LOCUS       NZ_AP022936             3869 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain J7 plasmid pJ7_11, complete sequence.
ACCESSION   NZ_AP022936
VERSION     NZ_AP022936.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203332
            Sequence Read Archive: DRR206372, DRR206396
            Assembly: GCF_021655135.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 344x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655135.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 07:11:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,087
            CDSs (total)                      :: 5,938
            Genes (coding)                    :: 5,644
            CDSs (with protein)               :: 5,644
            Genes (RNA)                       :: 149
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 294
            CDSs (without protein)            :: 294
            Pseudo Genes (ambiguous residues) :: 0 of 294
            Pseudo Genes (frameshifted)       :: 177 of 294
            Pseudo Genes (incomplete)         :: 134 of 294
            Pseudo Genes (internal stop)      :: 43 of 294
            Pseudo Genes (multiple problems)  :: 50 of 294
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022936.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="J7"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pJ7_11"
                     /geo_loc_name="Japan:Tochigi"
                     /collection_date="2006"
     Typing          complement(1995..2536)
                     /name="rep_cluster_1931"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          744..1954
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agacaaaggc ggatccctac tctcacaagg aatccgcctt tgtctttttc tatgattcta
       61 tgcatcttct tatacattcc tattatggcg ggcttttcgg cctccccata gttccctatg
      121 tcttccgatt ttttgcataa aatcttgcat actcttagcg tggttttttt caaaatgctg
      181 gtggtactcc ttaataacaa gttcatagaa tgttgatttt ataaatcaaa agagagggga
      241 acctatgtag tgctacttga gtagaaaatt aggtttttaa ttaggtagag gtagctgcat
      301 agcgtgtgtc aacggtaccg gaccgaaccc gtaagggggc gggaggataa ggagttgaca
      361 cactcactat gcaaccctct tgtggttgtc aaaaggtacc cttttgactc tgccaaccgg
      421 cgagggagcc cctcaaggat tgaggggttg gggagttgga ttaagggggt ctggggaaga
      481 gttccccagc aggtttggac agagtccaag gttttgtttt ttgtcatgca aagcatggct
      541 tggcgatagc caaaacagcc tcgcagagcc ttttcattgt gtacatggag cgtagcggaa
      601 tgtcacaata cagaaaaggt tactttgcca aggtaacaaa gcttttggta tagtggaacc
      661 aaggagttga ttcgttatga gtatgtcagt ttctctcaag aaggcaacga acaaaaccaa
      721 cattagacac aataacagga caatgagcga gaaggaaaaa gagcaaaatt cgcacataga
      781 ttattcaagg tctgacgaaa ataaatatct tgttcagaaa gatttaaaga aattatatcg
      841 agaagaattt ggagaagtgt tggagaacta taacgccaaa caaaaacgta acgaccgaaa
      901 aattgatgac tattataaac acattcagtc cagtaaaaaa acatcacttc agcaagaaat
      961 gattattcaa gttggcgata aagatgattt ttctagtaaa caagattttg aaaaggctaa
     1021 cgaaatttta gaagaatggt tattgggatt tgaaaaacga aatccaaatt taaaaattta
     1081 taacgcagtc attcataatg atgaagcgag cccacacatg catttgaatt ttgttccagt
     1141 agcaagcggg tataaacgtg gacttgaaaa gcaagtatca tttgaccgag caattacgca
     1201 acaagatcca aaattagata aaactcgtcc ttttgatgat tggcgagaaa aagaagtgaa
