LOCUS       NZ_AP022984             7635 bp    DNA     circular CON 11-MAY-2024
DEFINITION  Bacillus cereus strain 30048 plasmid p30048_3, complete sequence.
ACCESSION   NZ_AP022984
VERSION     NZ_AP022984.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203340
            Sequence Read Archive: DRR206380, DRR206404
            Assembly: GCF_021655295.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7635)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 550x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655295.1-RS_2024_05_11
            Annotation Date                   :: 05/11/2024 05:30:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,910
            CDSs (total)                      :: 5,758
            Genes (coding)                    :: 5,457
            CDSs (with protein)               :: 5,457
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 196 of 301
            Pseudo Genes (incomplete)         :: 119 of 301
            Pseudo Genes (internal stop)      :: 44 of 301
            Pseudo Genes (multiple problems)  :: 49 of 301
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022984.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="30048"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="p30048_3"
                     /geo_loc_name="Japan:Tottori"
                     /collection_date="2016"
     gene            339..539
                     /locus_tag="L6480_RS29370"
                     /old_locus_tag="BC30048_p31"
     CDS             339..539
                     /locus_tag="L6480_RS29370"
                     /old_locus_tag="BC30048_p31"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139252680.1"
                     /translation="MNIRISVVLPYGYSYSDSNRKCDCINVFGVFERIEKAEALPVKQK
                     NMFVEEVVISLQENSHRSVGR"
                     /name="hypothetical protein"
     gene            950..2155
                     /gene="mobV"
                     /locus_tag="L6480_RS29375"
                     /old_locus_tag="BC30048_p32"
     CDS             950..2155
                     /gene="mobV"
                     /locus_tag="L6480_RS29375"
                     /old_locus_tag="BC30048_p32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033080.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000033078.1"
                     /translation="MSKFMVCHMQKFKMTDVKGLQIHNQREKESHSNSDIIQERTEQNY
                     DLIHDKRKVDYKKFIQNRIDNGVVSNRAIRKDAVVCCSFMISASPDYMNSLSQAEQRRF
                     FEESVCFLKARYGEENFVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFKNKSELHDL
                     QDKIYEHVKEKGFDIERGVSSDRKHLSTQRFKAVSLQQEIEKLEQEKKEIDSRLYDLAS
                     SLDQAKSVDEIPVKEKGGFIRSKMVEIASEDFDSIKSLAKSSESLRNENRRLKNEKIKI
                     EREKDDLYKGQRFLERQVTDLKRENRGLKEANDFLKKTLERVKEMYKEKLPELAGVIGY
                     VKGSILDKMNRKFLKRHFAGDDEVKGAQKFLNHKQEHEEQQKRLKQVRRSQQKNWDQGL
                     ER"
                     /name="mobV"
     gene            2187..2468
                     /locus_tag="L6480_RS29380"
                     /old_locus_tag="BC30048_p33"
     CDS             2187..2468
                     /locus_tag="L6480_RS29380"
                     /old_locus_tag="BC30048_p33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002026684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_003273526.1"
                     /translation="MEYFHSYGVKFIGTVKELAKKNNLTIEETCRILELTMKIDDYDRK
