LOCUS       NZ_AP022943             9261 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain J7 plasmid pJ7_7, complete sequence.
ACCESSION   NZ_AP022943
VERSION     NZ_AP022943.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203332
            Sequence Read Archive: DRR206372, DRR206396
            Assembly: GCF_021655135.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9261)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 344x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655135.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 07:11:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,087
            CDSs (total)                      :: 5,938
            Genes (coding)                    :: 5,644
            CDSs (with protein)               :: 5,644
            Genes (RNA)                       :: 149
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 294
            CDSs (without protein)            :: 294
            Pseudo Genes (ambiguous residues) :: 0 of 294
            Pseudo Genes (frameshifted)       :: 177 of 294
            Pseudo Genes (incomplete)         :: 134 of 294
            Pseudo Genes (internal stop)      :: 43 of 294
            Pseudo Genes (multiple problems)  :: 50 of 294
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022943.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9261
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="J7"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pJ7_7"
                     /geo_loc_name="Japan:Tochigi"
                     /collection_date="2006"
     gene            complement(233..370)
                     /locus_tag="L6482_RS30150"
     CDS             complement(233..370)
                     /locus_tag="L6482_RS30150"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236802744.1"
                     /translation="MDNELYHLLLREICLEKMRVLIQDLERIQALLEQMLTEDMSRKPE
                     "
                     /name="hypothetical protein"
     gene            complement(774..896)
                     /locus_tag="L6482_RS30410"
                     /old_locus_tag="BCJMU07_p71"
     CDS             complement(774..896)
                     /locus_tag="L6482_RS30410"
                     /old_locus_tag="BCJMU07_p71"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272884805.1"
                     /translation="MDKDNLYEEMKKKDKAFDEKIKEMDEEIEELKDKLLGEKE"
                     /name="hypothetical protein"
     gene            complement(1070..1387)
                     /locus_tag="L6482_RS30155"
                     /old_locus_tag="BCJMU07_p72"
     CDS             complement(1070..1387)
                     /locus_tag="L6482_RS30155"
                     /old_locus_tag="BCJMU07_p72"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236802746.1"
                     /translation="MAKTYSMQEMRQEKEVLQRLYDVFVKELDARLENNDGLVTMAQMN
