LOCUS       NZ_AP022973             8227 bp    DNA     circular CON 14-MAR-2024
DEFINITION  Bacillus cereus strain J75 plasmid pJ75_3, complete sequence.
ACCESSION   NZ_AP022973
VERSION     NZ_AP022973.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203337
            Sequence Read Archive: DRR206377, DRR206401
            Assembly: GCF_021655235.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8227)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 450x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655235.1-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 04:10:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,881
            CDSs (total)                      :: 5,729
            Genes (coding)                    :: 5,502
            CDSs (with protein)               :: 5,502
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 103 of 227
            Pseudo Genes (incomplete)         :: 133 of 227
            Pseudo Genes (internal stop)      :: 48 of 227
            Pseudo Genes (multiple problems)  :: 51 of 227
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022973.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="J75"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pJ75_3"
                     /geo_loc_name="Japan:Tochigi"
                     /collection_date="2006"
     gene            join(7486..8227,1..694)
                     /locus_tag="L6478_RS29175"
                     /pseudo=""
     CDS             join(7486..8227,1..694)
                     /locus_tag="L6478_RS29175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053955.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /name="IS4 family transposase"
     gene            complement(1379..1999)
                     /locus_tag="L6478_RS29180"
                     /old_locus_tag="BCJMU75_p32"
     CDS             complement(1379..1999)
                     /locus_tag="L6478_RS29180"
                     /old_locus_tag="BCJMU75_p32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000963189.1"
                     /translation="MAIDFSQADRNARKRDLEKEKFFSDEEWELANDLQSRANARGMKL
                     VPERKIKNRAKFAQMLQDNVDYLEEIDYLDDKEIIFLWKISRKVGFLSNCLVDDIHKKS
                     PVPLTQTDIATLLKRTKNNVNPIVKSLVAKGILAVSKTGVQGNNSRANVYFMNPNILYA
                     GSKDDVNLTLQAMFSKVPKALKDMPVKLFELSCHSEEEEEKTN"
                     /name="hypothetical protein"
     gene            complement(2250..2687)
                     /locus_tag="L6478_RS29185"
                     /old_locus_tag="BCJMU75_p33"
     CDS             complement(2250..2687)
                     /locus_tag="L6478_RS29185"
                     /old_locus_tag="BCJMU75_p33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000094076.1"
                     /translation="MSSFRWYEENEVIGKTIIEEDDLTLLEAGKIYIDAIFCERIRFPV
                     QKNEQPIGFNFVYEYNVKKYFGLYDRSFMKIIKQEDLEEYHMRMIDFHVHRLSLGEELD
                     DCPIIGICYGSGPLITPRLEQVYDVSKTKAYRKYRLEKLFD"
                     /name="hypothetical protein"
     gene            complement(2701..3108)
                     /locus_tag="L6478_RS29190"
                     /old_locus_tag="BCJMU75_p34"
     CDS             complement(2701..3108)
                     /locus_tag="L6478_RS29190"
