LOCUS       NZ_AP022941            13310 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain J7 plasmid pJ7_5, complete sequence.
ACCESSION   NZ_AP022941
VERSION     NZ_AP022941.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203332
            Sequence Read Archive: DRR206372, DRR206396
            Assembly: GCF_021655135.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13310)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 344x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655135.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 07:11:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,087
            CDSs (total)                      :: 5,938
            Genes (coding)                    :: 5,644
            CDSs (with protein)               :: 5,644
            Genes (RNA)                       :: 149
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 294
            CDSs (without protein)            :: 294
            Pseudo Genes (ambiguous residues) :: 0 of 294
            Pseudo Genes (frameshifted)       :: 177 of 294
            Pseudo Genes (incomplete)         :: 134 of 294
            Pseudo Genes (internal stop)      :: 43 of 294
            Pseudo Genes (multiple problems)  :: 50 of 294
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022941.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13310
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="J7"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pJ7_5"
                     /geo_loc_name="Japan:Tochigi"
                     /collection_date="2006"
     gene            join(12635..13310,1..968)
                     /locus_tag="L6482_RS30040"
     CDS             join(12635..13310,1..968)
                     /locus_tag="L6482_RS30040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_220784856.1"
                     /translation="MDFISFKEIESQVDSDVIENIKSQCYERVFSRSELTIVGKTKTGT
                     RYRSRNIYETVFNEDKYKRIVRQYFIDHIHQKEEKPSKKEQNKTTKKTPTKRKKKEIGK
                     NITQREFFEHIFRDLFDNKRGEEDKKGRRIYLKSFLFDIDKESKLINEKKVVSNVEKAI
                     NYGKKCGYFTPALFISHKFFTKEMLSLMGVILLDFDLDKANVVMTKEQVYKYIKKKLKV
                     EPSMVWDTSTKGNYQTAILIEPMVGTPKSIHLYEQIVKEMIRKLGDVCDKSAFESNHIF
                     AIGKNNIRTGKYTRFYSDTVHSINEFRWLLDERDKRKKKEQKVVNFKEESILRHPAIDS
                     LLTGKNIRFRDHGCFTAALVLRFLGKDELEAETYLLSNWLGNVNNESYDHKFTEREVLK
                     CVKHAYSGRYRSFLSSWVETCTGIECNLRGYFRTTYVKTGAYNTDSKERILEYLRKNDG
                     IVESNAPLLAEELGLSKKTLERLLPQMRKDNKIHYETVIGKGKKTTYTLVEQIELIPTI
                     DIEEFKPTIQRVEEIGYLEQIMNDFVSLRV"
                     /name="hypothetical protein"
     gene            complement(1652..1924)
                     /locus_tag="L6482_RS30045"
                     /old_locus_tag="BCJMU07_p501"
     CDS             complement(1652..1924)
                     /locus_tag="L6482_RS30045"
                     /old_locus_tag="BCJMU07_p501"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_220784858.1"
                     /translation="MKRIDFHEIDFEKMKIVENYNYHGEEYHSYHLVFEIPKLPNTKLL
                     TVVDVWENEWNVKRIHQLEPKITYELDDALKEKFVAALKKVLAIS"
                     /name="hypothetical protein"
     gene            3400..4740
                     /locus_tag="L6482_RS30050"
                     /old_locus_tag="BCJMU07_p502"
     CDS             3400..4740
                     /locus_tag="L6482_RS30050"
                     /old_locus_tag="BCJMU07_p502"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236802724.1"
