LOCUS       NZ_AP022942            10230 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain J7 plasmid pJ7_6, complete sequence.
ACCESSION   NZ_AP022942
VERSION     NZ_AP022942.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203332
            Sequence Read Archive: DRR206372, DRR206396
            Assembly: GCF_021655135.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10230)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 344x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655135.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 07:11:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,087
            CDSs (total)                      :: 5,938
            Genes (coding)                    :: 5,644
            CDSs (with protein)               :: 5,644
            Genes (RNA)                       :: 149
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 294
            CDSs (without protein)            :: 294
            Pseudo Genes (ambiguous residues) :: 0 of 294
            Pseudo Genes (frameshifted)       :: 177 of 294
            Pseudo Genes (incomplete)         :: 134 of 294
            Pseudo Genes (internal stop)      :: 43 of 294
            Pseudo Genes (multiple problems)  :: 50 of 294
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022942.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10230
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="J7"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pJ7_6"
                     /geo_loc_name="Japan:Tochigi"
                     /collection_date="2006"
     gene            complement(join(7530..10230,1..281))
                     /locus_tag="L6482_RS30115"
     CDS             complement(join(7530..10230,1..281))
                     /locus_tag="L6482_RS30115"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_236802734.1"
                     /translation="MKRNWELDELIDHFILMPDEQEQIQLKGEIRNLGLLSCLSFFNTK
                     LGSHTKSEIPKTVIEYIAKQLEMNPDVFSEYDWTGRTITYHRSEIRTYFGFRETTLQDV
                     DLLTDWLCKHVLFHSHEFNYVEDKLYQQYRELKIVPPTPDRVERLIRSATYSYEDQFFA
                     SIYQRLSSQTCEGLDKLINLLEKFEDASEAKELLTFHELKSDPGRIGLETIFKELQKLQ
                     TIKELQLPYDLFNDISPKILKKYRKRTATEDIRELRRHPPQIRYTLLSSFFWLRRAEIT
                     DGLIELLIQIIHRINVRAERKVDKELIHNLRKVNGKNNLLFQMAEQALDNPDGIIRDVL
                     YPVISEETLKDLVKEFKHTGPAYKQKVYTIIRSSYSSHYRRMVPRILDMLEFCSNNEAH
                     HPVIEALELIKKYINSNAHFFPESDQVPLDGVIRPSWRYLVVEQETSGEERINRINYEI
                     STLQVLRDKLRCKEIWVPGSERYRNPDEDLPQDFELHREENYKALKKPINPDDFITNLK
                     EEMTKALNKLDQGIPKNNKVRILAKGKGNGRISISPFDPVPEPVNLSRLKAEIMKRWPM
                     TSLLDILKEADLRVNFTDLFKTSASREVISKDLLQKRLILCLYGLGTNAGLKRVSNGEH
                     GEKYKDLLYIRRKYIDKDNLRIAIAEIVNAILRNKMTDIWGEGTTSCASDSKKFGAWDQ
                     NLMTEWHIRYRGRGVMIYWHVAKHSTCIYSQLKSCSSSEVASMIEGVLKHCTSMEVEKN
                     YVDSHGQSEVAFAFCHLLGFQLMPRLKAINSQKLYRPDSGMSDAFPNLQPILTRPINWE
