LOCUS       NZ_AP022969             3940 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain J62 plasmid pJ62_5, complete sequence.
ACCESSION   NZ_AP022969
VERSION     NZ_AP022969.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203336
            Sequence Read Archive: DRR206376, DRR206400
            Assembly: GCF_021655215.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 428x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655215.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 07:12:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,996
            CDSs (total)                      :: 5,850
            Genes (coding)                    :: 5,586
            CDSs (with protein)               :: 5,586
            Genes (RNA)                       :: 146
            rRNAs                             :: 12, 12, 12 (5S, 16S, 23S)
            complete rRNAs                    :: 12, 12, 12 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 264
            CDSs (without protein)            :: 264
            Pseudo Genes (ambiguous residues) :: 0 of 264
            Pseudo Genes (frameshifted)       :: 134 of 264
            Pseudo Genes (incomplete)         :: 142 of 264
            Pseudo Genes (internal stop)      :: 40 of 264
            Pseudo Genes (multiple problems)  :: 44 of 264
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022969.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="J62"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pJ62_5"
                     /geo_loc_name="Japan:Tochigi"
                     /collection_date="2006"
     gene            836..1237
                     /locus_tag="L6484_RS29790"
                     /old_locus_tag="BCJMU62_p51"
     CDS             836..1237
                     /locus_tag="L6484_RS29790"
                     /old_locus_tag="BCJMU62_p51"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236801727.1"
                     /translation="MNKIAKEHLTIGEKPAREALKKAGCYATSGKKGWFFDGDAEILEK
                     SIYDFATVKATANPRTKKQISTKTKDVNNKGDEEPMNGTTNDVKNKRKRASFDIDVDLL
                     KQLKVKAVLEEKNLYDLVEQAIKDFLNKK"
                     /name="hypothetical protein"
     gene            1487..1753
                     /locus_tag="L6484_RS29795"
                     /old_locus_tag="BCJMU62_p52"
     CDS             1487..1753
                     /locus_tag="L6484_RS29795"
                     /old_locus_tag="BCJMU62_p52"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236801728.1"
                     /translation="MKFTINKKFILAKVEEKEKINLYTLVDNDNYEKITTIGVKPELKI
                     EERSLVEAEVSIRTQSERFELKNNEKKYVEVASFFVSKIQKVK"
                     /name="hypothetical protein"
     gene            1782..2018
                     /locus_tag="L6484_RS29800"
                     /old_locus_tag="BCJMU62_p53"
     CDS             1782..2018
                     /locus_tag="L6484_RS29800"
                     /old_locus_tag="BCJMU62_p53"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236801729.1"
                     /translation="MTRYPVFQEYQCTYPDPLLEFDIDWWTVSELKELFDITLTDKEKE
                     TRIQAFKTAQGYRGYAAVYSKKEILDLRLVINE"
                     /name="hypothetical protein"
     gene            2620..3309
                     /locus_tag="L6484_RS29805"
                     /old_locus_tag="BCJMU62_p54"
     CDS             2620..3309
                     /locus_tag="L6484_RS29805"
                     /old_locus_tag="BCJMU62_p54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002205120.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_236801726.1"
                     /translation="MSQLNEKRKKEREDAHNEKKAKFEALPNGVKAISKALNKASKKAH
                     NKDLEIEKSKQVLTDEEMEKACELQSKAKAQGMVLLPERRVKNRARFVQIMQDNWSFIQ
                     GHQYLTSEQIVFLMSLVPHVAIGSNAIVDVPRKKQPVGLNQSEIAAVLGTSKAKVSRVV
                     KSLVEKGVLAKAESGLEGNNVKSYVIFVNPHILISGSKDSVEDSLKIMFAKAMKMPVLK
                     NLPERLF"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 agcaataata aaaaacctaa aaaaatttaa tgttaagtta aattctcatg actcccaaca
       61 aggactatta tgagggtgaa gcaacaccct cataatagtc cttggtcttt tagaacttta
      121 acaacaacaa ttgcccttat ttctgattcg tcagaagtag gggtattttg ttcattcctt
      181 aaaacaattt tcgataatta tataaattaa agacaccaaa aatggtgtct ttttttgttt
      241 ttatcaaaat tagtaattgt ttatttgttt ttgataaaaa atagctataa accttgttat
      301 atcaatgttt ttcgtagtta tcaaaattgg taaccggtat ttttcgtagt tatcaaaatt
