LOCUS       NZ_AP022992             4987 bp    DNA     circular CON 14-MAR-2024
DEFINITION  Bacillus cereus strain 30052 plasmid p30052_6, complete sequence.
ACCESSION   NZ_AP022992
VERSION     NZ_AP022992.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203341
            Sequence Read Archive: DRR206381, DRR206405
            Assembly: GCF_021655315.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 516x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655315.1-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 04:23:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,137
            CDSs (total)                      :: 5,984
            Genes (coding)                    :: 5,659
            CDSs (with protein)               :: 5,659
            Genes (RNA)                       :: 153
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 211 of 325
            Pseudo Genes (incomplete)         :: 130 of 325
            Pseudo Genes (internal stop)      :: 52 of 325
            Pseudo Genes (multiple problems)  :: 52 of 325
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022992.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="30052"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="p30052_6"
                     /geo_loc_name="Japan:Tottori"
                     /collection_date="2016"
     gene            184..762
                     /locus_tag="L6499_RS30500"
                     /old_locus_tag="BC30052_p61"
     CDS             184..762
                     /locus_tag="L6499_RS30500"
                     /old_locus_tag="BC30052_p61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_205191062.1"
                     /translation="MEKSFRQMSQEAELKAKKREQILSDEELLKANEIQAKANVRGMKL
                     VPERTIKNRDRYVQLQQLNISYLHNLKYLTNAEKVLLFDMCPHVGIESNCIVDDVYEKV
                     QSPLTITEFASKIGRTRNNVSPIINSLIDKGILARSETGLDNNNVRAYSLFVNPNIMYS
                     GNRDSVNGTLIAMFKKVPKELKKLPVKMF"
                     /name="MarR family transcriptional regulator"
     gene            2453..3619
                     /locus_tag="L6499_RS30505"
                     /old_locus_tag="BC30052_p62"
     CDS             2453..3619
                     /locus_tag="L6499_RS30505"
                     /old_locus_tag="BC30052_p62"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_236805894.1"
                     /translation="MENTFTSKINGVYDWLKKQQITFVDQDKKAYKKNNSHSTWDTYCN
                     VMKTYEKWLQKEHGLKDITRAKPKHAEEYMQNLIDKYKRGEGSAYTLSKFPHALHSLQQ
                     SARESKVYRGLKLGNKADLIAMKNGEGIERKSSESKCLKANASDFDKVQNEIANSRSPQ
                     KEIIMNIHQIQRGVGCRIHEVVKMKGEDITYNQNGSATVYIKGKGGLERWVHVDDKKTI
                     ELLKEQTKDKKDGAYVFPLKNKEGNDKNLSSSMKLAKDVIRSAAERSGVDRDGKTYSTH
                     SARKVYAQERMNAYASMSQEQLQKELTKRIRNYPIDKYGQNRLKEKVDSELQEKRNKIT
                     REDKDEQKRLRKERDWTHKELCEFMVSIDTGHFRTNIMRYYCDYPKDK"
                     /name="tyrosine-type recombinase/integrase"
     gene            4392..4772
                     /locus_tag="L6499_RS30510"
                     /old_locus_tag="BC30052_p63"
     CDS             4392..4772
                     /locus_tag="L6499_RS30510"
                     /old_locus_tag="BC30052_p63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_205191064.1"
                     /translation="MNKKISIEEMTQEMKEKYTLTLEEAHKMLLEHEVTKSKNVQVTMR
                     WIRDGKIDGVLIGKGRVETRKWLVSEESLLEFIDFERLTLGDYKAMKKELQELRGFKFE
