LOCUS       NZ_AP022972             8319 bp    DNA     circular CON 14-MAR-2024
DEFINITION  Bacillus cereus strain J75 plasmid pJ75_2, complete sequence.
ACCESSION   NZ_AP022972
VERSION     NZ_AP022972.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203337
            Sequence Read Archive: DRR206377, DRR206401
            Assembly: GCF_021655235.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8319)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 450x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655235.1-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 04:10:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,881
            CDSs (total)                      :: 5,729
            Genes (coding)                    :: 5,502
            CDSs (with protein)               :: 5,502
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 103 of 227
            Pseudo Genes (incomplete)         :: 133 of 227
            Pseudo Genes (internal stop)      :: 48 of 227
            Pseudo Genes (multiple problems)  :: 51 of 227
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022972.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8319
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="J75"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pJ75_2"
                     /geo_loc_name="Japan:Tochigi"
                     /collection_date="2006"
     gene            27..176
                     /locus_tag="L6478_RS29105"
                     /old_locus_tag="BCJMU75_p201"
     CDS             27..176
                     /locus_tag="L6478_RS29105"
                     /old_locus_tag="BCJMU75_p201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788727.1"
                     /translation="MEREKCSLKEAKNAYNRKWRAANRDKVKLYNDRYWEKKAKEMEEQ
                     ACQK"
                     /name="hypothetical protein"
     gene            164..436
                     /locus_tag="L6478_RS29110"
                     /old_locus_tag="BCJMU75_p202"
     CDS             164..436
                     /locus_tag="L6478_RS29110"
                     /old_locus_tag="BCJMU75_p202"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788729.1"
                     /translation="MSEVSMETVIKGKQQSELLKHLEKVGIELLGRRDELLEQWEKEGL
                     KEDSIFEDDLKFVEELMNRNDELMFDVKVELITTMDEIHHQKMGY"
                     /name="hypothetical protein"
     gene            441..692
                     /locus_tag="L6478_RS29115"
                     /old_locus_tag="BCJMU75_p203"
     CDS             441..692
                     /locus_tag="L6478_RS29115"
                     /old_locus_tag="BCJMU75_p203"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788731.1"
                     /translation="MLGKYDGLLEKERLLKRIDELSFALEASGDPMLEAMVKNSGNTDI
                     AVAKELITKGSELGMNEHVFKKALLAKVNELREEVTKL"
                     /name="hypothetical protein"
     gene            689..1102
                     /locus_tag="L6478_RS29120"
                     /old_locus_tag="BCJMU75_p204"
     CDS             689..1102
                     /locus_tag="L6478_RS29120"
                     /old_locus_tag="BCJMU75_p204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788733.1"
                     /translation="MKKDKQKDLPFNHKVVLSVLPLGRSKAITTKEIMARSGIKDKRAV
                     SAILSELVTVYKQPIATTSKKEFKGVFIVQDVIDYQIGKEGLVSRKRHIDKRDSAYTEA
                     CLEIFKGTVTLDESKNKVEGSVRACKQSASSSM"
                     /name="hypothetical protein"
