LOCUS       NZ_AP022985             4697 bp    DNA     circular CON 11-MAY-2024
DEFINITION  Bacillus cereus strain 30048 plasmid p30048_4, complete sequence.
ACCESSION   NZ_AP022985
VERSION     NZ_AP022985.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203340
            Sequence Read Archive: DRR206380, DRR206404
            Assembly: GCF_021655295.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 550x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655295.1-RS_2024_05_11
            Annotation Date                   :: 05/11/2024 05:30:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,910
            CDSs (total)                      :: 5,758
            Genes (coding)                    :: 5,457
            CDSs (with protein)               :: 5,457
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 196 of 301
            Pseudo Genes (incomplete)         :: 119 of 301
            Pseudo Genes (internal stop)      :: 44 of 301
            Pseudo Genes (multiple problems)  :: 49 of 301
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022985.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="30048"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="p30048_4"
                     /geo_loc_name="Japan:Tottori"
                     /collection_date="2016"
     gene            join(4552..4697,1..406)
                     /locus_tag="L6480_RS29415"
     CDS             join(4552..4697,1..406)
                     /locus_tag="L6480_RS29415"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_236784620.1"
                     /translation="MSISKHANKKKNILIAASEIVKEEGVVKLTLEAVAQRAGVSKGGL
                     LYHFPSKEALIKGMVEEWTNNYFECINTLVNNDDDNAIGKWNRAYLKSTFSDLENNNLN
                     SALMAAMFINPDLLDEFRQRYDILHTKLITDGIDPVKITITRLSIDGLWFSEIFGMAPL
                     NEELKTQVFDELINMIQEDE"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            406..783
                     /gene="qacH"
                     /locus_tag="L6480_RS29420"
                     /old_locus_tag="BC30048_p41"
     ARG             407..780
                     /name="qacH"
                     /gene="qacH"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacH"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.2"
                     /coverage="100.0"
     gene            1398..2744
                     /gene="mobV"
                     /locus_tag="L6480_RS29425"
                     /old_locus_tag="BC30048_p42"
     CDS             1398..2744
                     /gene="mobV"
                     /locus_tag="L6480_RS29425"
                     /old_locus_tag="BC30048_p42"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_053082646.1"
                     /translation="MSYSIIRMQKMKSNGIKGIQFHNQRERESETNPDINKADSHLNYD
                     LINGQRQIDYNEKISQVIEDNVTSGKKIRKDAVKLAEFLITSDKEFFDKISPKEQKRYF
                     ETAHEFLADRYGEKNLIYATVHYDEKTPHMHVGFVPVTEDGRLSAKDFFGQKKQLVSLQ
                     DDFNQYLKQNDFNLERGVSSSRKHVETAKYKAQTFQSMEKEAQEKYERTMGHIQEIDDK
                     SKSIANIEAKKVLGLVGMKEQDYKSLVDYATNGVAYQLQAENLQKELEKTQKEVTQLKA
                     DMQIGQDKIRYYYKDIEENLDSLAEKKALQKVKQTDIVKNYGDLIEKYNGLVNKYNDQL
                     KEKKELKQQVDTLKIENRSYQNENRELKLENGKLKGKLVQISKEFSVFKERVGKVLHAQ
                     LDRVKTFLRMNDVDRSTIKFLDDRQDKLVQDSLEKMEKPQRQKGMEMER"
                     /name="mobV"
     gene            2744..2941
                     /locus_tag="L6480_RS29430"
                     /old_locus_tag="BC30048_p43"
     CDS             2744..2941
                     /locus_tag="L6480_RS29430"
                     /old_locus_tag="BC30048_p43"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048558833.1"
