LOCUS       NZ_AP022993             4697 bp    DNA     circular CON 14-MAR-2024
DEFINITION  Bacillus cereus strain 30052 plasmid p30052_7, complete sequence.
ACCESSION   NZ_AP022993
VERSION     NZ_AP022993.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203341
            Sequence Read Archive: DRR206381, DRR206405
            Assembly: GCF_021655315.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 516x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655315.1-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 04:23:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,137
            CDSs (total)                      :: 5,984
            Genes (coding)                    :: 5,659
            CDSs (with protein)               :: 5,659
            Genes (RNA)                       :: 153
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 211 of 325
            Pseudo Genes (incomplete)         :: 130 of 325
            Pseudo Genes (internal stop)      :: 52 of 325
            Pseudo Genes (multiple problems)  :: 52 of 325
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022993.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="30052"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="p30052_7"
                     /geo_loc_name="Japan:Tottori"
                     /collection_date="2016"
     gene            complement(join(4611..4697,1..291))
                     /gene="qacH"
                     /locus_tag="L6499_RS30515"
     CDS             complement(join(4611..4697,1..291))
                     /gene="qacH"
                     /locus_tag="L6499_RS30515"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacH"
                     /protein_id="WP_047914402.1"
                     /translation="MSYLYLALAIVGEIIGSSLLKASEGFSKLYPTIGVIIAFVGCFFF
                     LSLSLKTIPLNTAYALWAGLGLVLTTVISVLVWKEKLNMASIAGITLIVVGVVILNLFG
                     PGHGESGHEASETITMNETME"
                     /name="qacH"
     gene            complement(291..854)
                     /locus_tag="L6499_RS30520"
                     /old_locus_tag="BC30052_p71"
     CDS             complement(291..854)
                     /locus_tag="L6499_RS30520"
                     /old_locus_tag="BC30052_p71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002078386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_088027078.1"
                     /translation="MEVIMSISKHANKKKNILIAASEIVKEEGVVKLTLEAVAQRAGVS
                     KGGLLYHFPSKEALIKGMVEEWTNNYFECINTLVNNDDDNAIGKWNRAYLKSTFSDLEN
                     NNLNSALMAAMFINPDLLDEFRQRYDILHTKLITDGIDPVKITITRLSIDGLWFSEIFG
                     MAPLNEELKTQVFDELINMIQEDE"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            1882..2439
                     /locus_tag="L6499_RS30525"
                     /old_locus_tag="BC30052_p73"
     CDS             1882..2439
                     /locus_tag="L6499_RS30525"
                     /old_locus_tag="BC30052_p73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016125306.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_048558832.1"
                     /translation="MPAEVRKLDEQRKINVELTPEQLQMLQRLIDLEQQENKDKEQFEE
                     KKKNHNFIQLYRDNMPELRWLMANHNFASSLLFFILEHMDNRNALACSYAVFEDYFGKS
                     RSTVYRAVKLLEENGFLNVLKMGTSNVYVVNEDLAWTDGNDKKKFAKYDGKILVSKKEN
                     KDFEYKSQFDKFKALRERENLK"
                     /name="replication/maintenance protein RepL"
     gene            complement(2453..2650)
                     /locus_tag="L6499_RS30530"
                     /old_locus_tag="BC30052_p74"
     CDS             complement(2453..2650)
                     /locus_tag="L6499_RS30530"
                     /old_locus_tag="BC30052_p74"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048558833.1"
