LOCUS       NZ_AP022979             8319 bp    DNA     circular CON 14-MAR-2024
DEFINITION  Bacillus cereus strain 30043 plasmid p30043_1, complete sequence.
ACCESSION   NZ_AP022979
VERSION     NZ_AP022979.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203339
            Sequence Read Archive: DRR206379, DRR206403
            Assembly: GCF_021655275.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8319)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 432x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655275.1-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 03:42:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,619
            CDSs (total)                      :: 5,467
            Genes (coding)                    :: 5,228
            CDSs (with protein)               :: 5,228
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 127 of 239
            Pseudo Genes (incomplete)         :: 120 of 239
            Pseudo Genes (internal stop)      :: 45 of 239
            Pseudo Genes (multiple problems)  :: 48 of 239
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022979.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8319
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="30043"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="p30043_1"
                     /geo_loc_name="Japan:Tottori"
                     /collection_date="2016"
     gene            join(7886..8319,1..274)
                     /locus_tag="L6436_RS27830"
     CDS             join(7886..8319,1..274)
                     /locus_tag="L6436_RS27830"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788735.1"
                     /translation="MQTISKFFNVDNESKLNELTIAKGELNSKESELQSKLSRVERALE
                     LAQTDLMIDDSTANKKVVAKYEEAQEKFQSELIATQEELNKVSDEIAAIVKEELHAELV
                     DAAKQDVMLIELPEKCRKAKKVINRDLYRATSFEDEGRFSRKYLHVSATVIDDARTFAD
                     IHRFADKPNLTRIGYQRGELIRWGGDNNLFKLNDEYETIYEEEAQKIKDKTDKEYEQFM
                     KAFNKYFAVSDSH"
                     /name="hypothetical protein"
     gene            328..753
                     /locus_tag="L6436_RS27835"
                     /old_locus_tag="BC30043_p101"
     CDS             328..753
                     /locus_tag="L6436_RS27835"
                     /old_locus_tag="BC30043_p101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788712.1"
                     /translation="MSKDTLKNIFHVYCFYTVRLQGDTEPRMRRNKLNYDNPIQNYYES
                     FIDCLRGFCEVRSDVSLYSFYQFVVDAVNSLNKQERKLIYERYLYKDHYKSDRQHYLAM
                     DITPQNYKKQMDPVRRKLIKNLGIEDLQLNIPDWMKR"
                     /name="hypothetical protein"
     gene            884..1132
                     /locus_tag="L6436_RS27840"
                     /old_locus_tag="BC30043_p102"
     CDS             884..1132
                     /locus_tag="L6436_RS27840"
                     /old_locus_tag="BC30043_p102"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_236788716.1"
                     /translation="MKSLYRILELDKDEVSSSEKLILLTIAIYSDNKFIPFSITELENY
                     TNLSRATVTRLVKQLEEKGFVEVQRNGTATNNYKVTL"
                     /name="helix-turn-helix domain-containing protein"
     gene            1209..1385
                     /locus_tag="L6436_RS27845"
                     /old_locus_tag="BC30043_p103"
     CDS             1209..1385
                     /locus_tag="L6436_RS27845"
                     /old_locus_tag="BC30043_p103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176543923.1"
