LOCUS       NZ_AP022999             5049 bp    DNA     circular CON 14-MAR-2024
DEFINITION  Bacillus cereus strain 30075 plasmid p30075_5, complete sequence.
ACCESSION   NZ_AP022999
VERSION     NZ_AP022999.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203342
            Sequence Read Archive: DRR206382, DRR206406
            Assembly: GCF_021655335.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 424x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655335.1-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 03:49:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,110
            CDSs (total)                      :: 5,958
            Genes (coding)                    :: 5,598
            CDSs (with protein)               :: 5,598
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 216 of 360
            Pseudo Genes (incomplete)         :: 158 of 360
            Pseudo Genes (internal stop)      :: 51 of 360
            Pseudo Genes (multiple problems)  :: 59 of 360
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022999.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="30075"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="p30075_5"
                     /geo_loc_name="Japan:Tottori"
                     /collection_date="2016"
     gene            428..580
                     /locus_tag="L6490_RS30335"
                     /old_locus_tag="BC30075_p51"
     CDS             428..580
                     /locus_tag="L6490_RS30335"
                     /old_locus_tag="BC30075_p51"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796023.1"
                     /translation="MPKVVSSKEKMQPKTFKLHPRTIEQIKELAEKKQMTQAEVIDKAL
                     KLLAR"
                     /name="hypothetical protein"
     gene            673..1395
                     /locus_tag="L6490_RS30340"
                     /old_locus_tag="BC30075_p52"
     CDS             673..1395
                     /locus_tag="L6490_RS30340"
                     /old_locus_tag="BC30075_p52"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_236796021.1"
                     /translation="MKKAFAENYINPVVERFSELDTPNGGFYTSIPQAVQYCWFLDDKE
                     LSVLLALSTYADENGRCKVYQRTLARKVKIKSTTTLRTKLKSLVKKRFILQVNTDGESS
                     IYQIKDYSTNPYILLSEAIEKVEQVAKKKYGVSVSKIAETMDELIASKIDYMAMITKIE
                     ESFNGYDVALKSVQGYVNEALGLEMDISLQTKKPRRERGFKMSKVIKPKQPKRNREFII
                     SEGESEAPVKREGLRLRL"
                     /name="helix-turn-helix domain-containing protein"
     gene            1598..2443
                     /locus_tag="L6490_RS30345"
                     /old_locus_tag="BC30075_p53"
     CDS             1598..2443
                     /locus_tag="L6490_RS30345"
                     /old_locus_tag="BC30075_p53"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_236796018.1"
                     /translation="METDISIDRLTLVGYDTGMIEGYLKYSEYISFVGFSKNYGFRHSY
                     QGIKGELLEIGEKGKVRLDFNPNTANMEQVRDVVSRLKYIYLTRLDIAIDYFGVDLSEV
                     EWTSPKRRKQNLWLDPEGQQETRYIGAPSSERRYRIYNKKLERLEKKEELDPRAVNGQW
                     RVEVQQRFKESNNILDPIDYFTGDLFDIRPYRKVVDLSHIEKPMERIIIAGLMARPSEL
                     KNLSKNARGKYKKMIQEAREKSGVAINPEPIEVYTKEKDLLAEQLTSLFAHCAKSVAQV
                     "
                     /name="replication initiation factor domain-containing
                     protein"
     gene            2861..3184
                     /locus_tag="L6490_RS30350"
                     /old_locus_tag="BC30075_p54"
     CDS             2861..3184
                     /locus_tag="L6490_RS30350"
                     /old_locus_tag="BC30075_p54"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796016.1"
                     /translation="MMMNLLSLQQEANSVIQDLNGKDISSMSLIQKANYSNRIVKVRMS
                     LEAYRNTPGVNEFINALDHLRFKLAEVQQQETRQQANKRRAEIIQNGIKETPYPTMPMF
                     LNN"
                     /name="hypothetical protein"
     gene            3196..3534
                     /locus_tag="L6490_RS30355"
                     /old_locus_tag="BC30075_p55"
     CDS             3196..3534
                     /locus_tag="L6490_RS30355"
