LOCUS       NZ_AP022960             8316 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain J51 plasmid pJ51_4, complete sequence.
ACCESSION   NZ_AP022960
VERSION     NZ_AP022960.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203335
            Sequence Read Archive: DRR206375, DRR206399
            Assembly: GCF_021655195.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8316)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 445x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655195.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 06:59:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,044
            CDSs (total)                      :: 5,892
            Genes (coding)                    :: 5,619
            CDSs (with protein)               :: 5,619
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 273
            CDSs (without protein)            :: 273
            Pseudo Genes (ambiguous residues) :: 0 of 273
            Pseudo Genes (frameshifted)       :: 143 of 273
            Pseudo Genes (incomplete)         :: 141 of 273
            Pseudo Genes (internal stop)      :: 51 of 273
            Pseudo Genes (multiple problems)  :: 56 of 273
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022960.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="J51"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pJ51_4"
                     /geo_loc_name="Japan:Tochigi"
                     /collection_date="2006"
     gene            join(8194..8316,1..477)
                     /locus_tag="L6487_RS29875"
     CDS             join(8194..8316,1..477)
                     /locus_tag="L6487_RS29875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562260.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_236788723.1"
                     /translation="MNKRFAYIRVSDKTQNENRQLDEMLQQEIEERDIFIDKASGKDFN
                     RPKYQALKQMLRAGDILYIKSIDRFGRNKDDILKEWNEITKEIGADIVVLDMPLLDTRQ
                     YKDVLGSFVSDLVLQILSFFAEEERRKIKERQREGIDAAQRRGAKFGRPAATFPPGFEE
                     VYKRWKDGEIKAIEAMEELELKRTTFYKLVNSFENK"
                     /name="recombinase family protein"
     gene            1230..1427
                     /locus_tag="L6487_RS29880"
                     /old_locus_tag="BCJMU51_p401"
     CDS             1230..1427
                     /locus_tag="L6487_RS29880"
                     /old_locus_tag="BCJMU51_p401"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788725.1"
                     /translation="MIGKDMITLDNPKKEYFQQYAQNNKEQINANARRRRQQQKEKVEK
                     YDRLVAALRSRGISVEELLK"
                     /name="hypothetical protein"
     gene            1850..2950
                     /locus_tag="L6487_RS29885"
                     /old_locus_tag="BCJMU51_p402"
     CDS             1850..2950
                     /locus_tag="L6487_RS29885"
                     /old_locus_tag="BCJMU51_p402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205124.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_098492605.1"
                     /translation="MNHEEFINSTEIDDKFLKQIIDNREFNDVIVSYYERLHKESRDDG
                     MKKKQENVKGCNDFWIIDKYHEHRLKEFKRTNLCKDKFCNNCKKVKQASRMGKFIPLIQ
                     PYAENMYQLTLTIPNVKGDELKDTLSKMQKAFARLIEYFKGKKKINGIDFEVLQYEGAI
                     RSLEITFNGNEYHPHYHALIVLKVNKFHDCMIRKHKNVYSIDFTGKREARLFSDIEVLI
                     QKVWYLIINGKRITETAIQSLKKGYSCQLDKFSENDFVEIFKYMTKATTEDGKTLSYKQ