     1261 gatattagaa gaaatgttaa aagagcgtgg tatcgaacgg aaattagttg gttcaaataa
     1321 ttataaagat gtgaatgaat acaaagaaaa aaaggattta gaaaaggaaa ttctttcttt
     1381 ggaaaaccag ctttttgaaa agaaaaatga attactggaa atgaacgaac gattgccagg
     1441 agaaattaag attaaagctg agagagaaaa gaaaacagag gttgttaata aaggtttttt
     1501 aaaaaaagaa aaagtagaaa aaccaacagg gaattggatt gttgaaacta aagaattaaa
     1561 aagagtgcaa ggaataatta acgctggata ttcagtgaaa aaagattatg atcgtttgca
     1621 gcgtatggat ttagttaaag aaaacaaaga attatgtgac aaagttgata gtttagctga
     1681 aggttatgtt aaagctatta acgaaaatac agatttggaa aatgagaata gagaattgcg
     1741 taaggaaata agctcgttaa aagcccatat aagagattta aaggaaaatg taaaggtttt
     1801 ataccataac acaaaaaaac tcttaggaag gcaatttgag ggctttagag gtcttattaa
     1861 gaacgagttg gatatgaaag ggatagataa tcaatttgag cgtgaacata aaaaagaaat
     1921 gaagactaaa caacgagggt atgacatgga gcgatgaaaa cataaataaa gcgagtagac
     1981 tttactaccc gctttatttt aggttttccc gttttcgcaa agctttaaat ctatcaaatt
     2041 gtttacgata tatatagtct ttattttctt ttttgctgac taagattttc ccgtcgtatt
     2101 ttgtaaattt tttcttgtcg tttgaatcag tccacgctaa atcttcattt actaaataaa
     2161 catttgatgt acccattttt agtacactca aaaatccgtt ctcttcaagt actttgattc
     2221 ctctataaat agtcattttt gattttccaa agtaatcttc aaaagttgaa tatgagcatg
     2281 ctaatgcatt cttgttatcc atgtgttcga ttatgaaaaa taaaagagaa ctagcgaaat
     2341 tgtggtttgc cattaaccat cttagttctg gcatgttatc tcgataaagt tgaatgaaat
     2401 taagattctt tttttcactt tcttttttct gttgatcttg ttcatttttt tcatctagtt
     2461 ctaataattt ttgaagtttt ttaaattcat ccgcgtttaa ttctatagtg attttttctt
     2521 gggagttctt gtccattttt ccaccccttt tttgcaagaa ggtatcacac tatgatacct
     2581 atatgtataa tctttgttat atcctataac atgacctgat atatgtaaag acaaatttgt
     2641 tatattcttc tacaaatagg tatcacaagc tatgttacct ataggtatca cactatgata
     2701 cctattaaac tctgaaaccc ttatatatca aggcttctct tacgttccct ttctatatct
     2761 cctaataggg tttttctcca cgcaatgggc aggtagtatt ttttgagaaa cccactcaaa
     2821 aaatctccac cttttttcca ttgcattccg aaattcggct ggtttttagc tttcgccaag
     2881 ccgaacagac caaaaacgag gttaagttgc ccgacttgga ccagcaattg taccagccga
     2941 accacccagt tttatcgatc taagcaataa gcttactcta tattcagtta cgttaagttc
     3001 ttaggggcta gcccctcgct tcgcttcacc ccagccttca ccactccacc caaaaacaag
     3061 acgatcgttt aaaataaaaa ataaaataag ctgtccatat ggacagctta tttacataaa
     3121 attcgaaagc ggaagtcaat aaatctatca tatatcttca gcgatttatt gctgaagata
     3181 caactgtttt gataatggaa tacccttatt gaaaaattct gagtaatcgt ttaaataata
     3241 atctgctaca tcactttgac cttgaaacat gcatgttatg attcctaatt cttttgcagg
     3301 tagcaaatct aattctctat ctccaatagc tagatcaatt ttgtgttttt gatgcaagta
     3361 agtatatgct gatgggttgg gtttgcgagg aaaaccatta tcaatcgtaa ccatatctac
     3421 aaagtatttg tcccatccat aatattttaa aattgctaaa actccatctc tgtgtttatg
     3481 tgtcatgatt acattcttat ctgcaaattt caaaatttct tccacgccat caaatggttt
     3541 catgtctttt ggagacaatt ccttttttaa gcttttaatt ttctctttct ggtcactaga
     3601 aacattatag tagtctagtg catgtgagta tgaaatcttt aattttttat aaatctcctg
     3661 tttatctatc tcttctccaa gtactagaga gagtattttg gtataggcag gatatgtatt
     3721 aaaaagtgtt ccgtcaaagt cccataaaat attcaattaa atcacccttt ctattccatt
     3781 ttatcacaag aatgtggtag ccatactctt gttttctttg tatactccat acactatcag
     3841 ttaacataac gtctcattat cggtagtga
//
