LOCUS       NZ_AP022977             8228 bp    DNA     circular CON 14-MAR-2024
DEFINITION  Bacillus cereus strain 30040 plasmid p30040_2, complete sequence.
ACCESSION   NZ_AP022977
VERSION     NZ_AP022977.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203338
            Sequence Read Archive: DRR206378, DRR206402
            Assembly: GCF_021655255.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8228)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 400x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655255.1-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 03:42:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,608
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,259
            CDSs (with protein)               :: 5,259
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 197
            CDSs (without protein)            :: 197
            Pseudo Genes (ambiguous residues) :: 0 of 197
            Pseudo Genes (frameshifted)       :: 88 of 197
            Pseudo Genes (incomplete)         :: 119 of 197
            Pseudo Genes (internal stop)      :: 44 of 197
            Pseudo Genes (multiple problems)  :: 49 of 197
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022977.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="30040"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="p30040_2"
                     /geo_loc_name="Japan:Tottori"
                     /collection_date="2016"
     gene            complement(join(7948..8228,1..832))
                     /locus_tag="L6438_RS27850"
     CDS             complement(join(7948..8228,1..832))
                     /locus_tag="L6438_RS27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036645.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_236796815.1"
                     /translation="MSKITNDIDLKIDEVYEKVKDMKITFDENNTTKKQLTDSTINTYC
                     GIVKSYNNWLIQTHGISVDRAKPRHAMEYLETKIQAFEKGNASAFSMRQMAHAFHSFRV
                     ASAETGTFKHTCKVADKRDMLNTLTERGIFRKSADSTVLKANHSDYEKVQKELNVATTS
                     KHLREDIKTVHELQRYAGMRVHEAVKLKVSDIDFKSGTVYIKGKGGHERTVKIESKELL
                     EKLKNLCTGKSERSQVIIARKRDKNQLTNEEFGKRAKEEIRGAAVNAGVDRNGKRYTTH
                     SGRKAYAQAQMDKYKSYSKNQLKKEVAKRCQDKKTKQKYDAVIKNIRKNITEGSKNEGR
                     KLTNKEMCQFLVSLDLGHYRLDVVRYYADY"
                     /name="tyrosine-type recombinase/integrase"
     gene            2027..2578
                     /locus_tag="L6438_RS27855"
                     /old_locus_tag="BC30040_p21"
     CDS             2027..2578
                     /locus_tag="L6438_RS27855"
                     /old_locus_tag="BC30040_p21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_000028030.1"
                     /translation="MSISKHANKKKNILIAASEIVKEEGVVKLTLEAVAQRAGVSKGGL
                     LYHFPSKEALIKGMVEDWTNNYFECINTLVNNDDDNAIGKWNRAYLKSTFSDLENNNLN
                     SALMATMFINPDLLDEFRQRYDILHTKLITDGIDPVKITITRLSIDGLWFSEIFGMAPL
                     NEELKTQVFDELINMIQEDE"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            2578..2955
                     /gene="qacH"
                     /locus_tag="L6438_RS27860"
                     /old_locus_tag="BC30040_p22"
     ARG             2579..2952
                     /name="qacH"
                     /gene="qacH"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacH"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3285..4721
