LOCUS       NZ_AP022967             6561 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain J62 plasmid pJ62_3, complete sequence.
ACCESSION   NZ_AP022967
VERSION     NZ_AP022967.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203336
            Sequence Read Archive: DRR206376, DRR206400
            Assembly: GCF_021655215.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 428x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655215.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 07:12:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,996
            CDSs (total)                      :: 5,850
            Genes (coding)                    :: 5,586
            CDSs (with protein)               :: 5,586
            Genes (RNA)                       :: 146
            rRNAs                             :: 12, 12, 12 (5S, 16S, 23S)
            complete rRNAs                    :: 12, 12, 12 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 264
            CDSs (without protein)            :: 264
            Pseudo Genes (ambiguous residues) :: 0 of 264
            Pseudo Genes (frameshifted)       :: 134 of 264
            Pseudo Genes (incomplete)         :: 142 of 264
            Pseudo Genes (internal stop)      :: 40 of 264
            Pseudo Genes (multiple problems)  :: 44 of 264
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022967.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="J62"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pJ62_3"
                     /geo_loc_name="Japan:Tochigi"
                     /collection_date="2006"
     gene            complement(join(5984..6561,1..535))
                     /locus_tag="L6484_RS29725"
     CDS             complement(join(5984..6561,1..535))
                     /locus_tag="L6484_RS29725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002019875.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_000036645.1"
                     /translation="MSKITNDIDLKIDEVYEKVKDMKITFDENNTTKKQLTDSTINTYC
                     GIVKSYNNWLIQTHGISVDRAKPRHAMEYLETKIQAFEKGNASAFSMRQMAHAFHSFRV
                     ASAETGTFKHTCKVADKRDMLNTLTERGIFRKSADSTVLKANHSDYEKVQKELNVATTS
                     KHLREDIKTVHELQRYAGMRVHEAVKLKVSDIDFKSGTVYIKGKGGHERTVKIESKELL
                     EKLKNLCTGKSERSQVIIARKRDKNQLTNEEFGKRAKEEIRGAAVNAGVDRNGKRYTTH
                     SGRKAYAQAQMDKYKSYSKNQLKKEVAKRCQDKKTKQKYDAVIKNIRKKITEGSKNEGR
                     KLTNKEMCQFLVSLDLGHYRLDVVRYYADY"
                     /name="tyrosine-type recombinase/integrase"
     gene            1718..2281
                     /locus_tag="L6484_RS29730"
                     /old_locus_tag="BCJMU62_p31"
     CDS             1718..2281
                     /locus_tag="L6484_RS29730"
                     /old_locus_tag="BCJMU62_p31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002078386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_002019922.1"
                     /translation="MEVIMSISKHANKKKNILIAASEIVKEEGVVKLTLEAVAQRAGVS
                     KGGLLYHFPSKEALIKGMVEDWTNNYFECINTLVNNDDDNAIGKWNRAYLKSTFSDLEN
                     NNLNSALMAAMFINPDLLDEFRQRYDILHTKLITDGIDPVKITITRLSIDGLWFSEIFG
                     MAPLNEELKTQVFDELINMIQEDE"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            2281..2658
                     /gene="qacH"
                     /locus_tag="L6484_RS29735"
                     /old_locus_tag="BCJMU62_p32"
     ARG             2282..2655
                     /name="qacH"
