LOCUS       NZ_AP022961             8216 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain J51 plasmid pJ51_5, complete sequence.
ACCESSION   NZ_AP022961
VERSION     NZ_AP022961.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203335
            Sequence Read Archive: DRR206375, DRR206399
            Assembly: GCF_021655195.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8216)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 445x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655195.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 06:59:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,044
            CDSs (total)                      :: 5,892
            Genes (coding)                    :: 5,619
            CDSs (with protein)               :: 5,619
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 273
            CDSs (without protein)            :: 273
            Pseudo Genes (ambiguous residues) :: 0 of 273
            Pseudo Genes (frameshifted)       :: 143 of 273
            Pseudo Genes (incomplete)         :: 141 of 273
            Pseudo Genes (internal stop)      :: 51 of 273
            Pseudo Genes (multiple problems)  :: 56 of 273
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022961.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="J51"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pJ51_5"
                     /geo_loc_name="Japan:Tochigi"
                     /collection_date="2006"
     gene            complement(join(7145..8216,1..356))
                     /locus_tag="L6487_RS29945"
                     /pseudo=""
     CDS             complement(join(7145..8216,1..356))
                     /locus_tag="L6487_RS29945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053955.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /name="IS4 family transposase"
     gene            complement(686..1063)
                     /gene="qacH"
                     /locus_tag="L6487_RS29950"
                     /old_locus_tag="BCJMU51_p52"
     ARG             complement(690..1063)
                     /name="qacH"
                     /gene="qacH"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacH"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(1063..1614)
                     /locus_tag="L6487_RS29955"
                     /old_locus_tag="BCJMU51_p53"
     CDS             complement(1063..1614)
                     /locus_tag="L6487_RS29955"
                     /old_locus_tag="BCJMU51_p53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_000028030.1"
                     /translation="MSISKHANKKKNILIAASEIVKEEGVVKLTLEAVAQRAGVSKGGL
                     LYHFPSKEALIKGMVEDWTNNYFECINTLVNNDDDNAIGKWNRAYLKSTFSDLENNNLN
                     SALMATMFINPDLLDEFRQRYDILHTKLITDGIDPVKITITRLSIDGLWFSEIFGMAPL
                     NEELKTQVFDELINMIQEDE"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            2807..3919
                     /locus_tag="L6487_RS29960"
                     /old_locus_tag="BCJMU51_p54"
     CDS             2807..3919
                     /locus_tag="L6487_RS29960"
                     /old_locus_tag="BCJMU51_p54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036645.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_236796815.1"
