                     /name="protein rep"
     gene            7952..8101
                     /locus_tag="L6491_RS29515"
                     /old_locus_tag="BC30077_p313"
     CDS             7952..8101
                     /locus_tag="L6491_RS29515"
                     /old_locus_tag="BC30077_p313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788727.1"
                     /translation="MEREKCSLKEAKNAYNRKWRAANRDKVKLYNDRYWEKKAKEMEEQ
                     ACQK"
                     /name="hypothetical protein"
ORIGIN
        1 acaactatgg atgagattca tcatcagaaa atggggtatt gataggatgt taggaaagta
       61 tgatggatta ttagaaaaag aacggttact taaacgaatt gatgaactgt cattcgcgtt
      121 agaagcttca ggtgacccta tgttggaagc gatggttaaa aatagtggca atacagacat
      181 cgcggttgca aaagagttga ttacaaaagg gagtgaatta ggtatgaatg agcacgtatt
      241 taaaaaagcg ttgctagcaa aagtaaatga acttcgtgaa gaggtaacga aactatgaaa
      301 aaagacaaac aaaaagacct cccttttaat cacaaagtag tattgagcgt actaccttta
      361 ggaagatcta aagcaataac aactaaagaa attatggcac gtagtggaat aaaagacaag
      421 cgtgcagtat ctgctatttt aagtgaatta gtgactgtat ataaacaacc tattgctaca
      481 acgtctaaaa aagaatttaa gggcgttttt attgtacagg atgtaataga ttatcagatt
      541 ggtaaagaag gccttgtgag cagaaaaagg catatagata agcgtgattc agcttataca
      601 gaagcgtgct tggaaatatt caaaggtaca gtgacattag atgagtcaaa aaacaaagtg
      661 gaaggaagtg ttcgtgcatg caaacaatca gcaagttctt caatgtagat aacgagagta
      721 agttaaacga attaacaata gcaaaagggg aattgaattc caaagaatca gaattacaaa
      781 gtaagctatc tcgagtagaa agagctctgg aactggcaca aactgactta atgattgatg
      841 atagtacagc taataaaaag gttgttgcaa aatatgaaga agctcaagag aagttccaat
      901 cagagttaat agctactcaa gaagaactta acaaggtttc tgatgagatt gcagctatcg
      961 ttaaggaaga attgcatgct gaattagtag atgccgctaa acaggacgtt atgctaatcg
     1021 aactacctga aaagtgcaga aaagcgaaaa aagtaattaa cagggatctt tatagggcga
     1081 cgtcctttga agatgaaggt cgatttagta gaaagtattt gcatgttagt gctacagtga
     1141 tcgacgacgc tcgtactttc gccgatatac atcgttttgc tgacaaacca aatctaacac
     1201 gaatagggta tcaacgcggt gaattgatac gttggggtgg cgataataac ctgttcaaat
     1261 tgaatgatga atatgaaacg atatatgaag aagaagcaca gaaaatcaaa gacaaaacgg
     1321 ataaagagta tgagcagttc atgaaggctt tcaataagta tttcgctgtg tcggattctc
     1381 attaatcaaa atacataagt cgcctaaggg cggctttttt ttattaggag ggattttcat
     1441 gagtaaagat acattaaaaa acatctttca cgtgtattgt ttttatacag tacgtttgca
     1501 aggcgataca gaaccacgta tgagacgaaa caagctaaat tatgataatc ctatacaaaa
     1561 ctattatgaa agttttatag actgtctgag aggattctgt gaggttagga gcgacgtttc
     1621 attgtattcg ttctatcaat ttgttgtaga tgctgttaat tcgttaaata agcaggaaag
     1681 aaagctgatc tatgagcggt atttatataa agatcactac aaatcagata ggcaacatta
