LOCUS       NZ_AP022980             8218 bp    DNA     circular CON 14-MAR-2024
DEFINITION  Bacillus cereus strain 30043 plasmid p30043_2, complete sequence.
ACCESSION   NZ_AP022980
VERSION     NZ_AP022980.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203339
            Sequence Read Archive: DRR206379, DRR206403
            Assembly: GCF_021655275.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8218)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 432x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655275.1-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 03:42:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,619
            CDSs (total)                      :: 5,467
            Genes (coding)                    :: 5,228
            CDSs (with protein)               :: 5,228
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 127 of 239
            Pseudo Genes (incomplete)         :: 120 of 239
            Pseudo Genes (internal stop)      :: 45 of 239
            Pseudo Genes (multiple problems)  :: 48 of 239
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022980.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8218
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="30043"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="p30043_2"
                     /geo_loc_name="Japan:Tottori"
                     /collection_date="2016"
     gene            complement(join(7754..8218,1..648))
                     /locus_tag="L6436_RS27900"
     CDS             complement(join(7754..8218,1..648))
                     /locus_tag="L6436_RS27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036645.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_236796815.1"
                     /translation="MSKITNDIDLKIDEVYEKVKDMKITFDENNTTKKQLTDSTINTYC
                     GIVKSYNNWLIQTHGISVDRAKPRHAMEYLETKIQAFEKGNASAFSMRQMAHAFHSFRV
                     ASAETGTFKHTCKVADKRDMLNTLTERGIFRKSADSTVLKANHSDYEKVQKELNVATTS
                     KHLREDIKTVHELQRYAGMRVHEAVKLKVSDIDFKSGTVYIKGKGGHERTVKIESKELL
                     EKLKNLCTGKSERSQVIIARKRDKNQLTNEEFGKRAKEEIRGAAVNAGVDRNGKRYTTH
                     SGRKAYAQAQMDKYKSYSKNQLKKEVAKRCQDKKTKQKYDAVIKNIRKNITEGSKNEGR
                     KLTNKEMCQFLVSLDLGHYRLDVVRYYADY"
                     /name="tyrosine-type recombinase/integrase"
     gene            1843..2394
                     /locus_tag="L6436_RS27905"
                     /old_locus_tag="BC30043_p21"
     CDS             1843..2394
                     /locus_tag="L6436_RS27905"
                     /old_locus_tag="BC30043_p21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_000028030.1"
                     /translation="MSISKHANKKKNILIAASEIVKEEGVVKLTLEAVAQRAGVSKGGL
                     LYHFPSKEALIKGMVEDWTNNYFECINTLVNNDDDNAIGKWNRAYLKSTFSDLENNNLN
                     SALMATMFINPDLLDEFRQRYDILHTKLITDGIDPVKITITRLSIDGLWFSEIFGMAPL
                     NEELKTQVFDELINMIQEDE"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            2394..2771
                     /gene="qacH"
                     /locus_tag="L6436_RS27910"
                     /old_locus_tag="BC30043_p22"
     ARG             2395..2768
                     /name="qacH"
                     /gene="qacH"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacH"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3101..3982
                     /locus_tag="L6436_RS27915"
                     /old_locus_tag="BC30043_p23"
     CDS             3101..3982
