LOCUS       NZ_AP022945             5289 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain J7 plasmid pJ7_9, complete sequence.
ACCESSION   NZ_AP022945
VERSION     NZ_AP022945.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203332
            Sequence Read Archive: DRR206372, DRR206396
            Assembly: GCF_021655135.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 344x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655135.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 07:11:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,087
            CDSs (total)                      :: 5,938
            Genes (coding)                    :: 5,644
            CDSs (with protein)               :: 5,644
            Genes (RNA)                       :: 149
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 294
            CDSs (without protein)            :: 294
            Pseudo Genes (ambiguous residues) :: 0 of 294
            Pseudo Genes (frameshifted)       :: 177 of 294
            Pseudo Genes (incomplete)         :: 134 of 294
            Pseudo Genes (internal stop)      :: 43 of 294
            Pseudo Genes (multiple problems)  :: 50 of 294
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022945.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="J7"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pJ7_9"
                     /geo_loc_name="Japan:Tochigi"
                     /collection_date="2006"
     gene            join(5234..5289,1..298)
                     /locus_tag="L6482_RS30235"
     CDS             join(5234..5289,1..298)
                     /locus_tag="L6482_RS30235"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_076868344.1"
                     /translation="MASKNKHKIIEENRDIFLKRTSGMAHDLESVSNILVNFGLAKNRR
                     IAQRWVRENRIKAEWLSGEDDRRAGKIVKESEIFKTITTEIPALKVMFNAIEELQKEND
                     ELKKQIEQSSKQK"
                     /name="hypothetical protein"
     gene            317..658
                     /locus_tag="L6482_RS30240"
                     /old_locus_tag="BCJMU07_p91"
     CDS             317..658
                     /locus_tag="L6482_RS30240"
                     /old_locus_tag="BCJMU07_p91"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2538 family protein"
                     /protein_id="WP_080501341.1"
                     /translation="MYFENATHEQNYNDMMKFYSLAFGENPAFESAIYIAAYSQIHDKI
                     SKSKMDKNANPLAIALESDYLSNSAESLALLGQSLFNGEKVDVSSVVTRGKEIFEIFVQ
                     ACKIRGSLN"
                     /name="DUF2538 family protein"
     gene            1152..1343
                     /locus_tag="L6482_RS30245"
                     /old_locus_tag="BCJMU07_p92"
     CDS             1152..1343
                     /locus_tag="L6482_RS30245"
                     /old_locus_tag="BCJMU07_p92"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_076868343.1"
                     /translation="MQKAKRGRPRMNKTYRQNVVRFSYMEDTIKNIMDLVDSEERDKIN
                     ALLSNLVDHEKQKVAMKS"
                     /name="hypothetical protein"
     gene            1519..4059
                     /locus_tag="L6482_RS30250"
                     /old_locus_tag="BCJMU07_p93"
     CDS             1519..4059