                     YCIDRAMSSERLEKEQKKVANSFFDTFVGAWFTSGKQMAESMAFAVTTDTVDDETRFHF
                     L"
                     /name="hypothetical protein"
     gene            complement(1399..1608)
                     /locus_tag="L6482_RS30160"
                     /old_locus_tag="BCJMU07_p73"
     CDS             complement(1399..1608)
                     /locus_tag="L6482_RS30160"
                     /old_locus_tag="BCJMU07_p73"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236802748.1"
                     /translation="MDEKLEKVMDYIDETHDLTISMMSAMQILVEQTKDIELHDLLFVL
                     TCFRDNAKHDDVYEFLNKVLTNKE"
                     /name="hypothetical protein"
     gene            complement(1795..3246)
                     /locus_tag="L6482_RS30165"
                     /old_locus_tag="BCJMU07_p74"
     CDS             complement(1795..3246)
                     /locus_tag="L6482_RS30165"
                     /old_locus_tag="BCJMU07_p74"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="WP_236802750.1"
                     /translation="MGLIQESKLFYIEPTSFGDVGYQEKYLGLSLKAVNQRDFFEDVFG
                     DFSGYMCVFQKRSNGTTANTFFKKNDEEKMIRFLKKTFGQDTYFSYSTYFSKDRKKKRT
                     QDNIVHTYMIVQDLDYYKFGMSDKEFLEKLGEMIRRGGIICPSYIISTGQGYQLIWLVK
                     PFKNIAGYTNDKDWHTIQEFLYQKLKEFNSDSVVKNPSAVTRLVGSKHRKTGNLVYGYK
                     ANEVRLDLKDFMFYYNLVPTPDRLVKPKKKPSTSKKTTTKTSVTRMVANWNEFTLNRYR
                     EEDIFIFVQEQNERGVTYIGIRNWLALVLRFHSLVSTNGDTKYAKERVLQLCSIMDMTE
                     TTVEEILRRSEPSERYYDEWVNDTWDKNKYMRGGLFYTNARMLDLMNIKEDYEMQWKMK
                     TIKIVNKKYTAARKRFEKFGVKEADNHTWEAYQERRNEEIEVKKDDKLEQLKEAIKKHP
                     EASQRKLAKILGWSVGTVNNYKNKL"
                     /name="winged helix-turn-helix domain-containing protein"
     gene            complement(3610..4323)
                     /locus_tag="L6482_RS30170"
                     /old_locus_tag="BCJMU07_p75"
     CDS             complement(3610..4323)
                     /locus_tag="L6482_RS30170"
                     /old_locus_tag="BCJMU07_p75"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_236802752.1"
                     /translation="MKKAVCYYRKSIEREAEKSIKGQQEEVIRYAKENGIEIVEEFHEV
                     ASSMTTDRKELNRMFQYLEEHKDIDYVLVHRFDRMTRDMLGLGYVMEMLKKGKTRLHSA
                     TEDNDYENDPFKVLLIVMKTFGSEVEKNNIVERMREGRERKQRNGGFIGGTPPIGYRSV
                     VGTGMLEVEEKEVPIVQDVFSLKEKGLTLQQIADILNEKGYETRRKKRFHPPLVRRILL
                     YRDWYEGKAQSPRIL"
                     /name="recombinase family protein"
     gene            complement(4471..4632)
                     /locus_tag="L6482_RS30175"
                     /old_locus_tag="BCJMU07_p76"
     CDS             complement(4471..4632)
                     /locus_tag="L6482_RS30175"
                     /old_locus_tag="BCJMU07_p76"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236802755.1"
