LOCUS       NZ_AP022950             3453 bp    DNA     circular CON 11-MAY-2024
DEFINITION  Bacillus cereus strain J10 plasmid pJ10_4, complete sequence.
ACCESSION   NZ_AP022950
VERSION     NZ_AP022950.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203333
            Sequence Read Archive: DRR206373, DRR206397
            Assembly: GCF_021655155.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 360x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655155.1-RS_2024_05_11
            Annotation Date                   :: 05/11/2024 05:24:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,843
            CDSs (total)                      :: 5,691
            Genes (coding)                    :: 5,445
            CDSs (with protein)               :: 5,445
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 159 of 246
            Pseudo Genes (incomplete)         :: 101 of 246
            Pseudo Genes (internal stop)      :: 38 of 246
            Pseudo Genes (multiple problems)  :: 44 of 246
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022950.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="J10"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pJ10_4"
                     /geo_loc_name="Japan:Tochigi"
                     /collection_date="2006"
     gene            join(3203..3453,1..7)
                     /locus_tag="L6486_RS29040"
     CDS             join(3203..3453,1..7)
                     /locus_tag="L6486_RS29040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_236790439.1"
                     /translation="MSNAEGLRVLVDIDDLPTHQQIRVKRVAEGLTQTHLVELLGLPNV
                     PFLSYIETGAKPIPKWLETGMKRYLYEEVYKDGQLQKNVD"
                     /name="helix-turn-helix domain-containing protein"
     gene            join(3432..3453,1..188)
                     /locus_tag="L6486_RS29045"
     CDS             join(3432..3453,1..188)
                     /locus_tag="L6486_RS29045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236790440.1"
                     /translation="MVSYKRMLIEVDTIPDTEDGRKQADFLSKLKEAVTREEQSHVPYK
                     SLDVVAERLEDDSYRCTPNKLLQS"
                     /name="hypothetical protein"
     gene            1029..1919
                     /locus_tag="L6486_RS29050"
                     /old_locus_tag="BCJMU10_p41"
     CDS             1029..1919
                     /locus_tag="L6486_RS29050"
                     /old_locus_tag="BCJMU10_p41"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061684760.1"
                     /translation="MTTNRQLNMGEISSIQLDRLTIVGTIRRDKENYSPLFMKLLNGID
                     KRLQVDPYYKNHLYDRKIGVRTEESTAFIELSKNKTMYASDFRLDFNPTKCNEEDIKYI
                     KEILHYIDNKKATRIDIAADFLHQNFSQYHIEDGRRRKTNRITGASGRLETEYRGVRES
                     DDYIKLYDKSRERFDKKWEKVDYEWFRLEETAKGKKAKDWTNYEWFENIRLVPDHKNPI
                     FPEDVKATTKSQAYSVIMGFQNITEFSKGTQTKIREVIREAKFHGEVNIYEQIKKAPHQ
                     QMLNDALEKIRFFLK"
                     /name="hypothetical protein"
     gene            complement(2060..2875)
                     /locus_tag="L6486_RS29055"
                     /old_locus_tag="BCJMU10_p42"
     CDS             complement(2060..2875)
                     /locus_tag="L6486_RS29055"
                     /old_locus_tag="BCJMU10_p42"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_236790437.1"
                     /translation="MKEVYGYIRVSTETQAEKGYGLHTQENAIKDYCRQNKLKLVEIFK
                     DEGISGTTIDRNGLSDLLSSLTDIKTVIVMNTSRLWRSDNAKVMIKRQLIKAEVEVLSV
                     EQSTYSIYDKDPNDFLVNGMMELLDQYDRMNTNLKLAKGRRSKAKSGIKGGGNVPLGYQ
                     WQHDGVETPIVIIDESNAQIVRDIFKKYYEYKSIGKVRKYLEEQGYKTARGNSFSQQSI
                     ATILRNPFYKGEIIHADVKTKGQHEPLISAVHFGKIQAMLERNQRNKAK"
                     /name="recombinase family protein"
ORIGIN
        1 tgattgaagt tgatactatt ccagatacag aagatggacg aaagcaagct gactttcttt
       61 ctaagctaaa agaagcggta acaagggaag agcaatcaca tgtgccttac aagagcttag
      121 atgttgttgc agagcgttta gaagacgatt cctacagatg tacgccaaat aaactacttc
      181 aatcatagtg aaacgattat gttcgaaggc agttatgaga aagaaaggtg ttggcgttag