                     /translation="MSKITNDIDLKIDEVYEKVKDMKITFDENNTTKKQLTDSTINTYC
                     GIVKSYNNWLIQTHGISVDRAKPRHAMEYLETKIQAFEKGNASAFSMRQMAHAFHSFRV
                     ASAETGTFKHTCKVADKRDMLNTLTERGIFRKSADSTVLKANHSDYEKVQKELNVATTS
                     KHLREDIKTVHELQRYAGMRVHEAVKLKVSDIDFKSGTVYIKGKGGHERTVKIESKELL
                     EKLKNLCTGKSERSQVIIARKRDKNQLTNEEFGKRAKEEIRGAAVNAGVDRNGKRYTTH
                     SGRKAYAQAQMDKYKSYSKNQLKKEVAKRCQDKKTKQKYDAVIKNIRKNITEGSKNEGR
                     KLTNKEMCQFLVSLDLGHYRLDVVRYYADY"
                     /name="tyrosine-type recombinase/integrase"
     gene            4732..5139
                     /locus_tag="L6487_RS29965"
                     /old_locus_tag="BCJMU51_p55"
     CDS             4732..5139
                     /locus_tag="L6487_RS29965"
                     /old_locus_tag="BCJMU51_p55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000037919.1"
                     /translation="MSKKISIEEMTQEMKEKFTISLEDAHKILLDHGVTKSTNTQVTMR
                     WIRDGKIDALLIGKGRVETRKWLINKESLMEFVAFERLTLGDFKAMKEELEMLRAFKFE
                     VEKKPKRATGTRQKKDAATNTSKEKKEVPTK"
                     /name="hypothetical protein"
     gene            5153..5590
                     /locus_tag="L6487_RS29970"
                     /old_locus_tag="BCJMU51_p56"
     CDS             5153..5590
                     /locus_tag="L6487_RS29970"
                     /old_locus_tag="BCJMU51_p56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000094076.1"
                     /translation="MSSFRWYEENEVIGKTIIEEDDLTLLEAGKIYIDAIFCERIRFPV
                     QKNEQPIGFNFVYEYNVKKYFGLYDRSFMKIIKQEDLEEYHMRMIDFHVHRLSLGEELD
                     DCPIIGICYGSGPLITPRLEQVYDVSKTKAYRKYRLEKLFD"
                     /name="hypothetical protein"
     gene            5841..6461
                     /locus_tag="L6487_RS29975"
                     /old_locus_tag="BCJMU51_p57"
     CDS             5841..6461
                     /locus_tag="L6487_RS29975"
                     /old_locus_tag="BCJMU51_p57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000963189.1"
                     /translation="MAIDFSQADRNARKRDLEKEKFFSDEEWELANDLQSRANARGMKL
                     VPERKIKNRAKFAQMLQDNVDYLEEIDYLDDKEIIFLWKISRKVGFLSNCLVDDIHKKS
                     PVPLTQTDIATLLKRTKNNVNPIVKSLVAKGILAVSKTGVQGNNSRANVYFMNPNILYA
                     GSKDDVNLTLQAMFSKVPKALKDMPVKLFELSCHSEEEEEKTN"
                     /name="hypothetical protein"
     ARG             complement(687..1063)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="60.75"
                     /coverage="116.82"
ORIGIN
        1 tgaaaatacg tgagcgccgc gcttgagtgg ccgatgtttc acagatttta cttttccata
       61 atgccgaaaa atatatttca aaaattcaac cgctttttat caaagcgttg tggagtcctt
      121 ctggactcat aagagtataa tgccgcatgt aattgactgc atagtcgaac aagcgagtca
      181 ctcgctgtac gttgacttac ccaaatacat atggtagcta attcatttcc agaaaacttt
      241 cgttttcttt ttacaaaacc taattctttt gcgagttctt ccaaaagaga aggggttaaa
      301 tgacgataca gttcttcaga aaatagttgt aattcatctt gaatcgaaag attcataaaa
      361 aatgtcaccc tttctagcta gctttttaga aagaataacg ttttttgtca cttgggggta
      421 gtgaaaattc ttaagttgat gggcatgggg tagcgtcagg gaaatgcgga tttacaacgg