                     /translation="MLPQKEQLGHLAEKTELVKDLAWIAHDLLTEEDYTKENAAEALIK
                     VINRELSYVSKVR"
                     /name="hypothetical protein"
     gene            1427..1633
                     /locus_tag="L6436_RS27850"
                     /old_locus_tag="BC30043_p104"
     CDS             1427..1633
                     /locus_tag="L6436_RS27850"
                     /old_locus_tag="BC30043_p104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788718.1"
                     /translation="MANTVAKDKFEIVEKFVNACMLKGVSEDKSFKLVEELCTLIQQKR
                     HDEWFGLVDEALKEAGHEEGVTL"
                     /name="hypothetical protein"
     gene            1630..1761
                     /locus_tag="L6436_RS28100"
                     /old_locus_tag="BC30043_p105"
     CDS             1630..1761
                     /locus_tag="L6436_RS28100"
                     /old_locus_tag="BC30043_p105"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272880692.1"
                     /translation="MSEVEMVLHEFEELLHKIISAAQHGDEQAMNHLDIVKAWLKDN"
                     /name="hypothetical protein"
     gene            1838..2077
                     /locus_tag="L6436_RS27855"
                     /old_locus_tag="BC30043_p106"
     CDS             1838..2077
                     /locus_tag="L6436_RS27855"
                     /old_locus_tag="BC30043_p106"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788720.1"
                     /translation="MAEEHRYHVTFYFSNGKEFDGKVTSKYNKEDYLEGLEDLFLKEKT
                     LLIDHLGMLIQTKYITHVKVIEVGSKIGADKKDT"
                     /name="hypothetical protein"
     gene            2138..2674
                     /locus_tag="L6436_RS27860"
                     /old_locus_tag="BC30043_p107"
     CDS             2138..2674
                     /locus_tag="L6436_RS27860"
                     /old_locus_tag="BC30043_p107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3967 domain-containing protein"
                     /protein_id="WP_236788722.1"
                     /translation="MTDKTDSSQSIYISKDVAMMLKIQESTLRKYCIMLEEHGYKFHKN
                     EHGHRGFLDNDVITLRKLIEIKSHPDMTLKQACNAIMTWVKEKDMSEVDTNVITENEQH
                     DERYSELKEMIQQQNELLKKMAKKLDDQQRYIDEKLEKRDQQLMGAIREMQEEKRVLLE
                     TASASQEKKWYKFWK"
                     /name="DUF3967 domain-containing protein"
     gene            3298..3897
                     /locus_tag="L6436_RS27865"
                     /old_locus_tag="BC30043_p108"
     CDS             3298..3897
                     /locus_tag="L6436_RS27865"
                     /old_locus_tag="BC30043_p108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562260.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_236788723.1"
                     /translation="MNKRFAYIRVSDKTQNENRQLDEMLQQEIEERDIFIDKASGKDFN
                     RPKYQALKQMLRAGDILYIKSIDRFGRNKDDILKEWNEITKEIGADIVVLDMPLLDTRQ
                     YKDVLGSFVSDLVLQILSFFAEEERRKIKERQREGIDAAQRRGAKFGRPAATFPPGFEE
                     VYKRWKDGEIKAIEAMEELELKRTTFYKLVNSFENK"
                     /name="recombinase family protein"
     gene            4653..4850
                     /locus_tag="L6436_RS27870"
                     /old_locus_tag="BC30043_p109"
     CDS             4653..4850
                     /locus_tag="L6436_RS27870"
                     /old_locus_tag="BC30043_p109"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788725.1"