     1741 ccttgcgatg gatataacac cacagaatta taaaaaacag atggatccgg taagaaggaa
     1801 gttaattaag aacctcggaa tagaagattt acagttgaat attccagatt ggatgaaacg
     1861 ataacgggtt aaattacatg tatggttata ttatttttaa attttatttt gattctattg
     1921 cattttacta tactcattgt tatactagat aatgtaaaca acatgttgat ttataggagg
     1981 ttactagttt aattatgaaa tcactatatc gtattcttga attggacaaa gatgaagttt
     2041 cttcttctga aaaactaatc ttgctaacga tagcgatcta ttcagacaac aagtttatcc
     2101 cattctctat tacagaattg gagaattaca cgaatttgag tagagctacg gttacaaggc
     2161 tcgtaaaaca gttagaagaa aagggattcg tcgaggttca aaggaatgga acagcaacaa
     2221 acaattacaa agtaacactg taaaaacaaa gaaagagcag gagattgcgg ctcctactct
     2281 ttcaaaacac attatccctt atggaaagga tgtattagta tgttaccaca aaaagaacag
     2341 ttaggacatc ttgctgagaa aactgaatta gttaaggatt tagcttggat tgcacatgat
     2401 ttacttaccg aggaagatta caccaaagag aatgcagcgg aagcgttaat taaggtcatt
     2461 aatagggaat tatcatacgt ttccaaagtg cgttaaacgg gattatatgt aaataaaaca
     2521 attcggaggt aatattcatg gcaaatacag ttgctaaaga taaatttgag atcgtagaaa
     2581 agtttgtgaa tgcttgtatg ttaaaaggtg tttcggaaga taaatcattc aaattagtag
     2641 aggaattatg tacacttatt caacaaaaac gtcatgatga atggtttgga ttagtagatg
     2701 aagcacttaa agaagcggga catgaagaag gtgtaacgtt atgagtgaag tagaaatggt
     2761 tttacacgaa ttcgaagaac tattacataa aatcatatca gcagcgcaac atggcgatga
     2821 acaagcgatg aatcatttag atatagtaaa agcatggtta aaggacaatt aaggcgtggt
     2881 gatccacgtc ttttttttat actgtcttgg ggaatcctac tgatatattt tgaaagtagg
     2941 tggactaaat ggctgaagaa catagatacc atgtaacttt ttatttttct aacggaaaag
     3001 aatttgatgg taaggtaaca agcaaataca acaaagaaga ttatttagaa gggttagaag
     3061 atctattcct taaagaaaag acattactga ttgatcatct aggaatgtta atacaaacca
     3121 aatatattac acatgtcaaa gttatcgaag ttggatctaa aataggagca gacaaaaaag
     3181 atacgtgaat tattaaggaa aaggatatat cattgttata acgaatgttt tggtagaggt
     3241 gaagcgttat gacagataaa acagacagct ctcaatccat atatataagc aaagacgtag
     3301 cgatgatgct taaaattcaa gaatcgactt tacgtaaata ttgtatcatg cttgaggaac
     3361 acggctacaa atttcataag aatgagcacg gccatagagg attccttgat aacgatgtta
     3421 taacgttaag aaaattaatc gagattaaat cacatcctga tatgacatta aaacaggcct
     3481 gtaacgcaat tatgacgtgg gttaaagaga aagatatgtc agaggttgat acgaatgtta
     3541 taacggaaaa tgagcaacat gacgagcgat atagtgagct taaagaaatg attcagcagc
     3601 agaatgaatt attaaagaaa atggcgaaga aattagatga tcagcagagg tatattgatg
     3661 aaaagttaga aaaacgagat cagcagttaa tgggagctat ccgagaaatg caagaagaaa
     3721 aacgagtttt gttagaaacg gcttccgcta gtcaagaaaa gaaatggtac aagttttgga
     3781 agtaggattc cctaactatg gggagtcctt ttttatttta aagtgagttt ggatcactta
     3841 gaggtagatt aaacagaaag ccctgcaggt aacatcctca gaaacgcaca agaaaagtgg
     3901 tgcgtataac tgggcactcg cttttttctc gtgaataaga tctcattgaa tggagtaatt