                     DEQLAGIGELIKEMNDQIMGLSGGLRAISERLRDMNENVVLVSLEDKE"
                     /name="hypothetical protein"
     gene            complement(2630..3031)
                     /locus_tag="L6480_RS29385"
                     /old_locus_tag="BC30048_p34"
     CDS             complement(2630..3031)
                     /locus_tag="L6480_RS29385"
                     /old_locus_tag="BC30048_p34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158318.1"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="WP_000158319.1"
                     /translation="MTIYITETQAIFINETLIDMYSPNEQRGVKDTGLLQSAIYRPQQT
                     VAQEDAYPTIFHKATALFESLAKNHAFYNANKRTALACLEMFLLYNEYELKMSEQESSD
                     FTVNVVEQRLSFEEILKIIKENSSKLPLN"
                     /name="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(3069..3353)
                     /locus_tag="L6480_RS29390"
                     /old_locus_tag="BC30048_p35"
     CDS             complement(3069..3353)
                     /locus_tag="L6480_RS29390"
                     /old_locus_tag="BC30048_p35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126403.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_000126403.1"
                     /translation="MTANTRHMETQKIERKVTKIGNSLGLTLPQEFYTTLNIKQGDVVS
                     LEINEENEQIVIKKSKKISMPEDLNPKFLESLNRTINKFDGAFKDLVNR"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            4224..5267
                     /locus_tag="L6480_RS29395"
                     /old_locus_tag="BC30048_p36"
     CDS             4224..5267
                     /locus_tag="L6480_RS29395"
                     /old_locus_tag="BC30048_p36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162903.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_000162904.1"
                     /translation="MTKKNQDEITGKLQPKKKQNIRIYEFIEKKMSESGRELFKSCSTF
                     NEFVATKDKKKKKRVKGNDCKNRFCPICAWRKAGKDAVKIATMMEAIKIEEKKEFLFLT
                     LTTPNIKADTVKSEIDRFNKAFNKLFKRRNIQRSIKGYIRKLEMTYDKERFITEEMYNN
                     KKRKAYYDSRGLKVDDHNPNYDTYNPHFHVLLCVEKNYFKRKELYIKQEEWLEMWREVT
                     DMPEITQVHIQKVELIREGNAVAEVAKYSAKDYEMSVSQDVFDVFYLALKGRQLIVYGG
                     LLKDYAKKYEDGELDKYKSTDKNEYYYRLIAMWNKDLMKFEQEYQELTESEKQEYNGHL
                     IDEMEVE"
                     /name="protein rep"
     gene            complement(6272..6535)
                     /locus_tag="L6480_RS29400"
                     /old_locus_tag="BC30048_p37"
     CDS             complement(6272..6535)
                     /locus_tag="L6480_RS29400"
                     /old_locus_tag="BC30048_p37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874966.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPNELIKIGISIDDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     gene            complement(6549..6836)
                     /locus_tag="L6480_RS29405"
                     /old_locus_tag="BC30048_p38"
     CDS             complement(6549..6836)
                     /locus_tag="L6480_RS29405"
                     /old_locus_tag="BC30048_p38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236784618.1"
                     /translation="MAYIISKKVKGNIYFYVAQYVGTQPYYSKQYKYKCIYAIGNQKIA
                     LERIAMWLLDNNRIPKELLEIGVSINDVKYWYEKVEKTLQNYSLTNHKNS"
                     /name="hypothetical protein"
     gene            7194..7421
                     /locus_tag="L6480_RS29410"
                     /old_locus_tag="BC30048_p39"
