LOCUS       NZ_AP022954             8220 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain J39 plasmid pJ39_2, complete sequence.
ACCESSION   NZ_AP022954
VERSION     NZ_AP022954.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203334
            Sequence Read Archive: DRR206374, DRR206398
            Assembly: GCF_021655175.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8220)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 506x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655175.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 06:50:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,874
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,455
            CDSs (with protein)               :: 5,455
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 267
            CDSs (without protein)            :: 267
            Pseudo Genes (ambiguous residues) :: 0 of 267
            Pseudo Genes (frameshifted)       :: 140 of 267
            Pseudo Genes (incomplete)         :: 134 of 267
            Pseudo Genes (internal stop)      :: 53 of 267
            Pseudo Genes (multiple problems)  :: 54 of 267
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022954.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8220
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="J39"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pJ39_2"
                     /geo_loc_name="Japan:Tochigi"
                     /collection_date="2006"
     gene            join(8062..8220,1..279)
                     /locus_tag="L6483_RS29145"
     CDS             join(8062..8220,1..279)
                     /locus_tag="L6483_RS29145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000094076.1"
                     /translation="MSSFRWYEENEVIGKTIIEEDDLTLLEAGKIYIDAIFCERIRFPV
                     QKNEQPIGFNFVYEYNVKKYFGLYDRSFMKIIKQEDLEEYHMRMIDFHVHRLSLGEELD
                     DCPIIGICYGSGPLITPRLEQVYDVSKTKAYRKYRLEKLFD"
                     /name="hypothetical protein"
     gene            530..1150
                     /locus_tag="L6483_RS29150"
                     /old_locus_tag="BCJMU39_p21"
     CDS             530..1150
                     /locus_tag="L6483_RS29150"
                     /old_locus_tag="BCJMU39_p21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000963189.1"
                     /translation="MAIDFSQADRNARKRDLEKEKFFSDEEWELANDLQSRANARGMKL
                     VPERKIKNRAKFAQMLQDNVDYLEEIDYLDDKEIIFLWKISRKVGFLSNCLVDDIHKKS
                     PVPLTQTDIATLLKRTKNNVNPIVKSLVAKGILAVSKTGVQGNNSRANVYFMNPNILYA
                     GSKDDVNLTLQAMFSKVPKALKDMPVKLFELSCHSEEEEEKTN"
                     /name="hypothetical protein"
     gene            complement(1835..3265)
                     /locus_tag="L6483_RS29155"
                     /pseudo=""
     CDS             complement(1835..3265)
                     /locus_tag="L6483_RS29155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053955.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /name="IS4 family transposase"
     gene            complement(3595..3972)
                     /gene="qacH"
                     /locus_tag="L6483_RS29160"
                     /old_locus_tag="BCJMU39_p26"
     ARG             complement(3599..3972)
