LOCUS       NZ_AP022951             3089 bp    DNA     circular CON 11-MAY-2024
DEFINITION  Bacillus cereus strain J10 plasmid pJ10_5, complete sequence.
ACCESSION   NZ_AP022951
VERSION     NZ_AP022951.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203333
            Sequence Read Archive: DRR206373, DRR206397
            Assembly: GCF_021655155.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 360x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655155.1-RS_2024_05_11
            Annotation Date                   :: 05/11/2024 05:24:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,843
            CDSs (total)                      :: 5,691
            Genes (coding)                    :: 5,445
            CDSs (with protein)               :: 5,445
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 159 of 246
            Pseudo Genes (incomplete)         :: 101 of 246
            Pseudo Genes (internal stop)      :: 38 of 246
            Pseudo Genes (multiple problems)  :: 44 of 246
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022951.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="J10"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pJ10_5"
                     /geo_loc_name="Japan:Tochigi"
                     /collection_date="2006"
     gene            complement(join(2051..3089,1..206))
                     /gene="mobV"
                     /locus_tag="L6486_RS29060"
     CDS             complement(join(2051..3089,1..206))
                     /gene="mobV"
                     /locus_tag="L6486_RS29060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000951567.1"
                     /translation="MAHVEKYTKGSVQGLSIHWDRKTENHSNQEIDNERSNLNYDLCEK
                     EGDTLSRMNDRLREVHCLNRKDVKVCSDWVVTLPENLKGTSEKEQREFFEKTYEFLANR
                     YGGEKNVLSANVHKDETTPHMHFAFMPVVWDEKKQREKVSAKEVLTRKDLKTFHQDLDT
                     FLKKEIPHIYKDGILNDKTIGVDTVKDLKKHSLEIEKQKKAMDAEVKIKEKDLERKIQS
                     LDKEFESKKKKLLDLSEQLPQEIKINAKGKEKKTEVVKKGLFKTETITKNTGNWIIGTN
                     ELKRVQKMVNAAYMVKRDYERLQSTDLVEENKKLHLQVEGLSNNLKASHQINAELRERN
                     KELHTKIGSLQAHINDLKINVKVLYQQTKKVFKEQFKTFRGLVKNELVGREVEDYFERE
                     HKNEMTKQRGYDMER"
                     /name="mobV"
     gene            1563..2021
                     /locus_tag="L6486_RS29065"
                     /old_locus_tag="BCJMU10_p51"
     CDS             1563..2021
                     /locus_tag="L6486_RS29065"
                     /old_locus_tag="BCJMU10_p51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125938.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_001125937.1"
                     /translation="MPKQKTENEIIYNGKSRWRNLDTGEIIETDEVIKKTPRNGFMITY
                     LTAIVQLIDSLGNKKMQVVKYILENMDKSTNTLIITTRELTEKSKVSRQTVIDTLKTLE
                     QAQIITRRTGAIMIHPSLVHRGKDTKEKYLLARFEDFNNNKAPINAVE"
                     /name="replication/maintenance protein RepL"
     Typing          1564..2021
                     /name="rep_cluster_1950"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..347)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.13"
                     /coverage="99.0"
                     /evalue="2.28e-71"
     Typing          complement(2055..3088)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.71"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tttacatctt tccgattcaa acaatgtact tcacgtaatc gatcattcat gcgggaaagt
       61 gtatctcctt ctttttcgca taaatcataa tttaaatttg accgctcatt atcaatctct
      121 tgattcgaat gattttctgt ttttctgtcc caatgaatcg ataatccttg tacgctgcct
      181 tttgtatatt tttctacatg agccacaagt aagcccccct tttggtaatc agcatagcac