                     /translation="MKVKQLVEKLEKLDQEGELMLFMRDRKRPHLGSGTLIPIKDIGVF
                     TSVDQDSNEGKYALEIKKGE"
                     /name="hypothetical protein"
     gene            complement(2650..3996)
                     /gene="mobV"
                     /locus_tag="L6499_RS30535"
                     /old_locus_tag="BC30052_p75"
     CDS             complement(2650..3996)
                     /gene="mobV"
                     /locus_tag="L6499_RS30535"
                     /old_locus_tag="BC30052_p75"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_053082646.1"
                     /translation="MSYSIIRMQKMKSNGIKGIQFHNQRERESETNPDINKADSHLNYD
                     LINGQRQIDYNEKISQVIEDNVTSGKKIRKDAVKLAEFLITSDKEFFDKISPKEQKRYF
                     ETAHEFLADRYGEKNLIYATVHYDEKTPHMHVGFVPVTEDGRLSAKDFFGQKKQLVSLQ
                     DDFNQYLKQNDFNLERGVSSSRKHVETAKYKAQTFQSMEKEAQEKYERTMGHIQEIDDK
                     SKSIANIEAKKVLGLVGMKEQDYKSLVDYATNGVAYQLQAENLQKELEKTQKEVTQLKA
                     DMQIGQDKIRYYYKDIEENLDSLAEKKALQKVKQTDIVKNYGDLIEKYNGLVNKYNDQL
                     KEKKELKQQVDTLKIENRSYQNENRELKLENGKLKGKLVQISKEFSVFKERVGKVLHAQ
                     LDRVKTFLRMNDVDRSTIKFLDDRQDKLVQDSLEKMEKPQRQKGMEMER"
                     /name="mobV"
     ARG             complement(2..291)
                     /name="qacJ"
                     /gene="qacJ"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="60.24"
                     /coverage="90.65"
     Typing          1883..2439
                     /name="rep_cluster_1925"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.818"
                     /coverage="100.0"
                     /evalue="1.87e-127"
ORIGIN
        1 aactcctaca acaatcagtg ttattcctgc aatgctagcc atgttaagtt tttccttcca
       61 aactaaaact gaaattactg tagtcaaaac aagtcctaaa cctgcccata atgcatatgc
      121 agtgtttaag gggattgttt ttagcgacaa ggataaaaag aaaaaacaac caacgaaagc
      181 tattattacc cctattgtag gatagagttt tgaaaagcct tcagatgctt ttaataggga
      241 agagccaata atctccccca ctattgcaag tgctaaatat agatatgaca ttattcgtcc
      301 tcctgaatca tgtttattag ttcatcaaaa acttgtgttt ttagttcttc atttaatggt
      361 gccattccaa atatttctga aaaccaaaga ccatctatgg ataatcttgt aattgttatt
      421 ttgacaggat caattccatc agttattaac tttgtatgca aaatatcgta tctctgccgg
      481 aattcatcta ataaatctgg attaataaac atagcagcca ttagtgcgga atttagatta
      541 ttgttttcta aatcggaaaa tgtagattta agataagccc tattccattt tcctattgcg
      601 ttatcatcgt cgttattaac taacgtatta atgcattcga aataattatt tgtccattct
      661 tccaccattc ctttaattaa tgcttccttg ctaggaaaat ggtataataa tccaccttta
      721 ctcacgccag ctctttgggc gactgcctct aatgtgagtt tgacaactcc ttcttctttt
      781 acaatttcag atgcagcaat caatatattt tttttcttat tagcatgctt tgaaatagac
      841 ataataacct ccataactta gatacatata atataccttc tggacggttt ttttgtcaat
      901 cgaattaaag ctcttattta atatcactct ttcacataaa aataatccaa taaattaatc
      961 taaagattgt gaagaaatgt acttaaagta aagggagctt tagttcaaca gtcgatttct
     1021 taaaatcggc tttatttatg ggtgaaaatt acattaccgt tgtaaatccg cattttcagg
     1081 actctacccc ttctagagcg cctctcattg ttttagcgat taattattat tccccttatt
     1141 atttttttga aaatgttctt tgtaatataa ataatcaact ttatgttttg tggacattaa
     1201 ttgagtcgct aatattgcgg tgtaaattgt cgtcaatcct agtgctacta cggctaataa
     1261 atttaaatta cttttattaa tgctactgtt actattcatg aaagctcaac tcccttcttt
     1321 aaaattagat ttctaaatat caaagatttt tattcgaaca ccatcaattc tccccttgtt
     1381 ttagggtgga ggctggcaag ggggtttggg ggaagccccc atattgcgta gatccataag
     1441 gcaaaagaga aaatttatga acggaagctc tggcggtttt ggcggtataa tggctcgagc
     1501 aagccgattt cctgaattcc tcgttttagg tacgtcggct tggcgaaagt acagcaccgc
     1561 caaaatttcg gaatgcaagg agaaaagggt ggagattttt cggtggtctg tccgaaaaat
     1621 actacccgaa ccttgcgtgg agaaaagccc tttaggagat atagaaggga acacagaaaa