                     /translation="MIGKDMITLDNPKKEYFQQYAQNNKEQINANARRRRQQQKEKVEK
                     YDRLVAALRSRGISVEELLK"
                     /name="hypothetical protein"
     gene            5273..6373
                     /locus_tag="L6436_RS27875"
                     /old_locus_tag="BC30043_p110"
     CDS             5273..6373
                     /locus_tag="L6436_RS27875"
                     /old_locus_tag="BC30043_p110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205124.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_098492605.1"
                     /translation="MNHEEFINSTEIDDKFLKQIIDNREFNDVIVSYYERLHKESRDDG
                     MKKKQENVKGCNDFWIIDKYHEHRLKEFKRTNLCKDKFCNNCKKVKQASRMGKFIPLIQ
                     PYAENMYQLTLTIPNVKGDELKDTLSKMQKAFARLIEYFKGKKKINGIDFEVLQYEGAI
                     RSLEITFNGNEYHPHYHALIVLKVNKFHDCMIRKHKNVYSIDFTGKREARLFSDIEVLI
                     QKVWYLIINGKRITETAIQSLKKGYSCQLDKFSENDFVEIFKYMTKATTEDGKTLSYKQ
                     FKTLYYALFKVRQIQGYGIFYNLKDDEEINFDEVNEAYAEFIEELQTKEKPVTVSEAPE
                     QVRKESDYTVFSRKTFQKYWKQVMSE"
                     /name="protein rep"
     gene            6841..6990
                     /locus_tag="L6436_RS27880"
                     /old_locus_tag="BC30043_p111"
     CDS             6841..6990
                     /locus_tag="L6436_RS27880"
                     /old_locus_tag="BC30043_p111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788727.1"
                     /translation="MEREKCSLKEAKNAYNRKWRAANRDKVKLYNDRYWEKKAKEMEEQ
                     ACQK"
                     /name="hypothetical protein"
     gene            6978..7250
                     /locus_tag="L6436_RS27885"
                     /old_locus_tag="BC30043_p112"
     CDS             6978..7250
                     /locus_tag="L6436_RS27885"
                     /old_locus_tag="BC30043_p112"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788729.1"
                     /translation="MSEVSMETVIKGKQQSELLKHLEKVGIELLGRRDELLEQWEKEGL
                     KEDSIFEDDLKFVEELMNRNDELMFDVKVELITTMDEIHHQKMGY"
                     /name="hypothetical protein"
     gene            7255..7506
                     /locus_tag="L6436_RS27890"
                     /old_locus_tag="BC30043_p113"
     CDS             7255..7506
                     /locus_tag="L6436_RS27890"
                     /old_locus_tag="BC30043_p113"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788731.1"
                     /translation="MLGKYDGLLEKERLLKRIDELSFALEASGDPMLEAMVKNSGNTDI
                     AVAKELITKGSELGMNEHVFKKALLAKVNELREEVTKL"
                     /name="hypothetical protein"
     gene            7503..7916
                     /locus_tag="L6436_RS27895"
                     /old_locus_tag="BC30043_p114"
     CDS             7503..7916
                     /locus_tag="L6436_RS27895"
                     /old_locus_tag="BC30043_p114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788733.1"
                     /translation="MKKDKQKDLPFNHKVVLSVLPLGRSKAITTKEIMARSGIKDKRAV
                     SAILSELVTVYKQPIATTSKKEFKGVFIVQDVIDYQIGKEGLVSRKRHIDKRDSAYTEA
                     CLEIFKGTVTLDESKNKVEGSVRACKQSASSSM"
                     /name="hypothetical protein"
ORIGIN
        1 aaagtatttg catgttagtg ctacagtgat cgacgacgct cgtactttcg ccgatataca
       61 tcgttttgct gacaaaccaa atctaacacg aatagggtat caacgcggtg aattgatacg