                     /locus_tag="L6436_RS27915"
                     /old_locus_tag="BC30043_p23"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="WP_236799909.1"
                     /translation="MNLSIQDELQLFSEELYRHLTPSLLEELAKELGFVKRKRKFSGNE
                     LATICIWVSQRTASDSLVRLCSQLHAATGTLMSPEGLNKRFDKKRLNFEIYFLALWKSK
                     ICETSAISSAALTYFQRIRILDATIFKYKTFSPCISWIGWLCTNSGYQIQLEYDLHSGQ
                     FLNFQIGPGKNNDKTFGTECLDTLRPSDLCIRDLGYFSLEDLDQMDRRGAYYISRLKLN
                     HTVLCKKSVSRILSKRNRKKQSQYIQVDLEHIMNNLKPGQTYEIKEAHWKESKTIHTCN
                     YLSFNRQTDTGT"
                     /name="IS4 family transposase"
     gene            4143..4208
                     /locus_tag="L6436_RS27920"
     CDS             4143..4208
                     /locus_tag="L6436_RS27920"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236799911.1"
                     /translation="MFKTWKSLFQIHHWQTIKKSD"
                     /name="hypothetical protein"
     gene            4181..4528
                     /locus_tag="L6436_RS27925"
                     /old_locus_tag="BC30043_p24"
     CDS             4181..4528
                     /locus_tag="L6436_RS27925"
                     /old_locus_tag="BC30043_p24"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236799908.1"
                     /translation="MANYQKERLECHVYGRLIAIFICSSTMFKMRKLLLQKNKRELSEY
                     KAIGMIQDHVSLLYQAIQRNTQNLTKILIRLFDLLQKNGRKSHRYEKKTIFDIMGVVYE
                     YNGFGKQKKAA"
                     /name="hypothetical protein"
     gene            complement(5212..5832)
                     /locus_tag="L6436_RS27930"
                     /old_locus_tag="BC30043_p25"
     CDS             complement(5212..5832)
                     /locus_tag="L6436_RS27930"
                     /old_locus_tag="BC30043_p25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000963189.1"
                     /translation="MAIDFSQADRNARKRDLEKEKFFSDEEWELANDLQSRANARGMKL
                     VPERKIKNRAKFAQMLQDNVDYLEEIDYLDDKEIIFLWKISRKVGFLSNCLVDDIHKKS
                     PVPLTQTDIATLLKRTKNNVNPIVKSLVAKGILAVSKTGVQGNNSRANVYFMNPNILYA
                     GSKDDVNLTLQAMFSKVPKALKDMPVKLFELSCHSEEEEEKTN"
                     /name="hypothetical protein"
     gene            complement(6083..6520)
                     /locus_tag="L6436_RS27935"
                     /old_locus_tag="BC30043_p26"
     CDS             complement(6083..6520)
                     /locus_tag="L6436_RS27935"
                     /old_locus_tag="BC30043_p26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000094076.1"
                     /translation="MSSFRWYEENEVIGKTIIEEDDLTLLEAGKIYIDAIFCERIRFPV
                     QKNEQPIGFNFVYEYNVKKYFGLYDRSFMKIIKQEDLEEYHMRMIDFHVHRLSLGEELD
                     DCPIIGICYGSGPLITPRLEQVYDVSKTKAYRKYRLEKLFD"
                     /name="hypothetical protein"
     gene            complement(6534..6941)
                     /locus_tag="L6436_RS27940"
                     /old_locus_tag="BC30043_p27"
     CDS             complement(6534..6941)
                     /locus_tag="L6436_RS27940"
                     /old_locus_tag="BC30043_p27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000037919.1"
                     /translation="MSKKISIEEMTQEMKEKFTISLEDAHKILLDHGVTKSTNTQVTMR
                     WIRDGKIDALLIGKGRVETRKWLINKESLMEFVAFERLTLGDFKAMKEELEMLRAFKFE
                     VEKKPKRATGTRQKKDAATNTSKEKKEVPTK"
                     /name="hypothetical protein"