     CDS             7194..7421
                     /locus_tag="L6480_RS29410"
                     /old_locus_tag="BC30048_p39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869766.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000869766.1"
                     /translation="MKVVNNFRVIMAVKNIDNVAELVRMTGVSRNSINKLWRNDNVSSL
                     RIDTLLPICEKLNVKLSDLIEYIPDDSETK"
                     /name="helix-turn-helix transcriptional regulator"
     Typing          4090..5267
                     /name="rep_cluster_617"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          951..2152
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aacatcttgg aagatgagag gtaggcagat atttcgtgat tttgtttaac tttatgtgtg
       61 attatcgtaa tgcagtaaaa tgaaatggtg gaattgatga gtaatgaaaa aagagttcct
      121 atttgtgggg ctcttttttt atttgtcatg gtgtatgcaa agcacatgca gaatacatac
      181 taaacgtttt taaacgtata gaggacgcat actttaggga aaatcgcttc cctgaagtag
      241 agcatgagaa acccaatagg cacagtctat ttcaccactt tttgtacaaa gtgggattcg
      301 gtttccttat gctctttttt atttgaatgg gtctaggaat gaatatacga atatctgtag
      361 ttttacccta tggctattct tatagtgatt ctaatagaaa atgtgattgt attaatgttt
      421 ttggggtgtt tgaaagaata gaaaaagctg aagctcttcc tgtaaaacaa aaaaatatgt
      481 ttgtagagga agtagttatt tcactacaag aaaattccca tagatcggtg gggcggtgaa
      541 tataaaaaga gactttttaa tatatggatg tcagatttta aaagggggag gagtatttat
      601 ggtacctgtc aacggtaagc ggacggaatg aagcgatagc gaaatgatgg agcattaagg
      661 agttgatagg taacttcgac caagaccctc ttgttcttcc caaaaccatg ttttggggat
      721 gcctaccggc gagggagtcc ctcaaggatt gaggggttgg ggagttcgat tgaaaggggt
      781 caaggggaag agtttccctt gtgggtttgg gcaaagccca taattctttt gccatgcctg
      841 tcatggctcg gcggtcgccg aaatgtcccg tagggcacac gtgtttacgc agtaaactgt
      901 agtgtgctac agtttacatg gaactacgac cgaagaagga gcgatgccca tgtcaaaatt
      961 catggtttgt catatgcaaa aattcaagat gacagatgtg aaaggattac aaatacataa
     1021 tcagcgtgaa aaagaaagtc attcaaattc agacattatt caagaacgaa cggaacagaa
     1081 ttatgatctt attcatgata aaagaaaagt ggattataag aaattcattc aaaatcgaat
     1141 tgataatggt gttgtatcta atcgtgcgat tcggaaagat gctgttgttt gttgttcgtt
     1201 tatgatttcg gctagtcctg attatatgaa ttctttatct caagcggaac agagacgatt
     1261 ctttgaagag tctgtttgtt ttttaaaggc gagatatgga gaagagaact ttgtatatgc
     1321 ttcagtacat gttgatgaaa aaacacctca tatgcatgtg ggcatggttc cggttaatga
     1381 aaaacaaaaa ctatcagcgt attcattttt taaaaacaaa tcagagcttc atgatctaca
     1441 agataaaatc tatgaacatg tgaaggaaaa gggatttgat atagagcgtg gtgtttctag
     1501 cgacaggaaa catttatcga cgcagcggtt taaagcggtg agtttgcaac aagaaataga
     1561 gaagttagag caggaaaaga aagaaataga tagtcgtctt tatgatttag catcgtcttt
     1621 agatcaagcg aaaagtgtag atgagattcc agtcaaagaa aaaggcgggt tcatacgctc
     1681 taaaatggtc gagattgctt cagaagactt cgatagtata aaatcattag ctaagtcttc
     1741 tgagtctctt agaaacgaaa atagacgcct taaaaatgaa aagattaaga ttgaacgtga
     1801 aaaagatgat ttatataagg ggcaacgttt tttagaaagg caagtaacag acttgaaaag
     1861 agagaatcga gggttgaagg aagcaaatga ttttcttaag aaaacgttgg agcgagtgaa
     1921 agagatgtat aaggagaagc ttcctgaact cgcaggggtg attggttatg ttaaggggag
     1981 tatactggac aagatgaatc gcaagttttt aaaacgacat tttgctggtg atgatgaagt
     2041 aaagggtgct caaaagttct tgaatcacaa acaagagcat gaagaacaac aaaagagatt
     2101 aaaacaagta cgaagaagcc aacagaaaaa ttgggaccag ggattggaga gatagtgtgg
     2161 gaacattatt taatcaacaa ccaaggatgg aatattttca tagttatgga gttaagttta