                     LIRQQYDQMIKYATALRLGTAETEAILKRFTRSNLKHPTYQALSELGKVIKTIFLCDYL
                     DSEDIRREINEGLNVVENWNSANGFIFYGKGGEISTNRLEDQEVAVLSLHLLQNCLVYI
                     NTLMIQSVLSEKQWQDMMTPEDLRALSPLIYSHVNPYGTFKLDMMERIPIKERQSEIEN
                     TLHI"
                     /name="Tn3 family transposase"
     gene            complement(278..835)
                     /locus_tag="L6482_RS30120"
                     /old_locus_tag="BCJMU07_p61"
     CDS             complement(278..835)
                     /locus_tag="L6482_RS30120"
                     /old_locus_tag="BCJMU07_p61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010025399.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_061793457.1"
                     /translation="MKFGYMRVSTIDQNLDRQKRQLEKFGCERIFFEKVTGTKRERPEL
                     NRMLEFLRPEDTVVVSDLTRLSRSTKDLIEITELIAQKGAHLKSLKESWLDTTTAHGKM
                     LFTIFAGIAQFERDLTSERTKEGILAAKKRGKYPGRPKADEEKINYALYLIDQGMSRTD
                     AAEKAGISRMTLYRKLQRGEKH"
                     /name="recombinase family protein"
     gene            1176..1631
                     /locus_tag="L6482_RS30125"
                     /old_locus_tag="BCJMU07_p62"
     CDS             1176..1631
                     /locus_tag="L6482_RS30125"
                     /old_locus_tag="BCJMU07_p62"
                     /inference="COORDINATES: protein motif:HMM:NF025502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4304 domain-containing protein"
                     /protein_id="WP_061793456.1"
                     /translation="MASGRDEMIKALKSILIPLLREKGFKGSFPHFRRRLETRIDLISF
                     FFDKYGGGFIVEIGASPTNGFTIKGGTHFPPDKVNTTHHVPCIRLEPTEEHDSFRYDEE
                     PIPPDIYEKLAYEVVSLLPKAEEWWNSDKIARIIKKKAKKQGEINFD"
                     /name="DUF4304 domain-containing protein"
     gene            1841..2518
                     /locus_tag="L6482_RS30130"
                     /old_locus_tag="BCJMU07_p63"
     CDS             1841..2518
                     /locus_tag="L6482_RS30130"
                     /old_locus_tag="BCJMU07_p63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019380480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061793455.1"
                     /translation="MKVEISYFPPEKIITVSGPEATYPFGINGEFQYIVEFSCYIDGNY
                     TDAHEEFFCNWMENQDYYPIYSLAEIYDFQQAEYEELFKLQNIEFNYMKGSRKDTFFVK
                     TIIRSPQQFKEYYPYLYGNGSMLNLSLWSLKQDVFRLEEREYESPYHVKKTKKNRTSLV
                     KLKWIANTPIATLTESSTMFWVGYDGDYITAFSNDEHFSTIDSLQKIIPEKVELVTDDY
                     EWE"
                     /name="hypothetical protein"
     gene            complement(3068..4234)
                     /locus_tag="L6482_RS30135"
                     /old_locus_tag="BCJMU07_p64"
     CDS             complement(3068..4234)
                     /locus_tag="L6482_RS30135"
                     /old_locus_tag="BCJMU07_p64"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_236802736.1"