     ARG             2395..2771
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="60.75"
                     /coverage="116.82"
ORIGIN
        1 aattttaacc gttctctcgt gcccgccttt gccttttata tagacagtgc cactcttaaa
       61 gtctatatca ctcactttta gttttacggc ttcgtgaaca cgcattccag cgtaccgctg
      121 taattcgtgt actgttttta tatcttctct caaatgttta cttgtcgttg ctacatttag
      181 ttctttttga actttttcat agtctgaatg atttgccttt aataccgtcg aatcagccga
      241 tttacggaag attccacgct ctgtgagtgt atttaacata tctcgtttat cagcgacctt
      301 acatgtatgt ttaaacgttc ctgtttcagc actcgcaaca cgaaaagaat gaaacgcatg
      361 cgccatttgg cgcatagaaa atgcggaagc atttcctttt tcaaaggctt gaatttttgt
      421 ttcgagatat tccattgcgt ggcgaggttt cgccctatcc acgctaattc catgcgtttg
      481 tattaaccaa ttgttatatg atttcacgat tccgcaatat gtgtttattg ttgaatcagt
      541 taattgtttt ttggttgtat tattttcatc aaaagttatt ttcatatctt taactttttc
      601 atagacttca tcaattttta aatcaatatc atttgtaatt ttgctcattt cgtcaccccc
      661 tatatacaaa atcaagagca agaacccaac ccccgtgaac cttacaatat caaggcacaa
      721 caaaaacgag gtctcatttg ggtgggttcg atctattttt tttaaagacc aaaaaaaata
      781 gaccgaacca cccaaattaa acacacaaaa gcaatcgcga aatctccctt cgggcgattt
      841 aagctcacta gagtcaaagt caaaatcaaa agaccaaaag caaaaatcaa ggtcaaaaga
      901 ttaaaagaca aaggctcaag atagctacgc catcttgttg cgattggcgt ctataaatag
      961 actccaaggc tctcccatac attttgcacc aaaatgtata gtcaaatgag cgtacaacgg
     1021 tattgcggtc tggaattttt gagtaaccca gaaattactg ttcttgcgat ttgttttgtg
     1081 gttaaaaacc tttgttacag atttacattt attcagttgt aaaaatattt aattgtaaaa
     1141 cttgttccct tcgtattttt tgatttgtca tctccttaaa tttttatttt ttataatatt
     1201 tttttccatt cttgcaagca agaattctat tttcttttaa agctatttat aaaactactt
     1261 gggaaagctt tatattgctc ctttcttaga ttattttttc ccacgtctta aaaacatttt
     1321 gctttgctat tgtaatgatc ccctttcatt tttatgacgt gaatttttta gttttgtaaa
     1381 atacacgatt acttgaatca cgtcccaaga ttcatagtat aaaatttcgt atttcatatt
     1441 tgcttaaccg caagcgttta aacaaataag taattggttt taaaattact atgaatatat
     1501 acaaatttta ccattaatca actgattttg atgaaaaaac cttatttttt tatgcttttg
     1561 aaaaggtctt acgaccttaa atgtttttct ttccggggta gagtcctgaa aatgcggatt
     1621 tacaacggta atgtaatttt cacccataaa taaagccgat tttaagaaat cgactgttga
     1681 actaaagctc cctttacttt aagtacattt cttcacaatc tttagattaa tttattggat
     1741 tatttttatg tgaaagagtg atattaaata agagctttaa ttcgattgac aaaaaaaccg
     1801 tccagacggt atattatatg tatctaagtt atggaggtta ttatgtctat ttcaaagcat
     1861 gctaataaga aaaaaaatat attgattgct gcatctgaaa ttgtaaaaga agaaggagtt
     1921 gtcaaactca cattagaggc agtcgcccaa agagctggcg tgagtaaagg tggattatta
     1981 taccattttc ctagcaagga agcattaatt aaaggaatgg tggaagattg gacaaataat
     2041 tatttcgaat gcattaatac gttagttaat aacgacgatg ataacgcaat aggaaaatgg
     2101 aatagggctt atcttaaatc tacattttcc gatttagaaa acaataatct aaattccgca
     2161 ctaatggcta ctatgtttat taatccagat ttattagatg aattccggca gagatacgat
     2221 attttgcata caaagttaat aactgatgga attgatcctg tcaaaataac aattacaaga
     2281 ttatccatag atggtctttg gttttcagaa atatttggaa tggcaccatt aaatgaagaa
     2341 ctaaaaacac aagtttttga tgaactaata aacatgattc aggaggacga ataatgtcat
     2401 atctatattt agcacttgca atagtggggg agattattgg ctcttcccta ttaaaagcat
     2461 ctgaaggctt ttcaaaactc tatcctacaa taggggtaat aatagctttc gttggttgtt
     2521 ttttcttttt atccttgtcg ctaaaaacaa tccccttaaa cactgcatat gcattatggg
     2581 caggtttagg acttgttttg actacagtaa tttcagtttt agtttggaag gaaaaactta
     2641 acatggctag cattgcagga ataacactga ttcttgtagg agttgtaata ttgaatttgt