                     /old_locus_tag="BCJMU75_p34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000037919.1"
                     /translation="MSKKISIEEMTQEMKEKFTISLEDAHKILLDHGVTKSTNTQVTMR
                     WIRDGKIDALLIGKGRVETRKWLINKESLMEFVAFERLTLGDFKAMKEELEMLRAFKFE
                     VEKKPKRATGTRQKKDAATNTSKEKKEVPTK"
                     /name="hypothetical protein"
     gene            complement(3921..5033)
                     /locus_tag="L6478_RS29195"
                     /old_locus_tag="BCJMU75_p35"
     CDS             complement(3921..5033)
                     /locus_tag="L6478_RS29195"
                     /old_locus_tag="BCJMU75_p35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002019875.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_000036645.1"
                     /translation="MSKITNDIDLKIDEVYEKVKDMKITFDENNTTKKQLTDSTINTYC
                     GIVKSYNNWLIQTHGISVDRAKPRHAMEYLETKIQAFEKGNASAFSMRQMAHAFHSFRV
                     ASAETGTFKHTCKVADKRDMLNTLTERGIFRKSADSTVLKANHSDYEKVQKELNVATTS
                     KHLREDIKTVHELQRYAGMRVHEAVKLKVSDIDFKSGTVYIKGKGGHERTVKIESKELL
                     EKLKNLCTGKSERSQVIIARKRDKNQLTNEEFGKRAKEEIRGAAVNAGVDRNGKRYTTH
                     SGRKAYAQAQMDKYKSYSKNQLKKEVAKRCQDKKTKQKYDAVIKNIRKKITEGSKNEGR
                     KLTNKEMCQFLVSLDLGHYRLDVVRYYADY"
                     /name="tyrosine-type recombinase/integrase"
     gene            6228..6779
                     /locus_tag="L6478_RS29200"
                     /old_locus_tag="BCJMU75_p36"
     CDS             6228..6779
                     /locus_tag="L6478_RS29200"
                     /old_locus_tag="BCJMU75_p36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_000028030.1"
                     /translation="MSISKHANKKKNILIAASEIVKEEGVVKLTLEAVAQRAGVSKGGL
                     LYHFPSKEALIKGMVEDWTNNYFECINTLVNNDDDNAIGKWNRAYLKSTFSDLENNNLN
                     SALMATMFINPDLLDEFRQRYDILHTKLITDGIDPVKITITRLSIDGLWFSEIFGMAPL
                     NEELKTQVFDELINMIQEDE"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            6779..7156
                     /gene="qacH"
                     /locus_tag="L6478_RS29205"
                     /old_locus_tag="BCJMU75_p37"
     ARG             6780..7153
                     /name="qacH"
                     /gene="qacH"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacH"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             6780..7156
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="60.75"
                     /coverage="116.82"
ORIGIN
        1 tatatccaag ttgatttaga acatattatg aacaatttaa aacccggtca aacatacgaa
       61 ataaaagaag cttacattgg aaagaatcaa aaactattca cacgtgtaat tatctatcgt
      121 ttaacagaca aacagataca ggaacgtaga aaaacaaagt tacactgaaa gtaaaaaggg
      181 tattacattt tcagaaaaga gtaaacgatt aacgggtatc aacatatatg tctcaaatac
      241 gcctgaagga gttgttccga tggaacaaat acatgacttt tactcccttc gctggcaaat
      301 agaaattatt tttaaaacgt ggaaatcctt atttcaaatt catcattggc aaactatcaa
      361 aaaagagcga ttagaatgtc atgtgtatgg aagacttatt gccatcttta tctgttcttc
      421 tacgatgttt aagatgcgta aacttctttt gcaaaagaac aaaagagaat taagtgaata
      481 taaagccatt gggatgattc aagatcatgt atcgctgtta tatcaagcga tacagagaaa