                     /locus_tag="L6438_RS27865"
                     /old_locus_tag="BC30040_p23"
     CDS             3285..4721
                     /locus_tag="L6438_RS27865"
                     /old_locus_tag="BC30040_p23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053955.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="WP_001053964.1"
                     /translation="MNLSIQDELQLFSEELYRHLTPSLLEELAKELGFVKRKRKFSGNE
                     LATICIWVSQRTASDSLVRLCSQLHAATGTLMSPEGLNKRFDKKAVEFLKYIFSALWKS
                     KICETSAISSAALTYFQRIRILDATIFQVPKHLAHVYPGSGGCAQTAGIKIQLEYDLHS
                     GQFLNFQIGPGKNNDKTFGTECLDTLRPSDLCIRDLGYFSLEDLDQMDQRGAYYISRLK
                     LNHTVYVKNPFPEYFRNGTVKKQSQYIQVDLEHIMNNLKPGQTYEIKEAYIGKNQKLFT
                     RVIIYRLTDKQIQERRKKQSYTESKKGITFSEKSKRLTGINIYVSNTPEGVVPMEQIHD
                     FYSLRWQIEIIFKTWKSLFQIHHWQTIKKERLECHVYGRLIAIFICSSTMFKMRKLLLQ
                     KNKRELSEYKAIGMIQDHVSLLYQAIQRNTQNLTKILIRLFDLLQKNGRKSHRYEKKTI
                     FDIMGVVYEYNGFGKQKKAA"
                     /name="IS4 family transposase"
     gene            complement(5406..6026)
                     /locus_tag="L6438_RS27870"
                     /old_locus_tag="BC30040_p24"
     CDS             complement(5406..6026)
                     /locus_tag="L6438_RS27870"
                     /old_locus_tag="BC30040_p24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000963189.1"
                     /translation="MAIDFSQADRNARKRDLEKEKFFSDEEWELANDLQSRANARGMKL
                     VPERKIKNRAKFAQMLQDNVDYLEEIDYLDDKEIIFLWKISRKVGFLSNCLVDDIHKKS
                     PVPLTQTDIATLLKRTKNNVNPIVKSLVAKGILAVSKTGVQGNNSRANVYFMNPNILYA
                     GSKDDVNLTLQAMFSKVPKALKDMPVKLFELSCHSEEEEEKTN"
                     /name="hypothetical protein"
     gene            complement(6277..6714)
                     /locus_tag="L6438_RS27875"
                     /old_locus_tag="BC30040_p25"
     CDS             complement(6277..6714)
                     /locus_tag="L6438_RS27875"
                     /old_locus_tag="BC30040_p25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000094076.1"
                     /translation="MSSFRWYEENEVIGKTIIEEDDLTLLEAGKIYIDAIFCERIRFPV
                     QKNEQPIGFNFVYEYNVKKYFGLYDRSFMKIIKQEDLEEYHMRMIDFHVHRLSLGEELD
                     DCPIIGICYGSGPLITPRLEQVYDVSKTKAYRKYRLEKLFD"
                     /name="hypothetical protein"
     gene            complement(6728..7135)
                     /locus_tag="L6438_RS27880"
                     /old_locus_tag="BC30040_p26"
     CDS             complement(6728..7135)
                     /locus_tag="L6438_RS27880"
                     /old_locus_tag="BC30040_p26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000037919.1"
                     /translation="MSKKISIEEMTQEMKEKFTISLEDAHKILLDHGVTKSTNTQVTMR
                     WIRDGKIDALLIGKGRVETRKWLINKESLMEFVAFERLTLGDFKAMKEELEMLRAFKFE
                     VEKKPKRATGTRQKKDAATNTSKEKKEVPTK"
                     /name="hypothetical protein"
     ARG             2579..2955
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="60.75"
                     /coverage="116.82"
ORIGIN
        1 atctttttcc gttacgatcc accccagcat tgacggctgc acctcgtatt tcttcttttg
       61 cacgttttcc aaattcctca tttgttaatt gatttttatc acgttttcga gcaataatta
      121 cctggcttct ttcacttttt ccagtgcata aatttttcaa cttctctagt aattctttac