     2701 ttggaccagg acatggagaa tctggtcatg aagcaagcga aactattaca atgaatgaga
     2761 caatggaata acacataaaa aagtccttct taataaagag aaggactttt tagcttaatt
     2821 aagggtattt tttgaagtcc ttattcaaca aacctgcgta cgttagttta agtgtatcac
     2881 ttcacaattc caatagagta gagtttattg agacataatg aaattagcgt tttaaatgac
     2941 ttctgactgt ctcataagac ttgtatcaaa tgcttaccgt tgtaaatccg catttccctg
     3001 acgctacccc atgcccatca acttaagaat tttcactacc cccaagtgac aaaaaacgtt
     3061 attctttcta aaaagctagc tagaaagggt gacatttttt atgaatcttt cgattcaaga
     3121 tgaattacaa ctattttctg aagaactgta tcgtcattta accccttctc ttttggaaga
     3181 actcgcaaaa gaattaggtt ttgtaaaaag aaaacgaaag ttttctggaa atgaattagc
     3241 taccatatgt atttgggtaa gtcaacgtac agcgagtgac tcgcttgttc gactatgcag
     3301 tcaattacat gcggccacag gcactcttat gagtccagaa ggactcaata aacgctttga
     3361 taaaaagcgg ttgaattttg aaatatattt tttggcatta tggaaaagta aaatctgtga
     3421 aacatcggcc atttcaagcg cggcgctcac gtattttcaa cgaattcgta ttttagatgc
     3481 gacgattttc aagtacaaaa catttagccc atgtatatcc tggatcgggt ggttgtgcac
     3541 aaacagcggg tatcagattc aattagaata tgacttacat agcggacagt ttctaaactt
     3601 tcagataggt ccaggaaaaa ataatgataa gacctttgga acagaatgct tagacacatt
     3661 acgtccaagc gatttatgta tccgtgattt aggttatttt tcattagaag acttagatca
     3721 aatggatcga cgaggtgctt attatatatc cagattaaaa ttaaaccata ccgtactatg
     3781 taaaaaatcc gtttccagaa tactttcgaa acggaaccgt aaaaaacaat cacagtatat
     3841 ccaagttgat ttagaacata ttatgaacaa tttaaaaccc ggtcaaacat acgaaataaa
     3901 agaagcacat tggaaagaat caaaaactat tcacacgtgt aattatctat cgtttaacag
     3961 acaaacagat acaggaacgt agaaaaaaac aaagttacac ttgaaagtaa aaagggtatt
     4021 acattttcag aaaagagtaa acgattaacg ggtatcaaca tatatgtctc aaatacgcct
     4081 gaaggagttg ttccgatgga acaaatacat gactttactc ccttcgctgg caaatagaaa
     4141 ttatttttaa aacgtggaaa tccttatttc aaattcatca ttggcaaact atcaaaaaga
     4201 gcgattagaa tgtcatgtgt atggaagact tattgccatc tttatctgtt cttctacgat
     4261 gtttaagatg cgtaaacttc ttttgcaaaa gaacaaaaga gaattaagtg aatataaagc
     4321 cattgggatg attcaagatc atgtatcgct gttatatcaa gcgatacaga gaaacaccca
     4381 aaatctaaca aagattttaa tccgtttgtt cgacctccta cagaagaatg gacggaaatc
     4441 tcatcgatat gagaagaaaa ctatctttga tattatgggt gttgtctatg agtataatgg
     4501 gtttggaaaa caaaagaaag ctgcataatt tttaaaatga aactcgttag agtttatttg
     4561 gtgtgtgtac ttttcaatac caaaagtaca ttacgtgttt tttagtttgt ttgaacgaaa
     4621 gttcattttg ttttcgttct taagttgatg ggcatgtgac gctacccctt tcctaagcaa
     4681 aaatgttttt aagtctttta ttcttttacc gtattaaatt tgatacggtt ttcatatgct
     4741 tttttgggtt gagctggctg gggtttgggg ctagccccaa gggtttttat tacacaaaat
     4801 attgatagaa cggaaatgta cgcactaagc gacggagcaa ttaaaatgga ttgttcaagt
     4861 gataatccga aaaagtgata acttttttgg tttatcgctt ggcacaagcc atgccaattg
     4921 cggagaattt tgagtcaaga taaagagtgg agattttttg cggtttttgc aaaaaatact
     4981 actcgtttct ttactcaaaa ttagcgttaa tagagagtga gatagaacaa cattgttaat
     5041 gtattaaaaa gtcaataata tcaatggttt gttgcacttt tcgtagttct tgaatttaag
     5101 aaccatagtt cttgaattta agaacaccgg attttttaca ttttttacat tattttacat
     5161 aattgaaaga taaaaaatac atgtattaaa aaatacatgt attaattatt attaatttgt
     5221 tttctcttct tcttcttctg aatgacatga tagttcgaat aattttacag gcatatcctt
     5281 taatgcttta ggaacttttg aaaacatcgc ttgtaatgtt aaattgacat catctttgct
     5341 accggcgtac aaaatgttcg gattcataaa atacacattt gctctcgaat tattaccttg
     5401 aacacctgtt tttgaaacag ccagtatgcc ttttgctact aaagatttaa caataggatt