     gene            1072..1779
                     /locus_tag="L6478_RS29125"
                     /old_locus_tag="BCJMU75_p205"
     CDS             1072..1779
                     /locus_tag="L6478_RS29125"
                     /old_locus_tag="BCJMU75_p205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788735.1"
                     /translation="MQTISKFFNVDNESKLNELTIAKGELNSKESELQSKLSRVERALE
                     LAQTDLMIDDSTANKKVVAKYEEAQEKFQSELIATQEELNKVSDEIAAIVKEELHAELV
                     DAAKQDVMLIELPEKCRKAKKVINRDLYRATSFEDEGRFSRKYLHVSATVIDDARTFAD
                     IHRFADKPNLTRIGYQRGELIRWGGDNNLFKLNDEYETIYEEEAQKIKDKTDKEYEQFM
                     KAFNKYFAVSDSH"
                     /name="hypothetical protein"
     gene            1833..2258
                     /locus_tag="L6478_RS29130"
                     /old_locus_tag="BCJMU75_p206"
     CDS             1833..2258
                     /locus_tag="L6478_RS29130"
                     /old_locus_tag="BCJMU75_p206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788712.1"
                     /translation="MSKDTLKNIFHVYCFYTVRLQGDTEPRMRRNKLNYDNPIQNYYES
                     FIDCLRGFCEVRSDVSLYSFYQFVVDAVNSLNKQERKLIYERYLYKDHYKSDRQHYLAM
                     DITPQNYKKQMDPVRRKLIKNLGIEDLQLNIPDWMKR"
                     /name="hypothetical protein"
     gene            2389..2637
                     /locus_tag="L6478_RS29135"
                     /old_locus_tag="BCJMU75_p207"
     CDS             2389..2637
                     /locus_tag="L6478_RS29135"
                     /old_locus_tag="BCJMU75_p207"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_236788716.1"
                     /translation="MKSLYRILELDKDEVSSSEKLILLTIAIYSDNKFIPFSITELENY
                     TNLSRATVTRLVKQLEEKGFVEVQRNGTATNNYKVTL"
                     /name="helix-turn-helix domain-containing protein"
     gene            2714..2890
                     /locus_tag="L6478_RS29140"
                     /old_locus_tag="BCJMU75_p208"
     CDS             2714..2890
                     /locus_tag="L6478_RS29140"
                     /old_locus_tag="BCJMU75_p208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176543923.1"
                     /translation="MLPQKEQLGHLAEKTELVKDLAWIAHDLLTEEDYTKENAAEALIK
                     VINRELSYVSKVR"
                     /name="hypothetical protein"
     gene            2932..3138
                     /locus_tag="L6478_RS29145"
                     /old_locus_tag="BCJMU75_p209"
     CDS             2932..3138
                     /locus_tag="L6478_RS29145"
                     /old_locus_tag="BCJMU75_p209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788718.1"
                     /translation="MANTVAKDKFEIVEKFVNACMLKGVSEDKSFKLVEELCTLIQQKR
                     HDEWFGLVDEALKEAGHEEGVTL"
                     /name="hypothetical protein"
     gene            3135..3266
                     /locus_tag="L6478_RS29410"
                     /old_locus_tag="BCJMU75_p210"
     CDS             3135..3266
                     /locus_tag="L6478_RS29410"
                     /old_locus_tag="BCJMU75_p210"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272880692.1"
                     /translation="MSEVEMVLHEFEELLHKIISAAQHGDEQAMNHLDIVKAWLKDN"
                     /name="hypothetical protein"
     gene            3343..3582
                     /locus_tag="L6478_RS29150"
                     /old_locus_tag="BCJMU75_p211"