                     /translation="MENTFTSKINGVYDWLKKQQITFVDQDKKAYKKNNSHSTWDTYCN
                     VMKTYEKWLQKEHGLKDITRAKPKHAEEYMQNLIDKYKRGEGSAYTLSKFPHALHSLQQ
                     SARESKVYRGLKLGNKADLIAMKNGEGIQRKSSESKCLKANASDFDKVQNEIANSRSPQ
                     KEIIMNIHQIQRGVGCRIHEVVKMKGEDITFNSNGSATVYIKGKGGLERWVHVDDKKTI
                     ELLKEQTKDKKDGAYVFPLKNKEGNDKNLSSSMKLAKDVIRSAAERSGVDRDGKTYSTH
                     SARKVYAQERLNAYASMSQEQLQKELTKRIRNYPIDKHGKNRLKEKVDSELQEKRNKIT
                     RDDKDEQKRLRKERDWTHKELCEFMVSIDTGHFRTNIMRYYCDYPKDK"
                     /name="tyrosine-type recombinase/integrase"
     gene            6031..6519
                     /locus_tag="L6482_RS30140"
                     /old_locus_tag="BCJMU07_p65"
     CDS             6031..6519
                     /locus_tag="L6482_RS30140"
                     /old_locus_tag="BCJMU07_p65"
                     /inference="COORDINATES: protein motif:HMM:NF017542.4"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_236802730.1"
                     /translation="MSDKKKKLTTRKATKVIGTQTFINQETGELNEMQVINIEERDANF
                     HKIWLGHIIESLDLIGNKKIKILSFIMQNINSDNLFLMTYDEMAKELSISKPTIVETMK
                     ALQSSNFLKKVRNGQYAINPDVIFKGNKTNRLNVLIQYQKNEEKEQIEQKKDEQHKED"
                     /name="replication/maintenance protein RepL"
     gene            complement(6615..6998)
                     /locus_tag="L6482_RS30145"
                     /old_locus_tag="BCJMU07_p66"
     CDS             complement(6615..6998)
                     /locus_tag="L6482_RS30145"
                     /old_locus_tag="BCJMU07_p66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236802732.1"
                     /translation="MNQKISIEEMTQEMKEKYTLSLEESHKMLLEYGVTKSKNVQVTMR
                     WIRDGKIDGVLIGKGRVETRKWLVSEESLLEFIDFERLTLGDYKAMKKELQELRAFKIG
                     VESKPKTTRKKTTATKEKTEERK"
                     /name="hypothetical protein"
ORIGIN
        1 aaataggtac ggatttccga acgatgataa gtaatggtac gtccggtcca atcatattca
       61 gaaaaaacat cgggattcat ctctagctgc ttagcgatgt actctataac tgtctttggt
      121 atctcactct tggtatggga acctagcttc gtattgaaaa aacttaaaca tgacagcaaa
      181 cccaagtttc gtatttcccc ttttagttga atctgctctt gttcatcagg cataaggata
      241 aagtgatcaa ttagctcatc taattcccaa ttccttttca atgtttttca cccctttgta
      301 gcttacgata tagggtcata cgggaaatac cggccttttc ggcagcatct gtccggctca
      361 tcccttggtc tataagatat agggcatagt tgatcttttc ttcatcagct tttggtcttc
      421 caggatattt tccccgtttt tttgcagcta aaatgccttc ctttgtccgt tcagaagtga
      481 gatctcgctc aaattgggca atgcctgcaa aaatagtaaa caacattttc ccatgtgccg
      541 ttgttgtatc cagccaagac tcctttaaac tttttaaatg cgctcctttt tgggcgatga
      601 gttccgtaat ctcaatcaga tctttcgtgg aacgggataa gcgagttaaa tccgatacaa
      661 caacggtgtc ttctggacga agaaactcca gcatacggtt tagttccgga cgttcccgtt
      721 tcgttcccgt tactttctca aaaaagatgc gttcacatcc aaatttttca agctgtcttt
      781 tttgacgatc caaattttga tcaatagtcg aaacacgcat gtaaccaaat ttcattcata
