LOCUS       NZ_AP022976             8319 bp    DNA     circular CON 14-MAR-2024
DEFINITION  Bacillus cereus strain 30040 plasmid p30040_1, complete sequence.
ACCESSION   NZ_AP022976
VERSION     NZ_AP022976.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203338
            Sequence Read Archive: DRR206378, DRR206402
            Assembly: GCF_021655255.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8319)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 400x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655255.1-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 03:42:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,608
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,259
            CDSs (with protein)               :: 5,259
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 197
            CDSs (without protein)            :: 197
            Pseudo Genes (ambiguous residues) :: 0 of 197
            Pseudo Genes (frameshifted)       :: 88 of 197
            Pseudo Genes (incomplete)         :: 119 of 197
            Pseudo Genes (internal stop)      :: 44 of 197
            Pseudo Genes (multiple problems)  :: 49 of 197
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022976.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8319
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="30040"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="p30040_1"
                     /geo_loc_name="Japan:Tottori"
                     /collection_date="2016"
     gene            complement(join(7997..8319,1..91))
                     /locus_tag="L6438_RS27785"
     CDS             complement(join(7997..8319,1..91))
                     /locus_tag="L6438_RS27785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788733.1"
                     /translation="MKKDKQKDLPFNHKVVLSVLPLGRSKAITTKEIMARSGIKDKRAV
                     SAILSELVTVYKQPIATTSKKEFKGVFIVQDVIDYQIGKEGLVSRKRHIDKRDSAYTEA
                     CLEIFKGTVTLDESKNKVEGSVRACKQSASSSM"
                     /name="hypothetical protein"
     gene            complement(88..339)
                     /locus_tag="L6438_RS27790"
                     /old_locus_tag="BC30040_p101"
     CDS             complement(88..339)
                     /locus_tag="L6438_RS27790"
                     /old_locus_tag="BC30040_p101"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788731.1"
                     /translation="MLGKYDGLLEKERLLKRIDELSFALEASGDPMLEAMVKNSGNTDI
                     AVAKELITKGSELGMNEHVFKKALLAKVNELREEVTKL"
                     /name="hypothetical protein"
     gene            complement(344..616)
                     /locus_tag="L6438_RS27795"
                     /old_locus_tag="BC30040_p102"
     CDS             complement(344..616)
                     /locus_tag="L6438_RS27795"
                     /old_locus_tag="BC30040_p102"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788729.1"
                     /translation="MSEVSMETVIKGKQQSELLKHLEKVGIELLGRRDELLEQWEKEGL
                     KEDSIFEDDLKFVEELMNRNDELMFDVKVELITTMDEIHHQKMGY"
                     /name="hypothetical protein"
     gene            complement(604..732)
                     /locus_tag="L6438_RS28035"
                     /old_locus_tag="BC30040_p103"
     CDS             complement(604..732)
                     /locus_tag="L6438_RS28035"
                     /old_locus_tag="BC30040_p103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272880717.1"
                     /translation="MKEAKNAYNRKWRAANRDKVKLYNDRYWEKKAKEMEEQACQK"
