LOCUS       NZ_AP022962             5287 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain J51 plasmid pJ51_6, complete sequence.
ACCESSION   NZ_AP022962
VERSION     NZ_AP022962.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203335
            Sequence Read Archive: DRR206375, DRR206399
            Assembly: GCF_021655195.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 445x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655195.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 06:59:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,044
            CDSs (total)                      :: 5,892
            Genes (coding)                    :: 5,619
            CDSs (with protein)               :: 5,619
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 273
            CDSs (without protein)            :: 273
            Pseudo Genes (ambiguous residues) :: 0 of 273
            Pseudo Genes (frameshifted)       :: 143 of 273
            Pseudo Genes (incomplete)         :: 141 of 273
            Pseudo Genes (internal stop)      :: 51 of 273
            Pseudo Genes (multiple problems)  :: 56 of 273
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022962.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="J51"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pJ51_6"
                     /geo_loc_name="Japan:Tochigi"
                     /collection_date="2006"
     gene            complement(92..283)
                     /locus_tag="L6487_RS29980"
                     /old_locus_tag="BCJMU51_p61"
     CDS             complement(92..283)
                     /locus_tag="L6487_RS29980"
                     /old_locus_tag="BCJMU51_p61"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_076868343.1"
                     /translation="MQKAKRGRPRMNKTYRQNVVRFSYMEDTIKNIMDLVDSEERDKIN
                     ALLSNLVDHEKQKVAMKS"
                     /name="hypothetical protein"
     gene            complement(776..1117)
                     /locus_tag="L6487_RS29985"
                     /old_locus_tag="BCJMU51_p62"
     CDS             complement(776..1117)
                     /locus_tag="L6487_RS29985"
                     /old_locus_tag="BCJMU51_p62"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2538 family protein"
                     /protein_id="WP_080501341.1"
                     /translation="MYFENATHEQNYNDMMKFYSLAFGENPAFESAIYIAAYSQIHDKI
                     SKSKMDKNANPLAIALESDYLSNSAESLALLGQSLFNGEKVDVSSVVTRGKEIFEIFVQ
                     ACKIRGSLN"
                     /name="DUF2538 family protein"
     gene            complement(1136..1489)
                     /locus_tag="L6487_RS29990"
                     /old_locus_tag="BCJMU51_p63"
     CDS             complement(1136..1489)
                     /locus_tag="L6487_RS29990"
                     /old_locus_tag="BCJMU51_p63"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_076868344.1"
                     /translation="MASKNKHKIIEENRDIFLKRTSGMAHDLESVSNILVNFGLAKNRR
                     IAQRWVRENRIKAEWLSGEDDRRAGKIVKESEIFKTITTEIPALKVMFNAIEELQKEND
                     ELKKQIEQSSKQK"
                     /name="hypothetical protein"
     gene            complement(1503..1658)
                     /locus_tag="L6487_RS29995"
                     /old_locus_tag="BCJMU51_p64"