                     /name="qacH"
                     /gene="qacH"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacH"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(3972..4523)
                     /locus_tag="L6483_RS29165"
                     /old_locus_tag="BCJMU39_p27"
     CDS             complement(3972..4523)
                     /locus_tag="L6483_RS29165"
                     /old_locus_tag="BCJMU39_p27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_000028030.1"
                     /translation="MSISKHANKKKNILIAASEIVKEEGVVKLTLEAVAQRAGVSKGGL
                     LYHFPSKEALIKGMVEDWTNNYFECINTLVNNDDDNAIGKWNRAYLKSTFSDLENNNLN
                     SALMATMFINPDLLDEFRQRYDILHTKLITDGIDPVKITITRLSIDGLWFSEIFGMAPL
                     NEELKTQVFDELINMIQEDE"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            5718..6830
                     /locus_tag="L6483_RS29170"
                     /old_locus_tag="BCJMU39_p28"
     CDS             5718..6830
                     /locus_tag="L6483_RS29170"
                     /old_locus_tag="BCJMU39_p28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002019875.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_000036645.1"
                     /translation="MSKITNDIDLKIDEVYEKVKDMKITFDENNTTKKQLTDSTINTYC
                     GIVKSYNNWLIQTHGISVDRAKPRHAMEYLETKIQAFEKGNASAFSMRQMAHAFHSFRV
                     ASAETGTFKHTCKVADKRDMLNTLTERGIFRKSADSTVLKANHSDYEKVQKELNVATTS
                     KHLREDIKTVHELQRYAGMRVHEAVKLKVSDIDFKSGTVYIKGKGGHERTVKIESKELL
                     EKLKNLCTGKSERSQVIIARKRDKNQLTNEEFGKRAKEEIRGAAVNAGVDRNGKRYTTH
                     SGRKAYAQAQMDKYKSYSKNQLKKEVAKRCQDKKTKQKYDAVIKNIRKKITEGSKNEGR
                     KLTNKEMCQFLVSLDLGHYRLDVVRYYADY"
                     /name="tyrosine-type recombinase/integrase"
     gene            7641..8048
                     /locus_tag="L6483_RS29175"
                     /old_locus_tag="BCJMU39_p29"
     CDS             7641..8048
                     /locus_tag="L6483_RS29175"
                     /old_locus_tag="BCJMU39_p29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000037919.1"
                     /translation="MSKKISIEEMTQEMKEKFTISLEDAHKILLDHGVTKSTNTQVTMR
                     WIRDGKIDALLIGKGRVETRKWLINKESLMEFVAFERLTLGDFKAMKEELEMLRAFKFE
                     VEKKPKRATGTRQKKDAATNTSKEKKEVPTK"
                     /name="hypothetical protein"
     ARG             complement(3596..3972)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="60.75"
                     /coverage="116.82"
ORIGIN
        1 tttaattttg tttatgaata taatgtaaaa aaatatttcg gattatatga cagaagtttc
       61 atgaagataa ttaaacaaga agatttagaa gaatatcaca tgagaatgat agattttcat
      121 gtgcacagat tatctttagg ggaagaatta gatgattgtc caatcattgg tatttgttat
      181 ggaagtggac ctttgataac accgaggttg gaacaagttt atgatgtttc gaaaacaaaa
      241 gcatacagaa agtatcgatt agaaaaatta ttcgattagg taaagcatct tacaaaattt
      301 gagtttttaa gctagaattg aatagacaag cataaaaaaa agccgagtgt tggagcactc
      361 gactttgggt gattcatcgt aatttatcac caaaacgttg atttctagtt ttaattttaa
      421 tgaattttca tttaaaaatc aagggtaaaa ccctttacgg ataataaaaa aataacggtg
      481 aatctcctag aatgcttgtt gcattttctt caggagagga gcaaaatcaa tggcaattga