                     /old_locus_tag="BC30075_p55"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="WP_236796014.1"
                     /translation="MSDNVNFPLRIPRELRDKFKKVSQKTGTSMNSSICQLIAEHIYKM
                     EQLGLKDIEEPNVIQVTPVKEETKAFIKKAIKEADEAKELAETKTKKRLRKSPKKKIGG
                     ETDGSKKD"
                     /name="Arc family DNA-binding protein"
     gene            3515..3754
                     /locus_tag="L6490_RS30360"
                     /old_locus_tag="BC30075_p56"
     CDS             3515..3754
                     /locus_tag="L6490_RS30360"
                     /old_locus_tag="BC30075_p56"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796012.1"
                     /translation="MEVKKIKIKPKKIVRNEENHKLEFDKVRLSIEIPISIRDNFKEAV
                     EKNGKTMKEVLEAYMQRYSVKELLTDLKEETIDN"
                     /name="hypothetical protein"
     gene            4238..4438
                     /locus_tag="L6490_RS30365"
                     /old_locus_tag="BC30075_p57"
     CDS             4238..4438
                     /locus_tag="L6490_RS30365"
                     /old_locus_tag="BC30075_p57"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796011.1"
                     /translation="MDIEAKKEAFVTFLVEKVLFQTFAERTTSLEEKEVYEKFIKLYDQ
                     ELNRLNFELTIASVNRKRANL"
                     /name="hypothetical protein"
     gene            complement(4462..4608)
                     /locus_tag="L6490_RS30370"
                     /old_locus_tag="BC30075_p58"
     CDS             complement(4462..4608)
                     /locus_tag="L6490_RS30370"
                     /old_locus_tag="BC30075_p58"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796009.1"
                     /translation="MKKTKKQAKKQPKKQPKKQMKTAMKIVGITKDVVNFVGNLIRIYT
                     FFF"
                     /name="hypothetical protein"
ORIGIN
        1 aaagattgaa aaaggtatct aatttagtag cccaaatagt agctcaaaag tgccattact
       61 ctttctatac aaaacaataa cgatttttca tttgaaaata gtctcccaaa aagtttgggt
      121 tttgttcgga tgattaagaa ctatgtattc tagttcttgc tctgattttt tataactata
      181 gggatgttcg acacgtcgaa aaacccgctt ctctaacagc gggttagcgt cctctttcga
      241 gggattcacc atatcaaaat gttttttgtt ttaggttttc tcctttcttt tttgatcaaa
      301 aaaatgtcct tattagtttg ttctattttt ttatgtgttt tttgactgtt tttgaagtgg
      361 acaaatagaa tcgttgatgt taagctgttt ttagggagta cctatcattg tgttgaggag
      421 tgatattatg ccgaaagtag ttagttctaa ggaaaagatg caacccaaaa catttaaatt
      481 gcatcctagg acgattgaac aaataaagga attagctgaa aagaagcaaa tgacgcaagc
      541 tgaagtgatt gataaggcgt taaagttatt agcacgataa aaaaatatat atcccatcgc
      601 aaataacgta gcatactaac atagcaaaca agataacaag gagggaaaat taaaaggaaa
      661 aggtgatctg taatgaaaaa ggcatttgca gagaattata ttaatcctgt tgtggaacgt
      721 tttagtgaat tagatactcc aaatggcggt ttctatactt ccattccaca agcagttcaa
      781 tactgttggt ttttagatga taaagagtta agtgtattat tagcactatc gacctatgca
      841 gacgaaaatg gacgttgcaa ggtatatcaa cgaacgttag ctaggaaagt taagattaaa
      901 agtacaacaa cattacgaac taaattgaaa agtttagtga agaagcgttt tatattgcag
      961 gttaatacag atggagaatc tagtatttat cagattaaag attattctac caatccttat
     1021 attcttctta gtgaggctat tgaaaaagtc gaacaggtag ctaaaaagaa atatggtgtt
     1081 tcagtatcta aaatagctga aacgatggat gaattaattg ctagtaagat agattatatg
     1141 gctatgataa ctaagataga ggaaagtttt aatggttatg atgtagcttt aaaatctgta
     1201 caaggttatg taaatgaggc tttaggatta gaaatggata tttctttaca aacgaaaaaa
     1261 cctcgacggg aacgaggttt taagatgagt aaagttatta aacctaagca acctaaacgt
     1321 aacagagaat ttattatttc agagggtgaa tctgaagctc ctgtgaaacg ggaaggtctt
     1381 agattacgac tttgaataag gtaattatat attaccttat aggacttgaa tacctagtca
     1441 aaaattgatt accccttaca caatttttgt gtacctctta atcaaaattt gattactact
     1501 aaatataaaa tactaaaaag aaattaatat atatatctat caaagaaatt actgaagcat
     1561 actaagatag caaataaaac ggaaatgagt gtttaggatg gaaacagata ttagtataga
     1621 ccgcctaact ctcgttggat acgatactgg aatgatagaa gggtatctta aatactcgga
     1681 atatatttca ttcgtggggt tttctaagaa ttatggtttt cgtcattcat accaagggat
     1741 aaaaggagaa ttgcttgaga taggcgagaa agggaaggta cgtttagatt ttaaccctaa