     CDS             complement(1503..1658)
                     /locus_tag="L6487_RS29995"
                     /old_locus_tag="BCJMU51_p64"
                     /inference="COORDINATES: protein motif:HMM:NF024513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3970 family protein"
                     /protein_id="WP_175556117.1"
                     /translation="MIKIRLQGLSQEMQEMIEDLKKHYDILSISDFYQNRNSQYIRCYI
                     EMRLKS"
                     /name="DUF3970 family protein"
     gene            complement(1896..2132)
                     /locus_tag="L6487_RS30000"
                     /old_locus_tag="BCJMU51_p65"
     CDS             complement(1896..2132)
                     /locus_tag="L6487_RS30000"
                     /old_locus_tag="BCJMU51_p65"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_076868345.1"
                     /translation="MKPNRYGRFRKEDVLKNGYEIVEYGITATEIEKQLGVKLTMKQRL
                     KHCIGFRCEKSWRGCVPVFDILDIALKNDRNQL"
                     /name="hypothetical protein"
     gene            complement(2164..2427)
                     /locus_tag="L6487_RS30005"
                     /old_locus_tag="BCJMU51_p66"
     CDS             complement(2164..2427)
                     /locus_tag="L6487_RS30005"
                     /old_locus_tag="BCJMU51_p66"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_220784068.1"
                     /translation="MKERYSVTQKYMVAGVDEKEKVSLYTLIEVPAIKKRMVVCIGNKV
                     DGLVVGNTYEVELDISIETKKFDTKDGVKYVDVASKFVRDIR"
                     /name="hypothetical protein"
     gene            complement(2663..5203)
                     /locus_tag="L6487_RS30010"
                     /old_locus_tag="BCJMU51_p67"
     CDS             complement(2663..5203)
                     /locus_tag="L6487_RS30010"
                     /old_locus_tag="BCJMU51_p67"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_220784067.1"
                     /translation="METHECGKQSVLVQDKATKEMYGFVAQCKRMDCDSCGTRIQTYVQ
                     NKIAMYTKKFDLKHFATITMGDSDIKNLLKKFGSLVDKTRELEKEYFIKRYISRKRSTR
                     EIAEKKYYEYVHSLYDQEHYYRWLYGKDLSKKEKNINYVPISNIGYQITKMITQRPARL
                     QDQIKIILEMKQGKKLRKNQLEKYYKDNVESQRKKWVGWSYADTKKEGRREEFLNDFHV
                     LLTDIAMNMYTQLYPNEEERTIYRSKIPKENTEMAYIRTIEFHNTVDAAHIHSLFNFYI
                     PIKLLKSVMGNHVYDMAEISNLASENLEEKCEEEHDFHQYVARYVSKYITKSSLEVYEK
                     LKEEGEDRVNIISSSDNISIVLEEAFETPYKGKFQLLSVVKGVLPQHYMKFSTIQEFQY
                     FILDSIADEHPNQYIDKTISCFKDYFKEARKKGLSNRDREILKDELKANENELLQNALK
                     LELERRSKEIFVPKQLKLRKTLKQGSLTTEQFEALQGITSSDLFSFLIGYAGTGKTYTL
                     STLLDYIDFEKHDVAVVSYTAQATQRVRSMLKEQNINVDNLEINTIHKLCGAKHSMIDY
                     PIFYNDWSKPLPYSFVIIDEFSLIDIRVLTSFLASLKPTTKVLFVGDPKQLNPIHSKHP
                     YFYLKDFAGVYELTKVLRSGDRIIDVATSVRNGDIEKVPFEVMDFKNSEEKIAEYKKRG
                     YQVLSNSVALKDWINNLFQKDKKQIQSKFNYAQFDNIIFIKNDQYKRYVNGEKGQIIDY
                     NEFGVTIRTENNNEVVLKDYEFFNRVEPNHCLTVHKSQGSEYDKVLVLLDATKTLLLNR
                     NLLYTAITRAKTDVVVMINKVDDKVKTLLKQEQE"
                     /name="AAA family ATPase"
ORIGIN
        1 aggctcatag ctgttttcta attgagaaag tgacttttgt gcgggtattt tcgccattac