      541 ttttagtcaa gcggatcgaa acgctagaaa aagagactta gaaaaagaaa agtttttttc
      601 agatgaagaa tgggaattag caaatgattt gcaatcaaga gctaacgcgc gaggaatgaa
      661 acttgttcca gagcgtaaaa tcaagaatcg agcaaaattt gctcaaatgc ttcaggataa
      721 cgttgattat ctcgaagaga ttgattattt agatgataaa gagattatat ttttatggaa
      781 aatcagtcgt aaagttggtt ttttatcaaa ttgtcttgtg gatgatattc ataaaaaaag
      841 tccagttcct ttaacacaaa cagatatcgc aactttgttg aaaagaacta aaaataatgt
      901 caatcctatt gttaaatctt tagtagcaaa aggcatactg gctgtttcaa aaacaggtgt
      961 tcaaggtaat aattcgagag caaatgtgta ttttatgaat ccgaacattt tgtacgccgg
     1021 tagcaaagat gatgtcaatt taacattaca agcgatgttt tcaaaagttc ctaaagcatt
     1081 aaaggatatg cctgtaaaat tattcgaact atcatgtcat tcagaagaag aagaagagaa
     1141 aacaaattaa taataattaa tacatgtatt ttttaataca tgtatttttt tatctttcaa
     1201 ttatgtaaaa taatgtaaaa aatgtaaaaa atccggtgtt cttaaattca agaactatgg
     1261 ttcttaaatt caagaactac gaaaagtgca acaaaccatt gatattattg actttttaat
     1321 acattaacaa tgttgttcta tctcactctc tattaacgct aattttgagt aaagaaacga
     1381 gtagtatttt ttgcaaaaac cgcaaaaaat ctccactctt tatcttgact caaaattctc
     1441 cgcaattggc atggcttgtg ccaagcgata aaccaaaaaa gttatcactt tttcggatta
     1501 tcacttgaac aatccatttt aattgctccg tcgcttagtg cgtacatttc cgttctatca
     1561 atattttgtg taataaaaac ccttggggct agccccaaac cccagccagc tcaacccaaa
     1621 aaagcatatg aaaaccgtat caaatttaat acggtaaaag aataaaagac ttaaaaacat
     1681 ttttgcttag gaaaggggta gcgtcacatg cccatcaact taagaacgaa aacaaaatga
     1741 actttcgttc aaacaaacta aaaaacacgt aatgtacttt tggtattgaa aagtacacac
     1801 accaaataaa ctctaacgag tttcatttta aaaattatgc agctttcttt tgttttccaa
     1861 acccattata ctcatagaca acacccataa tatcaaagat agttttcttc tcatatcgat
     1921 gagatttccg tccattcttc tgtaggaggt cgaacaaacg gattaaaatc tttgttagat
     1981 tttgggtgtt tctctgtatc gcttgatata acagcgatac atgatcttga atcatcccaa
     2041 tggctttata ttcacttaat tctcttttgt tcttttgcaa aagaagttta cgcatcttaa
     2101 acatcgtaga agaacagata aagatggcaa taagtcttcc atacacatga cattctaatc
     2161 gctctttttt gatagtttgc caatgatgaa tttgaaataa ggatttccac gttttaaaaa
     2221 taatttctat ttgccagcga agggagtaaa agtcatgtat ttgttccatc ggaacaactc
     2281 cttcaggcgt atttgagaca tatatgttga tacccgttaa tcgtttactc ttttctgaaa
     2341 atgtaatacc ctttttactt tcagtgtaac tttgtttttt ctacgttcct gtatctgttt
     2401 gtctgttaaa cgatagataa ttacacgtgt gaatagtttt tgattctttc caatgtaagc
     2461 ttcttttatt tcgtatgttt gaccgggttt taaattgttc ataatatgtt ctaaatcaac
     2521 ttggatatac tgtgattgct ttttacggtt ccgtttcgaa agtattctgg aaacggattt
     2581 ttacatacac ggtatggttt aattttaatc tggatatata ataagcacct cgttgatcca
     2641 tttgatctaa gtcttctaat gaaaaataac ctaaatcacg gatacataaa tcgcttggac
     2701 gtaatgtgtc taagcattct gttccaaagg tcttatcatt atttttcctg gacctatctg
     2761 aaagtttaga aactgtccgc tatgtaagtc atattctaat tgaatcttga tacccgcatt
     2821 ctgtgcacaa ccacctgatc caggatatac atgggctaaa tgttttggta cttggaaaat
     2881 cgtcgaatct aaaatacgaa ttcgttgaac atatgtgagc gccgcgcttg aaatggccga
     2941 tgtttcacag attttacttt tccatactgc cgaaaaaata tatttcaaaa ttcaaccgct
     3001 ttttatcaaa gcgtttattg agtccttctg gactcataag agtgcctgtg gccgcatgta
     3061 attgactgca tagtcgaaca agcgagtcac tcgctgtacg ttgacttacc caaatacata