                     /name="hypothetical protein"
     gene            complement(1221..2321)
                     /locus_tag="L6438_RS27800"
                     /old_locus_tag="BC30040_p104"
     CDS             complement(1221..2321)
                     /locus_tag="L6438_RS27800"
                     /old_locus_tag="BC30040_p104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205124.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_098492605.1"
                     /translation="MNHEEFINSTEIDDKFLKQIIDNREFNDVIVSYYERLHKESRDDG
                     MKKKQENVKGCNDFWIIDKYHEHRLKEFKRTNLCKDKFCNNCKKVKQASRMGKFIPLIQ
                     PYAENMYQLTLTIPNVKGDELKDTLSKMQKAFARLIEYFKGKKKINGIDFEVLQYEGAI
                     RSLEITFNGNEYHPHYHALIVLKVNKFHDCMIRKHKNVYSIDFTGKREARLFSDIEVLI
                     QKVWYLIINGKRITETAIQSLKKGYSCQLDKFSENDFVEIFKYMTKATTEDGKTLSYKQ
                     FKTLYYALFKVRQIQGYGIFYNLKDDEEINFDEVNEAYAEFIEELQTKEKPVTVSEAPE
                     QVRKESDYTVFSRKTFQKYWKQVMSE"
                     /name="protein rep"
     gene            complement(2744..2941)
                     /locus_tag="L6438_RS27805"
                     /old_locus_tag="BC30040_p105"
     CDS             complement(2744..2941)
                     /locus_tag="L6438_RS27805"
                     /old_locus_tag="BC30040_p105"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788725.1"
                     /translation="MIGKDMITLDNPKKEYFQQYAQNNKEQINANARRRRQQQKEKVEK
                     YDRLVAALRSRGISVEELLK"
                     /name="hypothetical protein"
     gene            complement(3697..4296)
                     /locus_tag="L6438_RS27810"
                     /old_locus_tag="BC30040_p106"
     CDS             complement(3697..4296)
                     /locus_tag="L6438_RS27810"
                     /old_locus_tag="BC30040_p106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562260.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_236788723.1"
                     /translation="MNKRFAYIRVSDKTQNENRQLDEMLQQEIEERDIFIDKASGKDFN
                     RPKYQALKQMLRAGDILYIKSIDRFGRNKDDILKEWNEITKEIGADIVVLDMPLLDTRQ
                     YKDVLGSFVSDLVLQILSFFAEEERRKIKERQREGIDAAQRRGAKFGRPAATFPPGFEE
                     VYKRWKDGEIKAIEAMEELELKRTTFYKLVNSFENK"
                     /name="recombinase family protein"
     gene            complement(4920..5456)
                     /locus_tag="L6438_RS27815"
                     /old_locus_tag="BC30040_p107"
     CDS             complement(4920..5456)
                     /locus_tag="L6438_RS27815"
                     /old_locus_tag="BC30040_p107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3967 domain-containing protein"
                     /protein_id="WP_236788722.1"
                     /translation="MTDKTDSSQSIYISKDVAMMLKIQESTLRKYCIMLEEHGYKFHKN
                     EHGHRGFLDNDVITLRKLIEIKSHPDMTLKQACNAIMTWVKEKDMSEVDTNVITENEQH
                     DERYSELKEMIQQQNELLKKMAKKLDDQQRYIDEKLEKRDQQLMGAIREMQEEKRVLLE
                     TASASQEKKWYKFWK"
                     /name="DUF3967 domain-containing protein"
     gene            complement(5517..5756)
                     /locus_tag="L6438_RS27820"
                     /old_locus_tag="BC30040_p108"
     CDS             complement(5517..5756)
                     /locus_tag="L6438_RS27820"
                     /old_locus_tag="BC30040_p108"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788720.1"
                     /translation="MAEEHRYHVTFYFSNGKEFDGKVTSKYNKEDYLEGLEDLFLKEKT