       61 tgtaatcaag ttaagggaaa agtcgctcac ctcaactttt cattgcaact ttttgtttct
      121 catggtctac taaattactt aataaagcat tgatcttatc acgttcttct gaatctacta
      181 aatccataat gttttttatt gtatcttcca tgtacgagaa acgaactacg ttttgacgat
      241 atgttttatt cattcttggt cttcctcttt tggctttctg catctatcac acctcctttc
      301 tatttttttg ttatacaata aatagtttag cgcaaaattg caaaattcct agtttttttt
      361 gcaaaaattt ttactttcat agggaatccg cttgttcctc gtcaaatgat aggtagtatt
      421 tttgcgaaga ccatgcaaaa atctccacct aaacatcatt tgacaagtac cattttggaa
      481 cgctttttgt catgcaccaa gccgacatac caaaaagctc actaatttgt tttcgtcttg
      541 tttgcctatt gtagcgtccc aaaaacgctc ggcaaaagca aaaccttgct gactcgtcag
      601 caaaccacga accagtacct ctacccaaaa agtctcgaaa acccttcttc tatgtatttt
      661 aacaaagttc aatgttaaac tttagacttt ttaattatac caattccctc aaagttcaac
      721 cttatacatt gtatatatta tattttagta aaaacactta gtttattact aagtgttaat
      781 tcaaactacc acggatttta caagcttgaa caaaaatttc aaagatttct ttacctcttg
      841 taacaacaga agaaacatct actttttccc cattgaaaag cgattgcccc aataatgcca
      901 atgattctgc tgaatttgat aagtaatcag attccaatgc aattgccaat ggattcgcat
      961 ttttgtccat tttactttta gaaattttat catgaatttg cgaataagct gcgatataaa
     1021 tcgcactctc aaatgctgga ttttcaccaa aagctagtga ataaaatttc atcatatcat
     1081 tataattttg ctcatgagtt gcattttcaa agtacataca aacaccccta ttttcttatt
     1141 tttgtttgct cgattgttcg atttgctttt ttaactcatc attttctttt tgtaattcct
     1201 caatggcatt gaacattacc ttcaacgctg gaatctcagt tgtaattgtt ttgaatatct
     1261 cactctcttt aacaattttc cctgctcggc gatcatcctc gccacttagc cattcggctt
     1321 taatacgatt ttctcgcacc caacgttgtg caatcctacg atttttagct agtccaaaat
     1381 tcactaaaat atttgaaact gattctaaat catgtgccat gccacttgtt ctttttaaga
     1441 aaatgtcacg attctcttca atgattttgt gcttattttt tgatgccata ataacaacct
     1501 ccctatgatt ttaaacgcat ttctatataa catcgtatgt attggctatt gcggttttgg
     1561 tagaaatcag aaatggataa tatgtcataa tgcttcttca agtcctcaat catttcctgc
     1621 atttcttgag ataaaccttg caaacgaatt ttgatcatat tcaattcctc ccataattcc
     1681 attttacaac aaagtcatac aaagttcaac cttaaacttt atattttatt tatatatgta
     1741 tcatgcaagg catgatggtt gtgagtggta cggtgtaggt gtgtcttttt agctgtatag
     1801 ctgtgcttcc caagcacggc acagtaatcc agtgacaccc cagcacccca cctctagcaa
     1861 cgctacgcaa atttaatggt aagcttacat gtgttttata attgatttct atcattcttc
     1921 aacgctatat ctagaatgtc aaagacaggc acacagccac gccatgattt ctcacaacgg
     1981 aatcctatac aatgtttcaa tcgttgcttc atggtcaatt ttacgcctaa ttgcttctca
     2041 atttccgttg ctgttatgcc atattccacg atttcataac cattcttcaa aacatcttcc
     2101 ttgcggaatc gcccataacg attaggtttc aattgaacca cctccaccat gaaaattcat
     2161 aaattagcga atatcacgaa caaacttact agcaacatct acatatttta ctccatcttt
     2221 tgtatcaaac ttctttgttt caattgaaat atctaactca acctcatatg tattccctac
     2281 tactaatcca tcaactttat taccaataca aactaccata cgtttcttaa ttgctggtac
     2341 ttcaatcaat gtataaagtg aaactttctc tttctcatca acacctgcaa ccatatactt
     2401 ttgtgtaact gaataacgtt ctttcatgtt ttaagcaccc ctttttaata taaatttgtt
     2461 aaattataaa tacatttttc ttcttgtcaa gtttttcttt ctttttcctt tatataacat
     2521 acaaagttga actttatacg ttttacattt atttatttta attttatttt attttatcct
     2581 ttttttattt tatatttttt tactcagaaa aagacaaggt ttcccttgcc ttttatagaa
     2641 attaatataa gataaaataa atttactctt gttcttgttt taacaatgtt tttaccttgt