     3121 tggtagctaa ttcatttcca gaaaactttc gttttctttt tacaaaacct aattcttttg
     3181 cgagttcttc caaaagagaa ggggttaaat gacgatacag ttcttcagaa aatagttgta
     3241 attcatcttg aatcgaaaga ttcataaaaa atgtcaccct ttctagctag ctttttagaa
     3301 agaataacgt tttttgtcac ttgggggtag tgaaaattct taagttgatg ggcatggggt
     3361 agcgtcaggg aaatgcggat ttacaacggt aagcatttga tacaagtctt atgagacagt
     3421 cagaagtcat ttaaaacgct aatttcatta tgtctcaata aactctactc tattggaatt
     3481 gtgaagtgat acacttaaac taacgtacgc aggtttgttg aataaggact tcaaaaaata
     3541 cccttaatta agctaaaaag tccttctctt tattaagaag gactttttta tgtgttattc
     3601 cattgtctca ttcattgtaa tagtttcgct tgcttcatga ccagattctc catgtcctgg
     3661 tccaaacaaa ttcaatatta caactcctac aagaatcagt gttattcctg caatgctagc
     3721 catgttaagt ttttccttcc aaactaaaac tgaaattact gtagtcaaaa caagtcctaa
     3781 acctgcccat aatgcatatg cagtgtttaa ggggattgtt tttagcgaca aggataaaaa
     3841 gaaaaaacaa ccaacgaaag ctattattac ccctattgta ggatagagtt ttgaaaagcc
     3901 ttcagatgct tttaataggg aagagccaat aatctccccc actattgcaa gtgctaaata
     3961 tagatatgac attattcgtc ctcctgaatc atgtttatta gttcatcaaa aacttgtgtt
     4021 tttagttctt catttaatgg tgccattcca aatatttctg aaaaccaaag accatctatg
     4081 gataatcttg taattgttat tttgacagga tcaattccat cagttattaa ctttgtatgc
     4141 aaaatatcgt atctctgccg gaattcatct aataaatctg gattaataaa catagtagcc
     4201 attagtgcgg aatttagatt attgttttct aaatcggaaa atgtagattt aagataagcc
     4261 ctattccatt ttcctattgc gttatcatcg tcgttattaa ctaacgtatt aatgcattcg
     4321 aaataattat ttgtccaatc ttccaccatt cctttaatta atgcttcctt gctaggaaaa
     4381 tggtataata atccaccttt actcacgcca gctctttggg cgactgcctc taatgtgagt
     4441 ttgacaactc cttcttcttt tacaatttca gatgcagcaa tcaatatatt ttttttctta
     4501 ttagcatgct ttgaaataga cataataacc tccataactt agatacatat aatataccgt
     4561 ctggacggtt tttttgtcaa tcgaattaaa gctcttattt aatatcactc tttcacataa
     4621 aaataatcca ataaattaat ctaaagattg tgaagaaatg tacttaaagt aaagggagct
     4681 ttagttcaac agtcgatttc ttaaaatcgg ctttatttat gggtgaaaat tacattaccg
     4741 ttgtaaatcc gcattttcag gactctaccc cggaaagaaa aacatttaag gtcgtaagac
     4801 cttttcaaaa gcataaaaaa ataaggtttt ttcatcaaaa tcagttgatt aatggtaaaa
     4861 tttgtatata ttcatagtaa ttttaaaacc aattacttat ttgtttaaac gcttgcggtt
     4921 aagcaaatat gaaatacgaa attttatact atgaatcttg ggacgtgatt caagtaatcg
     4981 tgtattttac aaaactaaaa aattcacgtc ataaaaatga aaggggatca ttacaatagc
     5041 aaagcaaaat gtttttaaga cgtgggaaaa aataatctaa gaaaggagca atataaagct
     5101 ttcccaagta gttttataaa tagctttaaa agaaaataga attcttgctt gcaagaatgg
     5161 aaaaaaatat tataaaaaat aaaaatttaa ggagatgaca aatcaaaaaa tacgaaggga
     5221 acaagtttta caattaaata tttttacaac tgaataaatg taaatctgta acaaaggttt
     5281 ttaaccacaa aacaaatcgc aagaacagta atttctgggt tactcaaaaa ttccagaccg
     5341 caataccgtt gtacgctcat ttgactatac attttggtgc aaaatgtatg ggagagcctt
     5401 ggagtctatt tatagacgcc aatcgcaaca agatggcgta gctatcttga gcctttgtct
     5461 tttaatcttt tgaccttgat ttttgctttt ggtcttttga ttttgacttt gactctagtg
     5521 agcttaaatc gcccgaaggg agatttcgcg attgcttttg tgtgtttaat ttgggtggtt
     5581 cggtctattt tttttggtct ttaaaaaaaa tagatcgaac ccacccaaat gagacctcgt