     3961 ttgtatacta ctccgttgga atatagaacg ttttacggag cgttccaata tcgttacctt
     4021 tgcaaaacaa acacgaatac cacgttactt taatccgcac acaaggcacc ctttttatgc
     4081 agtactcatt gtagtattgc acccctttta atgttgtact gcaaaacaaa cacaactcaa
     4141 ctaataaaga acaaagcaca tataccggta attgaatggt aacgggtgtt aaggaatgat
     4201 aaggagtatt agtgcaaaat gaatgtagta gtattttagt aatttgcaaa acaaacgaac
     4261 tcaattaatg tattcgtaaa agtacacatt tacgaataag aaaaagaata agaaaaagat
     4321 attgttttgt gttcgtataa gttgtatgat tagtttacaa acattcgtaa attgaaaaac
     4381 acttttacga acaaagggga agaggaaaat gaacaaacgt ttcgcttata tccgtgtatc
     4441 agataaaact caaaacgaaa atagacagtt ggacgagatg ttgcagcagg agattgaaga
     4501 aagagatatc ttcattgaca aggcaagcgg taaagatttt aatcgtccta aataccaggc
     4561 tttaaaacag atgctacgtg ccggagatat actgtatatc aaatctattg atcgttttgg
     4621 tagaaataaa gatgatattt taaaagagtg gaatgaaata acgaaggaga tcggtgcgga
     4681 tatcgttgta ttggacatgc ctttattaga tacaagacaa tataaagatg tattaggctc
     4741 gtttgtaagt gatttggtac tacagatact atcattcttt gcagaagaag aaagacggaa
     4801 aatcaaagag agacaacgtg agggcattga tgccgcacaa agacgaggtg cgaagttcgg
     4861 aagaccggct gctacattcc cgccaggatt cgaagaagtg tacaagcgtt ggaaagatgg
     4921 agaaattaaa gcaatagaag ctatggaaga attagagtta aaacggacta ctttctataa
     4981 actagtaaat tcattcgaaa ataagtaaaa aaagtaataa taatattaaa ctttttacaa
     5041 aattaagtcg aaaaagcaaa gttaaaaaaa gacaaacata ccgataacaa gcattgatat
     5101 tacacataat tttatgcctt tcatgtcgaa aagtaaaagt taaaaaagta ataaaaaagc
     5161 aaagtataaa aagtatactt ttaagtttgc ttgtttctat gaaaatccgt tggtataata
     5221 gttttaacgt gtttgagtgt ttcaaacgaa aagaaaaaag ccgagttggg gaactcgact
     5281 taagtctttc taaaagtatg gggatacttt tagattagat tttgtgtctc gacaacactt
     5341 tcgactaatt agcactacca atgcttatta gtcattgatg attttacgaa tcatcttaac
     5401 gattattttt ttatggattg acacctattt tatcgacctt tgacaatcaa ataattaggt
     5461 accatttata tctaagattg aattaacaac cttatttaac gtatgaaaaa gtactgtagg
     5521 tcaagttgta aactataaaa agtttcttct taggaaacct taagagaaaa aaatatagat
     5581 acaatttgta tcgctttaat acgaaaaaaa gttctcgttc agcgaacttc ctatgtgaca
     5641 agtttacaat gtatttttcg ttttgacaat actttaagta aactcatagt ttttacatac
     5701 attgaaaggt acctctacta tttgaggtgc cttttttatt tatttttgaa aggatgatag
     5761 aaaatgatag ggaaagatat gatcacatta gacaacccaa agaaagagta ttttcagcaa
     5821 tatgcacaaa ataataaaga acaaattaac gccaatgcaa gaagacgaag acaacaacaa
     5881 aaagaaaagg tagagaagta tgatagattg gttgcggcgt tacgatctag aggaattagt
     5941 gtagaagaat tattgaaata agatggacaa gctgtattaa ggccggacta agacaccagg
     6001 agaagatttg tatacggtct aggttattcg tggggcttat gctacagctg tcattgtcta
     6061 gcggtatcgc agtgcgtaag cacgagaata aggagcagac atacaccaca agccccgaac
