LOCUS       NZ_AP022997             8319 bp    DNA     circular CON 14-MAR-2024
DEFINITION  Bacillus cereus strain 30075 plasmid p30075_3, complete sequence.
ACCESSION   NZ_AP022997
VERSION     NZ_AP022997.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203342
            Sequence Read Archive: DRR206382, DRR206406
            Assembly: GCF_021655335.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8319)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 424x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655335.1-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 03:49:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,110
            CDSs (total)                      :: 5,958
            Genes (coding)                    :: 5,598
            CDSs (with protein)               :: 5,598
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 216 of 360
            Pseudo Genes (incomplete)         :: 158 of 360
            Pseudo Genes (internal stop)      :: 51 of 360
            Pseudo Genes (multiple problems)  :: 59 of 360
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022997.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8319
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="30075"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="p30075_3"
                     /geo_loc_name="Japan:Tottori"
                     /collection_date="2016"
     gene            join(8218..8319,1..48)
                     /locus_tag="L6490_RS30230"
     CDS             join(8218..8319,1..48)
                     /locus_tag="L6490_RS30230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788727.1"
                     /translation="MEREKCSLKEAKNAYNRKWRAANRDKVKLYNDRYWEKKAKEMEEQ
                     ACQK"
                     /name="hypothetical protein"
     gene            36..308
                     /locus_tag="L6490_RS30235"
                     /old_locus_tag="BC30075_p301"
     CDS             36..308
                     /locus_tag="L6490_RS30235"
                     /old_locus_tag="BC30075_p301"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788729.1"
                     /translation="MSEVSMETVIKGKQQSELLKHLEKVGIELLGRRDELLEQWEKEGL
                     KEDSIFEDDLKFVEELMNRNDELMFDVKVELITTMDEIHHQKMGY"
                     /name="hypothetical protein"
     gene            313..564
                     /locus_tag="L6490_RS30240"
                     /old_locus_tag="BC30075_p302"
     CDS             313..564
                     /locus_tag="L6490_RS30240"
                     /old_locus_tag="BC30075_p302"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788731.1"
                     /translation="MLGKYDGLLEKERLLKRIDELSFALEASGDPMLEAMVKNSGNTDI
                     AVAKELITKGSELGMNEHVFKKALLAKVNELREEVTKL"
                     /name="hypothetical protein"
     gene            561..974
                     /locus_tag="L6490_RS30245"
                     /old_locus_tag="BC30075_p303"
     CDS             561..974
                     /locus_tag="L6490_RS30245"
                     /old_locus_tag="BC30075_p303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788733.1"
                     /translation="MKKDKQKDLPFNHKVVLSVLPLGRSKAITTKEIMARSGIKDKRAV
                     SAILSELVTVYKQPIATTSKKEFKGVFIVQDVIDYQIGKEGLVSRKRHIDKRDSAYTEA
                     CLEIFKGTVTLDESKNKVEGSVRACKQSASSSM"
                     /name="hypothetical protein"
     gene            944..1651