                     /translation="MKIETDAFLEDFEWRLSDEHLSELPFEEVANKVIPKLKREWGNGK
                     GKKVKGLR"
                     /name="hypothetical protein"
     gene            complement(4638..5237)
                     /locus_tag="L6482_RS30180"
                     /old_locus_tag="BCJMU07_p77"
     CDS             complement(4638..5237)
                     /locus_tag="L6482_RS30180"
                     /old_locus_tag="BCJMU07_p77"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236802738.1"
                     /translation="MRNDLFDTYYIYTSKEMAEKINIKTPTVRKYGQLLEKYGYKFEKN
                     GERRNFTDKDVIVMQKMKESTNIERTAKELADLQRNGIDINDIKPDMTRNDINISPSDT
                     GLIVREFQQKMADSEKRYGEMMNQVLGELKENQMIMQQTTQELADLRHREAEKQKTIDD
                     MSEKLSKAVDLLLEMEKNQEKNKKKSIFEMIKNISK"
                     /name="hypothetical protein"
     gene            complement(5459..6634)
                     /locus_tag="L6482_RS30185"
                     /old_locus_tag="BCJMU07_p78"
     CDS             complement(5459..6634)
                     /locus_tag="L6482_RS30185"
                     /old_locus_tag="BCJMU07_p78"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236802739.1"
                     /translation="MNFKKSDKVWEWLMKSGIGFSKGKIERGSGLARSAVMKYKTDFNR
                     LMKMVEKETGKNDPMKVKPNDVYKVIDKLIKEYHAGNKAHANFLRGVDDALHAFKECSK
                     ASGVYKRELRLGDKRVISQKLNDEKVYRRSKDTTVMKASRDDMEKVVAQIEKSRASREV
                     KDVAINVVRLEFESGRRISGILRSQVGNYNEKNGTYTTYGDKGGKDNESFYLTNGAKEI
                     LNKLSRNEDGEKKKDGDMMFKVKYTQHKDPNKRGQDKSVDNIRKQVSNLIKNCAERAGV
                     NRDGQKFSSHSCRKGYANERADHYIDNMTAEERKEELERRKKQDKNLAERIDKCLEHIK
                     SKFEIEKNKEKREFTDIEIIKLLVSTDINHSRLDVMRYYLMDYQFDFDSND"
                     /name="hypothetical protein"
     gene            7980..9257
                     /locus_tag="L6482_RS30190"
                     /old_locus_tag="BCJMU07_p79"
     CDS             7980..9257
                     /locus_tag="L6482_RS30190"
                     /old_locus_tag="BCJMU07_p79"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236802742.1"
                     /translation="MNLQEFREKELYPLLEIKRVPVTNTVFLRKIRFPKDVLAPLGTFN
                     TNYYEELKQREEDKNINAYIIDGNILHEQLKTNSLSFYVFHPKKRDVVEGYGGKYIDVH
                     VDFKGFGLYHIVAETGLKVRKKRGDRRFLEVDKDAGIEKEEIYLYYDEEEKQYKRTNKK
                     TFENMLRDINEFKFSDEIAKMIYDYKTFFSELFLNYTAFQSQAPLDSTTTPNQYIQYTA
                     TLQNQISSMEEYYDYYLQIGGISKESHERISQQISDINAHSKALQNYVYMTFTNLDEKI
                     GEPLDEGLRTHYMKLKRTLDEVTSDKYWINTFTGEFTHNMKTFHLSQVPTEFFEDEDLE
                     QKRKLSTLLDIIFHHYINVRKINIENGTHNESGIFYDALTKETNSDNLDKNIEERLEKI
                     RSIYDTQSFTSFPEIENLGYLFESNE"
                     /name="hypothetical protein"
ORIGIN
        1 aaacaaaaaa gagcggtttg gaatcccaaa tcactctttt tttatatttt catgctctta
