LOCUS       NZ_AP022998             8229 bp    DNA     circular CON 14-MAR-2024
DEFINITION  Bacillus cereus strain 30075 plasmid p30075_4, complete sequence.
ACCESSION   NZ_AP022998
VERSION     NZ_AP022998.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203342
            Sequence Read Archive: DRR206382, DRR206406
            Assembly: GCF_021655335.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8229)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 424x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655335.1-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 03:49:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,110
            CDSs (total)                      :: 5,958
            Genes (coding)                    :: 5,598
            CDSs (with protein)               :: 5,598
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 216 of 360
            Pseudo Genes (incomplete)         :: 158 of 360
            Pseudo Genes (internal stop)      :: 51 of 360
            Pseudo Genes (multiple problems)  :: 59 of 360
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022998.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="30075"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="p30075_4"
                     /geo_loc_name="Japan:Tottori"
                     /collection_date="2016"
     gene            160..1272
                     /locus_tag="L6490_RS30300"
                     /old_locus_tag="BC30075_p41"
     CDS             160..1272
                     /locus_tag="L6490_RS30300"
                     /old_locus_tag="BC30075_p41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002019875.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_000036645.1"
                     /translation="MSKITNDIDLKIDEVYEKVKDMKITFDENNTTKKQLTDSTINTYC
                     GIVKSYNNWLIQTHGISVDRAKPRHAMEYLETKIQAFEKGNASAFSMRQMAHAFHSFRV
                     ASAETGTFKHTCKVADKRDMLNTLTERGIFRKSADSTVLKANHSDYEKVQKELNVATTS
                     KHLREDIKTVHELQRYAGMRVHEAVKLKVSDIDFKSGTVYIKGKGGHERTVKIESKELL
                     EKLKNLCTGKSERSQVIIARKRDKNQLTNEEFGKRAKEEIRGAAVNAGVDRNGKRYTTH
                     SGRKAYAQAQMDKYKSYSKNQLKKEVAKRCQDKKTKQKYDAVIKNIRKKITEGSKNEGR
                     KLTNKEMCQFLVSLDLGHYRLDVVRYYADY"
                     /name="tyrosine-type recombinase/integrase"
     gene            2085..2492
                     /locus_tag="L6490_RS30305"
                     /old_locus_tag="BC30075_p42"
     CDS             2085..2492
                     /locus_tag="L6490_RS30305"
                     /old_locus_tag="BC30075_p42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000037919.1"
                     /translation="MSKKISIEEMTQEMKEKFTISLEDAHKILLDHGVTKSTNTQVTMR
                     WIRDGKIDALLIGKGRVETRKWLINKESLMEFVAFERLTLGDFKAMKEELEMLRAFKFE
                     VEKKPKRATGTRQKKDAATNTSKEKKEVPTK"
                     /name="hypothetical protein"
     gene            2506..2943
                     /locus_tag="L6490_RS30310"
                     /old_locus_tag="BC30075_p43"
     CDS             2506..2943
                     /locus_tag="L6490_RS30310"
                     /old_locus_tag="BC30075_p43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000094076.1"