      121 ttggggtggc gataataacc tgttcaaatt gaatgatgaa tatgaaacga tatatgaaga
      181 agaagcacag aaaatcaaag acaaaacgga taaagagtat gagcagttca tgaaggcttt
      241 caataagtat ttcgctgtgt cggattctca ttaatcaaaa tacataagtc gcctaagggc
      301 ggcttttttt tattaggagg gattttcatg agtaaagata cattaaaaaa catctttcac
      361 gtgtattgtt tttatacagt acgtttgcaa ggcgatacag aaccacgtat gagacgaaac
      421 aagctaaatt atgataatcc tatacaaaac tattatgaaa gttttataga ctgtctgaga
      481 ggattctgtg aggttaggag cgacgtttca ttgtattcgt tctatcaatt tgttgtagat
      541 gctgttaatt cgttaaataa gcaggaaaga aagctgatct atgagcggta tttatataaa
      601 gatcactaca aatcagatag gcaacattac cttgcgatgg atataacacc acagaattat
      661 aaaaaacaga tggatccggt aagaaggaag ttaattaaga acctcggaat agaagattta
      721 cagttgaata ttccagattg gatgaaacga taacgggtta aattacatgt atggttatat
      781 tatttttaaa ttttattttg attctattgc attttactat actcattgtt atactagata
      841 atgtaaacaa catgttgatt tataggaggt tactagttta attatgaaat cactatatcg
      901 tattcttgaa ttggacaaag atgaagtttc ttcttctgaa aaactaatct tgctaacgat
      961 agcgatctat tcagacaaca agtttatccc attctctatt acagaattgg agaattacac
     1021 gaatttgagt agagctacgg ttacaaggct cgtaaaacag ttagaagaaa agggattcgt
     1081 cgaggttcaa aggaatggaa cagcaacaaa caattacaaa gtaacactgt aaaaacaaag
     1141 aaagagcagg agattgcggc tcctactctt tcaaaacaca ttatccctta tggaaaggat
     1201 gtattagtat gttaccacaa aaagaacagt taggacatct tgctgagaaa actgaattag
     1261 ttaaggattt agcttggatt gcacatgatt tacttaccga ggaagattac accaaagaga
     1321 atgcagcgga agcgttaatt aaggtcatta atagggaatt atcatacgtt tccaaagtgc
     1381 gttaaacggg attatatgta aataaaacaa ttcggaggta atattcatgg caaatacagt
     1441 tgctaaagat aaatttgaga tcgtagaaaa gtttgtgaat gcttgtatgt taaaaggtgt
     1501 ttcggaagat aaatcattca aattagtaga ggaattatgt acacttattc aacaaaaacg
     1561 tcatgatgaa tggtttggat tagtagatga agcacttaaa gaagcgggac atgaagaagg
     1621 tgtaacgtta tgagtgaagt agaaatggtt ttacacgaat tcgaagaact attacataaa
     1681 atcatatcag cagcgcaaca tggcgatgaa caagcgatga atcatttaga tatagtaaaa
     1741 gcatggttaa aggacaatta aggcgtggtg atccacgtct tttttttata ctgtcttggg
     1801 gaatcctact gatatatttt gaaagtaggt ggactaaatg gctgaagaac atagatacca
     1861 tgtaactttt tatttttcta acggaaaaga atttgatggt aaggtaacaa gcaaatacaa
     1921 caaagaagat tatttagaag ggttagaaga tctattcctt aaagaaaaga cattactgat
     1981 tgatcatcta ggaatgttaa tacaaaccaa atatattaca catgtcaaag ttatcgaagt
     2041 tggatctaaa ataggagcag acaaaaaaga tacgtgaatt attaaggaaa aggatatatc
     2101 attgttataa cgaatgtttt ggtagaggtg aagcgttatg acagataaaa cagacagctc
     2161 tcaatccata tatataagca aagacgtagc gatgatgctt aaaattcaag aatcgacttt
     2221 acgtaaatat tgtatcatgc ttgaggaaca cggctacaaa tttcataaga atgagcacgg
     2281 ccatagagga ttccttgata acgatgttat aacgttaaga aaattaatcg agattaaatc
     2341 acatcctgat atgacattaa aacaggcctg taacgcaatt atgacgtggg ttaaagagaa
     2401 agatatgtca gaggttgata cgaatgttat aacggaaaat gagcaacatg acgagcgata
     2461 tagtgagctt aaagaaatga ttcagcagca gaatgaatta ttaaagaaaa tggcgaagaa
     2521 attagatgat cagcagaggt atattgatga aaagttagaa aaacgagatc agcagttaat
     2581 gggagctatc cgagaaatgc aagaagaaaa acgagttttg ttagaaacgg cttccgctag
     2641 tcaagaaaag aaatggtaca agttttggaa gtaggattcc ctaactatgg ggagtccttt
     2701 tttattttaa agtgagtttg gatcacttag aggtagatta aacagaaagc cctgcaggta
     2761 acatcctcag aaacgcacaa gaaaagtggt gcgtataact gggcactcgc ttttttctcg
     2821 tgaataagat ctcattgaat ggagtaattt tgtatactac tccgttggaa tatagaacgt