      481 taagcatttg atacaagtct tatgagacag tcagaagtca tttaaaacgc taatttcatt
      541 atgtctcaat aaactctact ctattggaat tgtgaagtga tacacttaaa ctaacgtacg
      601 caggtttgtt gaataaggac ttcaaaaaat acccttaatt aagctaaaaa gtccttctct
      661 ttattaagaa ggactttttt atgtgttatt ccattgtctc attcattgta atagtttcgc
      721 ttgcttcatg accagattct ccatgtcctg gtccaaacaa attcaatatt acaactccta
      781 caagaatcag tgttattcct gcaatgctag ccatgttaag tttttccttc caaactaaaa
      841 ctgaaattac tgtagtcaaa acaagtccta aacctgccca taatgcatat gcagtgttta
      901 aggggattgt ttttagcgac aaggataaaa agaaaaaaca accaacgaaa gctattatta
      961 cccctattgt aggatagagt tttgaaaagc cttcagatgc ttttaatagg gaagagccaa
     1021 taatctcccc cactattgca agtgctaaat atagatatga cattattcgt cctcctgaat
     1081 catgtttatt agttcatcaa aaacttgtgt ttttagttct tcatttaatg gtgccattcc
     1141 aaatatttct gaaaaccaaa gaccatctat ggataatctt gtaattgtta ttttgacagg
     1201 atcaattcca tcagttatta actttgtatg caaaatatcg tatctctgcc ggaattcatc
     1261 taataaatct ggattaataa acatagtagc cattagtgcg gaatttagat tattgttttc
     1321 taaatcggaa aatgtagatt taagataagc cctattccat tttcctattg cgttatcatc
     1381 gtcgttatta actaacgtat taatgcattc gaaataatta tttgtccaat cttccaccat
     1441 tcctttaatt aatgcttcct tgctaggaaa atggtataat aatccacctt tactcacgcc
     1501 agctctttgg gcgactgcct ctaatgtgag tttgacaact ccttcttctt ttacaatttc
     1561 agatgcagca atcaatatat tttttttctt attagcatgc tttgaaatag acataataac
     1621 ctccataact tagatacata taatataccg tctggacggt ttttttgtca atcgaattaa
     1681 agctcttatt taatatcact ctttcacata aaaataatcc aataaattaa tctaaagatt
     1741 gtgaagaaat gtacttaaag taaagggagc tttagttcaa cagtcgattt cttaaaatcg
     1801 gctttattta tgggtgaaaa ttacattacc gttgtaaatc cgcattttca ggactctacc
     1861 ccggaaagaa aaacatttaa ggtcgtaaga ccttttcaaa agcataaaaa aataaggttt
     1921 tttcatcaaa atcagttgat taatggtaaa atttgtatat attcatagta attttaaaac
     1981 caattactta tttgtttaaa cgcttgcggt taagcaaata tgaaatacga aattttatac
     2041 tatgaatctt gggacgtgat tcaagtaatc gtgtatttta caaaactaaa aaattcacgt
     2101 cataaaaatg aaaggggatc attacaatag caaagcaaaa tgtttttaag acgtgggaaa
     2161 aaataatcta agaaaggagc aatataaagc tttcccaagt agttttataa atagctttaa
     2221 aagaaaatag aattcttgct tgcaagaatg gaaaaaatat tataaaaata aaaatttaag
     2281 gagatgacaa atcaaaaaat acgaagggaa caagttttac aattaaatat ttttacaact
     2341 gaataaatgt aaatctgtaa caaaggtttt taaccacaaa acaaatcgca agaacagtaa
     2401 tttctgggtt actcaaaaat tccagaccgc aataccgttg tacgctcatt tgactataca
     2461 ttttggtgca aaatgtatgg gagagccttg gagtctattt atagacgcca atcgcaacaa
     2521 gatggcgtag ctatcttgag cctttgtctt ttaatctttt gaccttgatt tttgcttttg
     2581 gtcttttgat tttgactttg actctagtga gcttaaatcg cccgaaggga gatttcgcga
     2641 ttgcttttgt gtgtttaatt tgggtggttc ggtctatttt ttttggtctt taaaaaaaat
     2701 agatcgaacc cacccaaatg agacctcgtt tttgttgtgc cttgatattg taaggttcac
     2761 gggggttggg ttcttgctct tgattttgta tatagggggt gacgaaatga gcaaaattac
     2821 aaatgatatt gatttaaaaa ttgatgaagt ctatgaaaaa gttaaagata tgaaaataac
     2881 ttttgatgaa aataatacaa ccaaaaaaca attaactgat tcaacaataa acacatattg
     2941 cggaatcgtg aaatcatata acaattggtt aatacaaacg catggaatta gcgtggatag
     3001 ggcgaaacct cgccacgcaa tggaatatct cgaaacaaaa attcaagcct ttgaaaaagg