     CDS             3343..3582
                     /locus_tag="L6478_RS29150"
                     /old_locus_tag="BCJMU75_p211"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788720.1"
                     /translation="MAEEHRYHVTFYFSNGKEFDGKVTSKYNKEDYLEGLEDLFLKEKT
                     LLIDHLGMLIQTKYITHVKVIEVGSKIGADKKDT"
                     /name="hypothetical protein"
     gene            3643..4179
                     /locus_tag="L6478_RS29155"
                     /old_locus_tag="BCJMU75_p212"
     CDS             3643..4179
                     /locus_tag="L6478_RS29155"
                     /old_locus_tag="BCJMU75_p212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3967 domain-containing protein"
                     /protein_id="WP_236788722.1"
                     /translation="MTDKTDSSQSIYISKDVAMMLKIQESTLRKYCIMLEEHGYKFHKN
                     EHGHRGFLDNDVITLRKLIEIKSHPDMTLKQACNAIMTWVKEKDMSEVDTNVITENEQH
                     DERYSELKEMIQQQNELLKKMAKKLDDQQRYIDEKLEKRDQQLMGAIREMQEEKRVLLE
                     TASASQEKKWYKFWK"
                     /name="DUF3967 domain-containing protein"
     gene            4803..5402
                     /locus_tag="L6478_RS29160"
                     /old_locus_tag="BCJMU75_p213"
     CDS             4803..5402
                     /locus_tag="L6478_RS29160"
                     /old_locus_tag="BCJMU75_p213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562260.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_236788723.1"
                     /translation="MNKRFAYIRVSDKTQNENRQLDEMLQQEIEERDIFIDKASGKDFN
                     RPKYQALKQMLRAGDILYIKSIDRFGRNKDDILKEWNEITKEIGADIVVLDMPLLDTRQ
                     YKDVLGSFVSDLVLQILSFFAEEERRKIKERQREGIDAAQRRGAKFGRPAATFPPGFEE
                     VYKRWKDGEIKAIEAMEELELKRTTFYKLVNSFENK"
                     /name="recombinase family protein"
     gene            6158..6355
                     /locus_tag="L6478_RS29165"
                     /old_locus_tag="BCJMU75_p214"
     CDS             6158..6355
                     /locus_tag="L6478_RS29165"
                     /old_locus_tag="BCJMU75_p214"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788725.1"
                     /translation="MIGKDMITLDNPKKEYFQQYAQNNKEQINANARRRRQQQKEKVEK
                     YDRLVAALRSRGISVEELLK"
                     /name="hypothetical protein"
     gene            6778..7878
                     /locus_tag="L6478_RS29170"
                     /old_locus_tag="BCJMU75_p215"
     CDS             6778..7878
                     /locus_tag="L6478_RS29170"
                     /old_locus_tag="BCJMU75_p215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205124.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_098492605.1"
                     /translation="MNHEEFINSTEIDDKFLKQIIDNREFNDVIVSYYERLHKESRDDG
                     MKKKQENVKGCNDFWIIDKYHEHRLKEFKRTNLCKDKFCNNCKKVKQASRMGKFIPLIQ
                     PYAENMYQLTLTIPNVKGDELKDTLSKMQKAFARLIEYFKGKKKINGIDFEVLQYEGAI
                     RSLEITFNGNEYHPHYHALIVLKVNKFHDCMIRKHKNVYSIDFTGKREARLFSDIEVLI
                     QKVWYLIINGKRITETAIQSLKKGYSCQLDKFSENDFVEIFKYMTKATTEDGKTLSYKQ
                     FKTLYYALFKVRQIQGYGIFYNLKDDEEINFDEVNEAYAEFIEELQTKEKPVTVSEAPE
                     QVRKESDYTVFSRKTFQKYWKQVMSE"
                     /name="protein rep"
ORIGIN
        1 aagaagaatg aaaagaaggt ggaaaagtgg aaagagaaaa gtgttctttg aaagaagcta
       61 aaaacgctta taaccgaaaa tggagagcag cgaatcgcga taaagtgaag ctgtataacg