                     VENKPTRTRKKTTPKDKTEDKK"
                     /name="hypothetical protein"
     gene            4815..4949
                     /locus_tag="L6499_RS30650"
                     /old_locus_tag="BC30052_p64"
     CDS             4815..4949
                     /locus_tag="L6499_RS30650"
                     /old_locus_tag="BC30052_p64"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272884484.1"
                     /translation="MQKDKENLKDFLQEKDEEFNKKIEMYDKQIEKLKHEILDNECDE"
                     /name="hypothetical protein"
ORIGIN
        1 gaaaagcaag tggagataaa gaaaaaggag tcatgggtgc gaccaacacc aatgactccg
       61 aaggattttt agttcccgaa tgaactattc aaaaatataa agttatcata aatagttttc
      121 gaaaaaaagg caagaattcc ttctcttttc tccctaccaa ttttgtattt tggaggggtt
      181 agcatggaaa aaagttttag acaaatgagt caagaagctg aattgaaggc taaaaaacgt
      241 gaacagattt taagtgatga agaattatta aaagctaatg aaatacaagc aaaagcgaat
      301 gttcgtggta tgaaattagt tcctgaaaga acgattaaaa atcgtgatag atacgtacag
      361 ttacagcaat tgaatatatc gtatttacat aatttaaaat atcttacgaa tgctgaaaaa
      421 gtattattat ttgatatgtg tcctcatgtt ggaattgaat caaattgtat tgttgatgat
      481 gtttatgaaa aggttcaatc acctttgacg ataacagaat ttgcttcaaa gattggaaga
      541 acgagaaata atgttagtcc aattattaat agtttgattg ataaaggaat tttagctcgg
      601 tcagaaacag ggctagataa taataatgtt cgggcttatt cattgtttgt taatccaaat
      661 attatgtatt caggtaatcg agattcagtt aatggaactt tgattgcgat gtttaaaaaa
      721 gttccaaaag aacttaaaaa attacctgtg aaaatgttct aagacctaga gaaatctagg
      781 ttttttgttg ttcttaaaat caagacgggg tattatgaaa aattcgtagt tcttaaaatt
      841 aagacggaag cgtcttaaaa ataagaacta cgaaaaatct cgaaaccatt gatatatatg
      901 gtttttttta atttaaaaaa gtcttttctt cttactcttg tattattgtt tttttctttc
      961 gatttttaat agattctcat gccttatatt tactacgttt ttaaagaaaa accgctctcg
     1021 aatctatcaa atgacaggta gtattttctt tcgctttgct acagaaaatc tccaccttca
     1081 agcatttgac agaaacgagt cgtcacgtgg gtttgcatca ccaaaccgat acaccaaaaa
     1141 ggagataaat aggttatcga tttggatgag aaagtatacg tgacgaacgc tctgagcgta
     1201 catatgcggt ttattttttt atagtgaaac gttggggcca gccccaacac cccgcccaaa
     1261 acctcaaccc aaaaagtaag ggaaaaccgt ttccaaatcg gaaatgacta cttgtccttt
     1321 ttcctaagga ggaacagtct tgtccatgtt tgtccaatcg taagattggg ttgcttccac
     1381 caatcggaac gtttcctcga ttgtcctaga aactcaagga ttttaaacga aattaaatta
     1441 aattatttta attgaaaaat ggaaaaaact ggttatgatt agaaggaact taaaaaccgc
     1501 caatggaatt tggaattgtt tgatgaaacc tttcggttcg ctgagagaga gtaaaaaaac
     1561 aagaaagcag acaatcaaaa gagaatccct aaacgtttta tatgcgtaaa acgtactgta
     1621 aggaagatgt caaaaagaca acttctgaaa acgattgggg gtgaatatga atatatcaac
     1681 ctacaagtaa taccatgtag tacaagtaag gcaacagtct ctgttgcaac caaatattcc
     1741 aacactcaca atcataactt tagacaattt cgttttaggg tcgaatcaag agaagaaacg
     1801 cacatcaaat acaattaaaa aaaggaggag atataaggag aatagtagat gaatgttgat
     1861 tgaaagagat gatggatagt gtttcaaaac gtattaaaaa aatgttttat gtaacaaaaa
     1921 tcaaatgtaa caaaatgcaa taaacgtata tctgagcgtt atcagaccga tttgcaaact
     1981 gtcactctgt ggctgactga acatgagaaa gtgtcgagaa gcgacaactt gttttgaggt
     2041 tttcaccgaa ggtgaaaagc gataaaacaa gatggagcgt tgcacgaggt tggcgtgagg
     2101 tttgccgaag gcaaagcgaa cgacaagcga gggcatcgag taaaactttc gaagggagaa
     2161 caactgaata tgggatatgg ggttatgttc gagggggttc tatcgtattt ttttcgaaga
     2221 aaaacatagg atagaaccac cctcggtggt tagggaggag tgtttttttg catggtttta