     2701 catccacttt gtttatcatt acaacaacat cagtttttgc cctggtgatc gctgtataca
     2761 ataggtttct attgagtaat aatgttttgg ttgcatccag taataccaat actttgtcat
     2821 actctgaacc ttgtgattta tgaacagtta agcaatgatt tggctctact ctattaaaga
     2881 attcataatc ttttagaaca acctcattat tgttttccgt tctaattgtt acgccaaatt
     2941 cattgtaatc aataatttgc cctttttcac cattcacata acgtttatat tggtcatttt
     3001 tgataaatat gatattatca aattgagcat aattaaattt tgattgaatt tgttttttat
     3061 ctttttgaaa taaattgttt atccagtctt ttaacgctac ggagttcgat agaacttggt
     3121 agcctctttt tttatattca gcaatttttt cttcgctgtt tttaaaatcc atcacttcaa
     3181 atggaacttt ttctatatct ccattcctca cagaagttgc tacatcaatg attctgtcac
     3241 cgcttcgcaa tacttttgtt aattcataaa caccagcaaa atcctttaaa taaaagtatg
     3301 ggtgttttga atgtattgga ttgagttgct ttggatcacc tacaaacaat acttttgttg
     3361 ttggtttcaa tgaagccaaa aatgaagtta acactcttat atcaatcaat gagaactcat
     3421 caatgattac aaatgaatat ggcaatggtt tgctccagtc attataaaat attggataat
     3481 caatcattga atgttttgcc ccacagagtt tgtgtattgt gtttatttcc aaattatcaa
     3541 cattaatatt ttgttctttc aacatgcttc taactctttg tgttgcttgt gctgtatagc
     3601 ttacaacagc tacatcatgt ttttcaaagt ctatataatc caacaatgtt gatagtgtat
     3661 aagtttttcc tgttcctgca tatccaatca aaaatgaaaa taaatcactt gaagtaatcc
     3721 cttgtaaggc ttcaaattgt tctgttgtca atgaaccctg tttcaatgtt tttcgcaact
     3781 tcaattgttt tggtacaaaa atttctttac ttcttctttc aagttctaac ttcaaagcat
     3841 tttgtaacaa ttcattttca tttgccttca attcatcttt taatatttct ctatctctat
     3901 ttgataatcc ctttttcctt gcttctttga agtaatcttt aaaacaagaa attgttttat
     3961 caatatattg atttgggtgt tcatctgcta tgctatcaag aataaaatat tgaaattctt
     4021 gaatcgttga aaacttcata taatgttgtg gcaacacacc ttttacaaca ctcaacaatt
     4081 gaaatttccc tttatatgga gtttcgaaag cttcttccaa cacaattgaa atattatcac
     4141 tggaagatat aatgttcaca cggtcttcgc cttcttcttt caatttctca taaacttcta
     4201 aactagattt tgtaatgtac tttgatacat atctagcaac atattgatga aaatcatgtt
     4261 cttcttcaca tttctcttcc aaattttcac ttgctaaatt actaatttca gccatgtcat
     4321 acacatgatt tcccatgaca cttttcaata atttaattgg gatatagaaa ttaaaaagag
     4381 aatgaatgtg tgctgcgtca acagtattgt gaaattcaat cgttcgaatg tacgccattt
     4441 ctgtattttc ctttggaatt ttcgaacgat aaatcgttct ttcttcttca tttggataca
     4501 attgtgtgta catattcatt gcaatatcag taagcaaaac atgaaaatca ttcaaaaatt
     4561 cttcacgtct tccttctttt ttagtatctg cataactcca tccaacccac ttttttcttt
     4621 ggctttcaac attgtcttta taatattttt ctaattggtt ttttcggagt tttttgccct
     4681 gcttcatttc caaaattatt ttaatttggt cttgaagtct tgctggacgt tgcgttatca
     4741 tttttgtgat ttgataacca atgttactga ttggaacata atttatattt ttctcttttt
     4801 tactcaaatc ttttccatat aaccatcgat aataatgttc ttgatcataa agagaatgga
     4861 catattcata atactttttc tctgcaattt ctcttgttga gcgttttcga cttatataac
     4921 gtttgataaa atattctttt tctaattctc ttgttttatc aaccaaactt ccaaatttct
     4981 tcaaaagatt ttttatgtct gaatcaccca ttgtgattgt tgcaaaatgt ttcaaatcaa
     5041 attttttggt gtacattgca attttatttt gtacatatgt ttgtattctt gttccacaag
     5101 aatcacaatc cattctcttg cattgtgcaa caaaaccata catctccttt gttgctttat
     5161 cctgtaccaa aacactctgt ttcccacatt catgcgtttc catcgactct ccctcccttc
     5221 ttgttgtcta attgagaatg aaaaaaaacg tacatggctt ttttgccccg aaacccaacg
     5281 ggctgta
//