                     /translation="MSSFRWYEENEVIGKTIIEEDDLTLLEAGKIYIDAIFCERIRFPV
                     QKNEQPIGFNFVYEYNVKKYFGLYDRSFMKIIKQEDLEEYHMRMIDFHVHRLSLGEELD
                     DCPIIGICYGSGPLITPRLEQVYDVSKTKAYRKYRLEKLFD"
                     /name="hypothetical protein"
     gene            3194..3814
                     /locus_tag="L6490_RS30315"
                     /old_locus_tag="BC30075_p44"
     CDS             3194..3814
                     /locus_tag="L6490_RS30315"
                     /old_locus_tag="BC30075_p44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000963189.1"
                     /translation="MAIDFSQADRNARKRDLEKEKFFSDEEWELANDLQSRANARGMKL
                     VPERKIKNRAKFAQMLQDNVDYLEEIDYLDDKEIIFLWKISRKVGFLSNCLVDDIHKKS
                     PVPLTQTDIATLLKRTKNNVNPIVKSLVAKGILAVSKTGVQGNNSRANVYFMNPNILYA
                     GSKDDVNLTLQAMFSKVPKALKDMPVKLFELSCHSEEEEEKTN"
                     /name="hypothetical protein"
     gene            complement(4499..5937)
                     /locus_tag="L6490_RS30320"
                     /pseudo=""
     CDS             complement(4499..5937)
                     /locus_tag="L6490_RS30320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053955.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /name="IS4 family transposase"
     gene            complement(6267..6644)
                     /gene="qacH"
                     /locus_tag="L6490_RS30325"
                     /old_locus_tag="BC30075_p47"
     ARG             complement(6271..6644)
                     /name="qacH"
                     /gene="qacH"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacH"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(6644..7195)
                     /locus_tag="L6490_RS30330"
                     /old_locus_tag="BC30075_p48"
     CDS             complement(6644..7195)
                     /locus_tag="L6490_RS30330"
                     /old_locus_tag="BC30075_p48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_000028030.1"
                     /translation="MSISKHANKKKNILIAASEIVKEEGVVKLTLEAVAQRAGVSKGGL
                     LYHFPSKEALIKGMVEDWTNNYFECINTLVNNDDDNAIGKWNRAYLKSTFSDLENNNLN
                     SALMATMFINPDLLDEFRQRYDILHTKLITDGIDPVKITITRLSIDGLWFSEIFGMAPL
                     NEELKTQVFDELINMIQEDE"
                     /name="TetR/AcrR family transcriptional regulator"
     ARG             complement(6268..6644)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="60.75"
                     /coverage="116.82"
ORIGIN
        1 tgtgtgttta atttgggtgg ttcggtctat tttttttggt ctttaaaaaa aatagatcga
       61 acccacccaa atgagacctc gtttttgttg tgccttgata ttgtaaggtt cacgggggtt
      121 gggttcttgc tcttgatttt gtatataggg ggtgacgaaa tgagcaaaat tacaaatgat
      181 attgatttaa aaattgatga agtctatgaa aaagttaaag atatgaaaat aacttttgat
      241 gaaaataata caaccaaaaa acaattaact gattcaacaa taaacacata ttgcggaatc
      301 gtgaaatcat ataacaattg gttaatacaa acgcatggaa ttagcgtgga tagggcgaaa
      361 cctcgccacg caatggaata tctcgaaaca aaaattcaag cctttgaaaa aggaaatgct
      421 tccgcatttt ctatgcgcca aatggcgcat gcgtttcatt cttttcgtgt tgcgagtgct
      481 gaaacaggaa cgtttaaaca tacatgtaag gtcgctgata aacgagatat gttaaataca