                     /translation="MDSKKLAQTIGKMEDALDRIHRRKSKEKEEAIEKNEEIEKNGGTE
                     SDFVPVPKAWSDRTVETYKDMMKSFIRDVNKETGFADMKKIAGQFENYMQKRLDNYHSG
                     ERSESYNVKTLIAAVKAYNEGVLSTAAFTKVPENRANFVVKNVDGMQKHLQETNVIRHS
                     GSSKVLTATGKEVESVLDNIKNNGRGMKDDRYDIRKISYVTSKLQYAAGGRITNTLKVT
                     VAQVKEVLATGKMTFVKDKGGLTREVVDIKLDTELRAELTKMIDNKKDDTRAFIAKKMD
                     GKFLSINETRKKISRFVKEAGKHLTREETVSVKDRDGKKTSITVTKEFSTHSFRKAHAL
                     EKVVEYLDKFRTREEAEAYIKERSKAEPKIWSKYKREINRLNRDRNKNREMTRLEMAYY
                     ACSTSLGHFRLGIVETYYAPPEMVLEHYLNTFGEEYEGFADETKRGR"
                     /name="hypothetical protein"
     gene            4940..5467
                     /locus_tag="L6482_RS30055"
                     /old_locus_tag="BCJMU07_p503"
     CDS             4940..5467
                     /locus_tag="L6482_RS30055"
                     /old_locus_tag="BCJMU07_p503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289453.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_236802726.1"
                     /translation="MESNQEAKEYKMKEVSEITGLSNDLLRTYEEEFNLQIARTSGGHR
                     RYTEEDINTFVTIKKKIQEQNWSYKKIRSWLSGEEMSVPIQEHQVVSNLEKKLDKQAEM
                     IQDLTDKLDQSLTLQAAMMQKMMELQTEKKQLQTIVENRNQDFINTLIEEKRKDREERE
                     NKKPLFKRLFGR"
                     /name="MerR family transcriptional regulator"
     gene            6063..6476
                     /locus_tag="L6482_RS30060"
                     /old_locus_tag="BCJMU07_p504"
     CDS             6063..6476
                     /locus_tag="L6482_RS30060"
                     /old_locus_tag="BCJMU07_p504"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236802728.1"
                     /translation="MTIYTKEAIKILGLSGSTIGHYVRVLENHKYKFERMNGRVTFSEN
                     DLEVMKIMKKVIREKTISQEKAAKIVLEKLLEEKEEEKEMTMKEMAAEIRKISQTLDEL
                     KKSVVDMEEIKEILNQLYVNQQKKGFIKKIFGK"
                     /name="hypothetical protein"
     gene            6706..7539
                     /locus_tag="L6482_RS30065"
                     /old_locus_tag="BCJMU07_p505"
     CDS             6706..7539
                     /locus_tag="L6482_RS30065"
                     /old_locus_tag="BCJMU07_p505"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236802716.1"
                     /translation="MTKQTDTLQIRGGSEDVLQIKEMIEKTGMTGKEFLPVMLQAIKKE
                     LAVAGAEELNFTADIEEINNHMLRVYKLLGSITERSKSVLDTKTERLKEEVAEKNEQLQ
                     QERAASEKQKEEFEKQLKEQQEEIAKLKKELKEEKDFSSTIDKENREQKKQLKVYTEVI
                     ETKKDENERLERRMEANKKAAEENEQLKKEKEELQKKTIEQEKEIEKLQENSKRDLEML
                     ELKLKNEHMQEINGLNQRHIEQIQSEQKTPKKASTTPKTSGTKPKQAAETETKTK"
                     /name="hypothetical protein"
     gene            7660..8115
                     /locus_tag="L6482_RS30070"
                     /old_locus_tag="BCJMU07_p506"
     CDS             7660..8115
                     /locus_tag="L6482_RS30070"
                     /old_locus_tag="BCJMU07_p506"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236802718.1"
                     /translation="MYVKTYQPEDIFAFWRKIQSGTPVEYDFKYVSLEKRGMLQFIIYV
                     HRTKFYKTRGNNVEQFYESFIQKKKNRFDSFFVLLNEQGKIQSKLYEHEVIDEIYEGIE
                     CMYSTLPVERDPNELMKKIQHLNEHEEYDSWMKKLKIQRFKSLFKEK"
                     /name="hypothetical protein"
     gene            8280..8843
                     /locus_tag="L6482_RS30075"
                     /old_locus_tag="BCJMU07_p507"
     CDS             8280..8843
                     /locus_tag="L6482_RS30075"