     2881 tttacggagc gttccaatat cgttaccttt gcaaaacaaa cacgaatacc acgttacttt
     2941 aatccgcaca caaggcaccc tttttatgca gtactcattg tagtattgca ccccttttaa
     3001 tgttgtactg caaaacaaac acaactcaac taataaagaa caaagcacat ataccggtaa
     3061 ttgaatggta acgggtgtta aggaatgata aggagtatta gtgcaaaatg aatgtagtag
     3121 tattttagta atttgcaaaa caaacgaact caattaatgt attcgtaaaa gtacacattt
     3181 acgaataaga aaaagaataa gaaaaagata ttgttttgtg ttcgtataag ttgtatgatt
     3241 agtttacaaa cattcgtaaa ttgaaaaaca cttttacgaa caaaggggaa gaggaaaatg
     3301 aacaaacgtt tcgcttatat ccgtgtatca gataaaactc aaaacgaaaa tagacagttg
     3361 gacgagatgt tgcagcagga gattgaagaa agagatatct tcattgacaa ggcaagcggt
     3421 aaagatttta atcgtcctaa ataccaggct ttaaaacaga tgctacgtgc cggagatata
     3481 ctgtatatca aatctattga tcgttttggt agaaataaag atgatatttt aaaagagtgg
     3541 aatgaaataa cgaaggagat cggtgcggat atcgttgtat tggacatgcc tttattagat
     3601 acaagacaat ataaagatgt attaggctcg tttgtaagtg atttggtact acagatacta
     3661 tcattctttg cagaagaaga aagacggaaa atcaaagaga gacaacgtga gggcattgat
     3721 gccgcacaaa gacgaggtgc gaagttcgga agaccggctg ctacattccc gccaggattc
     3781 gaagaagtgt acaagcgttg gaaagatgga gaaattaaag caatagaagc tatggaagaa
     3841 ttagagttaa aacggactac tttctataaa ctagtaaatt cattcgaaaa taagtaaaaa
     3901 aagtaataat aatattaaac tttttacaaa attaagtcga aaaagcaaag ttaaaaaaag
     3961 acaaacatac cgataacaag cattgatatt acacataatt ttatgccttt catgtcgaaa
     4021 agtaaaagtt aaaaaagtaa taaaaaagca aagtataaaa agtatacttt taagtttgct
     4081 tgtttctatg aaaatccgtt ggtataatag ttttaacgtg tttgagtgtt tcaaacgaaa
     4141 agaaaaaagc cgagttgggg aactcgactt aagtctttct aaaagtatgg ggatactttt
     4201 agattagatt ttgtgtctcg acaacacttt cgactaatta gcactaccaa tgcttattag
     4261 tcattgatga ttttacgaat catcttaacg attatttttt tatggattga cacctatttt
     4321 atcgaccttt gacaatcaaa taattaggta ccatttatat ctaagattga attaacaacc
     4381 ttatttaacg tatgaaaaag tactgtaggt caagttgtaa actataaaaa gtttcttctt
     4441 aggaaacctt aagagaaaaa aatatagata caatttgtat cgctttaata cgaaaaaaag
     4501 ttctcgttca gcgaacttcc tatgtgacaa gtttacaatg tatttttcgt tttgacaata
     4561 ctttaagtaa actcatagtt tttacataca ttgaaaggta cctctactat ttgaggtgcc
     4621 ttttttattt atttttgaaa ggatgataga aaatgatagg gaaagatatg atcacattag
     4681 acaacccaaa gaaagagtat tttcagcaat atgcacaaaa taataaagaa caaattaacg
     4741 ccaatgcaag aagacgaaga caacaacaaa aagaaaaggt agagaagtat gatagattgg
     4801 ttgcggcgtt acgatctaga ggaattagtg tagaagaatt attgaaataa gatggacaag
     4861 ctgtattaag gccggactaa gacaccagga gaagatttgt atacggtcta ggttattcgt
     4921 ggggcttatg ctacagctgt cattgtctag cggtatcgca gtgcgtaagc acgagaataa
     4981 ggagcagaca tacaccacaa gccccgaaca ctttcatgct ctccaaaaca acatgttttg
     5041 gggataccta gtgtaacgtg gccgagtgca acggaaggtt ttaatgtttc gtttaaaaaa
     5101 tgagtttctt ctattacttg atatattaga aataagtcgg ctatttctct agttttctag
     5161 taatgacaaa ttgtcgataa ccatagtcat atcaagggtt taagctattc gacttattgc
     5221 taataaggct taaaaagcct tccaaaaatt ccactagaaa tgaggtgaga aattgaatca
     5281 tgaggaattt ataaattcaa ctgaaataga tgataaattt ttaaagcaaa tcattgataa
     5341 tagggaattt aatgacgtta tagtctcgta ttatgaacga ctacataaag aatcacgcga
     5401 tgatggaatg aagaagaagc aggaaaacgt taaaggttgc aatgattttt ggattataga
     5461 caaatatcat gaacatagat taaaagagtt taaacgaact aatctttgca aggacaagtt
     5521 ttgtaacaac tgtaaaaagg ttaaacaagc ttcacgcatg ggtaaattca taccacttat