      181 tttcaatttt aaccgttctc tcgtgcccgc ctttgccttt tatatagaca gtgccactct
      241 taaagtctat atcactcact tttagtttta cggcttcgtg aacacgcatt ccagcgtacc
      301 gctgtaattc gtgtactgtt tttatatctt ctctcaaatg tttacttgtc gttgctacat
      361 ttagttcttt ttgaactttt tcatagtctg aatgatttgc ctttaatacc gtcgaatcag
      421 ccgatttacg gaagattcca cgctctgtga gtgtatttaa catatctcgt ttatcagcga
      481 ccttacatgt atgtttaaac gttcctgttt cagcactcgc aacacgaaaa gaatgaaacg
      541 catgcgccat ttggcgcata gaaaatgcgg aagcatttcc tttttcaaag gcttgaattt
      601 ttgtttcgag atattccatt gcgtggcgag gtttcgccct atccacgcta attccatgcg
      661 tttgtattaa ccaattgtta tatgatttca cgattccgca atatgtgttt attgttgaat
      721 cagttaattg ttttttggtt gtattatttt catcaaaagt tattttcata tctttaactt
      781 tttcatagac ttcatcaatt tttaaatcaa tatcatttgt aattttgctc atttcgtcac
      841 cccctatata caaaatcaag agcaagaacc caacccccgt gaaccttaca atatcaaggc
      901 acaacaaaaa cgaggtctca tttgggtggg ttcgatctat tttttttaaa gaccaaaaaa
      961 aatagaccga accacccaaa ttaaacacac aaaagcaatc gcgaaatctc ccttcgggcg
     1021 atttaagctc actagagtca aagtcaaaat caaaagacca aaagcaaaaa tcaaggtcaa
     1081 aagattaaaa gacaaaggct caagatagct acgccatctt gttgcgattg gcgtctataa
     1141 atagactcca aggctctccc atacattttg caccaaaatg tatagtcaaa tgagcgtaca
     1201 acggtattgc ggtctggaat ttttgagtaa cccagaaatt actgttcttg cgatttgttt
     1261 tgtggttaaa aacctttgtt acagatttac atttattcag ttgtaaaaat atttaattgt
     1321 aaaacttgtt cccttcgtat tttttgattt gtcatctcct taaattttta ttttttataa
     1381 tatttttttc cattcttgca agcaagaatt ctattttctt ttaaagctat ttataaaact
     1441 acttgggaaa gctttatatt gctcctttct tagattattt tttcccacgt cttaaaaaca
     1501 ttttgctttg ctattgtaat gatccccttt catttttatg acgtgaattt tttagttttg
     1561 taaaatacac gattacttga atcacgtccc aagattcata gtataaaatt tcgtatttca
     1621 tatttgctta accgcaagcg tttaaacaaa taagtaattg gttttaaaat tactatgaat
     1681 atatacaaat tttaccatta atcaactgat tttgatgaaa aaaccttatt tttttatgct
     1741 tttgaaaagg tcttacgacc ttaaatgttt ttctttccgg ggtagagtcc tgaaaatgcg
     1801 gatttacaac ggtaatgtaa ttttcaccca taaataaagc cgattttaag aaatcgactg
     1861 ttgaactaaa gctcccttta ctttaagtac atttcttcac aatctttaga ttaatttatt
     1921 ggattatttt tatgtgaaag agtgatatta aataagagct ttaattcgat tgacaaaaaa
     1981 accgtccaga cggtatatta tatgtatcta agttatggag gttattatgt ctatttcaaa
     2041 gcatgctaat aagaaaaaaa atatattgat tgctgcatct gaaattgtaa aagaagaagg
     2101 agttgtcaaa ctcacattag aggcagtcgc ccaaagagct ggcgtgagta aaggtggatt
     2161 attataccat tttcctagca aggaagcatt aattaaagga atggtggaag attggacaaa
     2221 taattatttc gaatgcatta atacgttagt taataacgac gatgataacg caataggaaa
     2281 atggaatagg gcttatctta aatctacatt ttccgattta gaaaacaata atctaaattc
     2341 cgcactaatg gctactatgt ttattaatcc agatttatta gatgaattcc ggcagagata
     2401 cgatattttg catacaaagt taataactga tggaattgat cctgtcaaaa taacaattac
     2461 aagattatcc atagatggtc tttggttttc agaaatattt ggaatggcac cattaaatga
     2521 agaactaaaa acacaagttt ttgatgaact aataaacatg attcaggagg acgaataatg
     2581 tcatatctat atttagcact tgcaatagtg ggggagatta ttggctcttc cctattaaaa
     2641 gcatctgaag gcttttcaaa actctatcct acaatagggg taataatagc tttcgttggt
     2701 tgttttttct ttttatcctt gtcgctaaaa acaatcccct taaacactgc atatgcatta
     2761 tgggcaggtt taggacttgt tttgactaca gtaatttcag ttttagtttg gaaggaaaaa
     2821 cttaacatgg ctagcattgc aggaataaca ctgattcttg taggagttgt aatattgaat