                     /locus_tag="L6482_RS30250"
                     /old_locus_tag="BCJMU07_p93"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_220784067.1"
                     /translation="METHECGKQSVLVQDKATKEMYGFVAQCKRMDCDSCGTRIQTYVQ
                     NKIAMYTKKFDLKHFATITMGDSDIKNLLKKFGSLVDKTRELEKEYFIKRYISRKRSTR
                     EIAEKKYYEYVHSLYDQEHYYRWLYGKDLSKKEKNINYVPISNIGYQITKMITQRPARL
                     QDQIKIILEMKQGKKLRKNQLEKYYKDNVESQRKKWVGWSYADTKKEGRREEFLNDFHV
                     LLTDIAMNMYTQLYPNEEERTIYRSKIPKENTEMAYIRTIEFHNTVDAAHIHSLFNFYI
                     PIKLLKSVMGNHVYDMAEISNLASENLEEKCEEEHDFHQYVARYVSKYITKSSLEVYEK
                     LKEEGEDRVNIISSSDNISIVLEEAFETPYKGKFQLLSVVKGVLPQHYMKFSTIQEFQY
                     FILDSIADEHPNQYIDKTISCFKDYFKEARKKGLSNRDREILKDELKANENELLQNALK
                     LELERRSKEIFVPKQLKLRKTLKQGSLTTEQFEALQGITSSDLFSFLIGYAGTGKTYTL
                     STLLDYIDFEKHDVAVVSYTAQATQRVRSMLKEQNINVDNLEINTIHKLCGAKHSMIDY
                     PIFYNDWSKPLPYSFVIIDEFSLIDIRVLTSFLASLKPTTKVLFVGDPKQLNPIHSKHP
                     YFYLKDFAGVYELTKVLRSGDRIIDVATSVRNGDIEKVPFEVMDFKNSEEKIAEYKKRG
                     YQVLSNSVALKDWINNLFQKDKKQIQSKFNYAQFDNIIFIKNDQYKRYVNGEKGQIIDY
                     NEFGVTIRTENNNEVVLKDYEFFNRVEPNHCLTVHKSQGSEYDKVLVLLDATKTLLLNR
                     NLLYTAITRAKTDVVVMINKVDDKVKTLLKQEQE"
                     /name="AAA family ATPase"
     gene            4296..4559
                     /locus_tag="L6482_RS30255"
                     /old_locus_tag="BCJMU07_p94"
     CDS             4296..4559
                     /locus_tag="L6482_RS30255"
                     /old_locus_tag="BCJMU07_p94"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_220784068.1"
                     /translation="MKERYSVTQKYMVAGVDEKEKVSLYTLIEVPAIKKRMVVCIGNKV
                     DGLVVGNTYEVELDISIETKKFDTKDGVKYVDVASKFVRDIR"
                     /name="hypothetical protein"
     gene            4591..4827
                     /locus_tag="L6482_RS30260"
                     /old_locus_tag="BCJMU07_p95"
     CDS             4591..4827
                     /locus_tag="L6482_RS30260"
                     /old_locus_tag="BCJMU07_p95"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_076868345.1"
                     /translation="MKPNRYGRFRKEDVLKNGYEIVEYGITATEIEKQLGVKLTMKQRL
                     KHCIGFRCEKSWRGCVPVFDILDIALKNDRNQL"
                     /name="hypothetical protein"
     gene            5065..5220
                     /locus_tag="L6482_RS30265"
                     /old_locus_tag="BCJMU07_p96"
     CDS             5065..5220
                     /locus_tag="L6482_RS30265"
                     /old_locus_tag="BCJMU07_p96"
                     /inference="COORDINATES: protein motif:HMM:NF024513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3970 family protein"
                     /protein_id="WP_175556117.1"
                     /translation="MIKIRLQGLSQEMQEMIEDLKKHYDILSISDFYQNRNSQYIRCYI
                     EMRLKS"
                     /name="DUF3970 family protein"
ORIGIN
        1 aagaacaagt ggcatggcac atgatttaga atcagtttca aatattttag tgaattttgg
       61 actagctaaa aatcgtagga ttgcacaacg ttgggtgcga gaaaatcgta ttaaagccga
      121 atggctaagt ggcgaggatg atcgccgagc agggaaaatt gttaaagaga gtgagatatt