      541 cacccaaaat ctaacaaaga ttttaatccg tttgttcgac ctcctacaga agaatggacg
      601 gaaatctcat cgatatgaga agaaaactat ctttgatatt atgggtgttg tctatgagta
      661 taatgggttt ggaaaacaaa agaaagctgc ataattttta aaatgaaact cgttagagtt
      721 tatttggtgt gtgtactttt caataccaaa agtacattac gtgtttttta gtttgtttga
      781 acgaaagttc attttgtttt cgttcttaag ttgatgggca tgtgacgcta cccctttcct
      841 aagcaaaaat gtttttaagt cttttattct tttaccgtat taaatttgat acggttttca
      901 tatgcttttt tgggttgagc tggctggggt ttggggctag ccccaagggt ttttattaca
      961 caaaatattg atagaacgga aatgtacgca ctaagcgacg gagcaattaa aatggattgt
     1021 tcaagtgata atccgaaaaa gtgataactt ttttggttta tcgcttggca caagccatgc
     1081 caattgcgga gaattttgag tcaagataaa gagtggagat tttttgcggt ttttgcaaaa
     1141 aatactactc gtttctttac tcaaaattag cgttaataga gagtgagata gaacaacatt
     1201 gttaatgtat taaaaagtca ataatatcaa tggtttgttg cacttttcgt agttcttgaa
     1261 tttaagaacc atagttcttg aatttaagaa caccggattt tttacatttt ttacattatt
     1321 ttacataatt gaaagataaa aaaatacatg tattaaaaaa tacatgtatt aattattatt
     1381 aatttgtttt ctcttcttct tcttctgaat gacatgatag ttcgaataat tttacaggca
     1441 tatcctttaa tgctttagga acttttgaaa acatcgcttg taatgttaaa ttgacatcat
     1501 ctttgctacc ggcgtacaaa atgttcggat tcataaaata cacatttgct ctcgaattat
     1561 taccttgaac acctgttttt gaaacagcca gtatgccttt tgctactaaa gatttaacaa
     1621 taggattgac attattttta gttcttttca acaaagttgc gatatctgtt tgtgttaaag
     1681 gaactggact ttttttatga atatcatcca caagacaatt tgataaaaaa ccaactttac
     1741 gactgatttt ccataaaaat ataatctctt tatcatctaa ataatcaatc tcttcgagat
     1801 aatcaacgtt atcctgaagc atttgagcaa attttgctcg attcttgatt ttacgctctg
     1861 gaacaagttt cattcctcgc gcgttagctc ttgattgcaa atcatttgct aattcccatt
     1921 cttcatctga aaaaaacttt tctttttcta agtctctttt tctagcgttt cgatccgctt
     1981 gactaaaatc aattgccatt gattttgctc ctctcctgaa gaaaatgcaa caagcattct
     2041 aggagattca ccgttatttt tttattatcc gtaaagggtt ttacccttga tttttaaatg
     2101 aaaattcatt aaaattaaaa ctagaaatca acgttttggt gataaattac gatgaatcac
     2161 ccaaagtcga gtgctccaac actcggcttt tttttatgct tgtctattca attctagctt
     2221 aaaaactcaa attttgtaag atgctttacc taatcgaata atttttctaa tcgatacttt
     2281 ctgtatgctt ttgttttcga aacatcataa acttgttcca acctcggtgt tatcaaaggt
     2341 ccacttccat aacaaatacc aatgattgga caatcatcta attcttcccc taaagataat
     2401 ctgtgcacat gaaaatctat cattctcatg tgatattctt ctaaatcttc ttgtttaatt
     2461 atcttcatga aacttctgtc atataatccg aaatattttt ttacattata ttcataaaca
     2521 aaattaaatc caattggctg ttcgtttttt tgaactggaa accttattct ctcacaaaat
     2581 attgcatcaa tatatatttt cccagcttct agcaaagtca aatcatcttc ttctattata
     2641 gtttttccaa tcacttcatt ttcttcatac catctaaaag atgacatgca attcacctct
     2701 ttattttgtt ggcacttctt tcttttcttt tgaagtattt gttgctgcat ctttcttttg
     2761 acgtgtacca gtagcacgct ttggcttttt ctctacttcg aatttaaaag cacgtaacat
     2821 ttctaattct tctttcattg ctttgaaatc acctaacgtt aaacgctcga atgcaacgaa
     2881 ttccattaga ctttccttgt taataagcca ttttcgagtc tccacacgac cttttccaat
     2941 caataatgca tcaattttcc catcacgaat ccaacgcatt gtaacttgtg tattcgtact
     3001 tttcgttacg ccatgatcca aaaggatttt atgtgcatct tccagtgaaa tcgtgaattt
     3061 ttccttcatt tcttgcgtca tttcttcaat tgagattttt tttgacattg ttttttcctc