                     /old_locus_tag="BCJMU07_p507"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_220784842.1"
                     /translation="MQKIGYARVSSKDQLLDSQLMMLEEYGCDEIITEKVTGVAKDRKL
                     FELVERMEPGDKLIIVSINRLGRSTLQSIALAEEMRNKGLELVILEHQIDTTTTNGKLF
                     FEIMCSFADWERSNLKEKQERGIQAARLKGKHLGKPRQWTKSGMEEAVKMYEEERIVSE
                     IVEITKVSRAALYRELKKRGITRT"
                     /name="recombinase family protein"
     gene            8843..9016
                     /locus_tag="L6482_RS30080"
                     /old_locus_tag="BCJMU07_p508"
     CDS             8843..9016
                     /locus_tag="L6482_RS30080"
                     /old_locus_tag="BCJMU07_p508"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_220784844.1"
                     /translation="MKKENVKAAMQGVKHSMQTDGFEITEEQTKLVAKQLRNEITEEEF
                     LQEVRKRISKGQ"
                     /name="hypothetical protein"
     gene            9018..9191
                     /locus_tag="L6482_RS30085"
                     /old_locus_tag="BCJMU07_p509"
     CDS             9018..9191
                     /locus_tag="L6482_RS30085"
                     /old_locus_tag="BCJMU07_p509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_220784846.1"
                     /translation="MGELIRFPKRIVELTEEEHKRFIEYKENMIAAETKAEMQYYYNKV
                     KYIIEQANKRKK"
                     /name="hypothetical protein"
     gene            9207..9641
                     /locus_tag="L6482_RS30090"
                     /old_locus_tag="BCJMU07_p510"
     CDS             9207..9641
                     /locus_tag="L6482_RS30090"
                     /old_locus_tag="BCJMU07_p510"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_220784848.1"
                     /translation="MGNVYTQTWEEILENRPNMRADFEQTKPLLEIEDVEIWDYEEKEV
                     VKDWYSGGYVKRDVKAIFVKFKGEERRIRFQTYFQPTVGEWVTSDMAPVHVEGKCHVCK
                     HFKTSIEDDSSSCTCINVLDLKYLLDKIKQMKEHRLKFIL"
                     /name="hypothetical protein"
     gene            9657..10367
                     /locus_tag="L6482_RS30095"
                     /old_locus_tag="BCJMU07_p511"
     CDS             9657..10367
                     /locus_tag="L6482_RS30095"
                     /old_locus_tag="BCJMU07_p511"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236802720.1"
                     /translation="MGELYTIYFKDGLKNELQVKFYYKTYERDYGDSDFAYDYDHDDDN
                     FTHIDWIEVKKNHIFKINYNFTGFINSTYYANEEVQKNANKYKDEVLDFCKQITPGQKV
                     EDGVIQDDMDNPVVTTEKIDAMFQKCKEIRFSEGFGEPHFIVGDPEMEYGMEYGSCVLL
                     LTVKNVWQIKGEKEIELEHFEIDFQTAGKEIMGYSYLPFDSRTYKHFEEKFEKALLPEI
                     KKQLKVWQLQQLFK"
                     /name="hypothetical protein"
     gene            complement(10647..11024)
                     /gene="qacH"
                     /locus_tag="L6482_RS30100"
                     /old_locus_tag="BCJMU07_p512"
     ARG             complement(10651..11024)
                     /name="qacH"
                     /gene="qacH"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacH"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(11024..11587)
                     /locus_tag="L6482_RS30105"
                     /old_locus_tag="BCJMU07_p513"
     CDS             complement(11024..11587)
                     /locus_tag="L6482_RS30105"
                     /old_locus_tag="BCJMU07_p513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002078386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_077947201.1"