      541 ctcacagagc gtggaatctt ccgtaaatcg gctgattcga cggtattaaa ggcaaatcat
      601 tcagactatg aaaaagttca aaaagaacta aatgtagcaa cgacaagtaa acatttgaga
      661 gaagatataa aaacagtaca cgaattacag cggtacgctg gaatgcgtgt tcacgaagcc
      721 gtaaaactaa aagtgagtga tatagacttt aagagtggca ctgtctatat aaaaggcaaa
      781 ggcgggcacg agagaacggt taaaattgaa agtaaagaat tactagagaa gttgaaaaat
      841 ttatgcactg gaaaaagtga aagaagccag gtaattattg ctcgaaaacg tgataaaaat
      901 caattaacaa atgaggaatt tggaaaacgt gcaaaagaag aaatacgagg tgcagccgtc
      961 aatgctgggg tggatcgtaa cggaaaaaga tacacaacac attcaggtcg caaagcatat
     1021 gcacaagcgc aaatggataa atacaaaagt tacagtaaga atcaattaaa aaaagaagtt
     1081 gcaaaacgtt gtcaggacaa gaaaacaaag caaaaatatg atgcagttat aaaaaatatc
     1141 cgtaaaaaaa taaccgaagg ttcgaagaac gaagggcgta aacttacaaa taaagagatg
     1201 tgccagtttc tagttagctt ggatttgggt cactatcgtt tagatgttgt acgatattat
     1261 gctgattatt gataggtttt gacggaaaat cgggaagacg agtgcggagc acagttggta
     1321 ctggcgcact ggcttagaaa cacaagggct agagcctcgt tgtcaagcca tctttcccgg
     1381 ctttacaatg agaaagcctt gtgttagcct ctcatggcaa tgccagtacc ttgtgcggat
     1441 tcgaggaaat cgccaaaaaa cgggtcgatg aactaagaag atacacgaga gttttccaaa
     1501 tcctcgccat ttctcgcttt ttttcgaaaa tcaaaaaaaa atgaaacgcc tattaaattt
     1561 actatacagt aaaagtattt taaaatacat ttattgtata gtaaatttat ttttgagtaa
     1621 aagtatttta aagtaatttt aatagtttta cacaatagta aatttatata cctaaattag
     1681 atttacacaa aagtaaaaat atagataaaa atacatgtat tgtctccgca atttattttt
     1741 agtaatcgca aaaaatcgtt gatttaacaa cgtttaagag atataaaata catgtacaaa
     1801 atagtaaagt catggcaata aatagattta cacaaaagta aaagtaatga cctaaaatgg
     1861 atttacataa aaataaattt aatgtataat aaatttaaag aaggatatat ataattttga
     1921 gtaaatataa ttaatagtaa atgtaaaata gattaaatat attttttaat aaatttattt
     1981 aaaattatat ttacttttca atacatttac tgtatattaa aaatatagat ggatacattt
     2041 aattaaaaat acatgtactt aattgtaatt aggaggaaaa aacaatgtca aaaaaaatct
     2101 caattgaaga aatgacgcaa gaaatgaagg aaaaattcac gatttcactg gaagatgcac
     2161 ataaaatcct tttggatcat ggcgtaacga aaagtacgaa tacacaagtt acaatgcgtt
     2221 ggattcgtga tgggaaaatt gatgcattat tgattggaaa aggtcgtgtg gagactcgaa
     2281 aatggcttat taacaaggaa agtctaatgg aattcgttgc attcgagcgt ttaacgttag
     2341 gtgatttcaa agcaatgaaa gaagaattag aaatgttacg tgcttttaaa ttcgaagtag
     2401 agaaaaagcc aaagcgtgct actggtacac gtcaaaagaa agatgcagca acaaatactt
     2461 caaaagaaaa gaaagaagtg ccaacaaaat aaagaggtga attgcatgtc atcttttaga
     2521 tggtatgaag aaaatgaagt gattggaaaa actataatag aagaagatga tttgactttg
     2581 ctagaagctg ggaaaatata tattgatgca atattttgtg agagaataag gtttccagtt
     2641 caaaaaaacg aacagccaat tggatttaat tttgtttatg aatataatgt aaaaaaatat
     2701 ttcggattat atgacagaag tttcatgaag ataattaaac aagaagattt agaagaatat
     2761 cacatgagaa tgatagattt tcatgtgcac agattatctt taggggaaga attagatgat
     2821 tgtccaatca ttggtatttg ttatggaagt ggacctttga taacaccgag gttggaacaa
     2881 gtttatgatg tttcgaaaac aaaagcatac agaaagtatc gattagaaaa attattcgat
     2941 taggtaaagc atcttacaaa atttgagttt ttaagctaga attgaataga caagcataaa
     3001 aaaaagccga gtgttggagc actcgacttt gggtgattca tcgtaattta tcaccaaaac
     3061 gttgatttct agttttaatt ttaatgaatt ttcatttaaa aatcaagggt aaaacccttt