                     /locus_tag="L6490_RS30250"
                     /old_locus_tag="BC30075_p304"
     CDS             944..1651
                     /locus_tag="L6490_RS30250"
                     /old_locus_tag="BC30075_p304"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788735.1"
                     /translation="MQTISKFFNVDNESKLNELTIAKGELNSKESELQSKLSRVERALE
                     LAQTDLMIDDSTANKKVVAKYEEAQEKFQSELIATQEELNKVSDEIAAIVKEELHAELV
                     DAAKQDVMLIELPEKCRKAKKVINRDLYRATSFEDEGRFSRKYLHVSATVIDDARTFAD
                     IHRFADKPNLTRIGYQRGELIRWGGDNNLFKLNDEYETIYEEEAQKIKDKTDKEYEQFM
                     KAFNKYFAVSDSH"
                     /name="hypothetical protein"
     gene            1705..2130
                     /locus_tag="L6490_RS30255"
                     /old_locus_tag="BC30075_p305"
     CDS             1705..2130
                     /locus_tag="L6490_RS30255"
                     /old_locus_tag="BC30075_p305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788712.1"
                     /translation="MSKDTLKNIFHVYCFYTVRLQGDTEPRMRRNKLNYDNPIQNYYES
                     FIDCLRGFCEVRSDVSLYSFYQFVVDAVNSLNKQERKLIYERYLYKDHYKSDRQHYLAM
                     DITPQNYKKQMDPVRRKLIKNLGIEDLQLNIPDWMKR"
                     /name="hypothetical protein"
     gene            2261..2509
                     /locus_tag="L6490_RS30260"
                     /old_locus_tag="BC30075_p306"
     CDS             2261..2509
                     /locus_tag="L6490_RS30260"
                     /old_locus_tag="BC30075_p306"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_236788716.1"
                     /translation="MKSLYRILELDKDEVSSSEKLILLTIAIYSDNKFIPFSITELENY
                     TNLSRATVTRLVKQLEEKGFVEVQRNGTATNNYKVTL"
                     /name="helix-turn-helix domain-containing protein"
     gene            2586..2762
                     /locus_tag="L6490_RS30265"
                     /old_locus_tag="BC30075_p307"
     CDS             2586..2762
                     /locus_tag="L6490_RS30265"
                     /old_locus_tag="BC30075_p307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176543923.1"
                     /translation="MLPQKEQLGHLAEKTELVKDLAWIAHDLLTEEDYTKENAAEALIK
                     VINRELSYVSKVR"
                     /name="hypothetical protein"
     gene            2804..3010
                     /locus_tag="L6490_RS30270"
                     /old_locus_tag="BC30075_p308"
     CDS             2804..3010
                     /locus_tag="L6490_RS30270"
                     /old_locus_tag="BC30075_p308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788718.1"
                     /translation="MANTVAKDKFEIVEKFVNACMLKGVSEDKSFKLVEELCTLIQQKR
                     HDEWFGLVDEALKEAGHEEGVTL"
                     /name="hypothetical protein"
     gene            3007..3138
                     /locus_tag="L6490_RS30565"
                     /old_locus_tag="BC30075_p309"
     CDS             3007..3138
                     /locus_tag="L6490_RS30565"
                     /old_locus_tag="BC30075_p309"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272880692.1"
                     /translation="MSEVEMVLHEFEELLHKIISAAQHGDEQAMNHLDIVKAWLKDN"
                     /name="hypothetical protein"
     gene            3215..3454
                     /locus_tag="L6490_RS30275"
                     /old_locus_tag="BC30075_p310"
     CDS             3215..3454