                     /translation="MEVIMSISKHANKKKNILIAASEIVKEEGVVKLTLEAVAQRAGVS
                     KGGLLYHFPSKEALIKGMVEDWINNYFECINTLVNNDDDNAIGKWNRAYLKSTFSDLEN
                     NNLNSALMAAMFINPDLLDEFRQRYDILHTKLITDGIDPVKITITRLSIDGLWFSEIFG
                     MAPLNEELKTQVFDELINMIQEDE"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            11771..12238
                     /locus_tag="L6482_RS30110"
                     /old_locus_tag="BCJMU07_p514"
     CDS             11771..12238
                     /locus_tag="L6482_RS30110"
                     /old_locus_tag="BCJMU07_p514"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236802722.1"
                     /translation="MGENYITVVNPHFQDSTPYSPNKFFKVFIKNHQEEMEGYEFSFSW
                     AYTSATEIRIKIYACLKDIPESEYCFKEFIIDVPKTIEAEIWSRIELIDNQIYEGFIYE
                     EYGNKIMSALSKGYDAVLIKLVRNFKAGILDQSQFESSVRLLKSFRLQQLF"
                     /name="hypothetical protein"
     ARG             complement(10648..11024)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="60.75"
                     /coverage="116.82"
ORIGIN
        1 tggtttggga cacaagcacc aaaggaaatt accaaacagc gattttaata gaaccgatgg
       61 tgggaacgcc gaaatcaata catttatacg agcagattgt aaaagaaatg attcggaaat
      121 tgggtgacgt ttgtgataaa tcagcctttg aatcaaatca tatttttgcg atcggaaaaa
      181 ataatattag gacagggaaa tatactcgtt tttacagtga tacagtacat agtattaatg
      241 aatttcgttg gttattggac gaacgagaca aacgaaaaaa gaaagaacaa aaggtagtaa
      301 atttcaaaga agaaagcatt ttgagacatc cggcaattga ttcattatta acaggtaaaa
      361 atatcagatt ccgagaccac ggatgcttta cggcggcttt agttcttcgt tttttaggca
      421 aagatgaatt agaagcggaa acgtacttgt taagcaattg gctagggaat gtgaataatg
      481 aaagctatga tcataagttt acagaacgag aagtgctgaa atgtgtaaag catgcttaca
      541 gcggtcgtta taggtcgttt ctctcttcat gggtagagac ttgtacggga atcgaatgca
      601 atctgagagg atatttcaga acaacatatg taaaaacggg tgcatataat acggattcta
      661 aggaaagaat cttggagtat ttaagaaaga atgatggaat tgtagaatca aatgctcctt
      721 tgcttgctga agaattagga ttaagtaaga agacattaga aagattgctt ccacaaatga
      781 gaaaagataa taaaattcat tatgaaactg tcattggaaa aggaaagaaa actacatata
      841 cgctagttga acaaatagaa ttgattccaa caattgatat agaggaattc aaaccaacta
      901 ttcaaagagt agaagaaata gggtatttgg aacagataat gaatgatttt gtaagtttac
      961 gagtatgatt ttgtttttca taagatgcag acccccatgt tttgggggtt ttttattttt
     1021 catgacatgc gcaaaccatt ggtatgactg ggttttaaaa accgccaaat tacataccat
     1081 tatcaattaa tatcactact atttaatttc cgtaacatgc agacccccat gttttttaaa
     1141 aaaagtgaaa agagtaaacc tcgtgaaccc ttgatatgac tggattttaa ataccctcaa
     1201 attacatacc attatcaacc ttgttttgat ctttctttcc tccacactta ttctctttcc
     1261 ctctctcttt tctccacttc tatacagtat ctttatttac tggagggagc ttgcgaacga
     1321 cacctggggg tggtgggggc ggtctatccc ccgcggtctt ctctttttct ttcttctcct
     1381 gttattcact attggagtct cttttactgg cttgggggat tttttctgtt tttatcttct
     1441 atttctctga acaaataaaa accaacgatc tatacagacc attagttttt attttcacaa
     1501 gaaagtaaag taacaaaatg taatttagat ttattgattc acatagaaac aacatggaac
     1561 aaacaaaaga aaataacatt aaaaaatacg aagaaacaca atgcttttta cattgtagtg
     1621 tttttcaaca cacttttttt ataatcaaaa atcatgatat agcaagcact ttttttagtg
     1681 cagcaacaaa cttttctttt aacgcatcgt ccaattcata tgtgattttg ggttctaatt
     1741 gatggattcg ttttacattc cattcatttt cccaaacatc aactacagtt aataattttg
     1801 tattgggtaa tttggggatt tcaaaaacca aatggtatga atgatactct tcaccatgat
     1861 aattatagtt ttccactatt ttcatttttt caaagtcaat ttcatgaaaa tctatccttt
     1921 tcatttttat tccctcgtcc ctttggaatt tgaccgcata ttttccgttt taacacacct