     3121 acggataata aaaaaataac ggtgaatctc ctagaatgct tgttgcattt tcttcaggag
     3181 aggagcaaaa tcaatggcaa ttgattttag tcaagcggat cgaaacgcta gaaaaagaga
     3241 cttagaaaaa gaaaagtttt tttcagatga agaatgggaa ttagcaaatg atttgcaatc
     3301 aagagctaac gcgcgaggaa tgaaacttgt tccagagcgt aaaatcaaga atcgagcaaa
     3361 atttgctcaa atgcttcagg ataacgttga ttatctcgaa gagattgatt atttagatga
     3421 taaagagatt atatttttat ggaaaatcag tcgtaaagtt ggttttttat caaattgtct
     3481 tgtggatgat attcataaaa aaagtccagt tcctttaaca caaacagata tcgcaacttt
     3541 gttgaaaaga actaaaaata atgtcaatcc tattgttaaa tctttagtag caaaaggcat
     3601 actggctgtt tcaaaaacag gtgttcaagg taataattcg agagcaaatg tgtattttat
     3661 gaatccgaac attttgtacg ccggtagcaa agatgatgtc aatttaacat tacaagcgat
     3721 gttttcaaaa gttcctaaag cattaaagga tatgcctgta aaattattcg aactatcatg
     3781 tcattcagaa gaagaagaag agaaaacaaa ttaataataa ttaatacatg tattttttaa
     3841 tacatgtatt tttttatctt tcaattatgt aaaataatgt aaaaaatgta aaaaatccgg
     3901 tgttcttaaa ttcaagaact atggttctta aattcaagaa ctacgaaaag tgcaacaaac
     3961 cattgatatt attgactttt taatacatta acaatgttgt tctatctcac tctctattaa
     4021 cgctaatttt gagtaaagaa acgagtagta ttttttgcaa aaaccgcaaa aaatctccac
     4081 tctttatctt gactcaaaat tctccgcaat tggcatggct tgtgccaagc gataaaccaa
     4141 aaaagttatc actttttcgg attatcactt gaacaatcca ttttaattgc tccgtcgctt
     4201 agtgcgtaca tttccgttct atcaatattt tgtgtaataa aaacccttgg ggctagcccc
     4261 aaaccccagc cagctcaacc caaaaaagca tatgaaaacc gtatcaaatt taatacggta
     4321 aaagaataaa agacttaaaa acatttttgc ttaggaaagg ggtagcgtca catgcccatc
     4381 aacttaagaa cgaaaacaaa atgaactttc gttcaaacaa actaaaaaac acgtaatgta
     4441 cttttggtat tgaaaagtac acacaccaaa taaactctaa cgagtttcat tttaaaaatt
     4501 atgcagcttt cttttgtttt ccaaacccat tatactcata gacaacaccc ataatatcaa
     4561 agatagtttt cttctcatat cgatgagatt tccgtccatt cttctgtagg aggtcgaaca
     4621 aacggattaa aatctttgtt agattttggg tgtttctctg tatcgcttga tataacagcg
     4681 atacatgatc ttgaatcatc ccaatggctt tatattcact taattctctt ttgttctttt
     4741 gcaaaagaag tttacgcatc ttaaacatcg tagaagaaca gataaagatg gcaataagtc
     4801 ttccatacac atgacattct aatcgctctt ttttgatagt ttgccaatga tgaatttgaa
     4861 ataaggattt ccacgtttta aaaataattt ctatttgcca gcgaagggag taaaagtcat
     4921 gtatttgttc catcggaaca actccttcag gcgtatttga gacatatatg ttgatacccg
     4981 ttaatcgttt actcttttct gaaaatgtaa tacccttttt actttcagtg taactttgtt
     5041 tttttctacg ttcctgtatc tgtttgtctg ttaaacgata gataattaca cgtgtgaata
     5101 gtttttgatt ctttccaatg taagcttctt ttatttcgta tgtttgaccg ggttttaaat
     5161 tgttcataat atgttctaaa tcaacttgga tatactgtga ttgctttttt tacggttccg
     5221 tttcgaaagt attctggaaa cggatttttt tacatacacg gtatggttta attttaatct
     5281 ggatatataa taagcacctc gttgatccat ttgatctaag tcttctaatg aaaaataacc
     5341 taaatcacgg atacataaat cgcttggacg taatgtgtct aagcattctg ttccaaaggt
     5401 cttatcatta ttttttcctg gacctatctg aaagtttaga aactgtccgc tatgtaagtc
     5461 atattctaat tgaatcttga tacccgctgt ttgtgcacaa ccacctgatc caggatatac
     5521 atgggctaaa tgttttggta cttggaaaat cgtcgcatct aaaatacgaa ttcgttgaaa
     5581 atacgtgagc gccgcgcttg aaatggccga tgtttcacag attttacttt tccataatgc
     5641 cgaaaaaata tatttcaaaa attcaaccgc ttttttatca aagcgtttat tgagtccttc