      841 tcctcacctt tcagcataat tgtaccaaaa ctatgaaata attaatattg ttacaaatta
      901 ttttgttaca tatttttgtt acgttgttta tgctagattg gcagacaaaa tatatttttc
      961 acaccatgta acagaaatgg tcgtttttgt tacagcgatt acataacaaa tactacttta
     1021 taaaaaagta gtaaagttaa agttaaaagc ttcagttact ctattccgca atcgggccag
     1081 attctggagc aaggttaatt gaactaacgg tgcaggttaa cgtaaggagc actcggataa
     1141 aataggattt aataaattaa aaggagtagg gtataatggc ttctggacgt gatgaaatga
     1201 ttaaagcact gaaatcaatt cttattccat tattaaggga aaagggattt aaaggttctt
     1261 ttccgcattt tcgacgtaga ttagaaaccc gaattgacct tatctctttt ttctttgaca
     1321 agtatggtgg tgggttcatt gttgaaatag gtgcttcacc aaccaatggt tttacaatca
     1381 agggtggaac tcactttcct ccagataagg taaacacaac tcaccatgtt ccatgtattc
     1441 gacttgaacc aacggaggaa cacgactcat ttagatatga cgaggaacct attccacccg
     1501 acatttacga aaaattagca tatgaggttg tttctctttt acctaaagcc gaggagtggt
     1561 ggaattcaga taaaatagcg cggattatta agaaaaaagc aaaaaaacaa ggtgaaatca
     1621 attttgatta aagatatttc ccttattgtg taacgggagc gttagtttaa caagctaagg
     1681 tagcaatcgg gcgcactcct tcaacaaggg atgcttttct tattgaacaa acggaggtta
     1741 gatcaataag ctgataatac taataagcca gcgaaaacaa tgatacacgt agtaatataa
     1801 taaaaatata attattcgaa ccttttaaga ggcgattaaa atgaaggtag aaataagcta
     1861 cttccctccc gaaaaaataa taactgtatc ggggccagaa gccacctatc cttttgggat
     1921 taacggtgag ttccaatata tcgtagagtt ttcttgttat attgatggta attatacaga
     1981 tgcccatgaa gaattctttt gcaattggat ggagaaccaa gattattacc ctatttattc
     2041 ccttgcggaa atctatgatt ttcaacaagc agagtacgaa gaactgttta agctgcaaaa
     2101 tatagagttt aattatatga aggggagtcg taaagacacg ttctttgtaa aaacaattat
     2161 acgaagcccg caacaattta aagaatatta tccttatttg tacgggaatg gaagcatgtt
     2221 gaacttatca ttgtggtcct taaaacagga tgtattcaga ctagaggaaa gggaatatga
     2281 atcgccttat catgtaaaaa agactaaaaa aaaccgaacc agtctcgtta aattaaagtg
     2341 gatagccaat acgccgatag caactttaac tgaaagttcg acgatgtttt gggtagggta
     2401 tgatggcgat tatattactg cgttctcaaa tgatgaacac ttttcaacaa tagactctct
     2461 tcaaaaaata atacctgaaa aggtcgagct agtgactgat gattatgagt gggagtaatg
     2521 ttctcccgtt ctataccctt ttgaactaaa ccagctaata gaagcccttc tgttcttcaa
     2581 caatcgggcg cgattgttga agaacactta gatttataaa gactttattg ctaaagtatc
     2641 cttatttaac aactttattt tacccccatc caaaacaaga cgggggtatg tgtgttgaaa
     2701 gtatttaact taaacaactt attataacct tgacatttcc ttattgcggg tggtgacagc
     2761 ttcgctggta ctacccctat ttgagaaaaa aataaaaaac cacattttat gcggttttgc
     2821 gaggattctc cctatttttt tcccttcttc cctttgtccg catgtttggg ttggcttccc
     2881 acgtaggcta acatggggat tctcattgta aagtcaccgc ttcgctctcc gctacactac
     2941 ggactttaca acgagcctcc accccatgtt tctttgccaa cgtgccaccc cataccatgc
     3001 tccgctaagc ttccctaaat agcgatttaa ctcgattagc ctcgctaaaa caaaccaaaa
     3061 aacaacatca tttatccttc ggataatcac agtaatatct cataatattc gttctaaaat
     3121 gccctgtatc aatgcttacc atgaattcac atagttcttt atgagtccaa tcacgttctt