      241 gaagattgag agaatccgat aatttggtct agcacgttat accaaatttc atttggacgt
      301 gctctgcgag gctcgttggc ttcgccaaac catgccttgc atggcaaaaa aacctcggac
      361 tttgtccgaa cctacaaggg gaactcttcc ccttgacccc atcaatcgaa ctccccaacc
      421 cctcaatcct tgagggactc cctcgccggt tggcaggcca aaacgtttga cgttttgaca
      481 ggccaagagg gtgaagaatc aattgcacgt gtcaattcct taatgctcct ttcatggatc
      541 agcacaaacc gctgacccct tccagtccgc ttaccattga cacttaccgc taatttttcc
      601 ttttccaaca atcgctttgc aaattactcc aaacatatgt gtttcctatt cgaatgaata
      661 taagagtaaa gtacataata aatatgaaat ataccttcaa ccttcaactg ttacccctaa
      721 aaagaaccct atccaacatt agggttcttt tttcatacca agccaaaaat aaaaaaggct
      781 tatccagtta tatacacatt tttgtcccct gcatacacta cttatgtaca actgcctact
      841 tgataaattg ccatacaaca gagtgattga aaggggattt tatgaattat tcatttaata
      901 aatctgataa attaatccct tttaaaaaga actacgggtg ttcaaaaaca gcctgtaggg
      961 acccttgtag ttcaatacca ataaaaaagt caaaatccac tatataggat tttgactttt
     1021 ttattggtat cccccttgtt tttgggtgtg agtgctggct gggggtttgg gggctagccc
     1081 ccaggaactt aacgtaattt tatatggcat tagtgaaatt cttgaacgat agatcaaggc
     1141 ggttcggcag gtacaattgc tagtccaagt cgtgcaactt aaatcctcgt ttttggtatg
     1201 ttcggcttgg cgacatgctc acacctgccg aatttcacta tgcaatagca aaagagtgga
     1261 gattttttgt ggttttctca aaaaatacta ctcgactatt gcattgtgga aagccctcta
     1321 aaaaaaataa aaaaagaata tatttaaggc gatatttatc gccttaaata tatagaaaag
     1381 agtaagcagg gggtaaaaag tagattttaa gctgatatat agctattgaa gggcattttc
     1441 tttggtctaa ttttttatac tttttggtct aattttttat accttttggt ctaatttttt
     1501 ttgacctttt ggtctaatta tgataaaatg gaaatacaaa aatataggag aaaggtgata
     1561 atatgccaaa acaaaaaact gaaaacgaaa taatatataa cggtaaaagt cgatggagaa
     1621 acctagatac aggtgaaata atagaaactg atgaagtcat aaagaaaact cctagaaatg
     1681 gttttatgat cacatattta actgcaatcg ttcaattaat agatagttta ggtaataaaa
     1741 aaatgcaagt tgtaaaatac atattagaaa acatggacaa atcaacaaat acgttaatta
     1801 taactacaag agaattgacc gaaaaaagca aggtatctag acaaactgta atagacacat
     1861 taaaaacact ggagcaagcc caaataatta ctagaagaac tggagcaata atgatacatc
     1921 caagccttgt tcatcgtggt aaagatacaa aagagaaata cttacttgct agattcgaag
     1981 attttaataa taataaggct ccaattaatg cagttgaata aaccgcccaa gaattgattc
     2041 taaggcggtt ttacctttcc atatcatatc ctcgttgttt agtcatttcg tttttatgtt
     2101 cacgctcaaa gtaatcctct acttcccttc caactagttc attcttaaca agccctctaa
     2161 acgttttaaa ttgctcttta aagacttttt tcgtttgttg atataaaacc tttacgttta
     2221 tcttcaaatc gtttatatga gcttgtaacg agcctatttt tgtatgtaat tctttatttc
     2281 tctctctcaa ctccgcatta atctgatgag atgcttttaa attgttagac aagccctcta
     2341 cttgcaggtg taatttttta ttttcttcca ccaaatctgt actttgcaaa cgttcataat
     2401 cccttttgac catataggca gcgttcacca ttttttgtac tcttttcaat tcgttcgttc
     2461 caataatcca attcccagtg tttttggtta tcgtttctgt tttaaataat ccttttttca
     2521 caacctctgt ttttttctct ttcccttttg cattgatttt gatttcttgt ggtaattgtt
     2581 cactgagatc taaaagcttt ttctttttac tttcaaattc tttatctaaa gattggattt
     2641 ttctctccaa gtctttttct tttatcttta cttccgcatc catcgccttt ttttgtttct
     2701 ctatttctag agaatgcttt tttaaatcct taacggtatc aacaccaatc gttttatcat
     2761 ttaaaattcc atctttgtag atgtgtggta tttccttttt caaaaatgta tctaaatctt
     2821 ggtggaatgt ctttaaatct tttcttgtta aaacctcttt ggctgaaact ttttcccgtt
     2881 gttttttctc gtcccaaaca acaggcataa aagcaaaatg catatgaggt gttgtttcgt
     2941 ccttatgtac gttagcggac aatacatttt tttcaccgcc ataacgattc gctaaaaact
     3001 cataggtttt ttcaaaaaat tcacgttgtt ctttttctga agtccccttc aagttttctg
     3061 gtaacgtaac aacccaatca ctacaaact
//