     2221 taggtactgt aaaggaattg gctaagaaaa ataatttaac gattgaagag acatgtagga
     2281 ttttggaatt gacgatgaaa attgatgatt atgatcgtaa ggatgaacaa ttagctggaa
     2341 ttggagaact tatcaaggaa atgaatgatc aaattatggg acttagtgga ggtctgagag
     2401 ctataagtga acgtttgcgt gatatgaatg aaaatgttgt attagtatct ttggaagata
     2461 aagaataatg gcatgttagg aggctctgtg tgagtctctt ttttctttag atgtgtagcg
     2521 ttagcggaac ttgttttgca taatatagcg aggattgtag agccttgtat aacatccttg
     2581 aagatagttt gtttaataaa aaagacgtgg ttaaatccca cgtctttttc taatttaaag
     2641 gtaattttga tgagttttct tttataattt ttaagatttc ttcaaaagat aatctctgtt
     2701 ctactacatt aacagtgaaa tctgaagatt cttgttcgga catttttaat tcgtactcat
     2761 tgtataacag aaacatttct aggcaagcta atgccgttct tttgtttgca ttatagaatg
     2821 cgtggttttt agccaatgat tcaaataatg cagtggcttt gtggaagata gtcggatatg
     2881 catcctcttg tgctactgtt tgttgtggac ggtatattgc tgattgtaat aaccctgtat
     2941 ctttaacacc tctttgttca ttaggtgaat acatgtcgat aagtgtttca ttgataaaaa
     3001 tagcttgagt ttctgttata taaattgtca tttcggtttt cctactttcc tccacgcctg
     3061 aaggataatt atctgtttac caaatcttta aatgcaccgt caaatttatt gatggttctg
     3121 tttaacgatt ccaagaactt aggattgaga tcctctggca tagaaatttt tttggatttt
     3181 tttataacga tttgttcgtt ttcttcgtta atttctaaac ttacaacgtc accttgtttg
     3241 atatttagag tagtgtaaaa ttcttgcggt aatgttaaac ctaaagaatt gccgatttta
     3301 gtaactttac gttcgatttt ttgtgtttcc atgtggcgag tgtttgctgt catttggact
     3361 tcctccttgg aatttgccac actatttttt gtcgtagtct aattgccact ttgtgtttgt
     3421 attgagtttt tcgaattata tagtgtgatt ttatgtttgt tttgtttgtt tgcttttggt
     3481 tttttcggtc ttcttagttt tctcttttgt ttgttgtgtt tagtctttct gtattattat
     3541 gacgtttagt tagaatgtta taacagttat aacgtattaa ccgtcaatac aattatactc
     3601 atttttagaa atatgtaaac aaatttatat ttcattgcaa aagataggga agattgtaga
     3661 gccttgcttg aatttgtttt atttagacaa agaaagtgta aagggagtta cctgttattc
     3721 taattagcag gtaaagttcg agaatttctc gattttttca tggcaacact tcgaacgtgt
     3781 tctgaaatat atgtttttga ggtatagggg attgatgtgt aattcttaaa gagaaaatat
     3841 tataagttgt ttgaaaaacg cttagaagtg aaaatgaaga ggctgagaga taggaataat
     3901 ttttgttttt aaaagaaaaa atgcccttgt ccacgaagtg gattgaaggg cattttttta
     3961 tggtttttcg gacacgaaaa atacggtttt cttctttctt taatacatag gtaactattt
     4021 ccgcacggaa attgatgcct atgtattctt ttatcccttg atacacaagg gatttcgaaa
     4081 aaaaagactt gctatttccg gcgggtagat ttatcatttt tagtgtcgaa ttaaaaacaa
     4141 taactaccaa ggaaggagca agtccgaaaa agggattcgt gtaccctttt ttaagcaatt
     4201 catataggac taatatatca aacatgacaa agaaaaatca agatgaaatc actggtaaat
     4261 tacaacctaa aaagaaacag aatataagaa tttatgaatt tatagagaag aaaatgagtg
     4321 agagcgggag agaattgttt aaatcgtgca gtacttttaa tgagtttgtg gctacgaaag
     4381 ataaaaagaa aaagaaacga gtaaagggta atgattgtaa aaatcgattt tgtccgattt
     4441 gtgcttggag aaaagcaggg aaggacgcgg tgaaaatcgc aacaatgatg gaagcaatca
     4501 agattgaaga aaagaaagag tttcttttct taacattgac aactccgaat attaaagctg
     4561 atacggtaaa aagcgagatt gatagattta ataaagcttt taataaatta tttaagagac
     4621 gtaatattca acgttctatt aagggttata ttagaaagct agaaatgacg tatgataaag
     4681 aacgattcat cactgaagaa atgtataaca acaaaaagag aaaagcatat tatgattctc
     4741 gtggattgaa agtagacgat cataacccga attatgatac atataatcct catttccatg
     4801 tattactttg tgttgaaaaa aattatttta aacgtaaaga gctttatatt aagcaggaag
     4861 aatggttgga aatgtggcga gaagtaacag atatgccaga gattacgcaa gtacatattc