     1801 taccgcaaat atggagcagg taagagacgt cgtttctcga ctgaaataca tctatttaac
     1861 gcgattagat atagcgatag attattttgg tgtagattta tctgaggttg agtggacaag
     1921 ccctaaacga agaaaacaaa acttatggtt agacccagaa gggcaacaag aaacgagata
     1981 cataggtgcg ccatcaagtg agagaaggta tcgtatttat aacaagaagt tagaacggtt
     2041 agaaaagaaa gaagagttag accctagagc agttaatgga cagtggcgtg ttgaggtaca
     2101 acaacgtttt aaagagtcca ataacatatt agaccctatt gattatttta caggtgatct
     2161 attcgatatt agaccatatc gaaaggtggt tgatttatct catatagaaa agcctatgga
     2221 acgaattata atagcgggat taatggcaag acctagtgaa cttaagaatc ttagtaagaa
     2281 cgctagaggt aagtataaga agatgataca agaggctagg gaaaaaagcg gtgtagcgat
     2341 aaatcctgaa ccaatagaag tttatacaaa agaaaaagac ttgttagctg agcaactaac
     2401 aagcctgttc gcccactgtg ctaagagcgt agcacaagtg taaactttaa aacttataac
     2461 aagtaaatat ctaatgttag tatagcaaaa gaagagaggg aattcacctc tctttttctt
     2521 tgttttaagg ggattcgtta agaatatatg tatttccttt aagtttattg cgttagctcg
     2581 tgctttagga ttaatagttg tctatttagg ctattgtttg aaaaacaccc cacccaccgc
     2641 ttattaggtt ctgtatctga aaatgcgctc ctacaccctc tcgattttgt taatttttat
     2701 ataggtaagg tgacaattta aaaataaatt ttaaattttt taagtaaccc agtatacaga
     2761 tttttggtta agagttacga tgtttttata aatgactatc atattgataa tcaatttaat
     2821 agtcgtattg attatcacaa cagaaaagag ggaaaatatt atgatgatga atttgttaag
     2881 tttacaacag gaagctaaca gtgtgattca agatttaaac ggtaaggata tttcttcaat
     2941 gagtttgatc cagaaggcta attattctaa tcgaatagtg aaagttagaa tgagccttga
     3001 ggcatataga aatactccgg gagtaaatga atttataaat gcattggacc acttaagatt
     3061 taagttagct gaagtacaac aacaggaaac acgacaacag gctaataaaa gaagagctga
     3121 aataattcaa aatggaataa aagaaacacc gtacccaacg atgccaatgt ttctaaataa
     3181 ttaggaggat aggatatgag cgataatgtt aattttccat tgagaatacc tcgtgaattg
     3241 cgggataagt ttaaaaaggt ttcacagaag actgggacga gtatgaactc atcaatttgc
     3301 caactaatag cggaacacat ttataaaatg gagcaattag gattgaagga tatagaagaa
     3361 cctaatgtaa ttcaagttac gccggttaaa gaagaaacaa aggcgtttat aaagaaggct
     3421 ataaaagaag ctgatgaagc taaggaatta gctgaaacta agacgaaaaa aagattaaga
     3481 aagtcgccca agaagaaaat aggcggtgaa acagatggaa gtaaaaaaga ttaagatcaa
     3541 acctaaaaaa atagtgcgta atgaagaaaa tcataaatta gaatttgata aggtaagatt
     3601 aagcattgaa atccctattt ctattagaga taactttaaa gaagcggtag aaaagaacgg
     3661 gaagacgatg aaggaagtat tagaagcata tatgcaaagg tatagtgtta aagagctatt
     3721 aacggattta aaagaagaaa caatagataa ttagaatctg aatacctcgt ttaaaccttg
     3781 aatacatgta catttttaag gcttaaattt tatagttggt aaaagtagaa aaaacacgac
     3841 ctatcaataa aaatatcgct aaatttacgc taaacaacta taagtattct tcctaagata
     3901 gtggtgaacc aggaggcatt catgtgaggg catagtcttt gcggtttacg gtatgtcggt
     3961 ttttagggaa gaaatggcga ggtttaacga ggattggcga agaattaagg gttaaattaa
     4021 ttggttttca tcgagtatta gcgaagatta gggaagacca ctaaggaatc aaagcgtagc
     4081 ggttcattta caatgagaat gtccgtggta gcctgttatg gcttgccaat ccttgtttga
     4141 gcgttagcga gttgaaggag tggggcattg gcttatgacc cacggactgg aggctcgttg
     4201 tcaatgattg aattccgctt attggaggaa gtaagttatg gatatagaag ctaagaaaga
     4261 agcatttgtg accttccttg tagagaaagt tctatttcaa acatttgcag aaaggactac
     4321 ttctttagaa gaaaaagagg tgtatgaaaa gtttattaag ctatatgatc aagaattaaa
     4381 tagactaaat ttcgaattga caatagcatc tgtaaataga aaaagagcca acctataatg
     4441 ttatagatta gctctttttg tttagaagaa gaatgtataa attcggatta gatttccaac
     4501 aaaattgacc acgtcttttg ttattccgac tattttcatt gctgtcttca tttgtttctt
     4561 aggttgtttc ttaggttgtt tttttgcttg ttttttagtt ttcttcataa attaattccc
     4621 cctatttagt agttgttcgt cttgctatct attacttgtg acgttttttg agaaatcctt
     4681 atttttttta ttctttttca aaaaaatttc ttttataaac ataggcgcag atactaaggt
     4741 tttttggttt gctatatgta taaaacttga gtaaaaatat caatttggat tgtcaatgga
     4801 ataacacctc ctagttttct attcgtcttt tcatctatgt tttgtgacac tttttgagag
     4861 atttgtatta agagtttttt tcttcaaaga gataggcaca ttatattgtt cgtgttggtg
     4921 tgattctttt cttagttaaa ttttagagct tttcttcata gtatttatcc ttctctttat
     4981 ccttaattta tccgtgattt atccgtcttt ttatcgagat tatcctctat agaaaagtgt
     5041 atcttggat
//