                     /locus_tag="L6490_RS30275"
                     /old_locus_tag="BC30075_p310"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788720.1"
                     /translation="MAEEHRYHVTFYFSNGKEFDGKVTSKYNKEDYLEGLEDLFLKEKT
                     LLIDHLGMLIQTKYITHVKVIEVGSKIGADKKDT"
                     /name="hypothetical protein"
     gene            3515..4051
                     /locus_tag="L6490_RS30280"
                     /old_locus_tag="BC30075_p311"
     CDS             3515..4051
                     /locus_tag="L6490_RS30280"
                     /old_locus_tag="BC30075_p311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3967 domain-containing protein"
                     /protein_id="WP_236788722.1"
                     /translation="MTDKTDSSQSIYISKDVAMMLKIQESTLRKYCIMLEEHGYKFHKN
                     EHGHRGFLDNDVITLRKLIEIKSHPDMTLKQACNAIMTWVKEKDMSEVDTNVITENEQH
                     DERYSELKEMIQQQNELLKKMAKKLDDQQRYIDEKLEKRDQQLMGAIREMQEEKRVLLE
                     TASASQEKKWYKFWK"
                     /name="DUF3967 domain-containing protein"
     gene            4675..5274
                     /locus_tag="L6490_RS30285"
                     /old_locus_tag="BC30075_p312"
     CDS             4675..5274
                     /locus_tag="L6490_RS30285"
                     /old_locus_tag="BC30075_p312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562260.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_236788723.1"
                     /translation="MNKRFAYIRVSDKTQNENRQLDEMLQQEIEERDIFIDKASGKDFN
                     RPKYQALKQMLRAGDILYIKSIDRFGRNKDDILKEWNEITKEIGADIVVLDMPLLDTRQ
                     YKDVLGSFVSDLVLQILSFFAEEERRKIKERQREGIDAAQRRGAKFGRPAATFPPGFEE
                     VYKRWKDGEIKAIEAMEELELKRTTFYKLVNSFENK"
                     /name="recombinase family protein"
     gene            6030..6227
                     /locus_tag="L6490_RS30290"
                     /old_locus_tag="BC30075_p313"
     CDS             6030..6227
                     /locus_tag="L6490_RS30290"
                     /old_locus_tag="BC30075_p313"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788725.1"
                     /translation="MIGKDMITLDNPKKEYFQQYAQNNKEQINANARRRRQQQKEKVEK
                     YDRLVAALRSRGISVEELLK"
                     /name="hypothetical protein"
     gene            6650..7750
                     /locus_tag="L6490_RS30295"
                     /old_locus_tag="BC30075_p314"
     CDS             6650..7750
                     /locus_tag="L6490_RS30295"
                     /old_locus_tag="BC30075_p314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205124.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_098492605.1"
                     /translation="MNHEEFINSTEIDDKFLKQIIDNREFNDVIVSYYERLHKESRDDG
                     MKKKQENVKGCNDFWIIDKYHEHRLKEFKRTNLCKDKFCNNCKKVKQASRMGKFIPLIQ
                     PYAENMYQLTLTIPNVKGDELKDTLSKMQKAFARLIEYFKGKKKINGIDFEVLQYEGAI
                     RSLEITFNGNEYHPHYHALIVLKVNKFHDCMIRKHKNVYSIDFTGKREARLFSDIEVLI
                     QKVWYLIINGKRITETAIQSLKKGYSCQLDKFSENDFVEIFKYMTKATTEDGKTLSYKQ
                     FKTLYYALFKVRQIQGYGIFYNLKDDEEINFDEVNEAYAEFIEELQTKEKPVTVSEAPE
                     QVRKESDYTVFSRKTFQKYWKQVMSE"
                     /name="protein rep"
ORIGIN
        1 tgggaaaaga aagctaaaga aatggaggaa caagcatgtc agaagtaagt atggaaactg
       61 ttattaaagg aaagcagcag agtgaactgt tgaagcattt agaaaaagta ggtattgaat