                     LLIDHLGMLIQTKYITHVKVIEVGSKIGADKKDT"
                     /name="hypothetical protein"
     gene            complement(5833..5964)
                     /locus_tag="L6438_RS28040"
                     /old_locus_tag="BC30040_p109"
     CDS             complement(5833..5964)
                     /locus_tag="L6438_RS28040"
                     /old_locus_tag="BC30040_p109"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272880692.1"
                     /translation="MSEVEMVLHEFEELLHKIISAAQHGDEQAMNHLDIVKAWLKDN"
                     /name="hypothetical protein"
     gene            complement(5961..6167)
                     /locus_tag="L6438_RS27825"
                     /old_locus_tag="BC30040_p110"
     CDS             complement(5961..6167)
                     /locus_tag="L6438_RS27825"
                     /old_locus_tag="BC30040_p110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788718.1"
                     /translation="MANTVAKDKFEIVEKFVNACMLKGVSEDKSFKLVEELCTLIQQKR
                     HDEWFGLVDEALKEAGHEEGVTL"
                     /name="hypothetical protein"
     gene            complement(6209..6385)
                     /locus_tag="L6438_RS27830"
                     /old_locus_tag="BC30040_p111"
     CDS             complement(6209..6385)
                     /locus_tag="L6438_RS27830"
                     /old_locus_tag="BC30040_p111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176543923.1"
                     /translation="MLPQKEQLGHLAEKTELVKDLAWIAHDLLTEEDYTKENAAEALIK
                     VINRELSYVSKVR"
                     /name="hypothetical protein"
     gene            complement(6462..6710)
                     /locus_tag="L6438_RS27835"
                     /old_locus_tag="BC30040_p112"
     CDS             complement(6462..6710)
                     /locus_tag="L6438_RS27835"
                     /old_locus_tag="BC30040_p112"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_236788716.1"
                     /translation="MKSLYRILELDKDEVSSSEKLILLTIAIYSDNKFIPFSITELENY
                     TNLSRATVTRLVKQLEEKGFVEVQRNGTATNNYKVTL"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(6841..7266)
                     /locus_tag="L6438_RS27840"
                     /old_locus_tag="BC30040_p113"
     CDS             complement(6841..7266)
                     /locus_tag="L6438_RS27840"
                     /old_locus_tag="BC30040_p113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788712.1"
                     /translation="MSKDTLKNIFHVYCFYTVRLQGDTEPRMRRNKLNYDNPIQNYYES
                     FIDCLRGFCEVRSDVSLYSFYQFVVDAVNSLNKQERKLIYERYLYKDHYKSDRQHYLAM
                     DITPQNYKKQMDPVRRKLIKNLGIEDLQLNIPDWMKR"
                     /name="hypothetical protein"
     gene            complement(7320..8027)
                     /locus_tag="L6438_RS27845"
                     /old_locus_tag="BC30040_p114"
     CDS             complement(7320..8027)
                     /locus_tag="L6438_RS27845"
                     /old_locus_tag="BC30040_p114"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788735.1"
                     /translation="MQTISKFFNVDNESKLNELTIAKGELNSKESELQSKLSRVERALE
                     LAQTDLMIDDSTANKKVVAKYEEAQEKFQSELIATQEELNKVSDEIAAIVKEELHAELV
                     DAAKQDVMLIELPEKCRKAKKVINRDLYRATSFEDEGRFSRKYLHVSATVIDDARTFAD
                     IHRFADKPNLTRIGYQRGELIRWGGDNNLFKLNDEYETIYEEEAQKIKDKTDKEYEQFM
                     KAFNKYFAVSDSH"
                     /name="hypothetical protein"