     2881 ttgtttggac caggacatgg agaatctggt catgaagcaa gcgaaactat tacaatgaat
     2941 gagacaatgg aataacacat aaaaaagtcc ttcttaataa agagaaggac tttttagctt
     3001 aattaagggt attttttgaa gtccttattc aacaaacctg cgtacgttag tttaagtgta
     3061 tcacttcaca attccaatag agtagagttt attgagacat aatgaaatta gcgttttaaa
     3121 tgacttctga ctgtctcata agacttgtat caaatgctta ccgttgtaaa tccgcatttc
     3181 cctgacgcta ccccatgccc atcaacttaa gaattttcac tacccccaag tgacaaaaaa
     3241 cgttattctt tctaaaaagc tagctagaaa gggtgacatt ttttatgaat ctttcgattc
     3301 aagatgaatt acaactattt tctgaagaac tgtatcgtca tttaacccct tctcttttgg
     3361 aagaactcgc aaaagaatta ggttttgtaa aaagaaaacg aaagttttct ggaaatgaat
     3421 tagctaccat atgtatttgg gtaagtcaac gtacagcgag tgactcgctt gttcgactat
     3481 gcagtcaatt acatgcggcc acaggcactc ttatgagtcc agaaggactc aataaacgct
     3541 ttgataaaaa agcggttgaa tttttgaaat atattttttc ggcattatgg aaaagtaaaa
     3601 tctgtgaaac atcggccatt tcaagcgcgg cgctcacgta ttttcaacga attcgtattt
     3661 tagatgcgac gattttccaa gtaccaaaac atttagccca tgtatatcct ggatcaggtg
     3721 gttgtgcaca aacagcgggt atcaagattc aattagaata tgacttacat agcggacagt
     3781 ttctaaactt tcagataggt ccaggaaaaa ataatgataa gacctttgga acagaatgct
     3841 tagacacatt acgtccaagc gatttatgta tccgtgattt aggttatttt tcattagaag
     3901 acttagatca aatggatcaa cgaggtgctt attatatatc cagattaaaa ttaaaccata
     3961 ccgtgtatgt aaaaaatccg tttccagaat actttcgaaa cggaaccgta aaaaagcaat
     4021 cacagtatat ccaagttgat ttagaacata ttatgaacaa tttaaaaccc ggtcaaacat
     4081 acgaaataaa agaagcttac attggaaaga atcaaaaact attcacacgt gtaattatct
     4141 atcgtttaac agacaaacag atacaggaac gtagaaaaaa acaaagttac actgaaagta
     4201 aaaagggtat tacattttca gaaaagagta aacgattaac gggtatcaac atatatgtct
     4261 caaatacgcc tgaaggagtt gttccgatgg aacaaataca tgacttttac tcccttcgct
     4321 ggcaaataga aattattttt aaaacgtgga aatccttatt tcaaattcat cattggcaaa
     4381 ctatcaaaaa agagcgatta gaatgtcatg tgtatggaag acttattgcc atctttatct
     4441 gttcttctac gatgtttaag atgcgtaaac ttcttttgca aaagaacaaa agagaattaa
     4501 gtgaatataa agccattggg atgattcaag atcatgtatc gctgttatat caagcgatac
     4561 agagaaacac ccaaaatcta acaaagattt taatccgttt gttcgacctc ctacagaaga
     4621 atggacggaa atctcatcga tatgagaaga aaactatctt tgatattatg ggtgttgtct
     4681 atgagtataa tgggtttgga aaacaaaaga aagctgcata atttttaaaa tgaaactcgt
     4741 tagagtttat ttggtgtgtg tacttttcaa taccaaaagt acattacgtg ttttttagtt
     4801 tgtttgaacg aaagttcatt ttgttttcgt tcttaagttg atgggcatgt gacgctaccc
     4861 ctttcctaag caaaaatgtt tttaagtctt ttattctttt accgtattaa atttgatacg
     4921 gttttcatat gcttttttgg gttgagctgg ctggggtttg gggctagccc caagggtttt
     4981 tattacacaa aatattgata gaacggaaat gtacgcacta agcgacggag caattaaaat
     5041 ggattgttca agtgataatc cgaaaaagtg ataacttttt tggtttatcg cttggcacaa
     5101 gccatgccaa ttgcggagaa ttttgagtca agataaagag tggagatttt ttgcggtttt
     5161 tgcaaaaaat actactcgtt tctttactca aaattagcgt taatagagag tgagatagaa
     5221 caacattgtt aatgtattaa aaagtcaata atatcaatgg tttgttgcac ttttcgtagt
     5281 tcttgaattt aagaaccata gttcttgaat ttaagaacac cggatttttt acatttttta
     5341 cattatttta cataattgaa agataaaaaa atacatgtat taaaaaatac atgtattaat
     5401 tattattaat ttgttttctc ttcttcttct tctgaatgac atgatagttc gaataatttt
     5461 acaggcatat cctttaatgc tttaggaact tttgaaaaca tcgcttgtaa tgttaaattg