      121 tactggggcg tagagatgaa ttgttagagc agtgggaaaa agaaggactt aaagaagaca
      181 gcatttttga agatgatctt aaattcgttg aagaattaat gaaccgaaat gacgaattaa
      241 tgtttgatgt aaaagtggaa ttaattacaa ctatggatga gattcatcat cagaaaatgg
      301 ggtattgata ggatgttagg aaagtatgat ggattattag aaaaagaacg gttacttaaa
      361 cgaattgatg aactgtcatt cgcgttagaa gcttcaggtg accctatgtt ggaagcgatg
      421 gttaaaaata gtggcaatac agacatcgcg gttgcaaaag agttgattac aaaagggagt
      481 gaattaggta tgaatgagca cgtatttaaa aaagcgttgc tagcaaaagt aaatgaactt
      541 cgtgaagagg taacgaaact atgaaaaaag acaaacaaaa agacctccct tttaatcaca
      601 aagtagtatt gagcgtacta cctttaggaa gatctaaagc aataacaact aaagaaatta
      661 tggcacgtag tggaataaaa gacaagcgtg cagtatctgc tattttaagt gaattagtga
      721 ctgtatataa acaacctatt gctacaacgt ctaaaaaaga atttaagggc gtttttattg
      781 tacaggatgt aatagattat cagattggta aagaaggcct tgtgagcaga aaaaggcata
      841 tagataagcg tgattcagct tatacagaag cgtgcttgga aatattcaaa ggtacagtga
      901 cattagatga gtcaaaaaac aaagtggaag gaagtgttcg tgcatgcaaa caatcagcaa
      961 gttcttcaat gtagataacg agagtaagtt aaacgaatta acaatagcaa aaggggaatt
     1021 gaattccaaa gaatcagaat tacaaagtaa gctatctcga gtagaaagag ctctggaact
     1081 ggcacaaact gacttaatga ttgatgatag tacagctaat aaaaaggttg ttgcaaaata
     1141 tgaagaagct caagagaagt tccaatcaga gttaatagct actcaagaag aacttaacaa
     1201 ggtttctgat gagattgcag ctatcgttaa ggaagaattg catgctgaat tagtagatgc
     1261 cgctaaacag gacgttatgc taatcgaact acctgaaaag tgcagaaaag cgaaaaaagt
     1321 aattaacagg gatctttata gggcgacgtc ctttgaagat gaaggtcgat ttagtagaaa
     1381 gtatttgcat gttagtgcta cagtgatcga cgacgctcgt actttcgccg atatacatcg
     1441 ttttgctgac aaaccaaatc taacacgaat agggtatcaa cgcggtgaat tgatacgttg
     1501 gggtggcgat aataacctgt tcaaattgaa tgatgaatat gaaacgatat atgaagaaga
     1561 agcacagaaa atcaaagaca aaacggataa agagtatgag cagttcatga aggctttcaa
     1621 taagtatttc gctgtgtcgg attctcatta atcaaaatac ataagtcgcc taagggcggc
     1681 ttttttttat taggagggat tttcatgagt aaagatacat taaaaaacat ctttcacgtg
     1741 tattgttttt atacagtacg tttgcaaggc gatacagaac cacgtatgag acgaaacaag
     1801 ctaaattatg ataatcctat acaaaactat tatgaaagtt ttatagactg tctgagagga
     1861 ttctgtgagg ttaggagcga cgtttcattg tattcgttct atcaatttgt tgtagatgct
     1921 gttaattcgt taaataagca ggaaagaaag ctgatctatg agcggtattt atataaagat
     1981 cactacaaat cagataggca acattacctt gcgatggata taacaccaca gaattataaa
     2041 aaacagatgg atccggtaag aaggaagtta attaagaacc tcggaataga agatttacag
     2101 ttgaatattc cagattggat gaaacgataa cgggttaaat tacatgtatg gttatattat
     2161 ttttaaattt tattttgatt ctattgcatt ttactatact cattgttata ctagataatg
     2221 taaacaacat gttgatttat aggaggttac tagtttaatt atgaaatcac tatatcgtat
     2281 tcttgaattg gacaaagatg aagtttcttc ttctgaaaaa ctaatcttgc taacgatagc
     2341 gatctattca gacaacaagt ttatcccatt ctctattaca gaattggaga attacacgaa
     2401 tttgagtaga gctacggtta caaggctcgt aaaacagtta gaagaaaagg gattcgtcga
     2461 ggttcaaagg aatggaacag caacaaacaa ttacaaagta acactgtaaa aacaaagaaa
     2521 gagcaggaga ttgcggctcc tactctttca aaacacatta tcccttatgg aaaggatgta
     2581 ttagtatgtt accacaaaaa gaacagttag gacatcttgc tgagaaaact gaattagtta
     2641 aggatttagc ttggattgca catgatttac ttaccgagga agattacacc aaagagaatg
     2701 cagcggaagc gttaattaag gtcattaata gggaattatc atacgtttcc aaagtgcgtt
     2761 aaacgggatt atatgtaaat aaaacaattc ggaggtaata ttcatggcaa atacagttgc
     2821 taaagataaa tttgagatcg tagaaaagtt tgtgaatgct tgtatgttaa aaggtgtttc