     3181 ttcttaatct tttttgttcg tccttatcat ctctagtgat tttattgcgt ttttcttgca
     3241 attcactatc tactttttct tttaatctat ttttgccatg tttatcaatt ggatagttac
     3301 gaattcgctt tgttaattcc ttttgtaatt gctcttgaga catactcgca taagcgttca
     3361 gacgctcttg tgcatacact tttcttgctg aatgtgtgct atatgttttt ccatctctat
     3421 ctacgcctga acgctccgct gcggagcgaa tcacgtcttt tgctaacttc atagatgatg
     3481 acaaattttt atcattgcct tctttgtttt tcagtggaaa tacatatgca ccgtcttttt
     3541 tatcttttgt ttgctctttt aatagctcta tcgtcttttt atcgtctaca tgaacccaac
     3601 gttccaaacc gccttttccc ttgatataca ccgttgctga accgttagaa ttgaatgtga
     3661 tatcttcgcc tttcattttg acaacttcat gaatacgaca acctacacca cgttgtattt
     3721 gatgaatatt catgatgatt tctttttgtg gagaacgaga atttgcaatc tcattttgca
     3781 ctttatcaaa atcacttgca tttgctttca aacacttgga ttcactgctt tttctttgaa
     3841 ttccttcacc atttttcatt gcaatcaaat ccgccttgtt tcctagcttt agaccacgat
     3901 aaacctttga ttctcttgct gattgttgca aagaatgtaa agcatgagga aatttggaaa
     3961 gcgtgtatgc cgaaccctcg ccacgcttgt atttatcaat caaattctgc atatattctt
     4021 ccgcatgttt gggcttcgct cttgtgatat cttttagccc atgctccttt tgaagccact
     4081 tctcataggt tttcatcacg ttacaatacg tatcccaagt agaatgagag ttatttttct
     4141 tgtacgcttt tttatcttgg tcaacaaaag tgatttgttg tttctttaac caatcgtaaa
     4201 caccgtttat tttgcttgta aaagtgtttt ccatacaaag acacccccca aagagtaaaa
     4261 attgattcta gggcatgtta cggctcttaa aaggcataat ccacacccac caaccgcctt
     4321 gcggttgctg ttatataaaa atggttttta aatgtggttt taaaaataaa aacctaaatt
     4381 caaaagcaaa tcgtattttt ttgccataaa accatgcaaa aaaacactct tccctaacca
     4441 ccgagggtgg ttctatccta tgtttttctt cgaaaaaaat acgatagaac cccctcgaac
     4501 ataaccccat atcccatatt cagttgttct cccttcgaaa gttttactcg atgccctcgc
     4561 ttgtcgttcg ctttgccttc ggcaaacctc acgccaacct cgtgcaacgc tccatcttgt
     4621 tttatcgctt ttcaccttcg gtgaaaacct caaaacaagt tgtcgcttct cgacactttc
     4681 tcatgttcag tcagccacag agtgacagtt tgcatagcgg tctgataacg ctcagatata
     4741 cgtttattgc attttgttac atttgatttt tattacataa aacatttttt tgatacgttt
     4801 tgaaacacta tccatcatct ctttcaatca acattcatct actattctcc ttatatctcc
     4861 tccttttttt aattgtattt gatgtgcgtt tcttctcttg attcgaccct aaaacgaaat
     4921 tgtctaaagt tatgattgtg agtgttggaa tatttggttg caacagagac tgttgcctta
     4981 cttgtactac atggtattgc ttgtaggttg atatattcat attcaccccc aatcgttttc
     5041 agaagttgtc tttttgacat cttccttaca gtacgtttta cgcatataaa acgtttaggg
     5101 attctctttt gattgtctgc tttcttgttt ttttactctc tctcagcgaa ccgaaaggtt
     5161 tcatcaaaca attccaaatt ccattggcgg tttttaagtt ccttctaatc ataaccagtt
     5221 ttttccattt ttcaattaaa ataatttaat ttaatttcgt ttaaaatcct tgagtttcta
     5281 agacaatcga ggaaacgttc cgattggtgg aagcaaccca atcttacgat tggacaaaca
     5341 tggacaagac tgttcctcct taggcgaaag gacaagtaat catatccgat tcggaaacgg
     5401 ttttccctta ctttttgggt tgaggttttg ggcggggtgt tggggctggc cccacatttc
     5461 actataaaaa aataaaccgc atatgtacgc tctgaacgtt cgtcacgtat acttgcatgt