                     /gene="qacH"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacH"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.2"
                     /coverage="100.0"
     gene            complement(3442..4062)
                     /locus_tag="L6484_RS29740"
                     /old_locus_tag="BCJMU62_p33"
     CDS             complement(3442..4062)
                     /locus_tag="L6484_RS29740"
                     /old_locus_tag="BCJMU62_p33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000963189.1"
                     /translation="MAIDFSQADRNARKRDLEKEKFFSDEEWELANDLQSRANARGMKL
                     VPERKIKNRAKFAQMLQDNVDYLEEIDYLDDKEIIFLWKISRKVGFLSNCLVDDIHKKS
                     PVPLTQTDIATLLKRTKNNVNPIVKSLVAKGILAVSKTGVQGNNSRANVYFMNPNILYA
                     GSKDDVNLTLQAMFSKVPKALKDMPVKLFELSCHSEEEEEKTN"
                     /name="hypothetical protein"
     gene            complement(4313..4750)
                     /locus_tag="L6484_RS29745"
                     /old_locus_tag="BCJMU62_p34"
     CDS             complement(4313..4750)
                     /locus_tag="L6484_RS29745"
                     /old_locus_tag="BCJMU62_p34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000094076.1"
                     /translation="MSSFRWYEENEVIGKTIIEEDDLTLLEAGKIYIDAIFCERIRFPV
                     QKNEQPIGFNFVYEYNVKKYFGLYDRSFMKIIKQEDLEEYHMRMIDFHVHRLSLGEELD
                     DCPIIGICYGSGPLITPRLEQVYDVSKTKAYRKYRLEKLFD"
                     /name="hypothetical protein"
     gene            complement(4764..5171)
                     /locus_tag="L6484_RS29750"
                     /old_locus_tag="BCJMU62_p35"
     CDS             complement(4764..5171)
                     /locus_tag="L6484_RS29750"
                     /old_locus_tag="BCJMU62_p35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000037919.1"
                     /translation="MSKKISIEEMTQEMKEKFTISLEDAHKILLDHGVTKSTNTQVTMR
                     WIRDGKIDALLIGKGRVETRKWLINKESLMEFVAFERLTLGDFKAMKEELEMLRAFKFE
                     VEKKPKRATGTRQKKDAATNTSKEKKEVPTK"
                     /name="hypothetical protein"
     ARG             2282..2658
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="61.68"
                     /coverage="116.82"
ORIGIN
        1 accgctgtaa ttcgtgtact gtttttatat cttctctcaa atgtttactt gtcgttgcta
       61 catttagttc tttttgaact ttttcatagt ctgaatgatt tgcctttaat accgtcgaat
      121 cagccgattt acggaagatt ccacgctctg tgagtgtatt taacatatct cgtttatcag
      181 cgaccttaca tgtatgttta aacgttcctg tttcagcact cgcaacacga aaagaatgaa
      241 acgcatgcgc catttggcgc atagaaaatg cggaagcatt tcctttttca aaggcttgaa
      301 tttttgtttc gagatattcc attgcgtggc gaggtttcgc cctatccacg ctaattccat
      361 gcgtttgtat taaccaattg ttatatgatt tcacgattcc gcaatatgtg tttattgttg
      421 aatcagttaa ttgttttttg gttgtattat tttcatcaaa agttattttc atatctttaa
      481 ctttttcata gacttcatca atttttaaat caatatcatt tgtaattttg ctcatttcgt
      541 caccccctat atacaaaatc aagagcaaga acccaacccc cgtgaacctt acaatatcaa
      601 ggcacaacaa aaacgaggtc tcatttgggt gggttcgatc tatttttttt aaagaccaaa
      661 aaaaatagac cgaaccaccc aaattaaaca cacaaaagca atcgcgaaat ctcccttcgg
      721 gcgatttaag ctcactagag tcaaagtcaa aatcaaaaga ccaaaagcaa aaatcaaggt
      781 caaaagatta aaagacaaag gctcaagata gctacgccat cttgttgcga ttggcgtcta
      841 taaatagact ccaaggctct cccatacatt ttgcaccaaa atgtatagtc aaatgagcgt
      901 acaacggtat tgcggtctgg aatttttgag taacccagaa attactgttc ttgcgatttg
      961 ttttgtggtt aaaaaccttt gttacagatt tacatttatt cagttgtaaa aatatttaat