      121 atagatactg ggaaaagaaa gctaaagaaa tggaggaaca agcatgtcag aagtaagtat
      181 ggaaactgtt attaaaggaa agcagcagag tgaactgttg aagcatttag aaaaagtagg
      241 tattgaatta ctggggcgta gagatgaatt gttagagcag tgggaaaaag aaggacttaa
      301 agaagacagc atttttgaag atgatcttaa attcgttgaa gaattaatga accgaaatga
      361 cgaattaatg tttgatgtaa aagtggaatt aattacaact atggatgaga ttcatcatca
      421 gaaaatgggg tattgatagg atgttaggaa agtatgatgg attattagaa aaagaacggt
      481 tacttaaacg aattgatgaa ctgtcattcg cgttagaagc ttcaggtgac cctatgttgg
      541 aagcgatggt taaaaatagt ggcaatacag acatcgcggt tgcaaaagag ttgattacaa
      601 aagggagtga attaggtatg aatgagcacg tatttaaaaa agcgttgcta gcaaaagtaa
      661 atgaacttcg tgaagaggta acgaaactat gaaaaaagac aaacaaaaag acctcccttt
      721 taatcacaaa gtagtattga gcgtactacc tttaggaaga tctaaagcaa taacaactaa
      781 agaaattatg gcacgtagtg gaataaaaga caagcgtgca gtatctgcta ttttaagtga
      841 attagtgact gtatataaac aacctattgc tacaacgtct aaaaaagaat ttaagggcgt
      901 ttttattgta caggatgtaa tagattatca gattggtaaa gaaggccttg tgagcagaaa
      961 aaggcatata gataagcgtg attcagctta tacagaagcg tgcttggaaa tattcaaagg
     1021 tacagtgaca ttagatgagt caaaaaacaa agtggaagga agtgttcgtg catgcaaaca
     1081 atcagcaagt tcttcaatgt agataacgag agtaagttaa acgaattaac aatagcaaaa
     1141 ggggaattga attccaaaga atcagaatta caaagtaagc tatctcgagt agaaagagct
     1201 ctggaactgg cacaaactga cttaatgatt gatgatagta cagctaataa aaaggttgtt
     1261 gcaaaatatg aagaagctca agagaagttc caatcagagt taatagctac tcaagaagaa
     1321 cttaacaagg tttctgatga gattgcagct atcgttaagg aagaattgca tgctgaatta
     1381 gtagatgccg ctaaacagga cgttatgcta atcgaactac ctgaaaagtg cagaaaagcg
     1441 aaaaaagtaa ttaacaggga tctttatagg gcgacgtcct ttgaagatga aggtcgattt
     1501 agtagaaagt atttgcatgt tagtgctaca gtgatcgacg acgctcgtac tttcgccgat
     1561 atacatcgtt ttgctgacaa accaaatcta acacgaatag ggtatcaacg cggtgaattg
     1621 atacgttggg gtggcgataa taacctgttc aaattgaatg atgaatatga aacgatatat
     1681 gaagaagaag cacagaaaat caaagacaaa acggataaag agtatgagca gttcatgaag
     1741 gctttcaata agtatttcgc tgtgtcggat tctcattaat caaaatacat aagtcgccta
     1801 agggcggctt ttttttatta ggagggattt tcatgagtaa agatacatta aaaaacatct
     1861 ttcacgtgta ttgtttttat acagtacgtt tgcaaggcga tacagaacca cgtatgagac
     1921 gaaacaagct aaattatgat aatcctatac aaaactatta tgaaagtttt atagactgtc
     1981 tgagaggatt ctgtgaggtt aggagcgacg tttcattgta ttcgttctat caatttgttg
     2041 tagatgctgt taattcgtta aataagcagg aaagaaagct gatctatgag cggtatttat
     2101 ataaagatca ctacaaatca gataggcaac attaccttgc gatggatata acaccacaga
     2161 attataaaaa acagatggat ccggtaagaa ggaagttaat taagaacctc ggaatagaag
     2221 atttacagtt gaatattcca gattggatga aacgataacg ggttaaatta catgtatggt
     2281 tatattattt ttaaatttta ttttgattct attgcatttt actatactca ttgttatact
     2341 agataatgta aacaacatgt tgatttatag gaggttacta gtttaattat gaaatcacta
     2401 tatcgtattc ttgaattgga caaagatgaa gtttcttctt ctgaaaaact aatcttgcta
     2461 acgatagcga tctattcaga caacaagttt atcccattct ctattacaga attggagaat
     2521 tacacgaatt tgagtagagc tacggttaca aggctcgtaa aacagttaga agaaaaggga
     2581 ttcgtcgagg ttcaaaggaa tggaacagca acaaacaatt acaaagtaac actgtaaaaa
     2641 caaagaaaga gcaggagatt gcggctccta ctctttcaaa acacattatc ccttatggaa
     2701 aggatgtatt agtatgttac cacaaaaaga acagttagga catcttgctg agaaaactga
     2761 attagttaag gatttagctt ggattgcaca tgatttactt accgaggaag attacaccaa