     5641 ttttgttgtg ccttgatatt gtaaggttca cgggggttgg gttcttgctc ttgattttgt
     5701 atataggggg tgacgaaatg agcaaaatta caaatgatat tgatttaaaa attgatgaag
     5761 tctatgaaaa agttaaagat atgaaaataa cttttgatga aaataataca accaaaaaac
     5821 aattaactga ttcaacaata aacacatatt gcggaatcgt gaaatcatat aacaattggt
     5881 taatacaaac gcatggaatt agcgtggata gggcgaaacc tcgccacgca atggaatatc
     5941 tcgaaacaaa aattcaagcc tttgaaaaag gaaatgcttc cgcattttct atgcgccaaa
     6001 tggcgcatgc gtttcattct tttcgtgttg cgagtgctga aacaggaacg tttaaacata
     6061 catgtaaggt cgctgataaa cgagatatgt taaatacact cacagagcgt ggaatcttcc
     6121 gtaaatcggc tgattcgacg gtattaaagg caaatcattc agactatgaa aaagttcaaa
     6181 aagaactaaa tgtagcaacg acaagtaaac atttgagaga agatataaaa acagtacacg
     6241 aattacagcg gtacgctgga atgcgtgttc acgaagccgt aaaactaaaa gtgagtgata
     6301 tagactttaa gagtggcact gtctatataa aaggcaaagg cgggcacgag agaacggtta
     6361 aaattgaaag taaagaatta ctagagaagt tgaaaaattt atgcactgga aaaagtgaaa
     6421 gaagccaggt aattattgct cgaaaacgtg ataaaaatca attaacaaat gaggaatttg
     6481 gaaaacgtgc aaaagaagaa atacgaggtg cagccgtcaa tgctggggtg gatcgtaacg
     6541 gaaaaagata cacaacacat tcaggtcgca aagcatatgc acaagcgcaa atggataaat
     6601 acaaaagtta cagtaagaat caattaaaaa aagaagttgc aaaacgttgt caggacaaga
     6661 aaacaaagca aaaatatgat gcagttataa aaaatatccg taaaaaaata accgaaggtt
     6721 cgaagaacga agggcgtaaa cttacaaata aagagatgtg ccagtttcta gttagcttgg
     6781 atttgggtca ctatcgttta gatgttgtac gatattatgc tgattattga taggttttga
     6841 cggaaaatcg ggaagacgag tgcggagcac agttggtact ggcgcactgg cttagaaaca
     6901 caagggctag agcctcgttg tcaagccatc tttcccggct ttacaatgag aaagccttgt
     6961 gttagcctct catggcaatg ccagtacctt gtgcggattc gaggaaatcg ccaaaaaacg
     7021 ggtcgatgaa ctaagaagat acacgagagt tttccaaatc ctcgccattt ctcgcttttt
     7081 ttcgaaaatc aaaaaaaaat gaaacgccta ttaaatttac tatacagtaa aagtatttta
     7141 aaatacattt attgtatagt aaattttttt gagtaaaagt attttaaagt aattttaata
     7201 gttttacaca atagtaaatt tatataccta aattagattt acacaaaagt aaaaatatag
     7261 ataaaaatac atgtattgtc tccgcaattt atttttagta atcgcaaaaa atcgttgatt
     7321 taacaacgtt taagagatat aaaatacatg tacaaaatag taaagtcatg gcaataaata
     7381 gatttacaca aaagtaaaag taatgaccta aaatggattt acataaaaat aaatttaatg
     7441 tataataaat ttaaagaagg atatatataa ttttgagtaa atataattaa tagtaaatgt
     7501 aaaatagatt aaatatattt tttaataaat ttatttaaaa ttatatttac ttttcaatac
     7561 atttactgta tattaaaaat atagatggat acatttaatt aaaaatacat gtacttaatt
     7621 gtaattagga ggaaaaaaca atgtcaaaaa aaatctcaat tgaagaaatg acgcaagaaa
     7681 tgaaggaaaa attcacgatt tcactggaag atgcacataa aatccttttg gatcatggcg
     7741 taacgaaaag tacgaataca caagttacaa tgcgttggat tcgtgatggg aaaattgatg
     7801 cattattgat tggaaaaggt cgtgtggaga ctcgaaaatg gcttattaac aaggaaagtc
     7861 taatggaatt cgttgcattc gagcgtttaa cgttaggtga tttcaaagca atgaaagaag
     7921 aattagaaat gttacgtgct tttaaattcg aagtagagaa aaagccaaag cgtgctactg
     7981 gtacacgtca aaagaaagat gcagcaacaa atacttcaaa agaaaagaaa gaagtgccaa
     8041 caaaataaag aggtgaattg catgtcatct tttagatggt atgaagaaaa tgaagtgatt
     8101 ggaaaaacta taatagaaga agatgatttg actttgctag aagctgggaa aatatatatt
     8161 gatgcaatat tttgtgagag aataaggttt ccagttcaaa aaaacgaaca gccaattgga
//