     2881 ggaagataaa tcattcaaat tagtagagga attatgtaca cttattcaac aaaaacgtca
     2941 tgatgaatgg tttggattag tagatgaagc acttaaagaa gcgggacatg aagaaggtgt
     3001 aacgttatga gtgaagtaga aatggtttta cacgaattcg aagaactatt acataaaatc
     3061 atatcagcag cgcaacatgg cgatgaacaa gcgatgaatc atttagatat agtaaaagca
     3121 tggttaaagg acaattaagg cgtggtgatc cacgtctttt ttttatactg tcttggggaa
     3181 tcctactgat atattttgaa agtaggtgga ctaaatggct gaagaacata gataccatgt
     3241 aactttttat ttttctaacg gaaaagaatt tgatggtaag gtaacaagca aatacaacaa
     3301 agaagattat ttagaagggt tagaagatct attccttaaa gaaaagacat tactgattga
     3361 tcatctagga atgttaatac aaaccaaata tattacacat gtcaaagtta tcgaagttgg
     3421 atctaaaata ggagcagaca aaaaagatac gtgaattatt aaggaaaagg atatatcatt
     3481 gttataacga atgttttggt agaggtgaag cgttatgaca gataaaacag acagctctca
     3541 atccatatat ataagcaaag acgtagcgat gatgcttaaa attcaagaat cgactttacg
     3601 taaatattgt atcatgcttg aggaacacgg ctacaaattt cataagaatg agcacggcca
     3661 tagaggattc cttgataacg atgttataac gttaagaaaa ttaatcgaga ttaaatcaca
     3721 tcctgatatg acattaaaac aggcctgtaa cgcaattatg acgtgggtta aagagaaaga
     3781 tatgtcagag gttgatacga atgttataac ggaaaatgag caacatgacg agcgatatag
     3841 tgagcttaaa gaaatgattc agcagcagaa tgaattatta aagaaaatgg cgaagaaatt
     3901 agatgatcag cagaggtata ttgatgaaaa gttagaaaaa cgagatcagc agttaatggg
     3961 agctatccga gaaatgcaag aagaaaaacg agttttgtta gaaacggctt ccgctagtca
     4021 agaaaagaaa tggtacaagt tttggaagta ggattcccta actatgggga gtcctttttt
     4081 attttaaagt gagtttggat cacttagagg tagattaaac agaaagccct gcaggtaaca
     4141 tcctcagaaa cgcacaagaa aagtggtgcg tataactggg cactcgcttt tttctcgtga
     4201 ataagatctc attgaatgga gtaattttgt atactactcc gttggaatat agaacgtttt
     4261 acggagcgtt ccaatatcgt tacctttgca aaacaaacac gaataccacg ttactttaat
     4321 ccgcacacaa ggcacccttt ttatgcagta ctcattgtag tattgcaccc cttttaatgt
     4381 tgtactgcaa aacaaacaca actcaactaa taaagaacaa agcacatata ccggtaattg
     4441 aatggtaacg ggtgttaagg aatgataagg agtattagtg caaaatgaat gtagtagtat
     4501 tttagtaatt tgcaaaacaa acgaactcaa ttaatgtatt cgtaaaagta cacatttacg
     4561 aataagaaaa agaataagaa aaagatattg ttttgtgttc gtataagttg tatgattagt
     4621 ttacaaacat tcgtaaattg aaaaacactt ttacgaacaa aggggaagag gaaaatgaac
     4681 aaacgtttcg cttatatccg tgtatcagat aaaactcaaa acgaaaatag acagttggac
     4741 gagatgttgc agcaggagat tgaagaaaga gatatcttca ttgacaaggc aagcggtaaa
     4801 gattttaatc gtcctaaata ccaggcttta aaacagatgc tacgtgccgg agatatactg
     4861 tatatcaaat ctattgatcg ttttggtaga aataaagatg atattttaaa agagtggaat
     4921 gaaataacga aggagatcgg tgcggatatc gttgtattgg acatgccttt attagataca
     4981 agacaatata aagatgtatt aggctcgttt gtaagtgatt tggtactaca gatactatca
     5041 ttctttgcag aagaagaaag acggaaaatc aaagagagac aacgtgaggg cattgatgcc
     5101 gcacaaagac gaggtgcgaa gttcggaaga ccggctgcta cattcccgcc aggattcgaa
     5161 gaagtgtaca agcgttggaa agatggagaa attaaagcaa tagaagctat ggaagaatta
     5221 gagttaaaac ggactacttt ctataaacta gtaaattcat tcgaaaataa gtaaaaaaag
     5281 taataataat attaaacttt ttacaaaatt aagtcgaaaa agcaaagtta aaaaaagaca
     5341 aacataccga taacaagcat tgatattaca cataatttta tgcctttcat gtcgaaaagt
     5401 aaaagttaaa aaagtaataa aaaagcaaag tataaaaagt atacttttaa gtttgcttgt
     5461 ttctatgaaa atccgttggt ataatagttt taacgtgttt gagtgtttca aacgaaaaga
     5521 aaaaagccga gttggggaac tcgacttaag tctttctaaa agtatgggga tacttttaga