ORIGIN
        1 tagttgttat tgctttagat cttcctaaag gtagtacgct caatactact ttgtgattaa
       61 aagggaggtc tttttgtttg tcttttttca tagtttcgtt acctcttcac gaagttcatt
      121 tacttttgct agcaacgctt ttttaaatac gtgctcattc atacctaatt cactcccttt
      181 tgtaatcaac tcttttgcaa ccgcgatgtc tgtattgcca ctatttttaa ccatcgcttc
      241 caacataggg tcacctgaag cttctaacgc gaatgacagt tcatcaattc gtttaagtaa
      301 ccgttctttt tctaataatc catcatactt tcctaacatc ctatcaatac cccattttct
      361 gatgatgaat ctcatccata gttgtaatta attccacttt tacatcaaac attaattcgt
      421 catttcggtt cattaattct tcaacgaatt taagatcatc ttcaaaaatg ctgtcttctt
      481 taagtccttc tttttcccac tgctctaaca attcatctct acgccccagt aattcaatac
      541 ctactttttc taaatgcttc aacagttcac tctgctgctt tcctttaata acagtttcca
      601 tacttacttc tgacatgctt gttcctccat ttctttagct ttcttttccc agtatctatc
      661 gttatacagc ttcactttat cgcgattcgc tgctctccat tttcggttat aagcgttttt
      721 agcttctttc aaagaacact tttctctttc cacttttcca ccttcttttc attcttctta
      781 ctcttatttt aacatttacc tatttacgaa tatatagttt acgtggtatg attaatgatt
      841 tgcttcacgc aaatttaggc taaaattact gtccgggaac tccatatacg atataatata
      901 agtacttcca aaaactttta gatttattta attaacttgt tcttggttaa ttatcgccca
      961 aaggcgcttg atttcgttct gattcaaacg agatcaggcg cctttttcct gttataaacc
     1021 tctaaatata acaacaatca ttcctttcca ataaaaaaag actacgaaat aattcatagt
     1081 ctctactaat taacgatata gaaggaattt cctaatatca tacgaattaa aattatgtat
     1141 aatgatatta gaaaaccctc cgttatttga ttaatggtgg taaagatagc cttttatcaa
     1201 acttgccgga atgagtaagg ctattcgctc atgacttgct tccaatattt ttggaacgtt
     1261 tttcgactaa acactgtata gtcactttct tttctgacct gttctggtgc ttctgatacc
     1321 gtaacaggtt tttcttttgt ctgtaattcc tctatgaatt ccgcatacgc ttcattaact
     1381 tcatcaaaat taatttcttc atcatccttc aaattataaa agatgccata cccttgtatt
     1441 tgtctaactt taaataacgc ataatacaac gttttgaatt gcttataact caatgtttta
     1501 ccgtcttccg ttgttgcttt tgtcatgtac ttaaatatct ctacaaaatc gttttcgcta
     1561 aacttgtcca actgacatga ataacctttt ttaagtgact gtatcgctgt ttctgttatc
     1621 cgtttaccgt ttataatcaa gtaccaaacc ttctgtatca aaacctctat atcactaaat
     1681 aaacgcgcct cacgttttcc agtgaagtct attgaataaa catttttatg ttttcttatc
     1741 atgcaatcat ggaatttatt aacctttaaa acaataagag catgataatg tggatgatac
     1801 tcattaccgt taaatgttat ttctaaagat cgtatagcgc cttcgtattg taaaacttca
     1861 aaatcaattc cgtttatttt ctttttgcct ttgaaatact caattaaacg agcaaaagct
     1921 ttttgcatct tgcttagtgt atctttcaat tcatcgcctt taacattagg aatagttagc
     1981 gttaattgat acatattttc cgcgtaaggc tgaataagtg gtatgaattt acccatgcgt
     2041 gaagcttgtt taaccttttt acagttgtta caaaacttgt ccttgcaaag attagttcgt
     2101 ttaaactctt ttaatctatg ttcatgatat ttgtctataa tccaaaaatc attgcaacct
     2161 ttaacgtttt cctgcttctt cttcattcca tcatcgcgtg attctttatg tagtcgttca
     2221 taatacgaga ctataacgtc attaaattcc ctattatcaa tgatttgctt taaaaattta
     2281 tcatctattt cagttgaatt tataaattcc tcatgattca atttctcacc tcatttctag
     2341 tggaattttt ggaaggcttt ttaagcctta ttagcaataa gtcgaatagc ttaaaccctt
     2401 gatatgacta tggttatcga caatttgtca ttactagaaa actagagaaa tagccgactt
     2461 atttctaata tatcaagtaa tagaagaaac tcatttttta aacgaaacat taaaaccttc
     2521 cgttgcactc ggccacgtta cactaggtat ccccaaaaca tgttgttttg gagagcatga
     2581 aagtgttcgg ggcttgtggt gtatgtctgc tccttattct cgtgcttacg cactgcgata
     2641 ccgctagaca atgacagctg tagcataagc cccacgaata acctagaccg tatacaaatc
     2701 ttctcctggt gtcttagtcc ggccttaata cagcttgtcc atcttatttc aataattctt