     6121 actttcatgc tctccaaaac aacatgtttt ggggatacct agtgtaacgt ggccgagtgc
     6181 aacggaaggt tttaatgttt cgtttaaaaa atgagtttct tctattactt gatatattag
     6241 aaataagtcg gctatttctc tagttttcta gtaatgacaa attgtcgata accatagtca
     6301 tatcaagggt ttaagctatt cgacttattg ctaataaggc ttaaaaagcc ttccaaaaat
     6361 tccactagaa atgaggtgag aaattgaatc atgaggaatt tataaattca actgaaatag
     6421 atgataaatt tttaaagcaa atcattgata atagggaatt taatgacgtt atagtctcgt
     6481 attatgaacg actacataaa gaatcacgcg atgatggaat gaagaagaag caggaaaacg
     6541 ttaaaggttg caatgatttt tggattatag acaaatatca tgaacataga ttaaaagagt
     6601 ttaaacgaac taatctttgc aaggacaagt tttgtaacaa ctgtaaaaag gttaaacaag
     6661 cttcacgcat gggtaaattc ataccactta ttcagcctta cgcggaaaat atgtatcaat
     6721 taacgctaac tattcctaat gttaaaggcg atgaattgaa agatacacta agcaagatgc
     6781 aaaaagcttt tgctcgttta attgagtatt tcaaaggcaa aaagaaaata aacggaattg
     6841 attttgaagt tttacaatac gaaggcgcta tacgatcttt agaaataaca tttaacggta
     6901 atgagtatca tccacattat catgctctta ttgttttaaa ggttaataaa ttccatgatt
     6961 gcatgataag aaaacataaa aatgtttatt caatagactt cactggaaaa cgtgaggcgc
     7021 gtttatttag tgatatagag gttttgatac agaaggtttg gtacttgatt ataaacggta
     7081 aacggataac agaaacagcg atacagtcac ttaaaaaagg ttattcatgt cagttggaca
     7141 agtttagcga aaacgatttt gtagagatat ttaagtacat gacaaaagca acaacggaag
     7201 acggtaaaac attgagttat aagcaattca aaacgttgta ttatgcgtta tttaaagtta
     7261 gacaaataca agggtatggc atcttttata atttgaagga tgatgaagaa attaattttg
     7321 atgaagttaa tgaagcgtat gcggaattca tagaggaatt acagacaaaa gaaaaacctg
     7381 ttacggtatc agaagcacca gaacaggtca gaaaagaaag tgactataca gtgtttagtc
     7441 gaaaaacgtt ccaaaaatat tggaagcaag tcatgagcga atagccttac tcattccggc
     7501 aagtttgata aaaggctatc tttaccacca ttaatcaaat aacggagggt tttctaatat
     7561 cattatacat aattttaatt cgtatgatat taggaaattc cttctatatc gttaattagt
     7621 agagactatg aattatttcg tagtcttttt ttattggaaa ggaatgattg ttgttatatt
     7681 tagaggttta taacaggaaa aaggcgcctg atctcgtttg aatcagaacg aaatcaagcg
     7741 cctttgggcg ataattaacc aagaacaagt taattaaata aatctaaaag tttttggaag
     7801 tacttatatt atatcgtata tggagttccc ggacagtaat tttagcctaa atttgcgtga
     7861 agcaaatcat taatcatacc acgtaaacta tatattcgta aataggtaaa tgttaaaata
     7921 agagtaagaa gaatgaaaag aaggtggaaa agtggaaaga gaaaagtgtt ctttgaaaga
     7981 agctaaaaac gcttataacc gaaaatggag agcagcgaat cgcgataaag tgaagctgta
     8041 taacgataga tactgggaaa agaaagctaa agaaatggag gaacaagcat gtcagaagta
     8101 agtatggaaa ctgttattaa aggaaagcag cagagtgaac tgttgaagca tttagaaaaa
     8161 gtaggtattg aattactggg gcgtagagat gaattgttag agcagtggga aaaagaagga
     8221 cttaaagaag acagcatttt tgaagatgat cttaaattcg ttgaagaatt aatgaaccga
     8281 aatgacgaat taatgtttga tgtaaaagtg gaattaatt
//