     1981 gtttttcatt ttgaatagtc caagacctga acaaaccaaa acgtcaggaa agtgcctttt
     2041 aattgatttt tttggaacaa aagttgttgc attgtgctag aatttaagta tatttagctt
     2101 aactttaaaa aacccgtcat acacgtatga caacgcatga ttttggaaga aaatcttaat
     2161 tatgaaaata aatgaatata ggggtgtgtt gcaagttcaa cataaattat ttaccttgtc
     2221 acactcataa aaaaggacgt tgataactag aatatagata tccaacgttc ttatttttat
     2281 gtagagaggc aaacaataag aagaatcttt ttacccgaag atgttatgta tctgtgaaac
     2341 ttgcttgcaa ggatcacaat taaaaacaaa taaaaatgag aaagggaaaa atttagaatt
     2401 tacgttggtt agtttgaaaa gaaataaaga agttttgatg caatgtaact acagtttttt
     2461 ggaagaatta cagtttcata tttttaaaat ttagcagtac caaacaaaaa aataaaattt
     2521 gaggaggaaa aaagatgact ggatttgatt gatcaccaaa ttaaaatgtc cgcttttaga
     2581 ttgatcaact agaagaaatt gaacacgttg aaatattgtt ttatgtttta gatttatcgc
     2641 ttcaaagtcg caaggtatgt acggatcaat gtattcgtgg aaggacctca atcttccaaa
     2701 tcgcaagacc gatgtctctt agttcgaaga aactaagaac atacgcaaca tccgcaagga
     2761 tgggagtgca gttactctct taactgtgta atacaaaatt atattgtgac gattcgtatt
     2821 ggaattatgg ttgtaggcga gttttttcag ttggcggatt gtgtatttgt atttgtgttc
     2881 gaaccacgtt tcgaacacaa atacaaatgc gacaagccaa agacaactga aaaacacgag
     2941 ttgctttagg cgaaattttt ctttgcgcct actggcgcaa agaaaaatgg agatgctcta
     3001 ggcggaatat ttctattgtt gcgctggcaa gcgtaacaat agaaatgtgg agttgtattt
     3061 gactttgatc tcccacggtt acgtcgctta tctatgcggc ttgtttttta gccgcaatag
     3121 atgtgcgact taaccagggg gagacgtact tttctaccct ggtgaggatg ttattcatca
     3181 tttgtgaagc gttcttcttc acaaatgata gtaataacac cggcttttac ttactttttg
     3241 aatacgttct tcattcaaaa agtttagtaa aaaaggtttt ggcattttgt atttgccttt
     3301 gaccttgaat tttatctttt gcatttattt tttgctttgg aagttgatgt tttcggacgt
     3361 tttgatataa accattcaat gaataagggg gtgttgggaa tggatagcaa gaaattagcg
     3421 caaacaattg gaaaaatgga agatgctttg gatcgtattc atcgaagaaa atcgaaagaa
     3481 aaggaagaag caattgagaa gaatgaagaa attgagaaaa atgggggaac ggaaagcgat
     3541 tttgtaccag ttccaaaggc atggtcggat agaacggttg aaacatacaa agatatgatg
     3601 aaatcattta taagagatgt gaataaagaa acgggctttg ctgatatgaa gaaaattgcg
     3661 ggacaatttg agaattacat gcaaaaacgt ttggataatt accattctgg ggaaagaagt
     3721 gagtcttata atgtaaaaac actcatagca gccgtgaagg cttataatga aggcgtgttg
     3781 agtacggcgg catttaccaa agttccggag aatcgtgcaa atttcgttgt aaaaaatgta
     3841 gatggaatgc agaagcattt acaagaaaca aatgtgattc gtcatagtgg ctcaagtaaa
     3901 gtgctgacgg caacagggaa agaagtcgaa agtgttttgg acaatataaa aaataatggg
     3961 cgtggaatga aagatgatcg ttatgatata agaaaaattt cttatgtgac atcaaagcta
     4021 caatacgccg caggcggtcg aattacgaat acattgaaag ttacagttgc acaagtaaaa
     4081 gaggtattag cgactgggaa aatgacattt gtgaaagata agggcggttt aacaagggaa
     4141 gttgtagata taaagttaga tacagaatta cgtgcggaat taacgaaaat gattgataac
     4201 aaaaaggatg atactcgtgc attcatagca aaaaaaatgg atggtaaatt tttaagcata
     4261 aatgaaacta gaaaaaagat ttctaggttt gtaaaagaag caggaaagca tttgacacgt
     4321 gaagagactg taagcgtgaa ggatcgggac ggaaagaaaa ctagcattac agtaacaaaa
     4381 gaatttagta cacatagctt taggaaagca catgctttgg aaaaggtcgt ggagtatctt
     4441 gataagttca gaacaagaga agaagccgaa gcatatataa aagaacgatc aaaagcagaa
     4501 ccgaaaattt ggagcaaata caagcgagaa atcaatcgtt tgaatcgtga tcgaaataaa
     4561 aatagagaaa tgacacgttt ggaaatggct tattatgcct gtagcactag tttaggacat
     4621 tttagactag ggattgtgga aacttattat gcaccgccgg aaatggtgtt agaacactat
     4681 ttaaatacgt ttggcgagga atatgaagga ttcgcggatg aaacgaagcg aggacgttag
     4741 aaagtccttt tgccgtgccg atggcacggc tttttgcttg gggaaaataa aaacttggaa