      361 ggtaactaga gttatcaaaa ttggtaacta cgaaaagtct caaaatcctt gatattattg
      421 aagattttat aaaaatacag tatgttccct tcttattcta tatatgtgat aaagctaaaa
      481 gttccgcaag gaacaggtag tatttttgaa aaaaccattc aaaaatctcc accttcctca
      541 ctttgttcgg tccttgcgga actttttttg gcacgtgcag tggctttacc aagccgatat
      601 acaaaaaaga aggaaattta tctatcaact tggactagca attatacgcg ccaaaccgcc
      661 atgctatcgc tcctaatagc acttacttga taattgtata cgtagtaata cgtggggtct
      721 ccccacaacc cgaccagcct caatcaaaaa agcaggggca aagccaaggc gaacgaaaaa
      781 atatttgaaa taaaagaact aaattgatat tcttgattta acaaagaaag aacctttgaa
      841 caaaatagcg aaagaacatt tgactattgg ggagaaaccc gctcgtgaag cgcttaaaaa
      901 ggctggttgt tatgcaactt caggcaaaaa aggttggttt tttgatggag atgcagagat
      961 tttagaaaaa agtatttatg attttgcaac ggtaaaagct acggcgaacc caagaactaa
     1021 aaaacaaata agcacaaaaa ctaaagatgt taacaataaa ggagatgaag aacctatgaa
     1081 cggaacaact aatgatgtaa agaacaaaag aaagagagcg tcttttgata tcgatgtgga
     1141 tttgctaaaa caattgaaag ttaaagcagt attggaagaa aagaacttat acgatttagt
     1201 ggaacaagct attaaagatt ttttaaacaa aaaatagatt catgtcaacg gtaccggacg
     1261 tgtccgacgg acaaaggagg ataagaagtt gatatggcac tacactaaaa atcctcttgt
     1321 tgtcttccaa acaatgtttg gaaggcgcca tccggcgagg agagggcccc ctttgggggt
     1381 ttgggggatt aaaaaagtgt aattaaaaaa ggtgttaata aagtttgaca taatgaaatt
     1441 ggaggttaca atacatgaaa ttttaaatat aaaaggggat gaaaatatga aatttacgat
     1501 taataaaaaa tttattttag ctaaggttga ggaaaaagaa aaaattaatt tatatacgtt
     1561 agtggacaat gacaattatg aaaaaattac aacgattggg gttaaacctg aattaaaaat
     1621 tgaagaacgt tcacttgttg aagctgaagt aagtattcgt acacaaagcg agcgttttga
     1681 attaaaaaat aacgagaaaa aatatgtgga agttgcgtca tttttcgtat ctaagattca
     1741 gaaagtgaag taaatctatg tatacaaaag aacaagtaga attgactagg tatccggtat
     1801 ttcaagaata tcaatgcacg tatccagatc ctttactaga atttgatatc gattggtgga
     1861 ccgtaagcga gcttaaagaa ctttttgata taacgcttac cgataaagag aaagaaacta
     1921 gaatacaagc atttaagacg gcgcaaggtt acagagggta cgcagctgtg tattctaaga
     1981 aagaaatatt ggatttgaga ttagttataa atgagtagtg tagcgcgtag cttgtcgtag
     2041 cctagttttt tattctttgt catttcgctt ttcgaaccaa gcacggaacg aacgcagcct
     2101 cgcgaataag tgaacttccg tccttggttc ggaagcgaag acaaagaaag tgaggtggat
     2161 ctagatgcta gaatggattg taaaaaaatt tgtagcatta atttcgtggt ttttctttat
     2221 aaaaatggga atcgaagatt tttatgaaaa ataaaattgt gattagctct agaatcgatt
     2281 ttaaggtacc tttgtgaaaa atctagggaa gacatgtagt ttctcgtcaa aaagcgtctt
     2341 agaatcgttt tgagaggtta aaagatatat atcgaattaa caagaagact atcgaattaa
     2401 taggaagcct ggtgaggctt gcagaaagaa tcttagattg ctaaaattca agagaaaaga
     2461 aaaagctctc aagcggtcac ttgagagctt tgtgtaaaca gtacaaatag tacttagtca
     2521 tgtaaaataa gtataatata ttatcatatt tttgtgtagt tcaaaaagtg tacgtttacc
     2581 ttcttttctc tttttttaaa acgagagggg aatacatata tgagtcagtt gaatgaaaaa
     2641 cgtaaaaaag agcgtgaaga cgctcataat gaaaaaaaag ctaagtttga agcacttcca
     2701 aatggtgtta aggcaattag taaagcattg aacaaagctt ctaaaaaagc tcataacaag
     2761 gatttagaaa tagaaaaaag caagcaagtt cttactgatg aagaaatgga aaaagcttgt
     2821 gagttacaaa gtaaagctaa ggcgcaaggg atggtattat tacctgaaag aagagttaaa
     2881 aatcgcgcac gatttgtaca gattatgcag gacaattgga gttttattca aggtcatcaa
     2941 tatttaacaa gtgaacaaat tgtcttttta atgagtttag ttcctcatgt agcaattgga
     3001 tctaacgcaa ttgttgatgt acctcggaaa aaacaacctg tgggtctcaa tcaaagtgaa
     3061 attgcagccg tattaggaac aagtaaagca aaggttagta gggttgtgaa gagccttgta
     3121 gaaaaaggtg ttctagctaa agcggaaagc ggtttagaag gcaataatgt taagtcttat
     3181 gtgatttttg taaatcctca tattttgatt tctggtagta aagacagtgt tgaagatagt
     3241 ttaaaaataa tgtttgccaa agctatgaaa atgcctgttt taaaaaattt accagagagg
     3301 ttattttaag gggtcccgcc agtattttgg aaaaaaacaa cgctaagagc atgcaagatt
     3361 ttatacagtt tttcggttaa ttgagtaaca aacgagaact cgaaaactca cattatgata
     3421 ggaatgtata aaaaatgtat agattgatac aaagaaaaaa gcgaatctct tatgtaagta
     3481 agggattcgc ctttttcttt gtttcccata atgagacgct atgtttgccc attaatcaaa
     3541 gctttttctt ttaagtttgt gcatcatagt gtaatataat ttttgttagg taaacaacct
     3601 catttcgagg cactgtaaag gggaatatat atatgtacgc agataaaaat atagtagagg
     3661 taggactggt atttaatgat gatgcaccta agattcatag agggtacatt aagtcggata
     3721 gacccctaga ggtgcaatta gaagaacgat tcatatctat tcaaatatac ggattatgga
     3781 aacatatttc tacacgacgg atcattgaaa tatgggaagc caaaacacaa agatatctat
     3841 aaaggtatct tttttgtcgt atacagtaga caaagttata acattgttat aacttaatat
     3901 tgtaaggaaa acaactaacg gtattgttta tcttacacgt
//