     2281 tggcaaaaaa atacgacttg cttttgaatt taggttttta tttttaaaac cacatttaaa
     2341 aaccattttt atataacagc aaccgcaagg cggttggtgg gtgtggattg tgccttttaa
     2401 gagccgtaac atgccctaga atcaattttt actctttggg gggtgtcttt gtatggaaaa
     2461 cacttttaca agcaaaataa atggtgttta cgattggtta aaaaaacaac aaattacttt
     2521 tgttgaccaa gataaaaaag cgtacaagaa aaataactct cattctactt gggatacgta
     2581 ttgtaacgtg atgaaaacct atgagaagtg gcttcaaaag gaacatgggc taaaagatat
     2641 cacacgagcg aaacccaaac atgctgaaga atatatgcag aatttgattg ataaatacaa
     2701 gcgtggcgag ggttcggcat acacgctttc taagtttcct catgctttac attcgttgca
     2761 acaatcagca agagaatcaa aggtttatcg tggtctgaag ctaggaaaca aggcggattt
     2821 gattgcaatg aagaatggcg aaggaattga aagaaaaagt agtgaatcta agtgtttgaa
     2881 ggcaaatgca agtgattttg ataaagtgca aaatgagatt gcaaattctc gttctccaca
     2941 aaaagaaatc atcatgaata ttcatcaaat acaacgtggt gtgggttgtc gtattcatga
     3001 agttgtcaaa atgaaaggtg aagatatcac atacaatcaa aacggttcag caacggttta
     3061 tatcaaggga aaaggcggtt tggaacgttg ggttcatgta gacgataaaa agacgataga
     3121 gctattaaaa gagcaaacaa aagataaaaa agatggtgca tatgtatttc cactgaaaaa
     3181 caaagaaggt aatgataaaa atttgtcatc atctatgaag ttagcaaaag acgtgattcg
     3241 ctccgcagcg gagcgttcag gtgtagatag agatggaaaa acatatagca cacattcagc
     3301 aagaaaagtg tatgcacaag agcgtatgaa tgcttatgcg agtatgtctc aagagcaatt
     3361 gcaaaaggaa ttaacaaagc gaattcgtaa ttatccaata gataaatatg gtcaaaatag
     3421 attaaaagaa aaagtggata gtgaattgca agaaaaacgc aataaaatca cgagagaaga
     3481 caaggacgaa caaaaaagat taagaaaaga acgtgattgg actcataaag aattatgcga
     3541 atttatggtg agcatcgata cagggcattt tagaacgaat attatgagat attactgtga
     3601 ttatccgaag gataaatgat gttgtttttt ggtttgtttt agcgaggata atcgggttta
     3661 atcgctattt agggaagctt agcggagcat ggtatggagt ggcacgttgg cgaagaaaca
     3721 cagggtggag gctcgttgta aagtccgtag tgtaacggag agcgaagcgg tgactttaca
     3781 atgagaatcc ctgtgttagc ctacgtgggt tgccaaccca aacatgcgga caaagggaag
     3841 aagggaaaaa aatagggaga atcctcgcaa aaccgcataa aatggggttt tttatttttt
     3901 tctcaaatag atagattttt aagtaaattt atttaatagt aaatttactt aaataataaa
     3961 tgtattataa agtaaattta atagatttac atgaaagtaa aactagtata taaaaataca
     4021 tttactcaaa agtaaatgta actatattta tacatgtatt tttaagtaaa aggattcgct
     4081 aagactctcc taaactcctt tgttttcaag gttttagagt ttgcgagtac atgtataaga
     4141 aagtaaagtc atggctaata ttatatttac tcaagagtaa aattaataaa aatattagat
     4201 ttactaaagg atatatttac ttttgattga atgtaattta tagtaaatat aaatcaaaat
     4261 aaaaatattt ccaattaaat ttattgtcta gtacatttat tttttaatag atgtattgta
     4321 taataaatat aagagttaat acatgtaatc aaaaatacaa atacttaaaa atacatttag
     4381 ggggaattat gatgaataag aaaatctcaa ttgaagaaat gacacaagaa atgaaggaaa
     4441 aatacacgct aacactagaa gaagcacata agatgttatt ggaacatgaa gtaacaaaga
     4501 gtaagaatgt acaagtaaca atgcgttgga ttcgtgacgg taaaattgat ggtgtgttga
     4561 ttggaaaagg tcgtgtggaa actcgcaaat ggttggtaag tgaagaaagc ctgttagaat
     4621 tcattgattt tgaacgccta acattagggg attataaagc aatgaaaaag gaattacagg
     4681 aactacgagg gtttaaattc gaggtagaaa ataaaccaac gagaacacgt aaaaagacga
     4741 cacctaagga taaaacagaa gataagaaat aacaggtgga ctattccacc tcgttatttt
     4801 aaagaggtga gaatatgcaa aaagataagg aaaatctgaa agatttctta caagaaaaag
     4861 atgaagaatt taacaaaaaa atagaaatgt atgataaaca aatagagaag ttaaagcatg
     4921 aaatactaga caatgaatgt gatgagtgat aggtttttaa caaggtttta cgctaatttt
     4981 gttgaaa
//