     4801 caaaaactgg aaagataata ctagttaaat gtagaataga agttagaaga agtacgttct
     4861 aacttttgga caaacattgg tatatcaatg tttttgatag atttcgagat tctaggtttt
     4921 aaaaattgga ggtggaaaca tggaatcaaa ccaagaagca aaagaatata aaatgaaaga
     4981 agtttccgag attacaggtc tttcgaatga cttgctccgt acgtatgaag aagaatttaa
     5041 tttgcaaatt gcgaggacat cgggagggca tcgccgctat accgaggaag atataaatac
     5101 atttgtaacg ataaagaaga agatacaaga acaaaattgg agttacaaaa aaattcgttc
     5161 ttggctctcg ggagaagaaa tgtccgtgcc gattcaagag catcaagtcg tttcaaactt
     5221 agaaaaaaag ctggataaac aggcagaaat gattcaagat ttaacggaca aactggatca
     5281 aagcttgact ttacaagcgg cgatgatgca aaaaatgatg gaattgcaaa cagaaaagaa
     5341 acaacttcaa acgattgtag agaacagaaa tcaagatttc ataaacactt tgatagaaga
     5401 aaagagaaaa gatcgagaag aacgtgagaa caaaaaacca ctatttaaac gtttgtttgg
     5461 tagataatat gtaaatttga aaaaacgaaa aaatcgatag atattttttg ttttttcaaa
     5521 ttccaaaaac ccgttcatga gaacgggttt ttctttggtc tagatttgta acgattccac
     5581 tgtattctat gttctttgaa atgaattggt gatttgtcta tatgtatctc cctaaatcag
     5641 gtttccaatc tgatttttta tagggagtgt ttaagtagtg gagtacattt tatgtattcg
     5701 ttataaaaaa cgtagattgt ctatgtaaaa agagtccttc acgatcagtt tcccatcccg
     5761 atctttcttg tttttatctt ggtgaaggac tgtctcacga agcatcaaaa aactcaaaaa
     5821 gaaacaccaa acaatattgt atattcgcta ttgcgtacat atggaatttg tctattttgt
     5881 ataacgaaaa taaattaaaa aggtataccg aatggtatac cttttgtttt atgcccttgt
     5941 tcttgggttt ccaagccaaa accaaaggac gaataaattt ggagaataca tcgtatgaaa
     6001 aaggcttctt tttcgtgaca aaaacgtttt tttcgataca aagggaaaaa gggggtggaa
     6061 acatgactat ttacacaaaa gaagcaatta aaattcttgg tttaagtggc tctacaattg
     6121 gtcactatgt tcgtgttttg gagaatcaca aatataaatt tgaaagaatg aacggaagag
     6181 taacattttc ggaaaatgat ttggaagtca tgaagataat gaagaaagtc atacgagaaa
     6241 aaacgatttc gcaagaaaaa gcggcaaaaa tcgttcttga aaagctttta gaagagaaag
     6301 aagaagaaaa agaaatgaca atgaaagaaa tggcggcaga aataagaaaa atcagtcaaa
     6361 cattagatga attgaaaaaa tcagttgtag atatggaaga gatcaaagaa atcctcaatc
     6421 aattgtatgt aaatcagcaa aaaaagggat ttatcaaaaa aatcttcgga aaatgaaagg
     6481 agaaataaaa tttattttgt atacataaaa taatggcaaa aaactttgta tacacgttgt
     6541 atacacttga tttataggtg tatacatgta ataattagag taataaaaga tgtatacaaa
     6601 aaaggatttc ggaaatactt catgtgatag atgtatacaa catgtaaaca cagagtatac
     6661 aaagcttcgt gaaaattaat tataaaagaa aaggggtata caaaaatgac aaaacaaaca
     6721 gatacacttc aaattcgcgg gggatcagaa gacgttttac aaattaaaga gatgattgaa
     6781 aagacgggga tgacggggaa agaatttctt ccggtcatgt tgcaagcgat taaaaaagaa
     6841 ttagcggtag cgggtgcaga ggaattaaat ttcacggcag atattgaaga aattaataat
     6901 catatgttac gtgtgtacaa gcttcttgga agtattacag aacgttcaaa atcggtttta
     6961 gatacgaaaa cagagcgatt aaaagaagag gttgcagaga aaaatgaaca gctgcaacaa
     7021 gaacgtgcgg catcggaaaa gcaaaaggaa gaattcgaaa aacaattgaa agaacaacaa
     7081 gaagaaattg cgaagttgaa aaaagaatta aaagaagaaa aagatttctc ttctactata
     7141 gataaagaaa atagagagca aaagaaacaa ttgaaagtgt atacagaagt gatcgaaacg
     7201 aaaaaagatg aaaatgaacg attagaaaga cgaatggaag caaacaaaaa ggcggcagag
     7261 gaaaatgaac aactgaagaa agaaaaagaa gaattacaga agaaaaccat cgaacaagaa
     7321 aaagaaatcg aaaaacttca agagaattcg aaacgagatt tggaaatgtt ggaactaaag
     7381 ttgaagaatg aacatatgca agaaattaac ggactaaatc aaaggcatat cgaacaaatt
     7441 caaagtgaac aaaaaacacc gaaaaaagcg tcaacaacgc cgaaaacaag cggtacaaaa
     7501 ccaaaacaag cggcggaaac agaaacaaaa acaaaataat tttgtataca ggaaaaaatc
     7561 atttgtaaat cttgtataca tcttgtaaat actgatatat acaggatgta tacaatgatt