       61 tttactttca tttcgtttta ttttctttta tgttttgtgc cttgccccgt gcggtatttc
      121 aatttgccct aaatagacgg gaacccctga tacataaagg ctaagaccgt gttcccccga
      181 aaaaaatcct tcggggaaac cctgtctagt ttatatccgt atcctacccc atctattcag
      241 gttttctcga catatcctcc gttaacattt gttctagcaa tgcttgtatt cgctccaaat
      301 cttgaatcaa aacacgcatc ttttccaaac atatttctct caacaacaaa tggtacaatt
      361 cattatccat acaaaccccg cctttccccc acaacttaac agagaatctt ataaatgcaa
      421 tttttcaaca caaaaaagag aagaacaacg ttcttcccat tcctcacttt aggatattta
      481 tttggatact cataatgaaa aaaagacacg agacttaata ttttcatcct tactacagga
      541 tatttgtgta cctaaatcga cctaacttcg gtgcaagttc cttattggca tctttgctat
      601 aagatatttg tgaacgaggc tcgtctactg aatttaaagc cgttaacata tttacatctt
      661 tactgtaaga tatttttgca tatttgtttg tctataccat atacactgac atattttcat
      721 ctttatttca agttatctat ccttattatc cccgtattgt ctgtaaatat gcactattct
      781 ttttccccta atagtttatc cttcagttct tctatttctt catccatttc tttgattttc
      841 tcatcaaatg ctttgtcttt ctttttcatt tcttcataca agttatcttt atccataaaa
      901 aaagacctcc tacaacgaat atacattcat catagacaag tctttttcca cctatacgtc
      961 catatctgga aatggtccac taatggagat ggatagcact aagtgaagta caagcgtgga
     1021 caagaggtat catccttata agaatcgtat ttccgaagtg gaaacagaat tatagaaaat
     1081 ggaatcttgt ttcatcatct actgtatcag ttgtaaccgc aaatgccatg ctttccgcca
     1141 tttgttttcc acttgtaaac catgcaccta caaatgtatc aaaaaatgaa tttgcgacct
     1201 tcttttgttc cttttccaat cgctcagaac tcattgctct atcaatacag taattcattt
     1261 gagccattgt taccaatcca tcattatttt ctaatcttgc atctaattct ttaacaaata
     1321 catcatacaa tcgttgcaac acttcttttt cttgtctcat ttcttgcata ctatatgttt
     1381 tcgccatgtt ttcatccctt attctttatt tgtaagcact ttgtttaaaa attcatatac
     1441 atcatcatgt ttcgcattgt ccctaaaaca cgttaataca aataataaat catgtaattc
     1501 aatatctttt gtttgttcta ctaaaatttg catggcagac atcatagata tcgtcaaatc
     1561 atgtgtttca tctatgtaat ccattacttt ttctaatttt tcatccatca aaattccccc
     1621 tctatatcag cacgtatata cttctaacat actgtacaaa aagtgattct cctagattcc
     1681 gtattctttt tctttaatat tggaggtttg gaacttcatc ttttatgaag ttccccatac
     1741 ccctctatcc ttacttcgtc ttttaatggg aaagttgatt gaacacctaa aattttacaa
     1801 tttgttttta tagttattta ctgttccaac actccatcct agaatcttag ctaatttcct
     1861 ttgcgaagct tcaggatgct ttttaatagc ttcttttaat tgttctaatt tatcatcttt
     1921 ctttacttct atttcttcgt ttctacgctc ttgataagct tcccacgtat gattgtccgc
     1981 ttcttttacc ccgaatttct caaaacgctt acgtgctgcg gtgtactttt tgttaacaat
     2041 tttgattgtt ttcatcttcc attgcatttc ataatcttct ttgatattca tcaaatcaag
     2101 cattctcgca ttggtgtaaa acagaccacc acgcatgtat ttattcttat cccatgtgtc
     2161 gttaacccat tcatcataat atctttcact aggttcagaa cgtctcagaa tctcttctac
     2221 agtcgtttca gtcatatcca taatcgaaca tagctgaagc actcgttcct ttgcatattt
     2281 cgtatctccg tttgttgaaa ctaatgaatg aaaacgaagc actaacgcca accaattgcg
     2341 aataccaata taagtaaccc cacgttcatt ttgttcttgt acaaagataa aaatatcttc