     1021 tgtaaaactt gttcccttcg tattttttga tttgtcatct ccttaaattt ttatttttta
     1081 taatattttt ttccattctt gcaagcaaga attctatttt cttttaaagc tatttataaa
     1141 actacttggg aaagctttat attgctcctt tcttagatta ttttttccca cgtcttaaaa
     1201 acattttgct ttgctattgt aatgatcccc tttcattttt atgacgtgaa ttttttagtt
     1261 ttgtaaaata cacgattact tgaatcacgt cccaagattc atagtataaa atttcgtatt
     1321 tcatatttgc ttaaccgcaa gcgtttaaac aaataagtaa ttggttttaa aattactatg
     1381 aatatataca aattttacca ttaatcaact gattttgatg aaaaaacctt atttttttat
     1441 gcttttgaaa aggtcttacg accttaaatg tttttctttc cggggtagag tcctgaaaat
     1501 gcggatttac aacggtaatg taattttcac ccataaataa agccgatttt aagaaatcga
     1561 ctgttgaact aaagctccct ttactttaag tacatttctt cacaatcttt agattaattt
     1621 attggattat ttttatgtga aagagtgata ttaaataaga gctttaattc gattgacaaa
     1681 aaaaccgtcc agaaggtata ttatatgtat ctaagttatg gaggttatta tgtctatttc
     1741 aaagcatgct aataagaaaa aaaatatatt gattgctgca tctgaaattg taaaagaaga
     1801 aggagttgtc aaactcacat tagaggcagt cgcccaaaga gctggcgtga gtaaaggtgg
     1861 attattatac cattttccta gcaaggaagc attaattaaa ggaatggtgg aagattggac
     1921 aaataattat ttcgaatgca ttaatacgtt agttaataac gacgatgata acgcaatagg
     1981 aaaatggaat agggcttatc ttaaatctac attttccgat ttagaaaaca ataatctaaa
     2041 ttccgcacta atggctgcta tgtttattaa tccagattta ttagatgaat tccggcagag
     2101 atacgatatt ttgcatacaa agttaataac tgatggaatt gatcctgtca aaataacaat
     2161 tacaagatta tccatagatg gtctttggtt ttcagaaata tttggaatgg caccattaaa
     2221 tgaagaacta aaaacacaag tttttgatga actaataaac atgattcagg aggacgaata
     2281 atgtcatatc tatatttagc acttgcaata gtgggggaga ttattggctc ttccctatta
     2341 aaagcatctg aaggcttttc aaaactctat cctacaatag gggtaataat agctttcgtt
     2401 ggttgttttt tctttttatc cttgtcgcta aaaacaatcc ccttaaacac tgcatatgca
     2461 ttatgggcag gtttaggact tgttttgact acagtaattt cagttttagt ttggaaggaa
     2521 aaacttaaca tggctagcat tgcaggaata acactgattg ttgtaggagt tgtaatattg
     2581 aatttgtttg gaccaggaca tggagaatct ggtcatgaag caagcgaaac tattacaatg
     2641 aatgagacaa tggaataaca cataaaaaag tccttcttaa taaagagaag gactttttag
     2701 cttaattaag ggtatttttt gaagtcctta ttcaacaaac ctgcgtacgt tagtttaagt
     2761 gtatcacttc acaattccaa tagagtagag tttattgaga cataatgaaa ttagcgtttt
     2821 aaatgacttc tgactgtctc ataagacttg tatcaaatgc ttaccgttgt aaatccgcat
     2881 ttccctgacg ctaccccttt cctaagcaaa aatgttttta agtcttttat tcttttaccg
     2941 tattaaattt gatacggttt tcatatgctt ttttgggttg agctggctgg ggtttggggc
     3001 tagccccaag ggtttttatt acacaaaata ttgatagaac ggaaatgtac gcactaagcg
     3061 acggagcaat taaaatggat tgttcaagtg ataatccgaa aaagtgataa cttttttggt
     3121 ttatcgcttg gcacaagcca tgccaattgc ggagaatttt gagtcaagat aaagagtgga
     3181 gattttttgc ggtttttgca aaaaatacta ctcgtttctt tactcaaaat tagcgttaat
     3241 agagagtgag atagaacaac attgttaatg tattaaaaag tcaataatat caatggtttg
     3301 ttgcactttt cgtagttctt gaatttaaga accatagttc ttgaatttaa gaacaccgga
     3361 ttttttacat tttttacatt attttacata attgaaagat aaaaaaatac atgtattaaa
     3421 aaatacatgt attaattatt attaatttgt tttctcttct tcttcttctg aatgacatga
     3481 tagttcgaat aattttacag gcatatcctt taatgcttta ggaacttttg aaaacatcgc
     3541 ttgtaatgtt aaattgacat catctttgct accggcgtac aaaatgttcg gattcataaa
     3601 atacacattt gctctcgaat tattaccttg aacacctgtt tttgaaacag ccagtatgcc
     3661 ttttgctact aaagatttaa caataggatt gacattattt ttagttcttt tcaacaaagt
     3721 tgcgatatct gtttgtgtta aaggaactgg acttttttta tgaatatcat ccacaagaca