     5521 ccaaatcgat aacctattaa tctccttttt ggtgtatcgg tttggtgatg caaacccacg
     5581 tgacgacaac attctgtcaa atgcttgaag gtggagattt tttggaacgt agtggagaaa
     5641 aatactacct gtcatttgat agagtgttga gtggttcatg aaaaagtaaa cgtaatagac
     5701 atgtagagtg agaacttttt aacatcgaaa acctaaacaa taatacatag ataagagtga
     5761 attacttttt aaaattgata taaagccttg atatatatgg gtttgttgga cttttttttg
     5821 gttaaatttt ttttatcttt tggttaaatt ttttttaact cttagtaaag tttcatttta
     5881 ctttttggta aataattttt aaccaatatt tttttactga tttttttgtg gtaaaataaa
     5941 tttgtccaaa aagtaaagtt ttatttaaac tttttaaagg atatgaaaaa aaatctttga
     6001 agtaaataaa caagcaaagg aggtaaattc atgagtgata aaaagaaaaa attaactact
     6061 agaaaagcta caaaagttat tggaacacaa acatttataa accaagaaac aggcgaatta
     6121 aatgaaatgc aagtcattaa tattgaggaa cgagatgcaa atttccacaa aatttggtta
     6181 ggacatatca ttgaatcctt agacctaatc ggaaataaaa aaatcaaaat actatctttc
     6241 attatgcaaa atatcaattc tgacaatcta tttttaatga cttatgatga aatggcgaaa
     6301 gaattaagta tctcaaagcc taccatcgtt gaaacaatga aagctttaca atcttcgaat
     6361 ttcttgaaaa aagttcgaaa tggacaatat gcaataaacc ctgatgttat ctttaaaggc
     6421 aataaaacaa accgtttaaa tgtattaatc caataccaaa agaacgaaga aaaagagcaa
     6481 attgaacaaa aaaaagacga gcaacataaa gaggactagt ctttacatat actcgcctaa
     6541 gctattttcg ttattaatat tatactataa aaagattgaa aaaacctgta agaggtggga
     6601 aattcccacc ttttttattt tctctcttct gtcttttctt ttgttgctgt tgtcttttta
     6661 cgtgttgttt ttggtttgct ttctacccca attttaaacg ctcttagttc ctgtaattcc
     6721 tttttcattg ctttataatc ccctaatgtt aagcgttcaa aatcaatgaa ttctaacagg
     6781 ctttcttcac ttaccaacca tttacgagtc tctacacgac cttttccaat taaaacacca
     6841 tcaattttac catcacgaat ccaacgcatt gttacttgca cattcttgct ttttgttact
     6901 ccatattcca acaacatttt atgcgattct tctagtgata acgtgtattt ttccttcatt
     6961 tcttgcgtca tttcttcgat tgagattttc tgattcatcc taattccccc taatatactt
     7021 taaagtatat gtatttttat tacatttaat ggttgatata tttattttaa attaaatcta
     7081 cttaaaagta aatgtatttt aaaataaatt tatttataaa tatttttact atgatttata
     7141 tctactataa attacattca attaaaagta aatatatcct ttagtaaatc taatattttt
     7201 attaaattta ctttcgagta aatctataat tagctatgac attactccgt tatacatgta
     7261 ctcgcaaact ctaaaacctt gaaaacaaag gagattaggt gagtcttagc gaatcctttt
     7321 acttaaaaat acatgtatta ttatcaatat aattactttt gagtaaatgt atttttatat
     7381 actagtttta cttttaagta aatttattaa atttactcta taatacattt attattaaaa
     7441 taaatttact ttataataca tttacttaaa aatctatcta tttggggtag taccagcgaa
     7501 gctgtcacta cccgcaataa ggaataaagt taaatatgca aagtattttc tatttctgat
     7561 tgcctttctt tgattggaat tctctccatc atatccaatt taaaggtacc ataaggattt
     7621 acatggctat agataagtgg acttaacgcc ctcaagtctt caggtgtcat catatcctgc
     7681 cactgctttt ccgataatac agattgaatc atgagtgtat taatatacac gaggcaattt
     7741 tgtaataaat gcagagaaag aacggcaacc tcttggtctt ccaatctatt tgtagaaatt
     7801 tctccacctt ttccataaaa aataaagcca ttggcggaat tccaattctc tacaacatta