                     FKTLYYALFKVRQIQGYGIFYNLKDDEEINFDEVNEAYAEFIEELQTKEKPVTVSEAPE
                     QVRKESDYTVFSRKTFQKYWKQVMSE"
                     /name="protein rep"
     gene            3418..3567
                     /locus_tag="L6487_RS29890"
                     /old_locus_tag="BCJMU51_p403"
     CDS             3418..3567
                     /locus_tag="L6487_RS29890"
                     /old_locus_tag="BCJMU51_p403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788727.1"
                     /translation="MEREKCSLKEAKNAYNRKWRAANRDKVKLYNDRYWEKKAKEMEEQ
                     ACQK"
                     /name="hypothetical protein"
     gene            3555..3827
                     /locus_tag="L6487_RS29895"
                     /old_locus_tag="BCJMU51_p404"
     CDS             3555..3827
                     /locus_tag="L6487_RS29895"
                     /old_locus_tag="BCJMU51_p404"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788729.1"
                     /translation="MSEVSMETVIKGKQQSELLKHLEKVGIELLGRRDELLEQWEKEGL
                     KEDSIFEDDLKFVEELMNRNDELMFDVKVELITTMDEIHHQKMGY"
                     /name="hypothetical protein"
     gene            3832..4083
                     /locus_tag="L6487_RS29900"
                     /old_locus_tag="BCJMU51_p405"
     CDS             3832..4083
                     /locus_tag="L6487_RS29900"
                     /old_locus_tag="BCJMU51_p405"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788731.1"
                     /translation="MLGKYDGLLEKERLLKRIDELSFALEASGDPMLEAMVKNSGNTDI
                     AVAKELITKGSELGMNEHVFKKALLAKVNELREEVTKL"
                     /name="hypothetical protein"
     gene            4080..4493
                     /locus_tag="L6487_RS29905"
                     /old_locus_tag="BCJMU51_p406"
     CDS             4080..4493
                     /locus_tag="L6487_RS29905"
                     /old_locus_tag="BCJMU51_p406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788733.1"
                     /translation="MKKDKQKDLPFNHKVVLSVLPLGRSKAITTKEIMARSGIKDKRAV
                     SAILSELVTVYKQPIATTSKKEFKGVFIVQDVIDYQIGKEGLVSRKRHIDKRDSAYTEA
                     CLEIFKGTVTLDESKNKVEGSVRACKQSASSSM"
                     /name="hypothetical protein"
     gene            4463..5170
                     /locus_tag="L6487_RS29910"
                     /old_locus_tag="BCJMU51_p407"
     CDS             4463..5170
                     /locus_tag="L6487_RS29910"
                     /old_locus_tag="BCJMU51_p407"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788735.1"
                     /translation="MQTISKFFNVDNESKLNELTIAKGELNSKESELQSKLSRVERALE
                     LAQTDLMIDDSTANKKVVAKYEEAQEKFQSELIATQEELNKVSDEIAAIVKEELHAELV
                     DAAKQDVMLIELPEKCRKAKKVINRDLYRATSFEDEGRFSRKYLHVSATVIDDARTFAD
                     IHRFADKPNLTRIGYQRGELIRWGGDNNLFKLNDEYETIYEEEAQKIKDKTDKEYEQFM
                     KAFNKYFAVSDSH"
                     /name="hypothetical protein"
     gene            5224..5649
                     /locus_tag="L6487_RS29915"
                     /old_locus_tag="BCJMU51_p408"
     CDS             5224..5649
                     /locus_tag="L6487_RS29915"
                     /old_locus_tag="BCJMU51_p408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788712.1"
                     /translation="MSKDTLKNIFHVYCFYTVRLQGDTEPRMRRNKLNYDNPIQNYYES
                     FIDCLRGFCEVRSDVSLYSFYQFVVDAVNSLNKQERKLIYERYLYKDHYKSDRQHYLAM