      181 caaaacaatt acaactgaga ttccagcgtt gaaggtaatg ttcaatgcca ttgaggaatt
      241 acaaaaagaa aatgatgagt taaaaaagca aatcgaacaa tcgagcaaac aaaaataaga
      301 aaataggggt gtttgtatgt actttgaaaa tgcaactcat gagcaaaatt ataatgatat
      361 gatgaaattt tattcactag cttttggtga aaatccagca tttgagagtg cgatttatat
      421 cgcagcttat tcgcaaattc atgataaaat ttctaaaagt aaaatggaca aaaatgcgaa
      481 tccattggca attgcattgg aatctgatta cttatcaaat tcagcagaat cattggcatt
      541 attggggcaa tcgcttttca atggggaaaa agtagatgtt tcttctgttg ttacaagagg
      601 taaagaaatc tttgaaattt ttgttcaagc ttgtaaaatc cgtggtagtt tgaattaaca
      661 cttagtaata aactaagtgt tttttactaa aatataatat atacaatgta taaggttgaa
      721 ctttgaggga attggtataa ttaaaaagtc taaagtttaa cattgaactt tgttaaaata
      781 catagaagaa gggttttcga gactttttgg gtagaggtac tggttcgtgg tttgctgacg
      841 agtcagcaag gttttgcttt tgccgagcgt ttttgggacg ctacaatagg caaacaagac
      901 gaaaacaaat tagtgagctt tttggtatgt cggcttggtg catgacaaaa agcgttccaa
      961 aatggtactt gtcaaatgat gtttaggtgg agatttttgc atggtcttcg caaaaatact
     1021 acctatcatt tgacgaggaa caagcggatt ccctatgaaa gtaaaaattt ttgcaaaaaa
     1081 aactaggaat tttgcaattt tgcgctaaac tatttattgt ataacaaaaa aatagaaagg
     1141 aggtgtgata gatgcagaaa gccaaaagag gaagaccaag aatgaataaa acatatcgtc
     1201 aaaacgtagt tcgtttctcg tacatggaag atacaataaa aaacattatg gatttagtag
     1261 attcagaaga acgtgataag atcaatgctt tattaagtaa tttagtagac catgagaaac
     1321 aaaaagttgc aatgaaaagt tgaggtgagc gacttttccc ttaacttgat tacagtaatg
     1381 gcgaaaatac ccgcacaaaa gtcactttct caattagaaa acagctatga gccttacagc
     1441 ccgttgggtt tcggggcaaa aaagccatgt acgttttttt tcattctcaa ttagacaaca
     1501 agaagggagg gagagtcgat ggaaacgcat gaatgtggga aacagagtgt tttggtacag
     1561 gataaagcaa caaaggagat gtatggtttt gttgcacaat gcaagagaat ggattgtgat
     1621 tcttgtggaa caagaataca aacatatgta caaaataaaa ttgcaatgta caccaaaaaa
     1681 tttgatttga aacattttgc aacaatcaca atgggtgatt cagacataaa aaatcttttg
     1741 aagaaatttg gaagtttggt tgataaaaca agagaattag aaaaagaata ttttatcaaa
     1801 cgttatataa gtcgaaaacg ctcaacaaga gaaattgcag agaaaaagta ttatgaatat
     1861 gtccattctc tttatgatca agaacattat tatcgatggt tatatggaaa agatttgagt
     1921 aaaaaagaga aaaatataaa ttatgttcca atcagtaaca ttggttatca aatcacaaaa
     1981 atgataacgc aacgtccagc aagacttcaa gaccaaatta aaataatttt ggaaatgaag
     2041 cagggcaaaa aactccgaaa aaaccaatta gaaaaatatt ataaagacaa tgttgaaagc
     2101 caaagaaaaa agtgggttgg atggagttat gcagatacta aaaaagaagg aagacgtgaa
     2161 gaatttttga atgattttca tgttttgctt actgatattg caatgaatat gtacacacaa
     2221 ttgtatccaa atgaagaaga aagaacgatt tatcgttcga aaattccaaa ggaaaataca
     2281 gaaatggcgt acattcgaac gattgaattt cacaatactg ttgacgcagc acacattcat
     2341 tctcttttta atttctatat cccaattaaa ttattgaaaa gtgtcatggg aaatcatgtg
     2401 tatgacatgg ctgaaattag taatttagca agtgaaaatt tggaagagaa atgtgaagaa
     2461 gaacatgatt ttcatcaata tgttgctaga tatgtatcaa agtacattac aaaatctagt
     2521 ttagaagttt atgagaaatt gaaagaagaa ggcgaagacc gtgtgaacat tatatcttcc
     2581 agtgataata tttcaattgt gttggaagaa gctttcgaaa ctccatataa agggaaattt
     2641 caattgttga gtgttgtaaa aggtgtgttg ccacaacatt atatgaagtt ttcaacgatt