      241 ccttgtcatt ggcgttagcc atctgtttga gcgtagcgag tatggctggc gacggtggca
      301 acggttgctg acacaaagtt tctactcatc acctggagtc gaaggataaa tagtggaacg
      361 aacaaagata gtacgccaaa ggtcttctta tttcagagcg tggatcataa tggcagcagc
      421 catctgcatt tggaggcgtg taccctgccg taggccctct accccagggt accctcggat
      481 gtttgtttgg ggcaatgagt aacgtatacc ttgcactgtg tggcgaggga atttttttgt
      541 cctatatgta aattttttat acaggtgtag gtgatgacgt ctaaaaggct tagattggct
      601 tgtaagcaga cttctgagaa acgttgatat aacaacaatc ctggctatta aagtaccttg
      661 tgggtcgtag gaataggtca aacggcaaac tctgataaga tataaataag ggcttcgtgt
      721 cctcgtcaaa atgttacaga aattattcat gcttccctat gcctcgctaa gatgtgattt
      781 aaagcctctg gaagtctcat atttcgagtt tggagcgagg aaggagcaaa tacatgtgcc
      841 gtgccactag gaggacatat ggctccgcta tgatgtatag aaaataacat ttgtcttttg
      901 taatgatatt gagttaaggt ggattacaag ggactttgag ttgaccctat tctaatagca
      961 ggtcaacggt ctatttttta gactttccac tacaaccatt aaatcttttt gaaaggagta
     1021 actattgcat gacaacaaac agacaactga atatgggtga aatatcctca atccaattgg
     1081 acaggcttac tatagttggt accatacgac gtgataaaga gaattacagt ccgttgttta
     1141 tgaaactact gaatggaata gataagcgtc tgcaagtaga cccgtattac aagaatcatt
     1201 tgtatgaccg taaaattggt gtaagaacag aggaaagtac agccttcatt gaattgagta
     1261 agaataaaac gatgtatgca tctgattttc gtttagattt caatcctact aaatgcaacg
     1321 aggaagatat aaagtacatc aaagagatat tgcattacat cgacaataaa aaggctacta
     1381 ggattgatat agcggcggat ttccttcatc agaacttctc gcaataccat atagaggacg
     1441 gcagacggag aaaaactaat cgtattactg gtgctagtgg tcgtttggag acggaataca
     1501 gaggtgtgag agaatccgat gattatatca aactgtatga caaatcaaga gaacgtttcg
     1561 ataagaaatg ggaaaaggta gactatgagt ggtttaggtt agaagaaact gcaaaaggga
     1621 agaaagcgaa ggattggaca aattatgagt ggttcgaaaa tataagactt gttccagatc
     1681 ataagaatcc tattttccct gaagatgtaa aggcaactac aaaatcacaa gcttattcag
     1741 ttatcatggg gttccaaaac ataacagagt tttcgaaggg gacgcaaacg aagatacgtg
     1801 aagtgataag agaagctaaa tttcacggtg aagtgaatat atacgaacaa ataaaaaaag
     1861 caccgcacca gcaaatgctg aacgatgctc tagaaaaaat aagattcttc ctaaagtgac
     1921 tttccaacgt ctcgcaaacg atgaaagtca ttactatcaa gttagctact gatagtatat
     1981 tacaaccttt atgtataata caataacatc acataacttg tcataaactt acatggctct
     2041 gtcgcttaga cagggctttt tattttgctt tattccgttg atttcgctct aacatagctt
     2101 gaatcttgcc gaagtgtacc gcacttatta acggttcatg ttgtcctttt gtcttcacat
     2161 cagcatgtat tatctcacct ttgtagaaag gattcctcaa gattgtagcg atgctttgtt
     2221 gagagaaact attacctcta gcggtcttgt atccctgttc ttcaaggtac tttcttactt
     2281 tgcctatgga tttgtactca taatactttt taaagatatc tcgtacaatt tgggcgttcg
     2341 actcatctat tatgacaatt ggtgtttcta ctccatcgtg ttgccattga tagcctagcg
     2401 ggacgttgcc cccgcctttt attccacttt ttgctttgga gcgtcttcct tttgctaatt
     2461 taagatttgt attcatacga tcatattggt ctagtagttc catcatgcca tttacaagaa
     2521 agtcatttgg gtctttatcg taaattgaat aggtagattg ttctacgctc aatacttcca
     2581 cttctgcttt aattaattgt cgcttaatca taaccttagc attgtcagaa cgccacaaac
     2641 gagaagtgtt cataacaatg actgttttta tatctgtgag actggaaagc aggtcggata
     2701 atccatttct gtctatggtt gtaccgctta ttccctcgtc tttgaagatt tccactaact
     2761 ttaacttatt ctgcctgcaa tagtccttta ttgcgttctc ctgcgtatgt aatccataac
     2821 ctttttctgc ttgtgtttcg gtactaacac gaatgtatcc atatacttct ttcatgttat
     2881 ctcctcctaa tttcatttta atttaattac tgtcttttat caactctcat tcatgacaat
     2941 tgacaaaaat atatgattaa acgctatata atagcattaa ttacaatttc atctaacgtg
     3001 aagaaaagac aattttatta tcaaaaagta ttgcaccttc atttgctagt ggttataaat
     3061 taattacagt ttcgagattt gatgtagcag tcaatatatt ttgtggtatt agtaaccacg
     3121 ctctccttga gtttttttca tagtgcccac ttcttaggct gagtgggcta cataactact
     3181 actccgaaag gatttgatat agatgagtaa cgcagaagga ctacgtgtat tagtggatat
     3241 agatgactta ccaactcatc aacagataag agtaaaacgt gtagcagagg ggctgacaca
     3301 aactcatctt gttgaattat taggcttacc taatgttcct ttcctctcat acattgagac
     3361 gggggcaaaa cctattccta aatggttgga aacaggcatg aaacgatatc tttatgagga
     3421 ggtctacaaa gatggtcagt tacaaaagaa tgt
//