                     /translation="MKVKQLVEKLEKLDQEGELMLFMRDRKRPHLGSGTLIPIKDIGVF
                     TSVDQDSNEGKYALEIKKGE"
                     /name="hypothetical protein"
     gene            complement(2955..3512)
                     /locus_tag="L6480_RS29435"
                     /old_locus_tag="BC30048_p44"
     CDS             complement(2955..3512)
                     /locus_tag="L6480_RS29435"
                     /old_locus_tag="BC30048_p44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016125306.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_048558832.1"
                     /translation="MPAEVRKLDEQRKINVELTPEQLQMLQRLIDLEQQENKDKEQFEE
                     KKKNHNFIQLYRDNMPELRWLMANHNFASSLLFFILEHMDNRNALACSYAVFEDYFGKS
                     RSTVYRAVKLLEENGFLNVLKMGTSNVYVVNEDLAWTDGNDKKKFAKYDGKILVSKKEN
                     KDFEYKSQFDKFKALRERENLK"
                     /name="replication/maintenance protein RepL"
     ARG             407..783
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="61.68"
                     /coverage="116.82"
     Typing          complement(2956..3512)
                     /name="rep_cluster_1925"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.818"
                     /coverage="100.0"
                     /evalue="1.87e-127"
ORIGIN
        1 tcctagcaag gaagcattaa ttaaaggaat ggtggaagaa tggacaaata attatttcga
       61 atgcattaat acgttagtta ataacgacga tgataacgca ataggaaaat ggaatagggc
      121 ttatcttaaa tctacatttt ccgatttaga aaacaataat ctaaattccg cactaatggc
      181 tgctatgttt attaatccag atttattaga tgaattccgg cagagatacg atattttgca
      241 tacaaagtta ataactgatg gaattgatcc tgtcaaaata acaattacaa gattatccat
      301 agatggtctt tggttttcag aaatatttgg aatggcacca ttaaatgaag aactaaaaac
      361 acaagttttt gatgaactaa taaacatgat tcaggaggac gaataatgtc atatctatat
      421 ttagcacttg caatagtggg ggagattatt ggctcttccc tattaaaagc atctgaaggc
      481 ttttcaaaac tctatcctac aataggggta ataatagctt tcgttggttg ttttttcttt
      541 ttatccttgt cgctaaaaac aatcccctta aacactgcat atgcattatg ggcaggttta
      601 ggacttgttt tgactacagt aatttcagtt ttagtttgga aggaaaaact taacatggct
      661 agcattgcag gaataacact gattgttgta ggagttgtaa tattgaattt gtttggacca
      721 ggacatggag aatctggtca tgaagcaagc gaaactatta caatgaatga gacaatggaa
      781 taacacataa aaaagtcctt cttaataaag agaaggactt tttagcttaa ttaagggtat
      841 tttttgaagt ccttattcaa caaacctgcg tacgttagtt taagtgtatc acttcacaat
      901 tccaatagag tagagtttat tgagacataa tgaaattagc gttttaaatg acttctgact
      961 gtctcataag acttgtatca aatgcttacc gttgtaaatc cgcatttccc tgacgctacc
     1021 cctagaagcg tcttttttgc tttttaaggg tttttactag gggcaacctc ttacaaaggc
     1081 gagagaggcc cgtatggcgt ctgtcaacgg taccggaccg aagggaggat aaggagttga
     1141 tagactcact tcaccaagac cctcttggtg tctcaaaagg caacaccttt tgagctgcct
     1201 accggcgagg gagcccctca aggattgagg ggttggggag ttcgattaag ggggtctggg
     1261 gaagagttcc ccagtgggtt tgggcaaagc ccaaggtttg gcttttagcg tcgcagacca
     1321 catgaaactt tgcgtgcaaa gtgtagtgtg ttacactttc tattaatcta tcgattttca
     1381 gaaagggtgc gtaccccatg agttactcca ttatccgaat gcagaaaatg aaatcaaatg
     1441 ggataaaagg gattcaattt cacaaccaaa gagagaggga aagtgaaacg aatcccgaca
     1501 tcaataaagc tgattctcat ctgaattatg acttgataaa cggtcaaagg cagattgatt
     1561 acaacgagaa aattagccaa gtaattgagg acaatgtgac ctctggaaaa aagatccgta
     1621 aagatgcagt taagctggct gaattcttaa ttacctctga caaggaattc ttcgataaaa
     1681 tttcacccaa agagcagaaa aggtattttg aaacggctca tgagttcctt gctgatcgat
     1741 acggtgaaaa gaatttaatc tatgcgactg ttcactatga cgagaaaacc cctcacatgc