     3061 aaatgcttcc gcattttcta tgcgccaaat ggcgcatgcg tttcattctt ttcgtgttgc
     3121 gagtgctgaa acaggaacgt ttaaacatac atgtaaggtc gctgataaac gagatatgtt
     3181 aaatacactc acagagcgtg gaatcttccg taaatcggct gattcgacgg tattaaaggc
     3241 aaatcattca gactatgaaa aagttcaaaa agaactaaat gtagcaacga caagtaaaca
     3301 tttgagagaa gatataaaaa cagtacacga attacagcgg tacgctggaa tgcgtgttca
     3361 cgaagccgta aaactaaaag tgagtgatat agactttaag agtggcactg tctatataaa
     3421 aggcaaaggc gggcacgaga gaacggttaa aattgaaagt aaagaattac tagagaagtt
     3481 gaaaaattta tgcactggaa aaagtgaaag aagccaggta attattgctc gaaaacgtga
     3541 taaaaatcaa ttaacaaatg aggaatttgg aaaacgtgca aaagaagaaa tacgaggtgc
     3601 agccgtcaat gctggggtgg atcgtaacgg aaaaagatac acaacacatt caggtcgcaa
     3661 agcatatgca caagcgcaaa tggataaata caaaagttac agtaagaatc aattaaaaaa
     3721 agaagttgca aaacgttgtc aggacaagaa aacaaagcaa aaatatgatg cagttataaa
     3781 aaatatccgt aaaaacataa ccgaaggttc gaagaacgaa gggcgtaaac ttacaaataa
     3841 agagatgtgc cagtttctag ttagcttgga tttgggtcac tatcgtttag atgttgtacg
     3901 atattatgct gattattgat aggttttgac ggaaaatcgg gaagacgagt gcggagcaca
     3961 gttggtactg gcgcactggc ttagaaacac aagggctaga gcctcgttgt caagccatct
     4021 ttcccggctt tacaatgaga aagccttgtg ttagcctctc atggcaatgc cagtaccttg
     4081 tgcggattcg aggaaatcgc caaaaaacgg gtcgatgaac taagaagata cacgagagtt
     4141 ttccaaatcc tcgccatttc tcgctttttt tcgaaaatca aaaaaaaatg aaacgcctat
     4201 taaatttact atacagtaaa agtattttaa aatacattta ttgtatagta aatttatttt
     4261 tgagtaaaag tattttaaag taattttaat agttttacac aatagtaaat ttatatacct
     4321 aaattagatt tacacaaaag taaaaatata gataaaaata catgtattgt ctccgcaatt
     4381 tatttttagt aatcgcaaaa aatcgttgat ttaacaacgt ttaagagata taaaatacat
     4441 gtacaaaata gtaaagtcat ggcaataaat agatttacac aaaagtaaaa gtaatgacct
     4501 aaaatggatt tacataaaaa taaatttaat gtataataaa tttaaagaag gatatatata
     4561 attttgagta aatataatta atagtaaatg taaaatagat taaatatatt ttttaataaa
     4621 tttatttaaa attatattta cttttcaata catttactgt atattaaaaa tatagatgga
     4681 tacatttaat taaaaataca tgtacttaat tgtaattagg aggaaaaaac aatgtcaaaa
     4741 aaaatctcaa ttgaagaaat gacgcaagaa atgaaggaaa aattcacgat ttcactggaa
     4801 gatgcacata aaatcctttt ggatcatggc gtaacgaaaa gtacgaatac acaagttaca
     4861 atgcgttgga ttcgtgatgg gaaaattgat gcattattga ttggaaaagg tcgtgtggag
     4921 actcgaaaat ggcttattaa caaggaaagt ctaatggaat tcgttgcatt cgagcgttta
     4981 acgttaggtg atttcaaagc aatgaaagaa gaattagaaa tgttacgtgc ttttaaattc
     5041 gaagtagaga aaaagccaaa gcgtgctact ggtacacgtc aaaagaaaga tgcagcaaca
     5101 aatacttcaa aagaaaagaa agaagtgcca acaaaataaa gaggtgaatt gcatgtcatc
     5161 ttttagatgg tatgaagaaa atgaagtgat tggaaaaact ataatagaag aagatgattt
     5221 gactttgcta gaagctggga aaatatatat tgatgcaata ttttgtgaga gaataaggtt
     5281 tccagttcaa aaaaacgaac agccaattgg atttaatttt gtttatgaat ataatgtaaa
     5341 aaaatatttc ggattatatg acagaagttt catgaagata attaaacaag aagatttaga
     5401 agaatatcac atgagaatga tagattttca tgtgcacaga ttatctttag gggaagaatt
     5461 agatgattgt ccaatcattg gtatttgtta tggaagtgga cctttgataa caccgaggtt
     5521 ggaacaagtt tatgatgttt cgaaaacaaa agcatacaga aagtatcgat tagaaaaatt
     5581 attcgattag gtaaagcatc ttacaaaatt tgagttttta agctagaatt gaatagacaa