     5581 ttagattttg tgtctcgaca acactttcga ctaattagca ctaccaatgc ttattagtca
     5641 ttgatgattt tacgaatcat cttaacgatt atttttttat ggattgacac ctattttatc
     5701 gacctttgac aatcaaataa ttaggtacca tttatatcta agattgaatt aacaacctta
     5761 tttaacgtat gaaaaagtac tgtaggtcaa gttgtaaact ataaaaagtt tcttcttagg
     5821 aaaccttaag agaaaaaaat atagatacaa tttgtatcgc tttaatacga aaaaaagttc
     5881 tcgttcagcg aacttcctat gtgacaagtt tacaatgtat ttttcgtttt gacaatactt
     5941 taagtaaact catagttttt acatacattg aaaggtacct ctactatttg aggtgccttt
     6001 tttatttatt tttgaaagga tgatagaaaa tgatagggaa agatatgatc acattagaca
     6061 acccaaagaa agagtatttt cagcaatatg cacaaaataa taaagaacaa attaacgcca
     6121 atgcaagaag acgaagacaa caacaaaaag aaaaggtaga gaagtatgat agattggttg
     6181 cggcgttacg atctagagga attagtgtag aagaattatt gaaataagat ggacaagctg
     6241 tattaaggcc ggactaagac accaggagaa gatttgtata cggtctaggt tattcgtggg
     6301 gcttatgcta cagctgtcat tgtctagcgg tatcgcagtg cgtaagcacg agaataagga
     6361 gcagacatac accacaagcc ccgaacactt tcatgctctc caaaacaaca tgttttgggg
     6421 atacctagtg taacgtggcc gagtgcaacg gaaggtttta atgtttcgtt taaaaaatga
     6481 gtttcttcta ttacttgata tattagaaat aagtcggcta tttctctagt tttctagtaa
     6541 tgacaaattg tcgataacca tagtcatatc aagggtttaa gctattcgac ttattgctaa
     6601 taaggcttaa aaagccttcc aaaaattcca ctagaaatga ggtgagaaat tgaatcatga
     6661 ggaatttata aattcaactg aaatagatga taaattttta aagcaaatca ttgataatag
     6721 ggaatttaat gacgttatag tctcgtatta tgaacgacta cataaagaat cacgcgatga
     6781 tggaatgaag aagaagcagg aaaacgttaa aggttgcaat gatttttgga ttatagacaa
     6841 atatcatgaa catagattaa aagagtttaa acgaactaat ctttgcaagg acaagttttg
     6901 taacaactgt aaaaaggtta aacaagcttc acgcatgggt aaattcatac cacttattca
     6961 gccttacgcg gaaaatatgt atcaattaac gctaactatt cctaatgtta aaggcgatga
     7021 attgaaagat acactaagca agatgcaaaa agcttttgct cgtttaattg agtatttcaa
     7081 aggcaaaaag aaaataaacg gaattgattt tgaagtttta caatacgaag gcgctatacg
     7141 atctttagaa ataacattta acggtaatga gtatcatcca cattatcatg ctcttattgt
     7201 tttaaaggtt aataaattcc atgattgcat gataagaaaa cataaaaatg tttattcaat
     7261 agacttcact ggaaaacgtg aggcgcgttt atttagtgat atagaggttt tgatacagaa
     7321 ggtttggtac ttgattataa acggtaaacg gataacagaa acagcgatac agtcacttaa
     7381 aaaaggttat tcatgtcagt tggacaagtt tagcgaaaac gattttgtag agatatttaa
     7441 gtacatgaca aaagcaacaa cggaagacgg taaaacattg agttataagc aattcaaaac
     7501 gttgtattat gcgttattta aagttagaca aatacaaggg tatggcatct tttataattt
     7561 gaaggatgat gaagaaatta attttgatga agttaatgaa gcgtatgcgg aattcataga
     7621 ggaattacag acaaaagaaa aacctgttac ggtatcagaa gcaccagaac aggtcagaaa
     7681 agaaagtgac tatacagtgt ttagtcgaaa aacgttccaa aaatattgga agcaagtcat
     7741 gagcgaatag ccttactcat tccggcaagt ttgataaaag gctatcttta ccaccattaa
     7801 tcaaataacg gagggttttc taatatcatt atacataatt ttaattcgta tgatattagg
     7861 aaattccttc tatatcgtta attagtagag actatgaatt atttcgtagt ctttttttat
     7921 tggaaaggaa tgattgttgt tatatttaga ggtttataac aggaaaaagg cgcctgatct
     7981 cgtttgaatc agaacgaaat caagcgcctt tgggcgataa ttaaccaaga acaagttaat
     8041 taaataaatc taaaagtttt tggaagtact tatattatat cgtatatgga gttcccggac
     8101 agtaatttta gcctaaattt gcgtgaagca aatcattaat cataccacgt aaactatata
     8161 ttcgtaaata ggtaaatgtt aaaataagag taagaagaat gaaaagaagg tggaaaagtg
     8221 gaaagagaaa agtgttcttt gaaagaagct aaaaacgctt ataaccgaaa atggagagca
     8281 gcgaatcgcg ataaagtgaa gctgtataac gatagatac
//