     5581 tcagccttac gcggaaaata tgtatcaatt aacgctaact attcctaatg ttaaaggcga
     5641 tgaattgaaa gatacactaa gcaagatgca aaaagctttt gctcgtttaa ttgagtattt
     5701 caaaggcaaa aagaaaataa acggaattga ttttgaagtt ttacaatacg aaggcgctat
     5761 acgatcttta gaaataacat ttaacggtaa tgagtatcat ccacattatc atgctcttat
     5821 tgttttaaag gttaataaat tccatgattg catgataaga aaacataaaa atgtttattc
     5881 aatagacttc actggaaaac gtgaggcgcg tttatttagt gatatagagg ttttgataca
     5941 gaaggtttgg tacttgatta taaacggtaa acggataaca gaaacagcga tacagtcact
     6001 taaaaaaggt tattcatgtc agttggacaa gtttagcgaa aacgattttg tagagatatt
     6061 taagtacatg acaaaagcaa caacggaaga cggtaaaaca ttgagttata agcaattcaa
     6121 aacgttgtat tatgcgttat ttaaagttag acaaatacaa gggtatggca tcttttataa
     6181 tttgaaggat gatgaagaaa ttaattttga tgaagttaat gaagcgtatg cggaattcat
     6241 agaggaatta cagacaaaag aaaaacctgt tacggtatca gaagcaccag aacaggtcag
     6301 aaaagaaagt gactatacag tgtttagtcg aaaaacgttc caaaaatatt ggaagcaagt
     6361 catgagcgaa tagccttact cattccggca agtttgataa aaggctatct ttaccaccat
     6421 taatcaaata acggagggtt ttctaatatc attatacata attttaattc gtatgatatt
     6481 aggaaattcc ttctatatcg ttaattagta gagactatga attatttcgt agtctttttt
     6541 tattggaaag gaatgattgt tgttatattt agaggtttat aacaggaaaa aggcgcctga
     6601 tctcgtttga atcagaacga aatcaagcgc ctttgggcga taattaacca agaacaagtt
     6661 aattaaataa atctaaaagt ttttggaagt acttatatta tatcgtatat ggagttcccg
     6721 gacagtaatt ttagcctaaa tttgcgtgaa gcaaatcatt aatcatacca cgtaaactat
     6781 atattcgtaa ataggtaaat gttaaaataa gagtaagaag aatgaaaaga aggtggaaaa
     6841 gtggaaagag aaaagtgttc tttgaaagaa gctaaaaacg cttataaccg aaaatggaga
     6901 gcagcgaatc gcgataaagt gaagctgtat aacgatagat actgggaaaa gaaagctaaa
     6961 gaaatggagg aacaagcatg tcagaagtaa gtatggaaac tgttattaaa ggaaagcagc
     7021 agagtgaact gttgaagcat ttagaaaaag taggtattga attactgggg cgtagagatg
     7081 aattgttaga gcagtgggaa aaagaaggac ttaaagaaga cagcattttt gaagatgatc
     7141 ttaaattcgt tgaagaatta atgaaccgaa atgacgaatt aatgtttgat gtaaaagtgg
     7201 aattaattac aactatggat gagattcatc atcagaaaat ggggtattga taggatgtta
     7261 ggaaagtatg atggattatt agaaaaagaa cggttactta aacgaattga tgaactgtca
     7321 ttcgcgttag aagcttcagg tgaccctatg ttggaagcga tggttaaaaa tagtggcaat
     7381 acagacatcg cggttgcaaa agagttgatt acaaaaggga gtgaattagg tatgaatgag
     7441 cacgtattta aaaaagcgtt gctagcaaaa gtaaatgaac ttcgtgaaga ggtaacgaaa
     7501 ctatgaaaaa agacaaacaa aaagacctcc cttttaatca caaagtagta ttgagcgtac
     7561 tacctttagg aagatctaaa gcaataacaa ctaaagaaat tatggcacgt agtggaataa
     7621 aagacaagcg tgcagtatct gctattttaa gtgaattagt gactgtatat aaacaaccta
     7681 ttgctacaac gtctaaaaaa gaatttaagg gcgtttttat tgtacaggat gtaatagatt
     7741 atcagattgg taaagaaggc cttgtgagca gaaaaaggca tatagataag cgtgattcag
     7801 cttatacaga agcgtgcttg gaaatattca aaggtacagt gacattagat gagtcaaaaa
     7861 acaaagtgga aggaagtgtt cgtgcatgca aacaatcagc aagttcttca atgtagataa
     7921 cgagagtaag ttaaacgaat taacaatagc aaaaggggaa ttgaattcca aagaatcaga
     7981 attacaaagt aagctatctc gagtagaaag agctctggaa ctggcacaaa ctgacttaat
     8041 gattgatgat agtacagcta ataaaaaggt tgttgcaaaa tatgaagaag ctcaagagaa
     8101 gttccaatca gagttaatag ctactcaaga agaacttaac aaggtttctg atgagattgc
     8161 agctatcgtt aaggaagaat tgcatgctga attagtagat gccgctaaac aggacgttat
     8221 gctaatcgaa ctacctgaaa agtgcagaaa agcgaaaaaa gtaattaaca gggatcttta
     8281 tagggcgacg tcctttgaag atgaaggtcg atttagtag
//