     5641 gcataaaaaa aagccgagtg ttggagcact cgactttggg tgattcatcg taatttatca
     5701 ccaaaacgtt gatttctagt tttaatttta atgaattttc atttaaaaat caagggtaaa
     5761 accctttacg gataataaaa aaataacggt gaatctccta gaatgcttgt tgcattttct
     5821 tcaggagagg agcaaaatca atggcaattg attttagtca agcggatcga aacgctagaa
     5881 aaagagactt agaaaaagaa aagttttttt cagatgaaga atgggaatta gcaaatgatt
     5941 tgcaatcaag agctaacgcg cgaggaatga aacttgttcc agagcgtaaa atcaagaatc
     6001 gagcaaaatt tgctcaaatg cttcaggata acgttgatta tctcgaagag attgattatt
     6061 tagatgataa agagattata tttttatgga aaatcagtcg taaagttggt tttttatcaa
     6121 attgtcttgt ggatgatatt cataaaaaaa gtccagttcc tttaacacaa acagatatcg
     6181 caactttgtt gaaaagaact aaaaataatg tcaatcctat tgttaaatct ttagtagcaa
     6241 aaggcatact ggctgtttca aaaacaggtg ttcaaggtaa taattcgaga gcaaatgtgt
     6301 attttatgaa tccgaacatt ttgtacgccg gtagcaaaga tgatgtcaat ttaacattac
     6361 aagcgatgtt ttcaaaagtt cctaaagcat taaaggatat gcctgtaaaa ttattcgaac
     6421 tatcatgtca ttcagaagaa gaagaagaga aaacaaatta ataataatta atacatgtat
     6481 ttttaataca tgtatttttt tatctttcaa ttatgtaaaa taatgtaaaa aatgtaaaaa
     6541 atccggtgtt cttaaattca agaactatgg ttcttaaatt caagaactac gaaaagtgca
     6601 acaaaccatt gatattattg actttttaat acattaacaa tgttgttcta tctcactctc
     6661 tattaacgct aattttgagt aaagaaacga gtagtatttt ttgcaaaaac cgcaaaaaat
     6721 ctccactctt tatcttgact caaaattctc cgcaattggc atggcttgtg ccaagcgata
     6781 aaccaaaaaa gttatcactt tttcggatta tcacttgaac aatccatttt aattgctccg
     6841 tcgcttagtg cgtacatttc cgttctatca atattttgtg taataaaaac ccttggggct
     6901 agccccaaac cccagccagc tcaacccaaa aaagcatatg aaaaccgtat caaatttaat
     6961 acggtaaaag aataaaagac ttaaaaacat ttttgcttag gaaaggggta gcgtcacatg
     7021 cccatcaact taagaacgaa aacaaaatga actttcgttc aaacaaacta aaaaacacgt
     7081 aatgtacttt tggtattgaa aagtacacac accaaataaa ctctaacgag tttcatttta
     7141 aaaattatgc agctttcttt tgttttccaa acccattata ctcatagaca acacccataa
     7201 tatcaaagat agttttcttc tcatatcgat gagatttccg tccattcttc tgtaggaggt
     7261 cgaacaaacg gattaaaatc tttgttagat tttgggtgtt tctctgtatc gcttgatata
     7321 acagcgatac atgatcttga atcatcccaa tggctttata ttcacttaat tctcttttgt
     7381 tcttttgcaa aagaagttta cgcatcttaa acatcgtaga agaacagata aagatggcaa
     7441 taagtcttcc atacacatga cattctaatc gctctttttt gatagtttgc caatgatgaa
     7501 tttgaaataa ggatttccac gttttaaaaa taatttctat ttgccagcga agggagtaaa
     7561 agtcatgtat ttgttccatc ggaacaactc cttcaggcgt atttgagaca tatatgttga
     7621 tacccgttaa tcgtttactc ttttctgaaa atgtaatacc ctttttactt tcagtgtaac
     7681 tttgtttttt tctacgttcc tgtatctgtt tgtctgttaa acgatagata attacacgtg
     7741 tgaatagttt ttgattcttt ccaatgtaag cttcttttat ttcgtatgtt tgaccgggtt
     7801 ttaaattgtt cataatatgt tctaaatcaa cttggatata ctgtgattgc tttttacggt
     7861 tccgtttcga aagtattctg gaaacggatt tttacataca cggtatggtt taattttaat
     7921 ctggatatat aataagcacc tcgttgatcc atttgatcta agtcttctaa tgaaaaataa
     7981 cctaaatcac ggatacataa atcgcttgga cgtaatgtgt ctaagcattc tgttccaaag
     8041 gtcttatcat tatttttcct ggacctatct gaaagtttag aaactgtccg ctatgtaagt
     8101 catattctaa ttgaatcttg atacccgctg tttgtgcaca accacctgat ccaggatata
     8161 catgggctaa atgttttggt acttggaaaa tcgtcgcatc taaaatacga attcgt
//