                     DITPQNYKKQMDPVRRKLIKNLGIEDLQLNIPDWMKR"
                     /name="hypothetical protein"
     gene            5780..6028
                     /locus_tag="L6487_RS29920"
                     /old_locus_tag="BCJMU51_p409"
     CDS             5780..6028
                     /locus_tag="L6487_RS29920"
                     /old_locus_tag="BCJMU51_p409"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_236788716.1"
                     /translation="MKSLYRILELDKDEVSSSEKLILLTIAIYSDNKFIPFSITELENY
                     TNLSRATVTRLVKQLEEKGFVEVQRNGTATNNYKVTL"
                     /name="helix-turn-helix domain-containing protein"
     gene            6105..6281
                     /locus_tag="L6487_RS29925"
                     /old_locus_tag="BCJMU51_p410"
     CDS             6105..6281
                     /locus_tag="L6487_RS29925"
                     /old_locus_tag="BCJMU51_p410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176543923.1"
                     /translation="MLPQKEQLGHLAEKTELVKDLAWIAHDLLTEEDYTKENAAEALIK
                     VINRELSYVSKVR"
                     /name="hypothetical protein"
     gene            6323..6529
                     /locus_tag="L6487_RS29930"
                     /old_locus_tag="BCJMU51_p411"
     CDS             6323..6529
                     /locus_tag="L6487_RS29930"
                     /old_locus_tag="BCJMU51_p411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788718.1"
                     /translation="MANTVAKDKFEIVEKFVNACMLKGVSEDKSFKLVEELCTLIQQKR
                     HDEWFGLVDEALKEAGHEEGVTL"
                     /name="hypothetical protein"
     gene            6526..6657
                     /locus_tag="L6487_RS30235"
                     /old_locus_tag="BCJMU51_p412"
     CDS             6526..6657
                     /locus_tag="L6487_RS30235"
                     /old_locus_tag="BCJMU51_p412"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272880692.1"
                     /translation="MSEVEMVLHEFEELLHKIISAAQHGDEQAMNHLDIVKAWLKDN"
                     /name="hypothetical protein"
     gene            6734..6973
                     /locus_tag="L6487_RS29935"
                     /old_locus_tag="BCJMU51_p413"
     CDS             6734..6973
                     /locus_tag="L6487_RS29935"
                     /old_locus_tag="BCJMU51_p413"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788720.1"
                     /translation="MAEEHRYHVTFYFSNGKEFDGKVTSKYNKEDYLEGLEDLFLKEKT
                     LLIDHLGMLIQTKYITHVKVIEVGSKIGADKKDT"
                     /name="hypothetical protein"
     gene            7034..7570
                     /locus_tag="L6487_RS29940"
                     /old_locus_tag="BCJMU51_p414"
     CDS             7034..7570
                     /locus_tag="L6487_RS29940"
                     /old_locus_tag="BCJMU51_p414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3967 domain-containing protein"
                     /protein_id="WP_236788722.1"
                     /translation="MTDKTDSSQSIYISKDVAMMLKIQESTLRKYCIMLEEHGYKFHKN
                     EHGHRGFLDNDVITLRKLIEIKSHPDMTLKQACNAIMTWVKEKDMSEVDTNVITENEQH
                     DERYSELKEMIQQQNELLKKMAKKLDDQQRYIDEKLEKRDQQLMGAIREMQEEKRVLLE
                     TASASQEKKWYKFWK"
                     /name="DUF3967 domain-containing protein"
ORIGIN
        1 aaagatttta atcgtcctaa ataccaggct ttaaaacaga tgctacgtgc cggagatata
       61 ctgtatatca aatctattga tcgttttggt agaaataaag atgatatttt aaaagagtgg