     5461 gacattattt ttagttcttt tcaacaaagt tgcgatatct gtttgtgtta aaggaactgg
     5521 acttttttta tgaatatcat ccacaagaca atttgataaa aaaccaactt tacgactgat
     5581 tttccataaa aatataatct ctttatcatc taaataatca atctcttcga gataatcaac
     5641 gttatcctga agcatttgag caaattttgc tcgattcttg attttacgct ctggaacaag
     5701 tttcattcct cgcgcgttag ctcttgattg caaatcattt gctaattccc attcttcatc
     5761 tgaaaaaaac ttttcttttt ctaagtctct ttttctagcg tttcgatccg cttgactaaa
     5821 atcaattgcc attgattttg ctcctctcct gaagaaaatg caacaagcat tctaggagat
     5881 tcaccgttat ttttttatta tccgtaaagg gttttaccct tgatttttaa atgaaaattc
     5941 attaaaatta aaactagaaa tcaacgtttt ggtgataaat tacgatgaat cacccaaagt
     6001 cgagtgctcc aacactcggc ttttttttat gcttgtctat tcaattctag cttaaaaact
     6061 caaattttgt aagatgcttt acctaatcga ataatttttc taatcgatac tttctgtatg
     6121 cttttgtttt cgaaacatca taaacttgtt ccaacctcgg tgttatcaaa ggtccacttc
     6181 cataacaaat accaatgatt ggacaatcat ctaattcttc ccctaaagat aatctgtgca
     6241 catgaaaatc tatcattctc atgtgatatt cttctaaatc ttcttgttta attatcttca
     6301 tgaaacttct gtcatataat ccgaaatatt tttttacatt atattcataa acaaaattaa
     6361 atccaattgg ctgttcgttt ttttgaactg gaaaccttat tctctcacaa aatattgcat
     6421 caatatatat tttcccagct tctagcaaag tcaaatcatc ttcttctatt atagtttttc
     6481 caatcacttc attttcttca taccatctaa aagatgacat gcaattcacc tctttatttt
     6541 gttggcactt ctttcttttc ttttgaagta tttgttgctg catctttctt ttgacgtgta
     6601 ccagtagcac gctttggctt tttctctact tcgaatttaa aagcacgtaa catttctaat
     6661 tcttctttca ttgctttgaa atcacctaac gttaaacgct cgaatgcaac gaattccatt
     6721 agactttcct tgttaataag ccattttcga gtctccacac gaccttttcc aatcaataat
     6781 gcatcaattt tcccatcacg aatccaacgc attgtaactt gtgtattcgt acttttcgtt
     6841 acgccatgat ccaaaaggat tttatgtgca tcttccagtg aaatcgtgaa tttttccttc
     6901 atttcttgcg tcatttcttc aattgagatt ttttttgaca ttgttttttc ctcctaatta
     6961 caattaagta catgtatttt taattaaatg tatccatcta tatttttaat atacagtaaa
     7021 tgtattgaaa agtaaatata attttaaata aatttattaa aaaatatatt taatctattt
     7081 tacatttact attaattata tttactcaaa attatatata tccttcttta aatttattat
     7141 acattaaatt tatttttatg taaatccatt ttaggtcatt acttttactt ttgtgtaaat
     7201 ctatttattg ccatgacttt actattttgt acatgtattt tatatctctt aaacgttgtt
     7261 aaatcaacga ttttttgcga ttactaaaaa taaattgcgg agacaataca tgtattttta
     7321 tctatatttt tacttttgtg taaatctaat ttaggtatat aaatttacta ttgtgtaaaa
     7381 ctattaaaat tactttaaaa tacttttact caaaaataaa tttactatac aataaatgta
     7441 ttttaaaata cttttactgt atagtaaatt taataggcgt ttcatttttt tttgattttc
     7501 gaaaaaaagc gagaaatggc gaggatttgg aaaactctcg tgtatcttct tagttcatcg
     7561 acccgttttt tggcgatttc ctcgaatccg cacaaggtac tggcattgcc atgagaggct
     7621 aacacaaggc tttctcattg taaagccggg aaagatggct tgacaacgag gctctagccc
     7681 ttgtgtttct aagccagtgc gccagtacca actgtgctcc gcactcgtct tcccgatttt
     7741 ccgtcaaaac ctatcaataa tcagcataat atcgtacaac atctaaacga tagtgaccca
     7801 aatccaagct aactagaaac tggcacatct ctttatttgt aagtttacgc ccttcgttct
     7861 tcgaaccttc ggttatgttt ttacggatat tttttataac tgcatcatat ttttgctttg
     7921 ttttcttgtc ctgacaacgt tttgcaactt ctttttttaa ttgattctta ctgtaacttt
     7981 tgtatttatc catttgcgct tgtgcatatg ctttgcgacc tgaatgtgtt gtgtatcttt
     8041 ttccgttacg atccacccca gcattgacgg ctgcacctcg tatttcttct tttgcacgtt
     8101 ttccaaattc ctcatttgtt aattgatttt tatcacgttt tcgagcaata attacctggc
     8161 ttctttcact ttttccagtg cataaatttt tcaacttctc tagtaattct ttactttc
//