     1681 aatgttgata tatcaatgtt ttagttaagg taatgtgtca tagcttgtga cacgtatatg
     1741 tgtcatagct tgtgacacat agttgaatta agatgtgtca ttttttgctt gactatagtt
     1801 catttttgac ctatgatgtg tcataaatta tacatatagg tatcacagct gatgatacat
     1861 aacaagggag agaaggattc tatgccagca gaggtgcgta aattggacga gcagagaaaa
     1921 attaatgttg agttaacccc tgaacaactt caaatgcttc aaagacttat tgatcttgaa
     1981 cagcaagaaa acaaagacaa agagcaattt gaagagaaaa agaaaaatca taatttcatc
     2041 cagctttatc gtgacaacat gccagaatta agatggttaa tggcaaatca taacttcgca
     2101 agttctttat tattttttat cttggaacac atggataaca gaaatgcttt agcttgttct
     2161 tatgcagttt ttgaagatta ttttggcaaa tcaaggtcaa cagtttacag agcagtaaag
     2221 cttttagagg agaatggatt tttaaatgtt ttaaaaatgg gaacatcaaa cgtctatgta
     2281 gtgaacgaag accttgcttg gacagatggc aatgacaaaa agaaatttgc taaatacgat
     2341 ggtaaaatcc ttgtcagcaa aaaggaaaac aaggactttg aatacaaaag ccaatttgat
     2401 aaatttaaag cccttagaga acgtgaaaac ttaaaataaa gcgagtagat aactactcgc
     2461 ctttctttat ttcaagagca tatttgccct catttgaatc ttggtcaaca cttgtaaaaa
     2521 caccaatatc ttttattgga ataagagttc cagaacctaa atgaggtctc tttctatccc
     2581 tcataaaaag cattaattct ccctcttggt ctagtttctc caacttctca accaattgtt
     2641 tcaccttcat cagcgttcca tctccatccc tttttgcctt tgtggcttct ccattttttc
     2701 taaagagtcc tggactaatt tatcttgcct atcatccagg aacttgattg tacttcggtc
     2761 tacatcattc atcctcaaga acgtttttac tcggtcaagt tgagcgtgta aaactttgcc
     2821 caccctctct ttaaaaacag aaaactcctt gctgatctgc acgagttttc ccttcaattt
     2881 tccgttctct aatttcaatt cccgattctc attttggtaa gaccggttct caatttttag
     2941 agtgtccacc tgttgtttta actccttttt ctccttcaac tggtcattat atttattaac
     3001 gagtccattg tacttttcta tcaagtctcc atagttcttt acaatgtctg tttgcttcac
     3061 tttttgcagt gcttttttct ctgctaagga gtctaaattc tcttcaatat ctttgtagta
     3121 atagcgaatt ttatcttgtc caatttgcat atcagctttc agctgcgtaa cttctttttg
     3181 agtcttttcc aactctttct gcagattctc tgcttgtagc tgataagcca caccattggt
     3241 cgcataatcg actaggcttt tgtagtcttg ttccttcatt ccaacaagcc ctagaacctt
     3301 tttagcttcg atattcgcaa tcgatttcga tttatcatca atctcttgaa tatgccccat
     3361 tgtccgctca tatttctctt gggcttcctt ctccatgctt tgaaacgttt gagccttata
     3421 tttagccgtc tccacgtgtt tacggctcga cgagacccct ctttccaaat taaagtcatt
     3481 ttgcttcagg tactgattaa aatcgtcctg aagactcacc agttgctttt tctggccaaa
     3541 gaaatcttta gccgatagtc ggccatcctc cgttactgga acgaagccta cgtgcatgtg
     3601 aggggttttc tcgtcatagt gaacagtcgc atagattaaa ttcttttcac cgtatcgatc
     3661 agcaaggaac tcatgagccg tttcaaaata ccttttctgc tctttgggtg aaattttatc
     3721 gaagaattcc ttgtcagagg taattaagaa ttcagccagc ttaactgcat ctttacggat
     3781 cttttttcca gaggtcacat tgtcctcaat tacttggcta attttctcgt tgtaatcaat
     3841 ctgcctttga ccgtttatca agtcataatt cagatgagaa tcagctttat tgatgtcggg
     3901 attcgtttca ctttccctct ctctttggtt gtgaaattga atccctttta tcccatttga
     3961 tttcattttc tgcattcgga taatggagta actcatgggg tacgcaccct ttctgaaaat
     4021 cgatagatta atagaaagtg taacacacta cactttgcac gcaaagtttc atgtggtctg
     4081 cgacgctaaa agccaaacct tgggctttgc ccaaacccac tggggaactc ttccccagac
     4141 ccccttaatc gaactcccca acccctcaat ccttgagggg ctccctcgcc ggtaggcagc
     4201 tcaaaaggtg ttgccttttg agacaccaag agggtcttgg tgaagtgagt ctatcaactc
     4261 cttatcctcc cttcggtccg gtaccgttga cagacgccat acgggcctct ctcgcctttg
     4321 taagaggttg cccctagtaa aaacccttaa aaagcaaaaa agacgcttct aggggtagcg
     4381 tcagggaaat gcggatttac aacggtaagc atttgataca agtcttatga gacagtcaga
     4441 agtcatttaa aacgctaatt tcattatgtc tcaataaact ctactctatt ggaattgtga
     4501 agtgatacac ttaaactaac gtacgcaggt ttgttgaata aggacttcaa aaaataccct
     4561 taattaagct aaaaagtcct tctctttatt aagaaggact tttttatgtg ttattccatt
     4621 gtctcattca ttgtaatagt ttcgcttgct tcatgaccag attctccatg tcctggtcca
     4681 aacaaattca atattac
//