      121 aatgaaataa cgaaggagat cggtgcggat atcgttgtat tggacatgcc tttattagat
      181 acaagacaat ataaagatgt attaggctcg tttgtaagtg atttggtact acagatacta
      241 tcattctttg cagaagaaga aagacggaaa atcaaagaga gacaacgtga gggcattgat
      301 gccgcacaaa gacgaggtgc gaagttcgga agaccggctg ctacattccc gccaggattc
      361 gaagaagtgt acaagcgttg gaaagatgga gaaattaaag caatagaagc tatggaagaa
      421 ttagagttaa aacggactac tttctataaa ctagtaaatt cattcgaaaa taagtaaaaa
      481 agtaataata atattaaact tttacaaaat taagtcgaaa aagcaaagtt aaaaaagaca
      541 aacataccga taacaagcat tgatattaca cataatttta tgcctttcat gtcgaaaagt
      601 aaaagttaaa aaagtaataa aaaagcaaag tataaaaagt atacttttaa gtttgcttgt
      661 ttctatgaaa atccgttggt ataatagttt taacgtgttt gagtgtttca aacgaaaaga
      721 aaaaagccga gttggggaac tcgacttaag tctttctaaa agtatgggga tacttttaga
      781 ttagattttg tgtctcgaca acactttcga ctaattagca ctaccaatgc ttattagtca
      841 ttgatgattt tacgaatcat cttaacgatt atttttttat ggattgacac ctattttatc
      901 gacctttgac aatcaaataa ttaggtacca tttatatcta agattgaatt aacaacctta
      961 tttaacgtat gaaaaagtac tgtaggtcaa gttgtaaact ataaaaagtt tcttcttagg
     1021 aaaccttaag agaaaaaaat atagatacaa tttgtatcgc tttaatacga aaaaaagttc
     1081 tcgttcagcg aacttcctat gtgacaagtt tacaatgtat ttttcgtttt gacaatactt
     1141 taagtaaact catagttttt acatacattg aaaggtacct ctactatttg aggtgccttt
     1201 tttatttatt tttgaaagga tgatagaaaa tgatagggaa agatatgatc acattagaca
     1261 acccaaagaa agagtatttt cagcaatatg cacaaaataa taaagaacaa attaacgcca
     1321 atgcaagaag acgaagacaa caacaaaaag aaaaggtaga gaagtatgat agattggttg
     1381 cggcgttacg atctagagga attagtgtag aagaattatt gaaataagat ggacaagctg
     1441 tattaaggcc ggactaagac accaggagaa gatttgtata cggtctaggt tattcgtggg
     1501 gcttatgcta cagctgtcat tgtctagcgg tatcgcagtg cgtaagcacg agaataagga
     1561 gcagacatac accacaagcc ccgaacactt tcatgctctc caaaacaaca tgttttgggg
     1621 atacctagtg taacgtggcc gagtgcaacg gaaggtttta atgtttcgtt taaaaaatga
     1681 gtttcttcta ttacttgata tattagaaat aagtcggcta tttctctagt tttctagtaa
     1741 tgacaaattg tcgataacca tagtcatatc aagggtttaa gctattcgac ttattgctaa
     1801 taaggcttaa aaagccttcc aaaaattcca ctagaaatga ggtgagaaat tgaatcatga
     1861 ggaatttata aattcaactg aaatagatga taaattttta aagcaaatca ttgataatag
     1921 ggaatttaat gacgttatag tctcgtatta tgaacgacta cataaagaat cacgcgatga
     1981 tggaatgaag aagaagcagg aaaacgttaa aggttgcaat gatttttgga ttatagacaa
     2041 atatcatgaa catagattaa aagagtttaa acgaactaat ctttgcaagg acaagttttg
     2101 taacaactgt aaaaaggtta aacaagcttc acgcatgggt aaattcatac cacttattca
     2161 gccttacgcg gaaaatatgt atcaattaac gctaactatt cctaatgtta aaggcgatga
     2221 attgaaagat acactaagca agatgcaaaa agcttttgct cgtttaattg agtatttcaa
     2281 aggcaaaaag aaaataaacg gaattgattt tgaagtttta caatacgaag gcgctatacg
     2341 atctttagaa ataacattta acggtaatga gtatcatcca cattatcatg ctcttattgt
     2401 tttaaaggtt aataaattcc atgattgcat gataagaaaa cataaaaatg tttattcaat
     2461 agacttcact ggaaaacgtg aggcgcgttt atttagtgat atagaggttt tgatacagaa
     2521 ggtttggtac ttgattataa acggtaaacg gataacagaa acagcgatac agtcacttaa
     2581 aaaaggttat tcatgtcagt tggacaagtt tagcgaaaac gattttgtag agatatttaa
     2641 gtacatgaca aaagcaacaa cggaagacgg taaaacattg agttataagc aattcaaaac
     2701 gttgtattat gcgttattta aagttagaca aatacaaggg tatggcatct tttataattt
     2761 gaaggatgat gaagaaatta attttgatga agttaatgaa gcgtatgcgg aattcataga