     7861 aggccttcat taatttctct ccgaatatct tcagaatcca aataatcaca caagaaaatg
     7921 gttttaataa cttttcccag ctcagacagg gcttgataag tcgggtgttt taagttactt
     7981 cttgtaaatc gttttaagat agcctctgtt tcggctgttc caagacgcag ggctgtagca
     8041 tatttaatca tttgatcata ttgctgacga atcaattccc agttgatagg tcttgtaaga
     8101 atcggttgta aattagggaa agcatcactc atgccactat ctggcctata aagtttttgt
     8161 gaattgatag cttttaatct tggcataagc tgaaaaccca agagatggca aaaggcaaaa
     8221 gctacttcgc tttgaccgtg actgtctaca taattttttt caacctccat gcttgtacaa
     8281 tgctttaaaa ctccctcaat catagacgct acctcagaag aagagcatga ttttaattga
     8341 gaatagatac aggttgaatg tttagccaca tgccagtaaa tcattacacc tctgccccta
     8401 taccgaatat gccattcagt cattagattt tggtcccacg caccaaattt cttggaatcg
     8461 gaagcacagg aagttgttcc ttctccccat atatcggtca ttttatttcg caaaatggca
     8521 ttaacaatct cagcaatagc gatacgtaga ttgtctttat caatgtattt tcgccgtata
     8581 tataataaat ctttatactt ttcgccatgt tccccatttg aaactcgctt tagccctgca
     8641 tttgtcccca aaccgtataa acataagata agcctcttct gtagcaaatc tttactaatg
     8701 acctctcttg atgcagatgt tttaaaaagg tccgtaaagt ttacacgcaa atcagcttcc
     8761 ttgaggatat ctagtaaact cgtcattggc catcgtttca taatttcagc ttttaggcgt
     8821 gataggttaa ctggctccgg caccggatca aaaggagaaa tagatattcg gccatttccc
     8881 tttccttttg ctaaaatgcg aaccttatta tttttaggga ttccttgatc taatttattt
     8941 aacgcctttg tcatttcttc ctttaaattt gtgataaaat catcgggatt aataggtttt
     9001 ttcaatgctt tatagttctc ttcacggtgc agttcaaagt cctgtggcag atcttcatct
     9061 ggattacgat atcgttccga accaggtacc catatttctt tgcatcgcag cttatcacgt
     9121 aagacttgca aggtgctaat ctcataatta atacgattga ttctctcttc gcctgaagtc
     9181 tcctgttcca cgactaggta tctccagctg ggacggatca caccatctaa tggaacctgg
     9241 tcagactctg ggaaaaagtg agcatttgaa tttatatatt tttttataag ttctaacgct
     9301 tcgattactg gatgatgagc ctcattattt gaacaaaact ctaacatatc caaaatacgg
     9361 ggaaccatcc tgcggtaatg ggaactgtac gatgaacgaa taatcgtata taccttctgt
     9421 ttgtaggcag gccctgtatg tttaaattcc tttactaaat ctttaagtgt ttcctcgctg
     9481 ataacaggat aaaggacatc ccgaataatt ccatctggat tatctaacgc ttgttcagcc
     9541 atttggaaca atagattgtt tttcccattt accttcctta aattatgtat taattcttta
     9601 tccactttac gttcggccct aacattaata cgatgaataa tttgtataag cagttcaatc
     9661 agaccgtctg taatttctgc tcttcgaagc caaaagaagg aagataaaag tgtgtagcga
     9721 atctggggtg gatgccggcg caattcacgg atatcctctg ttgctgtacg ctttctatat
     9781 ttctttaaaa tttttggtga aatatcgtta aacaaatcat agggtaattg cagctctttt
     9841 atggtttgaa gtttctgaag ttctttgaag attgtttcta atccaatgcg tccaggatcg
     9901 gactttaatt catgaaatgt taataattct tttgcttctg aggcgtcttc aaatttttct
     9961 aaaagattaa ttaatttatc caacccttca catgtctggc tagaaagtct ttgataaata
    10021 gaggcgaaaa attgatcttc ataggagtag gtagcagagc gaataagacg ctctacacga
    10081 tctggggtgg gaggcacaat ttttaattct ctgtattgtt ggtataactt atcctcaaca
    10141 tagttaaatt catggctatg aaataacacg tgcttacata accagtctgt caataagtct
    10201 acatcctgca gagtagtttc gcgaaatcca
//