     2401 ttctcgataa cgatttaacg tgaattcatt ccaattcgct accatacgag taacagatgt
     2461 ttttgttgtt gtttttttac ttgtacttgg ctttttcttt ggttttacaa gtctatcagg
     2521 cgttggaaca aggttatagt aaaacatgaa atcttttaaa tctaaacgaa cttcgttcgc
     2581 cttgtatcca tatactaaat tccctgtttt tcgatgctta ctacctacaa gacgtgtcac
     2641 tgctgaagga ttttttacca cactatcact attaaattct tttaattttt gatacaagaa
     2701 ttcttgaatc gtatgccaat ctttatcatt cgtatatccc gcaatgtttt taaatggttt
     2761 cactaaccaa atcaattgat atccttgccc cgtagaaatg atgtaacttg gacaaataat
     2821 tccccccctg cgaatcattt cacctagttt ttctaagaat tctttatcag acataccgaa
     2881 tttatagtaa tctaaatcct gtacaatcat atatgtatga acgatgttat cttgcgttct
     2941 tttcttcttt ctatcttttg aaaagtaggt agaatacgag aaatatgtat cctgtccaaa
     3001 cgttttcttc aagaaacgaa tcattttttc ttcatcattt ttcttaaaga atgtatttgc
     3061 tgtcgttcca ttggaacgct tttgaaatac acacatatac cccgaaaaat caccaaatac
     3121 atcttcgaaa aaatctcttt gatttaccgc ttttaatgat agtcctagat atttttcttg
     3181 atatcctaca tctccgaaac tcgttggttc tatataaaat aacttacttt cttgaattaa
     3241 tcccataaaa atatccctcc cttggaaaat ggcgtttttt aggagagatt ttcatcatta
     3301 ttaaaaggaa ttgtcgtttg tatatccaat taaaatctaa gaacgtttca ttgacaaaac
     3361 caatgttagt cttatattat aattatataa atttaaaacg acattttctc tccaattctt
     3421 tttaattgga tgatgagaat ctctacgaga gaagttagtg aagtccgcca agacaataat
     3481 cactacttct tttttttgtt tctaaaacaa attttaacat tcttctattc attttcctat
     3541 ctttctatct actttattac atttttagcc cttataccct ctatctagca ataacgcaac
     3601 caaaattccc tataaaatac gaggtgattg tgccttacct tcataccaat cacgatacaa
     3661 aagaatcctt cttactaaag gtgggtggaa tcgtttcttt ctacgtgttt catagccttt
     3721 ttcatttaat atatcagcta tttgttgcaa agtaagtcct ttttctttta atgagaacac
     3781 atcttgtacg attggaactt ctttttcttc tacttctagc atccctgtcc caacaacaga
     3841 acgatatcca attggaggtg tacccccgat aaacccgcca tttcgttgtt ttcgttccct
     3901 gccttctctc attcgctcca ctatgttatt cttctctacc tcagaaccaa acgtcttcat
     3961 gacgatgaga agtaccttaa acgggtcgtt ttcatagtca ttatcttccg ttgcactatg
     4021 aagtcttgtc tttcctttct ttaacatctc catcacataa cccaggccca gcatatccct
     4081 tgtcatgcgg tcaaaacgat gtactagcac gtaatctatg tccttatgtt cttctaagta
     4141 ttgaaacatt cgatttaact cttttcggtc tgttgtcata gacgaagcca cctcatgaaa
     4201 ttcttctacg atttcaatgc cgttttcttt tgcatacctt atcacttctt cttgttgccc
     4261 tttaatggat ttctctgctt ctcgttcaat tgattttcga taataacaaa ctgctttctt
     4321 cataatcagt atacctccta tgtacctact tttcattcgc tccgatgaat agaatatttt
     4381 cttttaaaaa aactgccttg cggtcatttg catttcagtg gggcgaaagt cttgccccac
     4441 caaaacacaa aggtatatta gtctacttta ttatcgtaga ccttttactt tcttgccctt
     4501 gccatttccc cattctctct ttaattttgg tattacttta tttgccactt cttcgaatgg
     4561 taactcagaa agatgttcgt cacttaatct ccattcaaaa tcttcaagaa acgcatcagt
     4621 ttctattttc acaccattta ttttgatata tttttaatca tctcaaaaat gctttttttc