     2821 agagaatgca gcggaagcgt taattaaggt cattaatagg gaattatcat acgtttccaa
     2881 agtgcgttaa acgggattat atgtaaataa aacaattcgg aggtaatatt catggcaaat
     2941 acagttgcta aagataaatt tgagatcgta gaaaagtttg tgaatgcttg tatgttaaaa
     3001 ggtgtttcgg aagataaatc attcaaatta gtagaggaat tatgtacact tattcaacaa
     3061 aaacgtcatg atgaatggtt tggattagta gatgaagcac ttaaagaagc gggacatgaa
     3121 gaaggtgtaa cgttatgagt gaagtagaaa tggttttaca cgaattcgaa gaactattac
     3181 ataaaatcat atcagcagcg caacatggcg atgaacaagc gatgaatcat ttagatatag
     3241 taaaagcatg gttaaaggac aattaaggcg tggtgatcca cgtctttttt ttatactgtc
     3301 ttggggaatc ctactgatat attttgaaag taggtggact aaatggctga agaacataga
     3361 taccatgtaa ctttttattt ttctaacgga aaagaatttg atggtaaggt aacaagcaaa
     3421 tacaacaaag aagattattt agaagggtta gaagatctat tccttaaaga aaagacatta
     3481 ctgattgatc atctaggaat gttaatacaa accaaatata ttacacatgt caaagttatc
     3541 gaagttggat ctaaaatagg agcagacaaa aaagatacgt gaattattaa ggaaaaggat
     3601 atatcattgt tataacgaat gttttggtag aggtgaagcg ttatgacaga taaaacagac
     3661 agctctcaat ccatatatat aagcaaagac gtagcgatga tgcttaaaat tcaagaatcg
     3721 actttacgta aatattgtat catgcttgag gaacacggct acaaatttca taagaatgag
     3781 cacggccata gaggattcct tgataacgat gttataacgt taagaaaatt aatcgagatt
     3841 aaatcacatc ctgatatgac attaaaacag gcctgtaacg caattatgac gtgggttaaa
     3901 gagaaagata tgtcagaggt tgatacgaat gttataacgg aaaatgagca acatgacgag
     3961 cgatatagtg agcttaaaga aatgattcag cagcagaatg aattattaaa gaaaatggcg
     4021 aagaaattag atgatcagca gaggtatatt gatgaaaagt tagaaaaacg agatcagcag
     4081 ttaatgggag ctatccgaga aatgcaagaa gaaaaacgag ttttgttaga aacggcttcc
     4141 gctagtcaag aaaagaaatg gtacaagttt tggaagtagg attccctaac tatggggagt
     4201 ccttttttat tttaaagtga gtttggatca cttagaggta gattaaacag aaagccctgc
     4261 aggtaacatc ctcagaaacg cacaagaaaa gtggtgcgta taactgggca ctcgcttttt
     4321 tctcgtgaat aagatctcat tgaatggagt aattttgtat actactccgt tggaatatag
     4381 aacgttttac ggagcgttcc aatatcgtta cctttgcaaa acaaacacga ataccacgtt
     4441 actttaatcc gcacacaagg cacccttttt atgcagtact cattgtagta ttgcacccct
     4501 tttaatgttg tactgcaaaa caaacacaac tcaactaata aagaacaaag cacatatacc
     4561 ggtaattgaa tggtaacggg tgttaaggaa tgataaggag tattagtgca aaatgaatgt
     4621 agtagtattt tagtaatttg caaaacaaac gaactcaatt aatgtattcg taaaagtaca
     4681 catttacgaa taagaaaaag aataagaaaa agatattgtt ttgtgttcgt ataagttgta
     4741 tgattagttt acaaacattc gtaaattgaa aaacactttt acgaacaaag gggaagagga
     4801 aaatgaacaa acgtttcgct tatatccgtg tatcagataa aactcaaaac gaaaatagac
     4861 agttggacga gatgttgcag caggagattg aagaaagaga tatcttcatt gacaaggcaa
     4921 gcggtaaaga ttttaatcgt cctaaatacc aggctttaaa acagatgcta cgtgccggag
     4981 atatactgta tatcaaatct attgatcgtt ttggtagaaa taaagatgat attttaaaag
     5041 agtggaatga aataacgaag gagatcggtg cggatatcgt tgtattggac atgcctttat
     5101 tagatacaag acaatataaa gatgtattag gctcgtttgt aagtgatttg gtactacaga
     5161 tactatcatt ctttgcagaa gaagaaagac ggaaaatcaa agagagacaa cgtgagggca
     5221 ttgatgccgc acaaagacga ggtgcgaagt tcggaagacc ggctgctaca ttcccgccag
     5281 gattcgaaga agtgtacaag cgttggaaag atggagaaat taaagcaata gaagctatgg
     5341 aagaattaga gttaaaacgg actactttct ataaactagt aaattcattc gaaaataagt
     5401 aaaaaaagta ataataatat taaacttttt acaaaattaa gtcgaaaaag caaagttaaa
     5461 aaaagacaaa cataccgata acaagcattg atattacaca taattttatg cctttcatgt
     5521 cgaaaagtaa aagttaaaaa agtaataaaa aagcaaagta taaaaagtat acttttaagt