     3121 ctaattacaa ttaagtacat gtatttttaa ttaaatgtat ccatctatat ttttaatata
     3181 cagtaaatgt attgaaaagt aaatataatt ttaaataaat ttattaaaaa atatatttaa
     3241 tctattttac atttactatt aattatattt actcaaaatt atatatatcc ttctttaaat
     3301 ttattataca ttaaatttat ttttatgtaa atccatttta ggtcattact tttacttttg
     3361 tgtaaatcta tttattgcca tgactttact attttgtaca tgtattttat atctcttaaa
     3421 cgttgttaaa tcaacgattt tttgcgatta ctaaaaataa attgcggaga caatacatgt
     3481 atttttatct atatttttac ttttgtgtaa atctaattta ggtatataaa tttactattg
     3541 tgtaaaacta ttaaaattac tttaaaatac ttttactcaa aaataaattt actatacaat
     3601 aaatgtattt taaaatactt ttactgtata gtaaatttaa taggcgtttc attttttttt
     3661 gattttcgaa aaaaagcgag aaatggcgag gatttggaaa actctcgtgt atcttcttag
     3721 ttcatcgacc cgttttttgg cgatttcctc gaatccgcac aaggtactgg cattgccatg
     3781 agaggctaac acaaggcttt ctcattgtaa agccgggaaa gatggcttga caacgaggct
     3841 ctagcccttg tgtttctaag ccagtgcgcc agtaccaact gtgctccgca ctcgtcttcc
     3901 cgattttccg tcaaaaccta tcaataatca gcataatatc gtacaacatc taaacgatag
     3961 tgacccaaat ccaagctaac tagaaactgg cacatctctt tatttgtaag tttacgccct
     4021 tcgttcttcg aaccttcggt tattttttta cggatatttt ttataactgc atcatatttt
     4081 tgctttgttt tcttgtcctg acaacgtttt gcaacttctt tttttaattg attcttactg
     4141 taacttttgt atttatccat ttgcgcttgt gcatatgctt tgcgacctga atgtgttgtg
     4201 tatctttttc cgttacgatc caccccagca ttgacggctg cacctcgtat ttcttctttt
     4261 gcacgttttc caaattcctc atttgttaat tgatttttat cacgttttcg agcaataatt
     4321 acctggcttc tttcactttt tccagtgcat aaatttttca acttctctag taattcttta
     4381 ctttcaattt taaccgttct ctcgtgcccg cctttgcctt ttatatagac agtgccactc
     4441 ttaaagtcta tatcactcac ttttagtttt acggcttcgt gaacacgcat tccagcgtac
     4501 cgctgtaatt cgtgtactgt ttttatatct tctctcaaat gtttacttgt cgttgctaca
     4561 tttagttctt tttgaacttt ttcatagtct gaatgatttg cctttaatac cgtcgaatca
     4621 gccgatttac ggaagattcc acgctctgtg agtgtattta acatatctcg tttatcagcg
     4681 accttacatg tatgtttaaa cgttcctgtt tcagcactcg caacacgaaa agaatgaaac
     4741 gcatgcgcca tttggcgcat agaaaatgcg gaagcatttc ctttttcaaa ggcttgaatt
     4801 tttgtttcga gatattccat tgcgtggcga ggtttcgccc tatccacgct aattccatgc
     4861 gtttgtatta accaattgtt atatgatttc acgattccgc aatatgtgtt tattgttgaa
     4921 tcagttaatt gttttttggt tgtattattt tcatcaaaag ttattttcat atctttaact
     4981 ttttcataga cttcatcaat ttttaaatca atatcatttg taattttgct catttcgtca
     5041 ccccctatat acaaaatcaa gagcaagaac ccaacccccg tgaaccttac aatatcaagg
     5101 cacaacaaaa acgaggtctc atttgggtgg gttcgatcta ttttttttaa agaccaaaaa
     5161 aaatagaccg aaccacccaa attaaacaca caaaagcaat cgcgaaatct cccttcgggc
     5221 gatttaagct cactagagtc aaagtcaaaa tcaaaagacc aaaagcaaaa atcaaggtca
     5281 aaagattaaa agacaaaggc tcaagatagc tacgccatct tgttgcgatt ggcgtctata
     5341 aatagactcc aaggctctcc catacatttt gcaccaaaat gtatagtcaa atgagcgtac
     5401 aacggtattg cggtctggaa tttttgagta acccagaaat tactgttctt gcgatttgtt
     5461 ttgtggttaa aaacctttgt tacagattta catttattca gttgtaaaaa tatttaattg
     5521 taaaacttgt tcccttcgta ttttttgatt tgtcatctcc ttaaattttt attttttata
     5581 atattttttt ccattcttgc aagcaagaat tctattttct tttaaagcta tttataaaac
     5641 tacttgggaa agctttatat tgctcctttc ttagattatt ttttcccacg tcttaaaaac