     4681 ttgttttttt cttggttttt ctccatttcc aatagcaaat cgactgcttt gcttagtttt
     4741 tcgctcatat catcaatggt tttttgtttt tccgcttctc tatgccttaa atcagctaat
     4801 tcttgtgttg tttgttgcat aatcatttga ttttctttta attctcccaa aacctgattc
     4861 atcatttcac catatcgctt ctcagaatcc gccatttttt gctggaattc tcgaactatc
     4921 agtcccgtat cgctaggcga tatgtttata tcgttcctcg tcatatcggg ttttatatcg
     4981 tttatatcga ttccattccg ttgcaaatca gccaattctt ttgcggttcg ttcaatattg
     5041 gtactttctt tcattttttg cataacaatt acatctttat ccgtgaaatt tcttctttca
     5101 ccgttctttt caaacttata accgtatttt tctaacagtt gcccgtattt cctcaccgtt
     5161 ggtgttttaa tattaatctt ctcagccatc tctttcgatg tatatatgta atatgtatcg
     5221 aataaatcgt ttctcatatc atctcacctc tcttagagtc ttctatacta tcagtttctt
     5281 cccctttacc taagtataaa cgatataaaa aaacagagga attttcccct gtcttttgtt
     5341 tggatgatgc tttttgcatc tatccaatat gaaaatatag tgttttgaag cttgtttttt
     5401 agttgtattc actatttata tatgtttttt gatattttac aagtaaatta ctgcgatttt
     5461 aatcatttga atcaaaatca aactgataat ccattagata ataacgcata acgtccaatc
     5521 ttgaatggtt tatgtcagtg gacacaagca atttaatgat ttcaatgtcc gtaaattctc
     5581 gtttttcctt gtttttttca atttcgaact tacttttgat atgctccaaa catttatcga
     5641 tacgttcagc taggttttta tcttgttttt ttcgtctttc tagttcttct tttcgttctt
     5701 ctgccgtcat gttatcgatg taatggtccg ctcgctcgtt cgcataccct ttgcgacaac
     5761 tatgactaga gaatttttgc ccgtctcgat tcacgccagc tctttccgca caatttttaa
     5821 ttaaattact gacctgtttt cgtatattat caaccgattt atcctgtcca cgtttatttg
     5881 ggtctttatg ttgtgtatat ttgactttaa acatcatatc tccatctttt ttcttttctc
     5941 catcttcatt tcgtgataat ttatttaaaa tttctttcgc tccattcgtc aaatagaaac
     6001 tttcattatc ttttccgcct ttgtccccat atgttgtata tgttccgttt ttttcattgt
     6061 aatttcctac ttgagaacgc aaaatccctg atattcttcg ccctgattca aattctaatc
     6121 gtaccacgtt aattgcaaca tctttcactt ctctcgaagc tcttgatttt tcaatttgag
     6181 ccacgacttt ttccatatca tcacggctgg ccttcatcac agtggtgtct ttggaacgtc
     6241 tatacacctt ctcatcgttt aatttttgcg aaatgactcg tttatcgcct agtcgaagtt
     6301 ctcttttata tacaccagaa gccttcgaac attctttgaa agcgtgtaaa gcatcatcta
     6361 cacctcgtaa aaagttcgca tgtgctttgt ttcctgcgtg gtattccttg attaatttat
     6421 caatgacttt atatacatca tttggtttta ctttcattgg gtcgttcttt cctgtttctt
     6481 tttccaccat tttcattaaa cgattaaaat cggttttata tttcataacg gcacttcttg
     6541 ccaaacctga acctcgttca attttgcctt tcgaaaaccc aatacctgat ttcattaacc
     6601 attcccaaac cttatcagac ttcttaaaat tcacaaacat caccccctaa aagaagaaaa
     6661 cataaaagat taataccaaa agagaaaaag agaaaagaat aaacatgaaa gagaaaagat
     6721 gaagaacaaa aagatgaaag aagataaagt tcttgcaaaa aaattgcata caaaagccaa
     6781 gtccgttcat ttcccattcg aagaatggtt ctgtcagtgt tacgtttatg ttatctcgtc
     6841 atttgttccg ctcccgcgta acaactgcca agctaacaac acttgttctg cagccgtaac
     6901 aaccgccgag cctcccgtct cggcggtcgt tccgacctcc gaacaaacaa ctcgacagaa