     2821 ggaattacag acaaaagaaa aacctgttac ggtatcagaa gcaccagaac aggtcagaaa
     2881 agaaagtgac tatacagtgt ttagtcgaaa aacgttccaa aaatattgga agcaagtcat
     2941 gagcgaatag ccttactcat tccggcaagt ttgataaaag gctatcttta ccaccattaa
     3001 tcaaataacg gagggttttc taatatcatt atacataatt ttaattcgta tgatattagg
     3061 aaattccttc tatatcgtta attagtagag actatgaatt atttcgtagt ctttttttat
     3121 tggaaaggaa tgattgttgt tatatttaga ggtttataac aggaaaaagg cgcctgatct
     3181 cgtttgaatc agaacgaaat caagcgcctt tgggcgataa ttaaccaaga acaagttaat
     3241 taaataaatc taaaagtttt tggaagtact tatattatat cgtatatgga gttcccggac
     3301 agtaatttta gcctaaattt gcgtgaagca aatcattaat cataccacgt aaactatata
     3361 ttcgtaaata ggtaaatgtt aaaataagag taagaagaat gaaaagaagg tggaaaagtg
     3421 gaaagagaaa agtgttcttt gaaagaagct aaaaacgctt ataaccgaaa atggagagca
     3481 gcgaatcgcg ataaagtgaa gctgtataac gatagatact gggaaaagaa agctaaagaa
     3541 atggaggaac aagcatgtca gaagtaagta tggaaactgt tattaaagga aagcagcaga
     3601 gtgaactgtt gaagcattta gaaaaagtag gtattgaatt actggggcgt agagatgaat
     3661 tgttagagca gtgggaaaaa gaaggactta aagaagacag catttttgaa gatgatctta
     3721 aattcgttga agaattaatg aaccgaaatg acgaattaat gtttgatgta aaagtggaat
     3781 taattacaac tatggatgag attcatcatc agaaaatggg gtattgatag gatgttagga
     3841 aagtatgatg gattattaga aaaagaacgg ttacttaaac gaattgatga actgtcattc
     3901 gcgttagaag cttcaggtga ccctatgttg gaagcgatgg ttaaaaatag tggcaataca
     3961 gacatcgcgg ttgcaaaaga gttgattaca aaagggagtg aattaggtat gaatgagcac
     4021 gtatttaaaa aagcgttgct agcaaaagta aatgaacttc gtgaagaggt aacgaaacta
     4081 tgaaaaaaga caaacaaaaa gacctccctt ttaatcacaa agtagtattg agcgtactac
     4141 ctttaggaag atctaaagca ataacaacta aagaaattat ggcacgtagt ggaataaaag
     4201 acaagcgtgc agtatctgct attttaagtg aattagtgac tgtatataaa caacctattg
     4261 ctacaacgtc taaaaaagaa tttaagggcg tttttattgt acaggatgta atagattatc
     4321 agattggtaa agaaggcctt gtgagcagaa aaaggcatat agataagcgt gattcagctt
     4381 atacagaagc gtgcttggaa atattcaaag gtacagtgac attagatgag tcaaaaaaca
     4441 aagtggaagg aagtgttcgt gcatgcaaac aatcagcaag ttcttcaatg tagataacga
     4501 gagtaagtta aacgaattaa caatagcaaa aggggaattg aattccaaag aatcagaatt
     4561 acaaagtaag ctatctcgag tagaaagagc tctggaactg gcacaaactg acttaatgat
     4621 tgatgatagt acagctaata aaaaggttgt tgcaaaatat gaagaagctc aagagaagtt
     4681 ccaatcagag ttaatagcta ctcaagaaga acttaacaag gtttctgatg agattgcagc
     4741 tatcgttaag gaagaattgc atgctgaatt agtagatgcc gctaaacagg acgttatgct
     4801 aatcgaacta cctgaaaagt gcagaaaagc gaaaaaagta attaacaggg atctttatag
     4861 ggcgacgtcc tttgaagatg aaggtcgatt tagtagaaag tatttgcatg ttagtgctac
     4921 agtgatcgac gacgctcgta ctttcgccga tatacatcgt tttgctgaca aaccaaatct
     4981 aacacgaata gggtatcaac gcggtgaatt gatacgttgg ggtggcgata ataacctgtt
     5041 caaattgaat gatgaatatg aaacgatata tgaagaagaa gcacagaaaa tcaaagacaa
     5101 aacggataaa gagtatgagc agttcatgaa ggctttcaat aagtatttcg ctgtgtcgga
     5161 ttctcattaa tcaaaataca taagtcgcct aagggcggct tttttttatt aggagggatt
     5221 ttcatgagta aagatacatt aaaaaacatc tttcacgtgt attgttttta tacagtacgt
     5281 ttgcaaggcg atacagaacc acgtatgaga cgaaacaagc taaattatga taatcctata
     5341 caaaactatt atgaaagttt tatagactgt ctgagaggat tctgtgaggt taggagcgac
     5401 gtttcattgt attcgttcta tcaatttgtt gtagatgctg ttaattcgtt aaataagcag
     5461 gaaagaaagc tgatctatga gcggtattta tataaagatc actacaaatc agataggcaa
     5521 cattaccttg cgatggatat aacaccacag aattataaaa aacagatgga tccggtaaga