     5701 attttgcttt gctattgtaa tgatcccctt tcatttttat gacgtgaatt ttttagtttt
     5761 gtaaaataca cgattacttg aatcacgtcc caagattcat agtataaaat ttcgtatttc
     5821 atatttgctt aaccgcaagc gtttaaacaa ataagtaatt ggttttaaaa ttactatgaa
     5881 tatatacaaa ttttaccatt aatcaactga ttttgatgaa aaaaccttat ttttttatgc
     5941 ttttgaaaag gtcttacgac cttaaatgtt tttctttccg gggtagagtc ctgaaaatgc
     6001 ggatttacaa cggtaatgta attttcaccc ataaataaag ccgattttaa gaaatcgact
     6061 gttgaactaa agctcccttt actttaagta catttcttca caatctttag attaatttat
     6121 tggattattt ttatgtgaaa gagtgatatt aaataagagc tttaattcga ttgacaaaaa
     6181 aaccgtccag acggtatatt atatgtatct aagttatgga ggttattatg tctatttcaa
     6241 agcatgctaa taagaaaaaa aatatattga ttgctgcatc tgaaattgta aaagaagaag
     6301 gagttgtcaa actcacatta gaggcagtcg cccaaagagc tggcgtgagt aaaggtggat
     6361 tattatacca ttttcctagc aaggaagcat taattaaagg aatggtggaa gattggacaa
     6421 ataattattt cgaatgcatt aatacgttag ttaataacga cgatgataac gcaataggaa
     6481 aatggaatag ggcttatctt aaatctacat tttccgattt agaaaacaat aatctaaatt
     6541 ccgcactaat ggctactatg tttattaatc cagatttatt agatgaattc cggcagagat
     6601 acgatatttt gcatacaaag ttaataactg atggaattga tcctgtcaaa ataacaatta
     6661 caagattatc catagatggt ctttggtttt cagaaatatt tggaatggca ccattaaatg
     6721 aagaactaaa aacacaagtt tttgatgaac taataaacat gattcaggag gacgaataat
     6781 gtcatatcta tatttagcac ttgcaatagt gggggagatt attggctctt ccctattaaa
     6841 agcatctgaa ggcttttcaa aactctatcc tacaataggg gtaataatag ctttcgttgg
     6901 ttgttttttc tttttatcct tgtcgctaaa aacaatcccc ttaaacactg catatgcatt
     6961 atgggcaggt ttaggacttg ttttgactac agtaatttca gttttagttt ggaaggaaaa
     7021 acttaacatg gctagcattg caggaataac actgattctt gtaggagttg taatattgaa
     7081 tttgtttgga ccaggacatg gagaatctgg tcatgaagca agcgaaacta ttacaatgaa
     7141 tgagacaatg gaataacaca taaaaaagtc cttcttaata aagagaagga ctttttagct
     7201 taattaaggg tattttttga agtccttatt caacaaacct gcgtacgtta gtttaagtgt
     7261 atcacttcac aattccaata gagtagagtt tattgagaca taatgaaatt agcgttttaa
     7321 atgacttctg actgtctcat aagacttgta tcaaatgctt accgttgtaa atccgcattt
     7381 ccctgacgct accccatgcc catcaactta agaattttca ctacccccaa gtgacaaaaa
     7441 acgttattct ttctaaaaag ctagctagaa agggtgacat tttttatgaa tctttcgatt
     7501 caagatgaat tacaactatt ttctgaagaa ctgtatcgtc atttaacccc ttctcttttg
     7561 gaagaactcg caaaagaatt aggttttgta aaaagaaaac gaaagttttc tggaaatgaa
     7621 ttagctacca tatgtatttg ggtaagtcaa cgtacagcga gtgactcgct tgttcgacta
     7681 tgcagtcaat tacatgcggc cacaggcact cttatgagtc cagaaggact caataaacgc
     7741 tttgataaaa aagcggttga atttttgaaa tatatttttt cggcattatg gaaaagtaaa
     7801 atctgtgaaa catcggccat ttcaagcgcg gcgctcacgt attttcaacg aattcgtatt
     7861 ttagatgcga cgattttcca agtaccaaaa catttagccc atgtatatcc tggatcaggt
     7921 ggttgtgcac aaacagcggg tatcaagatt caattagaat atgacttaca tagcggacag
     7981 tttctaaact ttcagatagg tccaggaaaa aataatgata agacctttgg aacagaatgc
     8041 ttagacacat tacgtccaag cgatttatgt atccgtgatt taggttattt ttcattagaa
     8101 gacttagatc aaatggatca acgaggtgct tattatatat ccagattaaa attaaaccat
     8161 accgtgtatg taaaaaatcc gtttccagaa tactttcgaa acggaaccgt aaaaaaagca
     8221 atcacag
//