     6961 ccttcttctc aaaaatcaag ttcaaaacct tcaccccaca tcccataaat gggatgtggc
     7021 tccgtaagaa tatcatccgt aacgtggatg aaacgcctcc gattgttccc tgccattctc
     7081 tccatctatc aaaattttat tcccataaaa ttctgataga cgaagagaat tcgagtgtag
     7141 gaacgctcag tgttgcgtac gccaactcaa gtttcgagtc gactacgaca tatgctagca
     7201 ccatcgcata tgaagtccct ttattttcgg agatgggtac acctcacaga gtatgggaat
     7261 gtttatagac gcccaggcag tcttcttacg gatattacaa ccaaaaactt aagttactca
     7321 gttacaacaa aacctaaaaa acaaaattac tcagcttcaa aaaaccataa aacatataca
     7381 acttacttca caaacacctc cacgaaattt tcattatcct ccttcacaac taaaaatagt
     7441 catcttcaaa acctccattt tcatattttt tttggtatac aacttcaaca acttaaaacg
     7501 tttctttttc ctttctacaa caactaaaaa cgcttcttac tacttattac tctccctaac
     7561 aatgacaaaa gacagttagt gtgacctttt tcttctaact gtttgtaaca atgttgctct
     7621 ttgtgtacct atagtctatg tgcatagttg ggagtaaaga actctcagat aggtaaataa
     7681 cgtatccgaa atagtatttt attgtctttg attatccttt ctttttttat ttttttattt
     7741 gaaaccagcg aaatgccgat tcatgccaaa gggcgtaatt tgaatatata ctttaatttt
     7801 actggaaaaa cctgtaaaaa caagtaaaat tctgatataa caatataggt taatggtata
     7861 gattccgacc atttaaaaaa cacagtcata tcaagtgttt gaccgctttt gataaaaatg
     7921 aaaaatcaaa aaaacatagg gtttgtacct tatgaaaatt aaaaaagtga ggtgatatta
     7981 tgaatttaca agaatttaga gagaaagaat tgtatccatt attagagata aagcgagttc
     8041 ctgtaaccaa caccgttttt cttcgaaaaa tacgattccc aaaagatgta ctagcaccac
     8101 taggaacatt taacaccaat tattatgagg aactaaaaca aagagaagaa gataaaaaca
     8161 ttaatgcata catcattgat ggcaatatcc ttcatgaaca actgaaaaca aacagtcttt
     8221 ctttttatgt ttttcatccc aaaaaaagag atgtagtgga agggtatgga ggaaaatata
     8281 tagatgttca cgtggatttt aaaggctttg gactgtatca cattgttgct gaaacaggat
     8341 taaaagtacg taaaaagcga ggtgacagac gattcttaga agtggataaa gatgctggaa
     8401 tcgaaaaaga agaaatttat ttgtattacg atgaagaaga aaaacagtac aaaagaacaa
     8461 ataaaaaaac atttgagaac atgctgcggg atataaatga gtttaaattc agtgatgaaa
     8521 tcgccaaaat gatttacgac tacaaaacat tttttagtga attattcctg aattatactg
     8581 catttcaaag ccaagcaccg ttagattcca ccaccacgcc aaaccaatat atccaataca
     8641 ctgccacact tcaaaaccaa atcagtagta tggaagaata ctatgattat tacctgcaaa
     8701 ttggcggaat ttcgaaggaa tcccatgagc gaatcagcca acaaataagc gatataaacg
     8761 ctcattctaa agcacttcaa aactatgtgt atatgacgtt tacgaaccta gatgaaaaga
     8821 taggagaacc gcttgatgaa ggtcttagaa cgcattatat gaagctaaaa cgcacattag
     8881 atgaagttac atccgataag tattggataa atacattcac aggcgaattt actcacaata
     8941 tgaaaacatt tcatttaagc caagtaccaa cagaattctt tgaagacgaa gatttggaac
     9001 aaaaaagaaa actgtccacg cttcttgata tcatctttca tcattacatc aacgtccgaa
     9061 aaataaatat cgaaaatgga acgcataatg aatcaggaat cttttatgat gcacttacga
     9121 aagaaaccaa tagtgataat cttgataaaa atatagaaga acgtctagaa aagattcgaa
     9181 gcatttacga tacccaatcc ttcacgagct tccctgaaat tgaaaatttg ggctatttat
     9241 ttgagtcgaa tgaatagcaa c
//