     5581 aggaagttaa ttaagaacct cggaatagaa gatttacagt tgaatattcc agattggatg
     5641 aaacgataac gggttaaatt acatgtatgg ttatattatt tttaaatttt attttgattc
     5701 tattgcattt tactatactc attgttatac tagataatgt aaacaacatg ttgatttata
     5761 ggaggttact agtttaatta tgaaatcact atatcgtatt cttgaattgg acaaagatga
     5821 agtttcttct tctgaaaaac taatcttgct aacgatagcg atctattcag acaacaagtt
     5881 tatcccattc tctattacag aattggagaa ttacacgaat ttgagtagag ctacggttac
     5941 aaggctcgta aaacagttag aagaaaaggg attcgtcgag gttcaaagga atggaacagc
     6001 aacaaacaat tacaaagtaa cactgtaaaa acaaagaaag agcaggagat tgcggctcct
     6061 actctttcaa aacacattat cccttatgga aaggatgtat tagtatgtta ccacaaaaag
     6121 aacagttagg acatcttgct gagaaaactg aattagttaa ggatttagct tggattgcac
     6181 atgatttact taccgaggaa gattacacca aagagaatgc agcggaagcg ttaattaagg
     6241 tcattaatag ggaattatca tacgtttcca aagtgcgtta aacgggatta tatgtaaata
     6301 aaacaattcg gaggtaatat tcatggcaaa tacagttgct aaagataaat ttgagatcgt
     6361 agaaaagttt gtgaatgctt gtatgttaaa aggtgtttcg gaagataaat cattcaaatt
     6421 agtagaggaa ttatgtacac ttattcaaca aaaacgtcat gatgaatggt ttggattagt
     6481 agatgaagca cttaaagaag cgggacatga agaaggtgta acgttatgag tgaagtagaa
     6541 atggttttac acgaattcga agaactatta cataaaatca tatcagcagc gcaacatggc
     6601 gatgaacaag cgatgaatca tttagatata gtaaaagcat ggttaaagga caattaaggc
     6661 gtggtgatcc acgtcttttt tttatactgt cttggggaat cctactgata tattttgaaa
     6721 gtaggtggac taaatggctg aagaacatag ataccatgta actttttatt tttctaacgg
     6781 aaaagaattt gatggtaagg taacaagcaa atacaacaaa gaagattatt tagaagggtt
     6841 agaagatcta ttccttaaag aaaagacatt actgattgat catctaggaa tgttaataca
     6901 aaccaaatat attacacatg tcaaagttat cgaagttgga tctaaaatag gagcagacaa
     6961 aaaagatacg tgaattatta aggaaaagga tatatcattg ttataacgaa tgttttggta
     7021 gaggtgaagc gttatgacag ataaaacaga cagctctcaa tccatatata taagcaaaga
     7081 cgtagcgatg atgcttaaaa ttcaagaatc gactttacgt aaatattgta tcatgcttga
     7141 ggaacacggc tacaaatttc ataagaatga gcacggccat agaggattcc ttgataacga
     7201 tgttataacg ttaagaaaat taatcgagat taaatcacat cctgatatga cattaaaaca
     7261 ggcctgtaac gcaattatga cgtgggttaa agagaaagat atgtcagagg ttgatacgaa
     7321 tgttataacg gaaaatgagc aacatgacga gcgatatagt gagcttaaag aaatgattca
     7381 gcagcagaat gaattattaa agaaaatggc gaagaaatta gatgatcagc agaggtatat
     7441 tgatgaaaag ttagaaaaac gagatcagca gttaatggga gctatccgag aaatgcaaga
     7501 agaaaaacga gttttgttag aaacggcttc cgctagtcaa gaaaagaaat ggtacaagtt
     7561 ttggaagtag gattccctaa ctatggggag tcctttttta ttttaaagtg agtttggatc
     7621 acttagaggt agattaaaca gaaagccctg caggtaacat cctcagaaac gcacaagaaa
     7681 agtggtgcgt ataactgggc actcgctttt ttctcgtgaa taagatctca ttgaatggag
     7741 taattttgta tactactccg ttggaatata gaacgtttta cggagcgttc caatatcgtt
     7801 acctttgcaa aacaaacacg aataccacgt tactttaatc cgcacacaag gcaccctttt
     7861 tatgcagtac tcattgtagt attgcacccc ttttaatgtt gtactgcaaa acaaacacaa
     7921 ctcaactaat aaagaacaaa gcacatatac cggtaattga atggtaacgg gtgttaagga
     7981 atgataagga gtattagtgc aaaatgaatg tagtagtatt ttagtaattt gcaaaacaaa
     8041 cgaactcaat taatgtattc gtaaaagtac acatttacga ataagaaaaa gaataagaaa
     8101 aagatattgt tttgtgttcg tataagttgt atgattagtt tacaaacatt cgtaaattga
     8161 aaaacacttt tacgaacaaa ggggaagagg aaaatgaaca aacgtttcgc ttatatccgt
     8221 gtatcagata aaactcaaaa cgaaaataga cagttggacg agatgttgca gcaggagatt
     8281 gaagaaagag atatcttcat tgacaaggca agcggt
//
