LOCUS       NZ_AP022963             5049 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain J51 plasmid pJ51_7, complete sequence.
ACCESSION   NZ_AP022963
VERSION     NZ_AP022963.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203335
            Sequence Read Archive: DRR206375, DRR206399
            Assembly: GCF_021655195.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 445x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655195.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 06:59:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,044
            CDSs (total)                      :: 5,892
            Genes (coding)                    :: 5,619
            CDSs (with protein)               :: 5,619
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 273
            CDSs (without protein)            :: 273
            Pseudo Genes (ambiguous residues) :: 0 of 273
            Pseudo Genes (frameshifted)       :: 143 of 273
            Pseudo Genes (incomplete)         :: 141 of 273
            Pseudo Genes (internal stop)      :: 51 of 273
            Pseudo Genes (multiple problems)  :: 56 of 273
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022963.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="J51"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pJ51_7"
                     /geo_loc_name="Japan:Tochigi"
                     /collection_date="2006"
     gene            complement(join(4758..5049,1..431))
                     /locus_tag="L6487_RS30015"
     CDS             complement(join(4758..5049,1..431))
                     /locus_tag="L6487_RS30015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_236796021.1"
                     /translation="MKKAFAENYINPVVERFSELDTPNGGFYTSIPQAVQYCWFLDDKE
                     LSVLLALSTYADENGRCKVYQRTLARKVKIKSTTTLRTKLKSLVKKRFILQVNTDGESS
                     IYQIKDYSTNPYILLSEAIEKVEQVAKKKYGVSVSKIAETMDELIASKIDYMAMITKIE
                     ESFNGYDVALKSVQGYVNEALGLEMDISLQTKKPRRERGFKMSKVIKPKQPKRNREFII
                     SEGESEAPVKREGLRLRL"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(524..676)
                     /locus_tag="L6487_RS30020"
                     /old_locus_tag="BCJMU51_p71"
     CDS             complement(524..676)
                     /locus_tag="L6487_RS30020"
                     /old_locus_tag="BCJMU51_p71"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796023.1"
                     /translation="MPKVVSSKEKMQPKTFKLHPRTIEQIKELAEKKQMTQAEVIDKAL
                     KLLAR"
                     /name="hypothetical protein"
     gene            1545..1691
                     /locus_tag="L6487_RS30025"
                     /old_locus_tag="BCJMU51_p72"
     CDS             1545..1691
                     /locus_tag="L6487_RS30025"
                     /old_locus_tag="BCJMU51_p72"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796009.1"
                     /translation="MKKTKKQAKKQPKKQPKKQMKTAMKIVGITKDVVNFVGNLIRIYT
                     FFF"
                     /name="hypothetical protein"
     gene            complement(1715..1915)
                     /locus_tag="L6487_RS30030"