     2761 ctacactaat tcctctagat cgtaacgccg caaccaatct atcatacttc tctacctttt
     2821 ctttttgttg ttgtcttcgt cttcttgcat tggcgttaat ttgttcttta ttattttgtg
     2881 catattgctg aaaatactct ttctttgggt tgtctaatgt gatcatatct ttccctatca
     2941 ttttctatca tcctttcaaa aataaataaa aaaggcacct caaatagtag aggtaccttt
     3001 caatgtatgt aaaaactatg agtttactta aagtattgtc aaaacgaaaa atacattgta
     3061 aacttgtcac ataggaagtt cgctgaacga gaactttttt tcgtattaaa gcgatacaaa
     3121 ttgtatctat atttttttct cttaaggttt cctaagaaga aactttttat agtttacaac
     3181 ttgacctaca gtactttttc atacgttaaa taaggttgtt aattcaatct tagatataaa
     3241 tggtacctaa ttatttgatt gtcaaaggtc gataaaatag gtgtcaatcc ataaaaaaat
     3301 aatcgttaag atgattcgta aaatcatcaa tgactaataa gcattggtag tgctaattag
     3361 tcgaaagtgt tgtcgagaca caaaatctaa tctaaaagta tccccatact tttagaaaga
     3421 cttaagtcga gttccccaac tcggcttttt tcttttcgtt tgaaacactc aaacacgtta
     3481 aaactattat accaacggat tttcatagaa acaagcaaac ttaaaagtat actttttata
     3541 ctttgctttt ttattacttt tttaactttt acttttcgac atgaaaggca taaaattatg
     3601 tgtaatatca atgcttgtta tcggtatgtt tgtctttttt taactttgct ttttcgactt
     3661 aattttgtaa aaagtttaat attattatta ctttttttac ttattttcga atgaatttac
     3721 tagtttatag aaagtagtcc gttttaactc taattcttcc atagcttcta ttgctttaat
     3781 ttctccatct ttccaacgct tgtacacttc ttcgaatcct ggcgggaatg tagcagccgg
     3841 tcttccgaac ttcgcacctc gtctttgtgc ggcatcaatg ccctcacgtt gtctctcttt
     3901 gattttccgt ctttcttctt ctgcaaagaa tgatagtatc tgtagtacca aatcacttac
     3961 aaacgagcct aatacatctt tatattgtct tgtatctaat aaaggcatgt ccaatacaac
     4021 gatatccgca ccgatctcct tcgttatttc attccactct tttaaaatat catctttatt
     4081 tctaccaaaa cgatcaatag atttgatata cagtatatct ccggcacgta gcatctgttt
     4141 taaagcctgg tatttaggac gattaaaatc tttaccgctt gccttgtcaa tgaagatatc
     4201 tctttcttca atctcctgct gcaacatctc gtccaactgt ctattttcgt tttgagtttt
     4261 atctgataca cggatataag cgaaacgttt gttcattttc ctcttcccct ttgttcgtaa
     4321 aagtgttttt caatttacga atgtttgtaa actaatcata caacttatac gaacacaaaa
     4381 caatatcttt ttcttattct ttttcttatt cgtaaatgtg tacttttacg aatacattaa
     4441 ttgagttcgt ttgttttgca aattactaaa atactactac attcattttg cactaatact
     4501 ccttatcatt ccttaacacc cgttaccatt caattaccgg tatatgtgct ttgttcttta
     4561 ttagttgagt tgtgtttgtt ttgcagtaca acattaaaag gggtgcaata ctacaatgag
     4621 tactgcataa aaagggtgcc ttgtgtgcgg attaaagtaa cgtggtattc gtgtttgttt
     4681 tgcaaaggta acgatattgg aacgctccgt aaaacgttct atattccaac ggagtagtat
     4741 acaaaattac tccattcaat gagatcttat tcacgagaaa aaagcgagtg cccagttata
     4801 cgcaccactt ttcttgtgcg tttctgagga tgttacctgc agggctttct gtttaatcta
     4861 cctctaagtg atccaaactc actttaaaat aaaaaaggac tccccatagt tagggaatcc
     4921 tacttccaaa acttgtacca tttcttttct tgactagcgg aagccgtttc taacaaaact
     4981 cgtttttctt cttgcatttc tcggatagct cccattaact gctgatctcg tttttctaac
     5041 ttttcatcaa tatacctctg ctgatcatct aatttcttcg ccattttctt taataattca
     5101 ttctgctgct gaatcatttc tttaagctca ctatatcgct cgtcatgttg ctcattttcc
     5161 gttataacat tcgtatcaac ctctgacata tctttctctt taacccacgt cataattgcg
     5221 ttacaggcct gttttaatgt catatcagga tgtgatttaa tctcgattaa ttttcttaac
     5281 gttataacat cgttatcaag gaatcctcta tggccgtgct cattcttatg aaatttgtag
     5341 ccgtgttcct caagcatgat acaatattta cgtaaagtcg attcttgaat tttaagcatc
     5401 atcgctacgt ctttgcttat atatatggat tgagagctgt ctgttttatc tgtcataacg
     5461 cttcacctct accaaaacat tcgttataac aatgatatat ccttttcctt aataattcac