     5701 tggactcata agagtgcctg tggccgcatg taattgactg catagtcgaa caagcgagtc
     5761 actcgctgta cgttgactta cccaaataca tatggtagct aattcatttc cagaaaactt
     5821 tcgttttctt tttacaaaac ctaattcttt tgcgagttct tccaaaagag aaggggttaa
     5881 atgacgatac agttcttcag aaaatagttg taattcatct tgaatcgaaa gattcataaa
     5941 aaatgtcacc ctttctagct agctttttag aaagaataac gttttttgtc acttgggggt
     6001 agtgaaaatt cttaagttga tgggcatggg gtagcgtcag ggaaatgcgg atttacaacg
     6061 gtaagcattt gatacaagtc ttatgagaca gtcagaagtc atttaaaacg ctaatttcat
     6121 tatgtctcaa taaactctac tctattggaa ttgtgaagtg atacacttaa actaacgtac
     6181 gcaggtttgt tgaataagga cttcaaaaaa tacccttaat taagctaaaa agtccttctc
     6241 tttattaaga aggacttttt tatgtgttat tccattgtct cattcattgt aatagtttcg
     6301 cttgcttcat gaccagattc tccatgtcct ggtccaaaca aattcaatat tacaactcct
     6361 acaagaatca gtgttattcc tgcaatgcta gccatgttaa gtttttcctt ccaaactaaa
     6421 actgaaatta ctgtagtcaa aacaagtcct aaacctgccc ataatgcata tgcagtgttt
     6481 aaggggattg tttttagcga caaggataaa aagaaaaaac aaccaacgaa agctattatt
     6541 acccctattg taggatagag ttttgaaaag ccttcagatg cttttaatag ggaagagcca
     6601 ataatctccc ccactattgc aagtgctaaa tatagatatg acattattcg tcctcctgaa
     6661 tcatgtttat tagttcatca aaaacttgtg tttttagttc ttcatttaat ggtgccattc
     6721 caaatatttc tgaaaaccaa agaccatcta tggataatct tgtaattgtt attttgacag
     6781 gatcaattcc atcagttatt aactttgtat gcaaaatatc gtatctctgc cggaattcat
     6841 ctaataaatc tggattaata aacatagtag ccattagtgc ggaatttaga ttattgtttt
     6901 ctaaatcgga aaatgtagat ttaagataag ccctattcca ttttcctatt gcgttatcat
     6961 cgtcgttatt aactaacgta ttaatgcatt cgaaataatt atttgtccaa tcttccacca
     7021 ttcctttaat taatgcttcc ttgctaggaa aatggtataa taatccacct ttactcacgc
     7081 cagctctttg ggcgactgcc tctaatgtga gtttgacaac tccttcttct tttacaattt
     7141 cagatgcagc aatcaatata ttttttttct tattagcatg ctttgaaata gacataataa
     7201 cctccataac ttagatacat ataatatacc gtctggacgg tttttttgtc aatcgaatta
     7261 aagctcttat ttaatatcac tctttcacat aaaaataatc caataaatta atctaaagat
     7321 tgtgaagaaa tgtacttaaa gtaaagggag ctttagttca acagtcgatt tcttaaaatc
     7381 ggctttattt atgggtgaaa attacattac cgttgtaaat ccgcattttc aggactctac
     7441 cccggaaaga aaaacattta aggtcgtaag accttttcaa aagcataaaa aaataaggtt
     7501 ttttcatcaa aatcagttga ttaatggtaa aatttgtata tattcatagt aattttaaaa
     7561 ccaattactt atttgtttaa acgcttgcgg ttaagcaaat atgaaatacg aaattttata
     7621 ctatgaatct tgggacgtga ttcaagtaat cgtgtatttt acaaaactaa aaaattcacg
     7681 tcataaaaat gaaaggggat cattacaata gcaaagcaaa atgtttttaa gacgtgggaa
     7741 aaaataatct aagaaaggag caatataaag ctttcccaag tagttttata aatagcttta
     7801 aaagaaaata gaattcttgc ttgcaagaat ggaaaaaaat attataaaaa ataaaaattt
     7861 aaggagatga caaatcaaaa aatacgaagg gaacaagttt tacaattaaa tatttttaca
     7921 actgaataaa tgtaaatctg taacaaaggt ttttaaccac aaaacaaatc gcaagaacag
     7981 taatttctgg gttactcaaa aattccagac cgcaataccg ttgtacgctc atttgactat
     8041 acattttggt gcaaaatgta tgggagagcc ttggagtcta tttatagacg ccaatcgcaa
     8101 caagatggcg tagctatctt gagcctttgt cttttaatct tttgaccttg atttttgctt
     8161 ttggtctttt gattttgact ttgactctag tgagcttaaa tcgcccgaag ggagatttcg
     8221 cgattgctt
//