     1801 acgtaggctt cgttccagta acggaggatg gccgactatc ggctaaagat ttctttggcc
     1861 agaaaaagca actggtgagt cttcaggacg attttaatca gtacctgaag caaaatgact
     1921 ttaatttgga aagaggggtc tcgtcgagcc gtaaacacgt ggagacggct aaatataagg
     1981 ctcaaacgtt tcaaagcatg gagaaggaag cccaagagaa atatgagcgg acaatggggc
     2041 atattcaaga gattgatgat aaatcgaaat cgattgcgaa tatcgaagct aaaaaggttc
     2101 tagggcttgt tggaatgaag gaacaagact acaaaagcct agtcgattat gcgaccaatg
     2161 gtgtggctta tcagctacaa gcagagaatc tgcagaaaga gttggaaaag actcaaaaag
     2221 aagttacgca gctgaaagct gatatgcaaa ttggacaaga taaaattcgc tattactaca
     2281 aagatattga agagaattta gactccttag cagagaaaaa agcactgcaa aaagtgaagc
     2341 aaacagacat tgtaaagaac tatggagact tgatagaaaa gtacaatgga ctcgttaata
     2401 aatataatga ccagttgaag gagaaaaagg agttaaaaca acaggtggac actctaaaaa
     2461 ttgagaaccg gtcttaccaa aatgagaatc gggaattgaa attagagaac ggaaaattga
     2521 agggaaaact cgtgcagatc agcaaggagt tttctgtttt taaagagagg gtgggcaaag
     2581 ttttacacgc tcaacttgac cgagtaaaaa cgttcttgag gatgaatgat gtagaccgaa
     2641 gtacaatcaa gttcctggat gataggcaag ataaattagt ccaggactct ttagaaaaaa
     2701 tggagaagcc acaaaggcaa aaagggatgg agatggaacg ctgatgaagg tgaaacaatt
     2761 ggttgagaag ttggagaaac tagaccaaga gggagaatta atgcttttta tgagggatag
     2821 aaagagacct catttaggtt ctggaactct tattccaata aaagatattg gtgtttttac
     2881 aagtgttgac caagattcaa atgagggcaa atatgctctt gaaataaaga aaggcgagta
     2941 gttatctact cgctttattt taagttttca cgttctctaa gggctttaaa tttatcaaat
     3001 tggcttttgt attcaaagtc cttgttttcc tttttgctga caaggatttt accatcgtat
     3061 ttagcaaatt tctttttgtc attgccatct gtccaagcaa ggtcttcgtt cactacatag
     3121 acgtttgatg ttcccatttt taaaacattt aaaaatccat tctcctctaa aagctttact
     3181 gctctgtaaa ctgttgacct tgatttgcca aaataatctt caaaaactgc ataagaacaa
     3241 gctaaagcat ttctgttatc catgtgttcc aagataaaaa ataataaaga acttgcgaag
     3301 ttatgatttg ccattaacca tcttaattct ggcatgttgt cacgataaag ctggatgaaa
     3361 ttatgatttt tctttttctc ttcaaattgc tctttgtctt tgttttcttg ctgttcaaga
     3421 tcaataagtc tttgaagcat ttgaagttgt tcaggggtta actcaacatt aatttttctc
     3481 tgctcgtcca atttacgcac ctctgctggc atagaatcct tctctccctt gttatgtatc
     3541 atcagctgtg atacctatat gtataattta tgacacatca taggtcaaaa atgaactata
     3601 gtcaagcaaa aaatgacaca tcttaattca actatgtgtc acaagctatg acacatatac
     3661 gtgtcacaag ctatgacaca ttaccttaac taaaacattg atatatcaac attttttctg
     3721 tgttcccttc tatatctcct aaagggcttt tctccacgca aggttcgggt agtatttttc
     3781 ggacagacca ccgaaaaatc tccacccttt tctccttgca ttccgaaatt ttggcggtgc
     3841 tgtactttcg ccaagccgac gtacctaaaa cgaggaattc aggaaatcgg cttgctcgag
     3901 ccattatacc gccaaaaccg ccagagcttc cgttcataaa ttttctcttt tgccttatgg
     3961 atctacgcaa tatgggggct tcccccaaac ccccttgcca gcctccaccc taaaacaagg
     4021 ggagaattga tggtgttcga ataaaaatct ttgatattta gaaatctaat tttaaagaag
     4081 ggagttgagc tttcatgaat agtaacagta gcattaataa aagtaattta aatttattag
     4141 ccgtagtagc actaggattg acgacaattt acaccgcaat attagcgact caattaatgt
     4201 ccacaaaaca taaagttgat tatttatatt acaaagaaca ttttcaaaaa aataataagg
     4261 ggaataataa ttaatcgcta aaacaatgag aggcgctcta gaaggggtag agtcctgaaa
     4321 atgcggattt acaacggtaa tgtaattttc acccataaat aaagccgatt ttaagaaatc
     4381 gactgttgaa ctaaagctcc ctttacttta agtacatttc ttcacaatct ttagattaat
     4441 ttattggatt atttttatgt gaaagagtga tattaaataa gagctttaat tcgattgaca
     4501 aaaaaaccgt ccagaaggta tattatatgt atctaagtta tggaggttat tatgtctatt
     4561 tcaaagcatg ctaataagaa aaaaaatata ttgattgctg catctgaaat tgtaaaagaa
     4621 gaaggagttg tcaaactcac attagaggca gtcgcccaaa gagctggcgt gagtaaaggt
     4681 ggattattat accattt
//