     5521 gtatcttttt tgtctgctcc tattttagat ccaacttcga taactttgac atgtgtaata
     5581 tatttggttt gtattaacat tcctagatga tcaatcagta atgtcttttc tttaaggaat
     5641 agatcttcta acccttctaa ataatcttct ttgttgtatt tgcttgttac cttaccatca
     5701 aattcttttc cgttagaaaa ataaaaagtt acatggtatc tatgttcttc agccatttag
     5761 tccacctact ttcaaaatat atcagtagga ttccccaaga cagtataaaa aaaagacgtg
     5821 gatcaccacg ccttaattgt cctttaacca tgcttttact atatctaaat gattcatcgc
     5881 ttgttcatcg ccatgttgcg ctgctgatat gattttatgt aatagttctt cgaattcgtg
     5941 taaaaccatt tctacttcac tcataacgtt acaccttctt catgtcccgc ttctttaagt
     6001 gcttcatcta ctaatccaaa ccattcatca tgacgttttt gttgaataag tgtacataat
     6061 tcctctacta atttgaatga tttatcttcc gaaacacctt ttaacataca agcattcaca
     6121 aacttttcta cgatctcaaa tttatcttta gcaactgtat ttgccatgaa tattacctcc
     6181 gaattgtttt atttacatat aatcccgttt aacgcacttt ggaaacgtat gataattccc
     6241 tattaatgac cttaattaac gcttccgctg cattctcttt ggtgtaatct tcctcggtaa
     6301 gtaaatcatg tgcaatccaa gctaaatcct taactaattc agttttctca gcaagatgtc
     6361 ctaactgttc tttttgtggt aacatactaa tacatccttt ccataaggga taatgtgttt
     6421 tgaaagagta ggagccgcaa tctcctgctc tttctttgtt tttacagtgt tactttgtaa
     6481 ttgtttgttg ctgttccatt cctttgaacc tcgacgaatc ccttttcttc taactgtttt
     6541 acgagccttg taaccgtagc tctactcaaa ttcgtgtaat tctccaattc tgtaatagag
     6601 aatgggataa acttgttgtc tgaatagatc gctatcgtta gcaagattag tttttcagaa
     6661 gaagaaactt catctttgtc caattcaaga atacgatata gtgatttcat aattaaacta
     6721 gtaacctcct ataaatcaac atgttgttta cattatctag tataacaatg agtatagtaa
     6781 aatgcaatag aatcaaaata aaatttaaaa ataatataac catacatgta atttaacccg
     6841 ttatcgtttc atccaatctg gaatattcaa ctgtaaatct tctattccga ggttcttaat
     6901 taacttcctt cttaccggat ccatctgttt tttataattc tgtggtgtta tatccatcgc
     6961 aaggtaatgt tgcctatctg atttgtagtg atctttatat aaataccgct catagatcag
     7021 ctttctttcc tgcttattta acgaattaac agcatctaca acaaattgat agaacgaata
     7081 caatgaaacg tcgctcctaa cctcacagaa tcctctcaga cagtctataa aactttcata
     7141 atagttttgt ataggattat cataatttag cttgtttcgt ctcatacgtg gttctgtatc
     7201 gccttgcaaa cgtactgtat aaaaacaata cacgtgaaag atgtttttta atgtatcttt
     7261 actcatgaaa atccctccta ataaaaaaaa gccgccctta ggcgacttat gtattttgat
     7321 taatgagaat ccgacacagc gaaatactta ttgaaagcct tcatgaactg ctcatactct
     7381 ttatccgttt tgtctttgat tttctgtgct tcttcttcat atatcgtttc atattcatca
     7441 ttcaatttga acaggttatt atcgccaccc caacgtatca attcaccgcg ttgataccct
     7501 attcgtgtta gatttggttt gtcagcaaaa cgatgtatat cggcgaaagt acgagcgtcg
     7561 tcgatcactg tagcactaac atgcaaatac tttctactaa atcgaccttc atcttcaaag
     7621 gacgtcgccc tataaagatc cctgttaatt acttttttcg cttttctgca cttttcaggt
     7681 agttcgatta gcataacgtc ctgtttagcg gcatctacta attcagcatg caattcttcc
     7741 ttaacgatag ctgcaatctc atcagaaacc ttgttaagtt cttcttgagt agctattaac
     7801 tctgattgga acttctcttg agcttcttca tattttgcaa caaccttttt attagctgta
     7861 ctatcatcaa tcattaagtc agtttgtgcc agttccagag ctctttctac tcgagatagc
     7921 ttactttgta attctgattc tttggaattc aattcccctt ttgctattgt taattcgttt
     7981 aacttactct cgttatctac attgaagaac ttgctgattg tttgcatgca cgaacacttc
     8041 cttccacttt gttttttgac tcatctaatg tcactgtacc tttgaatatt tccaagcacg
     8101 cttctgtata agctgaatca cgcttatcta tatgcctttt tctgctcaca aggccttctt
     8161 taccaatctg ataatctatt acatcctgta caataaaaac gcccttaaat tcttttttag
     8221 acgttgtagc aataggttgt ttatatacag tcactaattc acttaaaata gcagatactg
     8281 cacgcttgtc ttttattcca ctacgtgcca taatttctt
//