                     /old_locus_tag="BCJMU51_p73"
     CDS             complement(1715..1915)
                     /locus_tag="L6487_RS30030"
                     /old_locus_tag="BCJMU51_p73"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796011.1"
                     /translation="MDIEAKKEAFVTFLVEKVLFQTFAERTTSLEEKEVYEKFIKLYDQ
                     ELNRLNFELTIASVNRKRANL"
                     /name="hypothetical protein"
     gene            complement(2399..2638)
                     /locus_tag="L6487_RS30035"
                     /old_locus_tag="BCJMU51_p74"
     CDS             complement(2399..2638)
                     /locus_tag="L6487_RS30035"
                     /old_locus_tag="BCJMU51_p74"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796012.1"
                     /translation="MEVKKIKIKPKKIVRNEENHKLEFDKVRLSIEIPISIRDNFKEAV
                     EKNGKTMKEVLEAYMQRYSVKELLTDLKEETIDN"
                     /name="hypothetical protein"
     gene            complement(2619..2957)
                     /locus_tag="L6487_RS30040"
                     /old_locus_tag="BCJMU51_p75"
     CDS             complement(2619..2957)
                     /locus_tag="L6487_RS30040"
                     /old_locus_tag="BCJMU51_p75"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="WP_236796014.1"
                     /translation="MSDNVNFPLRIPRELRDKFKKVSQKTGTSMNSSICQLIAEHIYKM
                     EQLGLKDIEEPNVIQVTPVKEETKAFIKKAIKEADEAKELAETKTKKRLRKSPKKKIGG
                     ETDGSKKD"
                     /name="Arc family DNA-binding protein"
     gene            complement(2969..3292)
                     /locus_tag="L6487_RS30045"
                     /old_locus_tag="BCJMU51_p76"
     CDS             complement(2969..3292)
                     /locus_tag="L6487_RS30045"
                     /old_locus_tag="BCJMU51_p76"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796016.1"
                     /translation="MMMNLLSLQQEANSVIQDLNGKDISSMSLIQKANYSNRIVKVRMS
                     LEAYRNTPGVNEFINALDHLRFKLAEVQQQETRQQANKRRAEIIQNGIKETPYPTMPMF
                     LNN"
                     /name="hypothetical protein"
     gene            complement(3710..4555)
                     /locus_tag="L6487_RS30050"
                     /old_locus_tag="BCJMU51_p77"
     CDS             complement(3710..4555)
                     /locus_tag="L6487_RS30050"
                     /old_locus_tag="BCJMU51_p77"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_236796018.1"
                     /translation="METDISIDRLTLVGYDTGMIEGYLKYSEYISFVGFSKNYGFRHSY
                     QGIKGELLEIGEKGKVRLDFNPNTANMEQVRDVVSRLKYIYLTRLDIAIDYFGVDLSEV
                     EWTSPKRRKQNLWLDPEGQQETRYIGAPSSERRYRIYNKKLERLEKKEELDPRAVNGQW
                     RVEVQQRFKESNNILDPIDYFTGDLFDIRPYRKVVDLSHIEKPMERIIIAGLMARPSEL
                     KNLSKNARGKYKKMIQEAREKSGVAINPEPIEVYTKEKDLLAEQLTSLFAHCAKSVAQV
                     "
                     /name="replication initiation factor domain-containing
                     protein"
ORIGIN
        1 gtttcagcta ttttagatac tgaaacacca tatttctttt tagctacctg ttcgactttt