     7621 attttgatgt gtaaaaagta tacaataaga ggtgttgtta tgtatgtcaa aacgtatcaa
     7681 cccgaggata tatttgcttt ttggcggaaa attcaaagtg gaacgccggt cgaatatgat
     7741 tttaaatatg tttcattaga aaaacgtgga atgctccaat tcataattta tgtacatcga
     7801 acgaagtttt ataaaacaag aggaaataat gtagaacagt tttacgaaag cttcattcaa
     7861 aaaaagaaaa acagatttga ttcgtttttt gttttactga atgaacaagg aaaaatccaa
     7921 tctaaactat atgaacatga agtgattgat gaaatctatg aaggtattga atgtatgtat
     7981 tctacacttc cagtagaaag agatccaaac gaactcatga agaagataca acatttgaac
     8041 gaacatgaag agtacgattc atggatgaag aaattaaaga tacaaagatt caaatcatta
     8101 tttaaagaaa aatgatgttt gttttcttct aaagcaaaaa agaaaaaatg ttttattttt
     8161 ttacgatgaa tgtctcaaag gtgtactttg agacagtcgc aaaacgaaaa aaattttaac
     8221 gtaggaaaaa gggtttttaa tgtcccaaag gtatatatca aataagggag ggaaaaatca
     8281 tgcaaaaaat tggatatgca cgagtttcca gtaaagatca attattagat tctcagctta
     8341 tgatgttaga ggaatatgga tgtgatgaaa tcataactga aaaagtaaca ggtgtcgcaa
     8401 aagataggaa actattcgaa ctcgttgaaa gaatggaacc tggcgataaa ttaataattg
     8461 taagtataaa tcgcttggga aggtcaacac tacaatccat tgcgctggct gaagaaatga
     8521 gaaataaagg attagaatta gttattttgg aacatcaaat tgatacaacg acaacgaacg
     8581 gaaaattgtt ttttgaaatt atgtgtagtt ttgccgactg ggaacgttcc aatttgaagg
     8641 aaaaacagga acgtggaatt caggctgctc gtttaaaggg aaaacacctc ggaaagccac
     8701 gtcaatggac aaaatcgggt atggaagaag cggtcaaaat gtacgaagaa gaacggatcg
     8761 tgagtgaaat cgtagagata acaaaagttt ctagagcggc actgtatcga gaattaaaga
     8821 agcgaggtat tacacgtaca taatgaaaaa ggaaaatgtg aaggcagcca tgcaaggcgt
     8881 aaaacattca atgcaaaccg atggttttga gataacggaa gaacaaacaa aacttgttgc
     8941 aaaacaactt cgaaacgaaa ttacagaaga agaattctta caagaggtgc gaaaacgtat
     9001 ttcaaagggg caatgatatg ggcgagttga ttcgatttcc aaaacggatc gttgagctta
     9061 cagaagaaga acataaaaga ttcattgaat acaaagaaaa catgattgct gcggagacaa
     9121 aagcggaaat gcaatactac tacaacaaag tgaaatacat catcgagcaa gccaataaac
     9181 gaaagaaata gaaaaggggt agtaagatgg gaaatgtata cacgcaaaca tgggaagaaa
     9241 ttttagagaa tcgtccaaat atgagagctg atttcgaaca aacaaaaccg cttttagaaa
     9301 ttgaagatgt agagatttgg gactatgaag aaaaagaagt agtgaaagac tggtactcgg
     9361 gtggatacgt caaaagggac gttaaagcaa tctttgtaaa gttcaaagga gaagaaagaa
     9421 gaattcgatt ccaaacatat tttcaaccaa ctgttggaga atgggtcaca tcagatatgg
     9481 caccggtaca tgtagaagga aaatgtcatg tatgcaaaca ttttaaaaca tctattgaag
     9541 atgattcgag cagttgcact tgtattaatg tattggattt aaagtattta ctagacaaaa
     9601 tcaaacaaat gaaagagcat cgattgaaat tcattttata gaaaaggggt gtagggatgg
     9661 gggaacttta tacaatttat ttcaaagatg gattgaagaa tgaattgcaa gtcaaatttt
     9721 attataagac atatgaaaga gattatggtg atagtgattt tgcttatgat tatgaccacg
     9781 atgatgacaa tttcactcat atagattgga tcgaagtgaa aaaaaatcat attttcaaaa
     9841 ttaattataa ttttacagga tttataaata gtacatatta tgcaaatgaa gaagttcaaa
     9901 aaaatgcaaa taagtataag gatgaagttc ttgatttttg taagcaaatt acaccagggc
     9961 aaaaggttga agatggtgtg atacaagatg atatggataa tccagtagta acaacagaaa
    10021 agatcgatgc catgtttcaa aagtgtaaag aaattcgctt tagcgaagga tttggtgaac
    10081 cgcattttat agtaggtgat cccgaaatgg aatacggaat ggaatatgga agttgtgttt
    10141 tgttgttgac tgttaagaat gtatggcaaa ttaaggggga aaaagagatt gaactagagc
    10201 attttgaaat tgatttccaa acagcgggta aagaaattat ggggtattct taccttccgt
    10261 ttgatagccg tacttataaa cactttgaag aaaaatttga aaaagcattg ttgcccgaaa
    10321 taaagaaaca attgaaagtt tggcagctgc aacaactatt taaatagaaa agaggtgata
    10381 gattggaagg ggttgtgagt tgttataggg gtagcgtcag ggaaatgcgg atttacaacg
    10441 gtaagcattt gatacaagtc ttatgagaca gtcagaagtc atttaaaacg ctaatttcat