     5521 acatcatctt tgctaccggc gtacaaaatg ttcggattca taaaatacac atttgctctc
     5581 gaattattac cttgaacacc tgtttttgaa acagccagta tgccttttgc tactaaagat
     5641 ttaacaatag gattgacatt atttttagtt cttttcaaca aagttgcgat atctgtttgt
     5701 gttaaaggaa ctggactttt tttatgaata tcatccacaa gacaatttga taaaaaacca
     5761 actttacgac tgattttcca taaaaatata atctctttat catctaaata atcaatctct
     5821 tcgagataat caacgttatc ctgaagcatt tgagcaaatt ttgctcgatt cttgatttta
     5881 cgctctggaa caagtttcat tcctcgcgcg ttagctcttg attgcaaatc atttgctaat
     5941 tcccattctt catctgaaaa aaacttttct ttttctaagt ctctttttct agcgtttcga
     6001 tccgcttgac taaaatcaat tgccattgat tttgctcctc tcctgaagaa aatgcaacaa
     6061 gcattctagg agattcaccg ttattttttt attatccgta aagggtttta cccttgattt
     6121 ttaaatgaaa attcattaaa attaaaacta gaaatcaacg ttttggtgat aaattacgat
     6181 gaatcaccca aagtcgagtg ctccaacact cggctttttt ttatgcttgt ctattcaatt
     6241 ctagcttaaa aactcaaatt ttgtaagatg ctttacctaa tcgaataatt tttctaatcg
     6301 atactttctg tatgcttttg ttttcgaaac atcataaact tgttccaacc tcggtgttat
     6361 caaaggtcca cttccataac aaataccaat gattggacaa tcatctaatt cttcccctaa
     6421 agataatctg tgcacatgaa aatctatcat tctcatgtga tattcttcta aatcttcttg
     6481 tttaattatc ttcatgaaac ttctgtcata taatccgaaa tattttttta cattatattc
     6541 ataaacaaaa ttaaatccaa ttggctgttc gtttttttga actggaaacc ttattctctc
     6601 acaaaatatt gcatcaatat atattttccc agcttctagc aaagtcaaat catcttcttc
     6661 tattatagtt tttccaatca cttcattttc ttcataccat ctaaaagatg acatgcaatt
     6721 cacctcttta ttttgttggc acttctttct tttcttttga agtatttgtt gctgcatctt
     6781 tcttttgacg tgtaccagta gcacgctttg gctttttctc tacttcgaat ttaaaagcac
     6841 gtaacatttc taattcttct ttcattgctt tgaaatcacc taacgttaaa cgctcgaatg
     6901 caacgaattc cattagactt tccttgttaa taagccattt tcgagtctcc acacgacctt
     6961 ttccaatcaa taatgcatca attttcccat cacgaatcca acgcattgta acttgtgtat
     7021 tcgtactttt cgttacgcca tgatccaaaa ggattttatg tgcatcttcc agtgaaatcg
     7081 tgaatttttc cttcatttct tgcgtcattt cttcaattga gatttttttt gacattgttt
     7141 tttcctccta attacaatta agtacatgta tttttaatta aatgtatcca tctatatttt
     7201 taatatacag taaatgtatt gaaaagtaaa tataatttta aataaattta ttaaaaaata
     7261 tatttaatct attttacatt tactattaat tatatttact caaaattata tatatccttc
     7321 tttaaattta ttatacatta aatttatttt tatgtaaatc cattttaggt cattactttt
     7381 acttttgtgt aaatctattt attgccatga ctttactatt ttgtacatgt attttatatc
     7441 tcttaaacgt tgttaaatca acgatttttt gcgattacta aaaataaatt gcggagacaa
     7501 tacatgtatt tttatctata tttttacttt tgtgtaaatc taatttaggt atataaattt
     7561 actattgtgt aaaactatta aaattacttt aaaatacttt tactcaaaaa taaatttact
     7621 atacaataaa tgtattttaa aatactttta ctgtatagta aatttaatag gcgtttcatt
     7681 tttttttgat tttcgaaaaa aagcgagaaa tggcgaggat ttggaaaact ctcgtgtatc
     7741 ttcttagttc atcgacccgt tttttggcga tttcctcgaa tccgcacaag gtactggcat
     7801 tgccatgaga ggctaacaca aggctttctc attgtaaagc cgggaaagat ggcttgacaa
     7861 cgaggctcta gcccttgtgt ttctaagcca gtgcgccagt accaactgtg ctccgcactc
     7921 gtcttcccga ttttccgtca aaacctatca ataatcagca taatatcgta caacatctaa
     7981 acgatagtga cccaaatcca agctaactag aaactggcac atctctttat ttgtaagttt
     8041 acgcccttcg ttcttcgaac cttcggttat gtttttacgg atatttttta taactgcatc
     8101 atatttttgc tttgttttct tgtcctgaca acgttttgca acttcttttt ttaattgatt
     8161 cttactgtaa cttttgtatt tatccatttg cgcttgtgca tatgctttgc gacctgaatg
     8221 tgttgtgt
//