       61 tcaatagcct cactaagaag aatataagga ttggtagaat aatctttaat ctgataaata
      121 ctagattctc catctgtatt aacctgcaat ataaaacgct tcttcactaa acttttcaat
      181 ttagttcgta atgttgttgt acttttaatc ttaactttcc tagctaacgt tcgttgatat
      241 accttgcaac gtccattttc gtctgcatag gtcgatagtg ctaataatac acttaactct
      301 ttatcatcta aaaaccaaca gtattgaact gcttgtggaa tggaagtata gaaaccgcca
      361 tttggagtat ctaattcact aaaacgttcc acaacaggat taatataatt ctctgcaaat
      421 gcctttttca ttacagatca ccttttcctt ttaattttcc ctccttgtta tcttgtttgc
      481 tatgttagta tgctacgtta tttgcgatgg gatatatatt tttttatcgt gctaataact
      541 ttaacgcctt atcaatcact tcagcttgcg tcatttgctt cttttcagct aattccttta
      601 tttgttcaat cgtcctagga tgcaatttaa atgttttggg ttgcatcttt tccttagaac
      661 taactacttt cggcataata tcactcctca acacaatgat aggtactccc taaaaacagc
      721 ttaacatcaa cgattctatt tgtccacttc aaaaacagtc aaaaaacaca taaaaaaata
      781 gaacaaacta ataaggacat ttttttgatc aaaaaagaaa ggagaaaacc taaaacaaaa
      841 aacattttga tatggtgaat ccctcgaaag aggacgctaa cccgctgtta gagaagcggg
      901 tttttcgacg tgtcgaacat ccctatagtt ataaaaaatc agagcaagaa ctagaataca
      961 tagttcttaa tcatccgaac aaaacccaaa ctttttggga gactattttc aaatgaaaaa
     1021 tcgttattgt tttgtataga aagagtaatg gcacttttga gctactattt gggctactaa
     1081 attagatacc tttttcaatc tttatccaag atacactttt ctatagagga taatctcgat
     1141 aaaaagacgg ataaatcacg gataaattaa ggataaagag aaggataaat actatgaaga
     1201 aaagctctaa aatttaacta agaaaagaat cacaccaaca cgaacaatat aatgtgccta
     1261 tctctttgaa gaaaaaaact cttaatacaa atctctcaaa aagtgtcaca aaacatagat
     1321 gaaaagacga atagaaaact aggaggtgtt attccattga caatccaaat tgatattttt
     1381 actcaagttt tatacatata gcaaaccaaa aaaccttagt atctgcgcct atgtttataa
     1441 aagaaatttt tttgaaaaag aataaaaaaa ataaggattt ctcaaaaaac gtcacaagta
     1501 atagatagca agacgaacaa ctactaaata gggggaatta atttatgaag aaaactaaaa
     1561 aacaagcaaa aaaacaacct aagaaacaac ctaagaaaca aatgaagaca gcaatgaaaa
     1621 tagtcggaat aacaaaagac gtggtcaatt ttgttggaaa tctaatccga atttatacat
     1681 tcttcttcta aacaaaaaga gctaatctat aacattatag gttggctctt tttctattta
     1741 cagatgctat tgtcaattcg aaatttagtc tatttaattc ttgatcatat agcttaataa
     1801 acttttcata cacctctttt tcttctaaag aagtagtcct ttctgcaaat gtttgaaata
     1861 gaactttctc tacaaggaag gtcacaaatg cttctttctt agcttctata tccataactt
     1921 acttcctcca ataagcggaa ttcaatcatt gacaacgagc ctccagtccg tgggtcataa
     1981 gccaatgccc cactccttca actcgctaac gctcaaacaa ggattggcaa gccataacag
     2041 gctaccacgg acattctcat tgtaaatgaa ccgctacgct ttgattcctt agtggtcttc
     2101 cctaatcttc gctaatactc gatgaaaacc aattaattta acccttaatt cttcgccaat
     2161 cctcgttaaa cctcgccatt tcttccctaa aaaccgacat accgtaaacc gcaaagacta
     2221 tgccctcaca tgaatgcctc ctggttcacc actatcttag gaagaatact tatagttgtt
     2281 tagcgtaaat ttagcgatat ttttattgat aggtcgtgtt ttttctactt ttaccaacta
     2341 taaaatttaa gccttaaaaa tgtacatgta ttcaaggttt aaacgaggta ttcagattct
     2401 aattatctat tgtttcttct tttaaatccg ttaatagctc tttaacacta tacctttgca
     2461 tatatgcttc taatacttcc ttcatcgtct tcccgttctt ttctaccgct tctttaaagt
     2521 tatctctaat agaaataggg atttcaatgc ttaatcttac cttatcaaat tctaatttat