     4921 aaaaagtaga gttaattcga gaaggaaatg cagttgcaga ggttgcgaag tattcagcaa
     4981 aagattatga aatgtcagtg agtcaagatg tgttcgacgt gttttattta gctttaaaag
     5041 gtcgacaatt aattgtgtac ggtggattgc taaaagatta tgctaaaaaa tacgaagatg
     5101 gcgaattgga caagtataag agtacagata aaaatgagta ttattataga ttaattgcaa
     5161 tgtggaataa ggatttaatg aaatttgaac aagaatatca agagttaacg gaaagtgaga
     5221 aacaagaata taacggtcat ttgattgatg aaatggaggt tgaatagttt tgtataaaaa
     5281 aggtgatatt gttaaacata cctgttggga atgttataac gaagaaatga aaatttatcg
     5341 gatttcaaaa agtaaggtca aagatggtga agatgtatat tactcatata agtgctaggt
     5401 taaatgtgaa aaatgtggtg cggttgatga tatttgaatg acaaagagag aaccagtctt
     5461 aatattaagt ttggttctct cttttttgta ttgaaagaaa accttctgag tgcagcaagt
     5521 agcattcaaa aggtttttat gatctaacag agaattaaca aaaaagagtt ttttcttaaa
     5581 tttaaatttg tgaatttgtg aatttatttt taaagtttat ttcgagatat attttgttct
     5641 gatgatttcg ttttactact gtacgagtta aaaacctata gtgtgcgcat gaattttttt
     5701 agatatatct atatcgataa aataccgcac gttttttgat gttttttgaa aatagaaatt
     5761 ctacattttt ggggttgatt ttggattttt ggtgtgggat tttatttaaa aatttttaaa
     5821 tttgaaatct tgatttttcg atttttgaaa tttttgtttt ccgttttctc tgtaattctg
     5881 tattccgaaa ttagggagtt tatatagtac ctctatgacg aactgaaaac gaaaaaactt
     5941 tgctcgcata tttaattcat agatatttca tatcgttaaa aagttgcacc gttttaaaca
     6001 agttgtgtga taaaaaaaga tgtttagatt atgtttgaat ttgttgtttc tgtgaatttt
     6061 ttatttcgtg attcctgtat atttttaatc ctttttactc ttaaaatctt tttgcttttg
     6121 tgcgcaaatg aaaaattcaa aaagccacgc gtaaccttta ttaaatatat aaatatatta
     6181 ttaaaatacc taaccctttt atgatgaaaa aatataaata gttaaagaaa aaagactgat
     6241 gaaaatatca gtcttttttc tttagtttta tttataaaga gtatctcttt atggtgtttt
     6301 ctactttttc tctccagttc tctatatcgt caattgatat tccgatcttt attaattcgt
     6361 ttggaatgaa actgttatct aaaatccata aagtgagcct ttctaatgtg attctttcgt
     6421 ttccaatcga ataaatacgt tcactttgtt tgcaggtata tggtttttta ccaacatatt
     6481 tttctacata aaagtatctt tttccgtttg ctttgtaatt tgttagaaat gccatagtaa
     6541 tgctcctttc agctgttttt atggttagtt aaagagtagt tctgtaatgt tttttcaact
     6601 ttttcatacc aatatttcac atcgtttatg gatactccga tttctagtag ttcttttggt
     6661 atacggttgt tatctaatag ccacattgcg attctttcta aagctatttt ttgattgccg
     6721 attgcgtaaa tgcatttata tttgtattgc tttgagtaat atggttgtgt tccaacgtat
     6781 tgggctacat aaaagtaaat gtttcctttt actttttttg atattatata tgccaaagaa
     6841 atgacctcca atcattaaaa atctatatta ctttgattaa tattaacttt atcaggttaa
     6901 tttttcaaca aataaagtat gaaataatgc atattttgaa cgttttaatt taaataggtt
     6961 ttaaattagt ttaaataaat aaaataaata accttatatg tatataaatt aactttaatt
     7021 taaatgaagt atgaaatagt gcataatatg attttttatt ccatacaggt ttaaaaatgt
     7081 tttataaagt gctaaagttg tgtttaaaag gttaaaaaac aactattctt tgcacaaaag
     7141 tgcccttata agaaaaaagc atgtatattt catgagaaaa aggagtataa aaaatgaaag
     7201 tagtaaataa cttccgtgtg attatggcgg taaagaatat agataatgtt gcagagttgg
     7261 ttaggatgac aggggtaagt cgtaactcta tcaataagtt gtggcgtaac gacaatgtgt
     7321 catctttacg aatagataca ttgctaccga tctgtgaaaa gttaaatgtt aaactatcgg
     7381 atttaattga atatattcca gacgatagtg agacaaaata atttaggggc tattctatct
     7441 ttagatagct tgtgtatgat gaaatcggta ttaatatttg ctagtggaaa acaggttata
     7501 ttcgcatatg gtttttggta gggttatatg cttattttaa atttcggcaa acttttatga
     7561 ctgtcttttt ctttcttttt ctgtttgtta tcacatgaat atggtaaatg aaattatagg
     7621 cttgctaggt gcgtc
//