     2701 caagaatttc aatattttat tcttgatagc atagcagatg aacacccaaa tcaatatatt
     2761 gataaaacaa tttcttgttt taaagattac ttcaaagaag caaggaaaaa gggattatca
     2821 aatagagata gagaaatatt aaaagatgaa ttgaaggcaa atgaaaatga attgttacaa
     2881 aatgctttga agttagaact tgaaagaaga agtaaagaaa tttttgtacc aaaacaattg
     2941 aagttgcgaa aaacattgaa acagggttca ttgacaacag aacaatttga agccttacaa
     3001 gggattactt caagtgattt attttcattt ttgattggat atgcaggaac aggaaaaact
     3061 tatacactat caacattgtt ggattatata gactttgaaa aacatgatgt agctgttgta
     3121 agctatacag cacaagcaac acaaagagtt agaagcatgt tgaaagaaca aaatattaat
     3181 gttgataatt tggaaataaa cacaatacac aaactctgtg gggcaaaaca ttcaatgatt
     3241 gattatccaa tattttataa tgactggagc aaaccattgc catattcatt tgtaatcatt
     3301 gatgagttct cattgattga tataagagtg ttaacttcat ttttggcttc attgaaacca
     3361 acaacaaaag tattgtttgt aggtgatcca aagcaactca atccaataca ttcaaaacac
     3421 ccatactttt atttaaagga ttttgctggt gtttatgaat taacaaaagt attgcgaagc
     3481 ggtgacagaa tcattgatgt agcaacttct gtgaggaatg gagatataga aaaagttcca
     3541 tttgaagtga tggattttaa aaacagcgaa gaaaaaattg ctgaatataa aaaaagaggc
     3601 taccaagttc tatcgaactc cgtagcgtta aaagactgga taaacaattt atttcaaaaa
     3661 gataaaaaac aaattcaatc aaaatttaat tatgctcaat ttgataatat catatttatc
     3721 aaaaatgacc aatataaacg ttatgtgaat ggtgaaaaag ggcaaattat tgattacaat
     3781 gaatttggcg taacaattag aacggaaaac aataatgagg ttgttctaaa agattatgaa
     3841 ttctttaata gagtagagcc aaatcattgc ttaactgttc ataaatcaca aggttcagag
     3901 tatgacaaag tattggtatt actggatgca accaaaacat tattactcaa tagaaaccta
     3961 ttgtatacag cgatcaccag ggcaaaaact gatgttgttg taatgataaa caaagtggat
     4021 gacaaggtaa aaacattgtt aaaacaagaa caagagtaaa tttattttat cttatattaa
     4081 tttctataaa aggcaaggga aaccttgtct ttttctgagt aaaaaaatat aaaataaaaa
     4141 aaggataaaa taaaataaaa ttaaaataaa taaatgtaaa acgtataaag ttcaactttg
     4201 tatgttatat aaaggaaaaa gaaagaaaaa cttgacaaga agaaaaatgt atttataatt
     4261 taacaaattt atattaaaaa aggggtgctt aaaacatgaa agaacgttat tcagttacac
     4321 aaaagtatat ggttgcaggt gttgatgaga aagagaaagt ttcactttat acattgattg
     4381 aagtaccagc aattaagaaa cgtatggtag tttgtattgg taataaagtt gatggattag
     4441 tagtagggaa tacatatgag gttgagttag atatttcaat tgaaacaaag aagtttgata
     4501 caaaagatgg agtaaaatat gtagatgttg ctagtaagtt tgttcgtgat attcgctaat
     4561 ttatgaattt tcatggtgga ggtggttcaa ttgaaaccta atcgttatgg gcgattccgc
     4621 aaggaagatg ttttgaagaa tggttatgaa atcgtggaat atggcataac agcaacggaa
     4681 attgagaagc aattaggcgt aaaattgacc atgaagcaac gattgaaaca ttgtatagga
     4741 ttccgttgtg agaaatcatg gcgtggctgt gtgcctgtct ttgacattct agatatagcg
     4801 ttgaagaatg atagaaatca attataaaac acatgtaagc ttaccattaa atttgcgtag
     4861 cgttgctaga ggtggggtgc tggggtgtca ctggattact gtgccgtgct tgggaagcac
     4921 agctatacag ctaaaaagac acacctacac cgtaccactc acaaccatca tgccttgcat
     4981 gatacatata taaataaaat ataaagttta aggttgaact ttgtatgact ttgttgtaaa
     5041 atggaattat gggaggaatt gaatatgatc aaaattcgtt tgcaaggttt atctcaagaa
     5101 atgcaggaaa tgattgagga cttgaagaag cattatgaca tattatccat ttctgatttc
     5161 taccaaaacc gcaatagcca atacatacga tgttatatag aaatgcgttt aaaatcatag
     5221 ggaggttgtt attatggcat caaaaaataa gcacaaaatc attgaagaga atcgtgacat
     5281 tttcttaaa
//