    10501 tatgtctcaa taaactctac tctattggaa ttgtgaagtg atacacttaa actaacgtac
    10561 gcaggtttgt tgaataagga cttcaaaaaa tacccttaat taagctaaaa agtccttctc
    10621 tttattaaga aggacttttt tatgtgttat tccattgtct cattcattgt aatagtttcg
    10681 cttgcttcat gaccagattc tccatgtcct ggtccaaaca aattcaatat tacaactcct
    10741 acaagaatca gtgttattcc tgcaatgcta gccatgttaa gtttttcctt ccaaactaaa
    10801 actgaaatta ctgtagtcaa aacaagtcct aaacctgccc ataatgcata tgcagtgttt
    10861 aaggggattg tttttagcga caaggataaa aagaaaaaac aaccaacgaa agctattatt
    10921 acccctattg taggatagag ttttgaaaag ccttcagatg cttttaatag ggaagagcca
    10981 ataatctccc ccactattgc aagtgctaaa tatagatatg acattattcg tcctcctgaa
    11041 tcatgtttat tagttcatca aaaacttgtg tttttagttc ttcatttaat ggtgccattc
    11101 caaatatttc tgaaaaccaa agaccatcta tggataatct tgtaattgtt attttgacag
    11161 gatcaattcc atcagttatt aactttgtat gcaaaatatc gtatctctgc cggaattcat
    11221 ctaataaatc tggattaata aacatagcag ccattagtgc ggaatttaga ttattgtttt
    11281 ctaaatcgga aaatgtagat ttaagataag ccctattcca ttttcctatt gcgttatcat
    11341 cgtcgttatt aactaacgta ttaatgcatt cgaaataatt atttatccaa tcttccacca
    11401 ttcctttaat taatgcttcc ttgctaggaa aatggtataa taatccacct ttactcacgc
    11461 cagctctttg ggcgactgcc tctaatgtga gtttgacaac tccttcttct tttacaattt
    11521 cagatgcagc aatcaatata ttttttttct tattagcatg ctttgaaata gacataataa
    11581 cctccataac ttagatacat ataatatacc gtctggacgg tttttttgtc aatcgaatta
    11641 aagctcttat ttaatatcac tctttcacat aaaaataatc caataaatta atctaaagat
    11701 tgtgaagaaa tgtacttaaa gtaaagggag ctttagttca acagtcgatt tcttaaaatc
    11761 ggctttattt atgggtgaaa attacattac cgttgtaaat ccgcattttc aggactctac
    11821 cccttatagt ccaaacaaat tttttaaagt atttataaaa aaccaccaag aagaaatgga
    11881 agggtacgaa ttttcttttt cttgggcgta tacaagcgca acagaaattc gtattaaaat
    11941 ctatgcttgt ctaaaagata ttcccgaatc tgaatactgt tttaaggaat ttatcataga
    12001 cgttcctaag acaatagaag cggaaatatg gtcacgaata gaattaatag ataatcaaat
    12061 atatgaaggt tttatttatg aggaatacgg aaataaaata atgagtgcgt tgtcgaaagg
    12121 atacgatgcg gttttgataa aattggtacg gaatttcaaa gcagggattt tggatcaatc
    12181 acaatttgaa agttcggttc ggcttttgaa atcattcaga ttgcaacaat tattctagtg
    12241 aatgaacact acattaatag atttcaaaac aaaccgctaa gacaagcgag tcataaccgt
    12301 ttcacgttcc tatgcttgtc caatcggttg attttgaaac tatcaactcc gttcttcatt
    12361 gtcataaaaa gtggcatgtc gacaagaaaa aagaaaaaaa tctagtaaat tctagatttt
    12421 tgtgtaaaag tatagacatg agaaaaagaa aaaagccccc ggtgctacca acaccaaaac
    12481 ggggacttag agaaaaatat tttgagaaaa atattctttt aacgtacttt actgtctcta
    12541 tttttatata aaaagacagt aaagtcaaga aaaagaacga acggaaaaac tacatatttc
    12601 tttttctctc ttttaaaaaa gagaggtgat aacaatggac tttatttctt tcaaggaaat
    12661 agaaagtcag gttgatagtg atgtaataga aaacatcaaa agtcagtgtt atgaacgtgt
    12721 tttttcccga agtgaactta caatagtagg gaaaacaaaa actggtacac gttatcgttc
    12781 gagaaacatt tatgaaacgg tattcaatga agataagtac aaacggatcg tgagacaata
    12841 cttcattgat catattcatc aaaaagagga aaaaccttcg aaaaaagaac aaaataaaac
    12901 aacgaaaaag actccgacga aacgaaagaa aaaagagatc ggaaaaaaca ttacgcaacg
    12961 agaatttttc gaacatattt ttcgagattt gtttgacaac aagcgtggag aagaagataa
    13021 aaagggacgt agaatctatt taaagtcatt tctttttgat attgataaag aatcaaagct
    13081 gattaatgaa aaaaaagtag taagtaatgt agagaaagcc atcaattatg ggaaaaaatg
    13141 cggttatttt acaccggcac tattcatttc tcataagttt tttacaaaag agatgctgtc
    13201 attgatgggt gtaattctct tggactttga tttggataaa gcgaacgttg ttatgacgaa
    13261 agaacaagta tataagtaca ttaaaaagaa gttaaaagta gaaccatcaa
//