     5581 ttgcttgttt ctatgaaaat ccgttggtat aatagtttta acgtgtttga gtgtttcaaa
     5641 cgaaaagaaa aaagccgagt tggggaactc gacttaagtc tttctaaaag tatggggata
     5701 cttttagatt agattttgtg tctcgacaac actttcgact aattagcact accaatgctt
     5761 attagtcatt gatgatttta cgaatcatct taacgattat ttttttatgg attgacacct
     5821 attttatcga cctttgacaa tcaaataatt aggtaccatt tatatctaag attgaattaa
     5881 caaccttatt taacgtatga aaaagtactg taggtcaagt tgtaaactat aaaaagtttc
     5941 ttcttaggaa accttaagag aaaaaaatat agatacaatt tgtatcgctt taatacgaaa
     6001 aaaagttctc gttcagcgaa cttcctatgt gacaagttta caatgtattt ttcgttttga
     6061 caatacttta agtaaactca tagtttttac atacattgaa aggtacctct actatttgag
     6121 gtgccttttt tatttatttt tgaaaggatg atagaaaatg atagggaaag atatgatcac
     6181 attagacaac ccaaagaaag agtattttca gcaatatgca caaaataata aagaacaaat
     6241 taacgccaat gcaagaagac gaagacaaca acaaaaagaa aaggtagaga agtatgatag
     6301 attggttgcg gcgttacgat ctagaggaat tagtgtagaa gaattattga aataagatgg
     6361 acaagctgta ttaaggccgg actaagacac caggagaaga tttgtatacg gtctaggtta
     6421 ttcgtggggc ttatgctaca gctgtcattg tctagcggta tcgcagtgcg taagcacgag
     6481 aataaggagc agacatacac cacaagcccc gaacactttc atgctctcca aaacaacatg
     6541 ttttggggat acctagtgta acgtggccga gtgcaacgga aggttttaat gtttcgttta
     6601 aaaaatgagt ttcttctatt acttgatata ttagaaataa gtcggctatt tctctagttt
     6661 tctagtaatg acaaattgtc gataaccata gtcatatcaa gggtttaagc tattcgactt
     6721 attgctaata aggcttaaaa agccttccaa aaattccact agaaatgagg tgagaaattg
     6781 aatcatgagg aatttataaa ttcaactgaa atagatgata aatttttaaa gcaaatcatt
     6841 gataataggg aatttaatga cgttatagtc tcgtattatg aacgactaca taaagaatca
     6901 cgcgatgatg gaatgaagaa gaagcaggaa aacgttaaag gttgcaatga tttttggatt
     6961 atagacaaat atcatgaaca tagattaaaa gagtttaaac gaactaatct ttgcaaggac
     7021 aagttttgta acaactgtaa aaaggttaaa caagcttcac gcatgggtaa attcatacca
     7081 cttattcagc cttacgcgga aaatatgtat caattaacgc taactattcc taatgttaaa
     7141 ggcgatgaat tgaaagatac actaagcaag atgcaaaaag cttttgctcg tttaattgag
     7201 tatttcaaag gcaaaaagaa aataaacgga attgattttg aagttttaca atacgaaggc
     7261 gctatacgat ctttagaaat aacatttaac ggtaatgagt atcatccaca ttatcatgct
     7321 cttattgttt taaaggttaa taaattccat gattgcatga taagaaaaca taaaaatgtt
     7381 tattcaatag acttcactgg aaaacgtgag gcgcgtttat ttagtgatat agaggttttg
     7441 atacagaagg tttggtactt gattataaac ggtaaacgga taacagaaac agcgatacag
     7501 tcacttaaaa aaggttattc atgtcagttg gacaagttta gcgaaaacga ttttgtagag
     7561 atatttaagt acatgacaaa agcaacaacg gaagacggta aaacattgag ttataagcaa
     7621 ttcaaaacgt tgtattatgc gttatttaaa gttagacaaa tacaagggta tggcatcttt
     7681 tataatttga aggatgatga agaaattaat tttgatgaag ttaatgaagc gtatgcggaa
     7741 ttcatagagg aattacagac aaaagaaaaa cctgttacgg tatcagaagc accagaacag
     7801 gtcagaaaag aaagtgacta tacagtgttt agtcgaaaaa cgttccaaaa atattggaag
     7861 caagtcatga gcgaatagcc ttactcattc cggcaagttt gataaaaggc tatctttacc
     7921 accattaatc aaataacgga gggttttcta atatcattat acataatttt aattcgtatg
     7981 atattaggaa attccttcta tatcgttaat tagtagagac tatgaattat ttcgtagtct
     8041 ttttttattg gaaaggaatg attgttgtta tatttagagg tttataacag gaaaaaggcg
     8101 cctgatctcg tttgaatcag aacgaaatca agcgcctttg ggcgataatt aaccaagaac
     8161 aagttaatta aataaatcta aaagtttttg gaagtactta tattatatcg tatatggagt
     8221 tcccggacag taattttagc ctaaatttgc gtgaagcaaa tcattaatca taccacgtaa
     8281 actatatatt cgtaaatagg taaatgttaa aataagagt
//