     2581 gattttcttc attacgcact atttttttag gtttgatctt aatctttttt acttccatct
     2641 gtttcaccgc ctattttctt cttgggcgac tttcttaatc tttttttcgt cttagtttca
     2701 gctaattcct tagcttcatc agcttctttt atagccttct ttataaacgc ctttgtttct
     2761 tctttaaccg gcgtaacttg aattacatta ggttcttcta tatccttcaa tcctaattgc
     2821 tccattttat aaatgtgttc cgctattagt tggcaaattg atgagttcat actcgtccca
     2881 gtcttctgtg aaaccttttt aaacttatcc cgcaattcac gaggtattct caatggaaaa
     2941 ttaacattat cgctcatatc ctatcctcct aattatttag aaacattggc atcgttgggt
     3001 acggtgtttc ttttattcca ttttgaatta tttcagctct tcttttatta gcctgttgtc
     3061 gtgtttcctg ttgttgtact tcagctaact taaatcttaa gtggtccaat gcatttataa
     3121 attcatttac tcccggagta tttctatatg cctcaaggct cattctaact ttcactattc
     3181 gattagaata attagccttc tggatcaaac tcattgaaga aatatcctta ccgtttaaat
     3241 cttgaatcac actgttagct tcctgttgta aacttaacaa attcatcatc ataatatttt
     3301 ccctcttttc tgttgtgata atcaatacga ctattaaatt gattatcaat atgatagtca
     3361 tttataaaaa catcgtaact cttaaccaaa aatctgtata ctgggttact taaaaaattt
     3421 aaaatttatt tttaaattgt caccttacct atataaaaat taacaaaatc gagagggtgt
     3481 aggagcgcat tttcagatac agaacctaat aagcggtggg tggggtgttt ttcaaacaat
     3541 agcctaaata gacaactatt aatcctaaag cacgagctaa cgcaataaac ttaaaggaaa
     3601 tacatatatt cttaacgaat ccccttaaaa caaagaaaaa gagaggtgaa ttccctctct
     3661 tcttttgcta tactaacatt agatatttac ttgttataag ttttaaagtt tacacttgtg
     3721 ctacgctctt agcacagtgg gcgaacaggc ttgttagttg ctcagctaac aagtcttttt
     3781 cttttgtata aacttctatt ggttcaggat ttatcgctac accgcttttt tccctagcct
     3841 cttgtatcat cttcttatac ttacctctag cgttcttact aagattctta agttcactag
     3901 gtcttgccat taatcccgct attataattc gttccatagg cttttctata tgagataaat
     3961 caaccacctt tcgatatggt ctaatatcga atagatcacc tgtaaaataa tcaatagggt
     4021 ctaatatgtt attggactct ttaaaacgtt gttgtacctc aacacgccac tgtccattaa
     4081 ctgctctagg gtctaactct tctttctttt ctaaccgttc taacttcttg ttataaatac
     4141 gataccttct ctcacttgat ggcgcaccta tgtatctcgt ttcttgttgc ccttctgggt
     4201 ctaaccataa gttttgtttt cttcgtttag ggcttgtcca ctcaacctca gataaatcta
     4261 caccaaaata atctatcgct atatctaatc gcgttaaata gatgtatttc agtcgagaaa
     4321 cgacgtctct tacctgctcc atatttgcgg tattagggtt aaaatctaaa cgtaccttcc
     4381 ctttctcgcc tatctcaagc aattctcctt ttatcccttg gtatgaatga cgaaaaccat
     4441 aattcttaga aaaccccacg aatgaaatat attccgagta tttaagatac ccttctatca
     4501 ttccagtatc gtatccaacg agagttaggc ggtctatact aatatctgtt tccatcctaa
     4561 acactcattt ccgttttatt tgctatctta gtatgcttca gtaatttctt tgatagatat
     4621 atatattaat ttctttttag tattttatat ttagtagtaa tcaaattttg attaagaggt
     4681 acacaaaaat tgtgtaaggg gtaatcaatt tttgactagg tattcaagtc ctataaggta
     4741 atatataatt accttattca aagtcgtaat ctaagacctt cccgtttcac aggagcttca
     4801 gattcaccct ctgaaataat aaattctctg ttacgtttag gttgcttagg tttaataact
     4861 ttactcatct taaaacctcg ttcccgtcga ggttttttcg tttgtaaaga aatatccatt
     4921 tctaatccta aagcctcatt tacataacct tgtacagatt ttaaagctac atcataacca
     4981 ttaaaacttt cctctatctt agttatcata gccatataat ctatcttact agcaattaat
     5041 tcatccatc
//