     3781 atttgataaa aaaccaactt tacgactgat tttccataaa aatataatct ctttatcatc
     3841 taaataatca atctcttcga gataatcaac gttatcctga agcatttgag caaattttgc
     3901 tcgattcttg attttacgct ctggaacaag tttcattcct cgcgcgttag ctcttgattg
     3961 caaatcattt gctaattccc attcttcatc tgaaaaaaac ttttcttttt ctaagtctct
     4021 ttttctagcg tttcgatccg cttgactaaa atcaattgcc attgattttg ctcctctcct
     4081 gaagaaaatg caacaagcat tctaggagat tcaccgttat ttttttatta tccgtaaagg
     4141 gttttaccct tgatttttaa atgaaaattc attaaaatta aaactagaaa tcaacgtttt
     4201 ggtgataaat tacgatgaat cacccaaagt cgagtgctcc aacactcggc ttttttttat
     4261 gcttgtctat tcaattctag cttaaaaact caaattttgt aagatgcttt acctaatcga
     4321 ataatttttc taatcgatac tttctgtatg cttttgtttt cgaaacatca taaacttgtt
     4381 ccaacctcgg tgttatcaaa ggtccacttc cataacaaat accaatgatt ggacaatcat
     4441 ctaattcttc ccctaaagat aatctgtgca catgaaaatc tatcattctc atgtgatatt
     4501 cttctaaatc ttcttgttta attatcttca tgaaacttct gtcatataat ccgaaatatt
     4561 tttttacatt atattcataa acaaaattaa atccaattgg ctgttcgttt ttttgaactg
     4621 gaaaccttat tctctcacaa aatattgcat caatatatat tttcccagct tctagcaaag
     4681 tcaaatcatc ttcttctatt atagtttttc caatcacttc attttcttca taccatctaa
     4741 aagatgacat gcaattcacc tctttatttt gttggcactt ctttcttttc ttttgaagta
     4801 tttgttgctg catctttctt ttgacgtgta ccagtagcac gctttggctt tttctctact
     4861 tcgaatttaa aagcacgtaa catttctaat tcttctttca ttgctttgaa atcacctaac
     4921 gttaaacgct cgaatgcaac gaattccatt agactttcct tgttaataag ccattttcga
     4981 gtctccacac gaccttttcc aatcaataat gcatcaattt tcccatcacg aatccaacgc
     5041 attgtaactt gtgtattcgt acttttcgtt acgccatgat ccaaaaggat tttatgtgca
     5101 tcttccagtg aaatcgtgaa tttttccttc atttcttgcg tcatttcttc aattgagatt
     5161 ttttttgaca ttgttttttc ctcctaatta caattaagta catgtatttt taattaaatg
     5221 tatccatcta tatttttaat atacagtaaa tgtattgaaa agtaaatata attttaaata
     5281 aatttattaa aaaatatatt taatctattt tacatttact attaattata tttactcaaa
     5341 attatatata tccttcttta aatttattat acattaaatt tatttttatg taaatccatt
     5401 ttaggtcatt acttttactt ttgtgtaaat ctatttattg ccatgacttt actattttgt
     5461 acatgtattt tatatctctt aaacgttgtt aaatcaacga ttttttgcga ttactaaaaa
     5521 taaattgcgg agacaataca tgtattttta tctatatttt tacttttgtg taaatctaat
     5581 ttaggtatat aaatttacta ttgtgtaaaa ctattaaaat tactttaaaa tacttttact
     5641 caaaaataaa tttactatac aataaatgta ttttaaaata cttttactgt atagtaaatt
     5701 taataggcgt ttcatttttt tttgattttc gaaaaaaagc gagaaatggc gaggatttgg
     5761 aaaactctcg tgtatcttct tagttcatcg acccgttttt tggcgatttc ctcgaatccg
     5821 cacaaggtac tggcattgcc atgagaggct aacacaaggc tttctcattg taaagccggg
     5881 aaagatggct tgacaacgag gctctagccc ttgtgtttct aagccagtgc gccagtacca
     5941 actgtgctcc gcactcgtct tcccgatttt ccgtcaaaac ctatcaataa tcagcataat
     6001 atcgtacaac atctaaacga tagtgaccca aatccaagct aactagaaac tggcacatct
     6061 ctttatttgt aagtttacgc ccttcgttct tcgaaccttc ggttattttt ttacggatat
     6121 tttttataac tgcatcatat ttttgctttg ttttcttgtc ctgacaacgt tttgcaactt
     6181 ctttttttaa ttgattctta ctgtaacttt tgtatttatc catttgcgct tgtgcatatg
     6241 ctttgcgacc tgaatgtgtt gtgtatcttt ttccgttacg atccacccca gcattgacgg
     6301 ctgcacctcg tatttcttct tttgcacgtt ttccaaattc ctcatttgtt aattgatttt
     6361 tatcacgttt tcgagcaata attacctggc ttctttcact ttttccagtg cataaatttt
     6421 tcaacttctc tagtaattct ttactttcaa ttttaaccgt tctctcgtgc ccgcctttgc
     6481 cttttatata gacagtgcca ctcttaaagt ctatatcact cacttttagt tttacggctt
     6541 cgtgaacacg cattccagcg t
//
